CN108576057A - A kind of bactericide compounded composition of biogas slurry-and its application - Google Patents
A kind of bactericide compounded composition of biogas slurry-and its application Download PDFInfo
- Publication number
- CN108576057A CN108576057A CN201810138273.0A CN201810138273A CN108576057A CN 108576057 A CN108576057 A CN 108576057A CN 201810138273 A CN201810138273 A CN 201810138273A CN 108576057 A CN108576057 A CN 108576057A
- Authority
- CN
- China
- Prior art keywords
- biogas slurry
- fungicide
- bactericide
- compounded composition
- bactericidal agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 68
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 41
- 239000000203 mixture Substances 0.000 title claims abstract description 29
- 239000002002 slurry Substances 0.000 claims abstract description 71
- 239000000417 fungicide Substances 0.000 claims abstract description 36
- 230000000855 fungicidal effect Effects 0.000 claims abstract description 34
- 241000233679 Peronosporaceae Species 0.000 claims abstract description 31
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 23
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 23
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 23
- 235000013311 vegetables Nutrition 0.000 claims abstract description 17
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical class NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- DGJMPUGMZIKDRO-UHFFFAOYSA-N cyanoacetamide Chemical class NC(=O)CC#N DGJMPUGMZIKDRO-UHFFFAOYSA-N 0.000 claims abstract description 8
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 claims abstract description 7
- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims abstract description 7
- 150000004841 phenylimidazoles Chemical class 0.000 claims abstract description 7
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims abstract description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000219104 Cucurbitaceae Species 0.000 claims abstract description 3
- 244000241257 Cucumis melo Species 0.000 claims abstract 2
- 240000006365 Vitis vinifera Species 0.000 claims abstract 2
- QNBTYORWCCMPQP-JXAWBTAJSA-N (Z)-dimethomorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(Cl)=CC=1)=C/C(=O)N1CCOCC1 QNBTYORWCCMPQP-JXAWBTAJSA-N 0.000 claims description 33
- 239000005761 Dimethomorph Substances 0.000 claims description 33
- 201000010099 disease Diseases 0.000 claims description 33
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 33
- 230000002265 prevention Effects 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 claims description 7
- 239000005800 Kresoxim-methyl Substances 0.000 claims description 7
- ZOTBXTZVPHCKPN-HTXNQAPBSA-N kresoxim-methyl Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC=C1C ZOTBXTZVPHCKPN-HTXNQAPBSA-N 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 230000001681 protective effect Effects 0.000 claims description 6
- XERJKGMBORTKEO-VZUCSPMQSA-N (1e)-2-(ethylcarbamoylamino)-n-methoxy-2-oxoethanimidoyl cyanide Chemical group CCNC(=O)NC(=O)C(\C#N)=N\OC XERJKGMBORTKEO-VZUCSPMQSA-N 0.000 claims description 5
- 239000005756 Cymoxanil Substances 0.000 claims description 5
- 239000005807 Metalaxyl Substances 0.000 claims description 5
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 claims description 5
- 150000003217 pyrazoles Chemical class 0.000 claims description 5
- BKBSMMUEEAWFRX-NBVRZTHBSA-N (E)-flumorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(F)=CC=1)=C\C(=O)N1CCOCC1 BKBSMMUEEAWFRX-NBVRZTHBSA-N 0.000 claims description 4
- KWLVWJPJKJMCSH-UHFFFAOYSA-N 2-(4-chlorophenyl)-N-{2-[3-methoxy-4-(prop-2-yn-1-yloxy)phenyl]ethyl}-2-(prop-2-yn-1-yloxy)acetamide Chemical compound C1=C(OCC#C)C(OC)=CC(CCNC(=O)C(OCC#C)C=2C=CC(Cl)=CC=2)=C1 KWLVWJPJKJMCSH-UHFFFAOYSA-N 0.000 claims description 4
- 239000005754 Cyazofamid Substances 0.000 claims description 4
- 239000005797 Iprovalicarb Substances 0.000 claims description 4
- NWUWYYSKZYIQAE-ZBFHGGJFSA-N L-(R)-iprovalicarb Chemical compound CC(C)OC(=O)N[C@@H](C(C)C)C(=O)N[C@H](C)C1=CC=C(C)C=C1 NWUWYYSKZYIQAE-ZBFHGGJFSA-N 0.000 claims description 4
- 239000005802 Mancozeb Substances 0.000 claims description 4
- 239000005804 Mandipropamid Substances 0.000 claims description 4
- 239000005821 Propamocarb Substances 0.000 claims description 4
- 239000005823 Propineb Substances 0.000 claims description 4
- 239000005857 Trifloxystrobin Substances 0.000 claims description 4
- YXKMMRDKEKCERS-UHFFFAOYSA-N cyazofamid Chemical group CN(C)S(=O)(=O)N1C(C#N)=NC(Cl)=C1C1=CC=C(C)C=C1 YXKMMRDKEKCERS-UHFFFAOYSA-N 0.000 claims description 4
- WZZLDXDUQPOXNW-UHFFFAOYSA-N propamocarb Chemical group CCCOC(=O)NCCCN(C)C WZZLDXDUQPOXNW-UHFFFAOYSA-N 0.000 claims description 4
- KKMLIVYBGSAJPM-UHFFFAOYSA-L propineb Chemical compound [Zn+2].[S-]C(=S)NC(C)CNC([S-])=S KKMLIVYBGSAJPM-UHFFFAOYSA-L 0.000 claims description 4
- ONCZDRURRATYFI-TVJDWZFNSA-N trifloxystrobin Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1CO\N=C(/C)C1=CC=CC(C(F)(F)F)=C1 ONCZDRURRATYFI-TVJDWZFNSA-N 0.000 claims description 4
- -1 carboxylic acid Acid amide Chemical class 0.000 claims description 3
- AMHNZOICSMBGDH-UHFFFAOYSA-L zineb Chemical compound [Zn+2].[S-]C(=S)NCCNC([S-])=S AMHNZOICSMBGDH-UHFFFAOYSA-L 0.000 claims description 3
- FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 claims 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims 1
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- 239000005869 Pyraclostrobin Substances 0.000 description 31
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 description 30
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- 239000000126 substance Substances 0.000 description 6
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- 206010059866 Drug resistance Diseases 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241000233654 Oomycetes Species 0.000 description 4
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
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- 239000003921 oil Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 239000004563 wettable powder Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 238000012271 agricultural production Methods 0.000 description 3
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- 241001281805 Pseudoperonospora cubensis Species 0.000 description 2
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- LQAQMOIBXDELJX-UHFFFAOYSA-N 2-methoxyprop-2-enoic acid Chemical class COC(=C)C(O)=O LQAQMOIBXDELJX-UHFFFAOYSA-N 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
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- 208000031888 Mycoses Diseases 0.000 description 1
- PXUQTDZNOHRWLI-JJYSZZJESA-O Oenin Natural products O(C)c1c(O)c(OC)cc(-c2c(O[C@H]3[C@@H](O)[C@H](O)[C@H](O)[C@@H](CO)O3)cc3c(O)cc(O)cc3[o+]2)c1 PXUQTDZNOHRWLI-JJYSZZJESA-O 0.000 description 1
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- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
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- 239000003895 organic fertilizer Substances 0.000 description 1
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- 125000001741 organic sulfur group Chemical group 0.000 description 1
- IBSNKSODLGJUMQ-SDNWHVSQSA-N picoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC(C(F)(F)F)=N1 IBSNKSODLGJUMQ-SDNWHVSQSA-N 0.000 description 1
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- JOYRKODLDBILNP-UHFFFAOYSA-N urethane group Chemical group NC(=O)OCC JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the complex composition technical fields of fungicide, and in particular to a kind of bactericide compounded composition of biogas slurry and its application.Active ingredient of the present invention includes components I and compositionⅱ, the components I be biogas slurry, the compositionⅱ be carboxylic acid amide series bactericidal agent, methoxy acrylic bactericide, phenyl amide series bactericidal agent, carbamate fungicide, cyanoacetamide series bactericidal agent, organic sulfurcontaining fungicide, phenylimidazole series bactericidal agent it is any.The complex composition containing biogas slurry and fungicide of the present invention has apparent synergistic effect, not only reduce pesticide usage amount, reduce residual quantity of the pesticide on crop, alleviate environmental pollution, crop yield can also be increased, and improve crop quality, it is notable to the crops downy mildew control effect such as grape, melon vegetables, brassicaceous vegetable, cucurbitaceae vegetable, leguminous vegetable.
Description
Technical field
The invention belongs to the complex composition technical fields of fungicide, and in particular to a kind of bactericide compounded combination of biogas slurry-
Object and its application.
Background technology
In the real process of agricultural production, long-term a large amount of, single abuse chemical bactericide so that the drug resistance of pathogenic bacteria
Constantly reinforce, persticide residue increases.Different cultivars ingredient is compounded, and is to prevent the very common method of resistance pathogenic bacteria.Through
It crosses the study found that biogas slurry and the bactericide compounded prevention for preventing downy mildew of garpe have not been reported.
For the downy mildew of the crops such as effective prevention and control grape, the field rate of chemical bactericide is reduced, the application is by biogas slurry
It is compounded with common fungicide, through indoor and outdoor tests, has filtered out the optimum proportioning of composite bactericide, synergy is notable, favorably
It reduces environmental pollution in realization, control pathogenic bacteria resistance occurrence and development.
Invention content
For the problems of the prior art, a kind of bactericide compounded composition of biogas slurry-of present invention offer and its application, for
The prevention of crop downy mildew has apparent synergistic effect, and mitigates the pollution to environment and pesticide residue.
To achieve the above object, the present invention is achieved by the following technical solutions：
A kind of bactericide compounded composition of biogas slurry-of present invention offer and its application, are characterized in that：Active ingredient packet
Components I and compositionⅱ are included, the components I is biogas slurry, and the compositionⅱ is carboxylic acid amide series bactericidal agent, methoxy acrylic
Fungicide, phenyl amide series bactericidal agent, carbamate fungicide, cyanoacetamide series bactericidal agent, organic sulfurcontaining fungicide, benzene
Base imidazoles series bactericidal agent it is any.
Further, the weight proportion of the components I and compositionⅱ is 100:1-1:50.
Further, the weight proportion of the components I and compositionⅱ is 20:1-5:1.
Further, the weight proportion of the components I and compositionⅱ is 10:1-1:1.
Further, the carboxylic acid amide series bactericidal agent is dimethomorph, flumorph, iprovalicarb, mandipropamid
It is any.
Further, the methoxy acrylic bactericide is ZEN 90160, pyrazoles ether bacterium, kresoxim-methyl, trifloxystrobin
It is any.
Further, the phenyl amide series bactericidal agent is metalaxyl.
Further, the carbamate fungicide is Propamocarb.
Further, the cyanoacetamide series bactericidal agent is cymoxanil.
Further, the phenylimidazole class protective fungicide is cyazofamid.
Further, organic sulfurcontaining fungicide be Mancozeb, Propineb, Carbatene it is any.
The present invention also provides a kind of applications of the bactericide compounded composition of biogas slurry-, are characterized in that, use the present invention
The bactericide compounded composition of any biogas slurry-, component I dilute 300-500 times, and the component II of 0.5-50% dilutes 2000-
5000 times are applied in the prevention of crop disease.
Further, the crop is in grape, melon vegetables, brassicaceous vegetable, cucurbitaceae vegetable, leguminous vegetable
It is one or more of.
Further, the disease is downy mildew.
Compared with prior art, the present invention it is beneficial in that：The compounding group containing biogas slurry and fungicide of the present invention
Closing object has apparent synergistic effect, not only reduces pesticide usage amount, reduces residual quantity of the pesticide on crop, alleviate
Environmental pollution, additionally it is possible to increase crop yield, and improve crop quality, to grape, melon vegetables, brassicaceous vegetable, cucurbit
The crops downy mildew control effect such as section vegetables, leguminous vegetable is notable.
1, there is synergistic effect.Such as both biogas slurry and pyraclostrobin are with 20：1 to 5：It is showed in 1 ratio range
Go out synergistic effect, wherein with 14.81：1 proportioning synergy is maximum；Both biogas slurry and dimethomorph are with 10：1 to 1：1 proportioning model
Enclose it is interior show synergistic effect, wherein with 7.41：1 proportioning synergy is maximum.
2, the bactericide compounded composition of biogas slurry-can reduce Pesticide use amount 10%~20%, effectively reduce environment dirt
Dye and pesticide residue.
3, biogas slurry and bactericide compounded reduces use cost, alleviates pollution to environment, delayed downy mildew of garpe
The generation of pathogenic bacteria drug resistance, the service life for increasing pesticide.
4, biogas slurry has effects that foliar fertilizer, can improve vintage, enhances tree vigo(u)r, improves grape quality, is worth
It is promoted and applied in agricultural production.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, it is measured below in conjunction with laboratory experiment, field
The evaluation of pesticide effectiveness, the present invention is described in further detail for security performance measurement.
Complex composition active ingredient provided by the invention includes components I and compositionⅱ, and the components I is chicken manure biogas slurry,
The compositionⅱ is carboxylic acid amide series bactericidal agent, methoxy acrylic bactericide, phenyl amide series bactericidal agent, carbamic acid
Esters fungicide, cyanoacetamide series bactericidal agent, organic sulfurcontaining fungicide, phenylimidazole series bactericidal agent it is any, for components I
The substance performance chosen with compositionⅱ is described below.
Biogas slurry is the liquid organic fertilizer that a kind of nutrient has both quick-acting features comprehensively, organic matter, a great number of elements (N, P, K etc.),
Micro- (B, Mn etc.) equal size is abundant, and effectiveness is mainly reflected in coordinate plant growth, provides nutrient and disease and insect resistance etc.
Aspect can develop into a kind of effective means substituting chemical pesticide control certain plants disease.Biogas slurry is with chicken in this research
Excrement is the commercial goods " newly strengthening state " of raw material, can be bought on the market.
Downy mildew of garpe (grape downy mildew) is caused by the uniaxial mould Plasmopara viticola of grape life
A kind of worldwide disease, all viny regions all over the world.Main harm blade can also infect the childrens such as tender tip, inflorescence, young fruit
Tender tissue.It is almost distributed in each viny region in China, the plant disease epidemic time, young fruit is covered with the mould layer of white, falls off, sick leaf scorch
Withered caducous, sick tip distortion, depauperation is very big to tree vigo(u)r and yield effect, seriously affects grape growth and fruit yield and product
Matter causes heavy economic losses to grape industry.
Carboxylic acyloxy amine (Carboxylicacidamides, CAAs) fungicide be after phenyl amide series bactericidal agent,
The systemic fungicide for leading one class preventing and control oomycetes disease to be used in production at present has plant Oomycete disease excellent
Protection and therapeutic effect, and with phenyl amide series bactericidal agent no interactions drug resistance, have become at present in order to countries in the world pesticide public affairs
Take charge of one of the hot spot of research.Carboxylic acid amide series bactericidal agent has dimethomorph (dimethomorph), flumorph at present
(flumorph), the commercial varieties such as iprovalicarb (iprovalicarb) and mandipropamid (mandipropamid), have become
The important medicament of Peronospora and Phytophthora fungal disease is prevented for China.
Methoxy acrylic bactericide is a kind of low toxicity, high activity, broad spectrum activity, systemic fungicide, have protect,
Treatment, eradicant action.They have good activity for Ascomycetes, Basidiomycetes, deuteromycetes and Oomycete etc..Such
Fungicide is a kind of mitochondrial respiratory inhibitor, inhibits mitochondrial respiratory by preventing between cytochrome b and c1 electron transmission
Effect, prevents mitochondria from generating and providing the required energy of cell eubolism (ATP), eventually leads to cell death.Mesh
It is preceding in the market be used for prevent grape disease methoxy acrylic product mainly have ZEN 90160 (picoxystrobin),
Pyrazoles ether bacterium (Pyraclostrobin), kresoxim-methyl (Kresoxim-methyl), trifloxystrobin (Trifloxystrobin) etc..
Metalaxyl (metalaxyl) belongs to phenylamide systemic fungicide, has protection and therapeutic effect, has low
Malicious, selectivity by force, only has the characteristics that oomycetes efficient.Metalaxyl is microbial more to Pseudoperonospora cubensis, phytophthora germ and maize ear rot
Kind crop downy mildew, the phytophthora root rot of gourd, fruit and vegetable class and the downy mildew of millet are effective.
Propamocarb (ProPamocarb) is the representative products of carbamate fungicide, has uptake and translocation effect.It is logical
Cross inhibit germ cell film component phosphatide and aliphatic acid biosynthesis, to inhibit mycelia grow and sporangium formation and
Spore germination.Main prevention downy mildew, phytophthora, rotten mould, disk obstruct the germ plant diseases such as mould, silk capsule is mould, and are given birth to crop
With certain stimulation.It is notable to grape, vegetable downy mildew preventive effect.
Cymoxanil (cymoxanil) belongs to cyanoacetamide series bactericidal agent, there is the ability of local absorbability and short distance conduction, right
Crop has protective effect and certain therapeutic effect.Cymoxanil is by inhibiting spore germination to be mainly used for kill pathogen
Prevent the downy mildew of the crops such as cucumber, grape.
Cyazofamid (cyazofamid) is phenylimidazole class protective fungicide, to Oomycete pathogen such as phytophthora, frost
Mould, false downy mildew, pythium spp etc. have very high active function, and the mechanism of action is by active ingredient and phytopathogen
The combination of cell mitochondrial inner membrance hinders electron transmission in film, interfering energy supply, to play the role of killing pathogen.
Organic sulfurcontaining fungicide is generally non-interior suction protective fungicide, has good protective effect, broad-spectrum right
The bacteriums such as the fungies such as mastigomycetes, sac fungus, basidiomycetes and Fungi Imperfecti and erwinia, Xanthomonas campestris, Pseudomonas alba have life
Object activity.Common organic sulfurcontaining fungicide mainly has Mancozeb (Mancozeb), Propineb on fruit tree in production
(propineb), Carbatene (metiram) etc..
Embodiment 1
Toxicity Determination is screened with compound proportion
1.1 reagent agent
Component I chicken manure biogas slurries derive from the Shandong people and biotech inc's product；Pyrrole is respectively adopted in component II
For azoles kresoxim-methyl, dimethomorph, 250 grams per liter pyraclostrobin missible oils of selection derive from company of BASF European Co., Ltd；Choosing
The 50% dimethomorph wettable powder taken derives from BASF European Co., Ltd.
1.2 Indoor antibacterial determinations of activity
1.2.1 the configuration of sporangia suspension
The new fresh grape disease leaf for taking field natural occurrence after rinsing the sporangium on net surface with tap water, is placed in 25 DEG C of items
Moisturizing for 24 hours, cultivates fresh sporangium under part.With clean writing brush by Fresh spores capsule brush in sterile distilled water, be made into spore
Ascus suspension.Sporangia suspension concentration is adjusted under the microscope to 5 × 108A/L is measured for testing.
1.2.2 experimental design
It is respectively 100 that experiment, which sets biogas slurry single dose, pyraclostrobin single dose, biogas slurry and pyraclostrobin weight proportion,：1、50：
1、20：1、15：1、10：1、5：1、1：1、1：5、1：10、1：20、1：50, it compares as clear water.
1.2.3 inoculation method
Leaf disk method is taken in experiment, will be natural after the disease-free corresponding liquid of 4~5 blades sprinkling of health on the new branch of grape
It dries, the leaf dish for taking diameter 1cm is beaten with card punch, blade back is placed in the plate for being covered with sterile moistening filter paper, is put per ware up
Set 11 leaf dishes；20 μ L sporangia suspensions to be dripped in leaf dish center, is placed in bio-incubator and cultivates, temperature is maintained at 25 DEG C,
Humidity is maintained at 50% or so, 12h illumination.Blade spraying equivalent clear water is compareed, is dried, the bacterium solution of dropwise addition equivalent after leaf dish is beaten.
Often processing repeats 4 wares.Incidence is investigated after 57d.
1.2.4 statistical method
Disease investigation uses 6 grades of Recording Methods, and the percentage that leaf dish area is accounted for according to lesion area divides sick grade：It is 0 grade, disease-free
Spot；1 grade, lesion area accounts for 5% or less entire leaf area；3 grades, lesion area accounts for the 6%~25% of entire leaf area；5 grades,
Lesion area accounts for the 26%~50% of entire leaf area；7 grades, lesion area accounts for the 51%~75% of entire leaf area；9 grades, disease
Spot area accounts for 75% or more of entire leaf area.
Calculate the inhibiting rate of disease index and each drug concentrations to Plasmopara viticola.
Disease index=Σ (the disease numbers of sheets at different levels × opposite value of series)/total leaf dish number/9 × 100 of investigation
Bacteriostasis rate (%)=(control disease index-processing disease index)/control disease index × 100
1.3 interpretation of result
The Toxicity Determination result shows：Good inhibition effect is shown in two kinds of single dose rooms to Plasmopara viticola
Fruit, bacteriostasis rate are respectively 40.37% and 82.42%.Biogas slurry and pyraclostrobin amine 5：1、10：1、15：1、20:1 is mixed to Portugal
Grape Pseudoperonospora cubensis bacteriostasis rate is respectively 88.52%, 92.13%, 96.07%, 90.61%, and synergy is very notable, biogas slurry and pyrazoles
Kresoxim-methyl 15：1 is optimum proportioning.Biogas slurry and pyraclostrobin other proportioning is mixed is less than to Plasmopara viticola bacteriostasis rate or phase
When in pyraclostrobin single dose, no notable synergistic effect.Biogas slurry and all proportionings of pyraclostrobin are mixed to Plasmopara viticola
Bacteriostasis rate is superior to biogas slurry single dose.
1 biogas slurry of table and the 250 mixed indoor virulences to Plasmopara viticola of grams per liter pyraclostrobin
| Processing | Disease refers to | Bacteriostasis rate (%) |
| (biogas slurry：Pyraclostrobin) 100:1 | 2.67 | 78.57c |
| 50:1 | 2.31 | 81.46c |
| 20:1 | 1.17 | 90.61b |
| 15:1 | 0.49 | 96.07a |
| 10:1 | 0.98 | 92.13ab |
| 5:1 | 1.43 | 88.52b |
| 1:1 | 2.07 | 83.39c |
| 1:5 | 2.39 | 80.82c |
| 1:10 | 2.58 | 79.29c |
| 1:20 | 3.17 | 74.56cd |
| 1:50 | 3.55 | 71.51d |
| 5mg/L pyraclostrobins | 2.19 | 82.42c |
| 0.1% biogas slurry | 7.43 | 40.37e |
| Clear water compares | 12.46 | —— |
It is 8.89 that biogas slurry is advanced optimized with pyraclostrobin proportioning：1、10.0：1、14.81：1、16.67：1, four pairs
The bacteriostasis rate of downy mildew of garpe is followed successively by 93.17%, 92.48%, 97.83%, 94.59%, biogas slurry and pyraclostrobin
14.81：1 is optimum proportioning.
Pyraclostrobin in said determination is replaced with dimethomorph, biogas slurry and dimethomorph 10：1、5：1、2：1、1：1 is mixed
With equally showing as synergistic effect, biogas slurry and dimethomorph 10：1 is optimum proportioning.Experimental data is shown in Table 2.
2 biogas slurry of the table indoor virulence to Plasmopara viticola mixed with 50% dimethomorph
| Processing | Disease refers to | Bacteriostasis rate (%) |
| (biogas slurry：Dimethomorph) 50:1 | 3.45 | 72.31d |
| 20:1 | 2.56 | 79.45c |
| 10:1 | 0.76 | 93.90a |
| 5:1 | 1.17 | 90.61a |
| 2:1 | 1.89 | 84.83b |
| 1:1 | 2.36 | 81.06bc |
| 1:2 | 2.63 | 78.89c |
| 1:5 | 2.98 | 76.08c |
| 1:10 | 3.25 | 73.92cd |
| 1:20 | 3.68 | 70.47d |
| 1:50 | 4.05 | 67.50d |
| 10mg/L dimethomorphs | 2.78 | 77.69c |
| 0.1% biogas slurry | 7.43 | 40.37e |
| Clear water compares | 12.46 | —— |
It is 4.44 that biogas slurry is advanced optimized with dimethomorph proportioning：1、5.0：1、7.41：1、8.33：1, four to oenin
The bacteriostasis rate of mildew is followed successively by 91.94%, 91.02%, 96.27%, 94.33%, biogas slurry and dimethomorph 7.41：1 is best
Proportioning.
Embodiment 2
Field efficacy evaluation test
Field trial sets following processing：It is 8.89 that biogas slurry is advanced optimized with pyraclostrobin proportioning：1、10.0：1、
14.81：1、16.67：1, with 4000 times of 250 grams per liter pyraclostrobin missible oil, biogas slurry 300 medicament as a contrast again；Biogas slurry and alkene
It is 4.44 that morpholide proportioning, which advanced optimizes,：1、5.0：1、7.41：1、8.33：1, with 50% dimethomorph wettable powder 2000
Times, the medicament as a contrast again of biogas slurry 300, while setting blank control processing.
2.1 test method
(1) selection of subjects, crop and kind
Subjects are downy mildew of garpe, and test variety is Cabernet Gernischt.
(2) arable farming and environmental condition
It tests and has carried out cell controlling experiment in Peng Lai tidewater Vinery in the 7-8 months in 2017, grape variety is snake
Long Zhu, the long-term region of disease of downy mildew, sandy loam matter, rich water is good, and management level is higher, grape seeding row spacing 1.0m × 1.5m, examination
Positive value fruit expanding period during testing carries out controlling experiment early period in downy mildew of garpe morbidity.
(3) reagent agent
Component I chicken manure biogas slurries derive from the Shandong people and biotech inc's product；Pyrrole is respectively adopted in component II
For azoles kresoxim-methyl, dimethomorph, 250 grams per liter pyraclostrobin missible oils of selection derive from company of BASF European Co., Ltd；Choosing
The 50% dimethomorph wettable powder taken derives from BASF European Co., Ltd.
(4) cell arrangement
Plot area is 1.5m × 8m, and 8 vines, random district's groups arrangement are planted per cell.
3 reagent agent experimental design of table
| Processing number | Medicament | Extension rate |
| 1 | (proportioning is 8.89 to biogas slurry pyraclostrobin composite bactericide:1) | 500:4444.4 |
| 2 | (proportioning is 10.0 to biogas slurry pyraclostrobin composite bactericide:1) | 500:5000 |
| 3 | (proportioning is 14.81 to biogas slurry pyraclostrobin composite bactericide:1) | 300:4444.4 |
| 4 | (proportioning is 16.67 to biogas slurry pyraclostrobin composite bactericide:1) | 300:5000 |
| 5 | 250 grams per liter pyraclostrobins | 4000 |
| 6 | 0.33% biogas slurry | 300 |
| 7 | Blank control | —— |
4 reagent agent experimental design of table
| Processing number | Medicament | Extension rate |
| 1 | (proportioning is 4.44 to biogas slurry dimethomorph composite bactericide:1) | 500:2222.2 |
| 2 | (proportioning is 5.0 to biogas slurry dimethomorph composite bactericide:1) | 500:2500 |
| 3 | (proportioning is 7.41 to biogas slurry dimethomorph composite bactericide:1) | 300:2222.2 |
| 4 | (proportioning is 8.33 to biogas slurry dimethomorph composite bactericide:1) | 300:2500 |
| 5 | 50% dimethomorph | 2000 |
| 6 | 0.33% biogas slurry | 300 |
| 7 | Blank control | —— |
2.2 application method
2.2.1 application method
It is sprayed with workers and peasants' l6 sprayers, is compared with clear water.Medication for the first time has no morbidity, continuous use 3 times, interval
7-10d, 10d carries out field investigation after 8d and the 3rd medicine after the 2nd medicine.
2.2.2 the medicament data of other pest and disease damages is prevented
During experiment, each cell does not spray other any fungicide in addition to spraying test medicament.
2.3 investigation methods and statistical analysis
10 new branches are randomly selected per cell, investigate whole blades on each branch, the sick number of sheets, sick series is investigated, presses
Scab accounts for the percentage classification record of entire leaf area.Disease index is calculated, and finds out preventive effect.
2.4 drug effect computational methods
a、
b、
Multiple range test is carried out to each preventive effect using the Duncan duncan's new multiple range methods of DPSv12.01 data processing softwares.
2.5 test result
In this embodiment, component I biogas slurries are chosen and dilute 300-500 times, component II dilutes 2000-5000 times and is compounded,
Field trial is carried out for below choosing, component I biogas slurries and the weight proportion of component II pyraclostrobins are selected 8.89：1-
Value between 16.67, wherein 300 times of 4000 times of 250 grams per liter pyraclostrobin missible oil, biogas slurry compoundings；Component I biogas slurries and component
The weight proportion of II dimethomorphs is 4.44：1-8.33：Value between 1, with 2000 times of 50% dimethomorph wettable powder, natural pond
300 times of compoundings of liquid.
5 biogas slurry of table and the 250 mixed field efficacies to downy mildew of garpe of grams per liter pyraclostrobin
Note：Data are to manage average data everywhere in table
Biogas slurry and pyraclostrobin 8.89：1、10.0：1、14.81：1、16.67：1 mixed shows good diseases prevention effect
Fruit, after secondary medicine 10 days after 8 days and last medicine, biogas slurry and pyraclostrobin 8.89：1、14.81：1 liang of processing is to grape downy mildew
The preventive effect of disease is significantly better than 4000 times of 250 grams per liter pyraclostrobin；Biogas slurry and pyraclostrobin 10.0：1、16.67：At 1 liang
It manages suitable with 4000 times of 250 grams per liter pyraclostrobin to the control effect of downy mildew of garpe.Biogas slurry and pyraclostrobin compounding group
It closes object and biogas slurry single dose is significantly better than to the control effect of downy mildew of garpe.During pilot survey, each chemicals treatment is to supplying
Try grape variety safety, no poisoning.
6 biogas slurry of the table field efficacy to downy mildew of garpe mixed with 50% dimethomorph
Biogas slurry and 50% dimethomorph 4.44：1、5.0：1、7.41：1、8.33：1 mixed shows good diseases prevention effect
Fruit, after secondary medicine 10 days after 8 days and last medicine, biogas slurry and dimethomorph 4.44：1、7.41：1 liang of processing is to downy mildew of garpe
Preventive effect be significantly better than 2000 times of 50% dimethomorph；Biogas slurry and dimethomorph 5.0：1、8.33：1 liang of processing is to grape downy mildew
The control effect of disease is suitable with 2000 times of 50% dimethomorph.Biogas slurry and dimethomorph complex composition prevent downy mildew of garpe
It controls effect and is significantly better than biogas slurry single dose.During pilot survey, each chemicals treatment is to for examination grape variety safety, no medicine
Evil.
Embodiment 3
Safety testing
The content 10%~20% that agent content is less than commercial product is sterilized in complex composition, biogas slurry single dose is pollution-free, nothing
Residual, therefore toxicity of the complex composition to people and animals is satisfactory with the safety to crop.Composite bactericide is through recognizing
Mechanism toxicity test is determined the result shows that belonging to micro-virus kind pesticide：
Rat acute toxicity LD50 ♂>5000mg/kg, ♀>5000mg/kg；
Rat acute percutaneous toxicity LD50 ♂>5000mg/kg, ♀>5000mg/kg；
Lagophthalmos stimulation integral is 0 (1h), is 0 (1 after 48h：100) it dilutes, it is nonirritant to eye；
Lagophthalmos stimulation integral is 0 (4h), to no skin irritation.
The present invention is screened by a large amount of bioassay, finds biogas slurry and carboxylic acid amide series bactericidal agent, methoxy acrylic acid
Esters fungicide, phenyl amide series bactericidal agent, carbamate fungicide, cyanoacetamide series bactericidal agent, the sterilization of organic sulfur class
Agent, a kind of of phenylimidazole series bactericidal agent are compounded with certain proportion, effectively prevent downy mildew of garpe.The present invention is to containing biogas slurry and killing
Application of the complex composition of microbial inoculum in prevention downy mildew of garpe, carries out verifying its effect by a large amount of indoor and field trials
Fruit.Active ingredient of the present invention includes components I and compositionⅱ, and the weight of formulation proportioning of components I and compositionⅱ is 100:1-1：50 or
50：1-1：50 or 20：1-1：20 or 15：1-1：10 or 10：1-1：5 or 5：1-1：The value of the value present invention is carried out between 1 not
It is limited to range above, because enumerating the limitation of numerical value, is illustrated according to the above section, but for falling 100：1-1：50 ranges
Interior value, all belongs to the scope of protection of the present invention.The present invention is chosen by choosing component I for chicken manure biogas slurry, component II respectively
For pyraclostrobin single dose, dimethomorph single dose, value is respectively carried out in the above weight proportion section, is measured for grape
The bacteriostasis rate of downy mildew.
In multiple value ranges of components I provided by the invention and compositionⅱ weight proportion, proportioning is more preferably
20:1-1:1, when wherein compositionⅱ selects pyraclostrobin, synergy proportioning is 20:1-5:1, further filter out 14.81:1 is
Optimal proportion.In another preferred technical solution, the preparation proportioning preferably 10 of the components I and compositionⅱ:1-1:1, into
One step filters out 7.41:1 is optimal proportion, and the compositionⅱ is dimethomorph, the synergistic effect between components I and compositionⅱ
Significantly.
In conclusion the present invention filters out biogas slurry and fungicide pyrazoles ether bacterium by Toxicity Determination and field trial
The optimal proportion of ester, dimethomorph, which has synergistic function, compared with existing single formulation, sterilization
Raising with obvious effects, and to crop safety, be worth promoting and applying in agricultural production, reduce Pesticide environment pollution, control to reach
The purpose of pathogenic bacteria drug resistance occurrence and development processed.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment includes
One independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should incite somebody to action
As a whole, the technical solutions in the various embodiments may also be suitably combined for specification, and forming those skilled in the art can
With the other embodiment of understanding.
Claims (10)
1. a kind of bactericide compounded composition of biogas slurry-, it is characterised in that：Active ingredient includes components I and compositionⅱ, the component
I is biogas slurry, the compositionⅱ be carboxylic acid amide series bactericidal agent, methoxy acrylic bactericide, phenyl amide series bactericidal agent,
Carbamate fungicide, cyanoacetamide series bactericidal agent, organic sulfurcontaining fungicide, phenylimidazole series bactericidal agent it is any.
2. a kind of bactericide compounded composition of biogas slurry-as described in claim 1, it is characterised in that：The components I and compositionⅱ
Weight proportion be 100:1-1:50.
3. a kind of bactericide compounded composition of biogas slurry-as described in claim 1, it is characterised in that：The components I and compositionⅱ
Weight proportion be 20:1-5:1.
4. a kind of bactericide compounded composition of biogas slurry-as described in claim 1, it is characterised in that：The components I and compositionⅱ
Weight proportion be 10:1-1:1.
5. the bactericide compounded composition of a kind of biogas slurry-according to any one of claims 1-4, it is characterised in that：The carboxylic acid
Acid amide fungicides be dimethomorph, flumorph, iprovalicarb, mandipropamid it is any.
6. the bactericide compounded composition of a kind of biogas slurry-according to any one of claims 1-4, it is characterised in that：The methoxy
Base acrylic bactericide be ZEN 90160, pyrazoles ether bacterium, kresoxim-methyl, trifloxystrobin it is any.
7. the bactericide compounded composition of a kind of biogas slurry-according to any one of claims 1-4, it is characterised in that：It is described organic
Sulfurcontaining fungicide be Mancozeb, Propineb, Carbatene it is any.
8. the bactericide compounded composition of a kind of biogas slurry-according to any one of claims 1-4, it is characterised in that：The phenyl
Acid amide fungicides are metalaxyl；
Or the carbamate fungicide is Propamocarb；
Or the cyanoacetamide series bactericidal agent is cymoxanil；
Or the phenylimidazole class protective fungicide is cyazofamid.
9. a kind of application of the bactericide compounded composition of biogas slurry-, it is characterised in that：
Using the bactericide compounded composition of any one of the claim 1-4 biogas slurries-, component I dilutes 300-500 times, 0.5-
50% component II dilutes 2000-5000 times and applies in the prevention of crop disease.
10. a kind of application of the bactericide compounded composition of biogas slurry-as claimed in claim 9, it is characterised in that：The crop is
One or more of grape, melon vegetables, brassicaceous vegetable, cucurbitaceae vegetable, leguminous vegetable, the disease are downy mildew
Disease.
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| CN108184903A (en) * | 2018-02-10 | 2018-06-22 | 山东省烟台市农业科学研究院 | A kind of chicken manure biogas slurry and bactericide compounded composition and the application in apple leaf diseases are prevented |
| CN108576056A (en) * | 2018-02-10 | 2018-09-28 | 山东省烟台市农业科学研究院 | A kind of complex composition of biogas slurry containing chicken manure-insecticide and its application |
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| CN108184903A (en) * | 2018-02-10 | 2018-06-22 | 山东省烟台市农业科学研究院 | A kind of chicken manure biogas slurry and bactericide compounded composition and the application in apple leaf diseases are prevented |
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|---|---|---|---|---|
| CN106518364A (en) * | 2016-10-28 | 2017-03-22 | 成都鑫瑞现代农业开发有限公司 | Chrysanthemum leaf fertilizer |
| CN108184903A (en) * | 2018-02-10 | 2018-06-22 | 山东省烟台市农业科学研究院 | A kind of chicken manure biogas slurry and bactericide compounded composition and the application in apple leaf diseases are prevented |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184903A (en) * | 2018-02-10 | 2018-06-22 | 山东省烟台市农业科学研究院 | A kind of chicken manure biogas slurry and bactericide compounded composition and the application in apple leaf diseases are prevented |
| CN108576056A (en) * | 2018-02-10 | 2018-09-28 | 山东省烟台市农业科学研究院 | A kind of complex composition of biogas slurry containing chicken manure-insecticide and its application |
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