CN101438703A - Sterilizing compositional composition containing eugenol - Google Patents
Sterilizing compositional composition containing eugenol Download PDFInfo
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- CN101438703A CN101438703A CNA2008102385272A CN200810238527A CN101438703A CN 101438703 A CN101438703 A CN 101438703A CN A2008102385272 A CNA2008102385272 A CN A2008102385272A CN 200810238527 A CN200810238527 A CN 200810238527A CN 101438703 A CN101438703 A CN 101438703A
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- eugenol
- fludioxonil
- compound composition
- antifungal compound
- composition containing
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Abstract
The invention discloses a bactericide for controlling and curing the plant fungal diseases, and particularly discloses an antifungal compound composition containing eugenol. The antifungal compound composition contained with the eugenol is characterized in that eugenol and fludioxonil are included, and the weight ratio of active ingredients of the eugenol and the fludioxonil ranges from 9:1 to 1:9. The antifungal compound composition contained with the eugenol is further improved, i.e., the weight ratio of the active ingredients of the eugenol and the fludioxonil is 1:1. Since the fludioxonil is combined with the eugenol, the antifungal compound composition has special effect for controlling the fungal diseases of the crops, displays the synergic action and the sterilization effect and well controls the fungal diseases on the crops or the picked fruits and vegetables.
Description
(1), technical field
The present invention relates to the bactericide of a kind of control and treatment fungal diseases of plants, particularly a kind of compound sterilization composition that contains eugenol.
(2), background technology
It is by fungus-caused that 80% plant disease is arranged approximately.Plant disease not only reduces the yield and quality of crop, and is bigger by fungus-caused harm and loss in the fruit vegetables storing transportation.Fludioxonil derives from the natural products pyrrolnitrin, new pyrrole class, non-internal-absorting, wide-spectrum bactericide.The disease that Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium bacterium etc. are caused has good protection and therapeutic action.Be used for the rotten sickle spore bacterium of control snow, wheat black rot, rhizoctonia solani etc., gray mold is had special efficacy.Eugenol, chemical name are 2-methoxyl group-4-chavicol.Colourless to weak yellow liquid, turn brown aloft, be present in caryophyllus oil, cinnamon leaves oil, interior cassia oil, camphorated oil, the nutmeg wet goods.Eugenol mainly shows as antibacterial, anesthesia, analgesic, anti-oxidant, antitumor etc. at medically pharmacologically active.Eugenol has the strong aroma of spice, is commonly used for toothpaste spices, antioxidant and preservative in the life.Eugenol also has application on agricultural, Li Peng's rosy clouds report that this compound has inhibitory action indoor to apple storage period pathogenic bacteria such as apple mould, grey mold and anthrax etc.
(3), summary of the invention
In order to remedy the deficiencies in the prior art, the invention provides a kind of compound sterilization composition that contains eugenol of efficient, low toxicity, said composition can be used on the crop or adopts the control or the treatment of back fruits and vegetables fungal disease.
The present invention is achieved through the following technical solutions:
A kind of compound sterilization composition that contains eugenol, its special character is: it contains eugenol and fludioxonil, and the two active ingredient weight proportion of eugenol and fludioxonil is 9:1～1:9.
The compound sterilization composition that contains eugenol of the present invention, it further improves and is: the two active ingredient weight proportion of eugenol and fludioxonil is 1:1.
The compound sterilization composition that contains eugenol of the present invention, the percentage by weight that described eugenol and fludioxonil active ingredient account for whole use solvent is: 0.1-0.4%.
Described bactericide can be prepared according to a conventional method by eugenol, fludioxonil solvent and auxiliary agent; " water mixing can be used " form that also can be independent is by the mixture that the independent preparation of single active component is made, for example " bucket mixes ".
Fludioxonil of the present invention has special efficacy in conjunction with eugenol to the fungal disease of control crop, and demonstrates the Synergistic sterilizing effect, on the crop or adopt back fruits and vegetables fungal disease the better controlled effect is all arranged.Specifically have the following advantages:
1, synergy, result of the test show that eugenol all has obvious synergistic effect by different proportion is composite to different phytopathogens with fludioxonil.
2, toxicity is low, and eugenol and fludioxonil toxicity are all very low, to human and environment safety.Eugenol is to rat acute per os LD
503000mg/kg; Fludioxonil is to rat acute per os LD
50Male or female〉5000mg/kg.
(4), embodiment:
Embodiment 1: the composite indoor prescription screening of eugenol and fludioxonil
One, reagent agent:
A:99% eugenol (the permanent really spices refinery of Jiangxi Province's Ji'an City)
The former medicine of B:95.8% fludioxonil (Switzerland is just reaching earlier crop protection Co., Ltd)
Below be the weight proportion of the two active ingredient:
AB1: eugenol: fludioxonil=9:1
AB2: eugenol: fludioxonil=8:2
AB3: eugenol: fludioxonil=7:3
AB4: eugenol: fludioxonil=6:4
AB5: eugenol: fludioxonil=5:5
AB6: eugenol: fludioxonil=6:4
AB7: eugenol: fludioxonil=3:7
AB8: eugenol: fludioxonil=2:8
AB9: eugenol: fludioxonil=1:9
Two, for the examination germ
Graw mold of tomato (Botrytis cinerea).
Three, test method
Adopt the mycelial growth rate determination method: establish 13 processing, it is eugenol, fludioxonil, the different proportionings of 9 eugenols and fludioxonil, solvent control and blank, join two kinds of medicaments in the PDA medium of quantitative fusing by different proportion, make the ultimate density of two single agent and composite dose be respectively 100,50,25,12.5,6.25mg/L, fully shake up, pour in the sterilized culture dish, after treating culture medium solidifying, produce the bacterium dish with diameter for the 0.5cm card punch, with transfer needle the bacterium dish is inoculated into medium central authorities, mycelia faces down, one in every ware, not handle repeat 3 times, measure colony diameter with the right-angled intersection method after placing 25～26 ℃ of incubators to cultivate 48h.Mean value calculation bacteriostasis rate with 3 repetitions.Computing formula is:
Calculate inhibiting rate according to formula, utilize the percentage and the probability value table of comparisons that inhibiting rate is converted into the probability value, and dose concentration is converted into logarithm, obtain virulence formula y=a+bx with dosage logarithm value (x) and probability value (y), calculate correlation coefficient r and suppress in concentration EC
50
The interaction of calculating mixed agent according to the method for Wadley, with the coefficient of synergism SR value analysis effect that is mixed:
EC
50 (theoretical values)=(a+b)/[a/EC
50 (A)+ b/EC
50 (B)];
SR=EC
50 (theoretical values)/ EC
50 (real values)
A, B represent two kinds of components in the mixture respectively;
A, b represent the ratio of two kinds of single agent in mixture respectively;
EC
50 (real values)Practical measurement value for specific mixture;
1.5 represent to have synergistic effect after two kinds of single agent are mixed;
Has antagonism after SR≤two kinds of single agent of 0.5 expression are mixed;
0.5 have summation action after≤SR≤two kinds of single agent of 1.5 expressions are mixed.
Four, result of the test
Two kinds of single agent of eugenol and fludioxonil and different complex composition are to inhibiting rate, virulence regression equation, correlation coefficient r, the concentration EC in effective inhibition of test plant pathogen
50See Table 1 with the coefficient of synergism SR of mixture.
Annotate: each ratio of eugenol and fludioxonil is the ratio of active ingredient in the table.
Found out that by table 1 composition AB5 activity is the highest, coefficient of synergism is 1.77.
Embodiment 2: eugenol fludioxonil composite bactericide live test
One, for the examination material
Citrus and garlic stems
Two, reagent agent
The composition of 20% eugenol fludioxonil composite bactericide: eugenol: fludioxonil=1:1
Three, for the examination bacterium
Oranges and tangerines penicilliosis (Penicillium italicum Wehmer), garlic stems gray mold (Botrytis cinerea)
Four, test method
1, reagent preparation
20% eugenol fludioxonil composite bactericide is diluted 100 times, 200 times and 500 times respectively.
2, to the effectiveness of scutellum rot of orange
With the wound of transfer needle at fruit metastomium thorn 3mm (wide) * 4mm (deeply), connect mass concentration respectively for diluting 20% eugenol fludioxonil composite bactericide, the 60 μ L of 100 times, 200 times and 500 times, leave standstill 2h after, inoculate 5 * 10 respectively
5Germ spore suspension 20 μ L.Treat after wound dries fruit to be placed the plastic packing box of 300mm * 200mm * 100mm, the overcoat plastic sack to be to keep relative moisture about 95%, deposits in the room temperature of (25 ± 1) ℃.Contrast is for only inoculating the fruit of germ.20 fruits of every processing repeat 3 times.Measure fruit scab diameter (diameter=scab diameter-0.3cm) and rotten index after 5 days.
The situation of rotting is divided into 5 grades by weight:
0 grade, do not rot;
1 grade, rotten area accounts for the fruit area below 25%;
2 grades, rotten area accounts for fruit area 25%～50%;
3 grades, rotten area accounts for fruit area 50%～75%;
4 grades, rotten area accounts for fruit area 75%～100%.The computing formula of rotten index is as follows:
3, the inhibitory action that garlic stems is rotted
Earlier garlic stems being soaked and immersing concentration respectively is 1 * 10
7About 1min in the Botrytis cinerea bacteria suspension of cfu/mL is soaked in 1min in the 20% eugenol fludioxonil composite bactericide of dilution 100 times, 200 times and 500 times respectively after drying, taking-up is dried, and the common polythene bag of packing into is in normal temperature storage down.The statistics incidence of disease and rotten index behind the 7d.Contrast is for only inoculating the garlic stems of the rotten grape spore of Botrytis cinerea and green onion, and each processing is 20 garlic stems, establishes three repetitions.
0 grade: no mildew;
1 grade: the length of mildew part is less than 25% a kind of sedge tip length;
2 grades: the length of mildew part accounts for 25%～50% of total a kind of sedge tip length;
3 grades: the length of mildew part accounts for 50%～75% of a kind of sedge tip length;
4 grades, the length of mildew part accounts for 75%～100% of total a kind of sedge tip length.
Five, result of the test
The inhibitory action of table 2 pair scutellum rot of orange
The inhibitory action of table 3 pair garlic stems gray mold
Claims (3)
1, a kind of compound sterilization composition that contains eugenol, it is characterized in that: it contains eugenol and fludioxonil, and the two active ingredient weight proportion of eugenol and fludioxonil is 9: 1～1: 9.
2, the compound sterilization composition that contains eugenol according to claim 1 is characterized in that: the two active ingredient weight proportion of eugenol and fludioxonil is 1: 1.
3, the compound sterilization composition that contains eugenol according to claim 1 is characterized in that: the percentage by weight that eugenol and fludioxonil active ingredient account for whole use solvent is: 0.1-0.4%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102385272A CN101438703B (en) | 2008-12-18 | 2008-12-18 | Sterilizing compositional composition containing eugenol |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102385272A CN101438703B (en) | 2008-12-18 | 2008-12-18 | Sterilizing compositional composition containing eugenol |
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| Publication Number | Publication Date |
|---|---|
| CN101438703A true CN101438703A (en) | 2009-05-27 |
| CN101438703B CN101438703B (en) | 2011-10-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102385272A Active CN101438703B (en) | 2008-12-18 | 2008-12-18 | Sterilizing compositional composition containing eugenol |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688936A (en) * | 2013-08-15 | 2014-04-02 | 山东省农业科学院植物保护研究所 | Compound bactericide containing eugenol and cyazofamid and application thereof |
| CN104430358A (en) * | 2013-09-17 | 2015-03-25 | 南京农业大学 | composition containing fludioxonil and kresoxim-methyl |
| CN104430364A (en) * | 2013-09-18 | 2015-03-25 | 南京农业大学 | Composition containing fludioxonil and azoxystrobin |
| CN104886055A (en) * | 2015-06-27 | 2015-09-09 | 响水县智诚知识产权信息服务有限公司 | Plant growth regulator |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0418047D0 (en) * | 2004-08-12 | 2004-09-15 | Syngenta Participations Ag | Fungicidal compositions |
-
2008
- 2008-12-18 CN CN2008102385272A patent/CN101438703B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688936A (en) * | 2013-08-15 | 2014-04-02 | 山东省农业科学院植物保护研究所 | Compound bactericide containing eugenol and cyazofamid and application thereof |
| CN103688936B (en) * | 2013-08-15 | 2016-02-03 | 山东省农业科学院植物保护研究所 | A kind of composite bactericide and application thereof containing eugenol and cyazofamid |
| CN104430358A (en) * | 2013-09-17 | 2015-03-25 | 南京农业大学 | composition containing fludioxonil and kresoxim-methyl |
| CN104430364A (en) * | 2013-09-18 | 2015-03-25 | 南京农业大学 | Composition containing fludioxonil and azoxystrobin |
| CN104886055A (en) * | 2015-06-27 | 2015-09-09 | 响水县智诚知识产权信息服务有限公司 | Plant growth regulator |
| CN104886055B (en) * | 2015-06-27 | 2017-05-24 | 王东强 | Plant growth regulator |
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| Publication number | Publication date |
|---|---|
| CN101438703B (en) | 2011-10-12 |
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Effective date of registration: 20230921 Address after: No. 87 Fuyang Street, Jibei Street, Jiyang District, Jinan City, Shandong Province, 251400 (600 meters west of the bus station and north of the road) Patentee after: SHANDONG AOWEITE BIOTECHNOLOGY Co.,Ltd. Address before: The Jiyang County of Ji'nan City, Shandong Province Economic Development Zone Fuyang 251400 Street northbound Patentee before: SHANDONG NUTRIENT SOURCE FOOD TECHNOLOGY Co.,Ltd. |