CN103599447B - For preventing the Chinese medicine immune formulation of infections chicken cloacal bursa - Google Patents
For preventing the Chinese medicine immune formulation of infections chicken cloacal bursa Download PDFInfo
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Abstract
The invention provides a kind ofly for preventing the Chinese medicine immune formulation of infections chicken cloacal bursa, its bulk drug comprises the Radix Astragali, the Chinese bulbul, hairyvein agrimony, eclipta, barrenwort, aleppo avens, the coptis, Samson St Johswort, adenophora tetraphylla, Chinese gall, Kaempferia galanga, wilsonii, garlic, nutmeg, radish seed, Java brucea, the wind-weed, the fruit of glossy privet, hawthorn, Rose Mallow Root, sealwort, sensitive plant, Radix Codonopsis, kamuning, the fruit of Chinese wolfberry, perilla seed and cordate houttuynia. This Chinese medicine immune formulation has significant effect in prevention aspect infections chicken cloacal bursa, and have nontoxic, the advantage of resistance, drug residue free.
Description
Technical field
The present invention relates to traditional Chinese medicine technical field, relate in particular to a kind of for preventing infections chicken cloacal bursaChinese medicine immune formulation.
Background technology
Bursal Disease (IBDV) claim again infectious bursal disease, is by infectious bursa of FabriciusOne that virus causes is acute, contact, inhibitive ability of immunity disease, with bursa of farbricius inflammation, necrosis, witherContracting and bursa of farbricius endolymph cell are badly damaged as feature, and clinical manifestation is lassitude, apocleisis, indirectProperty is suffered from diarrhoea, is trembled and severe weakness. There is the very strong pathogenic and higher death rate. Under normal circumstancesPathogenicity rate is 40～60%, and the death rate is generally 8～25%.
The lymphoid tissues such as the humoral immunity maincenter organ bursa of farbricius of the main infringement of this disease chicken, due to immunosupressCause secondary and concurrent other diseases, cause the death rate obviously to raise, bring major disaster to poultry husbandry,Being called as " AIDS of chicken ", is one of topmost disease of current aviculture.
This disease incubation period is 2～3d, has some cases to show as without any symptom and search for food suddenly when morbidityAmount increase, second day sharply decline, in chicken group, there is suddenly the chicken of off color, and spread very soon toQuan Qun; Sick chicken down in spirits, chilly weakness, feather are fluffy and disorderly, drop-head closes order, two wings are sagging, squatOn the ground, walk unstable, feed intake reduces, drinking-water increases, some is from pecking anus, row's thin rice gruel sample or egg whiteSample ight soil, severe one dehydration, sleeping ground do not rise, extremely feeble, finally dead. Death mostly occurs after being ill3～4d left and right, presents typical " death of spike formula ". The resistance to Anemia in Chicken of crossing is become thin, poor growth.
Chicken infectivity bursa of Fabricius virus is mainly encroached on the immune organ of chicken, causes immunosupress, makes disease chickenMore other virulence factors of easy infection, thereby the key for the treatment of is to improve the immunologic function of chicken.
The infections chicken cloacal bursa vaccine that control infections chicken cloacal bursa uses is mainly domestic and importedThe weak malicious seedling of infections chicken cloacal bursa or oil seepage, used immune effect undesirable for a long time, and many areas are being usedThe immunity of infections chicken cloacal bursa commercially available vaccine often has infections chicken cloacal bursa outbreak of epidemic afterwards, and the incidence of disease andThe death rate is growing on and on, for this reason, seek a kind of toxicity low, have no drug resistance, noresidue in livestock productsNew substitute or adminicle are imperative.
Traditional veterinary science extensive knowledge and profound scholarship of motherland, cure the disease focus on entirety and treat both principal and secondary aspect of disease, with Chinese herb on the prevention shouldDisease has unique curative effect. This disease is subordinate to the category of Chinese veterinarian's " pestilence ". Its etiology and pathogenesis is the invasion and attack of heat poison,And present heat in both exterior and interior, or hot poison spreads the three cloudy danger that internal organs cause and waits, and this disease is directly to be entered by warm heresyGas divides to be compeled to turn blood system and causes that qi and blood two broadcasts, and intense heat in the blood, due to the hot malicious progress of disease. Natural Chinese medicinal herb control shouldSick general principle is strengthening vital QI to eliminate pathogenic factors, ensures healthy physiological function and eliminate paathogenic factor to strengthen anti-Sick ability, curative mechanism can be summarized as direct antivirus action and the immunologic function of chicken is regulated to two aspects.The present invention is exactly again upper propose a kind of for preventing the Chinese medicine immunity system of infections chicken cloacal bursa in this basisAgent, it has good result for the treatment of.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of for preventing the Chinese medicine of infections chicken cloacal bursaImmune formulation, it has significant effect aspect prevention infections chicken cloacal bursa, and has nontoxic, resistance toThe advantage of medicine, drug residue free.
For solving the problems of the technologies described above, the invention provides a kind of for preventing infections chicken cloacal bursaMedicine immune formulation, its bulk drug comprises the Radix Astragali, the Chinese bulbul, hairyvein agrimony, eclipta, barrenwort, blue clothJust, the coptis, Samson St Johswort, adenophora tetraphylla, Chinese gall, Kaempferia galanga, wilsonii, garlic, nutmeg, radishSon, Java brucea, the wind-weed, the fruit of glossy privet, hawthorn, Rose Mallow Root, sealwort, sensitive plant, Radix Codonopsis, kamuning,The fruit of Chinese wolfberry, perilla seed and cordate houttuynia.
Wherein, in described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively:
The Radix Astragali 75 weight portion～85 weight portions, the Chinese bulbul 35 weight portion～45 weight portions, hairyvein agrimony 25Weight portion～35 weight portion, eclipta 40 weight portion～50 weight portions, barrenwort 25 weight portion～35Weight portion, aleppo avens 45 weight portion～55 weight portions, the coptis 55 weight portion～65 weight portions, Samson St Johswort30 weight portion～40 weight portions, adenophora tetraphylla 45 weight portion～55 weight portions, Chinese gall 40 weight portions～50 weight portions, Kaempferia galanga 25 weight portion～35 weight portions, wilsonii 30 weight portion～40 weight portions, largeGarlic 15 weight portion～25 weight portions, nutmeg 30 weight portion～40 weight portions, radish seed 20 weight portions～30 weight portions, Java brucea 25 weight portion～35 weight portions, the wind-weed 40 weight portion～50 weight portions, femaleLoyal sub 45 weight portion～55 weight portions, hawthorn 30 weight portion～40 weight portions, Rose Mallow Root 25 weight portions～35 weight portions, sealwort 45 weight portion～55 weight portions, sensitive plant 30 weight portion～40 weight portions, partyJoin 45 weight portion～55 weight portions, kamuning 20 weight portion～30 weight portions, the fruit of Chinese wolfberry 35 weight portions～45 weight portions, perilla seed 20 weight portion～30 weight portions and cordate houttuynia 25 weight portion～35 weight portions.
Wherein, in described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 78 weight portion～82Weight portion, the Chinese bulbul 38 weight portion～42 weight portions, hairyvein agrimony 28 weight portion～32 weight portions, China ink droughtLotus 43 weight portion～47 weight portions, barrenwort 28 weight portion～32 weight portions, aleppo avens 48 weight portions～52 weight portions, the coptis 58 weight portion～62 weight portions, Samson St Johswort 33 weight portion～37 weight portions, southThe root of straight ladybell 48 weight portion～52 weight portions, Chinese gall 43 weight portion～47 weight portions, Kaempferia galanga 28 weight portions～32 weight portions, wilsonii 33 weight portion～37 weight portions, garlic 18 weight portion～22 weight portions, meatCardamom 33 weight portion～37 weight portions, radish seed 23 weight portion～27 weight portions, Java brucea 28 weightPart～32 weight portions, the wind-weed 43 weight portion～47 weight portions, the fruit of glossy privet 48 weight portion～52 weight portions,Hawthorn 33 weight portion～37 weight portions, Rose Mallow Root 28 weight portion～32 weight portions, sealwort 48 weight portions～52 weight portions, sensitive plant 33 weight portion～37 weight portions, Radix Codonopsis 48 weight portion～52 weight portions, nineIn fragrant 23 weight portion～27 weight portions, the fruit of Chinese wolfberry 38 weight portion～42 weight portions, perilla seed 23 weightPart～27 weight portions and cordate houttuynia 28 weight portion～32 weight portions.
Wherein, in described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 80 weight portions, whiteFather-in-law's 40 weight portions, hairyvein agrimony 30 weight portions, eclipta 45 weight portions, barrenwort 30 weight portions,Aleppo avens 50 weight portions, the coptis 60 weight portions, Samson St Johswort 35 weight portions, adenophora tetraphylla 50 weight portions,Chinese gall 45 weight portions, Kaempferia galanga 30 weight portions, wilsonii 35 weight portions, garlic 20 weight portions, meatCardamom 35 weight portions, radish seed 25 weight portions, Java brucea 30 weight portions, the wind-weed 45 weight portions, femaleLoyal sub 50 weight portions, hawthorn 35 weight portions, Rose Mallow Root 30 weight portions, sealwort 50 weight portions, bashfullyGrass 35 weight portions, Radix Codonopsis 50 weight portions, kamuning 25 weight portions, the fruit of Chinese wolfberry 40 weight portions, purple perillaSon 25 weight portions and cordate houttuynia 30 weight portions.
The present invention also provides the preparation method of above-mentioned Chinese medicine immune formulation, is specially:
The first step, by the Radix Astragali, eclipta, barrenwort, aleppo avens, adenophora tetraphylla, Chinese gall, wilsonii,The fruit of glossy privet, sealwort, Radix Codonopsis and the fruit of Chinese wolfberry mix in proportion, get mixture 100g and pack in percolator, addEnter the white wine 600g～800g of 50 DEG C～55 DEG C, under the condition of 25 DEG C～35 DEG C, soak 20 hours～30 littleTime, regulate percolator valve, white wine liquid is slowly oozed, the speed of oozing is 2～4mL per minute,Collect drip, obtain the percolate of 700mL～1L, concentrated, remove white wine, dry, pulverize, obtainThe first dried powder;
Second step, mixes component under She in proportion, gets 100g mixture, adds 500g～800g'sWater, boils 3～4 hours, filters, and obtains the first filtrate, and filter residue adds the water of 200g～300g again,Again boil 1～2 hour, filter, obtain the second filtrate, the first filtrate and the second filtrate are mixed, denseContracting, except anhydrating, dry, pulverize, and obtains the second dried powder;
The 3rd step, mixes the first dried powder and the second dried powder, obtains Chinese medicine immune formulation powder.
The present invention also provides a kind of chicken feed, and its raw material comprises corn, dregs of beans, salt, fish meal, bonePowder, trace mineral supplement, amino acid additive, vitamin(e) supplement and for preventing avian infectious methodThe Chinese medicine immune formulation powder feed addictive of family name's capsule.
Wherein, in described feed, the weight portion of each material composition is respectively corn 90 weight portion～110 weightsAmount part, dregs of beans 30 weight portion～50 weight portions, salt 2 weight portion～4 weight portions, fish meal 8 weight portions～12 weight portions, bone meal 3 weight portion～5 weight portions, trace mineral supplement 2 weight portion～4 weight portions,Amino acid additive 2 weight portion～4 weight portions, vitamin(e) supplement 2 weight portion～4 weight portion and Chinese medicinesImmune formulation powder feed addictive 15 weight portion～20 weight portions.
Wherein, in described feed, the weight portion of each material composition is respectively corn 100 weight portions, dregs of beans40 weight portions, salt 3 weight portions, fish meal 10 weight portions, bone meal 4 weight portions, trace element addAgent 3 weight portions, amino acid additive 3 weight portions, vitamin(e) supplement 3 weight portions and Chinese medicine immunity systemAgent powder feed addictive 18 weight portions.
The present invention also provides the preparation method of above-mentioned feed, is specially:
The first step, prepares described Chinese medicine immune formulation powder feed addictive according to the method described above;
Second step, by the described Chinese medicine immune formulation of other raw material components in feedstuff and first step acquisitionPowder feed addictive is put into fodder mixing machine together, and continuous uniform stirred after 1～3 hour, put into powderIn broken machine, pulverize, obtain feed.
Beneficial effect of the present invention:
Chinese medicine immune formulation provided by the invention has significant effect aspect prevention infections chicken cloacal bursaReally, and have nontoxic, the advantage of resistance, drug residue free.
Detailed description of the invention
The invention provides a kind of for preventing the Chinese medicine immune formulation of infections chicken cloacal bursa, its bulk drugComprise the Radix Astragali, the Chinese bulbul, hairyvein agrimony, eclipta, barrenwort, aleppo avens, the coptis, Samson St Johswort, southThe root of straight ladybell, Chinese gall, Kaempferia galanga, wilsonii, garlic, nutmeg, radish seed, Java brucea, the wind-weed, femaleLoyal son, hawthorn, Rose Mallow Root, sealwort, sensitive plant, Radix Codonopsis, kamuning, the fruit of Chinese wolfberry, perilla seed and fishRaw meat grass.
Further, described Chinese medicine immune formulation is only made up of above-mentioned raw materials medicine.
Fang Zhong, the Radix Astragali, aleppo avens can significantly improve cellular immunity and the humoral immunity ability of body, improveImmunologic function with adjusting body, suppresses infectious bursa of Fabricius virus infected chicken embryo fibroblast effectively,The Chinese bulbul, hairyvein agrimony have clearing heat and detoxicating and can improve macrophage phagocytic ability, and eclipta has heat-clearingEnriching yin and have cardiac stimulant anastalsis, barrenwort can obviously improve spleen antibody generation level, strengthens T lymphThe function of cell, promotes hyperplasia and the conversion of bone-marrow-derived lymphocyte, improves immunologic function, coptis energy anti-inflammatory,Antiallergic action, and have very wide antimicrobial spectrum, has and pretends use multiple germ and influenza virus,Samson St Johswort removing pattogenic heat from the blood and toxic material from the body, promoting blood circulation for regulating menstruation, adenophora tetraphylla can be improved cellular immunity and non-specific immunity, presses downHumoral immunity processed, has the function that regulates immunologic balance, astringent or styptic treatment for spontaneous sweating, the nourishing generate fluid of Chinese gall convergence, thorn fiveAdd and replenish qi to invigorate the spleen, tonify the kidney to relieve mental strain, in garlic, Kaempferia galanga temperature, help digestion, stomach is warmed up in promoting the circulation of qi, nutmeg, radishPuckery intestines, promote qi circulation digestion promoting in sub-temperature, Java brucea is clearing heat and detoxicating, and the wind-weed is nourshing Yin and drynsessmoistening prescription, removing heat from the lung to relieve cough,The fruit of glossy privet can promote leucocyte, strengthens effect of immunologic function, hawthorn digesting stomach invigorating, the Rose Mallow Root profit of dispeling the windWet, clearing heat and detoxicating, the sealwort yin-nourishing of fostering the spirit of nobility, invigorating the spleen moistening lung, sensitive plant removing pattogenic heat from the blood and toxic material from the body, clearing heat and promoting diuresis,Radix Codonopsis tonifying Qi benefit spleen, nourishing blood to promote the production of body fluid, kamuning promoting qi circulation and relieving pain, promoting blood circulation to remove blood stasis, the fruit of Chinese wolfberry tonifies the liver and kidney,Nourish blood and enrich blood, perilla seed sending down abnormally ascending dissolving phlegm, relieving cough and asthma, improves immunity of organisms, and cordate houttuynia is clearing heat and detoxicating,Removing pus and relieving carbuncle, all medicines share, and play altogether the merit of tonifying Qi enriching yin, clearing heat and detoxicating, promoting blood circulation, can regulateThe immunity of body, by heat-clearing antipyretic and antidote functions and subsidy to deficiency of vial QI, support, promotion etc.Effect, the cell factor can activate, promote, rise vigor being low, to adjust Molecular biological function,There is the effect that strengthens animal body immunity.
The pharmacology of each bulk drug is as follows:
The Radix Astragali: taste is sweet, warm in nature, enter spleen, lung channel, there is help spleen soil, elevate a turnable ladder yang-energy, strengthening exterior and reducing sweat,The effects such as holder sore myogenic, are mainly used in the weak anorexia and loose stool due to spleen-lung Qi deficiency, palpitation, middle gasThe visceral organ ptosis sinking, Qi dysfunction in blood control uterine bleeding is had blood in stool, and rushes down for a long time prolapse of the anus; The spontaneous sweating of exterior deficiency,The cards such as insufficiency of vital energy and blood. The Radix Astragali can increase cellular immunity and humoral immune function, increase IgM in blood plasma,IgA, has the effect of bidirectional modulation to immunologic function.
The Chinese bulbul: bitter, cold in nature, enter stomach, large intestine channel, have clearing heat and detoxicating, the effect of removing heat from the blood and relieve diarrhea,Being mainly used in stomach and intestine has damp and hot stagnant heating stomachache, diarrhea purulence blood, the tenesmus card that waits; To large intestine bloodBlood better effects if under the hot dysentery of heat, to shigella dysenteriae, typhoid bacillus, hay bacillus, Escherichia coli, HuangGold-coloured staphylococci has inhibitory action.
Hairyvein agrimony: latin name AgrimoniaeHerba, bitter, puckery, property is flat, the thoughts of returning home, Liver Channel,Have astringing to arrest bleeding, only effect of dysentery desinsection, cures mainly various hemorrhage cards, for example: spit blood, urinateBlood, have blood in stool, uterine bleeding, spitting of blood, bleeding from five sense organs or subcutaneous tissue, dysentery, internal lesion caused by overexertion takes off power, carbuncle is swollen, falls and beats, woundHinder the hemorrhage card of Denging
Eclipta: have another name called Eclipta prostrata, China ink grass, floods and droughts lotus, taste is sweet, sour, cold in nature, returns liver, kidney channel,Play and tonify the liver and kidney, the effect of cooling blood and hemostasis, for having a dizzy spell due to the deficiency of liver-yin and kidney-yin, blurring of vision,Soreness and weakness of waist and knees; Being suitable for the deficiency of Yin has hot person, is used for the treatment of the haematemesis of accumulated heat in stomach, the hemoptysis of heat impairing pulmonary collateralEtc. symptom, eclipta has staphylococcus aureus, typhoid bacillus, Song's Shigella flexneri, Pseudomonas aeruginosaInhibitory action, can obviously strengthen nospecific immunity and cellular immune function.
Barrenwort: latin name EpimediiFolium, taste is pungent, sweet, warm in nature, enters liver, kidney channel, toolThere is kidney-replenishing, strengthening the bones and muscles, effect of wines used as antirheumatic, cures mainly impotence due to deficiency of the kidney, seminal emission premature ejaculation, waist knee impotenceSoft, cold extremities with aversion to cold; The cards such as numbness and ache of cold dampness or spasm of limbs are numb, barrenwort is traditional Bu Shen ZhuangSun Chinese medicine, is rich in flavone compound, has gonadotropic Effect, directly suppresses bone and absorbs and urgeEnter bone and form, make organism metabolism form and be greater than the positive balance state that bone absorbs in bone.
Aleppo avens: latin name GeiHerba, taste is pungent, bitter, and property is flat, returns liver, spleen, lung channel, hasAnalgesia, step-down, menstruation regulating, the effect of dispelling rheumatism, cures mainly high blood pressure, dizziness headache, menstruationUncomfortable, the pain of lower abdomen, leukorrhea, child convulsion, lumbago, skelalgia of rheumatism; Carbuncle furuncle poison is controlled in external application, falls and beatsThe cards such as damage.
The coptis: bitter, cold in nature, enter the heart, lung, courage, stomach, large intestine channel, there is heat-clearing and damp-drying drug, purging intense heat solutionPoison, the effect of cooling blood for improving eyesight, is usually used in treating the fire of removing the heart-fire warp, the dysphoria and insomnia that internal heat is prosperous, and warmThe high fever coma of disease, the card such as vexed, thirsty.
Samson St Johswort: bitter, pungent, cold in nature, return liver, the spleen channel, there is removing pattogenic heat from the blood and toxic material from the body, promoting blood circulation for regulating menstruation, dispelsEffect of wind and removing obstruction in channels, cure mainly various hemorrhage, enteritis and dysentery, acute mastitis, the carbuncle serious case of furuncle that swells, scalds, snake bite,Irregular menstruation, dysmenorrhoea, leukorrhea, traumatic injury, arthralgia due to wind-dampness, the cards such as aphtha.
Adenophora tetraphylla: sweet, the micro-hardship of taste, cold nature, enters lung, stomach warp, moistens the lung and relieve the cough, nourishing the stomach to improve the production of body fluidEffect, is mainly used in the few phlegm of the lung-dryness syndrome due to yin deficiency, dry cough, the labor phlegm blood of coughing, and deficiency of stomach-Yin, mouthfeel pharynx are dry, largeThe cards such as constipation knot, the red few tongue of tongue, can improve cellular immunity and non-specific immunity, suppress humoral immunity,There is the function that regulates immunologic balance.
Chinese gall: latin name GallaChinensis, taste acid, puckery, cold in nature, return lung, large intestine, kidney channel,The effect with astringent or styptic treatment for spontaneous sweating, the nourishing generate fluid of convergence, kidney calming, cures mainly: chronic cough of deficiency lung; Spontaneous sweating;Protracted dysentery is rushed down for a long time; Prolapse of the anus; Seminal emission; Gonorrhoea; Various hemorrhage; The cards such as the swollen sore furuncle of carbuncle, Chinese gall energy enhanced machineThe defence capability of body to nonspecific stimulation, can strengthen the immunologic function of cell, and this pathogenic bacteria are had and pressed downMake use, and can antivirally grow.
Kaempferia galanga: taste is pungent, warm in nature, returns stomach warp, has in promoting the circulation of qi temperature, and effect of the pain relieving that helps digestion, cures mainly chestDiaphragm turgor, coldness and pain in the epigastrium, the diet card such as do not disappear.
Wilsonii: latin name AcanthopanacisSenticosiRadixetRhizomaseuCaulis,Taste is pungent, bitter, micro-sweet, warm in nature, enters spleen, kidney, the heart channel of Hang-Shaoyin, has and replenishes qi to invigorate the spleen, and tonifies the kidney to relieve mental strain, and dispels the windEffect of dehumidifying, soft for wind-cold-dampness arthralgia, lumbocrural pain, muscles and bones impotence, be slow in action, body void is thin and weak,Traumatic injury, fracture, oedema, beriberi, the uncontrolled urination card such as itch.
Garlic: warm in nature, taste is pungent flat; Enter spleen, stomach, lung channel, have in temperature help digestion, row stagnant gas,Effect of warming spleen and stomach, long-pending, the removing toxic substances of disappearing, desinsection, cures mainly eating accumulation, coldness and pain in the epigastrium, oedema is swollenExpire, have loose bowels, dysentery, malaria, pertussis, swollen ulcer drug, white bald tinea sore, snake bite and insect sting andThe illness such as hookworm, pinworm.
Nutmeg: latin name MyristicaeSemen, taste is pungent, bitter, warm in nature, enters spleen, stomach, large intestineWarp, has the effect of puckery intestines, promote qi circulation digestion promoting in temperature, for void rush down, cold dysentery, abdominal distention, foodFew vomiting, the place food card such as do not disappear.
Radish seed: property is flat, and taste is pungent, sweet, returns lung, spleen, stomach warp, has the de-swelling of helping digestion, lowering the adverse-rising QI to resolve phlegmEffect, cure mainly retention of food and drink, abdominal distention, constipation, the stagnant dysentery, phlegm of rushing down is stopped up the card such as breath with cough.
Java brucea: latin name BruceaeFructus, bitter, cold in nature, return large intestine channel, Liver Channel, haveClearing heat and detoxicating, only dysentery, preventing malaria, effect of corrosion wart, cures mainly dysentery, malaria; Control wart, chicken outwardThe cards such as eye.
The wind-weed: latin name AnemarrhenaeRhizoma, bitter, sweet, cold in nature, enter lung, stomach, kidneyWarp, has the relieving restlessness of purging heat, and the effect of nourshing Yin and drynsessmoistening prescription, removing heat from the lung to relieve cough, cures mainly fever caused by exogenous pathogenic factors, and high heat is irritated,Lung-heat type cough, the interior heat symptom such as quench one's thirst; Its bitter cold matter profit, property sedimentation, can heat-clearing, can promote the production of body fluid again,Kind control warm disease and evil and enter gas and divide, heat is contained and is hindered Tianjin; Also be used for the treatment of diabetes; Phlegm-heat obstructing lung and breathe heavily the dryness of the lungCough, phlegm is few unfavorable; Fire excess from yin deficiency, the symptoms such as flaring up of deficient fire, the wind-weed to shigella dysenteriae, typhoid bacillus,The various bacteria such as Escherichia coli, proteus, staphylococcus, streptococcus have inhibitory action.
The fruit of glossy privet: taste is sweet, bitter, is slightly cold, enters liver, kidney channel, has strong liver kidney-nourishing, the effect of removing heat to brighten vision,Be mainly used in treating liver-kidney Yin deficiency, the head loses foster dizzy tinnitus, the symptoms such as yin asthenia generating intrinsic heat. Fruit of glossy privet energyPromote leucocyte, strengthen effect of immunologic function, have stronger to staphylococcus aureus, shigella dysenteriaeInhibitory action. Modern medicine study proves, the fruit of glossy privet include ursolic acid, sweet mellow wine, glucose,Fat wet goods, has cardiac stimulant, diuresis and hepatoprotective effect, can make the leucocyte decline number causing because of chemotherapyAmount raises, and staphylococcus aureus, Escherichia coli are had to inhibitory action.
Hawthorn: taste acid, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, has food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apartEffect, be mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, hawthorn for treatment indigestion conventionalProduct, hawthorn can increase the secretion of peptic digest enzyme, promotes the cards such as digestion.
Rose Mallow Root: taste is sweet, light, property is flat, enters the heart, lung channel, has expelling wind and removing dampness, clearing heat and detoxicating meritEffect, cures mainly cold, fever, arthralgia due to wind-dampness, and dysentery, oedema, leukorrhea, spits blood, and carbuncle is swollen, traumatism and bleeding,Venomous snake bite, acute infantile convulsions, lockjaw, asthma, the cards such as stomachache cold after delivery.
Sealwort: latin name PolygonatiRhizoma, taste is sweet, and property is flat, enters lung, spleen, kidney channel, toolThe yin-nourishing of fostering the spirit of nobility, invigorating the spleen moistening lung, effect of enriching kidney essence, for taste deficiency of qi and yin, the few nothing of foodPower, the lung-dryness syndrome due to yin deficiency, the few phlegm of dry cough, the quenching one's thirst of yin asthenia generating intrinsic heat, deficiency of kidney essence, impotence and seminal emission, headThe cards such as dizzy tinnitus, blurred vision dim eyesight.
Sensitive plant: taste is sweet, puckery, micro-hardship, cold nature, returns spleen, kidney two warps, has removing pattogenic heat from the blood and toxic material from the body, clearHot dampness removing, effect of tranquilizing and allaying excitement, cures mainly flu, infantile hyperpyrexia, bronchitis, hepatitis, gastritis,Conjunctivitis, stone in urinary system, oedema, hemoptysis, blood urine, neurasthenia, insomnia, sore swollen toxin, bandShape bleb, the cards such as traumatic injury.
Radix Codonopsis: taste is sweet, property is flat, returns lung, the spleen channel, has tonifying Qi benefit spleen, and effect of nourishing blood to promote the production of body fluid is mainControl spleen-lung Qi deficiency or qi-blood deficiency lassitude hypodynamia, breathe hard, the symptoms such as cough spontaneous perspiration, Radix Codonopsis is longer than tonifying spleenNourishing the stomach, adjusts regulating the function of middle-JIAO, has the effect of nourishing blood concurrently, and its property is flat, invigorating the spleen fortune and not dry; Flavour is cloudy and not wet,Can improve the immune state of body, improve resistance against diseases, promote to digest and assimilate, improve metabolism,Promote the absorption of enteron aisle to nutriment.
Kamuning: pungent, the micro-hardship of taste, warm in nature, return liver, stomach warp, has promoting qi circulation and relieving pain, promoting blood circulation to remove blood stasisEffect, cures mainly stomachache, arthralgia due to wind-dampness; Control toothache outward, tumbling and swelling, the cards such as worm snake bite.
The fruit of Chinese wolfberry: taste is sweet, property is flat, enters liver, kidney channel, tonify the liver and kidney, nourish blood enrich blood, benefiting shrewd head,The effect moistening the lung and relieve the cough, is mainly used in liver kidney two void, the soreness and weakness of waist and knees of asthenia of essence and blood, dizziness and tinnitus, something lostEssence tinnitus, unconsciously sweat and tire; The impotence of the insufficiency of the kidney yang, sterile; The eyes dusk of deficiency of YIN-essence,impairment of YIN-essence, deficiency of liver-bloodFlower and deficiency of Yin labor cough wait card, the fruit of Chinese wolfberry can promote liver cell regeneration, enhancing non-specific immune functionEffect.
Perilla seed: latin name PerillaeFructus, the dry mature fruit that perilla seed is purple perilla, its tastePungent, warm in nature, nontoxic, return lung channel, there is sending down abnormally ascending dissolving phlegm, relieving cough and asthma, effect of relaxing bowel, mainControl phlegm and stop up the circulation of vital energy in the wrong direction, cough and asthma, the cards such as the dry constipation of intestines, the main active component in perilla seed is ω-3Polyunsaturated fatty acid (ω-3PUFA), ω-3PUFA and flavones have specific biology to livestock and poultryActive function. The content that increases ω-3PUFA in diet can improve ω-3PUFA's in livestock productsContent, polyunsaturated fatty acid (PUFA) and saturated fatty acid (SFA) ratio, ω-3PUFA withOmega 6 polyunsaturated fatty acid (ω-6PUFA) ratio, is conducive to regulate immunologic function and slows down inflammation anti-Should.
Cordate houttuynia: taste is pungent, cold nature, enters lung, urinary bladder channel, have clearing heat and detoxicating, removing pus and relieving carbuncle, profitUrinate treating stranguria effect, for lung carbuncle pyemesis, phlegm heat is coughed and is breathed heavily, toxic-heat and blood stasis, and damp-heat gonorrhoea, hot carbuncle carbuncle,Snakeworm is wanted first-class card, and cordate houttuynia master contains decanoyl acetaldehyde, orange osmanthus aldehyde, methylnonanone, Bai Pili glucoside etc.,Major function is swelling and pain relieving, hemostasis, antibacterial, and can strengthen immunity of organisms, improves local blood and followsRing, reduces inflammatory and oozes out, and promotes epithelial repair.
In described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively:
The Radix Astragali 75 weight portion～85 weight portions, the Chinese bulbul 35 weight portion～45 weight portions, hairyvein agrimony 25Weight portion～35 weight portion, eclipta 40 weight portion～50 weight portions, barrenwort 25 weight portion～35Weight portion, aleppo avens 45 weight portion～55 weight portions, the coptis 55 weight portion～65 weight portions, Samson St Johswort30 weight portion～40 weight portions, adenophora tetraphylla 45 weight portion～55 weight portions, Chinese gall 40 weight portions～50 weight portions, Kaempferia galanga 25 weight portion～35 weight portions, wilsonii 30 weight portion～40 weight portions, largeGarlic 15 weight portion～25 weight portions, nutmeg 30 weight portion～40 weight portions, radish seed 20 weight portions～30 weight portions, Java brucea 25 weight portion～35 weight portions, the wind-weed 40 weight portion～50 weight portions, femaleLoyal sub 45 weight portion～55 weight portions, hawthorn 30 weight portion～40 weight portions, Rose Mallow Root 25 weight portions～35 weight portions, sealwort 45 weight portion～55 weight portions, sensitive plant 30 weight portion～40 weight portions, partyJoin 45 weight portion～55 weight portions, kamuning 20 weight portion～30 weight portions, the fruit of Chinese wolfberry 35 weight portions～45 weight portions, perilla seed 20 weight portion～30 weight portions and cordate houttuynia 25 weight portion～35 weight portions.
Further preferably, in described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 78 weightPart～82 weight portions, the Chinese bulbul 38 weight portion～42 weight portions, hairyvein agrimony 28 weight portion～32 weightPart, eclipta 43 weight portion～47 weight portions, barrenwort 28 weight portion～32 weight portions, aleppo avens48 weight portion～52 weight portions, the coptis 58 weight portion～62 weight portions, Samson St Johswort 33 weight portion～37Weight portion, adenophora tetraphylla 48 weight portion～52 weight portions, Chinese gall 43 weight portion～47 weight portions, Kaempferia galanga28 weight portion～32 weight portions, wilsonii 33 weight portion～37 weight portions, garlic 18 weight portion～22Weight portion, nutmeg 33 weight portion～37 weight portions, radish seed 23 weight portion～27 weight portions, crow courageSon 28 weight portion～32 weight portions, the wind-weed 43 weight portion～47 weight portions, the fruit of glossy privet 48 weight portions～52 weight portions, hawthorn 33 weight portion～37 weight portions, Rose Mallow Root 28 weight portion～32 weight portions, HuangEssence 48 weight portion～52 weight portions, sensitive plant 33 weight portion～37 weight portions, Radix Codonopsis 48 weight portions～52 weight portions, kamuning 23 weight portion～27 weight portions, the fruit of Chinese wolfberry 38 weight portion～42 weight portions,Perilla seed 23 weight portion～27 weight portions and cordate houttuynia 28 weight portion～32 weight portions.
Further preferred, in described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 80Weight portion, the Chinese bulbul 40 weight portions, hairyvein agrimony 30 weight portions, eclipta 45 weight portions, barrenwort30 weight portions, aleppo avens 50 weight portions, the coptis 60 weight portions, Samson St Johswort 35 weight portions, adenophora tetraphylla50 weight portions, Chinese gall 45 weight portions, Kaempferia galanga 30 weight portions, wilsonii 35 weight portions, garlic 20Weight portion, nutmeg 35 weight portions, radish seed 25 weight portions, Java brucea 30 weight portions, the wind-weed 45Weight portion, the fruit of glossy privet 50 weight portions, hawthorn 35 weight portions, Rose Mallow Root 30 weight portions, sealwort 50 weightsAmount part, sensitive plant 35 weight portions, Radix Codonopsis 50 weight portions, kamuning 25 weight portions, the fruit of Chinese wolfberry 40 weightsAmount part, perilla seed 25 weight portions and cordate houttuynia 30 weight portions.
Chinese medicine immune formulation provided by the invention is prepared into powder, mix in feed, the ill chicken of feeding.
The present invention also provides the preparation method of above-mentioned Chinese medicine immune formulation powder, is specially:
The first step, by the Radix Astragali, eclipta, barrenwort, aleppo avens, adenophora tetraphylla, Chinese gall, wilsonii,The fruit of glossy privet, sealwort, Radix Codonopsis and the fruit of Chinese wolfberry mix in proportion, get mixture 100g and pack in percolator, addEnter the white wine 600g～800g of 50 DEG C～55 DEG C, under the condition of 25 DEG C～35 DEG C, soak 20 hours～30 littleTime, regulate percolator valve, white wine liquid is slowly oozed, the speed of oozing is 2～4mL per minute,Collect drip, obtain the percolate of 700mL～1L, concentrated, remove white wine, dry, pulverize, obtainThe first dried powder;
Second step, mixes component under She in proportion, gets 100g mixture, adds 500g～800g'sWater, boils 3～4 hours, filters, and obtains the first filtrate, and filter residue adds the water of 200g～300g again,Again boil 1～2 hour, filter, obtain the second filtrate, the first filtrate and the second filtrate are mixed, denseContracting, except anhydrating, dry, pulverize, and obtains the second dried powder;
The 3rd step, mixes the first dried powder and the second dried powder, obtains Chinese medicine immune formulation powder.
The present invention also provides a kind of chicken feed, and its raw material comprises corn, dregs of beans, salt, fish meal, bonePowder, trace mineral supplement, amino acid additive, vitamin(e) supplement and for preventing avian infectious methodThe Chinese medicine immune formulation powder feed addictive of family name's capsule.
Wherein, in described feed, the weight portion of each material composition is respectively corn 90 weight portion～110 weightsAmount part, dregs of beans 30 weight portion～50 weight portions, salt 2 weight portion～4 weight portions, fish meal 8 weight portions～12 weight portions, bone meal 3 weight portion～5 weight portions, trace mineral supplement 2 weight portion～4 weight portions,Amino acid additive 2 weight portion～4 weight portions, vitamin(e) supplement 2 weight portion～4 weight portion and Chinese medicinesImmune formulation powder feed addictive 15 weight portion～20 weight portions.
Further preferably, in described feed, the weight portion of each material composition be respectively corn 100 weight portions,Dregs of beans 40 weight portions, salt 3 weight portions, fish meal 10 weight portions, bone meal 4 weight portions, trace elementAdditive 3 weight portions, amino acid additive 3 weight portions, vitamin(e) supplement 3 weight portions and Chinese medicine are exempted fromEpidemic disease preparation powder feed addictive 18 weight portions.
Trace mineral supplement can be the trace mineral supplement of various routines, can use simply and haveMachine hydrochlorate, as ironic citrate, also can use trace element and amino-acid chelate, as amino acid iron,The trace element adding mainly contains iron, zinc, copper, manganese, iodine, cobalt, selenium, molybdenum, also can use compoundMicroelement pre-mix material, the Great Britain and America who provides as Beijing You Libao biotechnology Co., Ltd you.
Amino acid additive comprises serine, glutamic acid, glycine, isoleucine, leucine, the third ammoniaThe various conventional amino acid such as acid, proline, also can adopt amino acid premix, as above sea cowry energy chemical industryThe composite aminophenol powder that Co., Ltd produces.
Vitamin(e) supplement comprises riboflavin, vitamin C, vitamin E, vitamin A, CobastabEtc. various conventional vitamin, also can adopt vitamin premix, it is limited that profit as excellent in Beijing is protected biotechnologyThe excellent dimension element that company produces.
The present invention also provides the preparation method of above-mentioned feed, is specially:
The first step, prepares described Chinese medicine immune formulation powder feed addictive according to the method described above;
Second step, by the described Chinese medicine immune formulation of other raw material components in feedstuff and first step acquisitionPowder feed addictive is put into fodder mixing machine together, and continuous uniform stirred after 1～3 hour, put into powderIn broken machine, pulverize, obtain feed.
Below adopt embodiment to describe embodiments of the present invention in detail, whereby how the present invention is appliedTechnological means solves technical problem, and the implementation procedure of reaching technique effect can fully understand and real according to thisExecute.
Embodiment 1 Chinese medicine immune formulation 1
By Radix Astragali 80g, eclipta 45g, barrenwort 30g, aleppo avens 50g, adenophora tetraphylla 50g, five timesSub-45g, wilsonii 35g, fruit of glossy privet 50g, sealwort 50g, Radix Codonopsis 50g and fruit of Chinese wolfberry 40g are in proportionMix, get mixture 100g and pack in percolator, add the white wine 700g of 50 DEG C, the condition of 30 DEG CLower immersion 25 hours, regulates percolator valve, and white wine liquid is slowly oozed, and the speed of oozing is per minute3mL, collects drip, obtains the percolate of 800mL, concentrated, removes white wine, dry, pulverize, and obtainsObtain the first dried powder, by Chinese bulbul 40g, hairyvein agrimony 30g, coptis 60g, Samson St Johswort 35g, Kaempferia galanga30g, garlic 20g, nutmeg 35g, radish seed 25g, Java brucea 30g, wind-weed 45g, hawthorn 35g,Rose Mallow Root 30g, sensitive plant 35g, kamuning 25g, perilla seed 25g and cordate houttuynia 30g mix, and get 100gMixture, adds the water of 600g, boils 4 hours, filters, and obtains the first filtrate, and filter residue adds againThe water of 300g, boils 2 hours again, filters, and obtains the second filtrate, by the first filtrate and the second filtrateMix, concentrated, except anhydrating, dry, pulverize, obtain the second dried powder, by the first dried powder andThe second dried powder mixes, and obtains Chinese medicine immune formulation 1.
Embodiment 2 Chinese medicine immune formulations 2
By Radix Astragali 78g, eclipta 43g, barrenwort 28g, aleppo avens 48g, adenophora tetraphylla 48g, five timesSub-43g, wilsonii 33g, fruit of glossy privet 48g, sealwort 48g, Radix Codonopsis 48g and fruit of Chinese wolfberry 38g are in proportionMix, get mixture 100g and pack in percolator, add the white wine 700g of 50 DEG C, the condition of 30 DEG CLower immersion 25 hours, regulates percolator valve, and white wine liquid is slowly oozed, and the speed of oozing is per minute3mL, collects drip, obtains the percolate of 800mL, concentrated, removes white wine, dry, pulverize, and obtainsObtain the first dried powder, by Chinese bulbul 38g, hairyvein agrimony 28g, coptis 58g, Samson St Johswort 33g, Kaempferia galanga28g, garlic 18g, nutmeg 33g, radish seed 23g, Java brucea 28g, wind-weed 43g, hawthorn 33g,Rose Mallow Root 28g, sensitive plant 33g, kamuning 23g, perilla seed 23g and cordate houttuynia 28g mix, and get 100gMixture, adds the water of 600g, boils 4 hours, filters, and obtains the first filtrate, and filter residue adds againThe water of 300g, boils 2 hours again, filters, and obtains the second filtrate, by the first filtrate and the second filtrateMix, concentrated, except anhydrating, dry, pulverize, obtain the second dried powder, by the first dried powder andThe second dried powder mixes, and obtains Chinese medicine immune formulation 2.
Embodiment 3 Chinese medicine immune formulations 3
By Radix Astragali 82g, eclipta 47g, barrenwort 32g, aleppo avens 52g, adenophora tetraphylla 52g, five timesSub-47g, wilsonii 37g, fruit of glossy privet 52g, sealwort 52g, Radix Codonopsis 52g and fruit of Chinese wolfberry 42g are in proportionMix, get mixture 100g and pack in percolator, add the white wine 700g of 50 DEG C, the condition of 30 DEG CLower immersion 25 hours, regulates percolator valve, and white wine liquid is slowly oozed, and the speed of oozing is per minute3mL, collects drip, obtains the percolate of 800mL, concentrated, removes white wine, dry, pulverize, and obtainsObtain the first dried powder, by Chinese bulbul 42g, hairyvein agrimony 32g, coptis 62g, Samson St Johswort 37g, Kaempferia galanga32g, garlic 22g, nutmeg 37g, radish seed 27g, Java brucea 32g, wind-weed 47g, hawthorn 37g,Rose Mallow Root 32g, sensitive plant 37g, kamuning 27g, perilla seed 27g and cordate houttuynia 32g mix, and get 100gMixture, adds the water of 600g, boils 4 hours, filters, and obtains the first filtrate, and filter residue adds againThe water of 300g, boils 2 hours again, filters, and obtains the second filtrate, by the first filtrate and the second filtrateMix, concentrated, except anhydrating, dry, pulverize, obtain the second dried powder, by the first dried powder andThe second dried powder mixes, and obtains Chinese medicine immune formulation 3.
Embodiment 4 toxicological experiments
Acute toxicity testing
Select 20 of Kunming mouses, female half and half, body weight (20+2) g, in Qingdao City animal used as testThe heart provides, and Chinese medicine immune formulation prepared by embodiment 1 is dissolved in water, and obtaining crude drug concentration is 1g/mlSolution, gavage mouse, through preliminary experiment, fail to detect the LD of oral administration50Therefore, survey its maximumDosis tolerata, with the administration volume of 1.4ml/10g body weight, gavages 2 times/d, and interval 6h gives observation post administration7d, records animal dead and toxicity symptom, when experiment finishes, by all animals processing, dissects,Visually observe each main organs lesion tissue situation, gavage for 2 times after 140g crude drug/kg, animal activity,Feed water inlet does not show any abnormalities, and observes 7d animal dead, and gross necropsy has no the bright of main organs tissueAobvious abnormal, therefore, the maximum dosage-feeding of Chinese herbal granules is > 140g crude drug/kg, and this dosage is equivalent to faceBed adult's (by 60kg) every day 70 times of maximum oral dose 2g crude drug/kg.
Long term toxicity test
Select 40 male SD rats, weight (180 ± 20) g, is carried by Qingdao City's Experimental Animal CenterConfession, is divided into 2 groups at random, blank group and Chinese drug-treated group, and 20 every group, blank group is distilledWater, Chinese medicine immune formulation prepared by embodiment 1 is dissolved in water, and acquisition crude drug concentration is 1g/mlSolution, gavages rat, and the amount of gavaging is 50g crude drug/kg, on every Mondays to gastric infusion 1 time in morning Saturday,Continuously 180d, administration volume is 2mL/100g body weight, observe rat one every day before administration and during administrationAs state, sign, food ration, drinking-water and ight soil situation. When administration 90d after last administration 24h,Each group extracts 6, male and female half and half; When 180d, after last administration 24h, each group extracts 6, and male and female are eachHalf; After drug withdrawal, 30d respectively organizes and extracts 6, and male and female half and half, with urethane anesthesia, are got from abdominal aorticBlood, each group is all killed and gets animal, checks hematology and blood parameters, gets after blood, amass wealth by heavy taxation at once core,Liver,kidney,spleen, lung, adrenal gland, thyroid gland, thymus gland, testis, ovary, uterus, brain, prostateWeigh in internal organs, according to weight calculator official weight coefficient and make pathological examination.
On inspection, during successive administration, the activity of animal, body weight, food ration, stool and hair lightPool degree no abnormality seen, it is dead that animal has no, and naked eyes have no obvious pathology, respectively organize the inspection of rat peripheral hemogramLook into all in normal range (NR), physiochemical indice is all in normal range (NR), Chinese drug-treated group Rats Organs and Tissues coefficient and blankGroup is compared does not have significant difference.
Embodiment 5 feeds
By corn 10kg, dregs of beans 4kg, salt 0.3kg, fish meal 0.1kg, bone meal 0.4kg, trace elementAdditive Great Britain and America that (Beijing You Libao biotechnology Co., Ltd provides) 0.3kg, amino acid additiveComposite aminophenol powder (above sea cowry energy Chemical Co., Ltd. produces) 0.3kg, the excellent dimension element of vitamin(e) supplement (BeijingYou Libao Bioisystech Co., Ltd produces) the Chinese medicine immune formulation 1.8kg for preparing of 0.3kg and embodiment 1Put into together fodder mixing machine, continuous uniform stirred after 3 hours, and put into pulverizer and pulverize,Obtain feed.
Comparative example 1 feed
By corn 10kg, dregs of beans 4kg, salt 0.3kg, fish meal 0.1kg, bone meal 0.4kg, trace elementAdditive Great Britain and America that (Beijing You Libao biotechnology Co., Ltd provides) 0.3kg, amino acid additiveComposite aminophenol powder (above sea cowry energy Chemical Co., Ltd. produces) 0.3kg, the excellent dimension element of vitamin(e) supplement (BeijingYou Libao Bioisystech Co., Ltd produces) 0.3kg puts into fodder mixing machine together, and continuous uniform stirsAfter 3 hours, put into pulverizer and pulverize, obtain feed.
Embodiment 6 zooperies
Animal used as test
Select public young 150 of the nonimmune Luo Man of 1 age in days.
Experimental drug
Feed prepared by embodiment 5.
Attacking the preparation of poison chicken infectivity bursa of Fabricius virus and malicious valency measures
Get the bursa of farbricius that field acquisition has typical cytopathic, smash multigelation to pieces 4 times with tissue mashing machine,The centrifugal 10min of 800r/min, gets supernatant, She's slag again with normal saline dilution, centrifugal, suct clear liquid,4 times repeatedly, merge supernatant and make 1:4 homogenate ,-20 DEG C save backup. Get supernatant physiologySalt solution does 10-2～10-7Serial dilution, is inoculated in respectively 9 age in days SPF chicken embryos, and each dilution factor connectsPlant 5, every embryo 0.1mL; If physiological saline contrast. 37 DEG C of hatching 120h, add up each groupChicken embryo death number, calculating EID50 by Reach-Muench method is 10～7.25. Use before usePhysiological saline was with dilution in 1: 10.
Detection positive serum
Provided by Qingdao Agricultural University, tiring is 1: 26.
Experimental design
Select 150 of 1 Japanese instar chicklings, be divided at random blank group, attack malicious control group and prevention group,Every group 50. Take strict quarantine measures for each group, after separating, raise according to a conventional method, free choice feeding drinking-water,24h illumination, except Bursal Disease, normally immunity, feeding and management level is made every effort to unanimously, pre-Anti-group of feed prepared by the feeding embodiment of the present invention 5, while being fed to the 15th age in days, the poison contrast of attacking against each otherGroup and prevention group chick directly gavage chicken infectivity bursa of Fabricius virus, attack poison amount for every disease of 1: 10Poison dilution 2.0mL, blank group chick is applied 0.9% physiological saline and gavages 2.0mL, does not attack poison,Attack the feed of malicious control group feeding comparative example 1.
Duration of test, examines every day such as state of mind, appetite, ight soil, morbidity, death of chicken group etc.Situation, the death toll of each group of chick of record test, and the chick that dies of illness is cutd open to inspection by only carrying out pathology, detectAnd the situation such as hemorrhage of adding up the organs such as chest muscle, leg flesh, the bursa of farbricius.
Detect index and data processing
The mensuration of serum infections chicken cloacal bursa virus resisting antibody titer is attacked malicious latter 1 week to each group of test chicken5 of random extractions, venous blood collection under the wing, separation of serum, adopts semimicro blood clotting to suppress experimental method and measuresIBDV antibody titer in serum, HI antibody titer is with Log2 XRepresent.
The mensuration that chicken splenic lymphocytes transforms
Test after the full phase finishes, randomly draw 1 experimental chicken, jugular vein sacrificed by exsanguination, aseptic getting for every groupGo out spleen, rinse 3 times with sterile saline respectively, after putting into and shredding containing the ware of Hank ' s liquid,Proceed to again in the sterilizing nylon net bag of 100 order mesh, with curved needle head press filtration gently, collecting cell liquid,Carefully be added in lymphocyte separation medium upper strata, the centrifugal 20min of 2000r/min, in the middle of drawing, cloud is whiteCellular layer, washes 2 times the centrifugal 10min of 1500r/min with Hank ' s liquid. After viable count, containIt is 1 × 10 that the RPMI1640 nutrient solution of 10% calf serum is adjusted cell density7Individual/mL.
Adopt mtt assay, by separate 2 parts of each above-mentioned preparation group chicken splenic lymphocytes, add respectivelyConcanavaline (ConA) and bacteria lipopolysaccharide (LPS), making its mass concentration is 5 and 10mg/L.Subsequently, together with untreated chicken splenic lymphocytes suspension, be added to respectively in 96 porocyte culture plates,100 μ L/ holes, every group is repeated 4 holes. Put 39.5 DEG C, 5%CO2While cultivating 44h in incubator,Every hole adds MTT20 μ L continues to cultivate after 4h, sucking-off supernatant, and every hole adds DMSO100 μ L.Cell plates are put and on micro-oscillator, shaken 5min precipitation is dissolved completely, on enzyme linked immunological instrument, detectLight absorption value (D570), calculate lymphocyte transformation rate. Computing formula is:
Lymphocyte transformation rate (%)=(the average OD value of the average OD value-control wells of experimental port)/averageOD value × 100
Data processing and analysis
Each group data are all used mean soil standard deviationRepresent, respectively organize mean and do test of normality and repeat to surveyAmount homogeneity test of variance, younger inspection between organizing with one-way analysis of variance), P < 0.05 is for differing aobviousWork, P < 0.01 is for differing highly significant. Statistical computation all adopts SPSSl2.0 software to complete.
Result
Clinical symptoms
Each group chick is manually gavaging before chicken infectivity bursa of Fabricius virus, spirit, appetite and the excrement of chickJust etc. situation is normal. After the 15th age in days gavages, front 2d also has no obvious clinical symptoms, only minorityChick occurs that feed intake reduces, and indivedual chickens have slightly depressed. 3d after gavaging, attacks malicious control group and preventionGroup chicken starts to occur that spirit is depressed, and appetite obviously reduces, and disorderly, two wing powers are sagging for part chick feather pine,Necking down, closes the sleeping ground of order, and anus around feather has fecal pollution phenomenon, and some discharges white water sample loose stool,Sick chicken starts to occur to exhaust death condition because of dehydration. But clinical symptoms performance significant difference between two groups. In advanceThe clinical symptoms of anti-group of chick are significantly better than majoring in control group, and blank group is without obvious clinical symptoms.
Pathological change
The chicken that dies of illness is cutd open to inspection, find that the chicken that dies of illness is serious dehydration phenomenon, leg flesh is kermesinus or a littleShape, patch shape, sheet are hemorrhage, and chest muscle kermesinus is even point-like blood, and bursa of farbricius mucous membrane fold has different journeysThat spends is hemorrhage, and mucous membrane has oedema phenomenon, has gel-shaped mucus in blister cavities, or butyrous secretion, minorityIn chamber, have caseous substance, muscular stomach and glandular stomach intersection have the hemorrhage band of point-like and strip and block, minority glandular stomachMucosa ulcer, indivedual glandular stomach nipples have blutpunkte, and part chick enteron aisle has in various degree hemorrhage, rectumHemorrhage comparatively serious with cloaca, kidney enlargement is hemorrhage, on indivedual hearts are dirty, has hemorrhage situation.
Each group chick death condition, the results are shown in Table 1.
Table 1 is attacked the death condition comparison of the rear each group chicken of poison
As can be seen from Table 1, adopt Chinese medicine immune formulation of the present invention can significantly reduce infectionThe M & M of property bursal disease, stops death when prevention group to the 12 days, attacks malicious control groupStop death to the 15th old name for the Arabian countries in the Middle East, data difference heteropole is (P < 0.01) significantly.
The die of illness variation of chicken organ hemorrhage positive rate, the results are shown in Table 2.
The dead chicken pathology of the each group test of table 2 cuts open and detects blood positive rate (%)
| Hemorrhage organ | Prevention group | Attack malicious control group | Blank group |
| Chest muscle | 20 | 75 | 0 |
| Leg flesh | 22 | 76 | 0 |
| Spleen | 21 | 72 | 20 |
| The bursa of farbricius | 33 | 94 | 20 |
As can be seen from Table 2, adopting Chinese medicine immune formulation sugar of the present invention significantly to reduce chicken part organ goes outBlood rate, prevention group and attack the remarkable P < 0.01 of malicious control group data difference.
Serum I BDV-HI antibody test result (log2), the results are shown in Table 3.
Table 3 serum I BDV-HI antibody test result (log2)
| Serum number | Prevention group | Attack malicious control group | Blank group |
| 1 | 6.3 | 5.3 | 3.0 |
| 2 | 6.6 | 5.1 | 3.2 |
| 3 | 6.8 | 4.8 | 2.8 |
| 4 | 5.9 | 5.3 | 3.0 |
| 5 | 6.1 | 4.6 | 3.1 |
As can be seen from Table 3, prevention group serum I BDV-HI antibody titer and the blank group difference utmost pointSignificantly (P < 0.01), significant difference compared with attacking malicious group (P < 0.05), illustrates that this Chinese medicine immune formulation can be significantlyImpel the rising of chicken serum IBDV antibody titer.
The impact of SPL conversion ratio.
The SPL conversion ratio of prevention group is 26%, and the SPL conversion ratio of attacking malicious control group is14%, both significant differences (P < 0.01).
Can find out from experiment above, adopt immune formulation of the present invention can significantly reduce Chickens InfectedThe death rate after IBDV, alleviates clinical symptoms, improves serum antibody titer and SPL is turnedRate raises.
All above-mentioned these intellectual properties of primary enforcement, do not set the other forms of enforcement of restriction thisPlant new product and/or new method. Those skilled in the art will utilize this important information, foregoing amendment,To realize similar implementation status. But all modifications or transformation belong to reservation based on new product of the present inventionRight.
The above, be only preferred embodiment of the present invention, is not that the present invention is made to other formRestriction, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changedOr be modified as the equivalent embodiment of equivalent variations. But every technical solution of the present invention content that do not depart from, complies withAny simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention,Still belong to the protection domain of technical solution of the present invention.
Claims (6)
1. for preventing a Chinese medicine immune formulation for infections chicken cloacal bursa, it is characterized in that: bulk drugBy the Radix Astragali, the Chinese bulbul, hairyvein agrimony, eclipta, barrenwort, aleppo avens, the coptis, Samson St Johswort, NanshaGinseng, Chinese gall, Kaempferia galanga, wilsonii, garlic, nutmeg, radish seed, Java brucea, the wind-weed, glossy privetSon, hawthorn, Rose Mallow Root, sealwort, sensitive plant, Radix Codonopsis, kamuning, the fruit of Chinese wolfberry, perilla seed and fish raw meatGrass forms;
In described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 75 weight portion～85 weightPart, the Chinese bulbul 35 weight portion～45 weight portions, hairyvein agrimony 25 weight portion～35 weight portions, eclipta40 weight portion～50 weight portions, barrenwort 25 weight portion～35 weight portions, aleppo avens 45 weight portions～55 weight portions, the coptis 55 weight portion～65 weight portions, Samson St Johswort 30 weight portion～40 weight portions, southThe root of straight ladybell 45 weight portion～55 weight portions, Chinese gall 40 weight portion～50 weight portions, Kaempferia galanga 25 weight portions～35 weight portions, wilsonii 30 weight portion～40 weight portions, garlic 15 weight portion～25 weight portions, meatCardamom 30 weight portion～40 weight portions, radish seed 20 weight portion～30 weight portions, Java brucea 25 weightPart～35 weight portions, the wind-weed 40 weight portion～50 weight portions, the fruit of glossy privet 45 weight portion～55 weight portions,Hawthorn 30 weight portion～40 weight portions, Rose Mallow Root 25 weight portion～35 weight portions, sealwort 45 weight portions～55 weight portions, sensitive plant 30 weight portion～40 weight portions, Radix Codonopsis 45 weight portion～55 weight portions, nineIn fragrant 20 weight portion～30 weight portions, the fruit of Chinese wolfberry 35 weight portion～45 weight portions, perilla seed 20 weightPart～30 weight portions and cordate houttuynia 25 weight portion～35 weight portions.
2. Chinese medicine immune formulation as claimed in claim 1, is characterized in that: described Chinese medicine immune formulationIn the weight portion of each bulk drug be respectively the Radix Astragali 78 weight portion～82 weight portions, the Chinese bulbul 38 weight portions～42 weight portions, hairyvein agrimony 28 weight portion～32 weight portions, eclipta 43 weight portion～47 weight portions,Barrenwort 28 weight portion～32 weight portions, aleppo avens 48 weight portion～52 weight portions, the coptis 58 weightPart～62 weight portions, Samson St Johswort 33 weight portion～37 weight portions, adenophora tetraphylla 48 weight portion～52 weightPart, Chinese gall 43 weight portion～47 weight portions, Kaempferia galanga 28 weight portion～32 weight portions, wilsonii 33Weight portion～37 weight portion, garlic 18 weight portion～22 weight portions, nutmeg 33 weight portion～37 weightsAmount part, radish seed 23 weight portion～27 weight portions, Java brucea 28 weight portion～32 weight portions, the wind-weed43 weight portion～47 weight portions, the fruit of glossy privet 48 weight portion～52 weight portions, hawthorn 33 weight portion～37Weight portion, Rose Mallow Root 28 weight portion～32 weight portions, sealwort 48 weight portion～52 weight portions, sensitive plant33 weight portion～37 weight portions, Radix Codonopsis 48 weight portion～52 weight portions, kamuning 23 weight portion～27Weight portion, the fruit of Chinese wolfberry 38 weight portion～42 weight portions, perilla seed 23 weight portion～27 weight portions and fish raw meatGrass 28 weight portion～32 weight portions.
3. Chinese medicine immune formulation as claimed in claim 1 or 2, is characterized in that: described Chinese medicine immunityIn preparation, the weight portion of each bulk drug is respectively the Radix Astragali 80 weight portions, the Chinese bulbul 40 weight portions, hairyvein agrimony30 weight portions, eclipta 45 weight portions, barrenwort 30 weight portions, aleppo avens 50 weight portions, the coptis60 weight portions, Samson St Johswort 35 weight portions, adenophora tetraphylla 50 weight portions, Chinese gall 45 weight portions, Kaempferia galanga30 weight portions, wilsonii 35 weight portions, garlic 20 weight portions, nutmeg 35 weight portions, radish seed25 weight portions, Java brucea 30 weight portions, the wind-weed 45 weight portions, the fruit of glossy privet 50 weight portions, hawthorn 35Weight portion, Rose Mallow Root 30 weight portions, sealwort 50 weight portions, sensitive plant 35 weight portions, Radix Codonopsis 50 weightsAmount part, kamuning 25 weight portions, the fruit of Chinese wolfberry 40 weight portions, perilla seed 25 weight portions and cordate houttuynia 30Weight portion.
4. the preparation method of Chinese medicine immune formulation described in claim 1 or 2, is characterized in that, comprising:
The first step, by the Radix Astragali, eclipta, barrenwort, aleppo avens, adenophora tetraphylla, Chinese gall, wilsonii,The fruit of glossy privet, sealwort, Radix Codonopsis and the fruit of Chinese wolfberry mix in proportion, get mixture 100g and pack in percolator, addEnter the white wine 600g～800g of 50 DEG C～55 DEG C, under the condition of 25 DEG C～35 DEG C, soak 20 hours～30 littleTime, regulate percolator valve, white wine liquid is slowly oozed, the speed of oozing is 2～4mL per minute,Collect drip, obtain the percolate of 700mL～1L, concentrated, remove white wine, dry, pulverize, obtainThe first dried powder;
Second step, mixes remaining component in proportion, gets 100g mixture, adds 500g～800g'sWater, boils 3～4 hours, filters, and obtains the first filtrate, and filter residue adds the water of 200g～300g again,Again boil 1～2 hour, filter, obtain the second filtrate, the first filtrate and the second filtrate are mixed, denseContracting, except anhydrating, dry, pulverize, and obtains the second dried powder;
The 3rd step, mixes the first dried powder and the second dried powder, obtains Chinese medicine immune formulation.
5. a chicken feed, is characterized in that: raw material by corn, dregs of beans, salt, fish meal, bone meal,Trace mineral supplement, amino acid additive, vitamin(e) supplement and for preventing infections chicken cloacal bursaChinese medicine immune formulation form;
The bulk drug of described Chinese medicine immune formulation by the Radix Astragali, the Chinese bulbul, hairyvein agrimony, eclipta, barrenwort,Aleppo avens, the coptis, Samson St Johswort, adenophora tetraphylla, Chinese gall, Kaempferia galanga, wilsonii, garlic, nutmeg,Radish seed, Java brucea, the wind-weed, the fruit of glossy privet, hawthorn, Rose Mallow Root, sealwort, sensitive plant, Radix Codonopsis, nineIn perfume, the fruit of Chinese wolfberry, perilla seed and cordate houttuynia form;
In described Chinese medicine immune formulation, the weight portion of each bulk drug is respectively the Radix Astragali 75 weight portion～85 weightPart, the Chinese bulbul 35 weight portion～45 weight portions, hairyvein agrimony 25 weight portion～35 weight portions, eclipta40 weight portion～50 weight portions, barrenwort 25 weight portion～35 weight portions, aleppo avens 45 weight portions～55 weight portions, the coptis 55 weight portion～65 weight portions, Samson St Johswort 30 weight portion～40 weight portions, southThe root of straight ladybell 45 weight portion～55 weight portions, Chinese gall 40 weight portion～50 weight portions, Kaempferia galanga 25 weight portions～35 weight portions, wilsonii 30 weight portion～40 weight portions, garlic 15 weight portion～25 weight portions, meatCardamom 30 weight portion～40 weight portions, radish seed 20 weight portion～30 weight portions, Java brucea 25 weightPart～35 weight portions, the wind-weed 40 weight portion～50 weight portions, the fruit of glossy privet 45 weight portion～55 weight portions,Hawthorn 30 weight portion～40 weight portions, Rose Mallow Root 25 weight portion～35 weight portions, sealwort 45 weight portions～55 weight portions, sensitive plant 30 weight portion～40 weight portions, Radix Codonopsis 45 weight portion～55 weight portions, nineIn fragrant 20 weight portion～30 weight portions, the fruit of Chinese wolfberry 35 weight portion～45 weight portions, perilla seed 20 weightPart～30 weight portions and cordate houttuynia 25 weight portion～35 weight portions;
In described feed, the weight portion of each material composition be respectively corn 90 weight portion～110 weight portions,Dregs of beans 30 weight portion～50 weight portions, salt 2 weight portion～4 weight portions, fish meal 8 weight portion～12Weight portion, bone meal 3 weight portion～5 weight portions, trace mineral supplement 2 weight portion～4 weight portions, ammoniaBase acid additive 2 weight portion～4 weight portions, vitamin(e) supplement 2 weight portion～4 weight portions and Chinese medicine are exempted fromEpidemic disease preparation 15 weight portion～20 weight portions.
6. the preparation method of feed described in claim 5, is characterized in that:
The first step, prepares described Chinese medicine immune formulation according to method described in claim 4;
Second step, by the described Chinese medicine immune formulation of other raw material components in feedstuff and first step acquisitionPut into together fodder mixing machine, continuous uniform stirred after 1～3 hour, and put into pulverizer and pulverize,Obtain feed.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101332256A (en) * | 2007-06-28 | 2008-12-31 | 保定冀中药业有限公司 | Traditional Chinese medicine composition for infectious bursal disease and preparation method thereof |
| CN101406637A (en) * | 2008-11-18 | 2009-04-15 | 卢海军 | Medicament for treating infectious bursal disease of chicken |
| CN101698027A (en) * | 2009-10-30 | 2010-04-28 | 重庆市畜牧科学院 | Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof |
| CN102488847A (en) * | 2011-12-20 | 2012-06-13 | 青岛农业大学 | Traditional Chinese medicine composition for treating chicken infectious bursal disease as well as preparation method and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101332256A (en) * | 2007-06-28 | 2008-12-31 | 保定冀中药业有限公司 | Traditional Chinese medicine composition for infectious bursal disease and preparation method thereof |
| CN101406637A (en) * | 2008-11-18 | 2009-04-15 | 卢海军 | Medicament for treating infectious bursal disease of chicken |
| CN101698027A (en) * | 2009-10-30 | 2010-04-28 | 重庆市畜牧科学院 | Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof |
| CN102488847A (en) * | 2011-12-20 | 2012-06-13 | 青岛农业大学 | Traditional Chinese medicine composition for treating chicken infectious bursal disease as well as preparation method and application thereof |
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