CN104706695A - Rhus hirta fruit extract product preparation method - Google Patents
Rhus hirta fruit extract product preparation method Download PDFInfo
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Abstract
The invention provides a rhus hirta fruit extract product preparation method which comprises the following steps: (1) drying and crushing rhus hirta fruit to obtain dry rhus hirta fruit powder; (2) mixing the dry rhus hirta fruit powder with an extracting solution to obtain a mixed material; (3) processing the mixed material with ultrasonic radiation, removing solids in the ultrasonic radiation treated material to obtain supernatant; (4) removing ethanol in the supernatant by distilling to obtain distilled residue, mixing the distilled residue with water to obtain a to-be-extracted material, extracting with ethyl acetate to obtain an extract, and removing the ethyl acetate in the extract. The rhus hirta fruit extract product prepared by the method has high alpha glucosidase inhibitory activity, and the alpha glucosidase inhibition activity IC50 value is below 2500mg / L.
Description
Technical field
The present invention relates to agricultural biological technical field, particularly, relate to a kind of preparation method of torch tree fruit extract.
Background technology
The metabolic disease of diabetes to be one group with hyperglycemia be feature.Hyperglycemia be then due to defect of insulin secretion or its biological agent impaired, or both have concurrently and cause.Long-standing hyperglycemia during diabetes, causes various tissue, particularly eye, kidney, heart, blood vessel, neural chronic lesion, dysfunction.
At present, the oral class medicine thing for the treatment of diabetes comprises sulfonylurea drugs, biguanides, α glucosidase inhibitor, euglycemic agent and meglitinide Insulin secretagogues.Wherein, α glucosidase inhibitor is the oral paradiabetes medicine of a class of outbalance.Acarbose is the α glucosidase inhibitor had been found that, but after as Remedies for diabetes, has occurred certain drug resistance phenomenon.
In order to solve the drug resistance problems of acarbose, just need to find the acarbose substitute with α glucosidase inhibitory active.Had been found that some plant extracts have α glucosidase inhibitory active, but the α glucosidase inhibitory active of these extracts is still not high, therefore medical value is lower.
Summary of the invention
The object of this invention is to provide a kind of preparation method of torch tree fruit extract, to prepare the torch tree fruit extract with higher α glucosidase inhibitory active.
The present inventor finds, is extracted by torch tree fruit ethanol water and is extracted with ethyl acetate, can obtain the extract with higher α glucosidase inhibitory active, resulting in the present invention under Ultrasonic Conditions.
To achieve these goals, the invention provides a kind of preparation method of torch tree fruit extract, the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtains distilling residue, then will distill residue and mix with water, and obtain material to be extracted, and be extracted with ethyl acetate, and be extracted thing and remove the ethyl acetate in extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10-30L, and extracting ethyl acetate used is 5-10L.
By technique scheme, the torch tree fruit extract that the preparation method of torch tree fruit extract of the present invention prepares can have higher α glucosidase inhibitory active, the IC of its Inhibiting α-glucosidase activity
50value is at below 2500mg/L.
Other features and advantages of the present invention are described in detail in detailed description of the invention part subsequently.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail.Should be understood that, detailed description of the invention described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
In the present invention, when not doing contrary explanation, the liquid of use or the volumetric quantities of gas are all 20 DEG C, the numerical value of 1 standard atmosphere pressure.
The invention provides a kind of preparation method of torch tree fruit extract, the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtains distilling residue, then will distill residue and mix with water, and obtain material to be extracted, and be extracted with ethyl acetate, and be extracted thing and remove the ethyl acetate in extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10-30L, and extracting ethyl acetate used is 5-10L.
Wherein, in step (1), dry condition can be the drying condition that fruit drying is commonly used, and such as, dry condition can make the water content of torch tree fruit dry powder be 5-10 % by weight; Such as, dry temperature can be 40-70 DEG C.
Wherein, in step (1), the condition of pulverizing can be the conventional drying condition of fruit drying, and preferably, the condition of pulverizing makes torch tree fruit dry powder can by 60 mesh sieves.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %.
Wherein, in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation can be 20-100W, the time of Ultrasonic Radiation can be 30-60 minute, and the frequency of Ultrasonic Radiation can be 25-55kHz, and the temperature of Ultrasonic Radiation can be 20-35 DEG C.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C.
Wherein, in step (4), the temperature of the ethanol in the distillation removing described stillness of night can be 40-50 DEG C.
Wherein, in order to improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract further, preferably, in step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L.
Wherein, as the particularly preferred a kind of embodiment of the present invention, in step (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L.In this preferred implementation, further can improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract.
Describe the present invention by the following examples.
Embodiment 1
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 20L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 7.5L.Organic facies extraction obtained as extract, and is distilled at 45 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 2
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 65 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 40W, the time of ultrasonic Treatment is 40 minutes, the frequency of ultrasonic Treatment is 35kHz, the temperature of ultrasonic Treatment is 25 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 7L.Organic facies extraction obtained as extract, and is distilled at 42 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 3
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 75 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 15L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 60W, the time of ultrasonic Treatment is 50 minutes, the frequency of ultrasonic Treatment is 45kHz, the temperature of ultrasonic Treatment is 30 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 25L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 8L.Organic facies extraction obtained as extract, and is distilled at 48 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 4
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 50 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 20L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 100W, the time of ultrasonic Treatment is 60 minutes, the frequency of ultrasonic Treatment is 55kHz, the temperature of ultrasonic Treatment is 20 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 30L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 10L.Organic facies extraction obtained as extract, and is distilled at 50 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Embodiment 5
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 90 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 20W, the time of ultrasonic Treatment is 30 minutes, the frequency of ultrasonic Treatment is 25kHz, the temperature of ultrasonic Treatment is 35 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 5L.Organic facies extraction obtained as extract, and is distilled at 42 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that the present embodiment obtains.
Comparative example 1
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
To distill residue to mix with water, and obtain material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 100L.And be extracted with ethyl acetate described material to be extracted, relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, extracting ethyl acetate used is 3L.Organic facies extraction obtained as extract, and is distilled at 45 DEG C, the ethyl acetate content in extract is down to less than 0.1 % by weight, obtains cream solid.
Then be less than 1 % by weight by cream solid lyophilization to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that this comparative example obtains.
Comparative example 2
The method identical with embodiment 1 is adopted to prepare torch tree fruit extract, unlike, ethyl acetate is changed to n-butyl alcohol.
Comparative example 3
The method identical with embodiment 1 is adopted to prepare torch tree fruit extract, unlike, ethyl acetate is changed to chloroform.
Comparative example 4
It is 7 % by weight that fresh torch tree fruit is dried to water content under 60 DEG C of hot blasts, and being then crushed to pulverizer can by the torch tree fruit dry powder of 60 mesh sieves.Mixed with extracting solution (ethanol waters of 70 volume %) by described torch tree fruit dry powder, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 12L, obtains mixed material.By described mixed material ultrasonic Treatment, relative to often liter by the material of ultrasonic Treatment, the power of ultrasonic Treatment is 50W, the time of ultrasonic Treatment is 45 minutes, the frequency of ultrasonic Treatment is 40kHz, the temperature of ultrasonic Treatment is 28 DEG C, by the centrifugal 10min under the centrifugal speed condition of 3000 × g of the material after ultrasonic Treatment, obtains clear liquid.Clear liquid is distilled at 45 DEG C, the ethanol content in clear liquid is reduced to less than 0.1 % by weight, obtain distilling residue.
Then be less than 1 % by weight by the lyophilization of distillation residue to water content at-50 DEG C, obtain solid dry powder.This solid dry powder is the torch tree fruit extract that this comparative example obtains.
Testing example 1
According to document (Yao, Y.; Sang, W.; Zhou, M.J.; Ren, G.X.Antioxidant and α-Glucosidase inhibitory activity of colored grains in China.Journal ofAgricultural and Food Chemistry.2010, method 58:770-774), measures the IC of the Inhibiting α-glucosidase activity of the torch tree fruit extract that embodiment 1-5 and comparative example 1-4 obtains
50value, result is as shown in table 1.The output of the antioxidant content product that the torch tree fruit dry powder calculating every 1kg in the method for embodiment 1-5 and comparative example 1-3 can extract, result is as shown in table 1.
Table 1
As can be seen from Table 1, the torch tree fruit extract that the preparation method of torch tree fruit extract of the present invention prepares has higher α glucosidase inhibitory active.Further, in preferred steps (2), relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, when to extract ethyl acetate used be 7-8L, further can improve productive rate and the α glucosidase inhibitory active of torch tree fruit extract.
Testing example 2
Mice (male mice in kunming, cleaning grade, weight 18-22g) after fasting 18h, lumbar injection streptozotocin (80mg/kg), after 72h and 144 hour, same dose is injected once more respectively, after injecting two weeks first, and fasted mice 3h, and get tail vein survey fasting blood sugar, choosing fasting plasma glucose concentration is the mice suffering from diabetes the mice of 12-24mmol/L.
The torch tree fruit extract obtained by embodiment 1-5 and comparative example 1-4 is the gavage liquid of 50mg/mL respectively with water dissolution, the mice of then exchanging diabetes respectively with the every gavage amount only of 1mL carries out gavage (often organizing 10), continuous gavage 10 days, after last gavage, fasting 1h and 2h gets tail vein respectively and surveys blood glucose value, and result is as shown in table 2.
Table 2
From the data of table 2, the torch tree fruit extract that method of the present invention obtains can reduce the blood glucose value of the mice suffering from diabetes effectively, has effect of blood sugar lowering thus, can be used in preparing hypoglycemic medicine.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned detailed description of the invention, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible compound mode.
In addition, also can carry out combination in any between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (9)
1. a preparation method for torch tree fruit extract, is characterized in that: the method comprises:
(1) torch tree fruit is dry and pulverize, obtain torch tree fruit dry powder;
(2) mixed with extracting solution by described torch tree fruit dry powder, obtain mixed material, relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 5-20L, and extracting solution is the ethanol water of 50-90 volume %;
(3) by the process of described mixed material Ultrasonic Radiation, and remove the solid in the material after Ultrasonic Radiation process, obtain clear liquid;
(4) ethanol in the distillation removing described stillness of night, obtains distilling residue, then will distill residue and mix with water, and obtain material to be extracted, and be extracted with ethyl acetate, and be extracted thing and remove the ethyl acetate in extract; Relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 10-30L, and extracting ethyl acetate used is 5-10L.
2. preparation method according to claim 1, is characterized in that: in step (1), and dry condition makes the water content of torch tree fruit dry powder be 5-10 % by weight.
3. preparation method according to claim 1, is characterized in that: in step (1), and the condition of pulverizing makes torch tree fruit dry powder can by 60 mesh sieves.
4. preparation method according to claim 1, is characterized in that: in step (2), and relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %.
5. preparation method according to claim 1, it is characterized in that: in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 20-100W, the time of Ultrasonic Radiation is 30-60 minute, the frequency of Ultrasonic Radiation is 25-55kHz, and the temperature of Ultrasonic Radiation is 20-35 DEG C.
6. preparation method according to claim 5, it is characterized in that: in step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, the time of Ultrasonic Radiation is 40-50 minute, the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C.
7. preparation method according to claim 1, is characterized in that: in step (4), and the temperature of the ethanol in the distillation removing described stillness of night is 40-50 DEG C.
8. preparation method according to claim 1, it is characterized in that: in step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L.
9. preparation method according to claim 1, is characterized in that: in step (2), and relative to the torch tree fruit dry powder of 1kg, the consumption of extracting solution is 8-15L, and extracting solution is the ethanol water of 65-75 volume %; In step (3), relative to often liter by the material of Ultrasonic Radiation, the power of Ultrasonic Radiation is 40-60W, and the time of Ultrasonic Radiation is 40-50 minute, and the frequency of Ultrasonic Radiation is 35-45kHz, and the temperature of Ultrasonic Radiation is 25-30 DEG C; In step (4), relative to the distillation residue that the torch tree fruit dry powder of 1kg obtains, the consumption of water makes the volume of material to be extracted be 15-25L, and extracting ethyl acetate used is 7-8L.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107287266A (en) * | 2017-08-03 | 2017-10-24 | 吉林大学 | Torch tree fruit extract, preparation method and its application in gene engineering product fermenting and producing |
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| US4654214A (en) * | 1985-01-23 | 1987-03-31 | Martinetti Patrick J | Method of treating respiratory disease in animals |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107287266A (en) * | 2017-08-03 | 2017-10-24 | 吉林大学 | Torch tree fruit extract, preparation method and its application in gene engineering product fermenting and producing |
| CN107287266B (en) * | 2017-08-03 | 2020-08-07 | 吉林大学 | Torch tree fruit extract, preparation method and application thereof in fermentation production of genetic engineering products |
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