CN103637953B - Torch tree fruit extract and preparation method thereof and preparing the purposes in cosmetics - Google Patents
Torch tree fruit extract and preparation method thereof and preparing the purposes in cosmetics Download PDFInfo
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Abstract
The invention provides a kind of preparation method of torch tree fruit extract, the method comprises: (1) is dry and pulverize by torch tree fruit, obtains torch tree fruit dry powder; (2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, (3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid; (4) ethanol in the distillation removing described stillness of night, obtain distilling residue, then will distill residue to mix with water, obtain material to be extracted, and material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, n-butyl alcohol extract removing n-butyl alcohol will be obtained to obtain torch tree fruit extract.Present invention also offers torch tree fruit extract that preparation method as above obtains and preparing the purposes in cosmetics.By technique scheme, torch tree fruit extract of the present invention has outstanding antioxidant activity.
Description
Technical field
The present invention relates to agricultural biological technical field, particularly, relate to a kind of preparation method of torch tree fruit extract, torch tree fruit extract that this preparation method obtains and preparing the purposes in cosmetics.
Background technology
Torch tree (Rhustyphina), has another name called Rhus typhina, is Anacardiaceae Rhus defoliation small arbor.Fruit ear cerise, oblate spheroid, has red seta, and tight consor is torch-like.Because its fruit is red gorgeous, tree-like as torch round and round, be called as " torch tree ".Torch tree originates in North America, is suitable for growing on open sandy soil or calculous soil.China is introduced a fine variety by Institute of Botany, Chinese Academy of Sciences nineteen fifty-nine, more in the cultivation of northern each province, is mainly used in green barren hill and doubles as saline-alkali wasteland scenic forest seeds.Torch tree leaves is in great numbers, surperficial villous, and the floating dust in a large amount of adsorb atmospheric of energy and harmful substance, cattle and sheep are Shiqi blade not, does not endanger by sick worm.Torch tree is not only drought-resistant, saline and alkaline, barren, very easily breeds, and have higher to view and admire, practical value.
Torch tree cultivated area is comparatively large, and therefore output is also larger.Further, antioxidant content content comparatively horn of plenty in the fruit of torch tree.But the antioxidation Rate activity extracting the extract obtained in the fruit of torch tree is lower, be difficult to meet the requirement preparing cosmetics.
Summary of the invention
The present inventor finds, torch tree fruit ethanol water is extracted under Ultrasonic Conditions and is extracted with ethyl acetate, the extract with higher α glucosidase inhibitory active can be obtained, and, remaining for extraction into ethyl acetate aqueous phase is used n-butanol extraction again, just can obtain the torch tree fruit extract that antioxidation Rate activity significantly improves, solve extraction simultaneously from torch tree fruit thus to there is the extract of higher α glucosidase inhibitory active and there is the technical problem of the extract compared with high anti-oxidation Rate activity, obtain the present invention.
The invention provides a kind of preparation method of torch tree fruit extract, the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtain distilling residue, then will distill residue to mix with water, obtain material to be extracted, and material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, n-butyl alcohol extract removing n-butyl alcohol will be obtained to obtain torch tree fruit extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10-30L, and extracting ethyl acetate used is 5-10L, and extracting n-butyl alcohol used is 5-10L.
Present invention also offers the torch tree fruit extract that preparation method as above prepares.
Present invention also offers torch tree fruit extract described above and prepare the purposes in cosmetics.
By technique scheme, the preparation method of torch tree fruit extract of the present invention can extract the extract with higher α glucosidase inhibitory active and the extract had compared with high anti-oxidation Rate activity from torch tree fruit simultaneously.
Other features and advantages of the present invention are described in detail in detailed description of the invention part subsequently.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail.Should be understood that, detailed description of the invention described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
In the present invention, when not doing contrary explanation, the liquid of use or the volumetric quantities of gas are all 20 DEG C, the numerical value of 1 standard atmosphere pressure.
The invention provides a kind of preparation method of torch tree fruit extract, the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtain distilling residue, then will distill residue to mix with water, obtain material to be extracted, and material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, n-butyl alcohol extract removing n-butyl alcohol will be obtained to obtain torch tree fruit extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10-30L, and extracting ethyl acetate used is 5-10L, and extracting n-butyl alcohol used is 5-10L.
Wherein, in step (1), dry condition can be the drying condition that fruit drying is commonly used, and such as, dry condition can make the water content of torch tree fruit dry powder be 5-10 % by weight; Such as, dry temperature can be 40-70 DEG C.
Wherein, in step (1), the condition of pulverizing can be the conventional drying condition of fruit drying, and preferably, the condition of pulverizing makes torch tree fruit dry powder can by 60 mesh sieves.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %.
Wherein, in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation can be 20-100W, the time of Ultrasonic Radiation can be 30-60 minute, and the frequency of Ultrasonic Radiation can be 25-55kHz, and the temperature of Ultrasonic Radiation can be 20-35 DEG C.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C.
Wherein, in step (4), the temperature of the ethanol in the distillation removing described stillness of night can be 40-50 DEG C.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L, and extracting n-butyl alcohol used is 7-8L.
Wherein, as the particularly preferred a kind of embodiment of the present invention, in step (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L, and extracting n-butyl alcohol used is 7-8L.In this preferred implementation, further can improve the productive rate of torch tree fruit extract and the antioxidation Rate activity of torch tree fruit extract.
Present invention also offers the torch tree fruit extract that preparation method as above prepares.
Present invention also offers torch tree fruit extract described above and prepare the purposes in cosmetics.
Wherein, preferably, described cosmetics are eye cream.Typically, can by torch tree fruit extract and caprylyl glycol, beta glucan, polydimethylsiloxane, hyaluronate sodium, glycerol, EHA, butanediol and water mixing, relative to the torch tree fruit extract of 1 weight portion, the consumption of caprylyl glycol is 0.1-0.5 weight portion, the consumption of beta glucan is 0.5-2 weight portion, the consumption of polydimethylsiloxane is 3-15 weight portion, the consumption of hyaluronate sodium is 0.01-0.05 weight portion, the consumption of glycerol is 1-5 weight portion, the consumption of EHA is 0.5-2 weight portion, the consumption of butanediol is 1-5 weight portion, the consumption of water is 30-50 weight portion.
Describe the present invention by the following examples.
Embodiment 1
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 20L.And material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 7.5L, and extracting n-butyl alcohol used is 7.5L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 2
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 65 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 40W, the time of ultrasonic Treatment is 40 minutes, the frequency of ultrasonic Treatment is 35kHz, the temperature of ultrasonic Treatment is 25 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15L.And material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 7L, and extracting n-butyl alcohol used is 7L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 3
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 75 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 15L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 60W, the time of ultrasonic Treatment is 50 minutes, the frequency of ultrasonic Treatment is 45kHz, the temperature of ultrasonic Treatment is 30 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
Residue will be distilled mix with water, obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 25L and material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 8L, and extracting n-butyl alcohol used is 8L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 4
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 50 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 20L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 100W, the time of ultrasonic Treatment is 60 minutes, the frequency of ultrasonic Treatment is 55kHz, the temperature of ultrasonic Treatment is 20 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 30L.And material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 10L, and extracting n-butyl alcohol used is 10L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 5
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 90 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 20W, the time of ultrasonic Treatment is 30 minutes, the frequency of ultrasonic Treatment is 25kHz, the temperature of ultrasonic Treatment is 35 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10L.And material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 5L, and extracting n-butyl alcohol used is 5L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.。
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Comparative example 1
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 100L.And material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 3L, and extracting n-butyl alcohol used is 3L.The organic facies obtained by extraction into ethyl acetate is as acetic acid ethyl ester extract.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that this comparative example obtains.
Comparative example 2
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 20L.And material to be extracted is carried out n-butanol extraction, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting n-butyl alcohol used is 7.5L.The organic facies obtained by n-butanol extraction as n-butyl alcohol extract, and is distilled at 45 DEG C, the levels of n-butanol in n-butyl alcohol extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that this comparative example obtains.
Comparative example 3
The method identical with embodiment 1 is adopted to prepare torch tree fruit extract, unlike, n-butyl alcohol is changed to chloroform.
Comparative example 4
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
Then be less than 1 % by weight by the lyophilization of distillation residue to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that this comparative example obtains.
Testing example 1
According to document (Stephanie Dudonne et al.; Comparative Study of AntioxidantProperties and Total Phenolic Content of30Plant Extracts of Industrial InterestUsing DPPH; ABTS; FRAP; SOD, and ORAC Assays; J.Agric.Food Chem., 2009,57 (5)) method in measures iron ion oxidation resistance and the oxyradical absorbability of the torch tree fruit extract of embodiment 1-5 and comparative example 1-4, and result is as shown in table 1.Also list relative to the fresh torch tree fruit of 1kg in table 1, the output of torch tree fruit extract.
Table 1
As can be seen from Table 1, the preparation method of torch tree fruit extract of the present invention can also obtain the torch tree fruit extract side-product with higher antioxidation Rate activity.Further, in preferred steps (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, extracting ethyl acetate used is 7-8L, when to extract n-butyl alcohol used be 7-8L, further can improve productive rate and the antioxidation Rate activity of torch tree fruit extract.
Testing example 2
Acetic acid ethyl ester extract embodiment 1-5 and comparative example 1 and 3 obtained distills respectively at 45 DEG C, the ethyl acetate content in acetic acid ethyl ester extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract side-product that embodiment 1-5 and comparative example 1 and 3 obtain respectively.
According to document (Yao, Y.; Sang, W.; Zhou, M.J.; Ren, G.X.Antioxidantand α-Glucosidase inhibitory activity of colored grains in China.Journal ofAgricultural and Food Chemistry.2010, method 58:770-774), the IC of the torch tree fruit extract side-product Inhibiting α-glucosidase activity that embodiment 1-5 and comparative example 1 and 3 obtain
50value, result is as shown in table 1.The output of the antioxidant content product that the torch tree fruit dry powder calculating every 1kg in the method for embodiment 1-5 and comparative example 1 and 3 can extract, result is as shown in table 2.
Table 2
As can be seen from Table 2, the preparation method of torch tree fruit extract of the present invention can also obtain the torch tree fruit extract side-product with higher α glucosidase inhibitory active.Further, in preferred steps (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, extracting ethyl acetate used is 7-8L, when to extract n-butyl alcohol used be 7-8L, further can improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract side-product.
Testing example 3
The torch tree fruit extract that embodiment 1 and comparative example 2 and 4 obtain is mixed with caprylyl glycol, beta glucan, polydimethylsiloxane, hyaluronate sodium, glycerol, EHA, butanediol and water respectively, obtains eye cream.Relative to the torch tree fruit extract of 1 weight portion, the consumption of caprylyl glycol is 0.3 weight portion, the consumption of beta glucan is 1 weight portion, the consumption of polydimethylsiloxane is 6 weight portions, the consumption of hyaluronate sodium is 0.03 weight portion, and the consumption of glycerol is 2 weight portions, and the consumption of EHA is 1 weight portion, the consumption of butanediol is 2 weight portions, and the consumption of water is 50 weight portions.
Choose the experimenter that 60 routine periocualr skin pigmentations are many, age is between 30-50 year, sooner or later after clean skin, as above obtained eye cream is applied in skin around the eyes equably, sooner or later respectively once, continuous use finds that eye cream prepared by the torch tree fruit extract using embodiment 1 to obtain can make the pigmentation of the skin around the eyes of the experimenter of more than 90% make moderate progress for 30 days afterwards, and eye cream prepared by the torch tree fruit extract using comparative example 2 and 4 to obtain only can make the pigmentation of the skin around the eyes of the experimenter of about 20% make moderate progress.
As can be seen here, torch tree fruit extract of the present invention can be used in preparing cosmetics (particularly eye cream) and can obtaining improving Pigmented effect better.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned detailed description of the invention, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible compound mode.
In addition, also can carry out combination in any between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (9)
1. a preparation method for torch tree fruit extract, is characterized in that: the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtain distilling residue, then will distill residue to mix with water, obtain material to be extracted, and material to be extracted is carried out extraction into ethyl acetate and n-butanol extraction successively, n-butyl alcohol extract removing n-butyl alcohol will be obtained to obtain torch tree fruit extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L, and extracting n-butyl alcohol used is 7-8L.
2. preparation method according to claim 1, is characterized in that: in step (1), and dry condition makes the water content of torch tree fruit dry powder be 5-10 % by weight, and the condition of pulverizing makes torch tree fruit dry powder can by 60 mesh sieves.
3. preparation method according to claim 1, is characterized in that: in step (2), and relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %.
4. preparation method according to claim 1, it is characterized in that: in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 20-100W, the time of Ultrasonic Radiation is 30-60 minute, the frequency of Ultrasonic Radiation is 25-55kHz, and the temperature of Ultrasonic Radiation is 20-35 DEG C.
5. preparation method according to claim 4, it is characterized in that: in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, the time of Ultrasonic Radiation is 40-50 minute, the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C.
6. preparation method according to claim 1, is characterized in that: in step (2), and relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L, and extracting n-butyl alcohol used is 7-8L.
7. the torch tree fruit extract that the preparation method in claim 1-6 described in any one prepares.
8. torch tree fruit extract according to claim 7 is preparing the purposes in cosmetics.
9. purposes according to claim 8, is characterized in that: described cosmetics are eye cream.
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| CN107349241B (en) * | 2017-08-03 | 2020-11-20 | 吉林大学 | Application of torch tree fruit extract in preparation of anti-aging medicine |
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