CN102224829A - Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases - Google Patents
Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases Download PDFInfo
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- CN102224829A CN102224829A CN2011101045896A CN201110104589A CN102224829A CN 102224829 A CN102224829 A CN 102224829A CN 2011101045896 A CN2011101045896 A CN 2011101045896A CN 201110104589 A CN201110104589 A CN 201110104589A CN 102224829 A CN102224829 A CN 102224829A
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Abstract
Belonging to the technical field of pesticide preparation, the invention relates to an albizzia julibrissin durazzini extract and a method for preparing a water agent with the extract to control melon diseases. The preparation method is characterized by comprising the following steps orderly: colleting, air-drying and crushing albizzia julibrissin durazzini, then adding ethanol as a solvent, thus obtaining an ethanol crude extract of albizzia julibrissin durazzini; concentrating the crude extract under reduced pressure so as to obtain albizzia julibrissin durazzini extractum; adding distilled water, and placing the mixture in an ultrasonic generator to make the extractum suspended; conducting extraction with isopyknic petroleum ether, ethyl acetate and normal butanol in order for 5 times respectively, and incorporating the extract liquors of normal butanol, concentrating the incorporated extract liquor under reduced pressure till dry, thus obtaining the albizzia julibrissin durazzini extract; finally preparing a water agent containing 0.2-4% of the albizzia julibrissin durazzini extract for controlling melon diseases. The water agent prepared by the method of the invention is a pollution-free botanical biological bacteriacide, which shows a high control efficiency to melon fusarium wilt, melon gray mold, melon leaf blight, melon sclerotinia and the like. And the water agent prepared in the invention has widely distributed raw materials and abundant resources, simple extraction process, no pollution to the environment, little harm and residual hazard to people and livestock.
Description
Technical field
The invention belongs to the preparation technique of pesticide field, relate to the preparation method of a kind of silktree albizia bark extract and control muskmelon disease aqua thereof.
Background technology
Muskmelon contains the welcome that rich in protein, carbohydrate, vitamin and mineral matter etc. enjoy the domestic and international market because of its fruit, and muskmelon fusarium wilt, muskmelon gray mold, muskmelon leaf rot and muskmelon stalk break are the main diseases in the muskmelon production, generally cause production loss l0-15 %, especially muskmelon fusarium wilt, the highest underproduction can reach more than 70 %.At present to the control of this disease still based on chemical control, and long-term a large amount of chemical pesticide that uses, causing 3R(is the rampant again of pest resistance, residue of pesticide, pest) problem become the focus of people's common concern.
Silk tree (Albizia julibrissin Durazz.) belongs to leguminous plant, and the middle part wide geographic area all has plantation in China, is to beautify the environment, and purifies air and the fine tree species of medical value.Bibliographical information contains number of chemical compositions such as triterpene, flavones, lignanoid, alkaloid, tannin and polysaccharide in the cortex albiziae.Wherein cortex albiziae and flower are Chinese medicine commonly used, and the effect of controlling confused and worried melancholy insomnia, activating blood circulation and reducing swelling is arranged.
Yang Xiujuan etc. have reported that silk tree leaf ethanol extract has control efficiency to root-knot nematode in " plant protection journal " (2004 the 4th phases) is entitled as the article of " eelworm-killing activity of several plant and control efficiency thereof "; Is CN1709067A for people such as radiance at publication number, and name is called in the Chinese patent of " method for preparing control cucumber and tomato disease aqua with albizzia julibrissin extract ", mentions with silk tree leaf extract control cucumber downy mildew and graw mold of tomato; Yin Qisheng etc. are called in publication number CN101731286A, name and find in the Chinese patent of " a kind of albizzia julibrissin extracting solution and application thereof " that the silk tree leaf extract has stronger antiviral activity to tobacco mosaic virus; Lv Jinshun etc. publication number CN101461386A, name be called mention in the Chinese patent of " bastard tamarind legume extract and preparation method thereof and antibacterial agent " with the antibacterial agent of bastard tamarind legume extract preparation inhibited to Escherichia coli, staphylococcus aureus, salmonella, bacillus megaterium, hay bacillus, pseudomonas aeruginosa.But also there is not silktree albizia bark extract to be used to prevent and treat the research report of plant diseases such as muskmelon at present.
Summary of the invention
The objective of the invention is at above shortcomings in the prior art, the preparation method of a kind of silktree albizia bark extract and control muskmelon disease aqua thereof is provided, utilize Nantural non-toxic plant silktree albizia bark extract to prepare aqua control muskmelon disease, for reducing the use of chemical pesticide, produce green or organic melon and fruit technical support is provided.
The present invention is achieved by the following technical solutions, and the preparation method of a kind of silktree albizia bark extract and control muskmelon disease aqua thereof is characterized in that comprising the steps: successively
(1) after the collection cortex albiziae dried and crushed, in mass volume ratio, 70%～100% ethanol that adding is equivalent to 5～20 times of volumes of Silktree Albizia Bark quality leaves standstill dipping after fully stirring at ambient temperature and extracts 3～5 times as solvent, each 24 hours, merging obtained the cortex albiziae ethanol crude extract;
(2) ethanol crude extract that step (1) is obtained is concentrated into paste with the method for concentrating under reduced pressure with it, gets cortex albiziae medicinal extract;
(3) add the distilled water that is equivalent to 5 times of volumes of Silktree Albizia Bark quality in the cortex albiziae medicinal extract, place supersonic generator that medicinal extract is suspended then; Respectively extract 5 times with isopyknic benzinum, ethyl acetate, n-butanol successively again; Discard benzinum and acetic acid ethyl acetate extract, merge butanol extraction liquid and dried, promptly get silktree albizia bark extract through being evaporated to;
(4) by weight percentage, with the silktree albizia bark extract preparation control muskmelon disease aqua that distilled water or deionized water dissolving step (3) obtain, silktree albizia bark extract accounts for 0.2～4 % in the described aqua, and surplus is a water.
In the described step (1), described pulverizing is meant with medicinal herb grinder and is ground into 10～50 purpose plant powders.
In described step (2) and the step (3), described concentrating under reduced pressure is meant solution vacuumized with the cycle water vavuum pump and is decompressed to-0.1 atmospheric pressure, places the rotary water bath pot below 30～50 ℃, makes solution concentration, ethanol crude extract is concentrated into paste, and butanol extraction liquid is concentrated into dried.
In the described step (3), describedly respectively extract 5 times with isopyknic benzinum, ethyl acetate, n-butanol successively, promptly with petroleum ether extraction 5 times, 5 times, last extracting n-butyl alcohol 5 times of using ethyl acetate extraction again.
The present invention compared with prior art has following advantage: the aqua that the present invention makes is a kind of pollution-free plant source biological bactericide, and diseases such as muskmelon fusarium wilt, muskmelon gray mold, muskmelon leaf rot and muskmelon stalk break are had higher control efficiency; The cortex albiziae feed distribution is wide, aboundresources, is easy to artificial cultivation; Extraction process is simple, need not special instrument, and the solvent after the recovery can reuse, and environmentally safe is to harmless, the no residual hazard of person poultry safety.
Embodiment
Embodiment 1:
Gather cortex albiziae and dry under 30～35 ℃, be ground into 20 purpose plant powders with medicinal herb grinder, take by weighing Silktree Albizia Bark 50 g, extract 3 times with 70 % ethanol, 250 mL room temperatures dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets medicinal extract 3.1 g; Utilize ultrasonic, with 250 mL water gained medicinal extract is suspended, respectively extract successively 5 times with equal-volume (250 mL) benzinum, ethyl acetate, n-butanol, promptly with petroleum ether extraction 5 times, ethyl acetate extraction 5 times, use extracting n-butyl alcohol 5 times again, discard benzinum and acetic acid ethyl acetate extract, merge n-butyl alcohol extract and dried, obtain extract 0.4 g through being evaporated to.Filter with 100 mL dissolved in distilled water extracts, be mixed with the aqua that concentration is 0.2 %, adopt growth rate method that muskmelon fusarium wilt, muskmelon gray mold, muskmelon leaf rot and muskmelon stalk break are carried out indoor antibacterial test, suppress effect and be followed successively by 56.3 %, 56.0 %, 55.0 % and 68.4 %.
Embodiment 2:
Take by weighing Silktree Albizia Bark (10 order particle) 500 g, with 85 % ethanol, 5000 mL room temperatures, dipping extracts 4 times, and each 24 hours, concentrating under reduced pressure reclaimed solvent and gets medicinal extract 37.2 g; Utilize ultrasonic, gained medicinal extract is suspended, use equal-volume (2500 mL) benzinum, ethyl acetate, n-butanol respectively to extract successively 5 times, merge n-butyl alcohol extract and dried, obtain extract 6.1 g through being evaporated to 2500 mL water; Filter with 200 mL dissolved in distilled water extracts, be mixed with the aqua that concentration is 2 %.
Indoor with the artificial infection of muskmelon wilt behind muskmelon seedling root, every strain melon seedling is irritated described aqua 50 mL of root, 3 all " Invest, Then Investigate "s morbidity strain numbers, obtaining control efficiency is 71.0 %; Indoor with muskmelon ash arrhizus bacteria, muskmelon leaf spoting bacteria and the artificial infection of muskmelon sclerotium germ after the muskmelon seedling leaves, to every strain melon seedling complete stool described aqua 30 mL that spray, 2 all " Invest, Then Investigate " blade occurring degrees also calculate disease index, obtain control efficiency and are followed successively by 66.2 %, 65.5 % and 67.2 %.
Embodiment 3:
Take by weighing Silktree Albizia Bark (50 order particle) 500 g, extract 5 times with 100 % ethanol, 10000 mL room temperatures dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets medicinal extract 35.9 g; Utilize ultrasonic, with 2500 mL water medicinal extract is suspended, equal-volume benzinum, ethyl acetate, n-butanol respectively extract 5 times successively, merge n-butyl alcohol extract and be evaporated to dried, obtain extract 6.4 g, filter, be mixed with the aqua that concentration is 4 % with 100 mL dissolved in distilled water extracts.
In the muskmelon fusarium wilt field controling test, every strain melon seedling is irritated described aqua 150 mL of root, 4 all " Invest, Then Investigate " morbidity strain numbers, obtaining control efficiency is 51.9 %; In muskmelon gray mold, muskmelon leaf rot and muskmelon stalk break field controling test, to every strain melon seedling complete stool described aqua 50 mL that spray, 2 all " Invest, Then Investigate " blade occurring degrees also calculate disease index, obtain control efficiency at last and are followed successively by 51.9 %, 66.1 %, 47.1 % and 61.7 %.
Claims (3)
1. the preparation method of silktree albizia bark extract and control muskmelon disease aqua thereof is characterized in that comprising the steps: successively
(1) after the collection cortex albiziae dried and crushed, in mass volume ratio, 70%～100% ethanol that adding is equivalent to 5～20 times of volumes of Silktree Albizia Bark quality leaves standstill dipping after fully stirring at ambient temperature and extracts 3～5 times as solvent, each 24 hours, merging obtained the cortex albiziae ethanol crude extract;
(2) ethanol crude extract that step (1) is obtained is concentrated into paste with the method for concentrating under reduced pressure with it, gets cortex albiziae medicinal extract;
(3) in cortex albiziae medicinal extract, add the distilled water that is equivalent to 5 times of volumes of Silktree Albizia Bark quality, place supersonic generator that medicinal extract is suspended then; Respectively extract 5 times with isopyknic benzinum, ethyl acetate, n-butanol successively again; Discard benzinum and acetic acid ethyl acetate extract, merge butanol extraction liquid and dried, promptly get silktree albizia bark extract through being evaporated to;
(4) by weight percentage, with the silktree albizia bark extract preparation control muskmelon disease aqua that distilled water or deionized water dissolving step (3) obtain, silktree albizia bark extract accounts for 0.2～4 % in the described aqua, and surplus is a water.
2. according to the preparation method of described a kind of silktree albizia bark extract of claim 1 and control muskmelon disease aqua thereof, it is characterized in that in the described step (1) that described pulverizing is meant with medicinal herb grinder and is ground into 10～50 purpose plant powders.
3. according to the preparation method of described a kind of silktree albizia bark extract of claim 1 and control muskmelon disease aqua thereof, it is characterized in that in described step (2) and the step (3), described concentrating under reduced pressure is meant solution vacuumized and is decompressed to-0.1 atmospheric pressure, place the rotary water bath pot below 30～50 ℃, make solution concentration.
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| CN2011101045896A CN102224829A (en) | 2011-04-26 | 2011-04-26 | Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases |
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| CN2011101045896A CN102224829A (en) | 2011-04-26 | 2011-04-26 | Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464347A (en) * | 2018-03-21 | 2018-08-31 | 江西农业大学 | A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent |
| CN115067127A (en) * | 2022-07-29 | 2022-09-20 | 四川佳猕沃农业科技有限公司 | Biological control method for kiwifruit canker |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709067A (en) * | 2005-06-30 | 2005-12-21 | 上海交通大学 | Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract |
| CN101564052A (en) * | 2009-05-21 | 2009-10-28 | 上海交通大学 | Preparation method of aqua for preventing and controlling sclerotia rot of colza |
| CN101766675A (en) * | 2008-12-30 | 2010-07-07 | 江南大学 | Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application |
-
2011
- 2011-04-26 CN CN2011101045896A patent/CN102224829A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709067A (en) * | 2005-06-30 | 2005-12-21 | 上海交通大学 | Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract |
| CN101766675A (en) * | 2008-12-30 | 2010-07-07 | 江南大学 | Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application |
| CN101564052A (en) * | 2009-05-21 | 2009-10-28 | 上海交通大学 | Preparation method of aqua for preventing and controlling sclerotia rot of colza |
Non-Patent Citations (1)
| Title |
|---|
| 赵杰 等: "植物提取液对甜瓜枯萎病的抑菌活性及种子萌发的影响", 《上海交通大学学报( 农业科学版)》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464347A (en) * | 2018-03-21 | 2018-08-31 | 江西农业大学 | A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent |
| CN115067127A (en) * | 2022-07-29 | 2022-09-20 | 四川佳猕沃农业科技有限公司 | Biological control method for kiwifruit canker |
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Application publication date: 20111026 |