KR20200016101A - Method for producing the extracts of natural material using ethyl lactate - Google Patents

Abstract

A method for preparing a natural substance extract using ethyl lactate according to the present invention comprises the following steps: (a) obtaining a mixture by mixing an ethyl lactate solvent with a natural substance; (b) extracting an extract from the mixture; and (c) concentrating the extract to obtain a concentrated extract. The extraction yield of the extract from natural substances is excellent, the content of physiologically active substances in the extract can be increased, and an extract harmless to human body without toxicity can be obtained.

Description

Method for producing natural extract using ethyl lactate {METHOD FOR PRODUCING THE EXTRACTS OF NATURAL MATERIAL USING ETHYL LACTATE}

The present invention relates to a method for producing a natural substance extract using ethyl lactate, and to a method for producing a natural substance extract using ethyl lactate excellent in the extraction effect of the natural substance extract and harmless to the human body.

Food, cosmetics, pesticides and pharmaceutical companies extract extracts from natural natural substances such as plants, algae and microalgae. The extracted extract is used in products such as essential oils, pigments, medicinal substances, fragrances. In order to extract the extract from the natural substance, hot water extraction, supercritical extraction, solvent extraction is used. In addition, the solvent used for the extraction of the extract is used, such as hexane, ethyl acetate, methanol, water.

Since the solvent is in direct contact with the human body in a state dissolved in natural materials, it should not be harmful to humans. However, methanol solvent and hexane solvent are harmful to human body. In addition, ethyl acetate solvent and hexane solvent have a low yield of extract extraction from natural substances. In addition, the water solvent is excellent in the yield to extract the extract from the natural substance, but the type and extract amount of the extract extracted from the natural substance.

Therefore, it is possible to implement higher efficiency in extract extraction from natural substances, and there is still a need for research on solvents that are harmless to humans.

The present invention aims to provide a method for producing a natural substance extract using a novel solvent to solve the above problems.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problem, another task that is not mentioned will be clearly understood by those skilled in the art from the following description.

According to one embodiment of the present invention, the method for producing a natural substance extract using ethyl lactate according to the present invention comprises the steps of (a) obtaining a mixture by mixing an ethyl lactate solvent and a natural substance, (b) Extracting the extract from the mixture and (c) concentrating the extract to obtain a concentrated extract.

According to the present invention, a method for preparing a natural substance extract using ethyl lactate has an effect of increasing the extraction yield of the extract from the natural substance and increasing the content of a bioactive substance in the extract.

In addition, the method for producing a natural material extract using ethyl lactate according to the present invention has the effect of obtaining an extract that is harmless to humans and non-toxic by using ethyl lactate as a solvent.

The above objects, features, and advantages will be described in detail below, and therefore, those skilled in the art may easily implement the technical idea of the present invention. In describing the present invention, when it is determined that the detailed description of the known technology related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description will be omitted. Hereinafter, preferred embodiments of the present invention will be described in detail.

The present invention is not limited to the embodiments disclosed below, but can be implemented in various different forms, only this embodiment to make the disclosure of the present invention complete and completely to the person skilled in the art the scope of the invention It is provided to inform you.

<Ethyl Lactate  Manufacturing Method of Natural Material Extract Using>

The method for producing a natural substance extract using ethyl lactate according to the present invention comprises the steps of: (a) mixing an ethyl lactate solvent and a natural substance to obtain a mixture, (b) extracting an extract from the mixture, and (c) concentrating the extract to obtain a concentrated extract to prepare a natural substance extract.

First, the preparation method according to the present invention includes the steps of (a) mixing an ethyl lactate solvent and a natural material to obtain a mixture.

The preparation method according to the present invention uses ethyl lactate as a solvent.

The IUPAC name for ethyl lactate is an ester derivative formed from lactic acid and ethanol as Ethyl-2-hydroxypropanoate. The ethyl lactate has a hydroxyl group (-OH) on carbon 2 It has a structure of following General formula (1) specifically ,.

[Formula 1]

The ethyl lactate is contained in various foods such as wine and chicken, and is present as a liquid at -26 ° C to 154 ° C. The ethyl lactate may be used as a solvent, and the ethyl lactate solvent is colorless and transparent in appearance.

The ethyl lactate solvent is not toxic unlike conventional methanol solvents and hexane solvents. The ethyl lactate solvent is also fully biodegradable. In addition, the ethyl lactate solvent is a protic polar solvent containing an -OH group in the molecule of ethyl lactate and can be completely dissolved in water, alcohol, and the like. In addition, the ethyl lactate solvent has an excellent yield in extracting the extract from natural substances. In addition, the ethyl lactate solvent has an effect of increasing the type of the extract and the amount of the extract in extracting the extract from the natural substance.

The natural substance of step (a) is a substance existing in nature as a plant, such as a herbal medicine. Specifically, the natural materials of the step (a) are health (ginger), tobacco leaves, Angelica, rhubarb, machi, mammundong, cornus, baekbaekpi, love leaf, root root, ginseng, peony, red sesame, cheongung, astragalus, hwangchil leaves, licorice , Peppermint, pyrethrum, paw paw leaves, and the like. Preferably, the natural material of step (a) may include one or more of rhubarb, peony, cheonggung, tangwi, and ginseng.

Preferably, step (a) may be performed by mixing the ethyl lactate solvent and the natural material in a volume ratio of 5: 1 to 100: 1. More preferably, the volume ratio of the ethyl lactate solvent and the natural material may range from 15: 1 to 25: 1. When the volume ratio of the ethyl lactate solvent / natural material is less than 5, the yield of extracting the extract from the natural material may be lowered. In addition, when the volume ratio of the ethyl lactate solvent / natural material exceeds 100, the efficiency of extracting the extract from the natural material may be reduced.

Next, the production method according to the present invention includes the step of (b) extracting the extract from the mixture.

The step (b) may be a step of uniformly mixing the natural material in the ethyl lactate solvent by stirring the mixture, extracting the extract for a predetermined temperature and time.

Preferably, the extraction process in step (b) may be performed for 1 to 24 hours at a temperature range of 20 ℃ to 200 ℃. More preferably, the extraction process in step (b) may be performed for 2 to 5 hours at a temperature range of 60 ℃ to 180 ℃. When the temperature range for extracting the extract is less than 20 ℃ yield may be reduced to extract the extract from the natural material. In addition, when the temperature range for extracting the extract exceeds 200 ℃, the kind and amount of the extract extracted from the natural material may be lowered.

Next, the manufacturing method according to the present invention includes the step of obtaining the concentrated extract (c) by concentrating the extract.

In the step (c), the extract is concentrated for a predetermined time to remove a considerable amount of the ethyl lactate solvent present in the extract, to obtain a concentrated extract from the extract.

Preferably, the step (c) may be carried out a process of concentrating the extract after filtering the precipitates and impurities present in the extract. The concentration process of the extract may be a known concentration process may be used and preferably may be carried out using a vacuum pressure concentrator. By the concentration process, the ethyl lactate solvent included in the extract may be removed in substantial amounts. Accordingly, in the step (c), it is possible to obtain a concentrated extract from which precipitates, impurities, and ethyl lactate solvent are removed, and natural extract is obtained from the concentrated extract.

Next, the manufacturing method according to the present invention may further include (d) drying the concentrated extract.

The remaining amount of the ethyl lactate solvent present in the concentrated extract may be removed by step (d). Preferably, in step (d), a freeze-drying process may be performed to minimize the damage of the concentrated extract. Natural material extract obtained by the freeze-drying process has the effect of increasing the shelf life.

The natural material extract prepared according to the production method of the present invention may include one or more components of polyphenols and flavonoids. The polyphenol is a chemical found in plants and has a sulfated effect of removing active oxygen in the human body. In addition, the polyphenol has the effect of protecting DNA, cellular proteins and enzymes in the human body. In addition, the polyphenol has an anticancer effect and prevents heart disease. The flavonoids are chemicals found in plants such as polyphenols and have biological and pharmacological effects such as anti-allergic and anti-inflammatory properties.

In addition, the natural substance extract may exhibit DPPH (1-diphenyl-2-picryl hydrazyl) radical scavenging activity. The DPPH is a kind of free radicals, and the extract of natural substances has an effect of eliminating DPPH free radicals, which are harmful substances in the human body. In addition, the natural substance extract has an antioxidant activity effect that effectively removes free radicals in the human body by eliminating DPPH radicals in the human body.

Hereinafter, specific embodiments of the present invention will be described through examples.

< Example >

<Example 1>

Rhubarb, Peony, Cheongung, Angelica, Ginseng, Peppermint, Pyrethrum, and Popo Leaves were pulverized, stirred with ethyl lactate (100%) solvent, and extracted at 80 ° C. for 3 hours. Thereafter, the extract was filtered and concentrated by vacuum concentrator to obtain a concentrated extract. Thereafter, the concentrated extract was lyophilized to obtain a natural substance extract.

<Example 2>

Rhubarb, Peony, Cheongung, Dongguk, Ginseng, Peppermint, Pyrethrum, and Popo Leaves were pulverized and stirred with ethyl lactate (100%) solvent and extracted at 150 ° C.

Comparative Example 1

It is the same as Example 1 except using methanol (MeOH) as a solvent and extracted at 65 ℃.

Comparative Example 2

It is the same as Example 1 except using hexane (Hexane) as a solvent and extracted at 65 ℃.

Comparative Example 3

It was the same as Example 1 except using ethyl acetate as a solvent and extracting at 75 ℃.

<Comparative Example 4>

Is the same as in Example 1 except for using the water (H ₂ O) as the solvent and extracted from 100 ℃.

Experimental Example

1. Determination of Polyphenol Content

In order to confirm the polyphenol content of each sample according to the solvents of Examples 1 and 2 and Comparative Examples 1 to 4, 400 μL of an extract dissolved in methanol at a concentration of 1 mg / mL and 400 μL of Folin-Denis reagent, 10% Na ₂ 400 μL CO ₃ was mixed. After the reaction mixture for 50 minutes in the dark and centrifuged for 5 minutes at 13,000rpm using a centrifuge. Thereafter, 120 μL of the supernatant in the mixture was dispensed on a 96-well plate, and the absorbance was measured at 700 nm. A calibration curve was prepared using tannic acid as a standard, and the content of the phenolic compound of each extract was shown in Table 1 as mg / g tannic acid equivalent (TAE).

2. Determination of Flavonoid Content

In order to confirm the flavonoid content of each sample according to the solvents of Examples 1 and 2 and Comparative Examples 1 to 4, 100 μL of extract dissolved in methanol at a concentration of 1 mg / mL, 20 μL of 10% aluminum nitrate, 20 μL of 1M potassium acetate, 860 μL of methanol was mixed. Thereafter, the mixture was reacted for 40 minutes, and 100 μL was dispensed on a 96-well plate, and the absorbance was measured at 420 nm. A calibration curve was prepared using rutin as a standard, and the flavonoid content of each extract was shown in Table 2 as mg / g rutin equivalent (RE).

3. Evaluation of DPPH Radical Scavenging Activity

In order to evaluate the DPPH radical scavenging activity for each sample according to the solvents of Examples 1 and 2 and Comparative Examples 1 to 4, 20 μL of the extract diluted in methanol was dispensed in a 96well plate, and 200 μM DPPH (1-diphenyl-2-picryl 180 μL of a hydrazyl) solution was added thereto, followed by reaction in the dark for 15 minutes, and the absorbance was measured at 517 nm. Based on the results, an SC ₅₀ value representing the concentration of the extract needed to eliminate 50% of the DPPH radicals was calculated. In addition, Relative activity (%) was calculated by comparing the SC ₅₀ value of each extract based on the SC ₅₀ value of ascorbic acid (vitamin C) as a control. Tables 3 and 4 show the SC ₅₀ and Relative activity (%) of the extract, respectively. The lower the SC ₅₀ value and the higher the relative activity (%) value, the better the DPPH radical scavenging activity.

4. Evaluation of Extraction Yield by Sample

In order to confirm the extraction yield for each sample according to the solvents of Examples 1,2 and Comparative Examples 1 to 4, the yield (%) was measured by the weight of the extract (g) / the weight of the sample (g) x 100 [Table] 5].

Polyphenol Content by Sample

Polyphenol Content (mg / g (TAE)) rhubarb Peony Cheongung Donkey Ginseng mint Pyrethrum papaw
leaf Example 1 208.70 63.70 21.07 23.90 21.00 - - - Example 2 139.80 73.50 29.11 27.90 50.20 - - - Comparative Example 1 7.30 97.40 20.36 7.30 10.10 - - - Comparative Example 2 29.60 15.20 9.89 7.40 1.10 - - - Comparative Example 3 217.70 33.10 18.30 10.80 3.90 - - - Comparative Example 4 7.60 68.30 7.80 7.60 3.60 - - -

Looking at the results of Table 1, the content of polyphenol extracted using ethyl lactate solvent from Cheongung, Angelica, and Ginseng was poly-extracted using methanol solvent, hexane solvent, ethyl acetate solvent, and water from Cheongung, Angelica, Ginseng It can be seen that it is higher than the content of phenol. In particular, it can be seen that the content of polyphenols extracted using ethyl lactate solvent at 150 ° C. from Cheongung, Angelica and Ginseng was the highest.

 Flavonoid Content by Sample

Flavonoid Content (mg / g (RE)) rhubarb Peony Cheongung Donkey Ginseng mint Pyrethrum papaw
leaf Example 1 4.2 0.7 1.23 0.4 0.1 - - - Example 2 0.0 1.7 1.85 0.8 0.5 - - - Comparative Example 1 3.5 0.3 1.54 0.0 0.8 - - - Comparative Example 2 0.4 -0.2 0.44 3.2 11.8 - - - Comparative Example 3 -3.7 0.0 0.93 0.5 11.9 - - - Comparative Example 4 0.0 -0.3 2.55 0.0 -1.6 - - -

Looking at the results of Table 2 it can be seen that the content of the flavonoids extracted with ethyl lactate solvent from rhubarb, peony, in particular, is higher than the flavonoids extracted with the solvent used in the comparative example.

DPPH radical scavenging activity by sample-SC ₅₀

SC ₅₀ (mg / mL) rhubarb Peony Cheongung Donkey Ginseng mint Pyrethrum papaw
leaf Example 1 0.316 0.974 7.321 11.07 9.487 0.503 0.799 2.138 Example 2 0.649 1.621 8.069 9.145 4.908 0.590 0.881 0.305 Comparative Example 1 0.302 1.081 7.413 16.47 23.62 0.924 1.105 1.762 Comparative Example 2 5.00 15.36 6.316 22.80 141.5 48.02 14.55 7.449 Comparative Example 3 0.176 0.987 4.080 13.77 29.53 3.904 6.509 8.643 Comparative Example 4 0.959 0.777 11.34 15.29 25.48 0.921 1.345 2.892 Vitamin C (Control) 0.054 0.105 0.184 0.105 0.184 0.076 0.201 0.201

 DPPH radical scavenging activity by sample-Relative activity

Relative activity (%) rhubarb Peony Cheongung Donkey Ginseng mint Pyrethrum papaw
leaf Example 1 17.10 10.78 2.51 0.95 0.75 39.97 25.03 9.40 Example 2 8.32 6.48 2.28 1.15 1.45 34.06 22.70 65.82 Comparative Example 1 17.89 13.51 2.48 0.64 0.30 21.77 19.70 11.41 Comparative Example 2 1.08 0.68 2.91 0.46 0.05 0.42 1.37 2.70 Comparative Example 3 30.66 10.64 4.51 0.76 0.24 5.15 3.07 2.33 Comparative Example 4 5.63 9.71 1.62 0.69 0.28 21.82 14.87 6.95 Vitamin C (Control) 100 100 100 100 100 100 100 100

Looking at the results of Table 3, Table 4 it can be seen that the content of antioxidants extracted using ethyl lactate solvent, especially from Angelica, ginseng, peppermint, wormwood, paw paw leaves.

Extraction yield by sample

Extraction yield (%) rhubarb Peony Cheongung Donkey Ginseng mint Pyrethrum papaw
leaf Example 1 17.15 5.72 11.11 17.96 4.38 2.45 5.31 5.47 Example 2 44.20 27.57 33.26 6.65 8.63 6.80 6.37 11.58 Comparative Example 1 27.16 18.50 22.90 29.27 12.97 16.70 16.40 14.73 Comparative Example 2 0.43 0.30 3.33 1.13 0.97 2.40 2.83 3.02 Comparative Example 3 4.08 0.79 4.60 1.57 1.08 3.71 4.04 5.37 Comparative Example 4 43.60 25.86 29.02 68.96 7.30 28.06 20.26 19.14

Looking at the results of Table 5 it can be seen that the extraction yield compared to hexane solvent, ethyl acetate solvent especially when extracted using rhubarb, peony, cheongung using an ethyl lactate solvent.

 As described above, the extraction of natural materials using ethyl lactate solvent can be seen that the yield of the extraction of natural materials is superior to the extraction of natural materials using natural hexane solvent and ethyl acetate solvent. In addition, the extraction of natural materials using ethyl lactate solvent can be seen that the efficiency of extracting the polyphenol components compared to methanol solvent, hexane solvent, ethyl acetate solvent and water. In addition, the extraction of natural substances using ethyl lactate solvent can be seen that the efficiency of extracting the flavonoid components compared to methanol solvent and water. In addition, it can be seen that the natural substance extract extracted from phosphoric acid, peppermint, P. pylori, and paw paw leaves using ethyl lactate solvent has a high content of antioxidant activity.

Although the present invention has been described as described above, the present invention is not limited to the embodiments disclosed herein, and it is obvious that various modifications can be made by those skilled in the art within the scope of the technical idea of the present invention. In addition, even if the above described embodiments of the present invention while not explicitly described and described the effect of the effect of the configuration of the present invention, it is obvious that the effect predictable by the configuration is also to be recognized.

Claims (7)

(a) mixing an ethyl lactate solvent with a natural substance to obtain a mixture;
(b) extracting the extract from the mixture; And
(c) concentrating the extract to obtain a concentrated extract; to prepare a natural substance extract, including
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
After step (c),
(d) drying the concentrated extract;
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
The natural extract is characterized in that it comprises at least one component of polyphenol (polyphenol) and flavonoids (flavonoid)
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
The natural material is characterized in that it comprises one or more of rhubarb, peony, cheonggung, tangwi and ginseng
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
The natural substance extract is characterized in that it has a DPPH (1-diphenyl-2-picryl hydrazyl) radical scavenging activity
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
In step (a),
The ethyl lactate solvent and the natural material are characterized in that the volume ratio is mixed in the range of 5: 1 to 100: 1.
Method for preparing natural substance extract using ethyl lactate.
The method of claim 1,
Step (b),
The mixture is extracted for 1 to 24 hours at 20 ℃ to 200 ℃
Method for preparing natural substance extract using ethyl lactate.