CN103271983B - A kind of endometritis and colpitic perfusion liquid, its preparation method and application for the treatment of pig - Google Patents
A kind of endometritis and colpitic perfusion liquid, its preparation method and application for the treatment of pig Download PDFInfo
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Abstract
The invention provides a kind of endometritis and colpitic perfusion liquid for the treatment of pig, its active ingredient is sophora alapecuroides. The present invention also provides its preparation method and application. That perfusion liquid of the present invention has is antibacterial, anti-inflammatory, low toxicity, noresidue, be difficult for producing the advantages such as drug resistance, excitant is little, cost is lower, can effectively treat the disease such as endometritis, vaginitis of the pig causing because of bacterium, fungi, trichomonad, the particularly endometritis of targeted therapy pig, vaginitis. The present invention has the extract of single medicinal material, is conducive to stability and the quality controllability of product; Targeting preparation discharges in the endometrium of pig and vagina, reduces gastral absorption, directly discharge and can be scattered in the endometrium and vagina of pig by higher concentration at diseased region, to the treatment of disease more effectively, definite; Targeting preparation reduces medicine to upper gastrointestinal stimulation, and bad reaction is few, easy to use, and compliance is good. Make the breeding enterprise of pig obtain good economic benefit.
Description
Technical field
The present invention relates to a kind of liquid, particularly, relate to a kind of endometritis and colpitic perfusion liquid for the treatment of pig.
Background technology
The vaginitis of pig, endometritis are a kind of common Main Reproductive Organs disease in Swine Production, most absolutelyThe sick pigs of number are external intrusion pathogen and infecting, and during as childbirth, injury of birth canal is polluted, and afterbirth fragment is remaining, lochia when atony of uterusBe detained, artificial insemination sterilization is not tight, has rheuminess thing etc. when natural mating in boar reproductive organs or seminal fluid. Its cause of disease has twoCoccus, Streptococcusagalactiae, staphylococcus aureus and Escherichia coli, how per vaginam scorching and then develop into endometritis. According to facingBed statistics, oestrus of sow is undesired or postpone, and the sow of Repeat breeding more than 80% is trouble endometritis, can give like this foster motherHeavy economic loss is brought at pig family. High, the difficult control of the incidence of disease clinically of this disease, causes huge economy to the aquaculture of pigLoss.
Endometritis, the vaginitis prophylactico-therapeutic measures of pig is at present a lot, as anti-inflammation, enhancing Abwehrkraft des Koepers, and withVaccine immunity and good management raising etc., but the endometritis of pig, vaginitis incidence probability are still high especially. ParticularlyFirst endometritis, the vaginitis of the clinical antibiotic generally adopting and chemical drug treatment pig are endometritis, the vagina of pigScorching clinical symptom disappearance, but be easy to recurrence; Secondly antibiotic and the antibacterial drug resistance ubiquity of chemical drug, uses antibioticContinuing to increase with the dosage of chemical drug, but clinical efficacy is bad, adds antibiotic residue, directly harm humans health.
Sophora alopecuroide is perennial leguminous herbaceous plant, dry herb and the seed of Sophora alopecuroide. Sophora alopecuroide is distributed widely in sweetThe ground such as respectful, Ningxia, Xinjiang, Tibet, the Inner Mongol. Herb is gathered summer, and seed is gathered spring, dry. The raw use of herb, seed is friedWith. Country lists Sophora alopecuroide in one of six large authentic medicinal herbses of focused protection in recent years, includes national modernization of Chinese medicine science and technology in and producesIn industry action plan, there is significant progress for construction of production base and deep level development.
Sophora alopecuroide taste is extremely bitter, cold in nature poisonous, has the effects such as clearing heat and detoxicating, anti-inflammation, pain relieving desinsection. Among the people with radical cureTreat laryngalgia, cough, dysentery and eczema etc. The current 8 kinds of alkaloids that mainly contain for drug development: sophocarpine, matrine, oxidationMatrine, N-Oxysophocarpine one, sophoramine, Sophoridine, lehmannine, aloperine.
The pharmacological action of Alkaloids From Sophora Alopecuroides L: 1, antibacterial action, successfully develop at present treatment dysentery medicine Sophora alopecuroideSheet and treatment gynaecological imflammation medicine anti-inflammatory suppository for gynecopathy (Sophora alopecuroide) bolt; 2, the effect to cardiovascular system, is embodied in anti-arrhythmia, positivityMyodyamia effect, step-down and reduction heart rate function, to the protective effect of myocardial ischemia and infraction, effect for reducing blood fat; 3, antiviral, anti-Inflammation and immunosuppressive action, sophora alapecuroides can suppress multiple virus breeding. strengthen immunoloregulation function and immunologic enhancement;4, antitumor action, obvious to HCC inhibitory action, cervical carcinoma, lymphosarcoma, lung cancer are also had to inhibitory action; 5, to flatThe effect of sliding flesh, sophora alapecuroides and some monosomic alkali all can resist the Contraction of The Ileum From A White of being induced by inflammatory mediator, rightBronchial smooth muscle has relexation, and block mold is also had to effect: 6, current clinical practice Sophora alopecuroide medicinal extract sheet, aloperineTreatment acute bacillary dysentery, sophocarpine sheet treatment bronchial astehma, Sophorcarpidine Treating gynaecological imflammation, Treated by Oxymatrine Injection chronicType virus hepatitis all obtains good curative effect.
Summary of the invention
The invention provides a kind of endometritis and colpitic perfusion liquid for the treatment of pig, is taking sophora alapecuroides as formerMaterial, has good result for the treatment of, and is difficult for recurrence; Because what use is the autonomic drug of pure natural, so also there is not anti-medicineThe problem of property. The present invention also provides the preparation method and application of this perfusion liquid.
The invention provides a kind of endometritis and colpitic perfusion liquid for the treatment of pig, effective one-tenth of described perfusion liquidBe divided into sophora alapecuroides.
Preferably, the sophora alapecuroides that described perfusion liquid contains mass percent 3-8%.
Preferably, described perfusion liquid is prepared from by following steps:
1) prepare sophora alapecuroides: get Sophora alopecuroide seed meal and be broken to 50-80 order, adding percent by volume is 50%-90%'sEthanol water carries out ultrasonic wave extraction, supersonic frequency 20KHz, and 5000W, reclaims ethanol after filtering, and adds 2% hydrochloric acid to adjust pH to be2-5, adds ether washing 2 times, obtains filtrate precipitation, then adds three precipitations of chloroform extraction, and recovered under reduced pressure chloroform obtains bitter beansSub-total alkali;
2) prepare perfusion liquid: in the sophora alapecuroides obtaining in step 1), add water for injection constant volume, sophora alapecuroides andThe mass ratio of water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, carry out filling under aseptic conditionObtain perfusion liquid.
The present invention also provides a kind of endometritis of pig and preparation method of colpitic perfusion liquid for the treatment of, step asUnder:
1) prepare sophora alapecuroides: get Sophora alopecuroide seed meal and be broken to 50-80 order, adding percent by volume is 50%-90%'sEthanol water carries out ultrasonic wave extraction, supersonic frequency 20KHz, and 5000W, reclaims ethanol after filtering, and adds 2% hydrochloric acid to adjust pH to be2-5, adds ether washing 2 times, obtains filtrate precipitation, then adds three precipitations of chloroform extraction, and recovered under reduced pressure chloroform obtains bitter beansSub-total alkali;
2) prepare perfusion liquid: in the sophora alapecuroides obtaining in step 1), add water for injection constant volume, sophora alapecuroides andThe mass ratio of water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, carry out filling under aseptic conditionObtain perfusion liquid.
Wherein, sophora alapecuroides can be selected commercially available product.
The 3rd object of the present invention is to provide a kind of preparation method of sophora alapecuroides, and step is as follows: get Sophora alopecuroide kindSon is crushed to 50-80 order, and adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic wave extraction, supersonic frequency20KHz, 5000W, reclaims ethanol after filtering, and adding 2% hydrochloric acid to adjust pH is 2-5, adds ether washing 2 times, obtains filtrate precipitation,Add three precipitations of chloroform extraction, recovered under reduced pressure chloroform obtains sophora alapecuroides again.
The 4th object of the present invention be to provide sophora alapecuroides in the treatment endometritis of pig and vaginitis shouldWith.
The 5th object of the present invention is to provide endometritis and the vaginitis of sophora alapecuroides perfusion liquid treatment pigIn application.
Preferably, in described sophora alapecuroides perfusion liquid, the quality percentage composition of sophora alapecuroides is 3-8%.
Preferably, be that sophora alapecuroides perfusion liquid is filled in the uterus of pig, 100 milliliters of each consumptions, once a day.
That perfusion liquid of the present invention has is antibacterial, anti-inflammatory, low toxicity, noresidue, be difficult for producing the advantages such as drug resistance, and stimulatesProperty is little, and cost is lower, can effectively treat the disease such as endometritis, vaginitis of the pig causing because of bacterium, fungi, trichomonad,The particularly endometritis of targeted therapy pig, vaginitis. The present invention has the extract of single medicinal material, is conducive to the steady of productQualitative and quality controllability; Targeting preparation discharges in the endometrium of pig and vagina, reduces gastral absorption, at diseased regionDirectly discharge and can be scattered in the endometrium and vagina of pig by higher concentration, to the treatment of disease more effectively, definitely; TargetPreparation reduces medicine to upper gastrointestinal stimulation, and bad reaction is few, easy to use, and compliance is good. The breeding enterprise of pig is obtainedGood economic benefit.
Detailed description of the invention
Following embodiment is convenient to understand better the present invention, but does not limit the present invention. Experiment in following embodimentMethod, if no special instructions, is conventional method. The sophora alapecuroides using in following embodiment, also can select commercially available,Its effect is identical with the homemade sophora alapecuroides of the applicant.
Embodiment mono-Oxford agar diffusion method is measured the antibacterial activity in vitro of sophora alapecuroides
Adopt Oxford agar diffusion method to measure sophora alapecuroides to staphylococcus aureus, Escherichia coli, Streptococcusagalactiae, two ballThe antibacterial activity of bacterium. Result of the test shows, when sophora alapecuroides concentration is 5mg/ml, staphylococcus aureus, Streptococcusagalactiae,Diplococcus, colibacillary antibacterial circle diameter are respectively 10.05mm, 6.89mm, 10.66mm, 9.94mm. In the time of 20mg/ml, press downBacterium loop diameter is respectively 13.98mm, 12.93mm, 19.61mm, 19.30mm. Can find out the rising along with concentration, Sophora alopecuroide is totalAlkali to Escherichia coli and diplococcal inhibitory action apparently higher than to staphylococcus aureus and Streptococcusagalactiae. Wherein to two ballsThe inhibitory action of bacterium is the strongest, and to the inhibitory action of Streptococcusagalactiae, other three kinds of bacterium are the most weak relatively.
1 materials and methods
1.1 materials and reagent
For trying bacterium: staphylococcus aureus, Escherichia coli, Streptococcusagalactiae, diplococcus, grind by Lanzhou herding and veterinary drugJiu Suo veterinary drug research department provides.
Supply sample: sophora alapecuroides, produced preparation method and embodiment bis-by Hinapharm Pharmaceutical Co., Ltd.In identical, after dissolving with water for injection, be mixed with the test liquid of variable concentrations.
1.2 instrument and equipment
KH-300DB type numerical control ultrasonic cleaner, Kunshan He Chuan ultrasonic instrument Co., Ltd;
RE-201D Rotary Evaporators, Yuhua Instrument Co., Ltd., Gongyi City;
Electro-heating standing-temperature cultivator, the Shanghai DNP-9272A of Hong Dou Electronic Science and Technology Co., Ltd. type;
Constant temperature oscillator, the IS-RDV1 of Jing Qi Co., Ltd of the U.S.;
Biohazard Safety Equipment, the HFsafe-1500 of Shanghai Shen Li scientific instrument Co., Ltd.
The preparation of 1.3 bacteria suspensions
Bacterium liquid good activation culture is accessed respectively in SPSS, carry out successively gradient dilution, make in bacteria suspensionBacterial population be 107cfu/ml。
1.4 Oxford agar diffusion methods are measured antibacterial activity
The culture medium 20ml having melted is added in the culture dish that autoclaving crosses, solidify rear absorption 200 microlitre bacteria suspensionsAdd wherein, by even the coating of bacterium liquid, make the plate that carries disease germs with spreader, with aseptic nipper gripping high pressure steam sterilization bakingDry Oxford cup, is flat on the culture dish surface that has been coated with bacterium liquid gently, 4 Oxford cups of the symmetrical placement of each culture dish cross,The confession sample that pipettes respectively 200 microlitre different quality concentration with aseptic liquid-transfering gun, in the cup of Oxford, slowly moves into perseverance by culture dishTemperature incubator, cultivates 18h for 37 DEG C, and take out and observe each culture dish colony growth situation, and measure antibacterial circle diameter with slide measure,Record data, are respectively 5mg/ml, 10mg/ml, 15mg/ml, 20mg/ml for the concentration of sample.
2 results
Measure by above method, result is as following table 1.
。
3 conclusions
Endometritis, the colpitic main several pathogenic bacteria of sophora alapecuroides to pig all have inhibitory action, and oneRising inhibitory action along with sophora alapecuroides concentration in fixed scope also progressively strengthens, wherein to diplococcal inhibitory actionBy force, to the inhibitory action of Streptococcusagalactiae, other three kinds of bacterium are the most weak relatively. Sophora alapecuroides can further be studied thin to otherThe inhibitory action of bacterium, for the exploitation of sophora alapecuroides provide reference frame.
Embodiment bis-: sophora alapecuroides is studied acute toxicity test in mice
Adopt improvement karber's method to measure the acute toxicity of sophora alapecuroides to mouse peritoneal injection, evaluate its security, forData for clinical drug use provides foundation. When test, give the sophora alapecuroides of test group mouse gavaging variable concentrations, little after observation administrationThe activity of mouse and death condition, adopt improvement Kou Shi formula to calculate LD50And LD5095% fiducial limit, result shows, Sophora alopecuroide is totalAlkali is through the LD of lumbar injection50For 559.24mg/kg, LD50The 95% credible 471.95mg/kg～663.59ma/kg that is limited to. TestResult is tentatively pointed out, and the toxicity of sophora alapecuroides is lower, and data for clinical drug use is reliable.
1 materials and methods
1.1 material
1.1.1 experimental animal
Kunming system clean level healthy mice, male and female half and half, body weight (20 ± 2) g, purchased from Lanzhou University's experimental animal center(sweet doctor moves word 14-005).
1.1.2 tested medicine
Sophora alapecuroides, extracts preparation by Hinapharm Pharmaceutical Co., Ltd..
1.2 method
1.2.1 the extraction of sophora alapecuroides
Get Sophora alopecuroide seed meal and be broken to 50-80 order, adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonicRipple extracts, supersonic frequency 20KHz, and 5000W, reclaims ethanol after filtering, and adding 2% hydrochloric acid to adjust pH is 2-5, adds ether washing 2Inferior, obtain filtrate precipitation, then add three precipitations of chloroform extraction, recovered under reduced pressure chloroform obtains sophora alapecuroides, after testing, bitter beansThe purity of sub-total alkali is 98%, and detecting instrument is that high-efficient liquid phase color spectrometer detects.
Detection method is as follows: 6% sophora alapecuroides perfusion liquid (in sophora alapecuroides); Sophoridine reference substance (lot number:11060901, content >=98%), sophocarpine reference substance (lot number: 11052422, content >=98%) is purchased from the biological section of Chengdu Man SiteSkill Co., Ltd; Matrine reference substance (lot number: 110805-200508) is purchased from Nat'l Pharmaceutical & Biological Products Control Institute; Acetonitrile isChromatographically pure, purchased from Shandong Chemical Co., Ltd. in pairs, it is pure that other reagent are analysis. Chromatographic column: YMC-packedODS-A post(150mm × 4.6mm, m), mobile phase is V1(acetonitrile to 5 μ): V2(0.05mol/LKH2PO3): V3(triethylamine)=8: 92: 0.18,Detect wavelength 220nm, under the condition of flow velocity 1.0mL/min, detect.
1.2.2 trial test
Before trial test, mouse is carried out the nursing observation of 7d, in the observation period, mouse free choice feeding, drinking-water, eliminates natural deathMouse dies. In the trial test phase, choose 25 of healthy mices, male and female half and half, are divided into 5 groups at random. Every group 5, before test, fasting 8 is littleTime, sophora alapecuroides is mixed with to 5 concentration, respectively organize isoconcentration intraperitoneal injection, determine the dosage range of formal test.
1.2.3 formal test
According to trial test, determine Dn and Dm, between Dn and Dm, establish 6 dosage groups, every group of 10 mouse, male and female half and half.Before test, fasting 8 hours, freely drinks water. After intraperitoneal injection, observe immediately and record behavior state and the dead feelings of mouseCondition, the observation period is 7 days, calculates median lethal dose (LD according to experimental result50), dead mouse is dissected in time, record pathology feelingsCondition, observes mouse peritoneal and parenchymatous organ's pathological change.
1.2.4LD50Computational methods
Adopt improvement karber's method to calculate LD50And LD5095% fiducial limit, computing formula is as follows:
LD50=lg-1[Xm-?(Σp-0.5)]
Sx50=?
LD5095% fiducial limit=lg-1（lgLD50±1.96×Sx50）
In formula: be group distance, the log10 dose of adjacent 2 groups of dosage is poor; Xm is maximum death rate group dosage logarithm; Σ pFor each dosage group death rate sum; P is each dosage group death rate; Q is each dosage group survival rate; N is each treated animal number; Sx50ForlgLD50Standard error.
2 results and analysis
2.1 trial test results
Sophora alapecuroides is as shown in table 2 to chmice acute toxicity trial test result. Drawn by trial test result, Dn and Dm divideWei 200mg/kg and 1000mg/kg. Determine that according to trial test result formal test is divided into 6 dosage groups, obtains agent by following formulaAmount ratio:
r=(N-1)
In formula, r is the ratio of two adjacent groups dosage; N is required group of number of formal test. Calculate the r=of formal test0.707。
。
According to trial test result, be made as 200mg/kg, 276.24mg/kg, 381.55mg/kg, 527.00mg/kg,727.90mg/kg, 6 dosage groups of 1000mg/kg body weight, carry out acute toxicity test. Result is as shown in table 3. According to table 3In data, calculated the median lethal dose LD of sophora alapecuroides to mouse by formula50=559.24mg/kg，LD5095% credibleBe limited to 471.95mg/kg～663.59mg/kg. Therefore judge sophora alapecuroides to mouse through lumbar injection LD50Be greater than 500mg/Kg, according to China's animal toxicology acute toxicity staging, sophora alapecuroides belongs to low toxicity material.
。
2.3 sophora alapecuroides is to acute toxicity test in mice clinical observation
Mouse disposable celiac drug administration by injection, when dosage is 200mg/kg, within experimental period, mouse is without the phenomena of mortality, and 2 is littleTime not dead mouse symptom alleviate gradually, substantially after 12 hours recover normal, can normally take food and drink water; Dosage isWhen 276.24mg/kg, one of dead mouse in 1 hour; When dosage is 381.55mg/kg, dead mouse 2 within 15 minutesOnly; When dosage is 527.00mg/kg, 3 of dead mouses in 10 minutes; When dosage is 727.90mg/kg, little in 10 minutesDead 6 of mouse; When dosage is 1000mg/kg, in 5 minutes, mouse is all dead.
The main poisoning symptom of mouse after intraperitoneal injection shows as One's spirits are drooping, hogback, and whole body is twitched, and fore paw is scratchedMouth, and have discontinuity excitement. On one's deathbed before, present jump action, four limbs are swimming shape then more, dead subsequently.
The dead mouse of 2.4 acute toxicity tests is cutd open inspection result
Dead mouse is dissected and finds there is a small amount of hydrops in abdominal cavity, heart color and luster dimness, liver has slight enlargement, kidneyObserve without significant change, other organs is without obviously observing pathology. Survival mice and blank group mouse were put to death and separated after 6 daysCut open observation, do not find seroperitoneum, other organs is without obviously observing pathology.
3 results
Result of the test shows, the median lethal dose LD of sophora alapecuroides to mouse peritoneal injection50For 559.24mg/kg,LD5095% the credible 471.95mg/kg～663.59ma/kg that is limited to, meet low toxicity material grade scale.
After disposable mouse peritoneal drug administration by injection, survival mice is basic after 12 hours recovers normal, after 6 days to survival miceCarry out Anatomical Observation, each internal organs are showed no obviously observes pathology. After test is described, survival mice recovers very fast, and medicine is held itContinuous impact is less.
The research of sophora alapecuroides on mankind's clinical medicine is more with application, but research aspect animal husbandry production withApply lessly, this test is evaluated by the acute toxicity to sophora alapecuroides, for sophora alapecuroides is later in animal husbandryIn further developmental research should be used as preparation.
Endometritis, the vaginitis of embodiment 3 use sophora alapecuroides perfusion liquid treatments pigs, effect is clear and definite, after healing notEasily bounce-back.
From 2011 to 2012 Nian Wo companies, endometritis, the vaginitis of sophora alapecuroides perfusion liquid treatment pig are facedBed test. Successively select trouble endometritis, colpitic sow as subjects on pig farm, silver Jingyuan through consultation.
Wherein, suffer from 10 of chronic endometritis, colpitic sows, it is normal that clinical symptoms show as all feelings of oestrusing, repeatlyJoin infertilely, even pregnant, great majority are also miscarried, and discharge the muddy mucus of floccule from vaginal orifice, row when particularly oestrusing or keeping downGo out more. When serious, discharged by vaginal orifice, with canescence or filemot purulent exudate or the purulence of foul smell. Some appetite subtractsMove back or give up absolutely, hyperthermia.
Suffer from 10 of acute endometritis, vaginitis sows, clinical symptoms show as mucus and mucus purulence. Sick pig is completeBody symptom is obvious, and spirit is depressed, and appetite declines even absolutely useless, and hyperthermia, is generally 39.5 DEG C～41.5 DEG C, hogback, and frequent micturition,Exert duty, constantly discharge bois de rose or yellow-white mucus frowzy or purulent secretion from vaginal orifice, it is dirty red that severe one isOr brown, in the time that it is droped to the ground, discharge morely, be often mixed with afterbirth fragment.
1. test medicine
Sophora alapecuroides perfusion liquid: wherein the content of sophora alapecuroides is 6%(mass percent).
Injection penicillin is purchased from local veterinary drug shop, and by expedition, pharmaceutcal corporation, Ltd produces, and lot number is 20110623, specificationFor 5g/ props up.
2. test
2.1 test groupings
5 to 19 June in 2011, on this pig farm using 10 ill sows that occurred clinical symptoms as first group; 6There were 22 ill sows that occur clinical symptoms the moon on 25th in same pasture to July 25, select 10 and first group of situation baseThis similar ill sow is as second group; Every group include 5 chronic endometritis, colpitic sow and 5 acuteEndometritis, colpitic sow, to two groups, administration sophora alapecuroides perfusion liquid, injection ampicillin sodium carry out respectively respectivelyTreatment.
2.2 test method
Test group adopts sophora alapecuroides perfusion liquid to treat, every sow potion, by semen deposition bottle or enema syringe orSyringe (100ml) connection is about the simple and easy vas deferens of 50cm or stomach catheter carries out perfusion in uterus, and 100 milliliters of each consumptions are everyIt once, being used in conjunction 5d is a course for the treatment of; Control group adopts Ampicillin Sodium For Injection, the each 50ml of uterine perfusion, once a day,Being used in conjunction 5d is a course for the treatment of.
3 results
After medication, cure and show as the normal purulent secretion of discharging from vaginal orifice, gray, yellowish-brown, canescence secretion disappearLose, on vaginal orifice surrounding skin and root of the tail, adhering to purulent secretion and disappearing. In uterus with sophora alapecuroides perfusion liquid treatment pigFilm inflammation, colpitic cure rate are 80%, and endometritis, the colpitic cure rate of ampicillin sodium treatment pig are 70%.
10 sows of sophora alapecuroides perfusion liquid group have endometritis, the vaginitis of 8 sows after medication 5dDisappear or obviously change, cure rate is 80%(8/10), endometritis, vaginitis do not recur. 10 of ampicillin sodium groupSow has 7 endometritises, vaginitis symptom disappear or obviously change after medication after five days, cure rate is 70%(7/10), in healing process, there are endometritis, vaginitis rebound phenomenon, cure not thorough. Controlling of sophora alapecuroides perfusion liquid groupTherapeutic effect is higher than ampicillin sodium group.
4 conclusions
Sophora alapecuroides perfusion liquid, Ampicillin Sodium For Injection treatment Gilt Uterus intimitis, vaginitis are respectively 87.5%With 78%, aspect cure rate, sophora alapecuroides perfusion liquid is higher. Aspect economic benefit, Ampicillin Sodium For Injection is in treatmentFrequent use during this time easily causes drug resistance, causes again treating more difficult; Sophora alapecuroides perfusion liquid is as pure natural medicineThing, has toxic and side effect little, and the advantage such as be difficult for developing immunity to drugs, can save cost significantly, and herbal treatment focuses on conditioning,Be conducive to the recovery of animal economy function and body condition. Therefore sophora alapecuroides perfusion liquid treatment Gilt Uterus intimitis, vaginitisThere is vast potential for future development.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention,Although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, it still canSo that the technical scheme that aforementioned each embodiment was recorded is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., all should be included in of the present inventionWithin protection domain.
Claims (5)
1. the application of sophora alapecuroides in bacillary endometritis and the colpitic medicine of preparation treatment pig, described bacteriumFor diplococcus.
2. the application of sophora alapecuroides perfusion liquid in bacillary endometritis and the colpitic medicine of preparation treatment pig, instituteStating bacterium is diplococcus.
3. application according to claim 2, is characterized in that: the preparation method of described sophora alapecuroides perfusion liquid is as follows:
1) prepare sophora alapecuroides: get Sophora alopecuroide seed meal and be broken to 50-80 order, adding percent by volume is the second of 50%-90%Alcohol solution carries out ultrasonic wave extraction, supersonic frequency 20KHz, and 5000W, reclaims ethanol after filtering, and adds 2% hydrochloric acid to adjust pH to be2-5, adds ether washing 2 times, obtains filtrate precipitation, then adds three precipitations of chloroform extraction, and recovered under reduced pressure chloroform obtains bitter beansSub-total alkali;
2) prepare perfusion liquid: in the sophora alapecuroides obtaining in step 1), add water for injection constant volume, sophora alapecuroides and injectionThe mass ratio of water is (3-8): (92-97), adopt flowing steam sterilization 30-40min, carry out filling obtaining under aseptic conditionTo perfusion liquid.
4. according to the application described in claim 2 or 3, it is characterized in that: sophora alapecuroides in described sophora alapecuroides perfusion liquidQuality percentage composition be 3-8%.
5. according to the application described in claim 2 or 3, it is characterized in that: each consumption of described sophora alapecuroides perfusion liquid is100 milliliters, once a day.
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|---|---|---|---|---|
| CN102100734A (en) * | 2009-12-18 | 2011-06-22 | 武新安 | Sophora alopecuroide total alkali medicine for treating herpes zoster |
| CN103142683A (en) * | 2013-03-12 | 2013-06-12 | 广东海纳川药业股份有限公司 | Sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis as well as preparation method of perfusion fluid |
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| CN103271983A (en) | 2013-09-04 |
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