CN103636406A - Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers - Google Patents
Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers Download PDFInfo
- Publication number
- CN103636406A CN103636406A CN201310660445.8A CN201310660445A CN103636406A CN 103636406 A CN103636406 A CN 103636406A CN 201310660445 A CN201310660445 A CN 201310660445A CN 103636406 A CN103636406 A CN 103636406A
- Authority
- CN
- China
- Prior art keywords
- sponge
- spawn
- paste
- edible fungus
- bacterial classification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers. The method comprises the following steps that firstly, corn flour and wheat flour are mixed, water is added to the mixed flour, paste is formed in a boiled mode, and the paste is cooled for standby application; secondly, the sponge is placed in the paste and stirred repeatedly, so that the paste permeates into holes of the sponge, then the sponge is pulled out, superfluous paste is squeezed, the sponge is placed in dry bran to be stirred and rolled over, and the bran adheres to the surfaces of the sponge; thirdly, the sponge with the bran in an adhering mode is placed in double-layer polypropylene bags uniformly, the surfaces are covered with fine wood chips, and sealing is conducted; fourthly, sterilization is conducted, inoculation is conducted after cooling, and cultivation is conducted. The method for manufacturing the edible fungus spawn is conducted by using the polyurethane sponge as the spawn carriers, the finished spawn is attached to the sponge after being cultivated, and the finished spawn can be processed into any physical shapes in an industrial and standardized mode; the finished spawn is attached to the sponge carriers, the finished spawn is easily separated from the sponge in a using process and is easily scattered in a pounded mode, and it can be avoided that the vitality of the spawn is damaged in an inoculation process because the spawn is bounded to pieces.
Description
Technical field
The present invention relates to the preparation method of bacterial classification for a kind of Edible Fungi, be specifically related to a kind of polyurethane sponge that utilizes as the preparation method of the edible fungus species of bacterial classification carrier.
Background technology
Edible fungus species is the seed of Edible Fungi, is the starting point of Edible Fungi.Select different materials to make vigor, quantity and use and keeping property that edible fungus species relates to bacterial classification.The present invention utilizes sponge and part grain class raw material to make edible fungus species not only to have simplified production of hybrid seeds operation, greatly improved the serviceability of bacterial classification, widened the production region of bacterial classification, increased work efficiency.
At present, edible fungus solid spawn comes minute to have two kinds by material, and a kind of is that timber, forage are the theme and add a small amount of grain, and a kind of is directly all to use grain (wheat berry, iblet or paddy), and manufacture craft is basically identical.Traditional edible fungus species all selects living beings as material, after simple process and processing, makes bacterial classification, and the physical aspect of bacterial classification is limited by the natural attribute of material substantially.
Traditional edible fungus species all selects living beings as material, after simple process or processing, makes bacterial classification, and the physical aspect of bacterial classification is limited by the natural attribute of material substantially, or needs very high processing cost just can meet instructions for use.And, finished product bacterial classification is in use not easy to operation, cannot accomplish that homogeneous is consistent in inoculum concentration, thereby is difficult to accomplish standardization, standardization, in seeded process, also can injure spawn activity because smashing bacterial classification to pieces, manufacturing process is time-consuming simultaneously, cost is higher.
Summary of the invention
The object of this invention is to provide a kind of polyurethane sponge that utilizes as the preparation method of the edible fungus species of bacterial classification carrier, the method is usingd polyurethane sponge as carrier, and finished product bacterial classification uniformly appendix, on sponge, does not need in use procedure to smash to pieces.
In order to reach above-mentioned technique effect, the present invention takes following technical scheme:
Utilize polyurethane sponge as a preparation method for the edible fungus species of bacterial classification carrier, comprise the following steps:
Step 1: corn flour and wheat flour are mixed, add water and be brewed into paste, cooling standby;
Step 2: sponge is put into paste and repeatedly stir, paste is infiltrated in sponge hole, then pull the unnecessary paste of extruding out, then put into dry wheat bran and stir rolling, make sponge surface adhesion wheat bran;
Step 3: evenly pack the sponge that adheres to wheat bran into double-deck Polypropylene Bag, the thin wood chip of surface coverage, sealing;
Step 4: sterilizing, cooling rear inoculation, cultivates.
Further technical scheme is: described corn flour and the mass ratio of wheat flour are: (2～5): 1; The particle diameter of corn flour is below 40 orders.
Further technical scheme is: the mass fraction of described paste is 20～30%.
Further technical scheme is: described sponge is that perforate is the polyurethane porous sponge of 1～3mm.
Further technical scheme is: being shaped as of described sponge is spherical, disk, square or square bar, can be according to user demand, and sponge can also be arbitrary shape.
Further technical scheme is: described sterilising temp is 123 ℃, and sterilization time is 180min.
The present invention compared with prior art, has following beneficial effect:
(1) preparation method of this bacterial classification adopts sponge as carrier, after finished product breeding strain, appendix is on sponge, can industrialized standard finished product bacterial classification be processed into any physical shape, finished product bacterial classification appendix is on sponge carrier, in use finished product bacterial classification is very easily separated with sponge, very easily smash loosely, in seeded process, can not injure spawn activity because smashing to pieces.
(2) adopt sponge as carrier, corn flour, wheat flour and wheat bran be as nutrient source, the bacterial classification water binding capacity of cultivation, good air permeability, and mycelial growth is healthy and strong rapidly.
(3) the uniform appendix of finished product bacterial classification that the present invention cultivates, on sponge carrier, can reach inoculum concentration homogeneous consistent during inoculation, accomplish standardization and standardization.
(4) preparation method of this bacterial classification is applicable to the breeding strain of different mushrooms; Meanwhile, the material source in this method extensively, is not subject to the restrictions such as season, region and material.
Embodiment
Below in conjunction with embodiments of the invention, the invention will be further elaborated.
Embodiment 1:
The preparation method of pleurotus eryngii quel strains:
Step 1: material and formula
Corn flour 800g, wheat flour 200g, porous sponge blockage 20mm * 20mm * 150mm500g, dry wheat bran is appropriate, wooden branch 170mm * 7mm * 7mm(soaks 23 hours with 3% lime poach in advance for 1 hour again).Xingbao mushroom is produced generally and need to be punched at bacterium bag SMIS, thereby while inoculating, bacterial classification is accessed to bacterium bag bottom, and to guarantee that mycelia grows from upper, middle and lower simultaneously, the object of using branch is to be convenient to inoculation operation.
Step 2: paste is made
The corn flour preparing, wheat flour are joined in 3 liters of cold running waters and stirred, be heated to boiling and boil 20 minutes, be brewed into paste, cooling standby.
Step 3: wrap up in material
Sponge block is mixed in paste and repeatedly stirred, make paste infiltrate sponge hole completely, then pull extruding out, unnecessary paste extruding is removed.Be poured in dry wheat bran and stir rolling, make sponge block surface adhesion wheat bran; Wood branch is pulled out and is used clear water shower one time after adopting limewash to soak, and in excessive dry wheat bran, repeatedly turns and makes it fully to adhere to wheat bran.
Step 4: dress bag
At 17cm * 35cm * 5s(5s=0.05mm) Polypropylene Bag in a side first pack 37 branches into, evenly pack the sponge block that wraps material into 37 again, the thin wood chip of surface coverage one deck 2cm thickness, SMIS inserts one of long 180mm diameter 22mm sticking plaster, the 3.3cm bore loudspeaker collar and supporting nonwoven lid seal.It is in order when inoculating, original seed piece to be accessed to bacterial classification bag SMIS bottom, to guarantee that mycelia grows from upper, middle and lower simultaneously that SMIS inserts sticking plaster, shortens the breeding strain cycle.
Step 5: sterilizing
The rear dress basket of having packed pushes pressure cooker sterilizing, 123 ℃ of sterilising temps, sterilization time 180min.
Step 6: cooling inoculation and cultivation
After sterilizing finishes, be cooled to 28 ℃ of bag core temperature and pull out the sticking plaster access original seed in bag when following, then proceed to dark cultivation the in 23～25 ℃, breeding strain storehouse, until mycelia is covered with bacterium bag.
Step 7: finished product
After mycelia is covered with, reject bacteria infection and have damaged strain bag, select that mycelial growth is vigorous, pure white stalwartness be finished product bacterial classification.
Bacterial classification cultivation cycle 26 days, the dense stalwartness of mycelia, during use, sponge bacterial classification piece is very easily smash loosely, inserts a sponge bacterial classification piece of a branch rear surface access during inoculation Xingbao mushroom bacterium bag, without effort, smashes loose capping kind.24 hours actication of culture time, fast 12 hours of the bacterial classification of making than conventional method, inoculum concentration stable homogeneous, handled easily, effect fast 1/3rd.
Embodiment 2:
The preparation method of agrocybe bacterial classification:
Step 1: material and formula
Corn flour 800g, wheat flour 200g, porous sponge blockage 40mm * 40mm * 150mm300g, dry wheat bran is appropriate.
Step 2: paste is made
The corn flour preparing, wheat flour are joined in 3 liters of cold running waters and stirred, be heated to boiling and boil 20 minutes, be brewed into paste, cooling standby.
Step 3: wrap up in material
Sponge block is mixed in paste and repeatedly stirred, make paste infiltrate sponge hole completely, then pull extruding out, unnecessary paste extruding is removed.Be poured in dry wheat bran and stir rolling, make sponge block surface adhesion wheat bran.
Step 4: dress bag
At 15cm * 33cm * 5s(5s=0.05mm) Polypropylene Bag in the sponge block that wraps material is evenly loaded to about work loading height 180mm, the thin wood chip of surface coverage one deck 2cm thickness, the 3.3cm bore loudspeaker collar and supporting nonwoven lid seal.
Step 5: sterilizing
The rear dress basket of having packed pushes pressure cooker sterilizing, and sterilising temp is 123 ℃, and sterilization time is 180min.
Step 6: cooling inoculation and cultivation
After sterilizing finishes, be cooled to 28 ℃ of bag core temperature and access original seed when following, then proceed to dark cultivation the in 23～25 ℃, breeding strain storehouse, until mycelia is covered with bacterium bag.
Step 7: finished product
After mycelia is covered with, reject bacteria infection and have damaged strain bag, select that mycelial growth is vigorous, pure white stalwartness be finished product bacterial classification.
Bacterial classification cultivation cycle 20 days, the dense stalwartness of mycelia, during use, sponge bacterial classification piece is very easily smash loosely, and during inoculation agrocybe bacterium bag, a sponge bacterial classification piece of surface access, smashes loose capping kind without effort.24 hours actication of culture time, fast 24 hours of the bacterial classification of making than conventional method, inoculum concentration stable homogeneous, handled easily, effect is fast again.
Although with reference to explanatory embodiment of the present invention, invention has been described here, above-described embodiment is only preferably embodiment of the present invention, embodiments of the present invention are not restricted to the described embodiments, should be appreciated that, those skilled in the art can design a lot of other modification and embodiments, and these are revised and within embodiment will drop on the disclosed principle scope and spirit of the application.
Claims (6)
1. utilize polyurethane sponge as a preparation method for the edible fungus species of bacterial classification carrier, it is characterized in that comprising the following steps:
Step 1: corn flour and wheat flour are mixed, add water and be brewed into paste, cooling standby;
Step 2: sponge is put into paste and repeatedly stir, paste is infiltrated in sponge hole, then pull the unnecessary paste of extruding out, then put into dry wheat bran and stir rolling, make sponge surface adhesion wheat bran;
Step 3: evenly pack the sponge that adheres to wheat bran into double-deck Polypropylene Bag, the thin wood chip of surface coverage, sealing;
Step 4: sterilizing, cooling rear inoculation, cultivates.
2. the polyurethane sponge that utilizes according to claim 1, as the preparation method of the edible fungus species of bacterial classification carrier, is characterized in that described corn flour and the mass ratio of wheat flour are: (2～5): 1; The particle diameter of corn flour is below 40 orders.
3. the polyurethane sponge that utilizes according to claim 1, as the preparation method of the edible fungus species of bacterial classification carrier, is characterized in that the mass fraction of described paste is 20～30%.
4. the polyurethane sponge that utilizes according to claim 1, as the preparation method of the edible fungus species of bacterial classification carrier, is characterized in that described sponge is that perforate is the polyurethane porous sponge of 1～3mm.
5. the polyurethane sponge that utilizes according to claim 1 is as the preparation method of the edible fungus species of bacterial classification carrier, it is characterized in that being shaped as of described sponge is spherical, disk, square or square bar.
6. the polyurethane sponge that utilizes according to claim 1, as the preparation method of the edible fungus species of bacterial classification carrier, is characterized in that described sterilising temp is 123 ℃, and sterilization time is 180min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310660445.8A CN103636406B (en) | 2013-12-05 | 2013-12-05 | Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310660445.8A CN103636406B (en) | 2013-12-05 | 2013-12-05 | Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103636406A true CN103636406A (en) | 2014-03-19 |
| CN103636406B CN103636406B (en) | 2015-03-11 |
Family
ID=50241825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310660445.8A Active CN103636406B (en) | 2013-12-05 | 2013-12-05 | Method for manufacturing edible fungus spawn by using polyurethane sponge as spawn carriers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103636406B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5634243B2 (en) * | 1978-11-29 | 1981-08-08 | ||
| JPS63109724A (en) * | 1986-10-29 | 1988-05-14 | 宮脇 彬行 | Culture of shiitake |
| JPH01181729A (en) * | 1988-01-13 | 1989-07-19 | Kanebo Ltd | Medium for mushroom seed fungus |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| JPH0416123A (en) * | 1990-05-09 | 1992-01-21 | House Food Ind Co Ltd | Medium for mushroom |
| KR970068810A (en) * | 1997-08-05 | 1997-11-07 | 이재일 | Seed culture and cultivation method of pupa cordyceps sinensis using silkworm pupa |
| JP2001128547A (en) * | 1999-11-02 | 2001-05-15 | Yasutoshi Sato | Mushroom culture with kenaf, a plant originating from africa |
| CN2452269Y (en) * | 1999-08-27 | 2001-10-10 | 邹书海 | Cultivation material bag for edible mushrooms |
| JP2002204685A (en) * | 2001-01-09 | 2002-07-23 | Yoneya Kk | Culture medium for cultivating filamentous fungus |
| KR20060010454A (en) * | 2004-07-28 | 2006-02-02 | 한상노 | Method of human work cultivation by using high density of phellinus linteus |
-
2013
- 2013-12-05 CN CN201310660445.8A patent/CN103636406B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5634243B2 (en) * | 1978-11-29 | 1981-08-08 | ||
| JPS63109724A (en) * | 1986-10-29 | 1988-05-14 | 宮脇 彬行 | Culture of shiitake |
| JPH01181729A (en) * | 1988-01-13 | 1989-07-19 | Kanebo Ltd | Medium for mushroom seed fungus |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| JPH0416123A (en) * | 1990-05-09 | 1992-01-21 | House Food Ind Co Ltd | Medium for mushroom |
| KR970068810A (en) * | 1997-08-05 | 1997-11-07 | 이재일 | Seed culture and cultivation method of pupa cordyceps sinensis using silkworm pupa |
| CN2452269Y (en) * | 1999-08-27 | 2001-10-10 | 邹书海 | Cultivation material bag for edible mushrooms |
| JP2001128547A (en) * | 1999-11-02 | 2001-05-15 | Yasutoshi Sato | Mushroom culture with kenaf, a plant originating from africa |
| JP2002204685A (en) * | 2001-01-09 | 2002-07-23 | Yoneya Kk | Culture medium for cultivating filamentous fungus |
| KR20060010454A (en) * | 2004-07-28 | 2006-02-02 | 한상노 | Method of human work cultivation by using high density of phellinus linteus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103636406B (en) | 2015-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105272525B (en) | The edible fungus industrial cultivation matrix and its application of coco bran preparation | |
| CN104303846B (en) | Method with the big bag cultivating Pleurotus geesteranus of fermentation material | |
| JP6548124B2 (en) | A culture solution for mulberry cultivation, a culture medium for mulberry cultivation, a method for producing a culture liquid for mulberry cultivation, and a cultivation method for mulberry | |
| CN103650903A (en) | Industrialized production method for flammulina velutipes in bags | |
| KR101464165B1 (en) | Culture medium composition for mushroom's species cultivation using food waste compost | |
| CN101731097A (en) | Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain | |
| CN102657028A (en) | Soil covering culture method for flower mushrooms | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN104798602B (en) | Pleurotus eryngii industrial production method | |
| WO2014010314A1 (en) | Method for cultivating mushrooms using reusable fiber substrate, and culture medium for cultivation using same | |
| CN107736184A (en) | A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn | |
| CN105123260A (en) | Method for increasing summer mushroom output | |
| CN104488546A (en) | Pleurotus geesteranus planting method | |
| CN101803533A (en) | Method for cultivating edible fungi | |
| CN103371053A (en) | Method for cultivating green natural oyster mushrooms by using pure-length rice straws | |
| CN102487727A (en) | Method for producing pleurotus eryngii quel strains | |
| JP2011078394A (en) | Fermentation medium for edible mushroom and production method therefor | |
| CN104472219A (en) | Agrocybe cylindracea cultivation method | |
| CN104285677B (en) | A kind of preparation method of edible mushroom peg wood bacterial classification | |
| Pardo-Gimenez et al. | Supplementation of high nitrogen Agaricus compost: yield and mushroom quality | |
| JP2006262742A (en) | Mushroom-bed cultivation method, and small apparatus for producing mushroom bed | |
| KR20140127390A (en) | Culture medium composition for Flammulina velutipes and its preparation method, and a method of cultivation mushroom using it | |
| CN106718014B (en) | Earthing soil for agaricus bisporus cultivation and preparation method thereof | |
| CN103396218A (en) | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost | |
| CN103396231A (en) | Culture material composition for straw mushroom breeder seeds, and preparation method of culture material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 611700 five groups of Zhan village, Tang Chang town, PI Du District, Chengdu, Sichuan Patentee after: Chengdu Yan Rongzhen industry limited company Address before: 610000 group 5, Zhan Qi Village, Tang Chang town, Pixian, Chengdu, Sichuan Patentee before: Chengdu Rongzhen Mushroom Industry Co.,Ltd. |