CN103499687A - Colloidal gold immunochromatographic assay quantitative detector by combination of internet-of-things data transmission system - Google Patents
Colloidal gold immunochromatographic assay quantitative detector by combination of internet-of-things data transmission system Download PDFInfo
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 230000005540 biological transmission Effects 0.000 title claims abstract description 43
- 238000003556 assay Methods 0.000 title abstract 4
- 238000012360 testing method Methods 0.000 claims description 34
- 238000003317 immunochromatography Methods 0.000 claims description 24
- 238000004891 communication Methods 0.000 claims description 12
- 239000010931 gold Substances 0.000 claims description 7
- 229910052737 gold Inorganic materials 0.000 claims description 7
- 239000000427 antigen Substances 0.000 claims description 5
- 102000036639 antigens Human genes 0.000 claims description 5
- 108091007433 antigens Proteins 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 229920002301 cellulose acetate Polymers 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 40
- 238000000034 method Methods 0.000 abstract description 10
- 238000004458 analytical method Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000013523 data management Methods 0.000 abstract description 2
- 238000013096 assay test Methods 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 description 8
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- 239000012488 sample solution Substances 0.000 description 2
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 238000004817 gas chromatography Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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Abstract
Description
技术领域 technical field
本发明涉及免疫检测装置，具体是一种胶体金免疫层析定量检测仪。 The invention relates to an immunodetection device, in particular to a colloidal gold immunochromatography quantitative detector.
背景技术 Background technique
免疫层析(immunochromatography)技术是是20世纪80年代发展起来的一种将免疫技术和色谱层析技术相结合的快速免疫分析方法。近几年，在国内外兴起已广泛应用于生物和医学检测领域。其原理是以条状纤维层析材料（如，硝酸纤维素膜）为固相，将特异的抗体（或抗原）先固定于条状纤维层析材料的某一区带，当该干燥的硝酸纤维素一端浸入样品液（尿液或血清）后，借助毛细作用使样品溶液在层析条上泳动，同时，使样品中的待测物与层析材料上针对待测物的受体 (如抗体或抗原)发生高特异、高亲和性的免疫反应，层析过程中免疫复合物被富集或截留在层析材料的一定区域 (检测带)，若用免疫胶体金或荧光染料可使该区域显示一定的颜色或荧光条带，短时间 (5～10 min内)便可得到直观的实验结果，从而实现特异性的免疫诊断。它不需进行结合标记物与游离标记物的分离，省去了繁琐的加样、洗涤步骤。因而这种分析技术操作简单快速，分析结果清楚，易于判断，且无须仪器 (或只需简单仪器)，非常适用于食品的现场快速检测。胶体金免疫层析技术是免疫层析技术中应用最为广泛和最为成功的一种，其以胶体金为标记物。 Immunochromatography (immunochromatography) technology is a rapid immunoassay method developed in the 1980s that combines immune technology and chromatography technology. In recent years, it has been widely used in the field of biological and medical detection at home and abroad. The principle is to use the strip-shaped fiber chromatography material (such as nitrocellulose membrane) as the solid phase, and fix the specific antibody (or antigen) on a certain zone of the strip-shaped fiber chromatography material, when the dry nitric acid After immersing one end of the cellulose in the sample solution (urine or serum), the sample solution swims on the chromatographic strip by means of capillary action, and at the same time, the analyte in the sample and the receptor for the analyte on the chromatographic material ( Such as antibodies or antigens) have a highly specific and high affinity immune reaction, and the immune complex is enriched or trapped in a certain area (detection zone) of the chromatographic material during the chromatography process. If immune colloidal gold or fluorescent dyes can be used Make this area display a certain color or fluorescent band, and you can get intuitive experimental results in a short time (within 5-10 minutes), so as to achieve specific immunodiagnosis. It does not require the separation of bound markers and free markers, and saves tedious steps of adding samples and washing. Therefore, this analysis technique is simple and quick to operate, the analysis result is clear, easy to judge, and does not require instruments (or only requires simple instruments), and is very suitable for on-site rapid detection of food. Colloidal gold immunochromatography is the most widely used and successful one in immunochromatography, which uses colloidal gold as a marker.
胶体金是由氯金酸在还原剂如白磷、柠檬酸三钠等的作用下，聚合成特定大小的金颗粒，并由于静电作用成为一种稳定胶体溶液。胶体金颗粒表面负电荷与抗体的正电荷基团会因静电吸附而形成牢固结合，由于胶体金颗粒具有高电子密度的特性，因此金标抗体在相应的配体处大量聚集时，可见黑褐色颗粒或肉眼可见红色或粉红色斑点，进而可以作为免疫层析试验的指示物。现有胶体金免疫层析技术所检测的结果只能给我们一个定性的结果，也就是阳性或者阴性，或者一个非直观的相对值，也就是胶体金颜色的深浅。然而，在食品安全领域中，对于众多的待检物，如金黄色葡萄球菌等，不能只通过其定性筛查结果而判断其是否违规，而是要求报告出待检物的实际含量，特别是一些检测结果阳性的高低意义相差甚远的待检物（如人绒毛膜促性腺激素HCG，甲胎蛋白AFP等）。因此， 实现定量检测的意义重大，目前市场上也有能够完成定量检测的方法，如气相色谱、液相色谱等，但是此类方法需要借助于相应的检测仪器，而且检测时间长，虽然全自动的检测仪器在大批量的检验样本面前能够实现快速检测，但是其主要的缺点有费用昂贵，样本前处理复杂，需要专业人员操作，仪器笨重，不适合现场和基层使用。 Colloidal gold is polymerized into gold particles of a specific size by chloroauric acid under the action of reducing agents such as white phosphorus and trisodium citrate, and becomes a stable colloidal solution due to electrostatic interaction. The negative charges on the surface of the colloidal gold particles and the positively charged groups of the antibody will form a strong combination due to electrostatic adsorption. Because the colloidal gold particles have the characteristics of high electron density, when the gold-labeled antibodies accumulate in large quantities at the corresponding ligands, they can be seen as dark brown. Granules or red or pink spots visible to the naked eye, which in turn can be used as indicators for immunochromatographic tests. The results detected by the existing colloidal gold immunochromatography technology can only give us a qualitative result, that is, positive or negative, or a non-intuitive relative value, that is, the depth of the color of colloidal gold. However, in the field of food safety, for many substances to be inspected, such as Staphylococcus aureus, etc., it is not possible to judge whether they violate the regulations only through the qualitative screening results, but the actual content of the substances to be inspected is required to be reported, especially Some substances to be tested (such as human chorionic gonadotropin HCG, alpha-fetoprotein AFP, etc.) have very different meanings of positive test results. Therefore, it is of great significance to realize quantitative detection. At present, there are methods that can complete quantitative detection on the market, such as gas chromatography, liquid chromatography, etc., but such methods need to rely on corresponding detection instruments, and the detection time is long. Although fully automatic The detection instrument can realize rapid detection in the face of a large number of test samples, but its main disadvantages are expensive, complicated sample pretreatment, professional operation is required, and the instrument is heavy, which is not suitable for on-site and grassroots use.
发明内容 Contents of the invention
针对现有技术的缺陷，本发明的目的是提供一种结合物联网数据传输系统的胶体金免疫层析定量检测仪，该检测仪具有操作简单、便携、价廉，结合胶体金免疫层析试纸条能快速准确的对待测物进行定量检测。通过将检测仪与物联网数据传输系统有机地结合在一起，可以实现对检测数据的更加系统和全面的智能化管理，智能化管理，更加方便政府部门对基层单位的监督和管理。 For the defects of the prior art, the purpose of the present invention is to provide a colloidal gold immunochromatography quantitative detector combined with the Internet of Things data transmission system. The paper strip can quickly and accurately perform quantitative detection of the analyte. By organically combining the detector with the Internet of Things data transmission system, a more systematic and comprehensive intelligent management of the detection data can be realized, and intelligent management is more convenient for government departments to supervise and manage grassroots units.
本发明一种结合物联网数据传输系统的胶体金免疫层析定量检测仪技术方案如下： The technical scheme of a colloidal gold immune chromatography quantitative detector combined with the Internet of Things data transmission system of the present invention is as follows:
包括胶体金免疫层析定量读取仪、胶体金免疫层析试纸条、物联网数据传输系统； Including colloidal gold immunochromatography quantitative reader, colloidal gold immunochromatography test strips, Internet of Things data transmission system;
所述胶体金免疫层析试纸条置于胶体金免疫层析定量读取仪中用于上样；胶体金免疫层析试纸条包括在底板上依次搭连地粘贴滤纸、样本垫、金标垫、NC膜和吸水纸，粘贴好的试纸条板用切割机裁切成试纸条；醋酸纤维素膜喷涂有待检物抗原或抗体及二抗喷涂，分别作为检测线T线与质控线C线；金标垫上喷涂有金标抗体； The colloidal gold immunochromatography test strip is placed in the colloidal gold immunochromatography quantitative reader for sample loading; the colloidal gold immunochromatography test strip includes successively pasting filter paper, sample pad, gold Standard pad, NC film and absorbent paper, and the pasted test strip strips are cut into test strips with a cutting machine; the cellulose acetate membrane is sprayed with the antigen or antibody of the object to be tested and the secondary antibody is sprayed, which are used as the test line T line and quality test line respectively. Control line C line; the gold label pad is sprayed with gold label antibody;
所述的物联网数据传输系统包括包括微控制器及用于接收传送数据的GPRS/GSM模块。微控制器与GPRS/GSM模块通过串口相连接进行通信。微控制器接收用户数据，将数据按相关AT指令格式传送给GPRS/GSM模块，GPRS/GSM模块再将数据发送给数据中心，进而实现用户与数据中心的通信连接。 The IoT data transmission system includes a microcontroller and a GPRS/GSM module for receiving and transmitting data. The microcontroller communicates with the GPRS/GSM module through a serial port connection. The microcontroller receives user data, and transmits the data to the GPRS/GSM module according to the relevant AT command format, and the GPRS/GSM module then sends the data to the data center, thereby realizing the communication connection between the user and the data center.
微控制器可以选用的是意法半导体公司生产的基于ARM Cortex-M3内核的32位单片机STM32F101RBT6。其主频可高达36 MHZ，128 kB FLASH 以及16 kB SRAM。GPRS/GSM 模块可以采用中兴通讯公司生产的MG2639模块，其可同时连接6路IP或UDP链路，并且单次接收最高4.5 k的用户数据并发送，以及接收最高1.3 k的服务器数据。 The microcontroller can be selected from the 32-bit microcontroller STM32F101RBT6 based on the ARM Cortex-M3 core produced by STMicroelectronics. Its main frequency can be up to 36 MHZ, 128 kB FLASH and 16 kB SRAM. The GPRS/GSM module can use the MG2639 module produced by ZTE Corporation, which can connect 6 IP or UDP links at the same time, and receive and send up to 4.5 k user data at a time, and receive up to 1.3 k server data.
所述的物联网数据传输单元与手持设备的通信方式是通过232串口与本地手持检测设备进行通信；后台数据中心通过GPRS网络与物联网数据传输系统进行连接，从而实现数据中心与手持检测设备的通信。数据中心将菜单数据通过物联网数据传输系统传输给手持检测设备，手持检测设备通过液晶屏将菜单数据显示出。手持检测设备获取检测数据后将其发回物联网数据传输系统，物联网数据传输系统即刻将检测数据发往数据中心。上述“菜单数据”及“检测数据”的写入、上传按照图4协议进行封装。 The communication mode of the described Internet of Things data transmission unit and the handheld device is to communicate with the local handheld detection device through the 232 serial port; the background data center is connected with the Internet of Things data transmission system through the GPRS network, thereby realizing the communication between the data center and the handheld detection device communication. The data center transmits the menu data to the handheld detection device through the Internet of Things data transmission system, and the handheld detection device displays the menu data through the LCD screen. The hand-held testing device obtains the testing data and sends it back to the IoT data transmission system, and the IoT data transmission system immediately sends the testing data to the data center. The writing and uploading of the above "menu data" and "detection data" are encapsulated according to the protocol in Figure 4.
物联网数据传输系统的主要组成结构如图2示。软件系统可使用多种工作方式，包括单联透传，主备中心切换及唤醒模式（包括短信唤醒，电话唤醒，用户数据唤醒）。图3为软件程序流程图，其详细说明了整个软件系统的工作的过程。 The main structure of the IoT data transmission system is shown in Figure 2. The software system can use a variety of working methods, including single-link transparent transmission, master and backup center switching, and wake-up modes (including SMS wake-up, phone wake-up, and user data wake-up). FIG. 3 is a flowchart of the software program, which details the working process of the entire software system.
为了使数据传输更稳定可靠，物联网数据传输单元按照图4协议与手持设备进行通信，以时刻提示用户本物联网数据传输单元的链路连接状态。控制码选择 0x22，0x22(链路连接成功)，0x33，0x33(链路连接中)，其他不变。常态下，物联网数据传输单元将每隔一分钟报告链路连接状态，但当链路发生变化时则立刻报告。手持设备只有在确认链路连接成功的状态下方可向数据中心发送检测数据。 In order to make the data transmission more stable and reliable, the IoT data transmission unit communicates with the handheld device according to the protocol in Figure 4 to remind the user of the link connection status of the IoT data transmission unit at all times. The control code selection is 0x22, 0x22 (the link is successfully connected), 0x33, 0x33 (the link is being connected), and the others remain unchanged. Normally, the IoT data transmission unit will report the link connection status every minute, but it will report immediately when the link changes. The handheld device can only send detection data to the data center after confirming that the link connection is successful.
所述胶体金免疫层析定量读取仪可以采用常规的胶体金免疫层析定量读取仪。或者有精密机械运动控制系统、光学系统、电学系统以及计算机系统组成。即其是一种由光、机、电、计算机组成的精密仪器，由特制的小功率高亮度的绿色LED通过一组透镜照射至胶体金免疫层析试纸条，胶体金免疫层析试纸条上胶体金所产生的反射光通过另一组透镜组被光电转换接收器所采集，通过测量试纸条检测带与质控带对绿色波长的吸收光密度判断被检物的浓度。仪器的计算机系统通过对LED的控制、精密机械运动的控制、极弱光信号检测和数据统计分析完成，并且我们运用的数据分析如背景补偿、检测时间的控制可准确地找到检测带与质控带的位置，提高了弱信号试纸条测量重复性，减少人工干预，大大提升了仪器的智能化程度。其具体的读取仪结构及工作原理如图1所示。 The colloidal gold immunochromatographic quantitative reader can be a conventional colloidal gold immunochromatographic quantitative reader. Or it is composed of precision mechanical motion control system, optical system, electrical system and computer system. That is to say, it is a precision instrument composed of light, machine, electricity and computer. A special low-power and high-brightness green LED is irradiated to the colloidal gold immunochromatography test strip through a set of lenses, and the colloidal gold immunochromatography test strip The reflected light generated by the colloidal gold on the strip is collected by the photoelectric conversion receiver through another lens group, and the concentration of the tested substance is judged by measuring the absorption optical density of the test strip and the quality control strip on the green wavelength. The computer system of the instrument is completed through the control of LED, the control of precision mechanical movement, the detection of extremely weak light signals and the statistical analysis of data, and the data analysis we use such as background compensation and control of detection time can accurately find the detection zone and quality control The position of the belt improves the measurement repeatability of weak signal test strips, reduces manual intervention, and greatly improves the intelligence of the instrument. Its specific reader structure and working principle are shown in Figure 1.
采用本发明技术方案具有如下有益效果： Adopting the technical solution of the present invention has the following beneficial effects:
1、本发明借助了胶体金免疫层析技术快速简单的优点，在实际检测中，缩短了分析检测的时间和简化了实验操作，特别适合偏远基层单位和现场使用。 1. The present invention takes advantage of the fast and simple advantages of colloidal gold immunochromatography technology, shortens the analysis and detection time and simplifies the experimental operation in actual detection, and is especially suitable for remote grass-roots units and on-site use.
2、本发明结合了读取仪体积小、重量轻、便于携带，能够独立处理数据，达到定量检测的功能的优点，特别适合现场使用。 2. The present invention combines the advantages of small size, light weight, portability, independent processing of data, and the function of quantitative detection of the reader, and is especially suitable for on-site use.
3、本发明通过物联网数据传输单元与手持设备的通信，同时后台数据中心通过GPRS网络与物联网数据传输单元进行连接，从而实现数据中心与手持检测设备的通信。因此，可以通过随身携带的手机、平板电脑等通信设备就能够实现数据的远程管理，成功代替了以前一些较为笨重的通讯设备，使得在实际应用中更为便携。 3. The present invention communicates with the handheld device through the data transmission unit of the Internet of Things, and at the same time, the background data center is connected with the data transmission unit of the Internet of Things through the GPRS network, thereby realizing the communication between the data center and the handheld detection device. Therefore, the remote management of data can be realized through mobile phones, tablet computers and other communication devices, which have successfully replaced some of the more cumbersome communication devices in the past, making it more portable in practical applications.
4、本发明采用了物联网系统强大的数据管理和传输能力，有效地实现了对检测数据的现场分析以及回顾性分析，便于数据管理中心对检测结果的实时操控。特别方便政府部门对基层单位的监督和管理。尤其是在发生重大食品安全事件时，可以很好的实现食品样品的溯源。 4. The present invention adopts the powerful data management and transmission capabilities of the Internet of Things system, effectively realizes the on-site analysis and retrospective analysis of the detection data, and facilitates the real-time manipulation of the detection results by the data management center. It is especially convenient for government departments to supervise and manage grassroots units. Especially when a major food safety incident occurs, the traceability of food samples can be well realized.
附图说明 Description of drawings
图1 读取仪工作原理图。 Figure 1 Schematic diagram of the working principle of the reader.
图2 物联网数据传输单元硬件系统的主要组成结构图示。 Figure 2 is a schematic diagram of the main components of the hardware system of the IoT data transmission unit.
图3 物联网数据传输单元软件系统操作程序流程图。 Figure 3 Flowchart of the operating program of the software system of the IoT data transmission unit.
图4 “菜单数据”及“检测数据”的写入、上传方法协议。 Figure 4 The protocol for writing and uploading "menu data" and "detection data".
具体实施方式 Detailed ways
为了使本发明更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅用以解释本发明，并不用于限定本发明。 In order to make the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
实施例1 Example 1
本发明一种结合物联网数据传输系统的胶体金免疫层析定量检测仪包括胶体金免疫层析定量读取仪、胶体金免疫层析试纸条、物联网数据传输系统； A colloidal gold immune chromatography quantitative detector combined with an Internet of Things data transmission system of the present invention includes a colloidal gold immune chromatography quantitative reader, a colloidal gold immune chromatography test strip, and an Internet of Things data transmission system;
所述胶体金免疫层析试纸条置于胶体金免疫层析定量读取仪中用于上样；胶体金免疫层析试纸条包括在底板上依次搭连地粘贴滤纸、样本垫、金标垫、NC膜和吸水纸，粘贴好的试纸条板用切割机裁切成试纸条；醋酸纤维素膜喷涂有待检物抗原或抗体及二抗喷涂，分别作为检测线T线与质控线C线；金标垫上喷涂有金标抗体； The colloidal gold immunochromatography test strip is placed in the colloidal gold immunochromatography quantitative reader for sample loading; the colloidal gold immunochromatography test strip includes successively pasting filter paper, sample pad, gold Standard pad, NC film and absorbent paper, and the pasted test strip strips are cut into test strips with a cutting machine; the cellulose acetate membrane is sprayed with the antigen or antibody of the object to be tested and the secondary antibody is sprayed, which are used as the test line T line and quality test line respectively. Control line C line; the gold label pad is sprayed with gold label antibody;
所述胶体金免疫层析定量读取仪可以采用常规的胶体金免疫层析定量读取仪。 The colloidal gold immunochromatographic quantitative reader can be a conventional colloidal gold immunochromatographic quantitative reader.
所述的物联网数据传输系统包括包括微控制器及用于接收传送数据的GPRS/GSM模块。微控制器与GPRS/GSM模块通过串口相连接进行通信。微控制器接收用户数据，将数据按相关AT指令格式传送给GPRS/GSM模块，GPRS/GSM模块再将数据发送给数据中心，进而实现用户与数据中心的通信连接。 The IoT data transmission system includes a microcontroller and a GPRS/GSM module for receiving and transmitting data. The microcontroller communicates with the GPRS/GSM module through a serial port connection. The microcontroller receives user data, and transmits the data to the GPRS/GSM module according to the relevant AT command format, and the GPRS/GSM module then sends the data to the data center, thereby realizing the communication connection between the user and the data center.
微控制器可以选用的是意法半导体公司生产的基于ARM Cortex-M3内核的32位单片机STM32F101RBT6。其主频可高达36 MHZ，128 kB FLASH 以及16 kB SRAM。GPRS/GSM 模块可以采用中兴通讯公司生产的MG2639模块，其可同时连接6路IP或UDP链路，并且单次接收最高4.5 k的用户数据并发送，以及接收最高1.3 k的服务器数据。 The microcontroller can be selected from the 32-bit microcontroller STM32F101RBT6 based on the ARM Cortex-M3 core produced by STMicroelectronics. Its main frequency can be up to 36 MHZ, 128 kB FLASH and 16 kB SRAM. The GPRS/GSM module can use the MG2639 module produced by ZTE Corporation, which can connect 6 IP or UDP links at the same time, and receive and send up to 4.5 k user data at a time, and receive up to 1.3 k server data.
所述的物联网数据传输单元与手持设备的通信方式是通过232串口与本地手持检测设备进行通信；后台数据中心通过GPRS网络与物联网数据传输系统进行连接，从而实现数据中心与手持检测设备的通信。数据中心将菜单数据通过物联网数据传输系统传输给手持检测设备，手持检测设备通过液晶屏将菜单数据显示出。手持检测设备获取检测数据后将其发回物联网数据传输系统，物联网数据传输系统即刻将检测数据发往数据中心。上述“菜单数据”及“检测数据”的写入、上传按照图4协议进行封装。 The communication mode of the described Internet of Things data transmission unit and the handheld device is to communicate with the local handheld detection device through the 232 serial port; the background data center is connected with the Internet of Things data transmission system through the GPRS network, thereby realizing the communication between the data center and the handheld detection device communication. The data center transmits the menu data to the handheld detection device through the Internet of Things data transmission system, and the handheld detection device displays the menu data through the LCD screen. The hand-held testing device obtains the testing data and sends it back to the IoT data transmission system, and the IoT data transmission system immediately sends the testing data to the data center. The writing and uploading of the above "menu data" and "detection data" are encapsulated according to the protocol in Figure 4.
物联网数据传输系统的主要组成结构如图2示。软件系统可使用多种工作方式，包括单联透传，主备中心切换及唤醒模式（包括短信唤醒，电话唤醒，用户数据唤醒）。图3为软件程序流程图，其详细说明了整个软件系统的工作的过程。 The main structure of the IoT data transmission system is shown in Figure 2. The software system can use a variety of working methods, including single-link transparent transmission, master and backup center switching, and wake-up modes (including SMS wake-up, phone wake-up, and user data wake-up). FIG. 3 is a flowchart of the software program, which details the working process of the entire software system.
为了使数据传输更稳定可靠，物联网数据传输单元按照图4协议与手持设备进行通信，以时刻提示用户本物联网数据传输单元的链路连接状态。控制码选择 0x22，0x22(链路连接成功)，0x33，0x33(链路连接中)，其他不变。常态下，物联网数据传输单元将每隔一分钟报告链路连接状态，但当链路发生变化时则立刻报告。手持设备只有在确认链路连接成功的状态下方可向数据中心发送检测数据。 In order to make the data transmission more stable and reliable, the IoT data transmission unit communicates with the handheld device according to the protocol in Figure 4 to remind the user of the link connection status of the IoT data transmission unit at all times. The control code selection is 0x22, 0x22 (the link is successfully connected), 0x33, 0x33 (the link is being connected), and the others remain unchanged. Normally, the IoT data transmission unit will report the link connection status every minute, but it will report immediately when the link changes. The handheld device can only send detection data to the data center after confirming that the link connection is successful.
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