CN103694371A - Preparation method of honey raisin tree polysaccharide - Google Patents
Preparation method of honey raisin tree polysaccharide Download PDFInfo
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- CN103694371A CN103694371A CN201410011625.8A CN201410011625A CN103694371A CN 103694371 A CN103694371 A CN 103694371A CN 201410011625 A CN201410011625 A CN 201410011625A CN 103694371 A CN103694371 A CN 103694371A
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- polysaccharide
- raisin tree
- honey raisin
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Abstract
The invention discloses a preparation method of honey raisin tree polysaccharide. The method comprises the following steps: (1) drying, crushing and degreasing honey raisin tree pulp; (2) extracting honey raisin tree polysaccharide in a vacuum pulsing manner by adopting a complex enzyme preparation, extracting twice, merging extracting solutions, and concentrating in vacuum for later use; (3) carrying out alcohol precipitation on the concentrated honey raisin tree polysaccharide extracting solution, centrifugally separating and drying in vacuum, so as to obtain a honey raisin tree polysaccharide product. The honey raisin tree polysaccharide is extracted by adopting a complex enzyme method, so that the method has the characteristics of short extraction time, high polysaccharide yield, and good storage of active ingredients. Mass transfer is reinforced by vacuum pulsing in the polysaccharide extracting process, so that the efficiency is further improved, and the extraction time is shortened.
Description
Technical field
The present invention relates to a kind of preparation method of hovenia polysaccharides, specifically refer to adopt compound enzymic preparation enzymolysis and vacuum pulsating to extract to prepare the method for hovenia polysaccharides.
Background technology
Polysaccharide is the macromolecular substance in natural organic-compound, there is health care and pharmaceutical use, as lentinan has, have the effect of antitumor and enhancing immunologic function etc., a lot of important physiological processs are all closely related with polysaccharide molecule, and these have caused domestic and international scientific worker's attention.
Honey raisin tree has clearing away heat and promoting diuresis, separates drunkly not wake up, and dispelling the wind and dampness pathogens, the effects such as falling fire removing toxic substances of quenching the thirst, these effects are most relevant with the polysaccharide in honey raisin tree, make the extraction of hovenia polysaccharides have important practical value.Forefathers adopt neutral hot water lixiviate or acid, alkali lixiviate to the extraction of hovenia polysaccharides, and the former is long the time, and yield is low, the ad hoc structure relevant with activity in the latter's destructible polysaccharide.Thereby prior art can not meet the requirement that hovenia polysaccharides extracts.
Summary of the invention
The problem existing for solving the above-mentioned prior art of extracting polysaccharide from honey raisin tree, the inventor, through a large amount of scientific practices, invents a kind of extraction conditions gentle finally, and the time is short, and extraction yield is high, the hovenia polysaccharides preparation method that activeconstituents is kept.
The present invention includes following step:
(1) processing of honey raisin tree: honey raisin tree pulp is dry, also degreasing of pulverizing.
(2) extraction of hovenia polysaccharides: material is placed in after extractor, adds quality of material 5-15 water doubly, the papoid of the cellulase of quality of material 0.8-1.2%, the polygalacturonase of 1.3%-1.7%, 0.8-1.2%, the enzyme work of its cellulase is 20 * 10
4umg
-1-40 * 10
4umg
-1, the enzyme work of polygalacturonase is 1 * 10
4umg
-1-3 * 10
4umg
-1, the enzyme work of papoid is 80 * 10
4umg
-1-120 * 10
4umg
-1, adjusting pH value is 3.5-4.5, temperature is 40-50 ℃, extracts 30-90 minute, filters also repetitive operation and once, merges the filtrate obtaining for twice, standby after vacuum concentration.
In said extracted process, extractor is carried out to step vacuumizing and vacuum breaker, utilize vacuum pulsation reinforcing mass transfer.The mechanism of vacuum pulsation reinforcing mass transfer is: when vacuumizing, honey raisin tree ICP power is greater than honey raisin tree histocyte external pressure, and the solvent that has dissolved polysaccharide can be forced through honey raisin tree cell tissue under differential pressure action; When vacuum breaker, honey raisin tree histocyte external pressure is greater than in honey raisin tree histocyte and presses, solvent outside honey raisin tree histocyte enters rapidly honey raisin tree histocyte inside and removes to dissolve hovenia polysaccharides because of differential pressure action, alternately changing and strengthened solvent and be dissolved in the polysaccharide mass transfer in solvent of this honey raisin tree histocyte external and internal pressure, reduces extraction efficiency raising, residual rate.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, and the concentration that makes ethanol is 50-80%, now hovenia polysaccharides can parse, after centrifugation vacuum-drying, obtain hovenia polysaccharides product, in product, the massfraction of polysaccharide is 50%-60%.
Hovenia polysaccharides is widely used in the fields such as food, medicine and chemical industry, has a extensive future.
In vegetable polysaccharides extracts, conventional extraction enzyme has proteolytic enzyme, cellulase and polygalacturonase.
Proteolytic enzyme has hydrolytic action to plant protein, and it adds can make plant structure become loose; Simultaneously proteolytic enzyme also can make glycoprotein and the hydrolysis of glycan Free Proteins, has reduced the bonding force of they and raw material, is conducive to the leaching of vegetable polysaccharides.Because proteolytic enzyme has the effect except albumen, in the extracting solution of its participation, only contain a small amount of protein, can alleviate the task of next step isolating protein, so proteolytic enzyme involved in plant polysaccharide extraction method is a kind of desirable extracting method.
Cellulase is a kind of prozyme, belongs to biological catalyst, and in plant, the general content of fiber is higher, and target product polysaccharide is wrapped, depends among fiber, is difficult to discharge from cell walls.Cellulase can destroy the cell walls that hinders polysaccharide stripping, shortens extraction time and cycle, and starch and cellulosic are converted into carbohydrate, promotes the carrying out of whole leaching process.Cellulase participates in, after reaction, within very short time, just can will depending on the cellulose decomposition of warding off more, and whole leaching process can complete at short notice.
In leaching process, there is peptization in the starch in some plant, pectin, phlegmatic temperament etc., make filtration difficulty, during evaporation concentration operational cost in hot water.Polygalacturonase can decompose cell walls middle layer and insoluble polymer protopectin-, reduces viscosity, and can make polysaccharide fully dissolve, and improves the utilization ratio of raw material.
Compared with prior art, the invention has the beneficial effects as follows: the compound enzymic preparation that adopts cellulase, polygalacturonase and papoid, utilize the synergy of plurality of enzymes, bring into play to greatest extent the effect of Enzymatic Extraction, and the prozyme system of introducing is to extract hovenia polysaccharides under 40-50 ℃ of cold condition, suppressed the reduction of hovenia polysaccharides activity under high temperature extraction condition, make hovenia polysaccharides activity under impregnable prerequisite, in short period, allow polysaccharide discharge from honey raisin tree cell, also improved extraction yield.
The present invention adopts and extracts hovenia polysaccharides with combined-enzyme method, has the advantages that the time is short, polysaccharide yield is high, activeconstituents is kept, and compares with conventional extraction method, and extraction time shortens 30-60%, and polysaccharide yield improves 10-25%, and activity also has obvious lifting.
The present invention also, in polysaccharide leaching process, utilizes vacuum pulsation reinforcing mass transfer, has further improved efficiency, has shortened extraction time.
Embodiment
Below the preparation method of hovenia polysaccharides is elaborated.
Embodiment 1:
(1) processing of honey raisin tree: honey raisin tree pulp is dry, also degreasing of pulverizing.
(2) extraction of hovenia polysaccharides: material is placed in after extractor, adds the water of 10 times of quality of material, the cellulase of quality of material 1.15%, 1.48% polygalacturonase, 0.98% papoid, wherein cellulosic enzyme work is 29 * 10
4umg
-1, the enzyme work of polygalacturonase is 1.2 * 10
4umg
-1, the enzyme work of papoid is 101 * 10
4umg
-1, regulating pH value is 3.6, temperature is 45 ℃, extracts 61 minutes, filters also repetitive operation and once, merges the filtrate obtaining for twice, standby after vacuum concentration.
In said extracted process, extractor is carried out to step vacuumizing and vacuum breaker, utilize vacuum pulsation reinforcing mass transfer.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, and the concentration that makes ethanol is 58%, now hovenia polysaccharides can parse, after centrifugation vacuum-drying, obtain hovenia polysaccharides product, in product, the massfraction of polysaccharide is 51%.
Embodiment 2:
(1) processing of honey raisin tree: honey raisin tree pulp is dry, also degreasing of pulverizing.
(2) extraction of hovenia polysaccharides: material is placed in after extractor, adds the water of 6 times of quality of material, the cellulase of quality of material 0.81%, 1.33% polygalacturonase, 1.15% papoid, the enzyme work of its cellulase is 21 * 10
4umg
-1, the enzyme work of polygalacturonase is 1.9 * 10
4umg
-1, the enzyme work of papoid is 82 * 10
4umg
-1, regulating pH value is 4.1, temperature is 41 ℃, extracts 32 minutes, filters also repetitive operation and once, merges the filtrate obtaining for twice, standby after vacuum concentration.
In said extracted process, extractor is carried out to step vacuumizing and vacuum breaker, utilize vacuum pulsation reinforcing mass transfer.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, and the concentration that makes ethanol is 66%, now hovenia polysaccharides can parse, after centrifugation vacuum-drying, obtain hovenia polysaccharides product, in product, the massfraction of polysaccharide is 58%.
Embodiment 3:
(1) processing of honey raisin tree: honey raisin tree pulp is dry, also degreasing of pulverizing.
(2) extraction of hovenia polysaccharides: material is placed in after extractor, adds the water of 14 times of quality of material, the cellulase of quality of material 1.02%, 1.67% polygalacturonase, 0.83% papoid, the enzyme work of its cellulase is 38 * 10
4umg
-1, the enzyme work of polygalacturonase is 2.7 * 10
4umg
-1, the enzyme work of papoid is 117 * 10
4umg
-1, regulating pH value is 4.4, temperature is 49 ℃, extracts 87 minutes, filters also repetitive operation and once, merges the filtrate obtaining for twice, standby after vacuum concentration.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, and the concentration that makes ethanol is 78%, now hovenia polysaccharides can parse, after centrifugation vacuum-drying, obtain hovenia polysaccharides product, in product, the massfraction of polysaccharide is 55%.
Claims (3)
1. a preparation method for hovenia polysaccharides, is characterized in that comprising following step:
(1) processing of honey raisin tree: honey raisin tree pulp is dry, also degreasing of pulverizing;
(2) extraction of hovenia polysaccharides: material is placed in after extractor, adds quality of material 5-15 water doubly, the papoid of the cellulase of quality of material 0.8-1.2%, the polygalacturonase of 1.3%-1.7%, 0.8-1.2%, the enzyme work of its cellulase is 20 * 10
4umg
-1-40 * 10
4umg
-1, the enzyme work of polygalacturonase is 1 * 10
4umg
-1-3 * 10
4umg
-1, the enzyme work of papoid is 80 * 10
4umg
-1-120 * 10
4umg
-1, adjusting pH value is 3.5-4.5, temperature is 40-50 ℃, extracts 30-90 minute, filters also repetitive operation and once, merges the filtrate obtaining for twice, standby after vacuum concentration;
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, the concentration that makes ethanol is 50-80%, obtains hovenia polysaccharides product after centrifugation vacuum-drying.
2. the preparation method of hovenia polysaccharides according to claim 1, is characterized in that: in hovenia polysaccharides leaching process, extractor is carried out to step vacuumizing and vacuum breaker, utilize vacuum pulsation reinforcing mass transfer.
3. the hovenia polysaccharides product that method obtains according to claim 1, is characterized in that: in product, the massfraction of polysaccharide is 50%-60%.
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| CN201410011625.8A CN103694371B (en) | 2014-01-10 | 2014-01-10 | A kind of preparation method of hovenia polysaccharides |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136362A (en) * | 2017-06-13 | 2017-09-08 | 旬阳县太极缘生物科技有限公司 | A kind of honey raisin tree deep process and its honey raisin tree meat, honey raisin tree juice and honey raisin tree slag |
| CN107858388A (en) * | 2017-10-17 | 2018-03-30 | 陕西科技大学 | A kind of method that sheep breast Antihypertensive Peptides are prepared using probiotic lactobacilli protease |
| CN110652005A (en) * | 2019-09-25 | 2020-01-07 | 上海诺德生物实业有限公司 | Method for preparing hovenia dulcis thunb extract by enzymolysis method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002060463A1 (en) * | 2001-01-31 | 2002-08-08 | Lifetree Biotech Co., Ltd. | Lower alcohol-insoluble extract of hovenia dulcis var. koreana nakai, a polysaccharide isolated therefrom and an antihepatotoxic composition containing same |
| CN102002108A (en) * | 2010-11-25 | 2011-04-06 | 南京泽朗医药科技有限公司 | Method for preparing hovenia polysaccharides |
| CN102477104A (en) * | 2010-11-25 | 2012-05-30 | 苏州宝泽堂医药科技有限公司 | Method for separating and purifying polysaccharide from Hovenia acerba |
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2014
- 2014-01-10 CN CN201410011625.8A patent/CN103694371B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002060463A1 (en) * | 2001-01-31 | 2002-08-08 | Lifetree Biotech Co., Ltd. | Lower alcohol-insoluble extract of hovenia dulcis var. koreana nakai, a polysaccharide isolated therefrom and an antihepatotoxic composition containing same |
| CN102002108A (en) * | 2010-11-25 | 2011-04-06 | 南京泽朗医药科技有限公司 | Method for preparing hovenia polysaccharides |
| CN102477104A (en) * | 2010-11-25 | 2012-05-30 | 苏州宝泽堂医药科技有限公司 | Method for separating and purifying polysaccharide from Hovenia acerba |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136362A (en) * | 2017-06-13 | 2017-09-08 | 旬阳县太极缘生物科技有限公司 | A kind of honey raisin tree deep process and its honey raisin tree meat, honey raisin tree juice and honey raisin tree slag |
| CN107858388A (en) * | 2017-10-17 | 2018-03-30 | 陕西科技大学 | A kind of method that sheep breast Antihypertensive Peptides are prepared using probiotic lactobacilli protease |
| CN110652005A (en) * | 2019-09-25 | 2020-01-07 | 上海诺德生物实业有限公司 | Method for preparing hovenia dulcis thunb extract by enzymolysis method |
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| CN103694371B (en) | 2015-08-19 |
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Inventor after: Yang Wangen Inventor after: Huang Qun Inventor after: Ma Chengjin Inventor after: Wang Jue Inventor after: Jie Yajiao Inventor before: Huang Qun Inventor before: Yang Wangen Inventor before: Ma Chengjin Inventor before: Wang Jue Inventor before: Jie Yajiao |
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Free format text: CORRECT: INVENTOR; FROM: HUANG QUN YANG WANGEN MA CHENGJIN WANG JUE JIE YAJIAO TO: YANG WANGEN HUANG QUN MA CHENGJIN WANG JUE JIE YAJIAO |
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