JPH0416123A - Medium for mushroom - Google Patents

Abstract

PURPOSE:To promote the growth of mushroom mycelium by adding a baked porous material prepared by using wheat flour as main raw material. CONSTITUTION:A baked material having porous texture and prepared by using wheat flour as main raw material (e.g. bread) is mixed with sawdust having particle size of 10-32 mesh at a volume ration of 1/(2-6) to obtain a medium for mushroom. Water is added to the medium to a water-content of 62-68wt.% and the mixture is filled in a vessel to a density of 0.6-0.7g/cm<3> and sterilized by heating at 100-121 deg.C for 15-60 min. Wood-destroying fungi are implanted to the medium and cultured at 20-27 deg.C in dark place.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a culture medium for fungi, and more specifically, uses a baked product having a porous structure made of wheat flour as the main raw material as a nitrogen source. This invention relates to an artificial culture medium for occlusans.

2. Traditionally, mushroom cultivation has traditionally been based on log cultivation, but in recent years, artificial medium cultivation using sawdust has become popular. Mushrooms that can be cultivated include nameko, oyster mushroom, enokitake, maitake,
The number of types of mushrooms, such as shiitake and kuritake, is gradually increasing. On the other hand, the frequency of abnormal weather events is increasing year by year, and the destruction of nature due to urban development is progressing, making it impossible to expect a stable supply of mushrooms by cultivating logs, making it necessary to cultivate mushrooms indoors in an artificial environment. It is thought that gender will become more and more important. Currently, the media used for artificial culture cultivation are mainly sawdust and rice bran, with sawdust being used as a carbon source and rice bran as a nitrogen source. The nutrients contained in rice bran include protein, inorganic salts, and vitamins, and rice bran is currently said to be the best nitrogen source. However, the ingredients in rice bran tend to change easily and are unstable, so care must be taken in storage management. Additionally, bran is often used as a nitrogen source along with rice bran, and the same can be said for this as well.

[Problems to be Solved by the Invention] Therefore, the present invention provides a culture medium for open-capped species that is relatively stable and has a sufficient supply, and contains a nitrogen source that further promotes the growth of mushroom mycelium, in place of rice bran and bran. The purpose is to provide

: Means for Solving the Problem] The present invention was made based on the knowledge that when white bread is used as a nitrogen source for an artificial culture medium for mushrooms, mushroom mycelium grows faster than when using rice bran or bran. be.

That is, the present invention provides a culture medium for open lids, which is characterized by containing a baked product having a porous structure and using wheat flour as a main raw material.

The medium composition in the present invention is similar to that found in general sawdust cultivation of mushrooms, except for the nitrogen source. That is, it includes a baked product with a porous structure mainly made of wheat flour as a nitrogen source, sawdust such as beech or cedar as a carbon source, and water. If desired, a common nitrogen source such as rice bran or bran can be co-existed in an amount of 2/3 or less of the weight of the baked product, but the smaller the amount, the better. It is better not to let it happen.

Examples of baked goods having a porous structure made of wheat flour as a main ingredient include breads such as white bread and French bread, and castella cakes. (Hereinafter, the baked product with a porous structure made of wheat flour as the main ingredient will be referred to as the baked product of the present invention.) The baked product of the present invention can be made into powder or cut into cubes depending on the characteristics of the type of mushroom. and can be used.

Commercially available sawdust can be used as is, but it is best to use sawdust with a size of 10 mesh to 32 mesh.

In the present invention, the blending ratio of the fired product of the present invention and sawdust is not particularly limited, but the volume ratio is 1/2.
It is preferable to mix the ingredients so that the ratio is 1/6 to 1/6, preferably 1/3 to 1/4. Next, water is preferably added to this mixture to adjust the water content to, for example, 62 to 68% by weight.

The prepared medium PP is then placed in commonly used containers such as (polypropylene) bottles at a density of e.g.
．． Fill to 6 to 0.7 g/cnf.

Next, heat it in an autoclave at 100-121℃ for 15 minutes.
Heat sterilize for ~60 minutes. Next, bacteria previously cultured in a similar medium are inoculated into the above medium, and cultured at 20 to 27°C in the dark.

As wood-decaying fungi to be cultured in the medium of the present invention, Lentinus genus such as shiitake, Grifol such as maitake, etc.
Examples include mushrooms of the genus A and Fistulina such as daylily.

[Effects of the Invention] According to the present invention, it is possible to provide a medium in which when rice bran or bran is used as a nitrogen source, the mycelium of mushrooms grows faster.

Next, the present invention will be explained with reference to examples.

[Examples] Example 1 (Medium composition) Carbon source Beech sawdust Nitrogen source A, 8.5 mm square cut bread of 11 mesh bass bread C911 mesh bass bread - rice bran D, 5 mm square cut bread - rice bran E, rice bran (control ) *Bread: Rice bran 1:1 (weight ratio)

*5mm x 5mm cubes... 5 m long bread with ears removed
Cut into m square pieces.

Culture medium materials containing the above five combinations of carbon sources and nitrogen sources were prepared. These five types of combined culture medium materials were uniformly mixed at a carbon source: nitrogen source = 3:1 (volume ratio), and then placed in a plant culture test tube (inner diameter 22 mm,
Fill it to a height of 150mm) and add water to it to make the moisture content 68.
The weight percent and density were adjusted to 0.7 g/ctl.

The height of the medium at this time was 9 to 10 cm. next,
This test tube was heat sterilized at 121° C. for 15 minutes using an autoclave. After cooling, small pieces of shiitake mushroom mycelia cultured on agar were inoculated onto the surface of the medium. Next, this was placed in a 25°C incubator and cultured in the dark.

Table 1 shows the degree of mycelial growth (infestation) of shiitake mushrooms at this time. In addition, the growth rate is determined by the height of the medium as a, and the inoculation surface (surface).
When the distance from to the tip of the hyphae is b, (b/a)
Calculated from x 100.

As is clear from Table 1, the degree of mycelial growth is greater in the cases where bread is used as the nitrogen source (A, B, C, D) than in the case where only rice bran is used (E). It can be seen that the mycelial growth rate of the present invention (A, B, C, D) is faster than that of the comparative example (E). In addition, the degree of mycelium growth was greater in the products using only white bread (A and B) than in the products using white bread mixed with rice bran (C and D).
Therefore, it can be seen that the growth rate of mycelium is faster in those using only white bread (A and B) than in those using white bread mixed with rice bran (C and D).

Claims (2)

[Claims] (1) A culture medium for fungi, which is characterized by containing a baked product having a porous structure made of wheat flour as a main ingredient. (2) The medium for fungi according to claim 1, which is used as a medium for wood-decaying fungi.