CN103690396B - Facial film of pbz polymer polyglutamic acid and preparation method thereof - Google Patents
Facial film of pbz polymer polyglutamic acid and preparation method thereof Download PDFInfo
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- CN103690396B CN103690396B CN201310750171.1A CN201310750171A CN103690396B CN 103690396 B CN103690396 B CN 103690396B CN 201310750171 A CN201310750171 A CN 201310750171A CN 103690396 B CN103690396 B CN 103690396B
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- 230000001815 facial effect Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 229920000642 polymer Polymers 0.000 title claims abstract description 13
- 108010020346 Polyglutamic Acid Proteins 0.000 title claims description 26
- 229920002643 polyglutamic acid Polymers 0.000 title claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 22
- 239000012153 distilled water Substances 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 13
- 102000008186 Collagen Human genes 0.000 claims abstract description 12
- 108010035532 Collagen Proteins 0.000 claims abstract description 12
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 11
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 11
- 239000011718 vitamin C Substances 0.000 claims abstract description 11
- 241001116389 Aloe Species 0.000 claims abstract description 10
- 244000293323 Cosmos caudatus Species 0.000 claims abstract description 10
- 235000005956 Cosmos caudatus Nutrition 0.000 claims abstract description 10
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 10
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 10
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 10
- 239000004220 glutamic acid Substances 0.000 claims abstract description 10
- 239000000600 sorbitol Substances 0.000 claims abstract description 10
- 229920002627 poly(phosphazenes) Polymers 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims description 20
- 230000004151 fermentation Effects 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000011218 seed culture Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 abstract description 3
- 230000002087 whitening effect Effects 0.000 abstract description 2
- 230000002500 effect on skin Effects 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 2
- 229960001231 choline Drugs 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 235000008160 pyridoxine Nutrition 0.000 description 2
- 239000011677 pyridoxine Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
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- Cosmetics (AREA)
Abstract
The present invention relates to and a kind ofly with the addition of facial film of polyphosphazene polymer glutamic acid and preparation method thereof, belong to cosmetic skin care product field.Facial film of the present invention, comprises the component of following parts by weight: gamma-polyglutamic acid-5-20 part, collagen protein 0.05-5 part, vitamin C 0.05-0.5 part, Aloe juice 10-15 part, Margarita powder 10-20 part, Mel 2-5 part, sorbitol 1-5 part, distilled water 15-30 part.Facial film of the present invention has efficient moisture-retention, locks water lastingly, moistening skin, makes the skin effect such as refinement whitening more.
Description
Technical field
The present invention relates to and a kind ofly with the addition of facial film of polyphosphazene polymer glutamic acid and preparation method thereof, belong to cosmetic skin care product field.
Background technology
Facial film is a kind of cosmetics and skincare product spreading on human body face, take off after the 10-30 on the face in people minute that applies, its principle is exactly utilize facial film in the brief stay time of face, temporarily can isolate the air in environment residing for skin and pollution, the pore of skin expansion, promote the metabolism of sweat gland, the oxygen content of skin is made to increase, be conducive to the oil substances that skin gets rid of the metabolic product of epidermis cell and accumulation, moisture in facial film infiltrates the horny layer of epidermis, skin becomes soft, and skin naturally light is flexible.
Polyglutamic acid has extremely strong moisture retention, can form layer protecting film to prevent the evaporation of skin moisture on skin, it can penetrate into skin deep layer, recovers skin oneself moisture-keeping system.With advancing age with the aggravation of physical fatigue, weakening gradually from profit function of skin, even disappears, and the facial film that with the addition of polyglutamic acid can repair skin certainly moistens function.Therefore polyglutamic acid has for the skin of the mankind protected effect of well improving looks.
The invention provides a kind of new technique, make the facial film that with the addition of polyglutamic acid can become a kind of efficient moisture saver mask moistening, lock water.
Summary of the invention
The object of this invention is to provide a kind of efficient moisture-retention, lock water lastingly, moistening skin, make the facial film and preparation method thereof of skin refinement whitening more, the core technology of this facial film is the effective ingredient using the macromolecule such as polyglutamic acid and collagen protein as facial film.For achieving the above object, the present invention adopts following technical scheme.
Facial film of the present invention, comprises the component of following parts by weight: gamma-polyglutamic acid-5-20 part, collagen protein 0.05-5 part, vitamin C 0.05-0.5 part, Aloe juice 10-15 part, Margarita powder 10-20 part, Mel 2-5 part, sorbitol 1-5 part, distilled water 15-30 part.
The preparation method of described facial film comprises the following steps:
(1) gamma-polyglutamic acid-and Margarita powder are dissolved in the distilled water of 15-30 part, and stir, become thick liquid;
(2) joined in aforesaid liquid by Aloe juice, then add vitamin C, collagen protein, Mel, sorbitol, stirring and evenly mixing at ambient temperature, obtains mixed liquor;
(3) by mixed liquor under 50 DEG C of conditions dry 5 hours, the moisture saver mask that with the addition of polyphosphazene polymer glutamic acid can be obtained.
The bacterial strain of production high molecular gamma-polyglutamic acid-of the present invention is specially Bacillus licheniformis (Bacilluslicheniformis) CGMCC NO.3336(patent 101705260A).Utilize bacillus licheniformis to produce polyphosphazene polymer glutamic acid to comprise the following steps:
(1) actication of culture: be seeded on slant medium by Bacillus licheniformis CGMCC NO.3336 original strain, cultivates 16 hours in 37 DEG C, so activation 2-3 time, the inclined-plane seed that preparation is ripe;
(2) preparation of seed liquor: the inclined-plane seed after activation is inoculated in and is equipped with in the triangular flask of seed culture medium, 37 DEG C, 220rpm cultivates 16 little of exponential phase;
Seed culture medium consists of: glucose 30g/L, yeast extract 7g/L, tryptone 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.5g/L, pH7.2, insufficient section pure water is supplied;
(3) ferment tank: seed liquor step (2) prepared is inoculated in fermentation tank with the inoculum concentration of 5%, 30L ferment tank culture medium liquid amount is 15L, Preliminary fermentation temperature 38-40 DEG C, tank pressure 0.01-0.05MPa ventilation 0.5-1.0vvm, rotating speed 400-600rpm; As fermentation liquid OD
660when 0.550-0.600, control fermentation temperature to 30-35 DEG C, adjusting rotary speed to 200-350rpm, ventilation 1.5-2.0vvm, control pH at 7.2-7.5 to fermentation ends.
Described fermentation medium consists of: glucose 80g/L, sodium chloride 10g/L, MgSO
47H
2o1g/L, anhydrous calcium chloride 1g/L, FeSO
47H
2o0.02g/L, KCl0.5g/L, arginine 0.8g/L, histidine 0.5g/L, threonine 0.5g/L, methionine 0.4g/L, glutamine 0.5g/L, choline 0.31g/L, pyridoxol 0.001g/L, all the other are water.
(4) extraction of polyglutamic acid: centrifugally after fermentation ends remove thalline, then uses the ethanol of 5 times of supernatant volume, and precipitate with ethanol separates out polyglutamic acid, then redissolves in the distilled water of supernatant volume, utilizes spray drying process to prepare dry powder polyglutamic acid; Supernatant mobile phase is diluted the content of several times to gamma-polyglutamic acid-at 1-3g/L, detected its molecular weight by high performance liquid chromatography, molecular weight is 20 ten thousand to 200 ten thousand dalton simultaneously.
Beneficial effect:
Polyglutamic acid used in the present invention is efficient moisture-retention agent, and the glutamic acid after polyglutamic acid degraded is simultaneously nontoxic, biodegradable, to the nutritious high-end product of skin.And polyglutamic acid is applicable to all skins completely, and provides and be better than hyaluronic acid (HA) moistening effect;
The wherein effect of collagen protein: the effect that can reach degree of depth lock water and highly nourishing, makes skin water run through white, exquisiteness of compacting, high resilience; And the vitamin C added has scavenging free radicals and antioxidative function.
It is simple that the present invention prepares polyglutamic acid technological process, especially succinct effective extraction process, and this extraction process significantly can reduce production cost, also can reduce the loss rate of polyglutamic acid simultaneously, the final yieid of polyglutamic acid is improved; In addition, the method can obtain the polyglutamic acid of high molecular, for follow-up making moisture saver mask advantageously.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Embodiment 1
One, polyphosphazene polymer glutamic acid is prepared
(1) actication of culture: be seeded on slant medium by Bacillus licheniformis CGMCC NO.3336 original strain, cultivates 16 hours in 37 DEG C, so activation 2-3 time, the inclined-plane seed that preparation is ripe;
(2) preparation of seed liquor: the inclined-plane seed after a ring activation is inoculated in and is equipped with in the 500ml triangular flask of 50ml fluid medium, 37 DEG C, 220rpm cultivates 16 little of exponential phase;
Described seed culture medium consists of: glucose 30g/L, yeast extract 7g/L, tryptone 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.5g/L, pH7.2, insufficient section pure water is supplied;
(4) ferment tank: by the seed liquor in step (2) with 10% inoculum concentration be inoculated in fermentation tank, 30L ferment tank culture medium liquid amount is 15L, Preliminary fermentation temperature 38-40 DEG C, tank pressure 0.01-0.05Mpa ventilation 0.5-1.0vvm, rotating speed 400-600rpm; As fermentation liquid OD
660when 0.550-0.600, and control fermentation temperature 30-35 DEG C, adjusting rotary speed to 200-350rpm, ventilation 1.5-2.0vvm, control pH at 7.2-7.5 to fermentation ends.
Described fermentation medium consists of: glucose 80g/L, sodium chloride 10g/L, MgSO
47H
2o1g/L, anhydrous calcium chloride 1g/L, FeSO
47H
2o0.02g/L, KCl0.5g/L, arginine 0.8g/L, histidine 0.5g/L, threonine 0.5g/L, methionine 0.4g/L, glutamine 0.5g/L, choline 0.31g/L, pyridoxol 0.001g/L, all the other are water.
(4) extraction of polyglutamic acid: centrifugally after fermentation ends remove thalline, then uses the ethanol of 5 times of supernatant volume, and precipitate with ethanol separates out polyglutamic acid, is then redissolving the distilled water in supernatant volume, is utilizing spray drying process to prepare dry powder polyglutamic acid.Supernatant mobile phase is diluted the content of several times to gamma-polyglutamic acid-at 1-3g/L, detected by high-efficient liquid its molecular weight relative, molecular weight is 20 ten thousand to 200 ten thousand dalton simultaneously.
Two, facial film is prepared
Described facial film, comprises the component of following parts by weight: polyglutamic acid 5 parts; Collagen protein 0.05 part; Vitamin C 0.05 part; Aloe juice 10 parts; Margarita powder 10 parts; Mel 2 parts; Sorbitol 1 part;
Distilled water 15 parts.
The preparation method of this facial film comprises the following steps:
(1) polyglutamic acid and Margarita powder are dissolved in distilled water, and stir, become thick liquid;
(2) joined in aforesaid liquid by Aloe juice, then add vitamin C, collagen protein, Mel, sorbitol, stirring and evenly mixing at ambient temperature, obtains mixed liquor;
(3) by mixed liquor under 50 DEG C of conditions dry 5 hours, the moisture saver mask that with the addition of polyphosphazene polymer glutamic acid can be obtained.Embodiment 2
The preparation method of polyphosphazene polymer glutamic acid and the preparation method of facial film are substantially with embodiment 1;
Described facial film, comprises the component of following parts by weight: polyglutamic acid 12 parts; Collagen protein 2 parts; Vitamin C 0.25 part; Aloe juice 12 parts; Margarita powder 15 parts; Mel 2.5 parts; Sorbitol 3 parts; Distilled water 23 parts.
Embodiment 3
The preparation method of polyphosphazene polymer glutamic acid and the preparation method of facial film are substantially with embodiment 1;
Described facial film, comprises the component of following parts by weight: polyglutamic acid 20 parts; Collagen protein 5 parts; Vitamin C 0.5 part; Aloe juice 15 parts; Margarita powder 20 parts; Mel 5 parts; Sorbitol 5 parts; Distilled water 30 parts.
Claims (2)
1. the facial film of pbz polymer polyglutamic acid, is made up of the component of following parts by weight: gamma-polyglutamic acid-5-20 part, collagen protein 0.05-5 part, vitamin C 0.05-0.5 part, Aloe juice 10-15 part, Margarita powder 10-20 part, Mel 2-5 part, sorbitol 1-5 part, distilled water 15-30 part;
The preparation method of described facial film comprises the following steps:
(1) gamma-polyglutamic acid-and Margarita powder are dissolved in distilled water, and stir, become thick liquid;
(2) joined in aforesaid liquid by Aloe juice, then add vitamin C, collagen protein, Mel, sorbitol, stirring and evenly mixing at ambient temperature, obtains mixed liquor;
(3) by mixed liquor under 50 DEG C of conditions dry 5 hours, the moisture saver mask that with the addition of polyphosphazene polymer glutamic acid can be obtained;
The production method of described gamma-polyglutamic acid-comprises the following steps:
(1) actication of culture: be seeded on slant medium by Bacillus licheniformis CGMCC NO.3336 original strain, cultivates 16 hours in 37 DEG C, so activation 2-3 time, the inclined-plane seed that preparation is ripe;
(2) preparation of seed liquor: the inclined-plane seed after activation is inoculated in and is equipped with in the triangular flask of seed culture medium, 37 DEG C, 220rpm cultivates 16 little of exponential phase; Seed culture medium consists of: glucose 30g/L, yeast extract 7g/L, tryptone 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o 0.5g/L, pH 7.2, insufficient section pure water is supplied;
(3) ferment tank: seed liquor step (2) prepared is inoculated in fermentation tank with the inoculum concentration of 5%, 30L ferment tank culture medium liquid amount is 15L, Preliminary fermentation temperature 38-40 DEG C, tank pressure 0.01-0.05MPa, ventilation 0.5-1.0vvm, rotating speed 400-600rpm; As fermentation liquid OD
660when 0.550-0.600, control fermentation temperature to 30-35 DEG C, adjusting rotary speed to 200-350rpm, ventilation 1.5-2.0vvm, control pH at 7.2-7.5 to fermentation ends;
(4) extraction of gamma-polyglutamic acid-: centrifugally after fermentation ends remove thalline, then the ethanol of 5 times of supernatant volume is used, precipitate with ethanol separates out gamma-polyglutamic acid-, then redissolves in the distilled water of supernatant volume, utilizes spray drying process to prepare dry powder gamma-polyglutamic acid-; Supernatant mobile phase is diluted the content of several times to gamma-polyglutamic acid-at 1-3g/L, detected its molecular weight by high performance liquid chromatography, molecular weight is 20 ten thousand to 200 ten thousand dalton simultaneously.
2. the facial film of pbz polymer polyglutamic acid as claimed in claim 1, it is characterized in that, described facial film is made up of the component of following parts by weight: gamma-polyglutamic acid-12 parts; Collagen protein 2 parts; Vitamin C 0.25 part; Aloe juice 12 parts; Margarita powder 15 parts; Mel 2.5 parts; Sorbitol 3 parts; Distilled water 23 parts.
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| CN201310750171.1A CN103690396B (en) | 2013-12-25 | 2013-12-25 | Facial film of pbz polymer polyglutamic acid and preparation method thereof |
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| CN104087628A (en) * | 2014-04-28 | 2014-10-08 | 广西大学 | Method for reducing viscosity of gamma-polyglutamic acid fermentation liquid |
| CN104522542A (en) * | 2014-12-17 | 2015-04-22 | 刘长华 | Anti-acne mask |
| CN104498551A (en) * | 2014-12-23 | 2015-04-08 | 天津北洋百川生物技术有限公司 | Preparation method of macromolecule gamma-polyglutamic acid and shaving foam |
| US10471106B2 (en) | 2015-06-16 | 2019-11-12 | George D. Petito | Composition having hydrolyzed collagen and manuka honey |
| CN105603013A (en) * | 2015-12-11 | 2016-05-25 | 天津北洋百川生物技术有限公司 | Preparation method for gamma-polyglutamic acid, and hand cream and preparation method thereof |
| CN105603012A (en) * | 2015-12-11 | 2016-05-25 | 天津北洋百川生物技术有限公司 | Preparation method for gamma-polyglutamic acid, and moisturizing face cream and preparation method thereof |
| CN105968383B (en) * | 2016-04-05 | 2019-03-22 | 山东省药学科学院 | A kind of low molecular weight polyglutamic acid and the preparation method and application thereof |
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| CN107007508A (en) * | 2017-05-17 | 2017-08-04 | 太仓新亚逊生物科技有限公司 | A kind of whitening and moisturizing facial mask |
| CN107296784A (en) * | 2017-07-06 | 2017-10-27 | 天津杞源堂生物工程有限公司 | A kind of skin whitening, moisturizing fermentation Chinese wolfberry fruit liquid level film and preparation method thereof |
| CN108852988A (en) * | 2018-07-28 | 2018-11-23 | 高昕文 | A kind of old age face nursing facial mask liquid |
| CN110393677A (en) * | 2019-07-02 | 2019-11-01 | 广州黛莱美化妆品有限公司 | One kind is containing all-round facial mask of amino acid/collagen stage and preparation method thereof |
| CN110644232B (en) * | 2019-09-20 | 2021-10-12 | 南通大学 | Preparation method of natural antibacterial water-retention mask |
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| CN101416930A (en) * | 2008-12-22 | 2009-04-29 | 山东省生物药物研究院 | Face mask containing pullulan |
| CN102719501A (en) * | 2012-06-29 | 2012-10-10 | 天津北洋百川生物技术有限公司 | Method for producing polyglutamic acid |
| CN102871947A (en) * | 2012-10-29 | 2013-01-16 | 杨军 | Natural powdery facial mask |
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2013
- 2013-12-25 CN CN201310750171.1A patent/CN103690396B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416930A (en) * | 2008-12-22 | 2009-04-29 | 山东省生物药物研究院 | Face mask containing pullulan |
| CN102719501A (en) * | 2012-06-29 | 2012-10-10 | 天津北洋百川生物技术有限公司 | Method for producing polyglutamic acid |
| CN102871947A (en) * | 2012-10-29 | 2013-01-16 | 杨军 | Natural powdery facial mask |
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