CN103694371B - A kind of preparation method of hovenia polysaccharides - Google Patents
A kind of preparation method of hovenia polysaccharides Download PDFInfo
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- CN103694371B CN103694371B CN201410011625.8A CN201410011625A CN103694371B CN 103694371 B CN103694371 B CN 103694371B CN 201410011625 A CN201410011625 A CN 201410011625A CN 103694371 B CN103694371 B CN 103694371B
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- hovenia
- polysaccharides
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Abstract
The invention discloses a kind of preparation method of hovenia polysaccharides, honey raisin tree pulp is dry, pulverizing also degreasing that the method comprises (1); (2) adopt compound enzymic preparation vacuum impulse formula to extract hovenia polysaccharides, extract twice, united extraction liquid, for subsequent use after vacuum concentration; (3) the hovenia polysaccharides extracting solution after concentrated is carried out alcohol precipitation, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying.The present invention adopts combined-enzyme method to extract hovenia polysaccharides, have the advantages that extraction time is short, polysaccharide yield is high, activeconstituents is kept, the present invention, also in Polyose extraction process, utilizes vacuum impulse reinforcing mass transfer, further increases efficiency, shortens extraction time.
Description
Technical field
The present invention relates to a kind of preparation method of hovenia polysaccharides, specifically refer to adopt compound enzymic preparation enzymolysis and vacuum impulse formula to extract the method preparing hovenia polysaccharides.
Background technology
Polysaccharide is the macromolecular substance in natural organic-compound, there is health care and pharmaceutical use, have effect etc. that is antitumor and enhancing immunologic function as lentinan has, much important physiological process is all closely related with polysaccharide molecule, and these have caused the attention of domestic and international scientific worker.
Honey raisin tree has clearing away heat and promoting diuresis, separates drunkly not wake up, dispelling the wind and dampness pathogens, and effects such as falling fire removing toxic substances of quenching the thirst, these effects are most relevant with the polysaccharide in honey raisin tree, make the extraction of hovenia polysaccharides have important practical value.The extraction of forefathers to hovenia polysaccharides adopts neutral hot water extraction or acid, alkali lixiviate more, and the former is long for the time, and yield is low, ad hoc structure relevant with activity in the latter's destructible polysaccharide.Thus, prior art can not meet the requirement that hovenia polysaccharides extracts.
Summary of the invention
For solving the above-mentioned prior art Problems existing extracting polysaccharide from honey raisin tree, the present inventor is through a large amount of scientific practices, and finally invent a kind of extraction conditions gentle, the time is short, and extraction yield is high, the hovenia polysaccharides preparation method that activeconstituents is kept.
The present invention includes following step:
(1) process of honey raisin tree: honey raisin tree pulp is dry, pulverizing also degreasing.
(2) extraction of hovenia polysaccharides: after material is placed in extractor, add quality of material 5-15 water doubly, the papoid of the cellulase of quality of material 0.8-1.2%, the polygalacturonase of 1.3%-1.7%, 0.8-1.2%, the enzyme work of its cellulase is 20 × 10
4umg
-1-40 × 10
4umg
-1, the enzyme work of polygalacturonase is 1 × 10
4umg
-1-3 × 10
4umg
-1, the enzyme work of papoid is 80 × 10
4umg
-1-120 × 10
4umg
-1, adjustment pH value is 3.5-4.5, and temperature is 40-50 DEG C, extracts 30-90 minute, filters also repetitive operation and once, merges the filtrate obtained for twice, for subsequent use after vacuum concentration.
In said extracted process, step vacuumizing and vacuum breaker is carried out to extractor, utilize vacuum impulse reinforcing mass transfer.The mechanism of vacuum impulse reinforcing mass transfer is: when evacuated, and honey raisin tree ICP power is greater than honey raisin tree histocyte external pressure, and the solvent having dissolved polysaccharide can be forced through honey raisin tree cell tissue under differential pressure action; When vacuum breaker, honey raisin tree histocyte external pressure is greater than pressure in honey raisin tree histocyte, solvent outside honey raisin tree histocyte enters rapidly honey raisin tree histocyte inside and goes to dissolve hovenia polysaccharides because of differential pressure action, the change that replaces of this honey raisin tree histocyte external and internal pressure enhances solvent and dissolving polysaccharide mass transfer in a solvent, makes extraction efficiency raising, residual rate reduction.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, make the concentration of ethanol be 50-80%, now hovenia polysaccharides can parse, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying, and in product, the massfraction of polysaccharide is 50%-60%.
Hovenia polysaccharides is widely used in the fields such as food, medicine and chemical industry, has a extensive future.
In vegetable polysaccharides extracts, conventional extraction enzyme has proteolytic enzyme, cellulase and polygalacturonase.
Proteolytic enzyme has hydrolytic action to plant protein, and it adds can make plant structure become loose; Simultaneously proteolytic enzyme also can make glycoprotein and the hydrolysis of glycan Free Proteins, reduces the bonding force of they and raw material, is conducive to the leaching of vegetable polysaccharides.Because proteolytic enzyme has the effect of removing protein, only containing a small amount of protein in its extracting solution participated in, the task of next step isolating protein can be alleviated, so proteolytic enzyme involved in plant Polyose extraction method is a kind of desirable extracting method.
Cellulase is a kind of prozyme, and belong to biological catalyst, in plant, the general content of fiber is higher, and target product polysaccharide, be wrapped, depend among fiber, be difficult to discharge from cell walls.Cellulase can destroy the cell walls hindering polysaccharide stripping, shortens extraction time and cycle, and starch and cellulosic are converted into carbohydrate, promote the carrying out of whole leaching process.After cellulase participates in reaction, within very short time, just can depend on the cellulose decomposition warded off, whole leaching process can complete at short notice more.
In leaching process, there is peptization in the starch in some plant, pectin, phlegmatic temperament etc., makes filtration difficulty, during evaporation concentration operational cost in the hot water.Polygalacturonase can decompose cell walls middle layer and insoluble polymer protopectin-, reduces viscosity, and polysaccharide can be made fully to dissolve, and improves the utilization ratio of raw material.
Compared with prior art, the invention has the beneficial effects as follows: the compound enzymic preparation adopting cellulase, polygalacturonase and papoid, utilize the synergy of multiple enzyme, play effect of Enzymatic Extraction to greatest extent, and the prozyme system introduced extracts hovenia polysaccharides under 40-50 DEG C of cold condition, inhibit the reduction that hovenia polysaccharides is active under high temperature extraction condition, make hovenia polysaccharides active under impregnable prerequisite, allow polysaccharide discharge from honey raisin tree cell in short period, also improve extraction yield.
Employing combined-enzyme method of the present invention extracts hovenia polysaccharides, has the advantages that the time is short, polysaccharide yield is high, activeconstituents is kept, and compared with traditional extraction method, extraction time shortens 30-60%, and polysaccharide yield improves 10-25%, and activity also has obvious lifting.
The present invention, also in Polyose extraction process, utilizes vacuum impulse reinforcing mass transfer, further increases efficiency, shortens extraction time.
Embodiment
Below the preparation method of hovenia polysaccharides is described in detail.
Embodiment 1:
(1) process of honey raisin tree: honey raisin tree pulp is dry, pulverizing also degreasing.
(2) extraction of hovenia polysaccharides: after material is placed in extractor, adds the water of quality of material 10 times, the cellulase of quality of material 1.15%, the polygalacturonase of 1.48%, 0.98% papoid, wherein cellulosic enzyme work is 29 × 10
4umg
-1, the enzyme work of polygalacturonase is 1.2 × 10
4umg
-1, the enzyme work of papoid is 101 × 10
4umg
-1, regulate pH value to be 3.6, temperature is 45 DEG C, extracts 61 minutes, filters also repetitive operation and once, merges the filtrate obtained for twice, for subsequent use after vacuum concentration.
In said extracted process, step vacuumizing and vacuum breaker is carried out to extractor, utilize vacuum impulse reinforcing mass transfer.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, make the concentration of ethanol be 58%, now hovenia polysaccharides can parse, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying, and in product, the massfraction of polysaccharide is 51%.
Embodiment 2:
(1) process of honey raisin tree: honey raisin tree pulp is dry, pulverizing also degreasing.
(2) extraction of hovenia polysaccharides: after material is placed in extractor, adds the water of quality of material 6 times, the cellulase of quality of material 0.81%, the polygalacturonase of 1.33%, 1.15% papoid, the enzyme work of its cellulase is 21 × 10
4umg
-1, the enzyme work of polygalacturonase is 1.9 × 10
4umg
-1, the enzyme work of papoid is 82 × 10
4umg
-1, regulate pH value to be 4.1, temperature is 41 DEG C, extracts 32 minutes, filters also repetitive operation and once, merges the filtrate obtained for twice, for subsequent use after vacuum concentration.
In said extracted process, step vacuumizing and vacuum breaker is carried out to extractor, utilize vacuum impulse reinforcing mass transfer.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, make the concentration of ethanol be 66%, now hovenia polysaccharides can parse, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying, and in product, the massfraction of polysaccharide is 58%.
Embodiment 3:
(1) process of honey raisin tree: honey raisin tree pulp is dry, pulverizing also degreasing.
(2) extraction of hovenia polysaccharides: after material is placed in extractor, adds the water of quality of material 14 times, the cellulase of quality of material 1.02%, the polygalacturonase of 1.67%, 0.83% papoid, the enzyme work of its cellulase is 38 × 10
4umg
-1, the enzyme work of polygalacturonase is 2.7 × 10
4umg
-1, the enzyme work of papoid is 117 × 10
4umg
-1, regulate pH value to be 4.4, temperature is 49 DEG C, extracts 87 minutes, filters also repetitive operation and once, merges the filtrate obtained for twice, for subsequent use after vacuum concentration.
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, make the concentration of ethanol be 78%, now hovenia polysaccharides can parse, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying, and in product, the massfraction of polysaccharide is 55%.
Claims (3)
1. a preparation method for hovenia polysaccharides, is characterized in that comprising following step:
(1) process of honey raisin tree: honey raisin tree pulp is dry, pulverizing also degreasing;
(2) extraction of hovenia polysaccharides: after material is placed in extractor, add quality of material 5-15 water doubly, the papoid of the cellulase of quality of material 0.8-1.2%, the polygalacturonase of 1.3%-1.7%, 0.8-1.2%, the enzyme work of its cellulase is 20 × 10
4umg
-1-40 × 10
4umg
-1, the enzyme work of polygalacturonase is 1 × 10
4umg
-1-3 × 10
4umg
-1, the enzyme work of papoid is 80 × 10
4umg
-1-120 × 10
4umg
-1, adjust ph is 3.5-4.5, and temperature is 40-50 DEG C, extracts 30-90 minute, filters also repetitive operation and once, merges the filtrate obtained for twice, for subsequent use after vacuum concentration;
(3) parsing of hovenia polysaccharides is with dry: by the hovenia polysaccharides extracting liquid filtering after concentrated, and add ethanol, make the concentration of ethanol be 50-80%, namely centrifugation also obtains hovenia polysaccharides product after vacuum-drying.
2. the preparation method of hovenia polysaccharides according to claim 1, is characterized in that: carry out step vacuumizing and vacuum breaker to extractor in hovenia polysaccharides leaching process, utilize vacuum impulse reinforcing mass transfer.
3. the hovenia polysaccharides product that obtains of method according to claim 1, is characterized in that: in product, the massfraction of polysaccharide is 50%-60%.
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| CN107136362A (en) * | 2017-06-13 | 2017-09-08 | 旬阳县太极缘生物科技有限公司 | A kind of honey raisin tree deep process and its honey raisin tree meat, honey raisin tree juice and honey raisin tree slag |
| CN107858388A (en) * | 2017-10-17 | 2018-03-30 | 陕西科技大学 | A kind of method that sheep breast Antihypertensive Peptides are prepared using probiotic lactobacilli protease |
| CN110652005A (en) * | 2019-09-25 | 2020-01-07 | 上海诺德生物实业有限公司 | Method for preparing hovenia dulcis thunb extract by enzymolysis method |
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| KR100403721B1 (en) * | 2001-01-31 | 2003-11-05 | (주)생명의나무 | Lower alcohol insoluble extract and a polysaccharide therein isolated from hovenia dulcis var. koreana nakai having antihepatotoxic and anti-hangover activity and composition containing same |
| CN102002108A (en) * | 2010-11-25 | 2011-04-06 | 南京泽朗医药科技有限公司 | Method for preparing hovenia polysaccharides |
| CN102477104A (en) * | 2010-11-25 | 2012-05-30 | 苏州宝泽堂医药科技有限公司 | Method for separating and purifying polysaccharide from Hovenia acerba |
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Inventor after: Yang Wangen Inventor after: Huang Qun Inventor after: Ma Chengjin Inventor after: Wang Jue Inventor after: Jie Yajiao Inventor before: Huang Qun Inventor before: Yang Wangen Inventor before: Ma Chengjin Inventor before: Wang Jue Inventor before: Jie Yajiao |
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