CN102040665A - Method for extracting sea cucumber polysaccharide - Google Patents
Method for extracting sea cucumber polysaccharide Download PDFInfo
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- CN102040665A CN102040665A CN2009101975629A CN200910197562A CN102040665A CN 102040665 A CN102040665 A CN 102040665A CN 2009101975629 A CN2009101975629 A CN 2009101975629A CN 200910197562 A CN200910197562 A CN 200910197562A CN 102040665 A CN102040665 A CN 102040665A
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- Prior art keywords
- sea cucumber
- extracting method
- described step
- cucumber polysaccharide
- polysaccharide according
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- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to a method for extracting sea cucumber polysaccharides, which comprises the steps of: heating and carrying out homogenate on dry sea cucumbers processed from fresh sea cucumbers, carrying out enzymolysis, adjusting the pH value, purifying by using ethanol with high concentration, filtrating and separating, centrifuging sediments, and maintaining the largest active substances of the processed sea cucumber polysaccharides after being frozen and dried. The extraction and purification process is relatively simple, and the products can meet requirements and can be further processed to be used as materials of injections.
Description
Technical field
The present invention relates to the extracting method of sea cucumber polysaccharide, kept the purity of the nutrient substance of sea cucumber polysaccharide to greatest extent
Background technology
Sea cucumber polysaccharide has multiple physiologically active; physiologically active mainly shows as: (1) antitumor action (2) improves immunizing power (3) anticoagulation (4) and promotes celloglobulin dissolving (5) to suppress embolisms (6) hypoglycemic (7) to have simple scar exanthema virus HSV effect (10) Stichopus japonicus mucopolysaccharide of virus of AIDS HIV (8) hepatitis B viruses (HBV) of inhibition (9) and can prevent osteoporosis, can be used as the anti-radioactivity of treatment of arthritis (11), radiation (12) delay senility (13) cortical neuron wound or the apoptosis that causes because of μ-starch is protein induced had provide protection etc.Sea cucumber is as a kind of marine drug, and nutritive ingredient is very abundant, and activeconstituents is also varied.The existing method of extracting sea cucumber polysaccharide, a large amount of many isoreactivities of sea cucumber material work in-processes runs off, and extracting method of the present invention guarantees the purity and the activity of sea cucumber polysaccharide.
Summary of the invention
At the defective of prior art, the invention discloses a kind of method that as far as possible keeps its sea cucumber polysaccharide active ingredient.
The present invention compared with prior art has the following advantages owing to adopt above technical scheme:
Utilize boiled sea cucumber juice to carry out elder generation and transfer pH value, carry out homogenate, transfer pH value again, boil back centrifugation, add ethanol sedimentation, the throw out that will sink filters postlyophilization.
Embodiment
The present invention further specifies the present invention with following specific embodiments.(1) immersion of dry product sea cucumber is swollen;
(2) the naughty internal organ that go;
(3) smash homogenate to pieces;
(4) transfer the ph value;
(5) homogenate;
(6) add protease hydrolysis;
(7) transfer the ph value;
(8) add trypsin hydrolyzing;
(9) boil;
(10) centrifugal sediment;
(11) transfer the ph value;
(12) add ethanol sedimentation;
(13) centrifugal sediment;
(14) throw out filters postlyophilization;
(15) finished product packing.
Claims (10)
1. the extracting method of sea cucumber polysaccharide is characterized in that, comprises following steps:
(1) immersion of dry product sea cucumber is swollen;
(2) the naughty internal organ that go;
(3) smash homogenate to pieces;
(4) transfer the ph value;
(5) homogenate;
(6) add protease hydrolysis;
(7) transfer the ph value;
(8) add trypsin hydrolyzing;
(9) boil;
(10) centrifugal sediment;
(11) transfer the ph value;
(12) add ethanol sedimentation;
(13) centrifugal sediment;
(14) throw out filters postlyophilization;
(15) finished product packing.
2. the extracting method of sea cucumber polysaccharide according to claim 1 is characterized in that, in the described step () sea cucumber in the ratio of water less than 1: 4.
3. the extracting method of sea cucumber polysaccharide according to claim 2 is characterized in that, the temperature of smashing homogenate in the described step (three) to pieces is less than 50 ℃.
4. the extracting method of sea cucumber polysaccharide according to claim 3 is characterized in that, ph value regulation range is 1-3 in the described step (four).
5. the extracting method of sea cucumber polysaccharide according to claim 4 is characterized in that, adding proteolytic enzyme in the described step (six) is acid resistance proteolytic enzyme.
6. the extracting method of sea cucumber polysaccharide according to claim 5 is characterized in that, ph value regulation range is 8-10 in the described step (four).
7. the extracting method of sea cucumber polysaccharide according to claim 6 is characterized in that, adding proteolytic enzyme in the described step (six) is acid resistance proteolytic enzyme.
8. the extracting method of sea cucumber polysaccharide according to claim 7 is characterized in that, adding proteolytic enzyme in the described step (seven) is alkali resistance proteolytic enzyme.
9. the extracting method of sea cucumber polysaccharide according to claim 8 is characterized in that, the ph value is adjusted to 7 in the described step (11).
10. the extracting method of sea cucumber polysaccharide according to claim 9 is characterized in that, adds 95% ethanol in the described step (12) and makes solution contain amount of alcohol to reach 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101975629A CN102040665A (en) | 2009-10-22 | 2009-10-22 | Method for extracting sea cucumber polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101975629A CN102040665A (en) | 2009-10-22 | 2009-10-22 | Method for extracting sea cucumber polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102040665A true CN102040665A (en) | 2011-05-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101975629A Pending CN102040665A (en) | 2009-10-22 | 2009-10-22 | Method for extracting sea cucumber polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102040665A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102960605A (en) * | 2012-12-17 | 2013-03-13 | 江苏海辰科技集团有限公司 | Preparation method of sea cucumber mucopolysaccharide |
| CN103724442A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Preparation method for low-protein and high-purity sea cucumber polysaccharide |
| CN103951759A (en) * | 2014-04-22 | 2014-07-30 | 钦州市山海生物科技有限公司 | Method for extracting sea cucumber polysaccharide from sea cucumber |
| CN115067484A (en) * | 2022-06-27 | 2022-09-20 | 泸州品创科技有限公司 | Sea cucumber base material and preparation method and application thereof |
-
2009
- 2009-10-22 CN CN2009101975629A patent/CN102040665A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406048A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102406048B (en) * | 2011-11-29 | 2013-04-17 | 山东好当家海洋发展股份有限公司 | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution |
| CN102960605A (en) * | 2012-12-17 | 2013-03-13 | 江苏海辰科技集团有限公司 | Preparation method of sea cucumber mucopolysaccharide |
| CN103724442A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Preparation method for low-protein and high-purity sea cucumber polysaccharide |
| CN103951759A (en) * | 2014-04-22 | 2014-07-30 | 钦州市山海生物科技有限公司 | Method for extracting sea cucumber polysaccharide from sea cucumber |
| CN115067484A (en) * | 2022-06-27 | 2022-09-20 | 泸州品创科技有限公司 | Sea cucumber base material and preparation method and application thereof |
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| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20110504 |