CN111202756A - Preparation method of cacumen biotae ethanol extract - Google Patents
Preparation method of cacumen biotae ethanol extract Download PDFInfo
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- CN111202756A CN111202756A CN201811391260.0A CN201811391260A CN111202756A CN 111202756 A CN111202756 A CN 111202756A CN 201811391260 A CN201811391260 A CN 201811391260A CN 111202756 A CN111202756 A CN 111202756A
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- Prior art keywords
- ethanol
- filtrate
- extract
- cacumen biotae
- ethanol extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of an ethanol extract of cacumen biotae, which comprises the following steps: (1) weighing 3 parts of cacumen biotae powder 10.0 g in a 250mL conical flask; (2) respectively adding 150mL of 50%, 70% and 95% ethanol according to a certain material-liquid ratio, and soaking at constant temperature for a certain time; (3) performing ultrasonic treatment in an ultrasonic cleaner (power 500W, frequency 50kHz, temperature 70 ℃) for a certain time, filtering under reduced pressure, putting the filtrate in an evaporating dish, and concentrating in a water bath at 7 ℃ until the filtrate is dry to obtain an extract; (4) accurately weighing 0.25g of ethanol extracts with different concentrations, dissolving with 50%, 70% and 95% ethanol, and fixing volume in a 250mL volumetric flask. The arborvitae tops ethanol extract provided by the invention also contains antioxidant active ingredients for removing and reducing damage of free radicals, so that the arborvitae tops ethanol extract is concerned in preventing and treating diseases.
Description
Technical Field
The invention relates to the technical field of biological extraction, and particularly relates to a preparation method of an ethanol extract of cacumen biotae.
Background
Cacumen biotae, name of traditional Chinese medicine. Is dried shoot and leaf of Platycladus orientalis of Cupressaceae. Collected in summer and autumn mostly and dried in the shade.
It has many branches and flat branches. The leaves are fine and scaly, alternately opposite, attached to the branches, dark green or yellow green. Is brittle and easy to break. Fragrant smell, bitter and astringent taste and slight pungent taste. Cool blood to stop bleeding, resolve phlegm to stop cough, promote hair growth and blacken hair. Can be used for treating hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, metrostaxis, cough due to lung heat, alopecia due to blood heat, and premature gray hair.
Disclosure of Invention
Based on the above objects, the present invention provides a method for preparing an ethanol extract of cacumen biotae, which comprises:
(1) weighing 10.0 g of 3 parts of oriental arborvitae powder in a 250m L conical bottle;
(2) mixing the raw materials in a ratio of 1: 15 (g/m L) respectively adding 50%, 70% and 95% ethanol 150m L, and soaking at 20 deg.C for 4 h;
(3) performing ultrasonic treatment in an ultrasonic cleaner (power 500W, frequency 50kHz, temperature 70 ℃) for 60m in, filtering under reduced pressure, putting the filtrate in an evaporating dish, and concentrating in water bath at 7 ℃ to obtain extract;
(4) accurately weighing 0.25g of ethanol extracts with different concentrations, dissolving with 50%, 70% and 95% ethanol, and adding into 250m L volumetric flask.
Free radicals are molecules or ions with unpaired electrons and are highly reactive. Excessive free radical oxidation and intermediate products thereof seriously damage the functions of biological membranes, enzymes, proteins and living cells, and cause diseases such as tumors, heart and brain ischemia, atherosclerosis and the like. In order to mitigate the harm of free radicals, the research of antioxidants is one of the hot spots today. Although the existing artificially synthesized antioxidant has remarkable effect, the safety = performance of the antioxidant has some problems. The arborvitae tops ethanol extract provided by the invention also contains antioxidant active ingredients for removing and reducing damage of free radicals, so that the arborvitae tops ethanol extract is concerned in preventing and treating diseases.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
(1) Weighing 3 parts of cacumen biotae powder 10.0 g in a 250m L conical flask;
(2) mixing the raw materials in a ratio of 1: 15 (g/m L) respectively adding 50%, 70% and 95% ethanol 150m L, and soaking at 20 deg.C for 4 h;
(3) performing ultrasonic treatment in an ultrasonic cleaner (power 500W, frequency 50kHz, temperature 70 ℃) for 60min, filtering under reduced pressure, putting the filtrate in an evaporating dish, and concentrating in water bath at 7 ℃ to obtain extract;
(4) accurately weighing 0.25g of ethanol extracts with different concentrations, dissolving with 50%, 70% and 95% ethanol, and adding into 250m L volumetric flask.
Example 2
(1) Weighing 3 parts of cacumen biotae powder 10.0 g in a 250m L conical flask;
(2) mixing the raw materials in a ratio of 1: adding 50%, 70% and 95% ethanol 150m L at a ratio of 30 (g/m L) respectively, and soaking at 20 deg.C for 4 h;
(3) performing ultrasonic treatment in an ultrasonic cleaner (power 500W, frequency 50kHz, temperature 70 ℃) for 60min, filtering under reduced pressure, putting the filtrate in an evaporating dish, and concentrating in water bath at 7 ℃ to obtain extract;
(4) accurately weighing 0.25g of ethanol extracts with different concentrations, dissolving with 50%, 70% and 95% ethanol, and adding into 250m L volumetric flask.
Claims (5)
1. The preparation method of the roselle extractive is characterized by comprising the following steps:
(1) weighing 10.0 g of 3 parts of oriental arborvitae powder in a 250m L conical bottle;
(2) respectively adding 50%, 70% and 95% ethanol 150m L according to a certain ratio of material to liquid, and soaking for a certain time at a constant temperature;
(3) ultrasonically treating in an ultrasonic cleaner (power 500W, frequency 50kHz, temperature 70 ℃) for a certain time, filtering under reduced pressure, putting the filtrate in an evaporating dish, and concentrating in a water bath at 7 ℃ until the filtrate is dry to obtain an extract;
(4) accurately weighing 0.25g of ethanol extracts with different concentrations, dissolving with 50%, 70% and 95% ethanol, and adding into 250m L volumetric flask.
2. The method according to claim 1, wherein the ratio of the feed to the liquid in step (2) is 1: 15.
3. The constant temperature in step (2) according to claim 1 is 20 ℃.
4. The soaking time in the step (2) according to claim 1 is 4 hours.
5. The sonication time in step (3) according to claim 1 is 60 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391260.0A CN111202756A (en) | 2018-11-21 | 2018-11-21 | Preparation method of cacumen biotae ethanol extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391260.0A CN111202756A (en) | 2018-11-21 | 2018-11-21 | Preparation method of cacumen biotae ethanol extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111202756A true CN111202756A (en) | 2020-05-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811391260.0A Pending CN111202756A (en) | 2018-11-21 | 2018-11-21 | Preparation method of cacumen biotae ethanol extract |
Country Status (1)
| Country | Link |
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| CN (1) | CN111202756A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111658580A (en) * | 2020-06-23 | 2020-09-15 | 广西和桂集团有限公司 | Hair growth and/or hair blackening essence containing various peptides and plant extracts and preparation method thereof |
| CN112120990A (en) * | 2020-07-22 | 2020-12-25 | 广州市圣莎拉化妆品有限公司 | Plant type anti-hair loss and hair growth promoting product based on cacumen biotae and processing and extracting process |
-
2018
- 2018-11-21 CN CN201811391260.0A patent/CN111202756A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111658580A (en) * | 2020-06-23 | 2020-09-15 | 广西和桂集团有限公司 | Hair growth and/or hair blackening essence containing various peptides and plant extracts and preparation method thereof |
| CN112120990A (en) * | 2020-07-22 | 2020-12-25 | 广州市圣莎拉化妆品有限公司 | Plant type anti-hair loss and hair growth promoting product based on cacumen biotae and processing and extracting process |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200529 |