JP2002204685A - Culture medium for cultivating filamentous fungus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To utilize bean jam refuse having been discarded so far without being substantially put to reuse. SOLUTION: This culture medium for cultivating filamentous fungi is obtained by the following procedure: bean jam refuse is used as the main feedstock; the bean jam refuse is incorporated and intimately mixed with an organic nutrient auxiliary such as rice bran, an inorganic nutrient auxiliary such as potassium and a culture auxiliary such as sponge; since almost all of these auxiliaries are poor in economical usefulness as said to be so called wastes, they are usable inexpensively; both the pH and moisture content of the resultant mixture are adjusted to obtain the objective culture medium for proliferating filamentous fungi therein. Filamentous fungi proliferated in the above medium are pure and can easily be dried or put to mixing processing with other component(s) without being contaminated with other kinds of fungi and also without separation from culture residues.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture medium for filamentous fungi using an anesed cake as a main raw material.

[0002]

[Prior art] Raw bean paste is the main raw material for various sweets and yokans, including red beans, bamboo red beans, Tohoku red beans, Tianjin red beans, Taisho Kinto beans,
After processing quail beans, kidney beans, peas, hand beans, and other beans, a large amount of food processing residues are extracted after removing the bean jam particles contained in the beans. It has a high water content, is poor in taste, and is susceptible to spoilage and is easily degraded. It is taken free of charge by livestock farmers,
At present, most waste is paid for as industrial waste.

On the other hand, in the fields of food, medicine, agriculture, etc. in Japan, products using various filamentous fungi are actively made, but the filamentous fungi used for these are extremely limited and strictly regulated by specialized manufacturers. Produced and sold by pure culture under good control. However, in the field of agriculture, a large amount of filamentous fungi must be used, so it is necessary to obtain them at a low price, but it is difficult in practice.

Therefore, in the agricultural field, generally,
When manufacturing organic fertilizers and composts, some of the ripe organic fertilizers and composts are returned to organic matter such as straw, brassica, and fallen leaves as an inoculum for cells, and cultured for generations to continue production. However, the production of enzymes, vitamins, antibiotics, amino acids, sugars, plant hormones, etc., which must be exhibited due to the mutation of filamentous fungi, and the ability to control crop disease fungi are extremely unstable. However, in some cases, there is no effect at all, so that it greatly affects the generation and growth of other microorganisms, and at present, desired organic fertilizers and organic composts have not been obtained.

[0005]

DISCLOSURE OF THE INVENTION The present invention is intended to effectively use bean jam which has been discarded without being used so far, and to effectively use this as a solid culture medium for filamentous fungi. It is an object of the present invention to provide a medium capable of pure culture and production of cell cultures such as spores without the need for strict culture control, in a wide range of fields including agriculture, in large quantities, and at low cost.

[0006]

Means for Solving the Problems In the present invention, bean jam is used as a main material, and organic nutrients such as rice bran, inorganic nutrients such as potassium, and cultivation aids such as sponge are mostly added thereto. It mainly uses economically ineffective materials such as so-called wastes, and adjusts the pH and moisture thereof to economically obtain a culture medium for growing filamentous fungi. The filamentous fungus grown in this culture medium is pure without any other fungi, and without separation of the culture residue and cells, and can be easily dried and mixed with other components. It can be carried out.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Since bean paste has a water content of about 85%, a drying aid is added, dehydrated or dried to reduce the water content. This bean jam contains a large amount of sugars, proteins, crude fibers, and the like, and is used as a main raw material.

[0008] The bean jam is poor in sugars, amino acids, and vitamins necessary for germination of spores and initial development of hyphae when culturing filamentous fungi. Nutrition aids need to be supplemented. Rice bran, bran, okara, beer processing residue, shiitake processing residue, crab, etc. can be used as the organic nutritional supplement containing these components. These materials can be used alone or in appropriate mixtures, but all are equivalent to waste, can be obtained at low cost, and can be used economically. In the present invention, the filamentous fungus is a general term including ascomycetes, basidiomycetes, incomplete fungi, and algal fungi (excluding yeast).

[0009] The above bean jam is further added as an inorganic nutritional aid.
Inorganic salts such as potassium, magnesium, phosphorus, iron, zinc and the like are added alone or in a small amount as appropriate. In this case, it is more convenient to use plant ash or wood ash which contains the above-mentioned inorganic salts in a complex manner, since it may be sufficient to use these alone.

When culturing filamentous fungi, lowering the pH of the culture medium prevents invasion and growth of other germs and results in better growth of the filamentous fungi. Good. Acetic acid, wood acetic acid,
Malic acid, citric acid, lactic acid, corn steep liquor and other organic acids, and inorganic acids such as phosphoric acid may be used alone or in combination, and may be used by mixing with other auxiliaries or the following adjusted water. convenient.

During the cultivation, excessive stirring and mixing of the culture medium in order to improve the air permeability and maintain the temperature, moisture, and humidity often hinder growth and growth.
It is preferable to ensure an appropriate amount of moisture and air permeability in advance. For this purpose, peat moss, sponge, sawdust, bark, turtle, etc. are relatively breeding and are hard to decompose. In the case of a solid medium, it is convenient as a water conditioner. Such culture aids are commercially available as agricultural materials and can be obtained economically.

After the bean cake as the main raw material and the various auxiliaries are mixed and added, the mixture is mixed so that the whole is well dispersed,
It can be used directly as a medium in a state containing water. Further, the mixture can be used as a medium after the center temperature of the mixture is about 85 ° C. and the whole temperature is maintained for about 40 minutes. Furthermore, in order to reduce the water content and dry and facilitate transportation and storage, heat it under hot air at a maximum of 130 ° C. or less, and then stir appropriately under hot air at around 100 ° C.
%, And no residual filamentous fungi or other viable bacteria are found in such media. Its properties are slightly coarse powdery, and it is possible to adjust the particle size in some cases, and it can be further processed and processed into various forms such as pellets, plates and sheets.

[0013]

[Example] (Example 1) Dehydrated adzuki bean jam lees (water content 70%)
100kg, rice bran 10kg, wood ash 0.7kg, peat moss 5K
g, sawdust 5Kg, crab crab 5Kg, pH with lactic acid
After adjusting to 4.2, the medium was heated so that the center temperature could be maintained at 85 ° C. for 40 minutes to obtain a culture medium. When this medium was maintained for 7 days under a culture condition of 28 ° C. under aseptic conditions,
No occurrence of filamentous fungi was observed.

(Example 2) Red bean jam cake (water content: 85%) 8
0kg, 20kg of rice cake (85% water content), 1.6kg of rice bran
g, 0.6 kg of crab, 1 Kg of shiitake mushroom processing residue, and 0.6 g of charcoal powder.
After mixing with 0.7 kg, the mixture was adjusted to pH 4.2 with lactic acid, dried in a through-air drying oven at 135 ° C., and dried in 24 hours while lowering the aeration temperature to 90 ° C. in the latter half to prevent the occurrence of Maillard, etc. I let it. This allows the drying medium (water content 10
% Or less) 18 kg was obtained.

(Culture Example) Medium 4 obtained in Example 2 above
0 kg and 53 kg of warm water at 50 ° C. were mixed, and the antagonistic microorganism Trichoderma viride (trade name: Trichoderma AOK-31137, manufactured by Akita Konno Shoten Co., Ltd., Yoneya Co., Ltd.) (5 × 10 10 spores) ⁹ /
g) 0.65 kg was sprayed, mixed, and thoroughly mixed. The solid culture was left standing at a constant temperature of 24 ° C. and a humidity of 95% for 6 days, and then dried at 50 ° C. by ventilation. By this culture, a culture 3 containing 1 × 10 ¹⁰ / g of spores with a water content of 10% or less was obtained.
2 kg was obtained.

[0016]

According to the present invention, a large amount of filamentous fungi can be obtained by using bean jam which has been treated as waste as a main material and using an auxiliary which can be obtained relatively economically. A culture medium capable of performing pure solid culture of spores and the like is obtained. Since the medium thus obtained contains a large amount of solids, it is easy to perform processing such as separation and drying from the culture after culturing the filamentous fungus, and use the culture without separating the culture and the culture residue. It is economical because it can be done.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Ichiro Okuyama 500, Kamimachi, Narita-shi, Chiba Yoneya Co., Ltd. (72) Inventor Hiroshi Konno 248, Kariwano, Nishisenbokucho, Senboku-gun, Akita F, Akita Konno Shoten Co., Ltd. Terms (reference) 2B011 BA06 BA13 GA04 4B065 AA70X BB02 BB23 BB27 BC32 BC33 BC42 BD50 CA43 CA49 CA60

Claims (2)

[Claims] 1. A filamentous fungus whose water content is adjusted by using bean cake as a main raw material, and adding an organic nutritional aid such as rice bran, an inorganic nutritional aid such as potassium, a culture aid such as sponge, and a pH adjuster thereto. Culture medium. 2. A filamentous fungus whose main moisture is adjusted to low moisture by using bean jam as a main raw material, and adding an organic nutritional aid such as rice bran, an inorganic nutritional aid such as potassium, a culture aid such as sponge, and a pH adjuster. Culture medium.