CN107467413A - The preparation method of loach scrod feed - Google Patents
The preparation method of loach scrod feed Download PDFInfo
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- CN107467413A CN107467413A CN201710639445.8A CN201710639445A CN107467413A CN 107467413 A CN107467413 A CN 107467413A CN 201710639445 A CN201710639445 A CN 201710639445A CN 107467413 A CN107467413 A CN 107467413A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses the preparation method of loach scrod feed, including activation culture, bean pulp solid-state fermentation, the preparation of feed.Have the beneficial effect that：Bosom ovum phase loach feed prepared by the present invention is using fermented bean dregs as protein sources, with bafillus natto to bean pulp fermentation processing, various antigenic components, ANFs in dregs of beans is set to be can be effectively reduced removal, protein in dregs of beans is also broken down into substantial amounts of small peptide, this nonantigenic small peptide is more easy to be absorbed by loach, can significantly improve the survival rate of loach, while reduces residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Description
Technical field
The present invention relates to aquatic feeds field, more particularly, to the preparation method of loach scrod feed.
Background technology
Loach is subordinate to Cypriniformes Cobitidae loach genus, is that one kind blazons small-sized bottom and dwelt ominivore-fish.Due to special
Intestinal respiration function, loach lower oxygen concentration resistance easily transport, strong environmental adaptability, can almost be cultivated in various waters.Loach
Market demand is more stable, and demand for exports amount is larger, and the market price is high, high financial profit.But recently as chemical fertilizer, agriculture
The pollutions such as medicine, industrial wastewater aggravate, and the field ecological environment of loach further deteriorates, and plus the overfishing of the mankind, causes open country
Raw loach resource falls sharply.The yield of wild loach far can not meet the market demand, and development intensive culture is to meet people
The effective way of class demand, and feed is the basis for developing intensive culture.
Prior art such as Authorization Notice No. is the B of CN 104171570 Chinese invention patent, discloses a kind of loach and coordinates
Feed, nutrition is balanced can to make loach be readily adapted to accommodate breeding environment comprehensively, but the feed is non-digestible, and easily in water body
Middle residual, in turn result in the pollution-free of environment.
The content of the invention
It is an object of the invention to provide the preparation method of loach scrod feed, feed absorption easy to digest prepared by the method,
The survival rate of loach can be significantly improved, and it is small to water pollution.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：The preparation side of loach scrod feed
Method, including activation culture, bean pulp solid-state fermentation, the preparation of feed.The cost of raw material is cheap used in preparation process, is easy to store up
To deposit, the ratio between composition is moderate, and prepared feed palatability is good, is easily digested absorption, is capable of the survival rate of notable loach,
And remained in water body less, it is small to water pollution.
Preferably, activation culture process is：Bafillus natto is inoculated in inclined-plane in a manner of sterile working first
Culture medium, 30-33 DEG C of culture 2-3d, the composition and its parts by weight of slant medium are：Beef extract 0.3-0.5 parts, peptone 1-
1.3 parts, sodium chloride 0.5-0.7 parts, agar 2-3 parts, water 90-95 parts；Then liquid is accessed from slant medium picking 1-2 rings bacterium
In activation medium, 34-37 DEG C, 170-180r/min shaken cultivation 6-7h, the composition and its parts by weight of liquid activation medium
For：Beef extract 1.5-2.5 parts, peptone 5-7 parts, sodium chloride 2.5-3.5 parts, water 400-450 parts.Bafillus natto used
Buy in Chinese agriculture Culture Collection.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more steady
It is fixed, purebred, the shortening process cycle of certain amount and quality can be quickly obtained, and be follow-up bean pulp solid-state fermentation process
Sufficient amount of seed is provided.
Preferably, bean pulp solid-state fermentation process is：By the bafillus natto bacterium solution obtained by activation culture process with
10% inoculum concentration accesses solid medium, adds 0.2-0.4 part active peptides, 35-37 DEG C of culture 60-70h, obtains fermentation beans
The dregs of rice.The preparation method of solid medium is：With plant pulverizer grind dregs of beans, take 7-9 parts dregs of beans, 0.5-1.3 parts wheat bran and
2-3 parts water mixes, and stands 2h, gained mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains solid medium.This
Under part, solid medium utilization rate is high, and probiotics in tunning, oligopeptides, glutamic acid, the content of lactic acid and vitamin are big, can
To improve animal body immunity, suppress the generation of disease of digestive tract, improve the survival rate of loach.
Preferably, the amino acid sequence of the active peptides added during bean pulp solid-state fermentation is：
HSHACASYSCRARRKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Bafillus natto is in solid-state
Exotoxin can be synthesized in fermentation process, influences its metabolism, the bioactive peptide of addition is combined with ectotoxic active site, had
Ectotoxic poisonous effect is reduced to effect, favorable environment is provided for solid state fermentation culture.
Preferably, the preparation process of feed is：Take white fish meal 12-16 parts, fermented bean dregs 20-24 parts, fish oil 3-5 parts,
Soybean oil 3-5 parts, Strong flour 40-45 parts, Choline Chloride 0.1-0.3 parts, calcium dihydrogen phosphate 0.8-1.2 parts, bile salt
0.1-0.2 parts are well mixed, by coarse-cracking machine and micronizer, sorting, granulation, curing, drying feed.Under the conditions of being somebody's turn to do
Prepared feed nutrition is comprehensive, and protein sources are mainly nonantigenic small peptide, are easily digested and assimilated by animal, reduces feed in water
Residual in body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Compared with prior art, the advantage of the invention is that：The process that the present invention prepares feed includes activation culture, dregs of beans
The preparation of solid state fermentation and feed, the feed, with bafillus natto to bean pulp fermentation processing, make using fermented bean dregs as protein sources
Various antigenic components, ANFs in dregs of beans are can be effectively reduced removal, and the protein in dregs of beans is also broken down into largely
Small peptide, this nonantigenic small peptide are more easy to be absorbed by loach, can significantly improve the survival rate of loach, while reduce feed and exist
Residual in water body, reduce the ammonia-nitrogen content in water body, meet green requirement.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
The preparation method of loach scrod feed, comprises the following steps：
1) activation culture：Bafillus natto is inoculated in slant medium first in a manner of sterile working, 30 DEG C are cultivated 2d,
The composition and its parts by weight of slant medium be：0.3 part of beef extract, 1 part of peptone, 0.5 part of sodium chloride, 2 parts of agar, water 90
Part；Then accessed from the ring bacterium of slant medium picking 1 in liquid activation medium, 34 DEG C, 170r/min shaken cultivation 6h, liquid
The composition and its parts by weight of activation medium be：1.5 parts of beef extract, 5 parts of peptone, 2.5 parts of sodium chloride, 400 parts of water.Used
Bafillus natto is bought in Chinese agriculture Culture Collection；
2) bean pulp solid-state fermentation：By the bafillus natto bacterium solution obtained by activation culture process with the access solid training of 10% inoculum concentration
Base is supported, 0.2 part of active peptides is added, 35 DEG C of culture 60h, obtains fermented bean dregs.The preparation method of solid medium is：Use plant
Pulverizer grinds dregs of beans, takes 7 parts of dregs of beans, 0.5 part of wheat bran and 2 parts of water mixing, stands 2h, gained mixture is placed in into blake bottle
In, 121 DEG C of sterilizing 25min, obtain solid medium.The amino acid sequence of active peptides is：
HSHACASYSCRARRKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR；
3) preparation of feed：Take 12 parts of white fish meal, 20 parts of fermented bean dregs, 3 parts of fish oil, 3 parts of soybean oil, 40 parts of Strong flour, chlorine
Change 0.1 part of choline, 0.8 part of calcium dihydrogen phosphate, 0.1 part of bile salt is well mixed, by coarse-cracking machine and micronizer, divides
Choosing, granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, and protein sources are mainly nonantigenic small
Peptide, easily digested and assimilated by animal, reduce residual of the feed in water body, reduce the ammonia-nitrogen content in water body, meet green
The requirement of environmental protection.
Embodiment 2：
The preparation method of loach scrod feed, comprises the following steps：It is activation culture first：Will first in a manner of sterile working
Bafillus natto is inoculated in slant medium, and 33 DEG C of culture 3d, the composition and its parts by weight of slant medium are：Beef extract
0.5 part, 1.3 parts of peptone, 0.7 part of sodium chloride, 3 parts of agar, 95 parts of water；Then liquid is accessed from the ring bacterium of slant medium picking 2
In body activation medium, 37 DEG C, 180r/min shaken cultivation 7h, the composition and its parts by weight of liquid activation medium are：Beef
2.5 parts of cream, 7 parts of peptone, 3.5 parts of sodium chloride, 450 parts of water.Bafillus natto used is bought in Chinese agriculture microorganism
DSMZ.Under the conditions of being somebody's turn to do, the growth vigor of strain becomes strong, and physiological character is more stable, can quickly obtain certain
Purebred, the shortening process cycle of quality and quantity, and provide sufficient amount of seed for follow-up bean pulp solid-state fermentation process；Connect
It is bean pulp solid-state fermentation：Bafillus natto bacterium solution obtained by activation culture process is accessed into solid culture with 10% inoculum concentration
Base, 0.4 part of active peptides is added, 37 DEG C of culture 70h, obtains fermented bean dregs.The preparation method of solid medium is：Use Plant Powder
Broken machine grinds dregs of beans, takes 9 parts of dregs of beans, 1.3 parts of wheat brans and 3 parts of water mixing, stands 2h, gained mixture is placed in into blake bottle
In, 121 DEG C of sterilizing 25min, obtain solid medium.Should under the conditions of, solid medium utilization rate is high, probiotics in tunning,
Oligopeptides, glutamic acid, the content of lactic acid and vitamin are big, can improve animal body immunity, suppress the generation of disease of digestive tract,
Improve the survival rate of loach；It is finally the preparation of feed：Take 16 parts of white fish meal, 24 parts of fermented bean dregs, 5 parts of fish oil, soybean oil 5
Part, 45 parts of Strong flour, 0.3 part of Choline Chloride, 1.2 parts of calcium dihydrogen phosphate, 0.2 part of bile salt is well mixed, by coarse powder
Machine and micronizer, sorting, granulation, curing, drying feed.Prepared feed nutrition is comprehensive under the conditions of this, albumen
Source is mainly nonantigenic small peptide, is easily digested and assimilated by animal, reduces residual of the feed in water body, reduces in water body
Ammonia-nitrogen content, meet green requirement.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City You Ze feed technologies Services Co., Ltd
<120>The preparation method of loach scrod feed
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 57
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Ser Cys Arg Ala Arg Arg Lys Ala
1 5 10 15
His Cys Arg Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser His Arg Gly
20 25 30
Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys
35 40 45
Leu Cys Val Cys Val Asn Cys Ser Arg
50 55
Claims (7)
1. the preparation method of loach scrod feed, including activation culture, bean pulp solid-state fermentation, the preparation of feed, it is characterised in that：
The bean pulp solid-state fermentation process is：Bafillus natto bacterium solution obtained by activation culture process is solid with the access of 10% inoculum concentration
Body culture medium, 0.2-0.4 part active peptides are added, 35-37 DEG C of culture 60-70h, obtain fermented bean dregs.
2. the preparation method of loach scrod feed according to claim 1, it is characterised in that：The system of the solid medium
Preparation Method is：Dregs of beans is ground with plant pulverizer, 7-9 parts dregs of beans, 0.5-1.3 parts wheat bran and the mixing of 2-3 parts water is taken, stands
2h, gained mixture is placed in blake bottle, 121 DEG C of sterilizing 25min, obtains solid medium.
3. the preparation method of loach scrod feed according to claim 1, it is characterised in that：The ammonia of described active peptides
Base acid sequence is：HSHACASYSCRARRKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
4. the preparation method of loach scrod feed according to claim 1, it is characterised in that：The process of the activation culture
For：Bafillus natto is inoculated in slant medium, 30-33 DEG C of culture 2-3d in a manner of sterile working；Then from inclined-plane
In culture medium picking 1-2 rings bacterium access liquid activation medium, 34-37 DEG C, 170-180r/min shaken cultivations 6-7h.
5. the preparation method of loach scrod feed according to claim 3, it is characterised in that：The slant medium into
Divide and its parts by weight are：Beef extract 0.3-0.5 parts, peptone 1-1.3 parts, sodium chloride 0.5-0.7 parts, agar 2-3 parts, water 90-
95 parts.
6. the preparation method of loach scrod feed according to claim 3, it is characterised in that：The liquid activation medium
Composition and its parts by weight be：Beef extract 1.5-2.5 parts, peptone 5-7 parts, sodium chloride 2.5-3.5 parts, water 400-450 parts.
7. the preparation method of loach scrod feed according to claim 1, it is characterised in that：The preparation method of the feed
For：Take white fish meal 12-16 parts, fermented bean dregs 20-24 parts, fish oil 3-5 parts, soybean oil 3-5 parts, Strong flour 40-45 parts, chlorination
Choline 0.1-0.3 parts, calcium dihydrogen phosphate 0.8-1.2 parts, bile salt 0.1-0.2 parts are well mixed, by coarse-cracking machine and ultra micro
Pulverizer, sorting, granulation, curing, drying feed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710639445.8A CN107467413A (en) | 2017-07-31 | 2017-07-31 | The preparation method of loach scrod feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710639445.8A CN107467413A (en) | 2017-07-31 | 2017-07-31 | The preparation method of loach scrod feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108244405A (en) * | 2018-01-10 | 2018-07-06 | 广西平南县平原农牧有限公司 | A kind of Chinese snake head special feed |
| CN110178974A (en) * | 2019-04-09 | 2019-08-30 | 晟丰(烟台)农业科技有限公司 | Feed addictive, feed and preparation method thereof |
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|---|---|---|---|---|
| CN102008006A (en) * | 2009-09-08 | 2011-04-13 | 中国农业科学院饲料研究所 | Preparation method of polypeptide for fermented feed with molecular weight of less than 15KDa |
| CN104000050A (en) * | 2014-06-18 | 2014-08-27 | 通威股份有限公司 | Loach settling-type puffing mixed feed and preparing method thereof |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104664130A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Polypeptide-containing aquatic microbial feed |
| CN105851704A (en) * | 2016-04-29 | 2016-08-17 | 宿松富民水产养殖有限公司 | High-survival-rate feed for 15-45-day-old loach |
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2017
- 2017-07-31 CN CN201710639445.8A patent/CN107467413A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008006A (en) * | 2009-09-08 | 2011-04-13 | 中国农业科学院饲料研究所 | Preparation method of polypeptide for fermented feed with molecular weight of less than 15KDa |
| CN104093734A (en) * | 2011-12-21 | 2014-10-08 | 明尼苏达大学董事会 | Cationic peptides and use of such peptides for inhibiting exotoxin production |
| CN104664130A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Polypeptide-containing aquatic microbial feed |
| CN104000050A (en) * | 2014-06-18 | 2014-08-27 | 通威股份有限公司 | Loach settling-type puffing mixed feed and preparing method thereof |
| CN105851704A (en) * | 2016-04-29 | 2016-08-17 | 宿松富民水产养殖有限公司 | High-survival-rate feed for 15-45-day-old loach |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108244405A (en) * | 2018-01-10 | 2018-07-06 | 广西平南县平原农牧有限公司 | A kind of Chinese snake head special feed |
| CN110178974A (en) * | 2019-04-09 | 2019-08-30 | 晟丰(烟台)农业科技有限公司 | Feed addictive, feed and preparation method thereof |
| CN110178974B (en) * | 2019-04-09 | 2021-09-07 | 晟丰(烟台)农业科技有限公司 | Feed additive, feed and preparation method thereof |
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Application publication date: 20171215 |