KR20230015793A - A method for manufacturing cypress leaf extract for removing the smell of the elderly - Google Patents
A method for manufacturing cypress leaf extract for removing the smell of the elderly Download PDFInfo
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- 239000012046 mixed solvent Substances 0.000 claims description 8
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 21
- 230000003078 antioxidant effect Effects 0.000 abstract description 20
- 238000004332 deodorization Methods 0.000 abstract description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 39
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- 238000010438 heat treatment Methods 0.000 description 5
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- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
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- 229910052802 copper Inorganic materials 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q15/00—Anti-perspirants or body deodorants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
Description
본 발명은 노인냄새 제거를 위한 편백나무 잎 추출물의 제조방법에 관한 것으로서, 보다 상세하게는 (a) 편백나무 잎을 세척하는 단계; (b) 상기 세척된 편백나무 잎 및 용매를 혼합하고, 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 열수 추출하여 추출액을 제조하는 단계; 및 (c) 상기 추출액을 감압 농축하여 추출물을 수득하는 단계를 포함하는 편백나무 잎 추출물의 제조방법에 관한 것이다.The present invention relates to a method for preparing a cypress leaf extract for removing the smell of old age, and more particularly, (a) washing the cypress tree leaves; (b) preparing an extract by mixing the washed cypress leaves and a solvent and performing hot water extraction at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa for 30 minutes to 10 hours; And (c) it relates to a method for producing a cypress leaf extract comprising the step of obtaining an extract by concentrating the extract under reduced pressure.
나이가 들어가면서 젊을 때 나지 않던 냄새가 발생하는데, 이는 2-노네날이라는 물질 때문이다. 특히 40대 후반부터 몸에서 냄새가 나기 시작하여 80대에서는 상당한 양의 냄새가 발생한다. As you get older, you get a smell that you didn't smell when you were young, and this is because of a substance called 2-nonenal. In particular, the body starts to smell from the late 40s, and a considerable amount of odor occurs in the 80s.
2-노네날은 피지 속의 지방산이 산화되면서 생성되어 털이 자라는 부위인 모공에 쌓이고 부패하면서 퀴퀴한 냄새를 풍긴다. 2-nonenal is produced when fatty acids in sebum are oxidized, accumulates in pores, where hair grows, and gives off a musty smell as it decays.
한편 식물 유래의 다양한 화학물질들은 소취성, 냄새제거특성, 항산화성, 항균성, 노화방지, 항 골다공증 작용 등을 포함한 여러 가지 생리활성들을 나타내어 의약품 및 건강기능식품의 유용한 소재로서 각광을 받고 있다. On the other hand, various plant-derived chemical substances are in the limelight as useful materials for pharmaceuticals and health functional foods because they exhibit various physiological activities including deodorization, odor removal properties, antioxidant, antibacterial, anti-aging, and anti-osteoporosis actions.
편백나무에는 피톤치드라는 천연 항균물질이 함유되어 있어 살균작용이 우수하고, 내수성이 강해 물에 닿으면 고유의 향이 진하게 퍼져 냄새를 잡아주는 역할을 할 수 있다. Cypress trees contain a natural antibacterial substance called phytoncide, which has excellent sterilization and strong water resistance.
이와 관련하여 한국공개특허 제10-2019-0031983호는 (a) 편백나무 잎을 구리로 이루어진 증류솥에 투입하는 단계; (b) 상기 편백나무 잎이 투입된 증류솥을 30 내지 100 ℃의 온도로 가열하여 증기를 포집하는 단계; 및 (c) 상기 포집된 증기를 냉각시켜 편백나무 추출물을 수득하는 단계;를 포함하는 것을 특징으로 하는 편백나무 잎 추출물의 제조방법을 개시하고 있다. In this regard, Korean Patent Publication No. 10-2019-0031983 discloses (a) putting cypress leaves into a distillation pot made of copper; (b) collecting steam by heating the distillation pot into which the cypress leaves were added at a temperature of 30 to 100 ° C; And (c) cooling the collected steam to obtain a cypress tree extract; discloses a method for producing a cypress tree leaf extract comprising the.
그러나 상기 문헌에 개시된 기술은 소취성, 냄새제거특성, 수득율, 항산화성, 항균성 등의 특성을 향상시킬 수 있는 편백나무 잎 추출물의 제조방법에 대해서는 개시하고 있지 않다. However, the technology disclosed in the above document does not disclose a method for preparing a cypress leaf extract capable of improving properties such as deodorization, odor removal, yield, antioxidant, and antibacterial properties.
따라서 편백나무 잎으로부터 소취성, 냄새제거특성, 수득율, 항산화성, 항균성 등의 특성을 극대화할 수 있는 편백나무 잎 추출물의 제조방법에 대한 기술개발이 필요하다. Therefore, it is necessary to develop a technology for manufacturing a cypress leaf extract capable of maximizing properties such as deodorization, odor removal, yield, antioxidant, and antibacterial properties from cypress tree leaves.
본 발명은 상기 종래 기술의 문제점을 해결하기 위한 것으로서, 쉽고 간단하게 제조할 수 있으며 소취성, 노인냄새 제거특성, 수득율, 항산화성, 항균성 등의 특성을 극대화할 수 있는 편백나무 잎 추출물의 제조방법을 제공하는 것을 목적으로 한다. The present invention is to solve the problems of the prior art, a method for producing a cypress leaf extract that can be easily and simply produced and can maximize properties such as deodorization, old odor removal, yield, antioxidant, and antibacterial properties is intended to provide
상기와 같은 목적을 달성하기 위하여 본 발명은 (a) 편백나무 잎을 세척하는 단계; In order to achieve the above object, the present invention includes (a) washing cypress leaves;
(b) 상기 세척된 편백나무 잎 및 용매를 혼합하고, 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 열수 추출하여 추출액을 제조하는 단계; 및 (b) preparing an extract by mixing the washed cypress leaves and a solvent and performing hot water extraction at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa for 30 minutes to 10 hours; and
(c) 상기 추출액을 감압 농축하여 추출물을 수득하는 단계를 포함하는 편백나무 잎 추출물의 제조방법을 제공한다. (c) provides a method for producing a cypress leaf extract comprising the step of obtaining an extract by concentrating the extract under reduced pressure.
본 발명의 일실시예에 있어서, 상기 단계 (b)는 초음파를 인가하여 추출액을 제조하는 것을 특징으로 한다. In one embodiment of the present invention, the step (b) is characterized in that the extract is prepared by applying ultrasonic waves.
본 발명의 일실시예에 있어서, 상기 용매는 에탄올 및 물의 혼합용매인 것을 특징으로 한다. In one embodiment of the present invention, the solvent is characterized in that a mixed solvent of ethanol and water.
본 발명의 일실시예에 있어서, 상기 에탄올 및 물의 중량비는 15~45:55~85 인 것을 특징으로 한다. In one embodiment of the present invention, the weight ratio of the ethanol and water is characterized in that 15 ~ 45: 55 ~ 85.
또한 본 발명은 상기 제조방법으로 제조되는 편백나무 잎 추출물을 제공한다. In addition, the present invention provides a cypress leaf extract prepared by the above production method.
본 발명은 쉽고 간단하게 제조할 수 있으며 소취성, 노인냄새 제거특성, 수득율, 항산화성, 항균성 등의 특성을 극대화할 수 있는 편백나무 잎 추출물의 제조방법을 제공할 수 있다. The present invention can provide a method for preparing a cypress leaf extract that can be easily and simply produced and can maximize properties such as deodorization, old odor removal, yield, antioxidant, and antibacterial properties.
도 1은 편백나무 잎 추출물의 항균 시험 결과를 나타낸다.
도 2는 편백나무 잎 추출물의 항산화 시험 결과를 나타낸다. Figure 1 shows the antibacterial test results of cypress leaf extract.
Figure 2 shows the antioxidant test results of the cypress tree leaf extract.
이하 실시예를 바탕으로 본 발명을 상세히 설명한다. 본 발명에 사용된 용어, 실시예 등은 본 발명을 보다 구체적으로 설명하고 통상의 기술자의 이해를 돕기 위하여 예시된 것에 불과할 뿐이며, 본 발명의 권리범위 등이 이에 한정되어 해석되어서는 안 된다. The present invention will be described in detail based on the following examples. The terms, examples, etc. used in the present invention are merely exemplified to explain the present invention in more detail and help the understanding of those skilled in the art, and the scope of the present invention should not be construed as being limited thereto.
본 발명에 사용되는 기술 용어 및 과학 용어는 다른 정의가 없다면 이 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 통상적으로 이해하고 있는 의미를 나타낸다. Technical terms and scientific terms used in the present invention represent meanings commonly understood by those of ordinary skill in the art to which this invention belongs, unless otherwise defined.
본 발명은 (a) 편백나무 잎을 세척하는 단계; The present invention comprises the steps of (a) washing cypress leaves;
(b) 상기 세척된 편백나무 잎 및 용매를 혼합하고, 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 열수 추출하여 추출액을 제조하는 단계; 및 (b) preparing an extract by mixing the washed cypress leaves and a solvent and performing hot water extraction at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa for 30 minutes to 10 hours; and
(c) 상기 추출액을 감압 농축하여 추출물을 수득하는 단계를 포함하는 편백나무 잎 추출물의 제조방법에 관한 것이다. (c) It relates to a method for producing a cypress leaf extract comprising the step of obtaining an extract by concentrating the extract under reduced pressure.
상기 단계 (a)는 편백나무 잎을 세척하는 단계로서, 채취된 편백나무 잎은 적당한 크기로 절단하여 사용할 수 있으며, 필요에 따라 분말화하여 사용할 수도 있다. The step (a) is a step of washing the cypress leaves, and the collected cypress leaves can be cut into appropriate sizes and used, or, if necessary, powdered.
상기 편백나무 잎을 흐르는 물이나 스팀 분사법으로 세척하여 표면에 존재하는 농약이나 각종 이물질을 제거한다. The cypress leaves are washed with flowing water or steam spraying to remove pesticides or various foreign substances present on the surface.
세척한 편백나무 잎은 건조하지 않고 그대로 사용하거나 또는 건조가 이루어지는 건조수단에 투입하여 수분을 일정 함량 이하로 조절할 수 있다. 건조방법으로는 열풍건조, 냉풍건조, 동결건조, 상온건조, 진공건조 등이 있다. The washed cypress leaves can be used as they are without drying or put into a drying means in which drying is performed to adjust the moisture content to a certain level or less. Drying methods include hot air drying, cold air drying, freeze drying, room temperature drying, and vacuum drying.
상기 단계 (b)는 상기 세척된 편백나무 잎 및 용매를 혼합하고, 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 열수 추출하여 추출액을 제조하는 단계로서, 상기 세척된 편백나무 잎을 고압에서 열수 추출하여 추출액을 제조할 수 있다. The step (b) is a step of preparing an extract by mixing the washed cypress leaves and the solvent and extracting hot water for 30 minutes to 10 hours at a temperature of 40 to 150 ° C and a pressure of 0.5 to 10 MPa to prepare an extract. Extracts can be prepared by hot water extraction of tree leaves under high pressure.
상기 편백나무 잎을 물, 알코올(에탄올, 메탄올, 주정, 발효주정 등), 아세톤 등의 용매로 가열 추출하여 추출액을 제조할 수 있다. An extract may be prepared by heating and extracting the cypress leaves with a solvent such as water, alcohol (ethanol, methanol, alcohol, fermented alcohol, etc.) or acetone.
가열 추출은 편백나무 잎 100중량부에 대하여 용매 100~2,000중량부를 가하고 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 가열하여 추출할 수 있다. Heat extraction can be extracted by adding 100 to 2,000 parts by weight of a solvent based on 100 parts by weight of cypress leaves and heating for 30 minutes to 10 hours at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa.
본 발명은 용매로서 에탄올 및 물의 혼합용매를 사용하는 것이 바람직하다. 이때 상기 에탄올 및 물의 중량비는 15~45:55~85 인 것이 바람직하다. 에탄올 및 물의 중량비가 상기 수치범위를 만족하는 경우, 소취성, 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 극대화될 수 있다. In the present invention, it is preferable to use a mixed solvent of ethanol and water as a solvent. At this time, the weight ratio of the ethanol and water is preferably 15 to 45:55 to 85. When the weight ratio of ethanol and water satisfies the above numerical range, deodorization, old odor removal, yield, antibacterial and antioxidant properties can be maximized.
또한 본 발명은 용매로서 에탄올, 메탄올 및 물의 혼합용매를 사용하는 것이 바람직하다. 이때 상기 에탄올, 메탄올 및 물의 중량비는 30~60:20~40:100 인 것이 바람직하다. 에탄올, 메탄올 및 물의 중량비가 상기 수치범위를 만족하는 경우, 소취성, 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 극대화될 수 있다. In addition, in the present invention, it is preferable to use a mixed solvent of ethanol, methanol and water as a solvent. At this time, the weight ratio of the ethanol, methanol and water is preferably 30 to 60:20 to 40:100. When the weight ratio of ethanol, methanol and water satisfies the above numerical range, deodorization, old odor removal, yield, antibacterial and antioxidant properties can be maximized.
본 발명은 추출액을 제조하는 단계에서 초음파를 인가할 수 있으며, 상기 초음파를 10분~2시간 사용함으로써 제조된 추출액의 소취성, 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 극대화될 수 있다. The present invention can apply ultrasonic waves in the step of preparing the extract, and by using the ultrasonic waves for 10 minutes to 2 hours, the deodorization, old odor removal, yield, antibacterial and antioxidant properties of the prepared extract can be maximized.
상기 단계 (c)는 상기 추출액을 감압 농축하여 추출물을 수득하는 단계로서, 상기 추출액을 여과막 등으로 여과하여 여액을 수득한 후, 이를 감압 농축하여 추출물을 제조할 수 있다. The step (c) is a step of obtaining an extract by concentrating the extract under reduced pressure. The extract may be filtered through a filtration membrane to obtain a filtrate, and then concentrated under reduced pressure to prepare an extract.
상기 추출액은 열을 가하면서 농축하거나 진공 하에서 농축하여 점도가 높은 상태의 추출물을 제조하거나 분말 형태의 추출물을 제조할 수 있다. The extract may be concentrated while applying heat or concentrated under vacuum to prepare an extract with high viscosity or an extract in powder form.
또한 본 발명은 상기 (c) 단계 이후에, 상기 제조된 추출물을 정제수로 수용화하여 수용성 추출물을 제조하는 단계 (d)를 추가로 포함할 수 있다. In addition, the present invention may further include a step (d) of preparing a water-soluble extract by solubilizing the prepared extract in purified water after step (c).
상기 수용성 추출물은 수용화 과정을 거치면서 유효성분의 함량이 증가하고 항균성 및 항산화 특성이 향상될 수 있다. The water-soluble extract may increase the content of active ingredients and improve antibacterial and antioxidant properties while undergoing a water-solubilization process.
상기 수용성 추출물은 그대로 사용하거나 감압 농축하여 정제수를 제거하여 사용할 수 있다. The water-soluble extract may be used as it is or concentrated under reduced pressure to remove purified water.
또한 본 발명은 상기 (c) 단계에서 제조된 추출물 및 상기 (d) 단계에서 제조된 수용성 추출물을 혼합하는 단계를 추가로 포함할 수 있다. In addition, the present invention may further include mixing the extract prepared in step (c) and the water-soluble extract prepared in step (d).
이때 상기 (c) 단계에서 제조된 추출물 및 상기 (d) 단계에서 제조된 수용성 추출물의 중량비는 60~80:20~40 인 것이 바람직하며, 상기 수치범위를 만족하는 경우 소취성, 노인냄새 제거특성, 항균성 및 항산화 특성이 극대화될 수 있다. At this time, the weight ratio of the extract prepared in step (c) and the water-soluble extract prepared in step (d) is preferably 60 to 80:20 to 40, and when the above numerical range is satisfied, deodorization and old odor removal characteristics , antibacterial and antioxidant properties can be maximized.
또한 본 발명은 상기 (c) 단계 이후에, 상기 제조된 추출물을 용매로 가열 추출하여 추출액을 제조하고, 상기 추출액을 감압 농축하여 추출물을 수득하는 단계를 추가로 포함할 수 있다. In addition, the present invention may further include, after step (c), heating and extracting the prepared extract with a solvent to prepare an extract, and concentrating the extract under reduced pressure to obtain an extract.
상기 가열 추출은 추출물 100중량부에 대하여 용매 100~2,000중량부를 가하고 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 가열하여 추출할 수 있다. 이때 추출액을 제조하는 단계에서 초음파를 인가할 수 있다. The heat extraction may be performed by adding 100 to 2,000 parts by weight of a solvent based on 100 parts by weight of the extract and heating at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa for 30 minutes to 10 hours. At this time, ultrasonic waves may be applied in the step of preparing the extract.
이때 용매로서 에탄올, 메탄올 및 물의 혼합용매를 사용하는 것이 바람직하다. 이때 상기 에탄올, 메탄올 및 물의 중량비는 30~60:20~40:100 인 것이 바람직하다. 에탄올, 메탄올 및 물의 중량비가 상기 수치범위를 만족하는 경우, 소취성, 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 극대화될 수 있다. At this time, it is preferable to use a mixed solvent of ethanol, methanol and water as a solvent. At this time, the weight ratio of the ethanol, methanol and water is preferably 30 to 60:20 to 40:100. When the weight ratio of ethanol, methanol and water satisfies the above numerical range, deodorization, old odor removal, yield, antibacterial and antioxidant properties can be maximized.
또한 본 발명은 상기 제조방법으로 제조되는 편백나무 잎 추출물에 관한 것이다. In addition, the present invention relates to a cypress leaf extract prepared by the above production method.
상기 편백나무 잎 추출물은 쉽고 간단하게 제조할 수 있으며, 소취성, 노인냄새 제거특성, 수득율, 항산화성, 항균성 등의 특성이 극대화될 수 있다. The cypress leaf extract can be easily and simply prepared, and properties such as deodorization, old odor removal, yield, antioxidant, and antibacterial properties can be maximized.
이하 실시예를 통해 본 발명을 상세히 설명한다. 하기 실시예는 본 발명의 실시를 위하여 예시된 것일 뿐, 본 발명의 내용이 하기 실시예에 의하여 한정되는 것은 아니다. The present invention will be described in detail through the following examples. The following examples are only exemplified for the practice of the present invention, and the content of the present invention is not limited by the following examples.
(실시예 1) 편백나무 잎 추출물의 제조 (Example 1) Preparation of cypress leaf extract
편백나무 잎을 채취하여 절단한 후, 흐르는 물에서 세척하였다. After collecting and cutting cypress leaves, they were washed in running water.
상기 세척된 편백나무 잎 100중량부에 대하여 용매 1,000중량부를 첨가한 후, 80℃의 온도 및 1MPa의 압력에서 3시간 가열 추출하여 추출액을 제조하였다. 용매로는 에탄올과 물의 혼합용매를 사용하였고, 에탄올과 물의 중량비를 조절하였다. After adding 1,000 parts by weight of a solvent based on 100 parts by weight of the washed cypress tree leaves, heat extraction was performed at a temperature of 80 ° C. and a pressure of 1 MPa for 3 hours to prepare an extract. A mixed solvent of ethanol and water was used as the solvent, and the weight ratio of ethanol and water was adjusted.
상기 추출액을 감압 농축하여 편백나무 잎 추출물을 수득하였다. The extract was concentrated under reduced pressure to obtain a cypress leaf extract.
(실시예 2) 편백나무 잎 추출물의 제조 (Example 2) Preparation of cypress leaf extract
편백나무 잎을 채취하여 절단한 후, 흐르는 물에서 세척하였다. After collecting and cutting cypress leaves, they were washed in running water.
상기 세척된 편백나무 잎 100중량부에 대하여 용매 1,000중량부를 첨가한 후, 80℃의 온도 및 1MPa의 압력에서 3시간 가열 추출하여 추출액을 제조하였다. 용매로는 에탄올, 메탄올 및 물의 혼합용매를 사용하였고, 에탄올, 메탄올 및 물의 중량비를 조절하였다. After adding 1,000 parts by weight of a solvent based on 100 parts by weight of the washed cypress tree leaves, heat extraction was performed at a temperature of 80 ° C. and a pressure of 1 MPa for 3 hours to prepare an extract. A mixed solvent of ethanol, methanol and water was used as the solvent, and the weight ratio of ethanol, methanol and water was adjusted.
상기 추출액을 감압 농축하여 편백나무 잎 추출물을 수득하였다. The extract was concentrated under reduced pressure to obtain a cypress leaf extract.
(실시예 3) 편백나무 잎 수용성 추출물의 제조 (Example 3) Preparation of cypress leaf water-soluble extract
편백나무 잎을 채취하여 절단한 후, 흐르는 물에서 세척하였다. After collecting and cutting cypress leaves, they were washed in running water.
상기 세척된 편백나무 잎 100중량부에 대하여 용매 1,000중량부를 첨가한 후, 80℃의 온도 및 1MPa의 압력에서 3시간 가열 추출하여 추출액을 제조하였다. 용매로는 에탄올과 물의 혼합용매를 사용하였고, 에탄올과 물의 중량비를 조절하였다. After adding 1,000 parts by weight of a solvent based on 100 parts by weight of the washed cypress tree leaves, heat extraction was performed at a temperature of 80 ° C. and a pressure of 1 MPa for 3 hours to prepare an extract. A mixed solvent of ethanol and water was used as the solvent, and the weight ratio of ethanol and water was adjusted.
상기 추출액을 감압 농축하여 편백나무 잎 추출물을 수득하였다. The extract was concentrated under reduced pressure to obtain a cypress leaf extract.
상기 제조된 편백나무 잎 추출물을 정제수로 수용화한 후, 감압 농축하여 정제수를 제거하여 편백나무 잎 수용성 추출물을 수득하였다. After water-solubilizing the prepared cypress leaf extract with purified water, it was concentrated under reduced pressure to remove the purified water to obtain a water-soluble extract of cypress tree leaf.
(실시예 4) 혼합 편백나무 잎 추출물의 제조 (Example 4) Preparation of mixed cypress leaf extract
실시예 1의 편백나무 잎 추출물 및 실시예 3의 편백나무 잎 수용성 추출물을 혼합하여 혼합 편백나무 잎 추출물을 제조하였다. A mixed cypress leaf extract was prepared by mixing the cypress leaf extract of Example 1 and the water-soluble extract of cypress leaf of Example 3.
상기 편백나무 잎 추출물 및 편백나무 잎 수용성 추출물 제조 시 사용되는 에탄올 및 물의 중량비는 40:60 이었다. The weight ratio of ethanol and water used in preparing the cypress cypress leaf extract and the cypress leaf water-soluble extract was 40:60.
(항균성) (antibacterial)
편백나무 잎 추출물에 대한 항균성은 ASTM E2149 SHAKE FLASK TEST로 측정하였다. 균(Staphylococcus aureus)이 배양된 배양액에 상기 추출물 100㎕을 넣고 24시간 배양 후 생존 균수를 측정한 후, 초기 균수 및 24시간 후 균수로부터 세균 감소율을 측정하였다. Antibacterial properties of cypress tree leaf extract were measured by ASTM E2149 SHAKE FLASK TEST. 100 μl of the extract was added to the culture medium in which the bacteria (Staphylococcus aureus) was cultured, and the number of viable bacteria was measured after incubation for 24 hours, and then the bacterial reduction rate was measured from the initial number of bacteria and the number of bacteria after 24 hours.
(항산화 특성) (antioxidant properties)
자유라디칼 소거활성 평가는 안정한 자유라디칼인 1,1-diphenyl-2-picrylhydrazyl radical (DPPH, Sigma, USA)을 이용하는 방법을 사용하였다. The free radical scavenging activity was evaluated using a stable free radical, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH, Sigma, USA).
테스트 튜브에 DPPH 용액, 시료 및 에탄올을 6:3:1의 부피비로 첨가한 후, 실온에서 10분 동안 방치한 다음 UV/Vis Spectrophotometer(OPTIZEN POP-S)로 517nm에서 흡광도를 측정하였다. After adding DPPH solution, sample, and ethanol in a volume ratio of 6:3:1 to a test tube, it was allowed to stand at room temperature for 10 minutes, and then absorbance was measured at 517 nm with a UV/Vis Spectrophotometer (OPTIZEN POP-S).
측정한 흡광도를 이용하여 각 추출물의 저해율(IR)을 아래의 식으로부터 계산하였으며, 측정된 저해율로부터 50% 저해활성 농도(IC50)를 결정하였다. The inhibition rate (IR) of each extract was calculated from the equation below using the measured absorbance, and the 50% inhibitory activity concentration (IC 50 ) was determined from the measured inhibition rate.
실시예 1로부터 제조된 추출물의 특성은 표 1에 제시된다. The properties of the extract prepared from Example 1 are presented in Table 1.
(%)yield
(%)
(%)bacterial reduction rate
(%)
(%)2-nonenal removal rate
(%)
(0:100)ethanol:water
(0:100)
(20:80)ethanol:water
(20:80)
(40:60)ethanol:water
(40:60)
(50:50)ethanol:water
(50:50)
(60:40)ethanol: water
(60:40)
(80:20)ethanol: water
(80:20)
(100:0)ethanol:water
(100:0)
상기 표에서 알 수 있듯이, 에탄올 및 물의 중량비가 15~45:55~85 인 경우, 추출물의 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 향상될 수 있다. 특히 에탄올 및 물의 중량비가 15~45:55~85 인 경우, 추출물의 노인냄새 제거특성 및 수득율이 현저히 향상될 수 있다. As can be seen from the table above, when the weight ratio of ethanol and water is 15 to 45:55 to 85, the extract's aging odor removal properties, yield, antibacterial and antioxidant properties can be improved. In particular, when the weight ratio of ethanol and water is 15 to 45:55 to 85, the aging odor removal characteristics and yield of the extract can be significantly improved.
도 1 및 도 2는 편백나무 잎 추출물의 항균 시험 결과 및 항산화 시험 결과를 나타낸다. 1 and 2 show antibacterial test results and antioxidant test results of cypress leaf extract.
GC-MS 분석 결과, 에탄올:물(0:100)로 추출한 추출물은 eudesmol 4.05%, inositol 12.69% 을 포함하고, 에탄올:물(20:80)로 추출한 추출물은 eudesmol 13.88%, inositol 41.45%, terpinen-4-ol 24.04% 을 포함하며, 에탄올:물(40:60)로 추출한 추출물은 eudesmol 10.67%, elemol 9.06%, inositol 33.63%, terpinen-4-ol 19.04% 을 포함하며, 에탄올:물(50:50)로 추출한 추출물은 eudesmol 7.44%, elemol 16.14%, inositol 12.24% 을 포함하며, 에탄올:물(60:40)로 추출한 추출물은 eudesmol 6.01%, elemol 2.95%, inositol 35.73%, terpinen-4-ol 0.92% 을 포함하며, 에탄올:물(80:20)로 추출한 추출물은 elemol 10.53%, inositol 18.69%, terpinen-4-ol 1.09% 을 포함할 수 있다. As a result of GC-MS analysis, the extract extracted with ethanol:water (0:100) contained eudesmol 4.05% and inositol 12.69%, and the extract extracted with ethanol:water (20:80) contained eudesmol 13.88%, inositol 41.45%, and terpinen. -4-ol 24.04%, and the extract extracted with ethanol:water (40:60) contains eudesmol 10.67%, elemol 9.06%, inositol 33.63%, terpinen-4-ol 19.04%, and ethanol:water (50 :50) contained eudesmol 7.44%, elemol 16.14%, and inositol 12.24%, and the extract extracted with ethanol:water (60:40) contained eudesmol 6.01%, elemol 2.95%, inositol 35.73%, and terpinen-4- ol 0.92%, and the extract extracted with ethanol:water (80:20) may contain elemol 10.53%, inositol 18.69%, and terpinen-4-ol 1.09%.
에탄올:물(20:80)로 추출한 추출물 및 에탄올:물(40:60)로 추출한 추출물은 높은 함량의 terpinen-4-ol을 포함하므로, 노인냄새 제거특성이 우수하다. Since the extract extracted with ethanol:water (20:80) and the extract extracted with ethanol:water (40:60) contain a high content of terpinen-4-ol, they have excellent aging odor removal properties.
또한 실시예 2로부터 제조된 추출물의 특성은 표 2에 제시된다. Also the properties of the extract prepared from Example 2 are presented in Table 2.
(%)yield
(%)
(%)bacterial reduction rate
(%)
(%)2-nonenal removal rate
(%)
(40:10:100)Ethanol:Methanol:Water
(40:10:100)
(40:30:100)Ethanol:Methanol:Water
(40:30:100)
(40:50:100)Ethanol:Methanol:Water
(40:50:100)
상기 표에서 알 수 있듯이, 에탄올, 메탄올 및 물의 중량비가 30~60:20~40:100 인 경우, 추출물의 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 향상될 수 있다. As can be seen from the above table, when the weight ratio of ethanol, methanol and water is 30 to 60:20 to 40:100, the old odor removal property, yield, antibacterial and antioxidant properties of the extract can be improved.
또한 실시예 3으로부터 제조된 추출물의 특성은 표 3에 제시된다. Also the properties of the extract prepared from Example 3 are presented in Table 3.
(%)yield
(%)
(%)bacterial reduction rate
(%)
(%)2-nonenal removal rate
(%)
(20:80)ethanol:water
(20:80)
(40:60)ethanol:water
(40:60)
(50:50)ethanol:water
(50:50)
상기 표에서 알 수 있듯이, 편백나무 잎 수용성 추출물의 경우, 편백나무 잎 추출물에 비해 추출물의 노인냄새 제거특성, 수득율, 항균성 및 항산화 특성이 향상됨을 알 수 있다. As can be seen from the table above, in the case of the water-soluble extract of cypress cypress, it can be seen that the old odor removal characteristic, yield, antibacterial and antioxidant properties of the extract are improved compared to the cypress leaf extract.
또한 실시예 4로부터 제조된 추출물의 특성은 표 4에 제시된다. Also the properties of the extract prepared from Example 4 are presented in Table 4.
(%)2-nonenal removal rate
(%)
(50:50)Cypress leaf extract: Cypress leaf water-soluble extract
(50:50)
(70:30)Cypress leaf extract: Cypress leaf water-soluble extract
(70:30)
(90:10)Cypress leaf extract: Cypress leaf water-soluble extract
(90:10)
상기 표에서 알 수 있듯이, 편백나무 잎 추출물 및 편백나무 잎 수용성 추출물의 중량비가 60~80:20~40 인 경우, 추출물의 노인냄새 제거특성 및 항산화 특성이 향상됨을 알 수 있다. As can be seen from the above table, when the weight ratio of the cypress tree leaf extract and the cypress tree leaf water-soluble extract is 60 to 80:20 to 40, it can be seen that the old odor removal properties and antioxidant properties of the extract are improved.
Claims (5)
(b) 상기 세척된 편백나무 잎 및 용매를 혼합하고, 40~150℃의 온도 및 0.5~10MPa의 압력에서 30분~10시간 열수 추출하여 추출액을 제조하는 단계; 및
(c) 상기 추출액을 감압 농축하여 추출물을 수득하는 단계를 포함하는 편백나무 잎 추출물의 제조방법.
(a) washing cypress leaves;
(b) preparing an extract by mixing the washed cypress leaves and a solvent and performing hot water extraction at a temperature of 40 to 150 ° C. and a pressure of 0.5 to 10 MPa for 30 minutes to 10 hours; and
(c) a method for producing a cypress leaf extract comprising the step of obtaining an extract by concentrating the extract under reduced pressure.
상기 단계 (b)는 초음파를 인가하여 추출액을 제조하는 것을 특징으로 하는 편백나무 잎 추출물의 제조방법.
According to claim 1,
The step (b) is a method for producing a cypress leaf extract, characterized in that for preparing an extract by applying ultrasonic waves.
상기 용매는 에탄올 및 물의 혼합용매인 것을 특징으로 하는 편백나무 잎 추출물의 제조방법.
According to claim 2,
The method for producing a cypress leaf extract, characterized in that the solvent is a mixed solvent of ethanol and water.
상기 에탄올 및 물의 중량비는 15~45:55~85 인 것을 특징으로 하는 편백나무 잎 추출물의 제조방법.
According to claim 3,
Method for producing a cypress leaf extract, characterized in that the weight ratio of the ethanol and water is 15 to 45: 55 to 85.
Cypress tree leaf extract prepared by the manufacturing method of claim 1.
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| KR20190070128A (en) * | 2017-12-12 | 2019-06-20 | 학교법인 송원대학교 | How to remove odor from the retinispora extract |
| KR102251854B1 (en) * | 2020-08-03 | 2021-05-13 | 최귀남 | Cosmetic composition for skin moisturizing and anti-wrinkle comprising Chamaecyparis obtusa leaf mixed extract as effective component |
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| KR20190031983A (en) | 2017-09-19 | 2019-03-27 | 신혜수 | Method for preparing extracts of Chamecyparis obutsa and a composition for life goods comprising the using the same |
| KR20190070128A (en) * | 2017-12-12 | 2019-06-20 | 학교법인 송원대학교 | How to remove odor from the retinispora extract |
| KR102251854B1 (en) * | 2020-08-03 | 2021-05-13 | 최귀남 | Cosmetic composition for skin moisturizing and anti-wrinkle comprising Chamaecyparis obtusa leaf mixed extract as effective component |
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