CN103621481A - Indoor evaluating method for repellant effects of pest repellents - Google Patents
Indoor evaluating method for repellant effects of pest repellents Download PDFInfo
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- CN103621481A CN103621481A CN201310655046.2A CN201310655046A CN103621481A CN 103621481 A CN103621481 A CN 103621481A CN 201310655046 A CN201310655046 A CN 201310655046A CN 103621481 A CN103621481 A CN 103621481A
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Abstract
The invention discloses an indoor evaluating method for repellant effects of pest repellents. According to the method, host plant branches which are treated by the pest repellants and are used as the control are respectively placed in branch cups arranged at the two sides of an experiment pest cage, then, sufficient experiment pests are fed from a pest feeding opening, the experiment pest cage is placed into an artificial climate box suitable for experiment pests to live, and the repellant effects of the pest repellents are observed after the preset time. A middle separation plate is arranged in the middle of the experiment pest cage for diving the experiment pest cage to form a left side pest cage and a right side pest cage, a pest feeding groove is formed in the top of the middle separation plate and is respectively communicated with the left side pest cage and the right side pest cage through a test pest outlet, the pest feeding opening capable of being opened and closed is formed in the cage wall above the pest feeding groove, screen ventilation openings are respectively formed in the side walls of the left and right side pest cages, screen covers are respectively arranged on the tops of the left and right side pest cages, and the branch cups capable of being used for placing host plant branches are also arranged in the left and right pest cages.
Description
Technical field:
The present invention relates to a kind of indoor evaluation method of insect repellent, be specifically related to the indoor evaluation method of the insects repellant repellent effects such as a kind of oranges and tangerines wood louse (Diaphorina citri Kuwayama).
Background technology:
Common insects repellant can be used for skin, makes skin exempt from biting of mosquito, mite, lice, worm; Also can be used for packing, the product that prevents from storing in a warehouse suffers the destruction of insect.Current, insects repellant application aspect control sanitary insect pest is comparatively general, as culicifuge and cockroach repellant etc.And, for the repellant of indoor sanitary insect pest, there is relevant evaluation method (if GBT13917.9-2009 agriculture chemical registration is with hygienic insecticide indoor pharmacodynamic test and evaluate the 9th part: repellant).
Compare with sanitary insect pest repellant with insecticide, the repellant development of control agriculture and forestry injurious insect is comparatively slow.But along with the raising of people's environmental consciousness and the reinforcement to self health concerns consciousness, the cry of green agriculture is more and more higher, and insects repellant will be played the part of more key player in control pest.In the process that the mankind and pest are struggled, the idea of agricultural insect pests control is also progressively being changed.By the laying eggs or take food of pest, reach the object of Control pests, this method more and more comes into one's own.
Therefore the repellant of preventing and treating pest will have wide market prospects.Repellant is as a kind of special application field of insecticide, and its application potential has to be excavated.The research of this respect just more and more comes into one's own.In recent decades, Chinese scholars has been done a large amount of research work to the repellent activity material of agriculture and forestry injurious insect, also from a large amount of plants extracting many activated compositions.By preventing and treating function, can be divided into two class materials, insect is had and takes food repellant can being divided into of repellent action and the large class of repellant two of laying eggs.
It should be noted that for agriculture and forestry injurious insect and there is no good System Assessment Method.
Summary of the invention:
The indoor evaluation method that the object of this invention is to provide a kind of insects repellant repellent effect.
The indoor evaluation method of insects repellant repellent effect of the present invention, it is characterized in that, in the branch cup of both sides of testing worm cage, place respectively through insects repellant host plant branch that process and in contrast, then access enough experiment insects connecing insect population, then experiment worm cage being positioned over to experiment insect is applicable to, in the climatic cabinate of existence, observing the repellent effect of insects repellant through predetermined time;
Described experiment worm cage, being provided with therebetween central dividing plate will test worm cage and separately form left and right side worm cage, at the top of central dividing plate, be provided with and connect worm groove, connecing worm groove tries worm outlet by confession and communicates with left and right side worm cage respectively, on the cage wall connecing above worm groove, be provided with can folding the insect population that connects, on the sidewall of left and right side worm cage, be all provided with grenadine ventilating opening, the top of left and right side worm cage is all provided with band grenadine lid, is also provided with the branch cup that can place host plant branch in left and right side worm cage.
The described angle between the outlet of examination worm that leads to left and right side worm cage is preferably acute angle.
Described is experiment worm taxis behavior outlet for the outlet of examination worm, and wide 1～2mm, is highly less than 5mm, as 4mm etc.
Described experiment worm cage is preferably the experiment worm cage that transparent material is made, and as the plastic material with transparent or glass, makes, and is easy to test the observation of insect.
Compared with prior art, beneficial effect of the present invention is as follows:
1) compare with existing Y-piece, the present invention has shortened the distance of creeping of experiment insect, contributes to like this observation of experimental result, the insect of moving by jump especially.
2) from centre, be spaced apart both sides, effectively stop and process the interference of branch to contrast branch, improved the accuracy of repellant laboratory experiment.
Accompanying drawing explanation:
Fig. 1 is the structural representation of experiment worm cage of the present invention;
Fig. 2 is the vertical view of the experiment worm cage in Fig. 1;
1, connect insect population; 2, for the outlet of examination worm; 3, connect worm groove; 4, central dividing plate; 5, grenadine ventilating opening; 6, host plant branch; 7, right side worm cage; 8, branch cup; 9, band grenadine lid; 10, left side worm cage.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
As shown in Figure 1, the experiment worm cage of the present embodiment, it is by transparent plastic production, be provided with therebetween central dividing plate 4 and will test a separately formation left side of worm cage, right both sides worm cage, worm cage 10 and right side worm cage 7 on the left of, at the top of central dividing plate, be provided with and connect worm groove 3, connecing worm groove 3 tries worm outlet 2 by confession and communicates with left side worm cage 10 and right side worm cage 7 respectively, the described angle between the outlet of examination worm that leads to left side worm cage 10 and right side worm cage 7 is acute angle, on the cage wall connecing above worm groove 3, be provided with can folding connect insect population 1, on the sidewall of left side worm cage 10 and right side worm cage 7, be all provided with respectively grenadine ventilating opening 5, on the sidewall of left side worm cage 10 and right side worm cage 7, be also all provided with respectively band grenadine lid 9, on the sidewall of left side worm cage 10 and right side worm cage 7, be all provided with respectively the branch cup 8 that can place host plant branch 6.
To in the branch cup 8 of the both sides of experiment worm cage, place respectively through oranges and tangerines wood louse repellants (active ingredient is guava extract and paraffine base crude oil) and process and contrast the host plant kamuning branch 6 that clear water is processed, then connecing insect population access 60 oranges and tangerines wood louses (female-male proportion is 1:1).For examination oranges and tangerines wood louse connect worm groove 3 will be from climbing out of to both sides kamuning branch for examination worm outlet 2.Experiment worm cage is positioned in the climatic cabinate that is applicable to the existence of oranges and tangerines wood louse.The time of setting through overtesting, observe the stop situation of oranges and tangerines wood louse on the kamuning branch of both sides.
Concrete outcome is as follows:
Table 1: the Lab-evaluation of oranges and tangerines wood louse repellant effect
| One side worm cage | Opposite side worm cage |
| Kamuning branch is through repellant ①Process | Clear water control treatment |
| 80±9% | 14±9% |
| Clear water control treatment | Kamuning branch is through repellant ②Process |
| 9±6% | 88±6% |
| Blank (kamuning branch is without chemicals treatment) | Blank (kamuning branch is without chemicals treatment) |
| 46±9% | 47±10% |
1. 1L repellant is containing 20g guava extract+10ml95%(volume fraction) paraffine base crude oil (aqueous solution);
2. 1L repellant is containing 30g guava extract+20ml95%(volume fraction) paraffine base crude oil (aqueous solution).
As can be seen from Table 1, of the present invention can be well in the effect of walking quickly and keeping away of the repellant of Lab-evaluation oranges and tangerines wood louse.
Claims (4)
1. the indoor evaluation method of an insects repellant repellent effect, it is characterized in that, in the branch cup of both sides of testing worm cage, place respectively through insects repellant host plant branch that process and in contrast, then access enough experiment insects connecing insect population, experiment worm cage is positioned over to experiment insect to be applicable to, in the climatic cabinate of existence, observing the repellent effect of insects repellant through predetermined time;
Described experiment worm cage, being provided with therebetween central dividing plate will test worm cage and separately form left and right side worm cage, at the top of central dividing plate, be provided with and connect worm groove, connecing worm groove tries worm outlet by confession and communicates with left and right side worm cage respectively, on the cage wall connecing above worm groove, be provided with can folding the insect population that connects, on the sidewall of left and right side worm cage, be all provided with grenadine ventilating opening, the top of left and right side worm cage is all provided with band grenadine lid, is also provided with the branch cup that can place host plant branch in left and right side worm cage.
2. indoor evaluation method according to claim 1, is characterized in that, the described angle between the outlet of examination worm that leads to left and right side worm cage is acute angle.
3. indoor evaluation method according to claim 1, is characterized in that, described is experiment worm taxis behavior outlet for the outlet of examination worm, and wide 1～2mm, is highly less than 5mm.
4. indoor evaluation method according to claim 1, is characterized in that, described experiment worm cage is the experiment worm cage that transparent material is made.
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| CN103621481B CN103621481B (en) | 2015-07-01 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107515294A (en) * | 2017-08-14 | 2017-12-26 | 无限极（中国）有限公司 | The method of testing of repellent worm effect |
| CN108072735A (en) * | 2016-11-10 | 2018-05-25 | 成都彩虹电器（集团）股份有限公司 | A kind of spatial repellency product indoor pharmacodynamic test method |
| TWI710318B (en) * | 2020-01-15 | 2020-11-21 | 國立高雄大學 | Drug effect test device for mosquito repellent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108072735A (en) * | 2016-11-10 | 2018-05-25 | 成都彩虹电器（集团）股份有限公司 | A kind of spatial repellency product indoor pharmacodynamic test method |
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| TWI710318B (en) * | 2020-01-15 | 2020-11-21 | 國立高雄大學 | Drug effect test device for mosquito repellent |
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Address after: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Patentee after: Guangdong Institute of biological resources application Address before: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Patentee before: Guangdong Entomological Institute |
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Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Guangdong Institute of Applied Biological Resources |