CN103688936A - Compound bactericide containing eugenol and cyazofamid and application thereof - Google Patents
Compound bactericide containing eugenol and cyazofamid and application thereof Download PDFInfo
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- CN103688936A CN103688936A CN201310352835.9A CN201310352835A CN103688936A CN 103688936 A CN103688936 A CN 103688936A CN 201310352835 A CN201310352835 A CN 201310352835A CN 103688936 A CN103688936 A CN 103688936A
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- cyazofamid
- eugenol
- downy mildew
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Abstract
The invention discloses a compound bactericide containing eugenol and cyazofamid and an application thereof. The effective components comprise eugenol and cyazofamid with a mass ratio of 5:1-20:1. According to the invention, through indoor toxicity tests and field trials, the control effects of cyazofamid and eugenol single agents and the compound preparation on downy mildew are determined respectively, and an optimal ratio is determined to reach the purposes of improving control effects, decreasing chemical pesticide using amounts, and delaying drug resistance generation by pathogens.
Description
Technical field
The present invention relates to a kind of composite bactericide, in particular, what the present invention relates to is a kind of for preventing and treating biogenic and chemical bactericide composite bactericide and the application thereof of crop and fruit tree downy mildew.
Background technology
Downy mildew endangers various places all over the world, and damage to crops of a great variety as melon, vegetables, fruit tree etc., particularly downy mildew of garpe are the first diseases during current wine brewing, Table Grape are produced, and to grape, produce and cause serious economic loss.Not yet find at present immunological species or type, the improvement of downy mildew still be take to chemical control as main; All there is pesticide resistance in various degree in conventional chemical agent phenylamide (phenylamides is as metalaxyl etc.), carbamates (carbamate is as Propamocarb etc.), methoxy acrylic (stroblurins is as Fluoxastrobin etc.), the morpholine class (morpholine is as dimethomorph etc.) of in production, preventing and treating downy mildew; Chemical agent to use in a large number for a long time problems such as causing residue of pesticide, environmental pollution outstanding day by day, people and animals, environment are caused to significant damage.
Biogenic pesticide active ingredient is that nature exists, generally nontoxic, is easy to degraded, and in ecotope, accumulation toxicity is little, has Environmental compatibility, avoid or alleviated the negative effect that applied chemistry agricultural chemicals causes, and pathogen is difficult to develop immunity to drugs etc.; Although pure biogenic pesticide is environmentally friendly bactericide, owing to there is the problems such as under the complex environment of field, drug effect is unstable, cost of manufacture is high, cause it to be difficult to extensive use on producing.
Cyazofamid (cyazofamid) is sulphaguanidine azoles fungicide, and Oomycete fungi is had to strong bactericidal activity; To each vegetative stage of Pseudoperonospora cubensis history of life comprise sporangial formation, sporangial sprouting, ovum spore in formation, the release of zoospore, the growths of the movement of zoospore and mycelia etc. all have very high inhibition; The concentration for the treatment of of this medicament 40～50mg/L has outstanding preventive and therapeutic effect to downy mildew, and its using dosage is significantly lower than other bactericide; The cyazofamid mode of action is different from other bactericide, and the mutual pesticide resistance of nothing between other systemic fungicides; This reagent toxicity is low, has good toxicology character, to ecotope and crop and non-target organism safety.
Eugenol (eugenol) is a kind of aromatic compounds, is present in caryophyllus oil, cinnamon leaves oil, cassia oil, camphorated oil, nutmeg wet goods, can in caryophyllus oil, separate.Numerous research shows, volatile oil and composition thereof have many-sided biologically active, has biologically active and treatment, health-care efficacy at aspects such as anti-oxidant, antimicrobial, anti parasitic, antitumor, immunological regulation, analgesias.Enjoy in recent years the concern of clinician, scientific research circle and pesticide industry, by R&D institution and producer, with used as pesticides, applied.
Summary of the invention
The object of the present invention is to provide a kind of environmentally friendly, efficient, composite bactericide of containing eugenol and cyazofamid, this composite bactericide is had outstanding performance preventing and treating aspect downy mildew.
The present invention has inquired into the application in agriculture field of cyazofamid and eugenol and complex composition thereof, especially for the control of downy mildew.
The present invention is a kind of composite bactericide that contains cyazofamid and eugenol, and its active ingredient comprises eugenol and cyazofamid, and the quality proportioning of cyazofamid and eugenol is 5～20: 1, and best in quality proportioning is 11: 1.
Cyazofamid and the eugenol weight percentage in composite bactericide is respectively cyazofamid 1.5～6.0%, eugenol 0.075～0.3%, and surplus is conventional auxiliary material additive.
The application of complex preparation of the present invention in preventing and treating downy mildew.Cyazofamid and eugenol single dose all have certain control efficiency for preventing and treating downy mildew, and application cyazofamid and eugenol complex preparation are prevented and treated downy mildew, show obvious synergistic effect.There is following advantage:
1, there is synergistic effect.Cyazofamid and eugenol all show synergistic effect in the ratio range of 5: 1 to 20: 1, and wherein proportioning synergy in 11: 1 is the most obvious, and coefficient of synergism is 1.67.
2, the composite amount of application that reduces medicament.When use by 36.0mg/L～45.0mg/L concentration in field, not only improved the control efficiency to downy mildew, also can reduce by 10.0%～28.0% pesticide dosage.
3, cyazofamid is different with the eugenol mode of action, is difficult for making germ to develop immunity to drugs after composite.
The present invention is by Toxicity Determination and field trial, cyazofamid, eugenol single dose and the control efficiency of complex preparation to downy mildew have been measured respectively, established optimal proportion, to reach, improved preventive effect, reduce chemical pesticide consumption, slow down the object that germ develops immunity to drugs.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
1 indoor biological activity determination test
Experiment target is designated as downy mildew of garpe (Plasmopara viticola), and sample picks up from field.Test material selects grape susceptible variety Cabernet Sauvignon potted plant, 4 leaf positions, leaf position-6 from up to down, and clip same area, growing way be consistent, with wet cotton balls, wrap up petiole is placed in culture dish, and moisturizing is standby.
1.1 test medicine
The former medicine of 95% cyazofamid; 0.3% eugenol soluble concentrate.
1.2 test method
Sporangia suspension preparation: select incidence of leaf, with 4 ℃ of distillation washing lower blade back side Pseudoperonospora cubensis sporangiums, (concentration is controlled at 1-6 * 10 to be made into suspension
6individual sporangium/ml), deposit at 4 ℃ standby.
Chemicals treatment: 0.3% eugenol soluble concentrate and the former medicine of cyazofamid, be made into maximum concentration mother liquor.Cyazofamid, eugenol single dose and each compounded combination all arrange 7 concentration gradients.Liquid is evenly sprayed in vacuum side of blade, after liquid natural air drying, each is processed to blade and blade and go up dorsad, by being emitted in moisture preservation box after marks for treatment.Test is not established and is made blank containing the processing of medicament.With Ye Panfa (Schwinn etc., 1982), test.
1.3 investigation method
According to leaf dish severity Scaling method, investigate the incidence of each processing.Leaf dish severity Scaling method is as follows: 0 grade: without scab; 1 grade: lesion area accounts for below 5% of whole leaf area; 3 grades: lesion area accounts for the 6%-25% of whole leaf area; 5 grades: lesion area accounts for the 26%-50% of whole leaf area; 7 grades: lesion area accounts for the 51%-75% of whole leaf area; 9 grades: lesion area accounts for the more than 76% of whole leaf area.Record disease leaf rate and severity, calculate disease index and preventive effect (%).
The drug concentration logarithm value of take is done regression analysis as independent variable x, the probability value of control efficiency of take as dependent variable y, calculates the EC of each medicament
50and 95% confidence limit.Press Wadley method, calculate coefficient of synergism (SR); The synergy type of evaluating pharmacy mix according to coefficient of synergism (SR), SR < 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR > 1.5 is synergistic effect.
1.4 result of the test
Result of the test and statistics are as table 1, and result shows that cyazofamid, eugenol single dose all show good indoor biologically active, EC to Pseudoperonospora cubensis
50be respectively 6.182mg/L and 0.492mg/L.Cyazofamid and eugenol are 5～20: in 1 ratio range, all show synergistic effect, wherein proportioning synergy in 11: 1 is the most remarkable, and coefficient of synergism is 1.67.
The best proportioning the selection result of table 1 cyazofamid and eugenol table
2 field efficacy evaluation tests
The cyazofamid going out according to lab screening and eugenol optimum compound proportion example 11: 1, made 3.6% eugenol cyazofamid composite bactericide, wherein containing 0.3% eugenol and 3.3% cyazofamid.With 2000 times of (50.0mg/L) liquid of 100 grams per liter cyazofamid suspending agent, 500 times of (6.0mg/L) liquid of 0.3% eugenol soluble concentrate medicament in contrast, carried out field control effectiveness test.
2.1 subjects, crop
Subjects is downy mildew of garpe (Plasmopara viticola), and test kind is muscat grape.The sheet trellis type cultivation of test garden, age of tree life in 5 years, downy mildew over the years occurs general and even.Duration of test (12～September 2 August) grape breeding time is the young sprout pumping phase.The cultivation condition of all experimental plots (soil types, fertilising, growing stage, seeding row spacing etc.) and tree body growing way homogeneous, and consistent with local cultivation step.
2.2 test method
3.6% eugenol cyazofamid composite bactericide, the preparation of Institute of Plant Protection, academy of agricultural sciences, Shandong Province.Contrast medicament 100 grams per liter cyazofamid suspending agents; 0.3% eugenol soluble concentrate, test Treatment Design is as table 2.
Every community grape strain tree is 10 strains, repeats 4 times.Spraying equipment adopts SDR-120 type power spraye, the dispenser first of downy mildew initial stage of origination, and interval sprays once for 7 days, sprays altogether 3 times.The concrete dispenser date is on August 12nd, 2012, August 19 and August 26.
Table 2 reagent agent experimental scheme
Test is investigated 2 times altogether, investigation state of an illness radix before dispenser, and 7d investigation protection effect after last medication, control time is August 12, September 2.10 young sprouts of the every community investigation of investigation method, every tip is investigated whole blades from top to bottom, records respectively total number of sheets, the sick number of sheets and severity.Severity grade scale: 0 grade: without scab; 1 grade: lesion area accounts for below 5% of whole leaf area; 3 grades: lesion area accounts for 6%～25% of whole leaf area; 5 grades: lesion area accounts for 26%～50% of whole leaf area; 7 grades: lesion area accounts for 51%～75% of whole leaf area; 9 grades: lesion area accounts for the more than 75% of whole leaf area, calculate disease index and preventive effect.
2.3 result of the test
Result of the test and analysis are as table 3, each processing of 3.6% eugenol cyazofamid composite bactericide 30.0～45.0mg/L all shows good preventive effect, 36.0～45.0mg/L respectively processes preventive effect more than 90%, is significantly higher than the preventive effect of 100 grams per liter cyazofamid suspending agent 50.0mg/L and 0.3% eugenol soluble concentrate 6.0mg/L.
This result of the test shows that SYP-3375 and cyazofamid, by the mixed ratio of 11: 1, make 3.6% eugenol cyazofamid built agent remarkable to preventing and treating downy mildew of garpe effect.
Duration of test, does not find that grape has poisoning or other anomaly.
Table 3 3.6% eugenol cyazofamid compositional bactericidal agent for preventing and treating downy mildew field test results
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (3)
1. a composite bactericide that contains cyazofamid and eugenol, is characterized in that, its active ingredient comprises eugenol and cyazofamid, and the quality proportioning of cyazofamid and eugenol is 5～20: 1.
2. composite bactericide as claimed in claim 1, is characterized in that, the best in quality proportioning of cyazofamid and eugenol is 11: 1.
3. the application of composite bactericide as claimed in claim 1 or 2, is characterized in that, the application of the composite bactericide that contains cyazofamid and eugenol in preventing and treating downy mildew.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108477195A (en) * | 2018-04-10 | 2018-09-04 | 山东省烟台市农业科学研究院 | The complex composition and its application of cymoxanil and eugenol |
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| CN101438703A (en) * | 2008-12-18 | 2009-05-27 | 山东营养源食品科技有限公司 | Sterilizing compositional composition containing eugenol |
| CN101584316A (en) * | 2009-05-27 | 2009-11-25 | 江苏省农业科学院 | Soluble eugenol solvent, preparation method and application thereof |
| CN101632391A (en) * | 2008-07-25 | 2010-01-27 | 刘志刚 | Development of novel indoor acaricide |
| CN101971812A (en) * | 2010-11-08 | 2011-02-16 | 陕西汤普森生物科技有限公司 | Eugenol-containing germicidal composition |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101632391A (en) * | 2008-07-25 | 2010-01-27 | 刘志刚 | Development of novel indoor acaricide |
| CN101438703A (en) * | 2008-12-18 | 2009-05-27 | 山东营养源食品科技有限公司 | Sterilizing compositional composition containing eugenol |
| CN101584316A (en) * | 2009-05-27 | 2009-11-25 | 江苏省农业科学院 | Soluble eugenol solvent, preparation method and application thereof |
| CN101971812A (en) * | 2010-11-08 | 2011-02-16 | 陕西汤普森生物科技有限公司 | Eugenol-containing germicidal composition |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477195A (en) * | 2018-04-10 | 2018-09-04 | 山东省烟台市农业科学研究院 | The complex composition and its application of cymoxanil and eugenol |
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