CN108464347A - A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent - Google Patents

A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent Download PDF

Info

Publication number
CN108464347A
CN108464347A CN201810233297.4A CN201810233297A CN108464347A CN 108464347 A CN108464347 A CN 108464347A CN 201810233297 A CN201810233297 A CN 201810233297A CN 108464347 A CN108464347 A CN 108464347A
Authority
CN
China
Prior art keywords
parts
citrus
antistaling agent
bark extract
albizia bark
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810233297.4A
Other languages
Chinese (zh)
Inventor
万春鹏
陈楚英
陈金印
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangxi Agricultural University
Original Assignee
Jiangxi Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangxi Agricultural University filed Critical Jiangxi Agricultural University
Priority to CN201810233297.4A priority Critical patent/CN108464347A/en
Publication of CN108464347A publication Critical patent/CN108464347A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/16—Coating with a protective layer; Compositions or apparatus therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to citrus post-harvest fresh-keeping technical fields, rear plant antistaling agent is adopted more particularly to a kind of citrus comprising silktree albizia bark extract, the antistaling agent includes 6.25 50 parts of silktree albizia bark extracts, 0.5 2.0 parts of film forming agents, 0.5 1.0 parts of cosolvents, 0.5 1.5 portions of preservatives, 0.5 1.0 parts of pH value adjustment agent, 0.5 2.0 parts of osmotic pressure regulators, 0.5 3.0 parts of antioxidants and 1000 parts of water.The present invention prepares a kind of novel citrus post-harvest fresh-keeping agent by principle active component of natural Chinese medicinal herb silktree albizia bark extract, make full use of the apparent advantage of silktree albizia bark extract fungistatic effect, rationally, other auxiliary elements of the collocation of science, the antistaling agent is set to have both of good preservation effect, no chemical residues, to human body without health hazard, it is environmentally safe the features such as, there is good application value.

Description

A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent
Technical field
The present invention relates to citrus post-harvest fresh-keeping technical field, more particularly to after a kind of citrus comprising silktree albizia bark extract is adopted Plant antistaling agent.
Background technology
Citrus is the first in the world major class fruit, is the second major class fruit in addition to apple in China, and be only second to wheat With the third-largest international trade agricultural product of corn.Citrus postharvest decay is the main problem during orange storing and circulation sale One of.The microorganism disease type up to more than 20 that the citrus postharvest storage phase occurs is planted, by Italian mould (Penicillium ) and Penicillium italicum (Blue mould) and green mold caused by Penicillium digitatum (Penicillium digitatum) italicum Sick (Green mould) is two kinds of citrus postharvest diseases for being easiest to occur, and the rotting rate that citrus adopts rear 90% is sick by both Evil causes.Therefore, the research of citrus postharvest storage preservation technology is paid attention to by more and more people.Chemical method is to apply at present Most commonly used citrus postharvest handling method, but it be easy to cause the toxic side effect of Fungicide residue.In recent years, from edible plants Natural plants based bacteriostat is found in resource adopts the height that rear anti-corrosive fresh-keeping receives countries in the world researcher for citrus Concern.
Cortex albiziae (cortex albiziae) is the dry bark of legume silk tree, is common Chinese medicine simply, in China Most area has plantation.Chemical composition and pharmacological experiment study show in cortex albiziae containing triterpenes, flavonoids, biology The compounds such as bases, lignanoid's glycoside have the effects that antitumor and cell toxicant, tranquilizing soporific antidepression, Immunity regulation.
Currently, the bacteriostatic activity to silk tree is studied primarily directed to silk tree leaf, Flos Albiziae or bastard tamarind legume, for silk tree The bacteriostatic activity research of skin is less.Studies have reported that bastard tamarind legume extract, to gold-coloured staphylococci, Escherichia coli and verdigris are false Monad have preferable bacteriostatic activity (bacteriostasis research [J] the modern foods science and technology of the old firm bastard tamarind legume extracts of length, 2013,29(2):274-276.)；The common Marine Pathogenic Bacteria Vibrio anguillarum of five kinds of Flos Albiziae extracting solution pair, Vibrio harveyi, brilliance Vibrios, Gaul vibrios and bacillus megaterium have inhibiting effect (Wang Yizhuo, Luo Hui, the virtuous silk trees flowers volatile oil chemistry of scholar Zhao at Point and the research of extracting solution bacteriostasis [J] hubei agricultural sciences, 2012,51 (6):1245-1247)；Silk tree stem-leaf extract pair Cotton-wilt fusarium, Pythium ultimum germ and cotton rhizoctonia solani have preferable bacteriostatic activity (Jia Fuli, Chen Yijuan, Chen Jia, king Dan Dan, research [J] Shanghai communications university's journals of generation radiance silk trees 8 kinds of plant pathogenic fungi bacteriostatic activities of stem-leaf extract pair (agricultural sciences version), 2011,29 (4):32-37)；Silk tree leaf ethanol extract is continuous to apple decay bacterium, Rhizoctonia solani Kuhn, tomato The plant pathogenic fungis such as maize ear rot bacterium, tomato gray mould bacterium, Rhizoctonia cereali, gibberella saubinetii, capsicum anthrax-bacilus have certain suppression It makes of (chicory, Qu Tianli, Xiao Lin silk tree leaf ethanol extracts are in Antibacterial Activity [J] of plant pathogenic fungi State's agronomy notification, 2009,25 (3):202-205.), Extract of alcoholized Albizia cortex is dense to the minimum inhibition of staphylococcus aureus It is 20mg/mL to spend (MIC), is 12.43mg/mL to concentration (EC50) in the inhibition of aspergillus niger, preferable to aspergillus niger fungistatic effect (Bai Xianqin, generation radiance, Chen Jia, Chen Yijuan, Pang Qianru, research [J] of Guo Song silktree albizia bark extracts to aspergillus niger bacteriostatic activity Food industry science and technology, 2014,35 (12):133-136.).
Patent CN201110104589.6 proposes the preparation of a kind of silktree albizia bark extract and its control muskmelon disease aqua Method can be applied to the prevention of Muskmelon Fusarium wilt, gray mold, leaf blight and sclerotiniose.Patent CN200510027334.9 is proposed A kind of method that albizzia julibrissin extract prepares control cucumber and tomato disease aqua, can be applied to prevention cucumber downy mildew and tomato Gray mold.
In conclusion not yet finding to adopt the report of rear plant antistaling agent about the citrus comprising silktree albizia bark extract at present. Based on the above reason, the present invention develops a kind of citrus comprising silktree albizia bark extract and adopts rear plant antistaling agent, for after the adopting of citrus The new way of fresh-keeping offer.
Invention content
In order to overcome the above-mentioned deficiencies of the prior art, the present invention is provided after a kind of citrus comprising silktree albizia bark extract is adopted and is planted The features such as object antistaling agent, this antistaling agent have fungistatic effect apparent, no chemical residues, non-environmental-pollution.
To achieve the goals above, the technical solution adopted in the present invention is：
A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent, includes the ingredient of following parts by weight：
6.25-50 parts of silktree albizia bark extract, 0.5-2.0 parts of film forming agent, 0.5-1.0 parts of cosolvent, preservative 0.5-1.5 Part, PH
It is worth 0.5-1.0 parts of conditioning agent, 0.5-2.0 parts of osmotic pressure regulator, 0.5-3.0 parts of antioxidant, 1000 parts of water.
Preferably, the antistaling agent includes the ingredient of following parts by weight：
50 parts of silktree albizia bark extract, 1.5 parts of film forming agent, 0.8 part of cosolvent, 1.0 parts of preservative, pH value adjustment agent 0.8
Part, 1.0 parts of osmotic pressure regulator, 1.2 parts of antioxidant, 1000 parts of water.
Preferably, the cosolvent is selected from least one of glycerine, sucrose ester, TWEEN Series, and the preservative is selected from mountain At least one of potassium sorbate, sodium benzoate, the pH value adjustment agent is at least one in citric acid, acetic acid, lactic acid and tartaric acid Kind, the osmotic pressure regulator is selected from least one of calcium propionate, calcium chloride, sodium chloride, calcium carbonate, and the antioxidant is selected from Vitamin C, tea polyphenols, the water are sterile water.
Preferably, the film forming agent is selected from least one of sodium carboxymethylcellulose, sodium alginate, chitosan, chitin.
Preferably, the osmotic pressure regulator is selected from least one of calcium propionate, calcium chloride, sodium chloride, calcium carbonate.
It is further preferred that the antistaling agent further includes the ingredient of following parts by weight：
1.0-1.5 parts of plant growth regulator.
Still more preferably, the plant growth regulator is selected from least one in 2,4- dichlorphenoxyacetic acids, abscisic acid Kind.
Preferably, liquor or nanoemulsions can be made in the antistaling agent, and the antistaling agent is directly sprayed or is applied to The surface for adopting rear citrus carries out fresh-keeping.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) present invention is prepared by principle active component of natural Chinese medicinal herb silktree albizia bark extract after a kind of novel citrus adopts Antistaling agent makes full use of the apparent advantage of silktree albizia bark extract fungistatic effect, rationally, the collocation of science other auxiliary elements, makes The antistaling agent has both of good preservation effect, no chemical residues, to human body without health hazard, it is environmentally safe the features such as, have very Good application value；
(2) the citrus post-harvest fresh-keeping agent pair that the present invention is prepared using natural Chinese medicinal herb silktree albizia bark extract as principle active component The biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium and green mould experiments have shown that, which is respectively to the biocontrol effect of penicilliosis and green mould 6.04%-45.91% and 14.29%-55.37% obtains good control effect, especially more to the prevention of green mould Significantly.
Specific implementation mode
The specific implementation mode of the present invention is described further below.It should be noted that for these implementations The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.In addition, invention described below Involved technical characteristic can be combined with each other as long as they do not conflict with each other in each embodiment.
Embodiment 1：
A kind of citrus comprising silktree albizia bark extract adopts life of the rear plant antistaling agent to citrus Postharvest Penicillium and green mould Anti- effect
1, experiment material：
1. for reagent object：Including the citrus of silktree albizia bark extract adopts rear plant antistaling agent, the formula of the antistaling agent is：
6.25 parts of silktree albizia bark extract, 0.5 part of sodium carboxymethylcellulose, 0.5 part of glycerine, 0.5 part of potassium sorbate, citric acid 0.5 part, 0.5 part of calcium propionate, 0.5 part of vitamin C, 1000 parts of water.
2. strains tested：Italian mould (P.italicum, ACCC30399), Penicillium digitatum (P.digitatum, ACCC30389) it is all from Chinese agriculture Culture Collection (ACCC).
2, experimental method：
It rinses well, dries spare after the 2%NaClO solution disinfections processing of Xinyu tangerine fruit, then with aqua sterilisa.It is real Before testing, a diameter 4mm, the wound of depth 3mm are stabbed in fruit waist with disinfection transfer needle, experiment is divided into two parts：
(1) citrus comprising silktree albizia bark extract is adopted rear plant antistaling agent and is tested to the biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Italy of CFU/mL is green Mould spore suspension is inoculated into the wound of citrusfruit, as positive control.
(2) citrus comprising silktree albizia bark extract adopts the biological and ecological methods to prevent plant disease, pests, and erosion experiment that rear plant antistaling agent adopts citrus rear green mould：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Penicillium digitatum of CFU/mL Spore suspension is inoculated into the wound of citrusfruit, as positive control；
Finally, the fruit after handling well being placed on pallet, puts on bag film, respectively handle 6 fruits, experiment is repeated 3 times, After keeping relative humidity to cultivate culture 7d at 95% or so, 27 DEG C, citrusfruit lesion diameter is investigated, is calculated according to following formula Control effect.
Control effect (%)=100 × (positive controls be averaged lesion diameter-antistaling agent processing group be averaged lesion diameter)/ Positive controls lesion diameter
3, experimental result：
(1) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent to citrus Postharvest Penicillium With obvious control effect, such as table 1, after handling 7d to citrus, the control effect to Italian penicilliosis is 6.04%.
A kind of citrus comprising silktree albizia bark extract of table 1 adopts control effect of the rear plant antistaling agent to Penicillium italicum
(2) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent adopts rear green mould to citrus With obvious control effect, such as table 2, after handling 7d to citrus, the control effect to green mould is 14.29%.
A kind of citrus comprising silktree albizia bark extract of table 2 adopts control effect of the rear plant antistaling agent to citrus common green mold
Embodiment 2：
A kind of citrus comprising silktree albizia bark extract adopts life of the rear plant antistaling agent to citrus Postharvest Penicillium and green mould Anti- effect
1, experiment material：
1. for reagent object：Including the citrus of silktree albizia bark extract adopts rear plant antistaling agent, the formula of the antistaling agent is：
25 parts of silktree albizia bark extract, film forming agent (press 1 by sodium carboxymethylcellulose and sodium alginate：1 composition) 1.0 parts, sugarcane 0.8 part of sugar ester, 1.0 parts of potassium sorbate, 0.7 part of acetic acid, 1.0 parts of calcium chloride, 1.2 parts of vitamin C, 1000 parts of water.
2. strains tested：Italian mould (P.italicum, ACCC30399), Penicillium digitatum (P.digitatum, ACCC30389) it is all from Chinese agriculture Culture Collection (ACCC).
2, experimental method：
It rinses well, dries spare after the 2%NaClO solution disinfections processing of Xinyu tangerine fruit, then with aqua sterilisa.It is real Before testing, a diameter 4mm, the wound of depth 3mm are stabbed in fruit waist with disinfection transfer needle, experiment is divided into two parts：
(1) citrus comprising silktree albizia bark extract is adopted rear plant antistaling agent and is tested to the biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Italy of CFU/mL is green Mould spore suspension is inoculated into the wound of citrusfruit, as positive control；
(2) citrus comprising silktree albizia bark extract adopts the biological and ecological methods to prevent plant disease, pests, and erosion experiment that rear plant antistaling agent adopts citrus rear green mould：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Penicillium digitatum of CFU/mL Spore suspension is inoculated into the wound of citrusfruit, as positive control；
Finally, the fruit after handling well being placed on pallet, puts on bag film, respectively handle 6 fruits, experiment is repeated 3 times, After keeping relative humidity to cultivate culture 7d at 95% or so, 27 DEG C, citrusfruit lesion diameter is investigated, is calculated according to following formula Control effect.
Control effect (%)=100 × (positive controls be averaged lesion diameter-antistaling agent processing group be averaged lesion diameter)/ Positive controls lesion diameter
3, experimental result：
(1) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent to citrus Postharvest Penicillium With obvious control effect, such as table 1, after handling 7d to citrus, the control effect to Italian penicilliosis is 31.21%.
A kind of citrus comprising silktree albizia bark extract of table 1 adopts control effect of the rear plant antistaling agent to Penicillium italicum
(2) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent adopts rear green mould to citrus With obvious control effect, such as table 2, after handling 7d to citrus, the control effect to green mould is 40.99%.
A kind of citrus comprising silktree albizia bark extract of table 2 adopts control effect of the rear plant antistaling agent to citrus common green mold
Embodiment 3：
A kind of citrus comprising silktree albizia bark extract adopts life of the rear plant antistaling agent to citrus Postharvest Penicillium and green mould Anti- effect
1, experiment material：
1. for reagent object：Including the citrus of silktree albizia bark extract adopts rear plant antistaling agent, the formula of the antistaling agent is：
50 parts of silktree albizia bark extract, film forming agent (press 1 by sodium alginate, chitosan and chitin：1：1 composition) 1.5 parts, sugarcane 0.9 part of sugar ester, 1.2 parts of sodium benzoate, 0.85 part of lactic acid, osmotic pressure regulator (press 1 by calcium chloride, sodium chloride and calcium carbonate：1： 1 composition) 1.6 parts, 2.0 parts of tea polyphenols, 1000 parts of water.
2. strains tested：Italian mould (P.italicum, ACCC30399), Penicillium digitatum (P.digitatum, ACCC30389) it is all from Chinese agriculture Culture Collection (ACCC).
2, experimental method：
It rinses well, dries spare after the 2%NaClO solution disinfections processing of Xinyu tangerine fruit, then with aqua sterilisa.It is real Before testing, a diameter 4mm, the wound of depth 3mm are stabbed in fruit waist with disinfection transfer needle, experiment is divided into two parts：
(1) citrus comprising silktree albizia bark extract is adopted rear plant antistaling agent and is tested to the biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Italy of CFU/mL is green Mould spore suspension is inoculated into the wound of citrusfruit, as positive control.
(2) citrus comprising silktree albizia bark extract adopts the biological and ecological methods to prevent plant disease, pests, and erosion experiment that rear plant antistaling agent adopts citrus rear green mould：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Penicillium digitatum of CFU/mL Spore suspension is inoculated into the wound of citrusfruit, as positive control.
Finally, the fruit after handling well being placed on pallet, puts on bag film, respectively handle 6 fruits, experiment is repeated 3 times, After keeping relative humidity to cultivate culture 7d at 95% or so, 27 DEG C, citrusfruit lesion diameter is investigated, is calculated according to following formula Control effect.
Control effect (%)=100 × (positive controls be averaged lesion diameter-antistaling agent processing group be averaged lesion diameter)/ Positive controls lesion diameter
3, experimental result：
(1) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent to citrus Postharvest Penicillium With obvious control effect, such as table 1, after handling 7d to citrus, the control effect to Italian penicilliosis is 44.07%.
A kind of citrus comprising silktree albizia bark extract of table 1 adopts control effect of the rear plant antistaling agent to Penicillium italicum
(2) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent adopts rear green mould to citrus With obvious control effect, such as table 2, after handling 7d to citrus, the control effect to green mould is 54.03%.
A kind of citrus comprising silktree albizia bark extract of table 2 adopts control effect of the rear plant antistaling agent to citrus common green mold
Embodiment 4：
A kind of citrus comprising silktree albizia bark extract adopts life of the rear plant antistaling agent to citrus Postharvest Penicillium and green mould Anti- effect
1, experiment material：
1. for reagent object：Including the citrus of silktree albizia bark extract adopts rear plant antistaling agent, the formula of the antistaling agent is：
6.25 parts of silktree albizia bark extract, 0.5 part of carboxymethyl cellulose, 0.5 part of glycerine, 0.5 part of potassium sorbate, citric acid 0.5 part, 0.5 part of calcium propionate, 0.5 part of vitamin C, 1.0 parts of 2,4 dichlorophenoxyacetic acid, 1000 parts of water.
2. strains tested：Italian mould (P.italicum, ACCC30399), Penicillium digitatum (P.digitatum, ACCC30389) it is all from Chinese agriculture Culture Collection (ACCC).
2, experimental method：
It rinses well, dries spare after the 2%NaClO solution disinfections processing of Xinyu tangerine fruit, then with aqua sterilisa.It is real Before testing, a diameter 4mm, the wound of depth 3mm are stabbed in fruit waist with disinfection transfer needle, experiment is divided into two parts：
(1) citrus comprising silktree albizia bark extract is adopted rear plant antistaling agent and is tested to the biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL；
2. directly by 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL is inoculated into the wound of citrusfruit, As positive control.
(2) citrus comprising silktree albizia bark extract adopts the biological and ecological methods to prevent plant disease, pests, and erosion experiment that rear plant antistaling agent adopts citrus rear green mould：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL；
2. directly by 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL is inoculated into the wound of citrusfruit, makees For positive control.
Finally, the fruit after handling well being placed on pallet, puts on bag film, respectively handle 6 fruits, experiment is repeated 3 times, After keeping relative humidity to cultivate culture 7d at 95% or so, 27 DEG C, citrusfruit lesion diameter is investigated, is calculated according to following formula Control effect.
Control effect (%)=100 × (positive controls be averaged lesion diameter-antistaling agent processing group be averaged lesion diameter)/ Positive controls lesion diameter
3, experimental result：
(1) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent to citrus Postharvest Penicillium With obvious control effect, such as table 1, after handling 7d to citrus, the control effect to Italian penicilliosis is 7.21%.
A kind of citrus comprising silktree albizia bark extract of table 1 adopts control effect of the rear plant antistaling agent to Penicillium italicum
(2) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent adopts rear green mould to citrus With obvious control effect, such as table 2, after handling 7d to citrus, the control effect to green mould is 15.16%.
A kind of citrus comprising silktree albizia bark extract of table 2 adopts control effect of the rear plant antistaling agent to citrus common green mold
Embodiment 5：
A kind of citrus comprising silktree albizia bark extract adopts life of the rear plant antistaling agent to citrus Postharvest Penicillium and green mould Anti- effect
1, experiment material：
1. for reagent object：Including the citrus of silktree albizia bark extract adopts rear plant antistaling agent, the formula of the antistaling agent is：
50 parts of silktree albizia bark extract, film forming agent (press 1 by sodium alginate, chitosan and chitin：1：1 composition) 1.5 parts, sugarcane 0.9 part of sugar ester, 1.2 parts of sodium benzoate, 0.85 part of lactic acid, osmotic pressure regulator (press 1 by calcium chloride, sodium chloride and calcium carbonate：1： 1 composition) 1.6 parts, 2.0 parts of tea polyphenols, plant growth regulator (press 1 by 2,4 dichlorophenoxyacetic acid and abscisic acid：1 composition) 1.5 parts, 1000 parts of water.
2. strains tested：Italian mould (P.italicum, ACCC30399), Penicillium digitatum (P.digitatum, ACCC30389) it is all from Chinese agriculture Culture Collection (ACCC).
2, experimental method：
It rinses well, dries spare after the 2%NaClO solution disinfections processing of Xinyu tangerine fruit, then with aqua sterilisa.It is real Before testing, a diameter 4mm, the wound of depth 3mm are stabbed in fruit waist with disinfection transfer needle, experiment is divided into two parts：
(1) citrus comprising silktree albizia bark extract is adopted rear plant antistaling agent and is tested to the biological and ecological methods to prevent plant disease, pests, and erosion of citrus Postharvest Penicillium：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Italian mould spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Italy of CFU/mL is green Mould spore suspension is inoculated into the wound of citrusfruit, as positive control.
(2) citrus comprising silktree albizia bark extract adopts the biological and ecological methods to prevent plant disease, pests, and erosion experiment that rear plant antistaling agent adopts citrus rear green mould：
1. be first inoculated with the wound that 20 μ L antistaling agents handle citrusfruit with micropipettor, then inoculate 20 μ L 1 × 106The Penicillium digitatum spore suspension of CFU/mL；
2. 20 μ L sterile waters to be inoculated into the wound of citrusfruit, then by 20 μ L 1 × 106The Penicillium digitatum of CFU/mL Spore suspension is inoculated into the wound of citrusfruit, as positive control.
Finally, the fruit after handling well being placed on pallet, puts on bag film, respectively handle 6 fruits, experiment is repeated 3 times, After keeping relative humidity to cultivate culture 7d at 95% or so, 27 DEG C, citrusfruit lesion diameter is investigated, is calculated according to following formula Control effect.
Control effect (%)=100 × (positive controls be averaged lesion diameter-antistaling agent processing group be averaged lesion diameter)/ Positive controls lesion diameter
3, experimental result：
(1) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent to citrus Postharvest Penicillium With obvious control effect, such as table 1, after handling 7d to citrus, the control effect to Italian penicilliosis is 45.91%.
A kind of citrus comprising silktree albizia bark extract of table 1 adopts control effect of the rear plant antistaling agent to Penicillium italicum
(2) the experimental results showed that, including the citrus of silktree albizia bark extract adopts rear plant antistaling agent adopts rear green mould to citrus With obvious control effect, such as table 2, after handling 7d to citrus, the control effect to green mould is 55.37%.
A kind of citrus comprising silktree albizia bark extract of table 2 adopts control effect of the rear plant antistaling agent to citrus common green mold
Embodiments of the present invention are explained in detail above, but the present invention is not limited to described embodiments.It is right For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more Kind change, modification, replacement and modification, still fall in protection scope of the present invention.

Claims (8)

1. a kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent, it is characterised in that：The antistaling agent includes following The ingredient of parts by weight：
6.25-50 parts of silktree albizia bark extract, 0.5-2.0 parts of film forming agent, 0.5-1.0 parts of cosolvent, 0.5-1.5 parts of preservative, PH It is worth 0.5-1.0 parts of conditioning agent, 0.5-2.0 parts of osmotic pressure regulator, 0.5-3.0 parts of antioxidant, 1000 parts of water.
2. a kind of citrus comprising silktree albizia bark extract according to claim 1 adopts rear plant antistaling agent, it is characterised in that： The antistaling agent includes the ingredient of following parts by weight：
50 parts of silktree albizia bark extract, 1.5 parts of film forming agent, 0.8 part of cosolvent, 1.0 parts of preservative, 0.8 part of pH value adjustment agent, infiltration Press 1.0 parts of conditioning agent, 1.2 parts of antioxidant, 1000 parts of water.
3. a kind of citrus comprising silktree albizia bark extract according to claim 1 or 2 adopts rear plant antistaling agent, feature exists In：The cosolvent is selected from least one of glycerine, sucrose ester, TWEEN Series, and the preservative is selected from potassium sorbate, benzoic acid At least one of sodium, the pH value adjustment agent are selected from least one of citric acid, acetic acid, lactic acid and tartaric acid, the osmotic pressure Conditioning agent is selected from least one of calcium propionate, calcium chloride, sodium chloride, calcium carbonate, and it is more that the antioxidant is selected from vitamin C, tea Phenol, the water are sterile water.
4. a kind of citrus comprising silktree albizia bark extract according to claim 1 or 2 adopts rear plant antistaling agent, feature exists In：The film forming agent is selected from least one of sodium carboxymethylcellulose, sodium alginate, chitosan, chitin.
5. a kind of citrus comprising silktree albizia bark extract according to claim 1 or 2 adopts rear plant antistaling agent, feature exists In：The osmotic pressure regulator is selected from least one of calcium propionate, calcium chloride, sodium chloride, calcium carbonate.
6. a kind of citrus comprising silktree albizia bark extract according to claim 1 or 2 adopts rear plant antistaling agent, feature exists In：The antistaling agent further includes the ingredient of following parts by weight：1.0-1.5 parts of plant growth regulator.
7. a kind of citrus comprising silktree albizia bark extract according to claim 6 adopts rear plant antistaling agent, it is characterised in that： The plant growth regulator is selected from least one of 2,4 dichlorophenoxyacetic acid, abscisic acid.
8. a kind of citrus comprising silktree albizia bark extract according to claim 1 adopts rear plant antistaling agent, it is characterised in that： Liquor or nanoemulsions can be made in the antistaling agent, and the antistaling agent is directly sprayed to or is applied to the table for adopting rear citrus Face carries out fresh-keeping.
CN201810233297.4A 2018-03-21 2018-03-21 A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent Pending CN108464347A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810233297.4A CN108464347A (en) 2018-03-21 2018-03-21 A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810233297.4A CN108464347A (en) 2018-03-21 2018-03-21 A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent

Publications (1)

Publication Number Publication Date
CN108464347A true CN108464347A (en) 2018-08-31

Family

ID=63265500

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810233297.4A Pending CN108464347A (en) 2018-03-21 2018-03-21 A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent

Country Status (1)

Country Link
CN (1) CN108464347A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110810091A (en) * 2019-12-06 2020-02-21 南昌大学 Fruit and vegetable fresh-keeping method
CN113455541A (en) * 2021-06-21 2021-10-01 三峡大学 Citrus preservative

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101300978A (en) * 2008-06-23 2008-11-12 中国农业科学院柑桔研究所 Preparation for preventing orange fruit blossom end from yellowing and shedding and preparation and use method thereof
CN102132725A (en) * 2010-01-22 2011-07-27 张永锋 Environment-friendly preservative for fruits and vegetables
CN102224829A (en) * 2011-04-26 2011-10-26 上海市浦东新区农业技术推广中心 Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases
CN103404537A (en) * 2013-08-23 2013-11-27 江西农业大学 Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101300978A (en) * 2008-06-23 2008-11-12 中国农业科学院柑桔研究所 Preparation for preventing orange fruit blossom end from yellowing and shedding and preparation and use method thereof
CN102132725A (en) * 2010-01-22 2011-07-27 张永锋 Environment-friendly preservative for fruits and vegetables
CN102224829A (en) * 2011-04-26 2011-10-26 上海市浦东新区农业技术推广中心 Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases
CN103404537A (en) * 2013-08-23 2013-11-27 江西农业大学 Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周梦娇: "桂枝提取物对柑橘采后主要病原真菌抑菌活性的研究"，周梦娇，农业科技辑，第46-155页", 《桂枝提取物对柑橘采后主要病原真菌抑菌活性的研究》 *
白羡钦: "合欢皮提取物对黑曲霉抑菌活性的研究", 《食品工业科技》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110810091A (en) * 2019-12-06 2020-02-21 南昌大学 Fruit and vegetable fresh-keeping method
CN110810091B (en) * 2019-12-06 2022-04-12 南昌大学 Fruit and vegetable fresh-keeping method
CN113455541A (en) * 2021-06-21 2021-10-01 三峡大学 Citrus preservative

Similar Documents

Publication Publication Date Title
CN104146055A (en) Plant source fruit and vegetable preservative
CN106343010A (en) Vegetable preservative containing plant essential oil and application
CN106615085A (en) Microorganism and plant derived composite fresh keeping agent for fruits and vegetables
Mohammadi et al. Integration between chitosan and Zataria multiflora or Cinnamomum zeylanicum essential oil for controlling Phytophthora drechsleri, the causal agent of cucumber fruit rot
CN105105281A (en) Natural food preservative
JPH04316506A (en) Agent for contorolling plant-injuring organism
Marino et al. Chitosan-propolis combination inhibits anthracnose in'Hass' avocados
Gallo et al. Applications of chitosan as a functional food
CN108419828A (en) A kind of citrus comprising loquat-leaf extract adopts rear plant antistaling agent
CN108991105A (en) A kind of apple fresh-keeping agent and preparation method thereof containing garlic active material
EP1871395B1 (en) Garlic extract and chitosan compositions, and uses thereof
Goñi et al. Clove (Syzygium aromaticum) oils
CN108464347A (en) A kind of citrus comprising silktree albizia bark extract adopts rear plant antistaling agent
CN106258659A (en) A kind of preventing control method of Hylocereus undatus postharvest disease
CN103053626A (en) Natural lemon disinfectant and preparation method thereof
KR101253712B1 (en) Chitosan and chitooligosaccharide composition for preventing and disease control of plant disease and method for increase of plant growth and disease control of plant by using the same
CN104855496B (en) A kind of method for adopting rear citrus anti-corrosive fresh-keeping
CN103734274A (en) Novel composite aquatic product fresh-keeping agent, and preparation method thereof
CN104413073A (en) Mould-proof care agent for clothes
CN110037102A (en) A kind of prevention and treatment fruit adopts the preparation of rear mould disease
CN107771917A (en) A kind of osmunda polysaccharides tomato pr eservation agent
CN107232238A (en) A kind of application of litsea cubeba oil on control capsicum epidemic disease
CN111802448A (en) Compound plant essential oil sterilization preservative and preparation method thereof
CN106359558A (en) Natural cherry fresh keeping agent and preparation method thereof
KR20090048902A (en) Soaking liquid of seed for sprout-vegetable

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20180831

RJ01 Rejection of invention patent application after publication