CN103636406B - Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers - Google Patents
Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers Download PDFInfo
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- CN103636406B CN103636406B CN201310660445.8A CN201310660445A CN103636406B CN 103636406 B CN103636406 B CN 103636406B CN 201310660445 A CN201310660445 A CN 201310660445A CN 103636406 B CN103636406 B CN 103636406B
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Abstract
The invention discloses a method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers. The method comprises the following steps that firstly, corn flour and wheat flour are mixed, water is added to the mixed flour, paste is formed in a boiled mode, and the paste is cooled for standby application; secondly, the sponge is placed in the paste and stirred repeatedly, so that the paste permeates into holes of the sponge, then the sponge is pulled out, superfluous paste is squeezed, the sponge is placed in dry bran to be stirred and rolled over, and the bran adheres to the surfaces of the sponge; thirdly, the sponge with the bran in an adhering mode is placed in double-layer polypropylene bags uniformly, the surfaces are covered with fine wood chips, and sealing is conducted; fourthly, sterilization is conducted, inoculation is conducted after cooling, and cultivation is conducted. The method for manufacturing the edible fungus spawn is conducted by using the polyurethane sponge as the spawn carriers, the finished spawn is attached to the sponge after being cultivated, and the finished spawn can be processed into any physical shapes in an industrial and standardized mode; the finished spawn is attached to the sponge carriers, the finished spawn is easily separated from the sponge in a using process and is easily scattered in a pounded mode, and it can be avoided that the vitality of the spawn is damaged in an inoculation process because the spawn is bounded to pieces.
Description
Technical field
The present invention relates to a kind of preparation method of Edible Fungi bacterial classification, be specifically related to a kind of polyurethane sponge that utilizes as the preparation method of the edible fungus species of bacterial classification carrier.
Background technology
Edible fungus species is the seed of Edible Fungi, is the starting point of Edible Fungi.Select different materials to make edible fungus species and relate to the vigor of bacterial classification, quantity and use and keeping property.The present invention utilizes sponge and part grain class raw material to not only simplify production of hybrid seeds operation to make edible fungus species, substantially increases the serviceability of bacterial classification, has widened the production region of bacterial classification, increased work efficiency.
At present, edible fungus solid spawn divides by material two kinds, and one is timber, forage is the theme adds a small amount of grain, and one directly all uses grain (wheat berry, iblet or paddy), and manufacture craft is basically identical.Traditional edible fungus species all selects living beings as material, and after simple process and processing, make bacterial classification, the physical aspect of bacterial classification is limited by the natural attribute of material substantially.
Traditional edible fungus species all selects living beings as material, and after simple process or processing, make bacterial classification, the physical aspect of bacterial classification is limited by the natural attribute of material substantially, or needs very high processing cost just can meet instructions for use.And, finished product bacterial classification is in use not easy to operation, inoculum concentration cannot be accomplished unanimously homogeneous, thus be difficult to accomplish regulation and standardization, in seeded process, also can injure spawn activity because smashing bacterial classification to pieces, manufacturing process is time-consuming simultaneously, cost is higher.
Summary of the invention
The object of this invention is to provide a kind of polyurethane sponge that utilizes as the preparation method of the edible fungus species of bacterial classification carrier, the method is using polyurethane sponge as carrier, and finished product bacterial classification can on sponge, not need in use procedure to smash to pieces by appendix uniformly.
In order to reach above-mentioned technique effect, the present invention takes following technical scheme:
Utilize polyurethane sponge as a preparation method for the edible fungus species of bacterial classification carrier, comprise the following steps:
Step one: by corn flour and wheat flour mixing, add water and be brewed into paste, cool for subsequent use;
Step 2: sponge is put into paste and repeatedly stirs, makes paste infiltrate in sponge hole, then pulls the paste that extruding is unnecessary out, then puts into dry wheat bran stirring rolling, makes sponge surface adhere to wheat bran;
Step 3: the sponge adhering to wheat bran is evenly loaded double-deck Polypropylene Bag, the thin wood chip of surface coverage, sealing;
Step 4: sterilizing, inoculation after cooling, cultivates.
Further technical scheme is: described corn flour and the mass ratio of wheat flour are: (2 ~ 5): 1; The particle diameter of corn flour is below 40 orders.
Further technical scheme is: the mass fraction of described paste is 20 ~ 30%.
Further technical scheme is: the polyurethane porous sponge of described sponge to be perforate be 1 ~ 3mm.
Further technical scheme is: the shape of described sponge is spherical, disk, square or square bar, can according to user demand, and sponge can also be arbitrary shape.
Further technical scheme is: described sterilising temp is 123 DEG C, and sterilization time is 180min.
The present invention compared with prior art, has following beneficial effect:
(1) preparation method of this bacterial classification adopts sponge as carrier, after finished product breeding strain, appendix is on sponge, industrialized standard finished product bacterial classification can be processed into any physical shape, finished product bacterial classification appendix is on sponge carrier, in use finished product bacterial classification is very easily separated with sponge, very easily smashing loose, in seeded process, spawn activity can not be injured because smashing to pieces.
(2) adopt sponge as carrier, corn flour, wheat flour and wheat bran are as nutrient source, and the bacterial classification water binding capacity of cultivation, good air permeability, mycelial growth is healthy and strong rapidly.
(3) the uniform appendix of finished product bacterial classification of the present invention's cultivation is on sponge carrier, can reach inoculum concentration unanimously homogeneous during inoculation, accomplishes standardization and standardization.
(4) preparation method of this bacterial classification is applicable to the breeding strain of different mushroom; Meanwhile, the material source in this method extensively, is not subject to the restrictions such as season, region and material.
Embodiment
Below in conjunction with embodiments of the invention, the invention will be further elaborated.
Embodiment 1:
The preparation method of pleurotus eryngii quel strains:
Step 1: material and formula
Corn flour 800g, wheat flour 200g, porous sponge blockage 20mm × 20mm × 150mm500g, dry wheat bran is appropriate, and wooden branch 170mm × 7mm × 7mm(soaks 23 hours in 1 hour again with 3% lime poach in advance).Xingbao mushroom produces general needs in the punching of bacterium bag SMIS, thus during inoculation, bacterial classification is accessed bacterium bag bottom, to ensure that mycelia grows from upper, middle and lower simultaneously, uses the object of branch to be convenient to inoculation operation.
Step 2: paste makes
The corn flour prepared, wheat flour are joined in 3 liters of cold running waters and stir, is heated to boiling and boils 20 minutes, be brewed into paste, cool for subsequent use.
Step 3: wrap up in material
Sponge block is mixed in paste and repeatedly stirs, make paste infiltrate sponge hole completely, then pull extruding out, the extruding of unnecessary paste is removed.Be poured in dry wheat bran and stir rolling, make sponge block surface adhesion wheat bran; Wood branch is pulled out after adopting limewash to soak and is used clear water shower one time, repeatedly turns and make it fully to adhere to wheat bran in excessive dry wheat bran.
Step 4: dress bag
At 17cm × 35cm × 5s(5s=0.05mm) Polypropylene Bag in side first load 37 branches, again the sponge block wrapping material is evenly loaded 37, the thin wood chip of surface coverage one deck 2cm thickness, SMIS inserts long 180mm diameter 22mm sticking plaster one, the 3.3cm bore loudspeaker collar and supporting non-woven fabrics lid seal.It is to original seed block be accessed bacterial classification bag SMIS bottom when inoculating, guaranteeing that mycelia grows from upper, middle and lower simultaneously that SMIS inserts sticking plaster, shortens the breeding strain cycle.
Step 5: sterilizing
Rear dress basket of having packed pushes pressure cooker sterilizing, sterilising temp 123 DEG C, sterilization time 180min.
Step 6: cooling inoculation and cultivation
Pull out the sticking plaster access original seed in bag when being cooled to bag core temperature less than 28 DEG C after sterilizing terminates, then proceed to 23 ~ 25 DEG C, breeding strain storehouse dark and cultivate, until mycelia covers with bacterium bag.
Step 7: finished product
After mycelia is covered with, reject bacteria infection and have damaged strain bag, that selects vigorous, the pure white stalwartness of mycelial growth is finished product bacterial classification.
In 26 days Spawn incubation cycles, the dense stalwartness of mycelia, during use, sponge bacterial classification block is very easily smash loose, inserts a branch rear surface and accesses one piece of sponge bacterial classification block, smash loose capping kind without the need to effort during inoculation Xingbao mushroom bacterium bag.24 hours actication of culture time, the bacterial classification made than conventional method fast 12 little time, inoculum concentration stable homogeneous, handled easily, effect fast 1/3rd.
Embodiment 2:
The preparation method of agrocybe bacterial classification:
Step 1: material and formula
Corn flour 800g, wheat flour 200g, porous sponge blockage 40mm × 40mm × 150mm300g, dry wheat bran is appropriate.
Step 2: paste makes
The corn flour prepared, wheat flour are joined in 3 liters of cold running waters and stir, is heated to boiling and boils 20 minutes, be brewed into paste, cool for subsequent use.
Step 3: wrap up in material
Sponge block is mixed in paste and repeatedly stirs, make paste infiltrate sponge hole completely, then pull extruding out, the extruding of unnecessary paste is removed.Be poured in dry wheat bran and stir rolling, make sponge block surface adhesion wheat bran.
Step 4: dress bag
At 15cm × 33cm × 5s(5s=0.05mm) Polypropylene Bag in the sponge block wrapping material is evenly loaded to work loading height about 180mm, the thin wood chip of surface coverage one deck 2cm thickness, the 3.3cm bore loudspeaker collar and supporting non-woven fabrics lid seal.
Step 5: sterilizing
Rear dress basket of having packed pushes pressure cooker sterilizing, and sterilising temp is 123 DEG C, and sterilization time is 180min.
Step 6: cooling inoculation and cultivation
Access original seed when being cooled to bag core temperature less than 28 DEG C after sterilizing terminates, then proceed to 23 ~ 25 DEG C, breeding strain storehouse dark and cultivate, until mycelia covers with bacterium bag.
Step 7: finished product
After mycelia is covered with, reject bacteria infection and have damaged strain bag, that selects vigorous, the pure white stalwartness of mycelial growth is finished product bacterial classification.
In 20 days Spawn incubation cycles, the dense stalwartness of mycelia, during use, sponge bacterial classification block is very easily smash loose, and during inoculation agrocybe bacterium bag, surface access one piece of sponge bacterial classification block, smashes loose capping kind without the need to effort.24 hours actication of culture time, the bacterial classification made than conventional method fast 24 little time, inoculum concentration stable homogeneous, handled easily, effect is fast again.
Although with reference to explanatory embodiment of the present invention, invention has been described here, above-described embodiment is only the present invention's preferably embodiment, embodiments of the present invention are not restricted to the described embodiments, should be appreciated that, those skilled in the art can design a lot of other amendment and embodiment, these amendments and embodiment will drop within spirit disclosed in the present application and spirit.
Claims (5)
1. utilize polyurethane sponge as a preparation method for the edible fungus species of bacterial classification carrier, it is characterized in that comprising the following steps:
Step one: by corn flour and wheat flour mixing, add water and be brewed into the paste of mass fraction 20 ~ 30%, cool for subsequent use;
Step 2: sponge is put into paste and repeatedly stirs, makes paste infiltrate in sponge hole, then pulls the paste that extruding is unnecessary out, then puts into dry wheat bran stirring rolling, makes sponge surface adhere to wheat bran;
Step 3: the sponge adhering to wheat bran is evenly loaded double-deck Polypropylene Bag, the thin wood chip of surface coverage, sealing;
Step 4: sterilizing, inoculation after cooling, cultivates.
2. the polyurethane sponge that utilizes according to claim 1 is as the preparation method of the edible fungus species of bacterial classification carrier, it is characterized in that the mass ratio of described corn flour and wheat flour is: (2 ~ 5): 1; The particle diameter of corn flour is below 40 orders.
3. the polyurethane sponge that utilizes according to claim 1 is as the preparation method of the edible fungus species of bacterial classification carrier, it is characterized in that described sponge to be perforate be the polyurethane porous sponge of 1 ~ 3mm.
4. the polyurethane sponge that utilizes according to claim 1 is as the preparation method of the edible fungus species of bacterial classification carrier, it is characterized in that the shape of described sponge is spherical, disk, square or square bar.
5. the polyurethane sponge that utilizes according to claim 1 is as the preparation method of the edible fungus species of bacterial classification carrier, and it is characterized in that described sterilising temp is 123 DEG C, sterilization time is 180min.
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| CN201310660445.8A CN103636406B (en) | 2013-12-05 | 2013-12-05 | Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers |
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| CN201310660445.8A CN103636406B (en) | 2013-12-05 | 2013-12-05 | Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers |
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| CN103636406A CN103636406A (en) | 2014-03-19 |
| CN103636406B true CN103636406B (en) | 2015-03-11 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5634243B2 (en) * | 1978-11-29 | 1981-08-08 | ||
| JPS63109724A (en) * | 1986-10-29 | 1988-05-14 | 宮脇 彬行 | Culture of shiitake |
| JPH01181729A (en) * | 1988-01-13 | 1989-07-19 | Kanebo Ltd | Medium for mushroom seed fungus |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| JPH0416123A (en) * | 1990-05-09 | 1992-01-21 | House Food Ind Co Ltd | Medium for mushroom |
| JP2001128547A (en) * | 1999-11-02 | 2001-05-15 | Yasutoshi Sato | Mushroom culture with kenaf, a plant originating from africa |
| CN2452269Y (en) * | 1999-08-27 | 2001-10-10 | 邹书海 | Cultivation material bag for edible mushrooms |
| JP2002204685A (en) * | 2001-01-09 | 2002-07-23 | Yoneya Kk | Culture medium for cultivating filamentous fungus |
| KR20060010454A (en) * | 2004-07-28 | 2006-02-02 | 한상노 | Method of human work cultivation by using high density of phellinus linteus |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100232710B1 (en) * | 1997-08-05 | 1999-12-01 | 이재일 | A cultivating method for cordyceps militaris |
-
2013
- 2013-12-05 CN CN201310660445.8A patent/CN103636406B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5634243B2 (en) * | 1978-11-29 | 1981-08-08 | ||
| JPS63109724A (en) * | 1986-10-29 | 1988-05-14 | 宮脇 彬行 | Culture of shiitake |
| JPH01181729A (en) * | 1988-01-13 | 1989-07-19 | Kanebo Ltd | Medium for mushroom seed fungus |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| JPH0416123A (en) * | 1990-05-09 | 1992-01-21 | House Food Ind Co Ltd | Medium for mushroom |
| CN2452269Y (en) * | 1999-08-27 | 2001-10-10 | 邹书海 | Cultivation material bag for edible mushrooms |
| JP2001128547A (en) * | 1999-11-02 | 2001-05-15 | Yasutoshi Sato | Mushroom culture with kenaf, a plant originating from africa |
| JP2002204685A (en) * | 2001-01-09 | 2002-07-23 | Yoneya Kk | Culture medium for cultivating filamentous fungus |
| KR20060010454A (en) * | 2004-07-28 | 2006-02-02 | 한상노 | Method of human work cultivation by using high density of phellinus linteus |
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| CN103636406A (en) | 2014-03-19 |
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Address after: 611700 five groups of Zhan village, Tang Chang town, PI Du District, Chengdu, Sichuan Patentee after: Chengdu Yan Rongzhen industry limited company Address before: 610000 group 5, Zhan Qi Village, Tang Chang town, Pixian, Chengdu, Sichuan Patentee before: Chengdu Rongzhen Mushroom Industry Co.,Ltd. |