CN103751583A - Traditional Chinese medicine composition for being diuretic and protecting liver and preparation method of composition - Google Patents
Traditional Chinese medicine composition for being diuretic and protecting liver and preparation method of composition Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for being diuretic and protecting liver and a preparation method of the composition. The preparation method of the traditional Chinese medicine composition disclosed by the invention mainly comprises the following steps of taking medicinal materials including desmodium styracifolium, corn stigma, pyrrosia lingua, abrus cantoniensis hance, poria cocos, plantain herb, lygodium japonicum sw and lalang grass rhizome, extracting abrus cantoniensis hance by using acid aqueous solution, after extracting corn stigma, plantain herb and lygodium japonicum sw by using high-concentration ethanol, mixing alcohol extraction decoction dregs of corn stigma, plantain herb and lygodium japonicum sw with desmodium styracifolium, pyrrosia lingua, lalang grass rhizome and poria cocos, carrying out aqueous extraction together, finally, mixing decoction through three extraction methods, and then, obtaining fluid extract. By means of animal pharmacological experiments and clinical experiments, the results prove that the diuretic and liver-protecting effect of the traditional Chinese medicine composition provided by the invention is prior to that in the prior art while treating lithiasis in the urinary system.
Description
Technical field
The present invention relates to treat the Chinese medicine composition of urinary system calculus, be specifically related to Chinese medicine composition that a kind of diuresis protects the liver and preparation method thereof.
Background technology
Urinary system calculus belongs to " sand Stranguria ", " stranguria caused by urinary stone " and " stranguria with blood " scope in traditional Chinese medical science gonorrhea, mainly comprises 4 kinds of pattern of syndrome:
(1) qi stagnation and blood stasis type: disease is seen waist dull pain, dull pain, the normal or stringy and tense pulse of arteries and veins, tongue is normal; Or urine is interrupted suddenly when excessive, sharp ache, the upper waist abdomen that connects, sandstone discharge after pain slow; Or waist, flank portion tugging pain be as strand, pain extending to the lower abdomen, outbreak again and again, pale complexion, cold sweat, vomiting and nausea during pain, companion's hematuria or urine yellow skin are red, dimly red tongue or have petechia, ecchymosis, stringy and tense pulse or slow puckery.
(2) damp heat downward flowing type: card is shown in fever with aversion to cold, lumbago, few abdomen is anxious full, and frequent micturition is short red, and while drowning, puckery pain unbearably, is drenched drop not well.Yellow and greasy fur, stringy and rolling pulse or sliding number.
(3) deficiency of the kidney yin type: calculus is stopped for a long time, pathogenic heat are strongly fragrant for a long time.Kidney-Yin loss, dizzy tinnitus, pain in the low back and legs, dribbling urination or not well, insomnia and dreamful sleep, time have low grade fever, shortness of breath and palpitation, dysphoria with feverish sensation in the chest palms and soles, night sweat, eye is dry or puckery, abdominal distention constipation, poor appetite, thready and rapid pulse, red tongue or few tongue.
(4) kidney yang deficiency syndrome: calculus is stayed for a long time, kidney yang deficiency, the acid of waist lower limb is heavy, lassitude, whole body is timid cold, and extremities being not warm or the lower part of the body often have creeping chill, frequent micturition or dysuria, frequent urination at night, complexion is white, a little less than deep-thready pulse, the white pale tongue of tongue.
The 13rd disclosed JIESHITONG PIAN of < < the Sanitation Ministry medicine standard > > discloses following information:
1) composition: Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperatae.
2) method for making: above eight tastes, to get Poria 96g and be ground into fine powder, seven tastes such as residue Poria and all the other Stigma Maydis decoct with water secondary, each 3 hours, collecting decoction, filtered, filtrate is condensed into the clear paste that relative density is 1.19-1.20 (90 ℃), adds Poria fine powder, mixes, dry, pulverize into fine powder, sieve, add appropriate amount of auxiliary materials, granule processed, mixes, be pressed into 1000, sugar coating, obtains.
3) function with cure mainly: clearing away heat-damp and promoting diuresis, Tonglin Paishi, pain easing and hemostasis.For urinary system infection, cystitis, oedema due to nephritis, lithangiuria, hematuria, drenches drop muddiness, urethra causalgia etc.
But in described ministry standard, there is no the proportional quantity of clearly each composition.
About the logical extracting method of calculus, there is following report:
Chinese patent application 01100318.9 is clear and definite, and the logical preparation method of calculus is: all pulverizing medicinal materials are nanoscale, microwave extracting, and concentrating under reduced pressure, dry.
Chinese patent application 200510032580.3 discloses the preparations such as the logical ball of calculus, drop pill, micropill, dispersible tablet, pellet capsule.The disclosed preparation method of this patent adopts part Poria to pulverize, residue Poria and all the other 7 taste medical material water extractions, or whole medical material water extraction.
Chinese patent application 200510047400.9 discloses a kind of Chinese medicine sugar free granule that is used for the treatment of lithangiuria and preparation method thereof.This patent raw material is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, and residue Poria and all the other 7 taste medical material water extractions, adopt low temperature drying method.
Chinese patent application 200910224023.x discloses the treatment pharmaceutical composition of diseases of urinary system and the detection method of component thereof.The raw material that this patent is used is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.The detection method of Herba Abri, chlorogenic acid in preparation is disclosed.
Chinese patent application 200910249899.x discloses the preparation method of pharmaceutical composition and the detection method of component thereof for the treatment of diseases of urinary system.The raw material that this patent is used is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.The detection method of Folium Pyrrosiae in preparation is disclosed.
Because the character of active component in eight taste medical materials is different, as preparation method in above-mentioned patent all adopts water extraction, can cause some active component to propose, or extraction ratio is low, affect the performance of drug effect.
Therefore, need to provide a kind of active component extraction ratio that can improve medicine, to improve the preparation method of pharmaceutical effectiveness.
Summary of the invention
The object of this invention is to provide the Chinese medicine composition that a kind of diuresis of determined curative effect protects the liver.
The Chinese medicine composition that a kind of diuresis provided by the invention protects the liver, the preparation method of its active component comprises the following steps:
1) get the medical material of following weight portion: 456 parts of Herba Desmodii Styracifolii, 302 parts of Stigma Maydis, 228 parts of Folium Pyrrosiaes, 228 parts of Herba Abris, 228 parts, Poria, 165 parts of Herba Plantaginiss, 165 parts of Herba lygodiis, 228 parts of Rhizoma Imperataes;
2) Herba Abri is used acidic aqueous solution to extract for extracting solvent, and extract is made fluid extract;
3) Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add the ethanol extraction that percent by volume is 60-80%, and extract is made fluid extract;
4) in step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water, and extract is made fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, obtain.
In the Chinese medicine composition step that described preparation diuresis protects the liver above:
Described step 2) in acidic aqueous solution be preferably: the citric acid solution that quality-percent by volume is 0.1-5%.
Described step 2) in acidic aqueous solution more preferably: the citric acid solution that quality-percent by volume is 0.5-1%.
Described step 2) in extracting method be preferably:
Herba Abri adds acid extraction with aqueous solution 2-3 time, adds the acidic aqueous solution that medical material gross weight 8-20 doubly measures at every turn, and each extraction time is 1-4 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.
Described step 3) is preferably:
Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add ethanol extraction 2-3 time that percent by volume is 60-80%, add two taste medical material gross weight 6-12 at every turn and doubly measure ethanol, and each extraction time is 1-5 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
Described step 3) is more preferably:
Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add ethanol extraction 2-3 time that percent by volume is 70-80%, add two taste medical material gross weight 8-10 at every turn and doubly measure ethanol, and each extraction time is 2-4 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
Described step 4) is preferably:
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water that seven taste Chinese medicine gross weight 6-12 doubly measure at every turn, decoct 1-3 hour, collecting decoction at every turn, filter, filtrate is condensed into fluid extract.
Described step 4) is more preferably:
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water that seven taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct 2-3 hour, collecting decoction at every turn, filter, filtrate is condensed into fluid extract.
Described step 4) is further preferably:
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, add the water that seven taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct 2-3 hour, collecting decoction at every turn, filter, filtrate is condensed into fluid extract.
The present invention also provides the preparation containing above-mentioned Chinese medicine composition, and said preparation is made separately by Chinese medicine composition or is comprised of Chinese medicine composition and pharmaceutically acceptable carrier.
Described preparation is solid preparation or liquid preparation, and solid preparation is tablet, capsule or granule; Liquid preparation is mixture or oral liquid.
Described pharmaceutically acceptable carrier is one or more in lactose, starch, dextrin, mannitol, sucrose, hydroxypropyl methylcellulose, cross-linking sodium carboxymethyl cellulose, low-substituted hydroxypropyl cellulose, microcrystalline Cellulose, micropowder silica gel, pregelatinized Starch, sorbitol, magnesium stearate, Pulvis Talci, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sodium benzoate, potassium sorbate, sucrose or aspartame, monosodium glutamate, Mel, orange flavor.
The preparation method of Chinese medicinal composition preparation of the present invention, the method can adopt conventional formulation technique to be prepared as various common dosage forms.
For example:
Method 1: Chinese medicinal composition capsules agent of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add capsule to commonly use adjuvant, production method is prepared into the capsule of Chinese medicine composition of the present invention routinely.
Method 2: Chinese medicine composition tablet of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add tablet to commonly use adjuvant, production method is prepared into the tablet of Chinese medicine composition of the present invention routinely.
Method 3: Chinese medicinal composition granules of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add granule to commonly use adjuvant, production method is prepared into the granule of Chinese medicine composition of the present invention routinely.
Method 4: Chinese medicine composition mixture of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add mixture to commonly use adjuvant, production method is prepared into the mixture of Chinese medicine composition of the present invention routinely.
Method 5: Chinese medicine composition oral liquid of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add oral liquid to commonly use adjuvant, production method is prepared into the oral liquid of Chinese medicine composition of the present invention routinely.
Method 6: Chinese medicine composition syrup of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add syrup to commonly use adjuvant, production method is prepared into the syrup of Chinese medicine composition of the present invention routinely.
The application that the present invention also provides above-mentioned Chinese medicine composition or preparation to improve diuresis liver protective effect when preparation treatment urinary system calculus disease.
Urinary system calculus belongs to stranguria caused by urinary stone, sand Stranguria in traditional Chinese medical science gonorrhea, due to damp invasion of lower energizer, changes the scorching the moon that burns, and decocts urine, becomes sandstone, alluvial water channel, and be stranguria caused by urinary stone.Amass in next bladder qi dysfunction, it is unfavorable to urinate, very want can not, poverty-strickenly feel bad, pain extending to the lower abdomen; Be stranded in, affect the duty of kidney department urine, pent-up must not be let out down, and QI and blood is unsmooth, and stagnation of QI and blood may bring about pain is involved bladder, pudendum by kidney; It is hematuria that sandstone is hindered network.Depressed rage impairing the liver, irritability is lost in catharsis, and blood defluidization is smooth for a long time, venation block, or stagnated QI transforming into fire, gas fire stagnation is in the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, so that disturbance in functioning of QI, and becomes pouring.Therefore, treatment stranguria caused by urinary stone should focus on inducing diuresis for treating stranguria syndrome, heat-clearing and toxic substances removing.For the disease disease that causes pouring because of impairing the liver, notice that hepatoprotective protects the liver, from source, control further developing of the state of an illness.
Chinese medicine composition effective ingredient disclosed in this invention is to be prepared from by raw materials such as Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperataes.Inventor finds in preparation research process for many years, identical drug dose, different extraction processes are prepared the active component of gained, also can there is larger difference and emphasis in its pharmacological action, for this reason, applicant has carried out a series of technical study to Chinese medicine composition of the present invention in conjunction with pharmacological evaluation, sum up process for extracting of the present invention, and brought drug effect potentiation through the such improvement of experimental verification: Herba Abri acidic aqueous solution extracts, Stigma Maydis, Herba Plantaginis, after Herba lygodii three tastes extract with high concentration ethanol, Stigma Maydis, Herba Plantaginis, the alcohol extraction medicinal residues of Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria is water extraction together, when the fluid extract that finally merges three kinds of extracting method gained is used for the treatment of urinary system calculus, its antibacterial and anti-inflammation functions is far superior to the existing extractive technique of identical prescription, clinical efficacy shows, extract of the present invention compared with prior art, diuresis protects the liver curative effect, and there were significant differences, there is significance progress.
Inventor detects Chinese medicine composition of the present invention, finds:
1, the alkaloids component content take abrine as representative is very high, and compared with existing extraction process products obtained therefrom, both have significant difference, may be because specific examples of such components in Herba Abri is soluble in acidic aqueous solution, so extract more complete in acidic aqueous solution.
2, total flavones content compared with existing extraction process products obtained therefrom is higher, may be because the flavones ingredient of Stigma Maydis, Herba Plantaginis, Herba lygodii is soluble in high concentration alcoholic solution, so extract more complete in 60-80% ethanol.
Above-mentioned testing result has shown that in each component, the extraction of some effective ingredient is better than prior art from the side.Because every taste Chinese crude drug all contains many active component, at least not yet there is any a herb purely and simply to be analyzed and know all the components, different Chinese medicine combines while extracting with different extracting modes, due to influencing each other and acting between solvent, temperature and heterogeneity, can produce very complicated chemical reaction.Therefore, above-mentioned effective ingredient is a part for Chinese medicine extract active component, for the drug action impact of overall medicine, is still not clear.We can only contrast different extracting modes in the quality aspect certain therapeutic use by effect experiment at present, and the method that there is no judges that from the change indicator of part effective ingredient the curative effect of compound Chinese patent medicine changes.
We contrast different extracting modes in the curative effect aspect the liver protecting by " on the impact of mice jaundice liver damage " zoopery; By " to the diuresis of rat " zoopery, contrast the effect of different extracting modes at diuresis face.
Inventor has carried out a large amount of systematic research experiments to extracting method and the excellent effect of medicine of each logical medical material of calculus, by medical material is split as to many groups, extract with diverse ways, three groups of extractive technique schemes of the present invention have finally been obtained, make the medical material of same recipe, given play to that therapeutic purposes are clearer and more definite, the effect of more remarkable treatment effect.
The Chinese medicine composition that a kind of diuresis provided by the invention protects the liver has the following advantages:
1, Chinese medicine composition diuresis of the present invention is obvious, and compared with blank group, the present invention has extremely significant diuresis (P < 0.01) to rat; Compared with comparative example group, also there is utmost point significant difference (P < 0.01).
2, Chinese medicine composition hepatoprotective effect of the present invention is remarkable, and oral disposition α-α-naphthyl isothiocyanate (ANIT) induced mice ALT, AST, the rising of DBIL value have obvious inhibitory action, and compared with comparative example group, Liver Damage in Rats degree obviously alleviates.In addition, hepatic tissue pathology section microscopy result has also confirmed that Chinese medicine composition provided by the invention can alleviate stem cell degeneration, necrosis, hemorrhage, promotes liver cell regeneration, and liver is played to good protective effect
3, clinical test results shows: the present invention has some improvement to patient's liver function, and the improvement of the present invention to patient's liver function and diuresis effect be better than matched group, have significant difference (P<0.05), the effect that drug diuresis of the present invention protects the liver is better than prior art JIESHITONG PIAN.
The specific embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
If no special instructions, the concentration of alcohol in the present invention is percent by volume.
Embodiment 1:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add quality-percent by volume be that 0.1% citric acid solution extracts 2 times, each citric acid solution that adds respectively 20 times, 15 times amounts of medical material gross weight, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 60% ethanol extraction 2 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 12 times, 8 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 5 hours, 3 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, each water that adds respectively 12 times, 10 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 2:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add quality-percent by volume be that 1% citric acid solution extracts 3 times, each citric acid solution that adds respectively 20 times, 15 times, 8 times amounts of medical material gross weight, each extraction time is respectively 4 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 80% ethanol extraction 3 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 12 times, 8 times, 6 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 3 hours, 3 hours, 1 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 3 times, each water that adds respectively 12 times, 10 times, 6 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours, 1 hour at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 3:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add quality-percent by volume be that 0.5% citric acid solution extracts 3 times, each citric acid solution that adds respectively 20 times, 15 times, 10 times amounts of medical material gross weight, each extraction time is respectively 3 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 70% ethanol extraction 3 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 10 times, 8 times, 8 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 4 hours, 3 hours, 2 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 3 times, each water that adds respectively 10 times, 8 times, 8 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 4:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add quality-percent by volume be that 5% citric acid solution extracts 2 times, each citric acid solution that adds respectively 20 times, 10 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 80% ethanol extraction 2 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 10 times, 8 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, each water that adds respectively 10 times, 10 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 5:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add quality-percent by volume be that 0.1% citric acid solution extracts 3 times, each citric acid solution that adds respectively 20 times, 15 times, 15 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 80% ethanol extraction 2 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 10 times, 8 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, each water that adds respectively 10 times, 8 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 6:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add percent by volume be 1% acetic acid extraction 3 times, each acetum that adds respectively 20 times, 15 times, 10 times amounts of medical material gross weight, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid,, filter, filtrate is condensed into fluid extract;
3) to add percent by volume be 80% ethanol extraction 2 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 10 times, 10 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 3 hours, 2 hours, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, each water that adds respectively 10 times, 8 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 7:
1) get the medical material of following weight portion: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri to add percent by volume be that 0.1% dilute hydrochloric acid solution extracts 2 times, add respectively the dilute hydrochloric acid solution of 10 times, 8 times amounts of medical material gross weight at every turn, each extraction time is respectively 2 hours, 2 hours, merge extractive liquid, filters, filtrate is condensed into fluid extract;
3) to add percent by volume be 80% ethanol extraction 3 times for Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials, add respectively 10 times, 8 times, 8 times amount ethanol of two taste medical material gross weights at every turn, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract;
4) Stigma Maydis after ethanol extraction in step 3), Herba Plantaginis, Herba lygodii three taste medicinal residues merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, each water that adds respectively 10 times, 10 times amounts of seven taste Chinese medicine gross weights, decoct respectively 3 hours, 2 hours at every turn, collecting decoction, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, and obtain Chinese medicine composition.
Embodiment 8: capsule
1, composition: the fluid extract of embodiment 1 gained, be dried to dried cream powder, obtain 290g, add mannitol 63g, magnesium stearate 5.5g, micropowder silica gel 5.5g.
2, preparation method: the method with reference to conventional Chinese medicine preparation is made capsule.
Embodiment 9: tablet
1, composition: the 1. fluid extract of embodiment 2 gained, be dried to dried cream powder, obtain 295g, add lactose 100g, pregelatinized Starch 8.6g, Pulvis Talci 5.5g
2, preparation method: the method with reference to conventional Chinese medicine preparation is made tablet.
Embodiment 10: granule
1, composition: the fluid extract of embodiment 1 gained, be dried to dried cream powder, obtain 285g, add sucrose 1150g, low-substituted hydroxypropyl cellulose 130g.
2, preparation method: with reference to the method granulation agent of conventional Chinese medicine preparation.
Embodiment 11: oral liquid
1, composition: 1. embodiment 4 gained fluid extract 4.5L (1.050～1.065/30 ℃ of relative densities), sucrose 540g, potassium sorbate 5.4g.
2, preparation method: fluid extract is heated to more than 80 ℃, then add sucrose and antiseptic, embedding, sterilizing, makes oral liquid.
Embodiment 12: syrup
1, composition: embodiment 6 gained fluid extract 9.5L (1.015～1.035/30 ℃ of relative densities), sucrose 9.5kg, orange flavor 25g, potassium sorbate 23g.
2, preparation method: the method with reference to conventional Chinese medicine preparation is made syrup.
Comparative example 1:
With reference to the 13rd disclosed JIESHITONG PIAN methods of extraction and preparation of < < the Sanitation Ministry medicine standard > >, specific as follows:
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, get Poria 96g and are ground into fine powder, and seven tastes such as residue Poria and all the other Stigma Maydis decoct with water secondary, each 3 hours, collecting decoction, filters, and filtrate is condensed into the clear paste that relative density is 1.19 (90 ℃), add Poria fine powder, mix, dry, pulverize into fine powder, sieve, obtain.
Gained Chinese medicine composition dry product weight is 270.8kg.
Comparative example 2: full water extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, extracting in water 3 times adds the water of 10 times of amounts of medical material gross weight at every turn, each decoction 3 hours, collecting decoction, filters the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.10, adding concentration and be 95% ethanol makes to reach 60% containing alcohol amount, standing 12 hours, filter the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.15, be dried to dried cream powder, obtain.
Comparative example 3: full alcohol extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, use ethanol extraction 3 times, add 10 times of amount 80% ethanol of medical material gross weight at every turn, extract 2 hours at every turn, and merge extractive liquid,, filters, and the clear paste that when filtrate is concentrated into 60 ℃, relative density is 1.15, is dried to dried cream powder, obtains.
Experimental example 1: Herba Abri extracting method comparison
Herba Abri is extracted with different extracting method, by the liver protecting effect of the more different extracts of animal effect experiment.
1 extracts solvent: 0.5% citric acid solution, 30% alcoholic solution, water.
2 extracting method: take Herba Plantaginis by recipe quantity, adding above-mentioned Different solution extracts respectively 3 times, each solvent that adds respectively 12 times, 10 times, 10 times amounts of medical material gross weight, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid,, filters, and reclaims medicinal residues, the clear paste that when filtrate is concentrated into 80 ℃, relative density is 1.20, is dried to fluid extract.
3 hepatoprotective effect test methods: on the impact of mice jaundice liver damage
Hepatic injury mice serum ALT, the AST that 4 Different Extraction Method are induced ANIT and the active impact analysis of DBIL the results are shown in Table 1
Hepatic injury mice serum ALT, the AST of table 1ANIT induction and the activity of DBIL
Note: with Normal group comparison, * * P < 0.01; With model control group ratio,
☆p < 0.05,
☆ ☆p < 0.01; With 0.5% citric acid solution experimental group comparison,
#p<0.05,
##p < 0.01.
Table 1 result shows: the citric acid solution take 0.5% is the extract of solvent extraction gained, the inhibitory action that oral disposition α-α-naphthyl isothiocyanate (ANIT) induced mice ALT, AST, DBIL value raise is the most obvious, hepatoprotective effect is best, has significant difference compared with 30% ethanol experimental group, water experimental group.
Experimental example 2: Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medicinal material extract method comparisons
Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials are extracted with different extracting method, by the diuretic effect of the more different extracts of animal effect experiment.
1 extracts solvent: 30% alcoholic solution, 70% alcoholic solution, water.
2 extracting method: take Stigma Maydis, Herba Plantaginis, Herba lygodii by recipe quantity, adding Different solution extracts 3 times, each 12 times of medical material gross weights, solvent of 10 times, 8 times of adding respectively, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid,, filters, and reclaims medicinal residues, the clear paste that when filtrate is concentrated into 80 ℃, relative density is 1.10, is dried to fluid extract.
3 diuretic test methods: to the diuresis of rat.
4 analysis results are in Table 2
The diuresis of table 2 Different Extraction Method to rat
| Group | Extract solvent | Administration 5h urine amount (mL) |
| 1 | Blank group | 2.54±0.49 |
| 2 | 30% ethanol | 3.10±0.54* ## |
| 3 | 70% ethanol | 4.36±0.75** |
| 5 | Water | 3.64±0.65** # |
Note: with the comparison of blank group, * P<0.05, * * P<0.01; With 70% ethanol experimental group comparison,
#p<0.05,
##p < 0.01.
Table 2 result shows: take 70% ethanol as solvent, extract the extract of gained, and best to the diuresis of rat, there is significant difference compared with 30% ethanol experimental group, water experimental group.
Experimental example 3: the comparison of Chinese medicine composition extracting method
1 sample grouping
Embodiment 1-7, comparative example 1-3 make respectively dried cream powder, grouping.
2 contrast projects
Alkaloids content: the alkaloids effective ingredient with high effective liquid chromatography for measuring take abrine as contrast;
General flavone content: use colorimetric method for determining general flavone content.
3 experimental results, in Table 3
The extraction effect of table 3 Different Extraction Method to medical material
| Group | Abrine (mgg-1) | Total flavones (mgg-1) |
| Embodiment 1 | 0.733±1.60 | 88.623±1.73 |
| Embodiment 2 | 0.784±1.61 | 88.623±1.73 |
| Embodiment 3 | 0.821±1.57 | 91.875±1.73 |
| Embodiment 4 | 0.763±1.62 | 88.623±1.73 |
| Embodiment 5 | 0.724±1.63 | 87.878±1.73 |
| Embodiment 6 | 0.717±1.58 | 87.988±1.73 |
| Embodiment 7 | 0.722±1.60 | 88.261±1.73 |
| Comparative example 1 | 0.423±1.65 | 55.875±1.73 |
| Comparative example 2 | 0.473±1.63 | 58.963±1.80 |
| Comparative example 3 | 0.459±1.59 | 57.352±1.75 |
Table 3 result shows: alkaloids composition, the general flavone content of embodiment of the present invention 1-7 take abrine as representative is better than comparative example 1-3.Experimental result prompting the technology of the present invention extraction alkaloid component and total flavones compared with prior art, extract more complete.
Diuresis protects the liver effect experiment
The diuresis of 1 pair of rat
1.1 experiment material
1.1.1 animal
Healthy SPF level SD male rat, body weight 200 ± 20g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
1.1.2 medicine
Embodiment of the present invention 1-7, comparative example 1-3,0.9% normal saline (Guizhou Kelun Pharmaceutical Co., Ltd.), hydrochlorothiazide tablet (Shanghai Sine Pharmaceutical Co., Ltd.)
1.1.3 instrument
Metabolism of rat cage
1.2 experimental technique
1.2.1 preliminary election animal: SD rat, with normal saline gavage 2.5mL/100g, is collected twenty-four-hour urine liquid with metabolic cage, calculates urine amount/gavage amount and is greater than 40% for qualified.
1.2.2 get 120 of preliminary election rats, be divided at random 12 groups, be respectively blank group, hydrochlorothiazide group, embodiment of the present invention 1-7 group and comparative example 1-3 group, 10 every group.First fasting (the can't help water) 12h of laboratory animal, then respectively organizes rat by 2.5mL/100g body weight, with normal saline gavage, gives certain water load, and presses rat lower abdomen and make bladder emptying.Then respectively organize rat oral gavage administration, blank group to wait capacity physiologic saline for substitute.After administration, rat is put into metabolic cage, collect the urine amount of 5h.
1.3 experimental result
Table 4 distinct methods extract the diuresis of medicine to rat (
n=10)
Note: with the comparison of blank group, * P < 0.05, * * P < 0.01; With 1 group of comparison of comparative example,
△p < 0.05,
△ △p < 0.01; With 2 groups of comparisons of comparative example,
▲p < 0.05,
▲ ▲p < 0.01; With 3 groups of comparisons of comparative example,
◆p < 0.05,
◆ ◆p < 0.01.
The demonstration of table 4 result, compared with blank group, embodiment 1-7 group, comparative example 1-3 group have significant diuresis (P < 0.01, P < 0.05) to rat; And to the diuresis of rat, compared with comparative example 1-3 group, there is significant difference (P < 0.01, P < 0.05) in embodiment 1-7 group.
Result shows: the diuresis of Chinese medicine composition provided by the invention is better than prior art.
2 impacts on mice jaundice liver damage
2.1 experiment material
2.2.1 animal
Healthy SPF level Kunming mouse, male and female half and half, body weight 18～22g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
2.2.2 medicine and reagent
Embodiment of the present invention 1-7, comparative example 1-3,0.9% normal saline (Guizhou Kelun Pharmaceutical Co., Ltd.), α-α-naphthyl isothiocyanate (ANIT, AR), formaldehyde (AR); DBIL, ALT, AST measure test kit (Bioengineering Research Institute is built up in Nanjing); Hematoxylin-eosin solution (Foochow steps new).
2.2 experiment groupings
Get 120 of mices, be divided at random 12 groups: Normal group, model control group, embodiment 1-7 group, comparative example 1-3 group, 10 every group.
2.3 experimental technique
Normal group, model control group gavage normal saline, embodiment 1-7 and comparative example 1-3 group dosage, all by crude drug amount 4.5g/kg, every day 1 time, are total to administration 10 days.Before the 9th day gavage, for mice, again weigh, administration after 2 hours except normal group, all the other each group gavage ANIT olive oil solution (90mg/kg) modelings, modeling is plucked eyeball of mouse and is got blood and (get blood fasting in first 12 hours after 46 hours, but can't help water), centrifugal 15 minutes of 3000 turn/min, separation of serum, full automatic biochemical apparatus is surveyed DBIL, ALT, AST.After getting blood, get about right lobe of liver, more than being put in the fixing 24h of 10% formalin, specimens paraffin embedding slices, HE dyeing, tissues observed degree of impairment under optical microscope.
2.4 experimental result
Hepatic injury mice serum ALT, the AST of table 5ANIT induction and the activity of DBIL (
n=10)
Note: with Normal group comparison, * P < 0.05, * * P < 0.01; With model control group comparison,
☆p < 0.05,
☆ ☆p < 0.01; With 1 group of comparison of comparative example,
△p < 0.05,
△ △p < 0.01; With 2 groups of comparisons of comparative example,
▲p < 0.05,
▲ ▲p < 0.01; With 3 groups of comparisons of comparative example,
◆p < 0.05,
◆ ◆p < 0.01.
2.5 conclusion
2.5.1 the specific activity of mice serum ALT, AST and DBIL
After the disposable oral ANIT of model mice, Serum ALT, AST, DBIL raise rapidly, and with normal group comparison, significant difference (P < 0.01), illustrates modeling success.Embodiment 1-7 group and comparative example 1-3 group ALT, AST, DBIL are all starkly lower than model group, relatively there is significant difference (p < 0.05 with model group, p < 0.01), prompting embodiment 1-7 group and comparative example 1-3 group oral disposition α-α-naphthyl isothiocyanate (ANIT) induced mice ALT, AST, the rising of DBIL value have obvious inhibitory action.
2.5.2 liver histopathology is observed
Om observation: normal group mouse liver cell, portal area, bile duct district no abnormality seen, model group mouse liver cell pathological changes is the most serious, most of swelling of liver cell, endochylema is balloon sample and becomes, having a liking for sour sample becomes, visible obvious point-like, lamellar, focal necrosis, residue after a little cytolysis is seen in necrotic area, bile duct epithelial cell swelling, necrosis, visible non-viable non-apoptotic cell chip in tube chamber, bile duct proliferation, large amount lymphocyte, neutrophil cell, mononuclear cell, macrophages infiltration are seen by portal area, bile duct district, have a liking for as seen sour sample corpusculum and occur; Sinus hepaticus obviously expands, visible red cell accumulation.Treatment group also has the hepatic injury pathological changes such as hepatocyte destruction, pericholangitis, inflammatory cell infiltration in various degree, but hepatocellular degeneration, downright bad light, be a kitchen range shape and slight piecemeal necrosis, in portal area, lobule, cell infiltration obviously reduces, leaflet structure is complete, hepatic injury degree obviously alleviates compared with model group, and embodiment 1-7 group is more remarkable compared with comparative example 1-3 group effect.In addition, hepatic tissue pathology section microscopy result has also confirmed that Chinese medicine composition provided by the invention can alleviate stem cell degeneration, necrosis, hemorrhage, promotes liver cell regeneration, and liver is played to good protective effect.
Result shows, Chinese medicine composition provided by the invention has protective effect to hepar damnification, and effect is better than prior art.
Clinical trial:
The curative effect that diuresis of the present invention is protected the liver is carried out clinical observation, and the urine quantitative change to Patients with Urinary Stone before and after taking medicine by contrast and the situation of Hepatic function improvement, analyze the effect that diuresis of the present invention protects the liver.
1 diagnosis basis
1.1 hypourocrinia: discharge in physiological situation, collect patient 24h urine preparing, adult's normal urine amount is 1000-2000mL/24h.24h urine amount is less than 400mL or urine amount per hour, and to be less than 17mL be hypourocrinia.
1.2 abnormal liver function
1.2.1 protein metabolism functional check: total serum protein, albumin, globulin, albumin and globulin ratio A/G etc. are higher or lower than clinical normal value.
1.2.2 bilirubin metabolism functional check: detect serum total bilirubin, bilirubin direct and unconjugated bilirubin.
1.2.3 serum enzyme inspection: serum alanine aminotransferase (ALT) normal reference value: colorimetry is 5-25U(karmen unit), performance rate method <40U/L(37 ℃); Serum aspartate amino transferase (AST) normal reference value 8-12U; Serum alkaline phosphatase (ALP) normal reference value: colorimetry 3-13 King unit, performance rate method <240U/L.
2 case selections
20～70 years old age, be ready to accept 2 weeks therapists, signature enters clinical research Informed Consent Form person.
3 medicines
Experimental group: embodiment 3
Matched group: JIESHITONG PIAN, Guangxi Wuzhou Pharmaceutical (group) limited company
4 curative effect index
4.1 urine quantitative changes
Criterion of therapeutical effect: effective: urine amount 24h1500-2500mL; Effective: urine amount increases; Invalid: urine amount is without significant change or minimizing person.
4.2 liver function test
Effective: clinical symptoms is clearly better, hepatosplenomegaly dwindle or stablize constant, liver function test ALT, it is normal that TB recovers;
Effective: clinical symptoms takes a turn for the better or stablizes constantly, and hepatosplenomegaly is stablized constant, liver function test ALT, AST or (with) more than TB reduces >50% before;
Invalid: without changing, not reach above standard or disease progression person.
5 clinical grouping and Therapeutic Method
Enter to organize the routine patient of case 342, male 180 examples, female's 162 examples; Average approximately 45 years old; All there is abnormal liver function through making a definite diagnosis in all trouble urinary system calculus, case is divided into two groups at random.
Therapeutic Method:
Experimental group: the embodiment of the present invention 5, is equivalent to crude drug 10g each serving consumption, three times on the one.
Matched group: JIESHITONG PIAN, each 1.5g, three times on the one.
Experimental group and matched group, taking medicine simultaneously, strengthen amount of drinking water every day, and average daily drink amount is 3000～5000ml, takes exercises more, take jumping exercise or going upstairs, move as main, and both hands are in dorsal part intersection knocking back lumbar part.
6 results
In experimental example group case, most of patient takes after the embodiment of the present invention 3, and urine amount obviously increases, and through liver function test, liver function recovery situation is effective or effective.
Table 6: the diuretic effect after treatment
Note: experimental group and matched group comparison, * P < 0.05.
Table 7: liver function test effect after treatment
Note: experimental group and matched group comparison, * P < 0.05.
Interpretation of result: the above results shows, experimental group and matched group all have some improvement to patient's liver function, and the improvement of experimental group to patient's liver function and diuresis effect be better than matched group, there is significant difference (P<0.05).The effect that drug diuresis of the present invention protects the liver is better than JIESHITONG PIAN.
Model case:
Profit certain, man, 54 years old, card was shown in waist abdomen pain, time be angor, body of the tongue is dim, have petechia, greasy fur, stringy and thready pulse is puckery.B ultrasonic prompting: left hydronephrosis (on a small quantity), left ureter stage casing calculus (1.6cm × 1.1cm), patient's readme has fatty liver, and liver function test finds that its AST, ALT and TB value are respectively 79,83 and 62.Card belongs to damp invasion of lower energizer, qi depression to blood stasis.Give the embodiment of the present invention 3 granules, 3 times on the 1st, each 1 bag, 4000mL drinks water every day; Do again jumping exercise.Treat after 3 days, controlled micturition obviously increases, and after treatment 13d, discharges the calculus of one piece of 1.7cm × 1.3cm.Check B ultrasonic shows: hydronephrosis and calculus shade disappear; Liver function test finds that AST, ALT and TB significantly reduce, and all recovers normal.
2. Wang, male, 65 years old, before more than 10 years, without obvious inducement, there is bilateral waist distending pain discomfort, paroxysmal outbreak, without fear of cold, heating, feel sick, vomiting, frequent micturition, urgent micturition, dysurea, hematuria, pyuria etc.Slightly heavy because of base disease before day, there is suddenly oliguria, in hospital diagnosis, be two renal calculuss, and hydronephrosis, and seek medical advice because morbidity is before this, but buy medicine to pharmacy, treat voluntarily, cause hepatic injury, liver function test shows that ALT, AST are all significantly higher than normally, reach respectively 201,79.The embodiment of the present invention 5 capsules, 3 times on the 1st, each 5.And coordinate jump action and day drink boiled water 4000ml left and right, after 2 weeks, discharge the calculus of one piece of 0.7cm × 0.3cm.Continue to take 1 week, every day, amount of drinking water was 4000ml left and right, further consultation again after a week: patient's private prosecution is without uncomfortable, and check routine urinalysis, B ultrasonic are detected equal to abnormal; Liver function test shows that ALT, AST value are respectively 83,47, and liver function recovery is good.
Although, above used general explanation, the specific embodiment and test, the present invention is described in detail, on basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. the Chinese medicine composition that diuresis protects the liver, is characterized in that the preparation method of its active component comprises the following steps:
1) get the medical material of following weight portion: 456 parts of Herba Desmodii Styracifolii, 302 parts of Stigma Maydis, 228 parts of Folium Pyrrosiaes, 228 parts of Herba Abris, 228 parts, Poria, 165 parts of Herba Plantaginiss, 165 parts of Herba lygodiis, 228 parts of Rhizoma Imperataes;
2) Herba Abri is used acidic aqueous solution to extract for extracting solvent, and extract is made fluid extract;
3) Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add the ethanol extraction that percent by volume is 60-80%, and extract is made fluid extract;
4) in step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water, and extract is made fluid extract;
5) by step 2), 3), 4) three kinds of fluid extracts of gained mix, obtain.
2. Chinese medicine composition according to claim 1, is characterized in that step 2) described acidic aqueous solution is: the citric acid solution that quality-percent by volume is 0.1-5%.
3. Chinese medicine composition according to claim 2, is characterized in that, described acidic aqueous solution is: the citric acid solution that quality-percent by volume is 0.5-1%.
4. Chinese medicine composition according to claim 1, is characterized in that step 2) extracting method of described Herba Abri is:
Herba Abri adds acid extraction with aqueous solution 2-3 time, adds the acidic aqueous solution that medical material gross weight 8-20 doubly measures at every turn, and each extraction time is 1-4 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.
5. Chinese medicine composition according to claim 1, is characterized in that, Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medicinal material extract methods are described in step 3):
Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add ethanol extraction 2-3 time that percent by volume is 60-80%, add two taste medical material gross weight 6-12 at every turn and doubly measure ethanol, and each extraction time is 1-5 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
6. Chinese medicine composition according to claim 1, is characterized in that, Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medicinal material extract methods are described in step 3):
Stigma Maydis, Herba Plantaginis, Herba lygodii three taste medical materials add ethanol extraction 2-3 time that percent by volume is 70-80%, add two taste medical material gross weight 8-10 at every turn and doubly measure ethanol, and each extraction time is 2-4 hour, merge extractive liquid,, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
7. Chinese medicine composition according to claim 1, is characterized in that, water extraction method is described in step 4):
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water that seven taste Chinese medicine gross weight 6-12 doubly measure at every turn, decoct 1-3 hour, collecting decoction at every turn, filter, filtrate is condensed into fluid extract.
8. Chinese medicine composition according to claim 1, is characterized in that, water extraction method is described in step 4):
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water that seven taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct 2-3 hour, collecting decoction at every turn, filter, filtrate is condensed into fluid extract.
9. Chinese medicine composition according to claim 1, is characterized in that, water extraction method is described in step 4):
In step 3), the alcohol extraction medicinal residues of Stigma Maydis, Herba Plantaginis, Herba lygodii merge Herba Desmodii Styracifolii, Folium Pyrrosiae, Rhizoma Imperatae, Poria extracting in water 2 times, add the water that seven taste Chinese medicine gross weight 8-10 doubly measure at every turn, decoct collecting decoction at every turn 3 hours, filter, filtrate is condensed into fluid extract.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106075128A (en) * | 2016-07-12 | 2016-11-09 | 南方医科大学 | A kind of Chinese medicine composition containing Herba Centellae and application thereof |
| CN112618593A (en) * | 2020-12-01 | 2021-04-09 | 广西中恒创新医药研究有限公司 | A processing method of beta-cyclodextrin inclusion atractylone in Atractylodis rhizoma medicinal material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
-
2013
- 2013-12-30 CN CN201310744143.9A patent/CN103751583A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈锐: "结石通片临床应用解析", 《中国社区医师》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106075128A (en) * | 2016-07-12 | 2016-11-09 | 南方医科大学 | A kind of Chinese medicine composition containing Herba Centellae and application thereof |
| CN106075128B (en) * | 2016-07-12 | 2019-10-01 | 南方医科大学 | A kind of Chinese medicine composition containing centella and its application |
| CN112618593A (en) * | 2020-12-01 | 2021-04-09 | 广西中恒创新医药研究有限公司 | A processing method of beta-cyclodextrin inclusion atractylone in Atractylodis rhizoma medicinal material |
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