CN103705773A - Traditional Chinese medicine composition for achieving antibacterial and anti-inflammation effects and preparation method thereof - Google Patents
Traditional Chinese medicine composition for achieving antibacterial and anti-inflammation effects and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for achieving antibacterial and anti-inflammation effects and a preparation method of the traditional Chinese medicine composition. The preparation method comprises the steps of taking eight medicinal materials including Desmodium styracifolium, corn stigma, pyrrosia lingua, Abrus cantoniensis Hance, Poria cocos, plantain herb, Lygodium japonicum Sw and lalang grass rhizome, wherein the Desmodium styracifolium, the pyrrosia lingua, the Lygodium japonicum Sw, the Abrus cantoniensis Hance and the plantain herb are extracted by an alkaline aqueous solution, and the lalang grass rhizome, the Poria cocos and the corn stigma are extracted by water; and finally combining fluid extracts obtained by the two extraction methods. The through animal pharmacological experiments and clinical test results prove that the traditional Chinese medicine composition provided by the invention has excellent antibacterial and anti-inflammation effects superior to the prior art when being used for treating lithiasis in urinary system.
Description
Technical field
The present invention relates to treat the Chinese medicine composition of urinary system calculus, be specifically related to a kind of anti-inflammation Chinese medicine composition and preparation method thereof.
Background technology
Urinary system calculus belongs to " sand Stranguria ", " stranguria caused by urinary stone " and " stranguria with blood " scope in traditional Chinese medical science gonorrhea, mainly comprises 4 kinds of pattern of syndrome:
(1) qi stagnation and blood stasis type: disease is seen waist dull pain, dull pain, the normal or stringy and tense pulse of arteries and veins, tongue is normal; Or urine is interrupted suddenly when excessive, sharp ache, the upper waist abdomen that connects, sandstone discharge after pain slow; Or waist, flank portion tugging pain be as strand, pain extending to the lower abdomen, outbreak again and again, pale complexion, cold sweat, vomiting and nausea during pain, companion's hematuria or urine yellow skin are red, dimly red tongue or have petechia, ecchymosis, stringy and tense pulse or slow puckery.
(2) damp heat downward flowing type: card is shown in fever with aversion to cold, lumbago, few abdomen is anxious full, and frequent micturition is short red, and while drowning, puckery pain unbearably, is drenched drop not well.Yellow and greasy fur, stringy and rolling pulse or sliding number.
(3) deficiency of the kidney yin type: calculus is stopped for a long time, pathogenic heat are strongly fragrant for a long time.Kidney-Yin loss, dizzy tinnitus, pain in the low back and legs, dribbling urination or not well, insomnia and dreamful sleep, time have low grade fever, shortness of breath and palpitation, dysphoria with feverish sensation in the chest palms and soles, night sweat, eye is dry or puckery, abdominal distention constipation, poor appetite, thready and rapid pulse, red tongue or few tongue.
(4) kidney yang deficiency syndrome: calculus is stayed for a long time, kidney yang deficiency, the acid of waist lower limb is heavy, lassitude, whole body is timid cold, and extremities being not warm or the lower part of the body often have creeping chill, frequent micturition or dysuria, frequent urination at night, complexion is white, a little less than deep-thready pulse, the white pale tongue of tongue.
The 13rd disclosed JIESHITONG PIAN of < < the Sanitation Ministry medicine standard > > discloses following information:
1) form: Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperatae.
2) method for making: above eight tastes, to get Poria 96g and be ground into fine powder, seven tastes such as residue Poria and all the other Stigma Maydis decoct with water secondary, each 3 hours, collecting decoction, filtered, filtrate is condensed into the clear paste that relative density is 1.19-1.20 (90 ℃), adds Poria fine powder, mixes, dry, pulverize into fine powder, sieve, add appropriate amount of auxiliary materials, granule processed, mixes, be pressed into 1000, sugar coating, obtains.
3) function with cure mainly: clearing away heat-damp and promoting diuresis, Tonglin Paishi, pain easing and hemostasis.For urinary system infection, cystitis, oedema due to nephritis, lithangiuria, hematuria, drenches drop muddy, urethra causalgia etc.
But the proportional quantity that there is no clear and definite each composition in described ministry standard.
About the logical extracting method of calculus, there is following report:
Chinese patent application 01100318.9 is clear and definite, and the logical preparation method of calculus is: all pulverizing medicinal materials are nanoscale, microwave extracting, and concentrating under reduced pressure, dry.
Chinese patent application 200510032580.3 discloses the preparations such as the logical ball of calculus, drop pill, micropill, dispersible tablet, pellet capsule.The disclosed preparation method of this patent adopts part Poria to pulverize, residue Poria and all the other 7 taste medical material water extractions, or whole medical material water extraction.
Chinese patent application 200510047400.9 discloses a kind of Chinese medicine sugar free granule that is used for the treatment of lithangiuria and preparation method thereof.This patent raw material is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, and residue Poria and all the other 7 taste medical material water extractions, adopt low temperature drying method.
Chinese patent application 200910224023.x discloses the treatment pharmaceutical composition of diseases of urinary system and the detection method of component thereof.The raw material that this patent is used is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.The detection method of Herba Abri, chlorogenic acid in preparation is disclosed.
Chinese patent application 200910249899.x discloses the preparation method of pharmaceutical composition and the detection method of component thereof for the treatment of diseases of urinary system.The raw material that this patent is used is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.The detection method of Folium Pyrrosiae in preparation is disclosed.
Because the character of active component in eight taste medical materials is different, as preparation method in above-mentioned patent all adopts water extraction, can cause some active component to propose, or extraction ratio is low, affect the performance of drug effect.
Therefore, need searching that a kind of preparation method that can improve medicine antibacterial and anti-inflammation functions is provided.
Summary of the invention
The anti-inflammation Chinese medicine composition that the object of this invention is to provide a kind of determined curative effect.
A kind of anti-inflammation Chinese medicine composition provided by the invention, the preparation method of its active component comprises the following steps:
1) get the medical material of following weight portion: 456 parts of Herba Desmodii Styracifolii, 302 parts of Stigma Maydis, 228 parts of Folium Pyrrosiaes, 228 parts of Herba Abris, 228 parts, Poria, 165 parts of Herba Plantaginiss, 165 parts of Herba lygodiis, 228 parts of Rhizoma Imperataes;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis are used alkaline aqueous solution to extract for extracting solvent, and extract is made fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water, extract is made fluid extract;
4) by step 2), 3) all fluid extracts of gained two mix, obtain.
Above described preparation in anti-inflammation Chinese medicine composition step:
Described step 2) neutral and alkali aqueous solution is preferably: the sodium bicarbonate solution that quality-percent by volume is 0.1-5%.
Described step 2) alkaline aqueous solution more preferably: the sodium bicarbonate solution that quality-percent by volume is 0.5-1%.
Described step 2) extracting method is more preferably:
Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis add alkaline aqueous solution extracts 2-3 time, adds the alkaline aqueous solution that medical material gross weight 10-20 doubly measures at every turn, and each extraction time is 1-4 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.
Described step 3) is preferably:
Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2-3 time add the water that three taste Chinese medicine gross weight 6-12 doubly measure at every turn, decoct 1-3 hour at every turn, and collecting decoction filters, and filtrate is condensed into fluid extract.
Described step 3) is more preferably:
Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2-3 time add the water that three taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct 2-3 hour at every turn, and collecting decoction filters, and filtrate is condensed into fluid extract.
Described step 3) is further preferably:
Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2 times add the water that three taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct 2-3 hour at every turn, and collecting decoction filters, and filtrate is condensed into fluid extract.
The present invention also provides the preparation containing above-mentioned Chinese medicine composition, and said preparation is made separately by Chinese medicine composition or is comprised of Chinese medicine composition and pharmaceutically acceptable carrier.
Described preparation is solid preparation or liquid preparation, and solid preparation is tablet, capsule or granule; Liquid preparation is mixture or oral liquid.
Described pharmaceutically acceptable carrier is one or more in lactose, starch, dextrin, mannitol, sucrose, hydroxypropyl methylcellulose, cross-linking sodium carboxymethyl cellulose, low-substituted hydroxypropyl cellulose, microcrystalline Cellulose, micropowder silica gel, pregelatinized Starch, sorbitol, magnesium stearate, Pulvis Talci, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sodium benzoate, potassium sorbate, sucrose or aspartame, monosodium glutamate, Mel, orange flavor.
The preparation method of Chinese medicinal composition preparation of the present invention, the method can adopt conventional formulation technique to be prepared as various common dosage forms.
For example:
Method 1: Chinese medicinal composition capsules agent of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, be dried to dried cream powder, add capsule to commonly use adjuvant, production method is prepared into the capsule of Chinese medicine composition of the present invention routinely.
Method 2: Chinese medicine composition tablet of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, be dried to dried cream powder, add tablet to commonly use adjuvant, production method is prepared into the tablet of Chinese medicine composition of the present invention routinely.
Method 3: Chinese medicinal composition granules of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, be dried to dried cream powder, add granule to commonly use adjuvant, production method is prepared into the granule of Chinese medicine composition of the present invention routinely.
Method 4: Chinese medicine composition mixture of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, add mixture to commonly use adjuvant, production method is prepared into the mixture of Chinese medicine composition of the present invention routinely.
Method 5: Chinese medicine composition oral liquid of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, add oral liquid to commonly use adjuvant, production method is prepared into the oral liquid of Chinese medicine composition of the present invention routinely.
Method 6: Chinese medicine composition syrup of the present invention
Get Chinese medicine composition preparation process 4) middle gained Chinese medicine composition, add syrup to commonly use adjuvant, production method is prepared into the syrup of Chinese medicine composition of the present invention routinely.
The application that the present invention also provides above-mentioned Chinese medicine composition or preparation to improve anti-inflammation effect when preparation treatment urinary system calculus disease.
Urinary system calculus belongs to stranguria caused by urinary stone, sand Stranguria in traditional Chinese medical science gonorrhea, due to damp invasion of lower energizer, changes the scorching the moon that burns, and decocts urine, becomes sandstone, alluvial water channel, and be stranguria caused by urinary stone.Amass in next bladder qi dysfunction, it is unfavorable to urinate, very want can not, poverty-strickenly feel bad, pain extending to the lower abdomen; Be stranded in, affect the duty of kidney department urine, pent-up must not be let out down, and QI and blood is unsmooth, and stagnation of QI and blood may bring about pain is involved bladder, pudendum by kidney; It is hematuria that sandstone is hindered network.Therefore, treatment stranguria caused by urinary stone should be when washing away sandstone, diuresis promoting, and the infection symptoms that urinary system is occurred is treated in time, prevents that inflammation from further expanding, and has influence on other normal organs.
Chinese medicine composition effective ingredient disclosed in this invention is to be prepared from by raw materials such as Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperataes.Inventor finds in preparation research process for many years, identical drug dose, different extraction processes are prepared the active component of gained, also can there is larger difference and emphasis in its pharmacological action, for this reason, applicant has carried out a series of technical study to Chinese medicine composition of the present invention in conjunction with pharmacological evaluation, sum up process for extracting of the present invention, and through the such improvement of experimental verification, brought drug effect potentiation: work as Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis is used alkaline aqueous solution to extract for extracting solvent, Rhizoma Imperatae, Poria, Stigma Maydis medical material water extraction, above two kinds of extracts merge gained Chinese medicine composition, while being used for the treatment of urinary system calculus, its anti-inflammation effect is far superior to the existing extractive technique of identical prescription, clinical efficacy shows, extract of the present invention compared with prior art, there were significant differences for antiphlogistic antibacterial curative effect, there is significance progress.
Inventor detects Chinese medicine composition of the present invention, find: Chinese medicine composition general flavone content of the present invention is very high, compare with existing extraction process products obtained therefrom, both have notable difference, may be because flavone component in Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis is soluble in alkaline aqueous solution, so extract more complete in alkaline aqueous solution.
Above-mentioned testing result has shown that in each component, the extraction of some effective ingredient is better than prior art from the side.Because every taste Chinese crude drug all contains many active component, at least not yet there is any a herb purely and simply to be analyzed and know all the components, different Chinese medicine combines while extracting with different extracting modes, due to influencing each other and acting between solvent, temperature and heterogeneity, can produce very complicated chemical reaction.Therefore, above-mentioned effective ingredient is a part for Chinese medicine extract active component, for the drug action impact of whole medicine, is still not clear.We can only contrast different extracting modes in the quality aspect certain therapeutic use by effect experiment at present, and the method that there is no judges that from the change indicator of part effective ingredient the curative effect of compound Chinese patent medicine changes.
We contrast different extracting modes in the curative effect aspect antiinflammatory by " Ovum Gallus domesticus album being caused to the impact of rat toes swelling " zoopery; By " paper disk method extracorporeal bacteria inhibitor test ", contrast different extracting modes in the curative effect of antibiosis.
Inventor has carried out a large amount of systematic research experiments to extracting method and the excellent effect of medicine of each logical medical material of calculus, by medical material is split as to many groups, with diverse ways, extract, finally obtained extractive technique scheme of the present invention, make the medical material of same recipe, given play to that therapeutic purposes are clearer and more definite, the effect of more remarkable treatment effect.
A kind of anti-inflammation Chinese medicine composition provided by the invention has the following advantages:
1, Chinese medicine composition antiinflammatory action of the present invention is obvious, and to rat paw edema due to Ovum Gallus domesticus album, after administration, rat toes swelling alleviates extremely significantly (P < 0.01); And the present invention compares with comparative example group, there is significant difference (P < 0.01 or P < 0.05), show that the present invention alleviates rat toes swelling effect and is significantly better than prior art.
2, the present invention all has inhibitory action in various degree to staphylococcus aureus, escherichia coli, bacillus pyocyaneus, Bacillus proteus; Minimal inhibitory concentration of the present invention is all lower than comparative example, and result shows, Chinese medicine composition antibacterial action provided by the invention is better than prior art.
3, the data of clinical trial routine urinalysis and urinary system antibacterial removing situation show, the present invention is better to the therapeutic effect of urinary system infection, and antiinflammatory of the present invention, antibacterial effective percentage reach respectively 93.24% and 84.46%, and therapeutic effect is significantly better than matched group JIESHITONG PIAN (P<0.05).
The specific embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
If no special instructions, the concentration of alcohol in the present invention is percent by volume.
Embodiment 1:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 0.1% sodium bicarbonate solution extracts 2 times, each sodium bicarbonate solution that adds respectively 20 times, 15 times amounts of medical material gross weight, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 2 times, add respectively the water of 12 times, 10 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 2:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 1% sodium bicarbonate solution extracts 3 times, each sodium bicarbonate solution that adds respectively 20 times, 15 times, 10 times amounts of medical material gross weight, each extraction time is respectively 4 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 3 times, add respectively the water of 12 times, 10 times, 6 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours, 1 hour at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 3:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 0.5% sodium bicarbonate solution extracts 3 times, each sodium bicarbonate solution that adds respectively 20 times, 15 times, 10 times amounts of medical material gross weight, each extraction time is respectively 3 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 3 times, add respectively the water of 10 times, 8 times, 8 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 4:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 5% sodium bicarbonate solution extracts 2 times, each sodium bicarbonate solution that adds respectively 20 times, 10 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 2 times, add respectively the water of 10 times, 10 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 5:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 2% sodium bicarbonate solution extracts 3 times, each sodium bicarbonate solution that adds respectively 20 times, 15 times, 15 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 2 times, add respectively the water of 10 times, 8 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 6:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be that 0.1% sodium carbonate liquor extracts 3 times, each sodium carbonate liquor that adds respectively 20 times, 15 times, 15 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 2 times, add respectively the water of 10 times, 8 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 7:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis to add quality-percent by volume be 0.05% sodium hydroxide solution extraction 2 times, each sodium hydroxide solution that adds respectively 15 times, 8 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water are 2 times, add respectively the water of 10 times, 10 times amounts of three taste Chinese medicine gross weights at every turn, decoct respectively 3 hours, 2 hours at every turn, and collecting decoction, filters, and filtrate is condensed into fluid extract;
4) by step 2), 3) two kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 8: capsule
1, form: the fluid extract of embodiment 1 gained, be dried to dried cream powder, obtain 292g, add starch 63g, magnesium stearate 5.6g, micropowder silica gel 5.6g.
2, preparation method: the method with reference to conventional Chinese medicine preparation is made capsule.
Embodiment 9: tablet
1, form: the fluid extract of embodiment 2 gained, be dried to dried cream powder, obtain 318g, add microcrystalline Cellulose 103g, pregelatinized Starch 8.8g, micropowder silica gel 5.7g.
2, preparation method: the method with reference to conventional Chinese medicine preparation is made tablet.
Embodiment 10: granule
1, form: the fluid extract of embodiment 3 gained, be dried to dried cream powder, obtain 315g, add sucrose 1310g, pregelatinized Starch 156g.
2, preparation method: with reference to the method granulation agent of conventional Chinese medicine preparation.
Embodiment 11: oral liquid
1, form: embodiment 4 gained fluid extract 4.6L (1.050～1.065/30 ℃ of relative densities), sucrose 550g, monosodium glutamate 8.7g, sodium benzoate 5.4g.
2, preparation method: fluid extract is heated to more than 80 ℃, then add sucrose and antiseptic, embedding, sterilizing, makes oral liquid.
Embodiment 12: syrup
1, form: embodiment 5 gained fluid extract 9.0L (1.015～1.035/30 ℃ of relative densities), sucrose 8.2kg, Mel 1.0kg, sodium benzoate 22g.
2, preparation method: the method with reference to conventional Chinese medicine preparation is made syrup.
Comparative example 1:
With reference to the 13rd disclosed JIESHITONG PIAN methods of extraction and preparation of < < the Sanitation Ministry medicine standard > >, specific as follows:
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, get Poria 96g and are ground into fine powder, and seven tastes such as residue Poria and all the other Stigma Maydis decoct with water secondary, and each 3 hours, collecting decoction, filter, filtrate is condensed into the clear paste that relative density is 1.19 (90 ℃), adds Poria fine powder, mixes, dry, pulverize into fine powder, sieve, obtain.
Comparative example 2: full water extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, extracting in water 3 times adds the water of 10 times of amounts of medical material gross weight at every turn, each decoction 3 hours, collecting decoction, filters the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.10, adding concentration and be 95% ethanol makes to reach 60% containing alcohol amount, standing 12 hours, filter the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.15, be dried to dried cream powder, obtain.
Comparative example 3: full alcohol extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, use ethanol extraction 3 times, add 10 times of amount 80% ethanol of medical material gross weight at every turn, extract 2 hours at every turn, and merge extractive liquid,, filters, and the clear paste that when filtrate is concentrated into 60 ℃, relative density is 1.15, is dried to dried cream powder, obtains.
Experimental example 1: Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, the comparison of Herba Plantaginis extracting method
With different extracting method, Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis are extracted, by experiment the antibacterial action of more different extracts.
1 extracts solvent: 0.5% sodium bicarbonate solution, 30% alcoholic solution, water.
2 extracting method: Herba Desmodii Styracifolii 456g, Folium Pyrrosiae 228g, Herba lygodii 165g, Herba Abri 228g, Herba Plantaginis 165g, adding above-mentioned Different solution extracts respectively 3 times, each solvent that adds respectively 12 times, 10 times, 10 times amounts of three taste medical material gross weights, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
3 antibacterial effect test methods: paper disk method extracorporeal bacteria inhibitor test
4 analysis results are in Table 1
The In Vitro Bacteriostasis of table 1 Different Extraction Method
Table 1 result shows: 0.5% the sodium bicarbonate solution of take is the extract that solvent extracts gained, and the minimal inhibitory concentration of staphylococcus aureus, escherichia coli, bacillus pyocyaneus, Bacillus proteus is minimum, and 30% ethanol experimental group and water experimental group fungistatic effect are poor.
Experimental example 2: Rhizoma Imperatae, Poria, Stigma Maydis three taste medicinal material extract method comparisons
With different extracting method, Rhizoma Imperatae, Poria, Stigma Maydis three taste medical materials are extracted, by the antiinflammatory effect of the more different extracts of animal effect experiment.
1 extracts solvent: 30% alcoholic solution, 70% alcoholic solution, water, 0.1% sodium bicarbonate solution.
2 extracting method: Rhizoma Imperatae, Poria, Stigma Maydis three taste medical materials add Different solution and extract 3 times, each solvent that adds respectively 12 times, 10 times, 8 times of three taste medical material gross weights, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
3 antiinflammatory test methods: Ovum Gallus domesticus album is caused to the impact of rat toes swelling.
4 analysis results are in Table 2
The antiinflammatory effect that table 2 Different Extraction Method is extract obtained
Note: with the comparison of blank group, * P<0.05, * * P<0.01; With the comparison of water extraction experimental group, #P<0.05, ##P < 0.01.
Note: with the comparison of water extraction experimental group, * P<0.05.
Table 2 result shows: the water of take extracts the extract of gained as solvent, to rat paw edema due to Ovum Gallus domesticus album, alleviate the highest, antiphlogistic effects is best, compares and has utmost point significant difference (P<0.01) with 30% ethanol experimental group, 70% ethanol experimental group, 0.1% sodium bicarbonate solution experimental group.
Experimental example 3: the comparison of Chinese medicine composition extracting method
1 sample grouping
Embodiment 1-7, comparative example 1-3 make respectively dried cream powder, grouping.
2 contrast projects
General flavone content: use colorimetric method for determining general flavone content.
3 experimental results, in Table 3
The extraction effect of table 3 Different Extraction Method to medical material
| Group | Total flavones (mgg-1) |
| Embodiment 1 | 74..567±1.77 |
| Embodiment 2 | 73.547±1.84 |
| Embodiment 3 | 74.510±1.79 |
| Embodiment 4 | 73.058±1.76 |
| Embodiment 5 | 74.987±1.71 |
| Embodiment 6 | 74.247±1.79 |
| Embodiment 7 | 73.687±1.81 |
| Comparative example 1 | 56.371±1.73 |
| Comparative example 2 | 59.864±1.70 |
| Comparative example 3 | 58.536±1.85 |
Table 3 result shows: the general flavone content of embodiment of the present invention 1-7 is better than comparative example 1-3.Experimental result prompting total flavones extractive technique of the present invention compared with prior art, is extracted more complete.
The experiment of anti-inflammation drug effect
One, paper disk method extracorporeal bacteria inhibitor test
1, experiment material
(1) bacterial strain
Staphylococcus aureus (CICC23656), escherichia coli (ACCC10141), bacillus pyocyaneus, common degeneration bacillus (CMCC (B) 49027).
(2) medicine and reagent
Embodiment of the present invention 1-7, comparative example 1-3 medicine (crude drug amount 2.5g/mL medicinal liquid), standard filter paper; Carnis Bovis seu Bubali cream, peptone, agar, distilled water are mixed with nutrient broth, and sterilizing is standby.(3) instrument
High-pressure sterilizing pot (three Shens, Shanghai), biochemical cultivation case (Shanghai Yao Shi instrument), CJ-840 aseptic working platform (Purifying Equipment Co., Ltd., Suzhou), glass dish (autoclaving)
2, experimental technique
Each bacterial strain is 37 ℃ of cultivation 18～24h of constant temperature in suitable culture medium, rear with normal saline dilution standby (approximately 10
6/ mL).Each trial drug is diluted to the medicinal liquid that Concentraton gradient is 500,250,125,62.5,31.2,15.6,7.8mg/mL, and the standard filter paper of the sterilizing of learning from else's experience (diameter 6mm), dips respectively each test group medicinal liquid.On superclean bench, the beef peptone culture medium after sterilizing is poured in culture dish, every ware 15mL, places cooling.With aseptic pipet, get 0.1mL antibacterial liquid and be added in plate and evenly coat behind flat board (internal diameter 90mm) surface, the scraps of paper that speckle with medicinal liquid are put in plate, each culture dish is put 3 filter paper.Antibacterial is cultivated after 18～24h in 37 ℃, observed result, and the first water concentration that has no inhibition zone of take is minimal inhibitory concentration (MIC).
3, experimental result
The In Vitro Bacteriostasis of each test group medicine of table 4
The demonstration of table 4 result, embodiment 1-7 and comparative example 1-3Dui Jin Portugal bacterium, escherichia coli, bacillus pyocyaneus, Bacillus proteus all have inhibitory action in various degree; Embodiment 1 Dui Jin Portugal bacterium, escherichia coli, Bacillus proteus MIC are all less than comparative example 1-3,2 pairs of escherichia coli of embodiment, the golden bacterium MIC of Portugal are all less than comparative example 1-3, embodiment 3 Dui Jin Portugal bacterium, Bacillus proteus MIC are all less than comparative example 1-3, embodiment 4 Dui Jin Portugal bacterium, bacillus pyocyaneus MIC are all less than comparative example 1-3, embodiment 5 Dui Jin Portugal bacterium, bacillus pyocyaneus MIC are all less than comparative example 1-3, the embodiment 6 bacterium MIC of Dui Jin Portugal are all less than comparative example 1-3, and 7 couples of bacillus pyocyaneus MIC of embodiment are all less than comparative example 1-3.
Result shows, Chinese medicine composition antibacterial action provided by the invention is better than prior art.
Two, Ovum Gallus domesticus album is caused the impact of rat toes swelling
1, experiment material
(1) animal
SD rat, male, body weight 200 ± 20g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
(2) medicine and instrument
Embodiment of the present invention 1-7, comparative example 1-3,0.9% normal saline (Pharmaceutical Group Co.,Ltd of Nanning Baihui), dexamethasone (Zhejiang Province XianJu Pharmacy stock Co., Ltd), Ovum Gallus domesticus album, flexible plastic chi.
2, experiment grouping
Select 120 of healthy SD rats, female, be divided at random 12 groups, 10 every group:
Blank group: gavage equal-volume normal saline;
Test group: give embodiment 1-7, comparative example 1-3 medicine;
Positive controls: gavage equal-volume dexamethasone medicinal liquid.
3, experimental technique
Each organizes rat oral gavage administration, and embodiment 1-7 and comparative example 1-3 group dosage are all by crude drug amount 3.1g/kg, and positive drug group gives the dexamethasone solution of dosage 20mg/kg, and blank group gives equal-volume normal saline, and 1 time/d, continuous 6 days.After last administration 30min at every Mus right hind vola far-end inserting needle near ankle joint, subcutaneous injection 10% fresh albumen solution 0.1mL.Measure respectively cause scorching before and cause scorching after 6h measure the girth (rat right hind leg is stretching, get the girth of rat ankle joint with flexible plastic dipstick metering, double, average) of rat ankle joint.Calculate as follows paw swelling:
Swelling (%)=(cause scorching after ankle joint girth-cause scorching before ankle joint girth)/cause scorching before ankle joint girth * 100%.
4, experimental result
The impact of each trial drug of table 5 on rat paw edema due to Ovum Gallus domesticus album
Note: with the comparison of blank group, * P < 0.05, * * P < 0.01; With 1 group of comparison of comparative example,
△p < 0.05,
△ △p < 0.01; With 2 groups of comparisons of comparative example,
▲p < 0.05,
▲ ▲p < 0.01; With 3 groups of comparisons of comparative example,
◆p < 0.05,
◆ ◆p < 0.01.
Table 5 result shows, compares with blank group, and embodiment 1-7 group, comparative example 1-3 group rat toes swelling all obviously alleviate (P < 0.01, P < 0.05); And embodiment 1-7 group is compared with comparative example 1-3 group, there is significant difference (P < 0.01, P < 0.05).
Result shows: Chinese medicine composition antiinflammatory action provided by the invention is better than prior art.
Clinical trial:
The curative effect of anti-inflammatory, antibacterial of the present invention is carried out to clinical observation, by antiinflammatory with to the removing status analysis of urinary system pathogenic bacterium anti-inflammation effect of the present invention.
1 diagnosis basis
1.1 Urinary system inflammation
1.1.1 symptom
Disease is seen shiver with cold, fever, urgent micturition, frequent micturition, dysurea, lumbago, unable, hematuria, pyuria etc., chronic phase inflammation or inflammation repeatedly show effect and can occur edema, hypertension, albuminuria etc.
1.1.2 routine urianlysis
Specific gravity of urine increases or reduction, urine protein positive, glucose in urine positive, and microscopy finds that erythrocyte is greater than 3/HP, finds leukocyte and epithelial cell, cast etc.
1.1.3 imaging examination
By plain abdominal radiograph, urography, MRI etc., can further determine inflammation part and degree.
1.2 urinary system infection
1.2.1 the urinary tract infection symptom such as frequent micturition, urgent micturition, dysurea, or have that lower abdomen is touched a tender spot, kidney district kowtows pain, companion or the heat that do not occur together, and there is the one of following situations:
1. urine examination male leukocyte >=5/high power field, female WBC >=10/high power field, plugs in catheter patient and should cultivate in conjunction with urine.
2. clinicist is diagnosed as urinary tract infection, or antibacterial therapy effectively and the urinary tract infection of assert.
1.2.2 etiological diagnosis
On clinical diagnosis basis, meet the one of following situations:
1. CCMS or urethral catheterization are left and taken urine (non-indwelling catheter) and are cultivated gram-positive cocci bacterium number >=104cfu/ml, gram negative bacilli bacterium number >=105cfu/ml.
2. on pubic symphysis, punctio vesicae is left and taken urine culture of bacteria bacterium number >=103cfu/ml.
3. freshly voided urine specimen is through centrifugal, and application phase-contrast microscopy (1X400) has the half visual field to see antibacterial in 30 visuals field.
4. ABU disease: although patient is asymptomatic, but there are endoscopy or indwelling catheter history in recent (1 week), urine is cultivated gram-positive cocci concentration >=104city/ml, gram negative bacilli concentration >=105cfu/ml, should be considered as urinary system infection.
2 case selections
20～70 years old age, be ready to accept 2 weeks therapists, signature enters clinical research Informed Consent Form person.
3 medicines
Experimental group: embodiment 3
Matched group: JIESHITONG PIAN, Guangxi Wuzhou Pharmaceutical (group) limited company
4 curative effect index
4.1 urine routine test
Cure: clinical symptoms and sign all disappear; Urine routine test total negative;
Take a turn for the better: clinical symptom disappearance; Urine routine test has a little erythrocyte;
Invalid: symptom is not improved, urine routine test has erythrocyte, urine protein, epithelial cell;
4.2 bacteriology's curative effects check
Remove: treatment finishes rear original pathogenic bacterium and disappears;
Part is removed: in original various pathogens, have to be severally eliminated;
Do not remove: treatment finishes rear original pathogenic bacterium and still exists.
5 clinical grouping and Therapeutic Method
Enter to organize the routine patient of case 296, male 148 examples, female's 148 examples; Average approximately 46 years old; All patients are all through color ultrasound or the inspection of abdomen plain film, and routine urinalysis etc. are diagnosed as urinary system calculus companion antibacterial and infect, and case is divided into two groups at random.
Therapeutic Method:
Experimental group: the embodiment of the present invention 5, is equivalent to crude drug 10g each serving consumption, three times on the one.
Matched group: JIESHITONG PIAN, each 1.5g, three times on the one.
Experimental group and matched group, taking medicine simultaneously, strengthen amount of drinking water every day, and average daily drink amount is 3000～5000ml, takes exercises more, take jumping exercise or going upstairs motion as main, and both hands are in dorsal part intersection knocking back lumbar part.
6 results
In experimental example group case, most of patient takes after the embodiment of the present invention 3, and Urinary system inflammation disappears or alleviates, and urinary system pathogenic bacterium are removed or part is removed.
Table 6: routine urianlysis result after treatment
Note: experimental group and matched group comparison, * P < 0.05.
Table 7: bacteriology checking result after treatment
Note: experimental group and matched group comparison, * P < 0.05.
Interpretation of result: the data that above-mentioned routine urinalysis and urinary system antibacterial are removed situation show, experimental group and matched group are still good to the therapeutic effect of urinary system infection, but experimental group is significantly better than matched group (P<0.05), its antiinflammatory, antibacterial effective percentage reach respectively 93.24% and 84.46%.Medicine anti-inflammatory, antibacterial of the present invention is respond well, is significantly better than JIESHITONG PIAN.
Model case:
1. Song, man, 30 years old, peasant.This patient is in left lower abdomen paroxysmal pain on May 10th, 2007, because increasing the weight of gradually and carrying out hospital admission with hematuria.Ultrasonic demonstration: left side ureter radiopaque calculus, routine urianlysis, cloudy urine, and adularescent precipitate, erythrocyte +++, albumen ++, leukocyte ++, escherichia coli are positive.Give the embodiment of the present invention 5 capsules, 3 times on the 1st, each 5.And coordinate jump action and day drink boiled water 4000ml left and right, and after taking medicine 3 days, all diseases alleviate, and urine is normal, discharges 1 piece, calculus during the 7th day urine, diameter 0.2cm, within the 8th day, check routine urinalysis is normal, and escherichia coli check negative, ultrasonic check, patient is without left renal pelvis hydrops, and left ureter calculus disappears.
2. patient, the Uygur nationality, male, 20 years old, stuttering urination difficulty, urine stream interrupts, hematuria, urine urgency-frequency, and have the soreness of waist, waist is swollen, on pubic symphysis, dull pain is more than 1 year.When the training of being admitted to hospital is had a medical check-up, clinical principium is diagnosed as vesical calculus.Through routine urianlysis: erythrocyte +++, albumen ++, leukocyte +++, escherichia coli are positive.Ultrasound diagnosis prompting: 2 of vesical calculuses, size is about 1.6cm.Give the embodiment of the present invention 11 oral liquids, 3 times on the 1st, each 2 bottles.Further consultation after two weeks, patient's lumbago slows down, and urine is profit freely, exclusive segment rubble, spiritual amelioration, check routine urinalysis is normal, and B ultrasonic is detected prompting bladder and is had no calculus, 2 pieces, urine output my humble opinion calculus, maximum gauge is 0.3cm, urine escherichia coli are cultivated and show feminine gender.
Although, above used general explanation, the specific embodiment and test, the present invention is described in detail, on basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. an anti-inflammation Chinese medicine composition, is characterized in that the preparation method of its active component comprises the following steps:
1) get the medical material of following weight portion: 456 parts of Herba Desmodii Styracifolii, 302 parts of Stigma Maydis, 228 parts of Folium Pyrrosiaes, 228 parts of Herba Abris, 228 parts, Poria, 165 parts of Herba Plantaginiss, 165 parts of Herba lygodiis, 228 parts of Rhizoma Imperataes;
2) Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis are used alkaline aqueous solution to extract for extracting solvent, and extract is made fluid extract;
3) Rhizoma Imperatae, Poria, Stigma Maydis extracting in water, extract is made fluid extract;
4) by step 2), 3) all fluid extracts of gained two mix, obtain.
2. Chinese medicine composition according to claim 1, is characterized in that, described step 2) neutral and alkali aqueous solution is: the sodium bicarbonate solution that quality-percent by volume is 0.1-5%.
3. according to the Chinese medicine composition described in claim 1 or 2 any one, it is characterized in that described step 2) neutral and alkali aqueous solution is: the sodium bicarbonate solution that quality-percent by volume is 0.5-1%.
4. Chinese medicine composition according to claim 1, is characterized in that, described step 2) in the extracting method of Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis be:
Herba Desmodii Styracifolii, Folium Pyrrosiae, Herba lygodii, Herba Abri, Herba Plantaginis add alkaline aqueous solution extracts 2-3 time, adds the alkaline aqueous solution that medical material gross weight 10-20 doubly measures at every turn, and each extraction time is 1-4 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.
5. Chinese medicine composition according to claim 1, it is characterized in that, the water extraction method of described step 3) is: Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2-3 time, add the water that three taste Chinese medicine gross weight 6-12 doubly measure at every turn, each 1-3 hour that decocts, collecting decoction, filters, and filtrate is condensed into fluid extract.
6. according to the Chinese medicine composition described in claim 1 or 5 any one, it is characterized in that, the water extraction method of described step 3) is: Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2-3 time, add the water that three taste Chinese medicine gross weight 8-10 doubly measure at every turn, each 2-3 hour that decocts, collecting decoction, filters, and filtrate is condensed into fluid extract.
7. Chinese medicine composition according to claim 6, is characterized in that, the water extraction method of described step 4) is: Rhizoma Imperatae, Poria, Stigma Maydis extracting in water 2 times, add the water that three taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct collecting decoction at every turn 3 hours, filter, filtrate is condensed into fluid extract.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104983944A (en) * | 2015-06-15 | 2015-10-21 | 山东大学 | Compound Chinese herbal medicine anti-microbial anti-viral agent and preparing method and application thereof |
| CN105055579A (en) * | 2015-09-11 | 2015-11-18 | 吉林大学 | Compound traditional Chinese medicine composition for treating urolithiasis and preparation method thereof |
| CN105395930A (en) * | 2015-11-30 | 2016-03-16 | 梁苡玮 | Antisepsis and anti-inflammation drug for external use |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
-
2013
- 2013-12-30 CN CN201310744580.0A patent/CN103705773A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈锐: "结石通片临床应用解析", 《中国社区医师》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104983944A (en) * | 2015-06-15 | 2015-10-21 | 山东大学 | Compound Chinese herbal medicine anti-microbial anti-viral agent and preparing method and application thereof |
| CN105055579A (en) * | 2015-09-11 | 2015-11-18 | 吉林大学 | Compound traditional Chinese medicine composition for treating urolithiasis and preparation method thereof |
| CN105055579B (en) * | 2015-09-11 | 2018-12-14 | 吉林大学 | A kind of compound traditional Chinese medicine composite and preparation method for treating urolithiasis |
| CN105395930A (en) * | 2015-11-30 | 2016-03-16 | 梁苡玮 | Antisepsis and anti-inflammation drug for external use |
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