CN103592441A - 一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条及其制备方法 - Google Patents
一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条及其制备方法 Download PDFInfo
- Publication number
- CN103592441A CN103592441A CN201310526205.9A CN201310526205A CN103592441A CN 103592441 A CN103592441 A CN 103592441A CN 201310526205 A CN201310526205 A CN 201310526205A CN 103592441 A CN103592441 A CN 103592441A
- Authority
- CN
- China
- Prior art keywords
- colloidal gold
- recombinant swine
- interferon alpha
- swine interferon
- recombinant porcine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 238000012360 testing method Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 102000006992 Interferon-alpha Human genes 0.000 title abstract description 10
- 108010047761 Interferon-alpha Proteins 0.000 title abstract description 10
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 20
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 20
- 241000282898 Sus scrofa Species 0.000 claims description 48
- 102000014150 Interferons Human genes 0.000 claims description 47
- 108010050904 Interferons Proteins 0.000 claims description 47
- 229940079322 interferon Drugs 0.000 claims description 47
- 239000000243 solution Substances 0.000 claims description 19
- 239000010931 gold Substances 0.000 claims description 15
- 229910052737 gold Inorganic materials 0.000 claims description 15
- 238000001556 precipitation Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 239000000084 colloidal system Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 8
- 239000001509 sodium citrate Substances 0.000 claims description 8
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 8
- 229940038773 trisodium citrate Drugs 0.000 claims description 8
- 239000002250 absorbent Substances 0.000 claims description 6
- 230000002745 absorbent Effects 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 229920001223 polyethylene glycol Polymers 0.000 claims description 6
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 238000005571 anion exchange chromatography Methods 0.000 claims description 4
- 206010003445 Ascites Diseases 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 3
- 229920002538 Polyethylene Glycol 20000 Polymers 0.000 claims description 3
- 238000010266 Sephadex chromatography Methods 0.000 claims description 3
- 239000005030 aluminium foil Substances 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000000703 high-speed centrifugation Methods 0.000 claims description 3
- 210000004408 hybridoma Anatomy 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000001742 protein purification Methods 0.000 claims description 3
- 238000003908 quality control method Methods 0.000 claims description 3
- 230000003252 repetitive effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 210000001364 upper extremity Anatomy 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract 4
- 229920002678 cellulose Polymers 0.000 abstract 2
- 239000001913 cellulose Substances 0.000 abstract 2
- 239000011521 glass Substances 0.000 abstract 2
- 229920000915 polyvinyl chloride Polymers 0.000 abstract 2
- 239000004800 polyvinyl chloride Substances 0.000 abstract 2
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract 1
- 238000010200 validation analysis Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 13
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000009182 swimming Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
- G01N33/6866—Interferon
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/52—Assays involving cytokines
- G01N2333/555—Interferons [IFN]
- G01N2333/56—IFN-alpha
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310526205.9A CN103592441B (zh) | 2013-10-29 | 2013-10-29 | 一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310526205.9A CN103592441B (zh) | 2013-10-29 | 2013-10-29 | 一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103592441A true CN103592441A (zh) | 2014-02-19 |
CN103592441B CN103592441B (zh) | 2015-06-17 |
Family
ID=50082663
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310526205.9A Active CN103592441B (zh) | 2013-10-29 | 2013-10-29 | 一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103592441B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107015006A (zh) * | 2017-03-30 | 2017-08-04 | 北京理工大学 | 一种细胞因子免疫层析试纸及其制备方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59122446A (ja) * | 1982-12-28 | 1984-07-14 | Otsuka Pharmaceut Co Ltd | γ−インタ−フエロン関連ペプチド |
WO2002087336A1 (en) * | 2001-04-26 | 2002-11-07 | The United States Of America, As Represented By The Secretary Of Agriculture | Foot and mouth disease virus vaccine |
CN1492930A (zh) * | 2001-02-22 | 2004-04-28 | �����ɷ� | 抗-干扰素α的抗体 |
CN101736062A (zh) * | 2009-11-27 | 2010-06-16 | 王明丽 | 一种重组猪α干扰素标准品的制备方法 |
CN101846675A (zh) * | 2010-03-26 | 2010-09-29 | 赵俊 | 脑膜炎奈瑟菌全菌抗原包被酶标反应板的制法及elisa检测试剂盒 |
CN102841208A (zh) * | 2011-06-24 | 2012-12-26 | 北京乐普医疗科技有限责任公司 | 快速检测肌钙蛋白i的胶体金试纸及其制备方法 |
CN103333251A (zh) * | 2013-04-11 | 2013-10-02 | 广西壮族自治区动物疫病预防控制中心 | 基于重组融合抗原蛋白的γ-干扰素夹心ELISA检测方法 |
-
2013
- 2013-10-29 CN CN201310526205.9A patent/CN103592441B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59122446A (ja) * | 1982-12-28 | 1984-07-14 | Otsuka Pharmaceut Co Ltd | γ−インタ−フエロン関連ペプチド |
CN1492930A (zh) * | 2001-02-22 | 2004-04-28 | �����ɷ� | 抗-干扰素α的抗体 |
WO2002087336A1 (en) * | 2001-04-26 | 2002-11-07 | The United States Of America, As Represented By The Secretary Of Agriculture | Foot and mouth disease virus vaccine |
CN101736062A (zh) * | 2009-11-27 | 2010-06-16 | 王明丽 | 一种重组猪α干扰素标准品的制备方法 |
CN101846675A (zh) * | 2010-03-26 | 2010-09-29 | 赵俊 | 脑膜炎奈瑟菌全菌抗原包被酶标反应板的制法及elisa检测试剂盒 |
CN102841208A (zh) * | 2011-06-24 | 2012-12-26 | 北京乐普医疗科技有限责任公司 | 快速检测肌钙蛋白i的胶体金试纸及其制备方法 |
CN103333251A (zh) * | 2013-04-11 | 2013-10-02 | 广西壮族自治区动物疫病预防控制中心 | 基于重组融合抗原蛋白的γ-干扰素夹心ELISA检测方法 |
Non-Patent Citations (1)
Title |
---|
崔宇超: "现代生物技术在猪病诊断和防治中的应用", 《猪业科学》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107015006A (zh) * | 2017-03-30 | 2017-08-04 | 北京理工大学 | 一种细胞因子免疫层析试纸及其制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN103592441B (zh) | 2015-06-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111089962A (zh) | 联合检测新型冠状病毒IgM/IgG抗体的胶体金试剂盒及制备方法 | |
CN202794178U (zh) | 降钙素原快速定量免疫层析检测试剂盒 | |
CN201886025U (zh) | 一种快速定量检测降钙素原免疫层析试纸条 | |
CN111308072A (zh) | 快速检测新型冠状病毒IgG抗体的胶体金免疫层析试剂盒及其制备方法 | |
CN102854324B (zh) | 快速无损伤性检测肝肿瘤标志物的方法以及采用的试纸条 | |
CN103869079A (zh) | 一种快速定量检测半乳糖凝集素-3的胶体金试纸 | |
CN105510587A (zh) | 新霉素的免疫胶体金检测卡及其制备方法 | |
CN105859843A (zh) | 呼吸道合胞病毒抗原制备方法及利用该抗原制备的呼吸道合胞病毒抗体的快速检测试剂盒 | |
CN100404553C (zh) | 鲟科鱼类卵黄磷蛋白抗体的制备方法及其应用 | |
CN101169414A (zh) | 水体中微囊藻毒素的检测方法 | |
JP2016520846A5 (zh) | ||
CN105486854A (zh) | 一种信号放大方法及相关装置 | |
CN114280296A (zh) | 一种新型冠状病毒(2019-nCOV)IgG/IgM抗体检测试剂盒及其制备方法 | |
CN103739675B (zh) | 草莓镶脉病毒抗体及其抗原多肽、免疫原和应用 | |
CN104407131B (zh) | 一种免疫磁珠底物显色试纸及其制备方法 | |
CN103592441B (zh) | 一种重组猪干扰素ɑ双抗体夹心法免疫胶体金检测试纸条的制备方法 | |
CN103389384B (zh) | Miltenberger抗体检测试纸条及其检测方法 | |
CN105181964A (zh) | 水貂阿留申病毒抗体胶体金检测试纸条及其制备方法 | |
CN105223354A (zh) | 水貂阿留申病毒胶体金免疫层析检测试纸条及其制备方法 | |
CN105486871B (zh) | 一种快速检测犬细小病毒抗体血凝抑制效价的胶体金试纸条、试剂盒及检测方法 | |
CN103823061A (zh) | Au/Fe3O4免疫层析试纸条及其制备方法与应用 | |
CN103267842A (zh) | 检测中成药违法添加双氯芬酸的免疫胶体金方法 | |
CN104345151B (zh) | 提高酶联免疫斑点技术的特异性膜封闭方法 | |
CN104297488A (zh) | 一种抗CBir1抗体检测试纸的制备方法及用途 | |
CN101482565A (zh) | 三聚氰胺胶体金免疫层析检测试纸及制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20160413 Address after: 241009 Anhui province Wuhu Branch Center D Park Economic and Technological Development Zone No. 102, 101 Patentee after: Anhui JiuChuan Biotechnology Co., Ltd. Address before: 230032 Medical University Of Anhui, Hefei, Anhui No. 81 Patentee before: Wang Mingli Patentee before: Zhao Jun |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20160616 Address after: 241009 Anhui province Wuhu Branch Center D Park Economic and Technological Development Zone No. 102, 101 Patentee after: Anhui JiuChuan Biotechnology Co., Ltd. Patentee after: Wuhu Phil Biological Products Industry Research Institute Co. Ltd. Address before: 241009 Anhui province Wuhu Branch Center D Park Economic and Technological Development Zone No. 102, 101 Patentee before: Anhui JiuChuan Biotechnology Co., Ltd. |