CN103705772B - Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof - Google Patents
Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof Download PDFInfo
- Publication number
- CN103705772B CN103705772B CN201310744579.8A CN201310744579A CN103705772B CN 103705772 B CN103705772 B CN 103705772B CN 201310744579 A CN201310744579 A CN 201310744579A CN 103705772 B CN103705772 B CN 103705772B
- Authority
- CN
- China
- Prior art keywords
- herba
- chinese medicine
- medicine composition
- alcoholic solution
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof, the method making this Chinese medicine composition is mainly: get Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperatae totally 8 taste medical materials, Herba Abri citric acid-alcoholic solution extracts, Herba Plantaginis sodium bicarbonate-alcoholic solution extracts, Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria water extraction, finally merge three kinds of extracting method gained fluid extracts.By animal effect experiment and clinical trial, result proves: Chinese medicine composition provided by the invention is when treating urinary system calculus, and antiinflammatory liver protective effect is better than prior art.
Description
Technical field
The present invention relates to the Chinese medicine composition for the treatment of urinary system calculus, be specifically related to Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof.
Background technology
Urinary system calculus belongs to " sand Stranguria ", " stranguria caused by urinary stone " and " stranguria with blood " scope in traditional Chinese medical science gonorrhea, mainly comprises 4 kinds of pattern of syndrome:
(1) qi stagnation and blood stasis type: disease sees waist dull pain, dull pain, the normal or stringy and tense pulse of arteries and veins, tongue is normal; Or urine is interrupted suddenly time excessive, sharp ache, upper even waist abdomen, sandstone is discharged rear pain and is namely delayed; Or waist, flank portion tugging pain as strand, pain extending to the lower abdomen, shows effect again and again, pale complexion, cold sweat, vomiting and nausea during pain, companion's hematuria or urine yellow skin red, dimly red tongue or have petechia, ecchymosis, stringy and tense pulse or slow puckery.
(2) damp heat downward flowing type: card is shown in fever with aversion to cold, lumbago, few abdomen is anxious full, and frequent micturition is short red, and when drowning, puckery pain unbearably, drenches drop not well.Yellow and greasy fur, stringy and rolling pulse or sliding number.
(3) kidney yin deficiency type: calculus is stopped for a long time, pathogenic heat are strongly fragrant for a long time.Kidney-Yin loss, dizzy tinnitus, pain in the low back and legs, dribbling urination or not well, insomnia and dreamful sleep, time have low grade fever, shortness of breath and palpitation, dysphoria with feverish sensation in the chest palms and soles, night sweat, eye is dry or puckery, abdominal distention constipation, poor appetite, thready and rapid pulse, red tongue or few tongue.
(4) kidney yang deficiency syndrome: calculus is stayed for a long time, kidney yang deficiency, the acid of waist lower limb is heavy, and lassitude, whole body is timid cold, and extremities being not warm or the lower part of the body often have creeping chill, frequent micturition or dysuria, frequent urination at night, and complexion is white, and deep pulse is thin and delicate, the white pale tongue of tongue.
" the Sanitation Ministry medicine standard " the 13rd disclosed JIESHITONG PIAN discloses following information:
1) form: Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperatae.
2) method for making: above eight tastes, gets Poria 96g and is ground into fine powder, and seven tastes such as residue Poria and all the other Stigma Maydis etc. decoct with water secondary, each 3 hours, collecting decoction, filtered, filtrate is condensed into the clear paste that relative density is 1.19-1.20 (90 DEG C), adds Poria fine powder, mixing, dry, pulverize into fine powder, sieve, add appropriate amount of auxiliary materials, granule processed, mixing, be pressed into 1000, sugar coating, obtain final product.
3) function with cure mainly: clearing away heat-damp and promoting diuresis, Tonglin Paishi, pain easing and hemostasis.For urinary system infection, cystitis, oedema due to nephritis, lithangiuria, hematuria, drenches drop muddy, urethra causalgia etc.
But in described ministry standard, there is no the proportional quantity of clearly each composition.
The extracting method logical about calculus has following report:
Chinese patent application 01100318.9 specify that the preparation method that calculus is led to is: all pulverizing medicinal materials are nanoscale, microwave extracting, concentrating under reduced pressure, dry.
Chinese patent application 200510032580.3 discloses calculus and leads to the preparations such as ball, drop pill, micropill, dispersible tablet, pellet capsule.Preparation method disclosed in this patent adopts part Poria to pulverize, residue Poria and all the other 7 taste medical material water extractions, or whole medical material water extraction.
Chinese patent application 200510047400.9 discloses a kind of Chinese medicine sugar free granule being used for the treatment of lithangiuria and preparation method thereof.This patent raw material is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, and residue Poria and all the other 7 taste medical material water extractions, adopt low temperature drying method.
Chinese patent application 200910224023.x discloses the treatment pharmaceutical composition of diseases of urinary system and the detection method of component thereof.The raw material that this patent uses is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.Disclose the detection method of Herba Abri in preparation, chlorogenic acid.
Chinese patent application 200910249899.x discloses the preparation method of pharmaceutical composition and the detection method of component thereof for the treatment of diseases of urinary system.The raw material that this patent uses is JIESHITONG PIAN raw material, and disclosed preparation method is that part Poria is pulverized, residue Poria and all the other 7 taste medical material water extractions.Disclose the detection method of Folium Pyrrosiae in preparation.
Because the character of active component is different in eight taste medical materials, as in above-mentioned patent, preparation method all adopts water extraction, some active component can be caused to propose, or extraction ratio is low, affects the performance of drug effect.
Therefore, need to provide a kind of active component extraction ratio that can improve medicine, to improve the preparation method that medicine antiinflammatory protects the liver curative effect.
Summary of the invention
The Chinese medicine composition that the antiinflammatory that the object of this invention is to provide a kind of determined curative effect protects the liver.
The Chinese medicine composition that a kind of antiinflammatory provided by the invention protects the liver, the preparation method of its active component comprises the following steps:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456 parts, Stigma Maydis 302 parts, Folium Pyrrosiae 228 parts, Herba Abri 228 parts, 228 parts, Poria, Herba Plantaginis 165 parts, Herba lygodii 165 parts, Rhizoma Imperatae 228 parts;
2) Herba Abri uses citric acid-alcoholic solution to extract for extracting solvent, and extract makes fluid extract;
3) Herba Plantaginis uses sodium bicarbonate-alcoholic solution to extract for extracting solvent, and extract makes fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water, extract makes fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, to obtain final product.
Described prepare in the Chinese medicine composition step that antiinflammatory protects the liver above:
Step 2) described in citric acid-alcoholic solution matched proportion density be preferably: the 10-40% alcoholic solution of 0.1-5% citric acid;
Step 2) described in citric acid-alcoholic solution matched proportion density more preferably: 30% alcoholic solution of 0.5-1% citric acid.
Step 2) described extracting method be preferably:
Herba Abri adding citric acid-alcoholic solution extracts 2-3 time, and add the citric acid-alcoholic solution of medical material gross weight 8-20 times amount, each extraction time is 1-4 hour, merge extractive liquid, at every turn, and filter, filtrate is condensed into fluid extract.
Citric acid of the present invention-alcoholic solution preparation method is as follows:
Citric acid weight required when citric acid content is 0.1-5% is calculated by required extraction solvent entire volume, and the 95% ethanol contend amount be mixed with needed for 10-40% alcoholic solution, 95% ethanol is first added during preparation, add citric acid again, finally add water to required solvent cumulative volume, stir, to obtain final product.
Sodium bicarbonate described in step 3)-alcoholic solution matched proportion density is preferably: the 10-40% alcoholic solution of 0.1-5% sodium bicarbonate.
Sodium bicarbonate described in step 3)-alcoholic solution matched proportion density is more preferably: sodium bicarbonate-alcoholic solution is: 20% alcoholic solution of 0.5-1% sodium bicarbonate.
Sodium bicarbonate of the present invention-alcoholic solution preparation method is as follows:
Sodium bicarbonate weight required when sodium bicarbonate content is 0.1-5% is calculated by required extraction solvent entire volume, and the 95% ethanol contend amount be mixed with needed for 10-40% alcoholic solution, 95% ethanol is first added during preparation, add sodium bicarbonate again, finally add water to required solvent cumulative volume, stir, to obtain final product.
Extracting method described in step 3) is preferably:
Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 2-3 time, and add the sodium bicarbonate-alcoholic solution of medical material gross weight 10-20 times amount, each extraction time is 1-4 hour, merge extractive liquid, at every turn, and filter, filtrate is condensed into fluid extract.
Extracting method described in step 4) is preferably:
Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water of medical material gross weight 6-12 times amount at every turn, decocts 1-3 hour, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract.
Extracting method described in step 4) is more preferably:
Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water of medical material gross weight 8-10 times amount at every turn, decocts 2-3 hour, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract.
Described in step 4), extracting method is further preferably:
Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 8-10 times amount at every turn, decocts 2-3 hour, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract.
Present invention also offers the preparation containing above-mentioned Chinese medicine composition, said preparation is made up separately of Chinese medicine composition or is made up of Chinese medicine composition and pharmaceutically acceptable carrier.
Described preparation is solid preparation or liquid preparation, and solid preparation is tablet, capsule or granule; Liquid preparation is mixture or oral liquid.
Described pharmaceutically acceptable carrier is in lactose, starch, dextrin, mannitol, sucrose, hydroxypropyl methylcellulose, cross-linking sodium carboxymethyl cellulose, low-substituted hydroxypropyl cellulose, microcrystalline Cellulose, micropowder silica gel, pregelatinized Starch, sorbitol, magnesium stearate, Pulvis Talci, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sodium benzoate, potassium sorbate, sucrose or aspartame, monosodium glutamate, Mel, orange flavor one or more.
The preparation method of Chinese medicinal composition preparation of the present invention, the method can adopt conventional formulation technique to be prepared as various common dosage forms.
Such as:
Method 1: Chinese medicinal composition capsules agent of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add capsule and commonly use adjuvant, production method is prepared into the capsule of Chinese medicine composition of the present invention routinely.
Method 2: Chinese medicine composition tablet of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add tablet and commonly use adjuvant, production method is prepared into the tablet of Chinese medicine composition of the present invention routinely.
Method 3: Chinese medicinal composition granules of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, be dried to dried cream powder, add granule and commonly use adjuvant, production method is prepared into the granule of Chinese medicine composition of the present invention routinely.
Method 4: Chinese medicine composition mixture of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add mixture and commonly use adjuvant, production method is prepared into the mixture of Chinese medicine composition of the present invention routinely.
Method 5: Chinese medicine composition oral liquid of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add oral liquid and commonly use adjuvant, production method is prepared into the oral liquid of Chinese medicine composition of the present invention routinely.
Method 6: Chinese medicine composition syrup of the present invention
Get Chinese medicine composition preparation process 5) middle gained Chinese medicine composition, add syrup and commonly use adjuvant, production method is prepared into the syrup of Chinese medicine composition of the present invention routinely.
Present invention also offers the application of above-mentioned Chinese medicine composition or preparation raising antiinflammatory liver protective effect when preparing treatment urinary system calculus disease.
Urinary system calculus belongs to stranguria caused by urinary stone, sand Stranguria in traditional Chinese medical science gonorrhea, due to damp invasion of lower energizer, changes the scorching the moon that burns, decocts urine, become sandstone, alluvial water channel, and be stranguria caused by urinary stone.Amass in lower then bladder qi dysfunction, it is unfavorable to urinate, very then for go out can not, poverty-strickenly to feel bad, pain extending to the lower abdomen; Be stranded in, then affect the duty of kidney department urine, pent-up must not be let out down, and QI and blood is unsmooth, stagnation of QI and blood may bring about pain, is involved bladder, pudendum by kidney; It is then hematuria that sandstone hinders network.Drench and do not heal for a long time, damp and hot harmful spleen, so that deficiency-weakness of spleen-QI, sinking of QI of middle-JIAO, temper is not prosperous, dysfunction of the spleen in transportation and transformation, causes that blood biochemical is not enough, liver-blood deficiency.Therefore, treatment stranguria caused by urinary stone while washing away sandstone, diuresis promoting, should be treated the infection symptoms that urinary system occurs, prevents inflammation from expanding further, have influence on other normal organs in time.Meanwhile, the hepatic injury that maintenance causes because suffering from gonorrhea is for a long time answered.The state of an illness is made to be able to thoroughly fully recover as early as possible
Chinese medicine composition effective ingredient disclosed in this invention is prepared from by raw materials such as Herba Desmodii Styracifolii, Stigma Maydis, Folium Pyrrosiae, Herba Abri, Poria, Herba Plantaginis, Herba lygodii, Rhizoma Imperataes.Inventor finds in preparation research process for many years, identical drug dose, different extraction processes prepare the active component of gained, also larger difference and emphasis can be there is in its pharmacological action, for this reason, applicant has carried out a series of technical study to Chinese medicine composition of the present invention in conjunction with pharmacological evaluation, sum up process for extracting of the present invention, and bring drug effect potentiation through the improvement that experimental verification is such: Herba Abri citric acid-alcoholic solution extracts, Herba Plantaginis sodium bicarbonate-alcoholic solution extracts, Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria water extraction, when finally the fluid extract of merging three kinds of extracting method gained is used for the treatment of urinary system calculus, its antiinflammatory hepatoprotective effect is far superior to the existing extractive technique of identical prescription, clinical efficacy shows,
Compared with prior art, antiinflammatory protects the liver curative effect, and there were significant differences for extract of the present invention, has significance progress.
Inventor detects Chinese medicine composition of the present invention, finds:
1, be that the alkaloids component content of representative is very high with abrine, compared with existing extraction process products obtained therefrom, both have notable difference, may be because in Herba Abri, specific examples of such components is soluble in acid ethanol solution, so extract more complete in citric acid-alcoholic solution.
2, be that the iridoid glycosides component content of representative is very high with aucubin, compared with existing extraction process products obtained therefrom, both have notable difference, may be because specific examples of such components is soluble in alkaline ethanol solution, so extract more complete in sodium bicarbonate-alcoholic solution.
Above-mentioned testing result indicates the extraction of some effective ingredient in each component from the side and is better than prior art.Because every taste Chinese crude drug is all containing many active component, at least not yet there is any a herb purely and simply to be analyzed and know all the components, when different Chinese medicine combines and extracts with different extracting mode, due to influencing each other and acting between solvent, temperature and heterogeneity, very complicated chemical reaction can be produced.Therefore, above-mentioned effective ingredient is a part for Chinese medicine extract active component, and the drug action impact for overall medicine is still not clear.We can only contrast the quality of different extracting mode in certain therapeutic use by effect experiment at present, there is no the change indicator of method from part effective ingredient to judge the curative effect change of compound Chinese patent medicine.
We contrast the curative effect of different extracting mode antiinflammatory by " impact of the rat paw edema caused by Carrageenan " zoopery; The effect of different extracting mode in protecting the liver is contrasted by " impact on acute liver injury of rats ".
Inventor has carried out a large amount of systematic research experiment to the extracting method of each medical material that calculus is led to and the excellent effect of medicine, by medical material is split as many groups, extract with diverse ways, finally obtain extractive technique scheme of the present invention, make the medical material of same recipe, given play to that therapeutic purposes are clearer and more definite, the effect of more remarkable treatment effect.
The Chinese medicine composition that a kind of antiinflammatory provided by the invention protects the liver has the following advantages:
1, Chinese medicine composition hepatoprotective effect of the present invention is obvious, and after administration, rat hepatic cords arrangement compared with model group rats aligns mutually, necrosis region obviously reduces inflammatory cell infiltration and significantly reduce, and of the present invention group comparatively comparative example group effect is more remarkable.In addition, hepatic tissue pathology section microscopy result also demonstrate that Chinese medicine composition provided by the invention can alleviate stem cell degeneration, necrosis, hemorrhage, promotes liver cell regeneration, plays good protective effect to liver.
2, Chinese medicine composition antiinflammatory action of the present invention is obvious, and the rat paw edema caused by Carrageenan alleviates significantly (P < 0.01, P < 0.05); And the present invention compares with comparative example, there is pole significant difference (P < 0.01) or significant difference (P < 0.05).Test of pesticide effectiveness result shows, Chinese medicine composition antiinflammatory action provided by the invention is better than prior art
3, clinical test results display: the present invention is good to antiinflammatory, liver protective effect, and curative effect of the present invention is better than matched group, antiphlogistic effects has significant difference (P<0.05) compared with matched group, and hepatoprotective effect has pole significant difference (P<0.01).The improvement effect of medicine of the present invention to Urinary system inflammation and liver function is better than JIESHITONG PIAN.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
If no special instructions, the concentration of alcohol in the present invention is percent by volume.
Embodiment 1:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 2 times, and add 10% alcoholic solution of 0.1% citric acid of medical material gross weight 20 times, 15 times amount respectively, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid, at every turn, and filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 2 times, each 10% alcoholic solution of 0.1% sodium bicarbonate adding medical material gross weight 20 times, 15 times amount respectively, each extraction time is respectively 4 hours, 2 hours, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 12 times, 10 times amount at every turn respectively, decocts 3 hours, 2 hours respectively, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 2:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 3 times, each add medical material gross weight 20 times, 15 times, 40% alcoholic solution of 5% citric acid of 8 times amount respectively, each extraction time is respectively 4 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 3 times, each add medical material gross weight 20 times, 15 times, 40% alcoholic solution of 5% sodium bicarbonate of 10 times amount respectively, each extraction time is respectively 4 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 3 times, each add medical material gross weight 12 times, 10 times, the water of 6 times amount respectively, decoct 3 hours, 2 hours, 1 hour respectively, collecting decoction at every turn, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 3:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 3 times, each add medical material gross weight 20 times, 15 times, 20% alcoholic solution of 0.5% citric acid of 10 times amount respectively, each extraction time is respectively 3 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 3 times, each add medical material gross weight 20 times, 15 times, 30% alcoholic solution of 0.5% sodium bicarbonate of 10 times amount respectively, each extraction time is respectively 3 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 3 times, each add medical material gross weight 10 times, 8 times, the water of 8 times amount respectively, decoct 3 hours, 2 hours, 2 hours respectively, collecting decoction at every turn, filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 4:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 2 times, and add 20% alcoholic solution of 1% citric acid of medical material gross weight 20 times, 10 times amount respectively, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid, at every turn, and filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 2 times, and add 30% alcoholic solution of 1% sodium bicarbonate of medical material gross weight 20 times, 10 times amount respectively, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid, and filter, filtrate is condensed into fluid extract at every turn;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 10 times, 10 times amount at every turn respectively, decocts 3 hours, 2 hours respectively, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 5:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 3 times, each add medical material gross weight 20 times, 15 times, 40% alcoholic solution of 0.1% citric acid of 15 times amount respectively, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 3 times, each add medical material gross weight 20 times, 15 times, 40% alcoholic solution of 0.1% sodium bicarbonate of 15 times amount respectively, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 10 times, 8 times amount at every turn respectively, decocts 3 hours, 2 hours respectively, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 6:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 3 times, each add medical material gross weight 20 times, 15 times, 10% alcoholic solution of 5% citric acid of 10 times amount respectively, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 3 times, each add medical material gross weight 20 times, 15 times, 10% alcoholic solution of 5% sodium bicarbonate of 15 times amount respectively, each extraction time is respectively 2 hours, 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 10 times, 8 times amount at every turn respectively, decocts 3 hours, 2 hours respectively, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 7:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
2) Herba Abri adding citric acid-alcoholic solution extracts 2 times, and add 20% alcoholic solution of 0.1% citric acid of medical material gross weight 10 times, 8 times amount respectively, each extraction time is respectively 2 hours, 2 hours, merge extractive liquid, at every turn, and filter, filtrate is condensed into fluid extract;
3) Herba Plantaginis adds sodium bicarbonate-alcoholic solution and extracts 2 times, each 30% alcoholic solution of 0.1% sodium bicarbonate adding medical material gross weight 15 times, 8 times amount respectively, each extraction time is respectively 2 hours, 1 hour, merge extractive liquid, filter, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 10 times, 10 times amount at every turn respectively, decocts 3 hours, 2 hours respectively, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, obtain Chinese medicine composition.
Embodiment 8: capsule
1, form: the fluid extract of embodiment 1 gained, is dried to dried cream powder, obtains 316g, adds pregelatinized Starch 69g, magnesium stearate 6.4g, micropowder silica gel 6.4g.
2, preparation method: the method with reference to conventional Chinese medicine preparation makes capsule.
Embodiment 9: tablet
1, form: the fluid extract of embodiment 2 gained, is dried to dried cream powder, obtains 318g, adds microcrystalline Cellulose 103g, pregelatinized Starch 8.8g, micropowder silica gel 5.7g.
2, preparation method: the method with reference to conventional Chinese medicine preparation makes tablet.
Embodiment 10: granule
1, form: the fluid extract of embodiment 3 gained, is dried to dried cream powder, obtains 315g, adds sucrose 1310g, pregelatinized Starch 156g.
2, preparation method: the method with reference to conventional Chinese medicine preparation makes granule.
Embodiment 11: oral liquid
1, form: embodiment 4 gained fluid extract 4.6L (relative density 1.050 ~ 1.065/30 DEG C), sucrose 550g, monosodium glutamate 8.7g, sodium benzoate 5.4g.
2, preparation method: fluid extract is heated to more than 80 DEG C, then adds sucrose and antiseptic, embedding, sterilizing, makes oral liquid.
Embodiment 12: syrup
1, form: embodiment 5 gained fluid extract 9.0L (relative density 1.015 ~ 1.035/30 DEG C), sucrose 8.2kg, Mel 1.0kg, sodium benzoate 22g.
2, preparation method: the method with reference to conventional Chinese medicine preparation makes syrup.
Comparative example 1:
With reference to " the Sanitation Ministry medicine standard " the 13rd disclosed JIESHITONG PIAN methods of extraction and preparation, specific as follows:
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, get Poria 96g and are ground into fine powder, and seven tastes such as residue Poria and all the other Stigma Maydis etc. decoct with water secondary, each 3 hours, collecting decoction, filter, filtrate is condensed into the clear paste that relative density is 1.19 (90 DEG C), add Poria fine powder, mixing, dry, pulverize into fine powder, sieve, to obtain final product.
Comparative example 2: full water extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, extracting in water 3 times, adds the water of medical material gross weight 10 times amount at every turn, each decoction 3 hours, collecting decoction, filters, and when filtrate is concentrated into 70 DEG C, relative density is the clear paste of 1.10, add concentration be 95% ethanol make alcohol content reach 60%, leave standstill 12 hours, filter, when filtrate is concentrated into 70 DEG C, relative density is the clear paste of 1.15, be dried to dried cream powder, obtain final product.
Comparative example 3: full alcohol extraction
Take Herba Desmodii Styracifolii 456g, Stigma Maydis 302g, Folium Pyrrosiae 228g, Herba Abri 228g, Poria 228g, Herba Plantaginis 165g, Herba lygodii 165g, Rhizoma Imperatae 228g;
Above eight tastes, with ethanol extraction 3 times, add medical material gross weight 10 times amount 80% ethanol at every turn, extract 2 hours at every turn, and merge extractive liquid, filters, and when filtrate is concentrated into 60 DEG C, relative density is the clear paste of 1.15, is dried to dried cream powder, obtains final product.
Experimental example 1: Herba Abri DNA extration
With different extracting method, Herba Abri is extracted, by the antiphlogistic effects of the more different extract of animal effect experiment.
1 extracts solvent: 30% ethanol of 0.1% citric acid, 30% alcoholic solution, water.
2 extracting method: take Herba Abri by recipe quantity, add above-mentioned Different solution and extract 3 times respectively, each add medical material gross weight 12 times, 10 times, the solvent of 10 times amount respectively, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
3 antiphlogistic effects test methods: the impact of the rat paw edema caused by Carrageenan
4 analysis results are in table 1
The impact of the rat paw edema caused by table 1 Different Extraction Method on Carrageenan
Note: compare with blank group, * P<0.05, * * P<0.01; Compare with 30% alcoholic solution experimental group of 0.1% citric acid, #P<0.05.
Table 1 result shows: with the extract of 30% alcoholic solution of 0.1% citric acid for solvent extraction gained, caused by Carrageenan, rat paw edema alleviates the highest, antiphlogistic effects is best, has significant difference (P<0.05) compared with 30% ethanol experimental group, water experimental group.
Experimental example 2: Herba Plantaginis DNA extration
With different extracting method, Herba Plantaginis is extracted, by the analgesic activity of the more different extract of animal effect experiment.
1 extracts solvent: 20% ethanol of 0.5% sodium bicarbonate, 20% alcoholic solution, water.
2 extracting method: take Herba Plantaginis by recipe quantity, add above-mentioned Different solution and extract 3 times respectively, each add medical material gross weight 12 times, 10 times, the solvent of 10 times amount respectively, each extraction time is respectively 5 hours, 4 hours, 3 hours, merge extractive liquid, filter, reclaim medicinal residues, filtrate is condensed into fluid extract.
3 protect the liver test method: on the impact of acute liver injury of rats.
4 analysis results are in table 2
Table 2 is group rat ALT, AST expression activitiy respectively
Note: compare with Normal group, * * P<0.01; Compare with model control group, #P<0.05, ##P<0.01; Compare with 20% alcoholic solution experimental group of 0.5% sodium bicarbonate; Compare with the sodium bicarbonate solution experimental group of 0.1%, ◆ ◆ P<0.01.
Table 2 result shows: with the extract of 20% ethanol of 0.5% sodium bicarbonate for solvent extraction gained, hepatic cords arrangement aligns mutually compared with model group rats, necrosis region obviously reduces inflammatory cell infiltration and significantly reduce, and of the present invention group more remarkable compared with 20% ethanol experimental group, water experimental group effect.In addition, hepatic tissue pathology section microscopy result also demonstrate that Chinese medicine composition provided by the invention can alleviate stem cell degeneration, necrosis, hemorrhage, promotes liver cell regeneration, plays good protective effect to liver.
Experimental example 3: the comparison of Chinese medicine composition extracting method
1 sample grouping
Embodiment 1-7, comparative example 1-3 make dried cream powder respectively, grouping.
2 contrast projects
Alkaloids content: the alkaloids effective ingredient with high effective liquid chromatography for measuring with abrine being contrast;
Iridoid glycosides content: the iridoid glycoside effective constituents with high effective liquid chromatography for measuring with aucubin being contrast;
3 experimental results, in table 3
Table 3 Different Extraction Method is to the extraction effect of medical material
| Group | Abrine (mgg-1) | Aucubin (mgg-1) |
| Embodiment 1 | 0.811±1.64 | 0.369±1.01 |
| Embodiment 2 | 0.809±1.58 | 0.364±1.13 |
| Embodiment 3 | 0.805±1.53 | 0.378±1.07 |
| Embodiment 4 | 0.811±1.60 | 0.359±1.06 |
| Embodiment 5 | 0.816±1.57 | 0.371±1.03 |
| Embodiment 6 | 0.814±1.56 | 0.358±1.12 |
| Embodiment 7 | 0.796±1.57 | 0.376±1.07 |
| Comparative example 1 | 0.461±1.62 | 0.235±1.06 |
| Comparative example 2 | 0.508±1.59 | 0.247±1.12 |
| Comparative example 3 | 0.473±1.61 | 0.239±1.08 |
Table 3 result shows: embodiment of the present invention 1-7, with abrine be representative alkaloids composition, be that the iridoid glycosides active constituent content of representative is better than comparative example 1-3 with aucubin.Experimental result points out the technology of the present invention extraction alkaloid component and total flavones compared with prior art, extracts more complete.
Antiinflammatory protects the liver effect experiment
The impact of the rat paw edema one, caused by Carrageenan
1, experiment material
(1) animal
SD rat, male, body weight 200 ± 20g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
(2) medicine and instrument
Embodiment of the present invention 1-7, comparative example 1-3,0.9% normal saline (Pharmaceutical Group Co., Ltd of Nanning Baihui), dexamethasone (Zhejiang Xian Ju pharmaceutical Co. Ltd), carrageenin (the new YounHa Chemical Co., Ltd. in Hubei), YLS-7C toes capacity measurer (Jinan Yi Yan Science and Technology Ltd.).
2, experiment grouping
Select healthy SD rat 120, female, be divided into 12 groups at random, often organize 10:
Blank group: gavage equal-volume normal saline;
Experimental group: give embodiment 1-7, comparative example 1-3 medicine;
Positive controls: gavage equal-volume dexamethasone medicinal liquid.
3, experimental technique
Each treated animal gastric infusion every day 1 time, positive controls gives dexamethasone 0.02g/kg/d, and embodiment 1-7 group and comparative example 1-3 group are by crude drug amount 3.1g/kg/d, and blank group gives equal-volume normal saline, continuous gastric infusion 7d.After last administration, 1h causes inflammation at Rat Right ankle joint injection 1% carrageenin suspension 0.1mL, and respectively before causing inflammation and cause scorching after 6h by measure method measure rat toes to the volume of ankle joint, calculate paw swelling as follows:
Swelling (%)=(toes volume after causing inflammation-cause scorching front toes volume)/cause scorching front toes volume × 100%.
4, experimental result
The impact of rat paw edema caused by each test group on Carrageenan of table 4
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with comparative example 1 group, △ P < 0.05, △ △ P < 0.01; Compare with comparative example 2 groups, ▲ P < 0.05, ▲ ▲ p < 0.01; Compare with comparative example 3 groups, ◆ P < 0.05, ◆ ◆ P < 0.01.
Table 4 result shows, and compared with blank group, embodiment 1-7 group, comparative example 1-3 group all reduce rat paw edema degree caused by carrageenin, and Be very effective (P < 0.01, P < 0.05); Compared with comparative example 1-3 group, embodiment 1-7 group rat paw edema degree is starkly lower than comparative example 1-3 group (P < 0.01, P < 0.05).
Result shows: Chinese medicine composition provided by the invention has antiinflammatory action, and effect is better than prior art.
Two, to the effect of acute hepatic injury model mice
1, experiment material
(1) animal
Healthy SPF level Kunming mouse, weight 18 ~ 22g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
(2) medicine and reagent
Embodiment of the present invention 1-7, comparative example 1-3,0.9% normal saline (Pharmaceutical Group Co., Ltd of Nanning Baihui), carbon tetrachloride (AR, Aladdin), formaldehyde (AR, Aladdin), ALT(alanine aminotransferase), AST(aspartic transaminase) measure test kit (Bioengineering Research Institute is built up in Nanjing); Hematoxylin-eosin solution (Foochow steps new).
2, experiment grouping
Get mice 120, male and female half and half, be divided into 12 groups at random: Normal group, model control group, embodiment 1-7 group, comparative example 1-3 group, often organize 10.
3, experimental technique
Each group of embodiment 1-7 and comparative example 1-3 respectively organizes dosage all by crude drug amount 4.5g/kg/d, every day gavage 1 time; Normal group and model group give isometric(al) 0.9% normal saline solution, continuous 14d.1h after last administration, except normal group, each group mice equal lumbar injection 1%CC14 peanut oil solution, 10mL/kg, the vegetable oil of blank group injection equivalent.After modeling 24h, water is can't help in fasting, and 2d gets blood through eye socket, the centrifugal 10min of 3500rmp, separation of serum,
Detect Serum ALT, AST is active.Get hepatomegaly leaf, 10% formalin is fixed, specimens paraffin embedding slices, conventional hematoxylin-eosin stains, observes hepatic tissue pathology and changes.
4, experimental result
The activity of table 5 each test group mice serum ALT and AST
Note: compare with Normal group, * P < 0.05, * * P < 0.01; Compare with model control group, ☆ P < 0.05, ☆ ☆ P < 0.01; Compare with comparative example 1 group, △ P < 0.05, △ △ P < 0.01; Compare with comparative example 2 groups, ▲ P < 0.05, ▲ ▲ p < 0.01; Compare with comparative example 3 groups, ◆ P < 0.05, ◆ ◆ P < 0.01.
Conclusion:
1) CCl
4the activity of hepatic injury mice serum ALT and the AST of induction
Table 5 result shows, and compared with Normal group, the content of model group Serum ALT and AST obviously raises (P < 0.01), and show that now mouse liver cell damages, model is successfully prepared.Compared with model group, embodiment 1-7 group and comparative example 1-3 group serum alt, AST content all significantly reduce (P < 0.01, P < 0.05).Illustrate that they can reduce CCl
4to the dissolution of liver plasma membrane, to liver plasma membrane, there is certain protective effect.And embodiment 1-7 group ALT, AST content are compared with comparative example 1-3 group, there is significant difference (P < 0.01, P < 0.05).
2) hepatic tissue pathology inspection
After sacrifice, cut open the belly and get liver, observe liver color, find that naive mice liver color is bronzing, moistening glossy, high resilience, more blank group of model group Mouse Liver color is shallow, microscopy murine liver tissue is cut into slices, and can be observed acute liver damage blank group lobules of liver structure normal, liver rope marshalling.Sinus hepaticus is obvious, and clear in structure can be distinguished, cytoplasm of liver is many, and nucleus is large, has no hepatocellular degeneration necrosis.And the lobules of liver of model group mice loses normal configuration, liver rope arrangement disorder, there is the necrosis of larger area in hepatic tissue, namely focus point is a lot, hepatocyte is downright bad companion's cell infiltration in various degree. the visible cloudy swelling of part, and hepatocyte volume increases, and presents balloon sample pathological changes, core differs in size, in pyknosis in various degree.And the above-mentioned hepatic injury phenomenon of embodiment 1-7 group and comparative example 1-3 group all has and alleviates in various degree, and embodiment 1-7 group comparatively comparative example 1-3 group effect is more remarkable.In addition, hepatic tissue pathology section microscopy result also demonstrate that Chinese medicine composition provided by the invention can alleviate stem cell degeneration, necrosis, hemorrhage, promotes liver cell regeneration, plays good protective effect to liver.
Result shows, Chinese medicine composition provided by the invention has protective effect to hepar damnification,
And effect is better than prior art.
Clinical trial:
Clinical observation is carried out to the curative effect that present anti-inflammatory protects the liver, analyzes antiinflammatory liver protective effect of the present invention by the liver function situation and anti-inflammatory effects contrasting Patients Before And After of taking medicine.
1 diagnosis basis
1.1 Urinary system inflammation
1.1.1 symptom
Disease sees shiver with cold, fever, urgent micturition, frequent micturition, dysurea, lumbago, unable, hematuria, pyuria etc., chronic phase inflammation or inflammation recurrent exerbation can there are edema, hypertension, albuminuria etc.
1.1.2 routine urianlysis
Specific gravity of urine increases or reduces, urine protein positive, glucose in urine positive, and microscopy finds that erythrocyte is greater than 3/HP, finds leukocyte and epithelial cell, cast etc.
1.1.3 imaging examination
Inflammation part and degree can be determined further by plain abdominal radiograph, urography, MRI etc.
1.2 abnormal liver function
1.2.1 protein metabolism functional check: total serum protein, albumin, globulin, albumin and globulin ratio A/G etc. are higher or lower than clinical normal value.
1.2.2 bilirubin metabolism functional check: detect serum total bilirubin, bilirubin direct and unconjugated bilirubin.
1.2.3 serum enzyme inspection: serum alanine aminotransferase (ALT) normal reference value: colorimetry is 5-25U(karmen unit), performance rate method <40U/L(37 DEG C); Serum aspartate amino transferase (AST) normal reference value 8-12U; Serum alkaline phosphatase (ALP) normal reference value: colorimetry 3-13 King unit, performance rate method <240U/L.
2 case selections
At 20 ~ 70 years old age, be ready acceptance 2 weeks therapists, signature enters clinical research Informed Consent Form person.
3 medicines
Experimental group: embodiment 3
Matched group: calculus is led to, Guangxi Wuzhou Pharmaceutical (group) limited company
4 curative effect index
4.1 urine routine test
Cure: clinical symptoms and sign all disappear; Urine routine test total negative;
Take a turn for the better: clinical symptom disappearance; Urine routine test has a little erythrocyte;
Invalid: symptom is not improved, urine routine test has erythrocyte, urine protein, epithelial cell;
4.2 liver function test
Effective: clinical symptoms is clearly better, hepatosplenomegaly reduces or stablizes constant, and liver function test ALT, TB recover normal;
Effective: clinical symptoms take a turn for the better or stablize constant, hepatosplenomegaly is stablized constant, liver function test ALT, AST or (with) TB comparatively before reduce more than >50%;
Invalid: without changing, not reach above standard or disease progression person.
5 clinical grouping and Therapeutic Method
Enter to organize the routine patient of case 286, man 156 example, female 130 example; Average about 46 years old; All patients are all diagnosed as Urinary system inflammation and Liver function grade exception person through color ultrasound or the inspection of abdomen plain film, and case is divided into two groups at random.
Therapeutic Method:
Experimental group: the embodiment of the present invention 5, is equivalent to crude drug 10g each serving consumption, three times on the one.
Matched group: JIESHITONG PIAN, each 1.5g, three times on the one.
Experimental group and matched group are being taken medicine simultaneously, and strengthen amount of drinking water every day, average daily drink amount is 3000 ~ 5000ml, takes exercises more, and based on jumping exercise or going upstairs motion, both hands are in dorsal part intersection knocking back lumbar part.
6 results
After in experimental example group case, most of patient takes the embodiment of the present invention 3, paroxysmal lumbago companion hematuria phenomenon reduces or disappears, and checks, calculus moves down, reduce or disappear through color ultrasound or abdomen plain film.
Table 6: routine urianlysis result after treatment
Note: compare with matched group, * P < 0.05.
Table 7: liver function test result after treatment
Note: compare with matched group, * P < 0.01.
Interpretation of result: the routine urianlysis result after two groups of patient treatments is analyzed, experimental group and matched group routine urinalysis, liver protective effect are all good, but the curative effect of experimental group is more excellent, antiphlogistic effects has significant difference (P<0.05) compared with matched group, hepatoprotective effect has pole significant difference (P<0.01).The improvement effect of medicine of the present invention to Urinary system inflammation and liver function is better than JIESHITONG PIAN.
Model case:
1. Zeng, male, 60 years old.The right lumbago of readme numb half a year, recent aggravation, has low grade fever, removes examination in hospital with 2008-11-12 day, and B ultrasonic points out 7 pieces, the multiple calculus of right kidney, and upper ureter has high density shadow, and diameter is 0.73cm, liver function test ALT:122, AST:103.Because patient requests takes expectant treatment, give the embodiment of the present invention 12 syrup, 3 times/day, each 50ml through suggestion.And coordinate jump action and day drink boiled water about 4000ml, after taking medicine 3 days, symptom alleviates, and discharge 3 pieces, calculus during the 6th day urine, maximum diameter is about 0.7cm, and within the 11st day, discharge 2 pieces, calculus again, diameter is about 0.4cm.Within 20th day, check routine urinalysis is normal, and liver function recovery is obvious.It is 2 pieces that B ultrasonic detects the right renal calculus number of prompting, and ureter has no calculus bladder and is showed no calculus, and ALT:42 is shown in liver function test, and AST:38(is without Clinicopathological significance).
2. Huang, man, 50 years old.Dribbling urination accompanies lumbago a couple of days not to the utmost, and lumbago is very with left side, frequent micturition, difficulty in micturition.Red tongue with yellow fur, wiry and frequent pulse.Urine examination albumen is a little, erythrocyte +++.B ultrasonic is detected and is seen in left kidney have a Semen Glycines size high light group, rear accompanying sound shadow, with slight hydrops, is diagnosed as renal calculus companion hydrops; Liver function test ALT:202, AST:78.Tcm diagnosis belong to stranguria damp and hot and heavy.Give the embodiment of the present invention 8 capsule, 3 times on the 1st, each 5.After taking medicine 3 days, all diseases subtract greatly, and its pain is abdomen radiation less left indistinctly.Blood urinary stuttering during urine after taking medicine 6 days, drop and under, urethra has swollen sensation of pricking and discharges 1 piece, calculus (about 0.6cm × 0.5cm).Take medicine ultrasound diagnosis urinary system no abnormality seen light group on the 8th, routine blood test no abnormality seen, ALT:53 is shown in liver function routine examination, AST:32, and disease is almost recovered.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (5)
1. the Chinese medicine composition that protects the liver of antiinflammatory, is characterized in that the preparation method of its active component comprises the following steps:
1) medical material of following weight portion is got: Herba Desmodii Styracifolii 456 parts, Stigma Maydis 302 parts, Folium Pyrrosiae 228 parts, Herba Abri 228 parts, 228 parts, Poria, Herba Plantaginis 165 parts, Herba lygodii 165 parts, Rhizoma Imperatae 228 parts;
2) Herba Abri add 0.1-5% citric acid 10-40% alcoholic solution extract 2-3 time, add the citric acid-alcoholic solution of medical material gross weight 8-20 times amount, each extraction time is 1-4 hour, merge extractive liquid, at every turn, filtration, filtrate is condensed into fluid extract;
3) Herba Plantaginis add 0.1-5% sodium bicarbonate 10-40% alcoholic solution extract 2-3 time, add the sodium bicarbonate-alcoholic solution of medical material gross weight 10-20 times amount, each extraction time is 1-4 hour, merge extractive liquid, at every turn, filtration, filtrate is condensed into fluid extract;
4) Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water of medical material gross weight 6-12 times amount at every turn, decocts 1-3 hour, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract;
5) by step 2), 3), 4) mixing of gained three kinds of fluid extracts, to obtain final product.
2. Chinese medicine composition according to claim 1, is characterized in that, described citric acid-ethanol is: 30% alcoholic solution of 0.5-1% citric acid.
3. Chinese medicine composition according to claim 1, is characterized in that, described sodium bicarbonate-alcoholic solution is: 20% alcoholic solution of 0.5-1% sodium bicarbonate.
4. Chinese medicine composition according to claim 1, is characterized in that, step 4) described water extraction method is:
Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2-3 time, add the water of medical material gross weight 8-10 times amount at every turn, decocts 2-3 hour, collecting decoction at every turn, and filter, filtrate is condensed into fluid extract.
5. Chinese medicine composition according to claim 1, is characterized in that, step 4) described water extraction method is:
Herba Desmodii Styracifolii, Herba lygodii, Folium Pyrrosiae, Stigma Maydis, Rhizoma Imperatae, Poria extracting in water 2 times, add the water of medical material gross weight 8-10 times amount at every turn, decocts 3 hours at every turn, and collecting decoction, filter, filtrate is condensed into fluid extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310744579.8A CN103705772B (en) | 2013-12-30 | 2013-12-30 | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310744579.8A CN103705772B (en) | 2013-12-30 | 2013-12-30 | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103705772A CN103705772A (en) | 2014-04-09 |
| CN103705772B true CN103705772B (en) | 2016-04-27 |
Family
ID=50399359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310744579.8A Active CN103705772B (en) | 2013-12-30 | 2013-12-30 | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103705772B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108354077A (en) * | 2018-03-07 | 2018-08-03 | 余姚辉农农业科技有限公司 | A kind of first farrowing sow feed addictive, feed and preparation method thereof |
| CN111972539A (en) * | 2020-08-19 | 2020-11-24 | 中兽医(汤阴)生物科技有限公司 | Additive for improving immunity of livestock and poultry organisms and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
-
2013
- 2013-12-30 CN CN201310744579.8A patent/CN103705772B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947781A (en) * | 2005-10-14 | 2007-04-18 | 王玫 | Sugar-free traditional Chinese medicine granules for treating lithangiuria and its prepn. method |
| CN1824178A (en) * | 2005-12-20 | 2006-08-30 | 王衡新 | Chinese medicinal preparation for treating urinary system disease, its preparation method and quality control method |
| CN102266501A (en) * | 2011-07-25 | 2011-12-07 | 王疆宏 | Jieshitong tea composition and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 结石通片临床应用解析;陈锐;《中国社区医师》;20120805;9 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103705772A (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100531769C (en) | Chinese medicine composition for treating gout and preparing method thereof | |
| CN102600423B (en) | Preparation method of Chinese medicine for treating hepatic fibrosis | |
| CN103127321A (en) | Traditional Chinese medicine composition for treating prostatitis and preparation method thereof | |
| CN108714194A (en) | A kind of natural composition prevented or treat hyperuricemia and gout | |
| CN103735900A (en) | Calculus-suppressing and pain-alleviating Chinese medicinal composition and preparation method thereof | |
| CN103893284B (en) | A kind of Chinese medicine composition treating chronic kidney disease and chronic renal failure and its production and use | |
| CN103705772B (en) | Chinese medicine composition that a kind of antiinflammatory protects the liver and preparation method thereof | |
| CN101978982B (en) | Application of Japanese ginseng or extract thereof to preparation of medicament for resisting gout and repairing hyperuricemic kidney injury | |
| CN101850063A (en) | Medicinal preparation for preventing and treating gout and preparation method | |
| CN103705773A (en) | Traditional Chinese medicine composition for achieving antibacterial and anti-inflammation effects and preparation method thereof | |
| CN104189267A (en) | Traditional Chinese medicine composition for treating kidney stone and preparation method of traditional Chinese medicine composition | |
| CN103751583A (en) | Traditional Chinese medicine composition for being diuretic and protecting liver and preparation method of composition | |
| CN103933154B (en) | A kind of Chinese medicine composition treating goat and preparation method thereof | |
| CN102552767B (en) | Traditional Chinese medicine for treating kidney stone, preparation method and administration mode | |
| CN103893286B (en) | It is a kind of to treat Chinese medicine preparation of chronic renal disease and preparation method thereof | |
| CN106177268A (en) | A kind of Chinese medicine composition treating sexual impotence and preparation method thereof | |
| CN103705775B (en) | One protects the liver analgesia Chinese medicine composition and preparation method thereof | |
| CN103735901A (en) | Diuretic and anti-inflammatory traditional Chinese medicine composition and preparation method thereof | |
| CN104857070A (en) | Pharmaceutical composition used for resisting intestinal tract inflammatory injuries, and preparation method and applications thereof | |
| CN102078367B (en) | Use of aidi preparation in preparation of medicaments for treating multiple organ dysfunction syndrome in elderly | |
| CN102940656B (en) | Medicine composition for treating benign prostatic hyperplasia | |
| CN102940674B (en) | Medicine composition for treating benign prostatic hyperplasia | |
| CN102940697B (en) | Medicine for treating allergic rhinitis and bronchial asthma | |
| CN102772458B (en) | A kind of medicine for the treatment of cervical erosion | |
| CN108815357B (en) | A Chinese medicinal composition with antipyretic effect, and its preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |