CN219935864U - Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles - Google Patents
Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles Download PDFInfo
- Publication number
- CN219935864U CN219935864U CN202320475073.0U CN202320475073U CN219935864U CN 219935864 U CN219935864 U CN 219935864U CN 202320475073 U CN202320475073 U CN 202320475073U CN 219935864 U CN219935864 U CN 219935864U
- Authority
- CN
- China
- Prior art keywords
- fluorescent
- pad
- membrane
- detection
- quality control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 24
- 239000004793 Polystyrene Substances 0.000 title claims abstract description 23
- 229920002223 polystyrene Polymers 0.000 title claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 19
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 42
- 239000012528 membrane Substances 0.000 claims abstract description 33
- 238000003908 quality control method Methods 0.000 claims abstract description 22
- 238000004458 analytical method Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 239000000020 Nitrocellulose Substances 0.000 claims description 8
- 229920001220 nitrocellulos Polymers 0.000 claims description 8
- 239000003365 glass fiber Substances 0.000 claims description 4
- 239000004677 Nylon Substances 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 229920002301 cellulose acetate Polymers 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 229920006267 polyester film Polymers 0.000 claims description 3
- 239000000126 substance Substances 0.000 description 16
- 238000005516 engineering process Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000007720 emulsion polymerization reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- -1 rare earth ion Chemical class 0.000 description 2
- 229910052761 rare earth metal Inorganic materials 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
The fluorescent immunochromatography reagent strip comprises a bottom plate, wherein a sample pad, a bonding pad, an analysis membrane and a water absorption pad are sequentially arranged on the bottom plate from left to right, detection molecules and one or more quality control molecules of detection indexes marked by the polystyrene fluorescent nanoparticles are coated on the bonding pad, a detection line and a quality control line are arranged on the analysis membrane, a molecule capture molecule to be detected is coated on the detection line, and a quality control molecule capture molecule is coated on the quality control line.
Description
Technical Field
The utility model relates to the technical field of medical inspection, in particular to a fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles.
Background
The immunochromatography technology is a rapid diagnosis technology which is developed in recent years, and has the characteristics of accuracy, rapidness, simplicity in operation and the like. The immunochromatography technology, in particular the colloidal gold immunochromatography technology, is a relatively mature, stable, quick, simple and convenient on-site diagnosis technology, but can only detect one index, is applied to a semi-automatic detection instrument, and is a technology for realizing full-automatic detection of multiple indexes at the same time.
Disclosure of Invention
Aiming at the situation, the utility model aims to overcome the defects of the prior art and provides a fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles, which effectively solves the problem that various indexes cannot be detected fully automatically.
The technical scheme includes that the device comprises a bottom plate, a sample pad, a combination pad, an analysis membrane and a water absorption pad are sequentially arranged on the bottom plate from left to right, detection molecules and one or more quality control molecules of detection indexes marked by polystyrene fluorescent nanoparticles are coated on the combination pad, a detection line and a quality control line are arranged on the analysis membrane, a molecule capture molecule to be detected is coated on the detection line, and a quality control molecule capture molecule is coated on the quality control line.
Preferably, the height of the sample pad is greater than the height of the bonding pad, the height of the bonding pad is greater than the height of the analysis membrane, the height of the analysis membrane is less than the height of the water absorption pad, and the sample pad, the bonding pad, the analysis membrane and the water absorption pad are sequentially and partially overlapped and overlapped on the bottom plate.
Preferably, the number of the detection lines is two.
Preferably, the particle size of the polystyrene nanoparticle is 10-500nm.
Preferably, the bonding pad is a polyester film or a glass fiber film.
Preferably, the analysis membrane is a nitrocellulose membrane, a nylon membrane or a nitrocellulose/cellulose acetate mixed membrane.
The preparation method of the fluorescent immunochromatographic reagent strip based on the polystyrene fluorescent nanoparticles comprises the following steps:
1) Fluorescent substance labeled antibody: synthesizing fluorescent substances, respectively connecting specific antibodies with different detection indexes and quality control molecules with the fluorescent substances to obtain antibody/quality control molecule modified fluorescent substances, and fixing the materials on a binding pad;
2) Antibody coating: the detection line and the quality control line are respectively arranged on the analysis film, capture molecules capable of being specifically combined with the quality control molecules are fixed, and the detection line is used for fixing the specific capture molecules of different antigenic determinants of the molecules to be detected:
3) Assembling the reagent strip: a sample pad, a combination pad, an analysis membrane, a water absorption pad and a bottom plate are used for constructing a fluorescent immunochromatography reagent strip, and a chopper is used for cutting the reagent strip into a certain width to obtain the required reagent strip.
Compared with the traditional equipment, the utility model has the following advantages: 1) The technology for synthesizing the polystyrene fluorescent nanoparticles is combined with the application of the immunochromatography technology, the reagent strip adopts the polystyrene fluorescent nanoparticles technology to label the antibody, and compared with the existing fluorescent substances such as quantum dots, the preparation is simple, the cost is low, and the labeling and detection performances are good; 2) The reagent strip can be applied to various instruments such as full-automatic instruments, semi-automatic instruments and the like, and particularly can be applied to a full-automatic fluorescence immunity quantitative analysis device, so that the detection efficiency is greatly improved.
Drawings
Fig. 1 is a schematic diagram of a front view of the present utility model.
Detailed Description
The following describes the embodiments of the present utility model in further detail with reference to the drawings.
The fluorescent immunochromatographic reagent strip based on the polystyrene fluorescent nanoparticles comprises a bottom plate 1, wherein a sample pad 2, a bonding pad 3, an analysis membrane 4 and a water absorption pad 5 are sequentially arranged on the bottom plate 1 from left to right, detection molecules and one or more quality control molecules of detection indexes marked by the polystyrene fluorescent nanoparticles are coated on the bonding pad 3, a detection line 6 and a quality control line 7 are arranged on the analysis membrane 4, a detection line 6 is coated with a molecule capture molecule to be detected, and a quality control molecule capture molecule is coated on the quality control line 7.
In order to facilitate the reagent flow, the height of the sample pad 2 is greater than that of the binding pad 3, the height of the binding pad 3 is greater than that of the analysis membrane 4, the height of the analysis membrane 4 is less than that of the water absorption pad 5, and the sample pad 2, the binding pad 3, the analysis membrane 4 and the water absorption pad 5 are sequentially and partially overlapped on the bottom plate 1.
The number of the detection lines 6 is two.
The particle size of the polystyrene nano particles is 10-500nm.
The bonding pad 3 is a polyester film or a glass fiber film.
The analysis membrane 4 is a nitrocellulose membrane, a nylon membrane or a nitrocellulose/cellulose acetate mixed membrane.
The bottom plate 1 is a plastic plate.
When the utility model is used, the terms of front, back, left, right, up, down and the like indicate azimuth or position relation are based on a front view, when immunochromatography is started, an index molecule to be detected, a detection molecule and a fluorescent substance are combined on a combining pad 3, then the combined index molecule is captured by a capture molecule in a detection line 6 on an analysis film 4 to form a capture molecule-to-be-detected molecule-detection molecule-fluorescent substance compound, the capture molecule-to-be-detected molecule-fluorescent substance compound is placed under a fluorescent instrument to be detected, excitation light is irradiated on the detection line 6, the fluorescent substance emits emitted light after excitation, a detection value is calculated after the fluorescent substance is captured by a photoelectric material, and detection of a detection index is completed;
the polystyrene nano particles are prepared by using styrene, then adding more than one component of acrylic acid, methacrylic acid, methyl methacrylate or acrylamide, mixing, and performing emulsion polymerization, seed emulsion polymerization or soap-free emulsion polymerization, wherein the inside of the polystyrene fluorescent nano particles is a hydrophobic environment, and the polystyrene nano particles have remarkable fluorescent protection effect on fluorescent substances, particularly rare earth ion chelate with fluorescent light easily quenched by water molecules, so that the fluorescence of fluorescent molecules in the particles is enhanced.
The preparation method of the fluorescent immunochromatography reagent strip comprises the following steps:
1) Polystyrene fluorescent nanoparticles are coupled with different detection molecules: dissolving in 20-100Mol PBS buffer solution, dissolving activated 360nm fluorescent substance in dimethyl sulfoxide (DMF), mixing detection molecule and 360nm fluorescent substance at a ratio of 1:1-1:3, stirring thoroughly for reaction for 1h, placing the solution in dialysis bag, and performing gradient liquid exchange dialysis for 3 times, each for 6-8h to obtain the solution which is the detection molecule-fluorescent substance compound; 2) Preparation of the bonding pad 3: the bonding pad 3 is made of glass fiber or polyester, then the bonding pad 3 is soaked in 50mM PBS (H=7.5) buffer solution, taken out and dried, and the prepared detection molecule-fluorescent substance compound is uniformly sprayed on the bonding pad 3; 3) Preparation of analytical membrane 4: in the method, a detection line 6 and a quality control line 7 are preferably fused into one line, capture molecules of indexes to be detected and quality control molecules are diluted to the concentration of 4mg/ml by using 50mM PBS (phosphate buffer solution with the pH value of 7.5), the detection line 6 is obtained by scribing the right end of a nitrocellulose membrane at the concentration of 0.8 mu L/cm, and a second detection line 6 is scribed at the position, which is 5mM away from the detection line 6; 4) And (3) assembling: the sample pad 2, the bonding pad 3, the nitrocellulose membrane and the absorbent paper are sequentially overlapped on the bottom plate 1, and the test strip with proper width is cut according to the requirement.
The utility model has great market demands, such as ovulation monitoring and glycosylated hemoglobin measurement, and in addition, some items suitable for emergency treatment, such as cardiac marker products, infectious disease series products and drug series products, also need to adopt quantitative, rapid, random and simple-operation immunodetection modes: such assays are also suitable for prospecting, military, food and community and small hospital use, and are commonly referred to as Point Of Care tests.
The low sensitivity and the narrow measurement range are disadvantages which are unavoidable in quantitative/semi-quantitative immunochromatography using nano gold as a label. By using fluorescent nano particles as a mark, the analysis sensitivity and the measurement range of immunochromatography detection are greatly improved by detecting fluorescent signals, particularly long-life fluorescent signals of rare earth ion chelate.
The present embodiment is not limited in any way by the shape, material, structure, etc. of the present utility model, and any simple modification, equivalent variation and modification made to the above embodiments according to the technical substance of the present utility model are all included in the scope of protection of the technical solution of the present utility model.
Claims (5)
1. The fluorescent immunochromatography reagent strip based on the polystyrene fluorescent nanoparticles is characterized by comprising a bottom plate (1), wherein a sample pad (2), a binding pad (3), an analysis membrane (4) and a water absorption pad (5) are sequentially arranged on the bottom plate (1) from left to right, detection molecules and one or more quality control molecules of detection indexes marked by the polystyrene fluorescent nanoparticles are coated on the binding pad (3), a detection line (6) and a quality control line (7) are arranged on the analysis membrane (4), a molecule capture molecule to be detected is coated on the detection line (6), and a quality control molecule capture molecule is coated on the quality control line (7).
2. The fluorescent immunochromatographic reagent strip based on polystyrene fluorescent nanoparticles according to claim 1, wherein the sample pad (2) has a height greater than that of the binding pad (3), the binding pad (3) has a height greater than that of the analytical membrane (4), the analytical membrane (4) has a height smaller than that of the absorbent pad (5), and the sample pad (2), the binding pad (3), the analytical membrane (4) and the absorbent pad (5) are sequentially overlapped on the base plate (1) in a partial overlap manner.
3. The fluorescent immunochromatographic reagent strip based on polystyrene fluorescent nanoparticles according to claim 1, wherein the number of detection lines (6) is two.
4. The fluorescent immunochromatographic reagent strip based on polystyrene fluorescent nanoparticles according to claim 1, wherein the binding pad (3) is a polyester film or a glass fiber film.
5. The fluorescent immunochromatographic strip based on polystyrene fluorescent nanoparticles according to claim 1, in which the analytical membrane (4) is a nitrocellulose membrane, a nylon membrane or a nitrocellulose/cellulose acetate mixed membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320475073.0U CN219935864U (en) | 2023-03-14 | 2023-03-14 | Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320475073.0U CN219935864U (en) | 2023-03-14 | 2023-03-14 | Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219935864U true CN219935864U (en) | 2023-10-31 |
Family
ID=88489901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320475073.0U Active CN219935864U (en) | 2023-03-14 | 2023-03-14 | Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219935864U (en) |
-
2023
- 2023-03-14 CN CN202320475073.0U patent/CN219935864U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4832396B2 (en) | Test apparatus, holder for test strip, kit for detecting the presence of an analyte in a sample, and method for detecting an analyte in a sample | |
| US11371987B2 (en) | Method of amplifying detection light using light-reflecting material, in immunochromatography | |
| KR20140143120A (en) | Assay strip having variable control line, and diagnosis kit using the same | |
| TW200413726A (en) | Self-calibrated flow-through assay devices | |
| US20060105402A1 (en) | Blood type method system and device | |
| JP2011117906A (en) | Immunochromatography method | |
| CN107328931A (en) | A kind of quick continuous detection technique based on nano-probe and magnetic micro-nano granules | |
| CN101120253A (en) | Immunochromatographic test instrument and semiquantitative method using the same | |
| CN103645314A (en) | Test strip card capable of automatically filtering sample | |
| CA2532023A1 (en) | Particle agglutination detection method and device | |
| CN113156105A (en) | A type botulinum toxin rapid quantitative detection card | |
| CN219935864U (en) | Fluorescent immunochromatography reagent strip based on polystyrene fluorescent nanoparticles | |
| JP2010071828A (en) | Method for detecting substance to be examined | |
| CN106526166A (en) | Rapid detection of lean meat powder in pork | |
| JP2009192222A (en) | Immunoassay method | |
| JP4229943B2 (en) | Specimen inspection method and specimen storage container used in the inspection method | |
| CN106645703A (en) | Kit and method for quickly and quantitatively detecting small-molecular compounds | |
| CN114544974A (en) | Carbon quantum dot microsphere-based fluorescence immunochromatographic reagent card and preparation method and application thereof | |
| CN210923728U (en) | Time-resolved fluorescence S-100 β protein detection card | |
| CN117169519B (en) | Dissociation agent and kit for detecting TT3 and/or TT4 in sample | |
| CN108535484A (en) | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method quantitatively detecting fPSA in blood | |
| CN205176037U (en) | Can quantitative immunity chromatography device | |
| CN218727337U (en) | Film chromatography detection combined structure capable of performing connection and disconnection conversion between assemblies | |
| CN215415460U (en) | Rapid detection test strip and rapid detection card | |
| CN103529214B (en) | Method for detecting treponema pallidum, quantum-dot labeled immunochromatography test paper and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |