CN103142683A - Sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis as well as preparation method of perfusion fluid - Google Patents
Sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis as well as preparation method of perfusion fluid Download PDFInfo
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Abstract
The invention discloses a sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid, which is quick in becoming effective, low in toxicity, easy in metabolism and free of residues and is used for treating bovine mastitis and endometritis as well as a preparation method of the perfusion fluid. The sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis has the beneficial effects of being small in toxic or side effect and unlikely to generate resistance to drugs by adopting the sophora alopecuroide total alkaloid perfusion fluid as the pure natural medicine, as well as remarkably saving the cost and reducing abandoned milk loss; and moreover, traditional Chinese medicine therapy lays emphasis on nursing and is beneficial to recovering the integral function and body condition. The sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid has an extensive development prospect in treating the bovine mastitis.
Description
Technical field
The present invention relates to the Chinese medicine application, be specifically related to be used for the treatment of the Chinese medicinal perfusion liquid that contains Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis.
Background technology
Herba Sophorae alopecuroidis is perennial leguminous herbaceous plant, dry herb and the seed of Herba Sophorae alopecuroidis.Herba Sophorae alopecuroidis is distributed widely in the ground such as Gansu, Ningxia, Xinjiang, Tibet, the Inner Mongol.Herb is gathered summer, and seed is gathered spring, drying.Herb is given birth to use, and seed is fried and used.Country lists Herba Sophorae alopecuroidis in one of six large authentic medicinal herbses of focused protection in recent years, includes in national modernization of Chinese medicine scientific and technological industry action plan, for construction of production base and deep level development, significant progress is arranged.The Herba Sophorae alopecuroidis flavor is extremely bitter, cold in nature poisonous, has heat-clearing and toxic substances removing, the effects such as anti-inflammation, pain relieving parasite killing.Among the people with radical cure treatment laryngalgia, cough, dysentery and eczema etc.The 8 kinds of alkaloids that mainly contain that are used at present drug development: sophocarpine, matrine, oxymatrine, N-oxysophocarpine one, sophoramine, sophoridine, lehmannine, aloperine.The pharmacological action of Alkaloids From Sophora Alopecuroides L: 1, antibacterial action, successfully developed at present treatment dysentery medicine Herba Sophorae alopecuroidis sheet and treatment gynecological inflammation medicine anti-inflammatory suppository for gynecopathy (Herba Sophorae alopecuroidis) bolt; 2, to the effect of cardiovascular system, be embodied in arrhythmia, positive inotropic action, blood pressure lowering and reduction heart rate function are to the protective effect of myocardial ischemia and infraction, effect for reducing blood fat; 3, antiviral, antiinflammatory and immunosuppressive action, Herba Sophorae alopecuroidis total alkali can suppress multiple virus breeding. strengthen immunoloregulation function and immunologic enhancement; 4, antitumor action, obvious to the hepatoma carcinoma cell inhibitory action, cervical cancer, lymphosarcoma, pulmonary carcinoma also there are inhibitory action; 5, to the effect of smooth muscle, Herba Sophorae alopecuroidis total alkali and some monosomic alkali all can resist the Contraction of The Ileum From A White of being induced by inflammatory mediator, bronchial smooth muscle had relexation, block mold is also had effect: 6, clinical practice Herba Sophorae alopecuroidis extractum sheet, aloperine are treated acute bacillary dysentery at present, sophocarpine sheet treatment bronchial asthma, Sophorcarpidine Treating gynecological inflammation, Treated by Oxymatrine Injection chronic viral hepatitis B are all obtained good curative effect.
Bovine mastitis and endometritis are a kind of diseases of the most common in world's dairy and economic loss maximum, and its pathogenic factor is mainly cause pathogeny imcrobe infection.The pathogenic microorganism that causes bovine mastitis and endometritis is very complicated, comprises antibacterial, fungus, virus, mycoplasma etc.Wherein take the antibacterial infection as main, mainly contain staphylococcus aureus, escherichia coli, streptococcus agalactiae, staphylococcus epidermidis.Next is fungal infection, and usually mixed infection.The generation of this disease directly affects the Quality and yield of milk, also can reduce the time limit of giving milk of milch cow, has increased mortality.High, the difficult control of sickness rate clinically of this disease causes huge economic loss for the milk cattle cultivating industry.
At present bovine mastitis and endometritis prophylactico-therapeutic measures is a lot, as anti-inflammation, enhancing body resistance, and with vaccine immunity and good management raising etc., but bovine mastitis and endometritis incidence probability are still high especially.Particularly the clinical antibiotic that generally adopts and chemical medicine treatment bovine mastitis and endometritis, be at first the clinical symptom disappearance of bovine mastitis and endometritis, but be easy to recurrence; Secondly antibiotic and the antibiotic drug resistance ubiquity of chemical medicine, use the dosage of antibiotic and chemical medicine continuing to increase, but clinical efficacy is bad, adds antibiotic remains, and directly harm humans is healthy.
Summary of the invention
Purpose of the present invention is exactly for above-mentioned defective of the prior art, and a kind of milch cow pathogenicity mastitis and endometritis of being used for the treatment of is provided, and produce effects is fast, toxicity is low, the Chinese medicinal perfusion liquid of easy metabolism, noresidue.
To achieve these goals, technical scheme provided by the invention is: a kind of Chinese medicinal perfusion liquid, contain Herba Sophorae alopecuroidis total alkali in described Chinese medicinal perfusion liquid.
Further, above-mentioned Chinese medicinal perfusion liquid contains Herba Sophorae alopecuroidis total alkali 3-8g in described Chinese medicinal perfusion liquid.
Further, above-mentioned Chinese medicinal perfusion liquid contains Herba Sophorae alopecuroidis total alkali 6g in described Chinese medicinal perfusion liquid.
Second purpose of the present invention has been to provide a kind of preparation method of Chinese medicinal perfusion liquid, comprises the following steps:
1) get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W reclaims ethanol after filtering, and adding 2% hydrochloric acid to transfer pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, then add three precipitations of chloroform extraction, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add the water for injection standardize solution in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, carry out fill and get finished product Chinese medicinal perfusion liquid under aseptic condition.
Further, above-mentioned preparation method, described step 2) in, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is 6:94.
The 3rd purpose of the present invention has been to provide Herba Sophorae alopecuroidis total alkali for the preparation of the application in the Chinese medicinal perfusion liquid for the treatment of bovine mastitis and endometritis.
Further, above-mentioned application, the use amount of described Chinese medicinal perfusion liquid is each breast district 30-60ml of every cow head.
Beneficial effect of the present invention is: the Chinese medicinal perfusion liquid that contains Herba Sophorae alopecuroidis total alkali provided by the invention, to compare with Ampicillin Sodium For Injection, and the cure rate for the treatment of mammitis of cow is respectively 90% and 70%, the Herba Sophorae alopecuroidis total alkali infusion liquid is higher aspect cure rate.Aspect economic benefit, add the withdrawal time of 4 days during the Ampicillin Sodium For Injection treatment, abandoning the milk phase of nearly 7d arranged, the residual health that affects consumer of medicine, frequent use easily causes drug resistance, causes retreatment more difficult; The Herba Sophorae alopecuroidis total alkali infusion liquid has toxic and side effects little as pure natural medical, and the advantage such as be difficult for developing immunity to drugs can be saved cost significantly, reduce and abandon the milk loss, and herbal treatment pays attention to conditioning, is conducive to the recovery of animal economy function and body condition.The Chinese medicinal perfusion liquid that this kind contains Herba Sophorae alopecuroidis total alkali has vast potential for future development for the treatment bovine mastitis.The preparation method of Herba Sophorae alopecuroidis total alkali infusion liquid provided by the invention, process is simple.The invention also discloses the application of extract Herba Sophorae alopecuroidis total alkali infusion liquid in treatment bovine mastitis and endometritis of Herba Sophorae alopecuroidis, that this medicine has is antibiotic, antiinflammatory, low toxicity, noresidue, be difficult for producing the advantage such as drug resistance, and zest is little, cost is lower, can effectively treat control and the treatment, particularly targeted therapy bovine mastitis and endometritis of the diseases such as the bovine mastitis that causes because of antibacterial, fungus, infusorian and endometritis.The present invention has the extract of single medicinal material, is conducive to stability and the quality controllability of product; Targeting preparation discharges at cow mammary gland and intrauterine, reduces gastral absorption, directly discharges and can be scattered in cow breast and uterus by higher concentration at diseased region, and is more effective, definite to the treatment of disease; Targeting preparation reduces medicine to upper gastrointestinal stimulation, and untoward reaction is few, and is easy to use, and compliance is good.Make dairy farmer and the good economic benefit of milk cattle cultivating enterprise's acquisition.
The concrete testing result that contains the Chinese medicinal perfusion liquid of Herba Sophorae alopecuroidis total alkali provided by the invention:
One, the mensuration of Herba Sophorae alopecuroidis total bases extract
Herba Sophorae alopecuroidis total alkali, get approximately 0.15g of this product, accurately weighed, put in beaker, add hydrochloric acid 0.2mol/L5ml dissolving, move in separatory funnel, water 10ml divides several to wash beaker, washing liquid and solution merge, add sodium chloride 6g, shake loose, add again caustic lye of soda 1mol/l5ml, extract with the chloroform jolting and be respectively 25ml 5 times, 20ml, 10ml, 10ml, 10ml divides the chloroform layer got successively by being plugged with absorbent cotton and filling the glass funnel of 1g anhydrous sodium sulfate, filter enters in another separatory funnel, combined chloroform liquid, precision adds sulphuric acid volumetric solution 0.05mol/L20ml and water 10ml.After jolting is extracted, tell the acid layer, chloroform layer washes with water three times, each 10ml.Merge acid solution and washing liquid, put in conical flask, heating in water bath is removed residual chloroform, let cool to room temperature, and 2 of the red indicators of methylate, with sodium hydroxide volumetric solution 0.1mol/l titration, every 1ml sulphuric acid volumetric solution 0.05mol/l is equivalent to the C1 of 24.84mg
5H
21N
2O。
Sophoridine is measured according to two appendix VID of high performance liquid chromatography Chinese Pharmacopoeia version in 2000.
Chromatographic condition and system suitability: chromatographic column, Spherisorb nh 2 column; Take acetonitrile-phosphate aqueous solution PH2-dehydrated alcohol 80:8:10 as mobile phase; Detect wavelength 220nm; The separating degree of sophoridine and related alkaloids should meet the requirements; The relative standard deviation should be less than 2.0%.
Algoscopy: get this product 25mg, accurately weighed, put in the 100ml measuring bottle, dissolve and be diluted to scale with acetonitrile-dehydrated alcohol 8:1, shake up.Precision measures 20 μ l injection liquid chromatographies, records chromatogram; Separately get approximately 10mg of sophoridine reference substance, accurately weighed, put in the 100ml measuring bottle, with above-mentioned dissolution with solvents and be diluted to scale, shake up, measure with method.Go out sophoridine C in test sample by external standard method with calculated by peak area
15H
24N
2The content of O.
Two, concrete experimentation:
(1) the Oxford agar diffusion method is measured the antibacterial activity in vitro of total alkaloid of sophora alopecuroide
Adopt the Oxford agar diffusion method to measure the antibacterial activity of Herba Sophorae alopecuroidis total alkali to escherichia coli, staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae.Result of the test shows, when Herba Sophorae alopecuroidis total alkali concentration is 5mg/ml, the antibacterial circle diameter of escherichia coli, staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae is respectively 9.94mm, 10.65mm, 10.66mm, 6.89 mm.Antibacterial circle diameter is respectively 19.30 mm, 13.98 mm, 19.69 mm, 12.92 mm when 20mg/ml.Can find out the rising along with concentration, Herba Sophorae alopecuroidis total alkali to the inhibitory action of escherichia coli and staphylococcus epidermidis apparently higher than to staphylococcus aureus and streptococcus agalactiae.Wherein the strongest to the staphylococcic inhibitory action of epidermis, the most weak to relative other the three kinds of bacterium of the inhibitory action of streptococcus agalactiae.
1, materials and methods
1.1, material and reagent
For the examination bacterium: staphylococcus aureus, escherichia coli, streptococcus agalactiae, staphylococcus epidermidis provide by Lanzhou herding and veterinary drug institute veterinary drug research department.
Supply sample: Herba Sophorae alopecuroidis total alkali, produced by Hinapharm Pharmaceutical Co., Ltd., with the test liquid that is mixed with variable concentrations after water dissolution.
1.2, instrument and equipment
KH-300DB type numerical control ultrasonic cleaner, Kunshan standing grain wound ultrasonic instrument company limited; The RE-201D Rotary Evaporators, Yuhua Instrument Co., Ltd., Gongyi City; Electro-heating standing-temperature cultivator, Shanghai letter be electronics technology company limited DNP-9272A type all; Constant temperature oscillator, U.S. essence black horse company limited IS-RDV1; Biohazard Safety Equipment, Shen, Shanghai power scientific instrument company limited HFsafe-1500.
1.3, the preparation of bacteria suspension
The bacterium liquid that activation culture is good accesses respectively in physiological saline solution, carries out successively gradient dilution, and making the bacterial population in bacteria suspension is 107cfu/ml.
1.4, the Oxford agar diffusion method measures antibacterial activity
the culture medium 20ml that has melted is added in the culture dish that autoclaving crosses, solidifying rear absorption 200 microlitre bacteria suspensions adds wherein, with spreader with the coating of bacterium liquid evenly, make the plate that carries disease germs, Oxford cup with aseptic nipper gripping high pressure steam sterilization and oven dry, be flat on gently the culture dish surface that has been coated with bacterium liquid, 4 Oxford cups of the symmetrical placement of each culture dish cross, pipette respectively the sample of 200 microlitre different quality concentration in the cup of Oxford with aseptic liquid-transfering gun, culture dish is slowly moved into constant incubator, cultivate 18h for 37 ℃, take out each culture dish colony growth situation of observing, and measure antibacterial circle diameter with slide gauge, record data, concentration for sample is respectively 5mg/ml, 10mg/ml, 15mg/ml, 20mg/ml.
2, result
Measure by above method, result is as shown in table 1.
Table 1
。
3, conclusion
Herba Sophorae alopecuroidis total alkali all has inhibitory action to main several pathogenic bacterium of bovine mastitis and endometritis, and the rising inhibitory action along with Herba Sophorae alopecuroidis total alkali concentration also progressively strengthens in certain scope, wherein the strongest to the staphylococcic inhibitory action of epidermis, the most weak to relative other the three kinds of bacterium of the inhibitory action of streptococcus agalactiae.Can further study Herba Sophorae alopecuroidis total alkali to the inhibitory action of other antibacterial, for the exploitation of Herba Sophorae alopecuroidis total alkali provide reference frame.
(2) Herba Sophorae alopecuroidis total alkali is studied acute toxicity test in mice:
Adopt the improvement karber's method to measure Herba Sophorae alopecuroidis total alkali to the acute toxicity of mouse peritoneal injection, estimate its safety, for data for clinical drug use provides foundation.During test, give the Herba Sophorae alopecuroidis total alkali of test group mouse gavaging variable concentrations, activity and the death condition of mice after the observation administration adopt improvement Kou Shi formula to calculate LD
50And LD
5095% fiducial limit, result shows, Herba Sophorae alopecuroidis total alkali is through the LD of lumbar injection
50Be 559.24mg/kg, LD
50The 95% credible 471.95mg/kg～663.59ma/kg that is limited to.Result of the test is tentatively pointed out, and the toxicity of Herba Sophorae alopecuroidis total alkali is lower, and data for clinical drug use is reliable.
1, materials and methods
1.1 material
1.1.1 experimental animal
Kunming system cleaning level healthy mice, male and female half and half, body weight (20 ± 2) g is available from Lanzhou University's experimental animal center (sweet doctor moves word 14-005).
1.1.2 tested medicine
Herba Sophorae alopecuroidis total alkali extracts preparation by Hinapharm Pharmaceutical Co., Ltd..
1.2 method
1.2.1 the extraction of Herba Sophorae alopecuroidis total alkali
Get the Herba Sophorae alopecuroidis seed and pulverize, add ethanol water to carry out ultrasonic extraction, reclaim ethanol after filtering, add 2%HCl to transfer PH, add ether washing 2 times, obtain the filtrate precipitation.Add chloroform extraction, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali again.
1.2.2 trial test
Before trial test, mice is carried out the nursing observation of 7d, in the observation period, the mice free choice feeding, drinking-water is eliminated the natural death mice.Choose 25 of healthy mices in the trial test phase, male and female half and half are divided into 5 groups at random.Every group 5, before test, fasting is 8 hours, and Herba Sophorae alopecuroidis total alkali is mixed with 5 concentration, and each organizes the isoconcentration intraperitoneal injection, determines the dosage range of formal test.
1.2.3 formal test
According to trial test, determine Dn and Dm, establish 6 dosage groups between Dn and Dm, every group of 10 mices, male and female half and half.Before test, fasting is 8 hours, freely drinks water.Observe immediately and record behavior state and the death condition of mice after intraperitoneal injection, the observation period is 7 days, calculates median lethal dose(LD 50) (LD according to experimental result
50), dead mice is in time dissected, record the pathological changes situation, observe mouse peritoneal and parenchymatous organ's pathological change.
1.2.4 LD
50Computational methods
Adopt the improvement karber's method to calculate LD
50And LD
5095% fiducial limit, computing formula is as follows:
LD
5095% fiducial limit=lg
-1(lgLD
50± 1.96 * Sx
50)
In formula: i is the group distance, and namely the log10 dose of adjacent 2 groups of dosage is poor; Xm is maximum mortality rate group dosage logarithm; Σ p is each dosage group mortality rate sum; P is each dosage group mortality rate; Q is each dosage group survival rate; N is each treated animal number; Sx
50Be lgLD
50Standard error.
2, results and analysis
2.1 trial test result
Herba Sophorae alopecuroidis total alkali is as shown in table 2 to chmice acute toxicity trial test result.Drawn by the trial test result, Dn and Dm are respectively 200mg/kg and 1000mg/kg.Determine that according to the trial test result formal test is divided into 6 dosage groups, obtains dose ratio by following formula:
In formula, r is the ratio of two adjacent groups dosage; N is required group of number of formal test.Calculate the r=0.707 of formal test.
Table 2
According to the trial test result, be made as 200mg/kg, 276.24mg/kg, 381.55mg/kg, 527.00mg/kg, 727.90mg/kg, 6 dosage groups of 1000mg/kg body weight, carry out acute toxicity test.Result is as shown in table 3.According to the data in table 3, calculated the median lethal dose(LD 50) LD of Herba Sophorae alopecuroidis total alkali to mice by formula
50=559.24mg/kg, LD
50The 95% credible 471.95mg/kg～663.59ma/kg that is limited to.Therefore judge Herba Sophorae alopecuroidis total alkali to mice through lumbar injection LD
50Greater than 500mg/kg, according to China's animal toxicology acute toxicity staging, Herba Sophorae alopecuroidis total alkali belongs to low toxicity material.
Table 3
2.2, Herba Sophorae alopecuroidis total alkali is to the acute toxicity test in mice clinical observation:
Mice disposable celiac drug administration by injection, when dosage was 200mg/kg, mice was without the phenomena of mortality within experimental period, and not dead mice symptom alleviated gradually in 2 hours, and after 12 hours, basic the recovery normally can normally be taken food and drink water; When dosage was 276.24mg/kg, dead mouse was one in 1 hour; When dosage was 381.55mg/kg, dead mouse was 2 within 15 minutes; When dosage was 527.00mg/kg, dead mouse was 3 in 10 minutes; When dosage was 727.90mg/kg, dead mouse was 6 in 10 minutes; When dosage was 1000mg/kg, mice was all dead in 5 minutes.
The main poisoning symptom of mice after intraperitoneal injection shows as lethargy, hogback, and whole body is twitched, and fore paw is scratched mouth, and has discontinuity excited.On one's deathbed present jump action before, extremity are the swimming shape then more, and are dead subsequently.
2.3, the dead mice of acute toxicity test cuts open the inspection result
Dead mice is dissected found in the abdominal cavity, a small amount of hydrops is arranged, the heart color and luster is dim, and liver has slight enlargement, and kidney is observed without significant change, and other organs is without obviously observing pathological changes.Survival mice and blank group mice were put to death and anatomic observation in 6 days afterwards, do not find seroperitoneum, other organs is without obviously observing pathological changes.
3, result
Result of the test shows, the median lethal dose(LD 50) LD of Herba Sophorae alopecuroidis total alkali to the mouse peritoneal injection
50Be 559.24mg/kg, LD
5095% the credible 471.95mg/kg～663.59ma/kg that is limited to, meet the low toxicity material grade scale.
After disposable mouse peritoneal drug administration by injection, survival mice is basic after 12 hours recovers normal, after 6 days, survival mice is carried out Anatomical Observation, and each internal organs are showed no obviously observes pathological changes.After test is described, survival mice recovers very fast, and medicine is less to its long lasting effect.
The research of Herba Sophorae alopecuroidis total alkali on mankind's clinical medicine is more with application, but the research aspect animal husbandry production is less with application, this test is estimated by the acute toxicity to Herba Sophorae alopecuroidis total alkali, for the further developmental research of Herba Sophorae alopecuroidis total alkali in animal husbandry later on should be used as a preparation.
(3) Herba Sophorae alopecuroidis total alkali infusion liquid treatment bovine mastitis clinical trial:
Herba Sophorae alopecuroidis total alkali infusion liquid treatment bovine mastitis and endometritis, effect is clear and definite, is difficult for bounce-back after curing.
From 2011 to 2012, the Herba Sophorae alopecuroidis total alkali infusion liquid is treated the bovine mastitis clinical trial.Successively select through consultation 18 milch cows that suffer from mastitis of cattle farm, Lanzhou Duan Jia stand as subjects.These 14 ill milch cow clinical symptoms main manifestations are: milk production of cow descends, and the swelling of breast part is rubescent, and heat and pain are arranged, and thin slice, floccule, pus piece or blood are arranged in milk, and milk is rare water sample or squeezes not get off.4 are ill serious, and the unexpected swelling of its breast performance, heating, hardening, breast district are responsive, show even that fervescence, diet subtract greatly or useless absolutely, down in spirits etc., look into according to clinical symptoms, Breast palpation and newborn quality inspection and be defined as clinical mastitis.
1. test and use medicine
The Herba Sophorae alopecuroidis total alkali infusion liquid: Guang Donghai Na Chuan pharmaceutcal corporation, Ltd provides, and lot number is 20110218,100ml/ bottle.
Ampicillin Sodium For Injection is available from local veterinary drug shop, and pharmaceutcal corporation, Ltd produces by expedition, and lot number is 20120308, and specification is that 5g/ props up.
2. test
2.1 test grouping
5 to 19 June in 2011, in this cattle farm 5 ill milch cows that clinical symptoms occurred as first group; There were 22 ill milch cows that clinical symptoms occurs on June 25 in same pasture to July 25, select 18 to the substantially similar ill milch cow of first group of situation as second group; Administration Herba Sophorae alopecuroidis total alkali infusion liquid, injection ampicillin are treated respectively to two groups respectively.
2.2 test method
Test group adopts the Herba Sophorae alopecuroidis total alkali infusion liquid to treat, every cattle potion, and breast perfusion, every day 2 times, each 50ml, being used in conjunction 5d is a course for the treatment of; Matched group adopts Ampicillin Sodium For Injection, breast perfusion, and the ill each 5g in newborn chamber, once a day, being used in conjunction 5d is a course for the treatment of.
3 results
Cure after medication and show as red, swollen, hot, bitterly and hard transference cure, the perusal milk sample is normal, the milk yield recovery.Cure rate with Herba Sophorae alopecuroidis total alkali infusion liquid treatment mammitis of cow is 88.8%, and the cure rate of ampicillin treatment mammitis of cow is 77.7%.
18 cow heads of Herba Sophorae alopecuroidis total alkali infusion liquid group have 16 mastitis symptoms to disappear after medication 5d or obviously change, and cure rate is 88.8%(16/18).9 cow heads of ampicillin group have 7 mastitis symptoms to disappear after medication or obviously change after five days, cure rate is 77.7%(7/9).The therapeutic effect of Herba Sophorae alopecuroidis total alkali infusion liquid group is higher than the ampicillin group.
Description of drawings
Fig. 1 is that Herba Sophorae alopecuroidis total alkali concentration is when being 5mg/ml and 20mg/ml, to colibacillary antibacterial figure.
Fig. 2 is that Herba Sophorae alopecuroidis total alkali concentration is when being 5mg/ml and 20mg/ml, to the antibacterial figure of staphylococcus aureus.
Fig. 3 is that Herba Sophorae alopecuroidis total alkali concentration is when being 5mg/ml and 20mg/ml, to the staphylococcic antibacterial figure of epidermis.
Fig. 4 is that Herba Sophorae alopecuroidis total alkali concentration is when being 5mg/ml and 20mg/ml, to the antibacterial figure of streptococcus agalactiae.
The specific embodiment
Embodiment 1:
Contain the preparation method of the Chinese medicinal perfusion liquid of Herba Sophorae alopecuroidis total alkali, comprise the following steps:
1) get the Herba Sophorae alopecuroidis seed and pulverize, add ethanol water to carry out ultrasonic extraction, reclaim ethanol after filtering, add 2% hydrochloric acid to transfer PH, add ether washing 2 times, obtain the filtrate precipitation, then add the chloroform extraction precipitation, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection to be settled to 100ml in the Herba Sophorae alopecuroidis total alkali 3g that step 1) obtains, adopt flowing steam sterilization 30min, carry out fill and get finished product Chinese medicinal perfusion liquid under aseptic condition.
Embodiment 2:
Contain the preparation method of the Chinese medicinal perfusion liquid of Herba Sophorae alopecuroidis total alkali, comprise the following steps:
1) get the Herba Sophorae alopecuroidis seed and pulverize, add ethanol water to carry out ultrasonic extraction, reclaim ethanol after filtering, add 2% hydrochloric acid to transfer PH, add ether washing 2 times, obtain the filtrate precipitation, then add the chloroform extraction precipitation, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection to be settled to 100ml in the Herba Sophorae alopecuroidis total alkali 8g that step 1) obtains, adopt flowing steam sterilization 40min, carry out fill and get finished product Chinese medicinal perfusion liquid under aseptic condition.
Embodiment 3:
Contain the preparation method of the Chinese medicinal perfusion liquid of Herba Sophorae alopecuroidis total alkali, comprise the following steps:
1) get the Herba Sophorae alopecuroidis seed and pulverize, add ethanol water to carry out ultrasonic extraction, reclaim ethanol after filtering, add 2% hydrochloric acid to transfer PH, add ether washing 2 times, obtain the filtrate precipitation, then add the chloroform extraction precipitation, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection to be settled to 100ml in the Herba Sophorae alopecuroidis total alkali 6g that step 1) obtains, adopt flowing steam sterilization 35min, carry out fill and get finished product Chinese medicinal perfusion liquid under aseptic condition.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment, the present invention is had been described in detail, for a person skilled in the art, it still can be modified to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. a Chinese medicinal perfusion liquid, is characterized in that, contains Herba Sophorae alopecuroidis total alkali in described Chinese medicinal perfusion liquid.
2. Chinese medicinal perfusion liquid according to claim 1, is characterized in that, contains Herba Sophorae alopecuroidis total alkali 3-8g in described Chinese medicinal perfusion liquid.
3. Chinese medicinal perfusion liquid according to claim 2, is characterized in that, contains Herba Sophorae alopecuroidis total alkali 6g in described Chinese medicinal perfusion liquid.
4. the preparation method of a Chinese medicinal perfusion liquid, is characterized in that, comprises the following steps:
1) get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W reclaims ethanol after filtering, and adding 2% hydrochloric acid to transfer pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, then add three precipitations of chloroform extraction, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add the water for injection standardize solution in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, carry out fill and get finished product Chinese medicinal perfusion liquid under aseptic condition.
5. the preparation method of a kind of Chinese medicinal perfusion liquid according to claim 4, is characterized in that, described step 2) in, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is 6:94.
6. Herba Sophorae alopecuroidis total alkali is for the preparation of the application in the Chinese medicinal perfusion liquid for the treatment of bovine mastitis and endometritis.
7. application according to claim 6, is characterized in that, the use amount of described Chinese medicinal perfusion liquid is each breast district 30-60ml of every cow head.
Priority Applications (1)
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| CN103271983A (en) * | 2013-05-17 | 2013-09-04 | 广东海纳川药业股份有限公司 | Perfusate for treating endometritis and kysthitis of pig as well as preparation method and application thereof |
| CN104208136A (en) * | 2014-09-25 | 2014-12-17 | 宁夏智弘生物科技有限公司 | Preparation process and using method of sophora alopecuroide alkaloid dairy cow breast injectant |
| CN112206250A (en) * | 2020-10-26 | 2021-01-12 | 宁夏职业技术学院（宁夏广播电视大学） | Application of sophora alopecuroide extract in preparation of drugs or bacteriostatic agents for treating drug-resistant diseases of poultry |
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| RU2700081C1 (en) * | 2019-01-14 | 2019-09-12 | Общество с ограниченной ответственностью "Агровейт" | Preparation "berkana" for prevention and treatment of endometritis in cows and a method for production thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103271983A (en) * | 2013-05-17 | 2013-09-04 | 广东海纳川药业股份有限公司 | Perfusate for treating endometritis and kysthitis of pig as well as preparation method and application thereof |
| CN103271983B (en) * | 2013-05-17 | 2016-05-18 | 广东海纳川生物科技股份有限公司 | A kind of endometritis and colpitic perfusion liquid, its preparation method and application for the treatment of pig |
| CN104208136A (en) * | 2014-09-25 | 2014-12-17 | 宁夏智弘生物科技有限公司 | Preparation process and using method of sophora alopecuroide alkaloid dairy cow breast injectant |
| CN112206250A (en) * | 2020-10-26 | 2021-01-12 | 宁夏职业技术学院（宁夏广播电视大学） | Application of sophora alopecuroide extract in preparation of drugs or bacteriostatic agents for treating drug-resistant diseases of poultry |
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| CN103142683B (en) | 2015-09-09 |
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