CN207689495U - A kind of whole blood immunity detection device - Google Patents
A kind of whole blood immunity detection device Download PDFInfo
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- CN207689495U CN207689495U CN201721800206.8U CN201721800206U CN207689495U CN 207689495 U CN207689495 U CN 207689495U CN 201721800206 U CN201721800206 U CN 201721800206U CN 207689495 U CN207689495 U CN 207689495U
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Abstract
The utility model discloses a kind of whole blood immunity detection device, including micro-fluidic chip, loading slot, instrument connection and raceway groove are provided on micro-fluidic chip, loading slot is connected to instrument connection by raceway groove, nanogold particle and detection probe are provided in instrument connection, one end of instrument connection is provided with parallel optical assembly, and the other end of instrument connection is provided with spectral analysis module.This programme in loading slot by being arranged hemofiltration film, and nanogold particle and detection probe are set in instrument connection, allow the device that directly whole blood to be added in micro-fluidic chip, realize that whole blood separation obtains serum by hemofiltration film, it is then dispensed for instrument connection, whole process is easy to operate, to user it is professional require it is low.And LSPR effect of the principle of this programme based on nanogold particle, detection process is without adding any marker, simple testing process.
Description
Technical field
The utility model is related to the field of medical instrument technology more particularly to a kind of whole blood immunity detection devices.
Background technology
With the raising of people's living standard and know-how, requirement of the people to quality of the life and health is increasingly
Height, it is desirable to periodically understand and grasp the physical condition of itself, but arrive every time medical institutions check body take it is too long and
Expense is also very high, and feasibility is very low.Therefore, there is an urgent need to the detection devices that some families use by people, with to own bodies shape
Condition is detected and understands.
Currently, there are many immunity testing equipments on the market, these equipment can pass through the blood testing and analysis of human body
The physical condition of human body, but the detection sample object of these equipment is not whole blood, but serum or blood plasma, it also needs to simultaneously
Increase additional markers object or isotopic label, equipment is very complicated, and detecting step is more, and operation difficulty is big, to the professional of user
It is required that very much, the convenience used is very low, cannot be satisfied the requirement of people.
Based on the above situation, it is necessary to design a kind of quickly and easily immunity testing equipment for we.
Utility model content
One purpose of the utility model is：A kind of whole blood immunity detection device is provided, can be made with the whole blood of human body
To detect sample object, the use difficulty of user is effectively reduced, convenience is improved.
Another purpose of the utility model is：A kind of whole blood immunity detection device is provided, based on nanogold particle
LSPR effects are detected, and without adding any marker, simple testing process, the consumptive material for testing needs is less, use cost
It is low.
For this purpose, the utility model uses following technical scheme：
A kind of whole blood immunity detection device, including micro-fluidic chip, which is characterized in that include adding on the micro-fluidic chip
Sample slot, instrument connection and raceway groove, the loading slot are connected to the instrument connection by the raceway groove, and filter is provided in the loading slot
Blood film is provided with nanogold particle and detection probe in the instrument connection, and one end of the instrument connection is provided with parallel optical assembly,
The other end of the instrument connection is provided with spectral analysis module.
Preferably, the loading slot is opened in the central region of the micro-fluidic chip, and the instrument connection is opened in described
The edge of micro-fluidic chip.
Preferably, the micro-fluidic chip is thin rounded flakes.
Preferably, the micro-fluidic chip is lamination layer structure, and the lamination layer structure is divided into main layer and auxiliary layer.It is preferred that
, the micro-fluidic chip main layer is made of hard material, and auxiliary layer is made of elastic material.
Preferably, the raceway groove is formed between main layer and auxiliary layer structure.
Preferably, in the position of the correspondence raceway groove, side of the auxiliary layer far from the raceway groove is provided with valve.Tool
Body, when the valve in the closure state, the valve is not contacted with the auxiliary layer, when the valve is in opening state
When state, the valve oppresses the auxiliary layer, and the auxiliary layer elastic material, which deforms upon, makes auxiliary layer be contacted with main layer, with this
To control the closure of the raceway groove.
Preferably, the valve quantity is more than one, is arranged at equal intervals.Specifically, the valve is arranged on raceway groove,
Valve quantity is adjusted according to the length of raceway groove and the length of spacing distance, and when in raceway groove filled with liquid, Open valve successively can
The liquid in raceway groove to be discharged, achieve the effect that liquid is preferably driven to flow with this.
Preferably, the instrument connection is through hole or blind hole.
Preferably, the shape of the instrument connection is cylindrical.
Preferably, the diameter of the nanogold particle is 1nm or more 100nm or less.
Specifically, whole blood instills in loading slot, realizes that separation obtains serum by the hemofiltration film, serum is in the raceway groove
It is interior quantitatively to be mixed with dilution.
For the whole blood immunity detection device of this programme compared with traditional detection device, advantage is can be directly by finger tip blood
Or the whole bloods such as venous blood, it is added in the micro-fluidic chip, by hemofiltration film, realizes that whole blood separation obtains serum, then distribute
To instrument connection.Whole process is easy to operate, to user it is professional require it is low, it is right without getting out serum or blood plasma in advance
It only needs to understand how fetching point blood or venous blood for user.
Local surface plasma resonance effect (Localized of the principle of this programme based on nanogold particle
Surface Plasmon Resonance, abbreviation LSPR effect), the most significant feature of the principle is that of avoiding pervious enzyme-linked
The methods of immunization or radio-labeled need the antibody for adding catalyzing enzyme and color developing agent or the same position of radioactivity during the test
The operations such as element.It entirely uses process without adding any marker using what this programme was detected, whole blood test sample is added
Afterwards, the disease markers detected in sample can be tied with the antibody as detection probe in the instrument connection of micro-fluidic chip
It closes, causes the environmental change around nanogold particle, change to the absorption of light so as to cause nanogold particle, therefore is logical
Cross analysis absorption peak variation, can directly test antibody concentration, be based on such principle so that test process letter
Just, the consumptive material for testing needs is less, to which cost of implementation reduces.
Specifically, the chip based on LSPR effects can utilize the specific reaction of analysans direct in detection process
Realize qualitative, the quantitative measurment to analysans.When to detect certain analysans, can first will with it is corresponding to be analyzed
The substance that object is specifically bound is modified as detection probe between nanogold particle surface or nanogold particle, containing needing
The sample of analyte can be specifically bound after entering instrument connection with detection probe, and detecting and controlling system is provided with according to transmission
Whether the delustring of the chip of nanogold changes, you can to analyze in sample containing analysans or containing analysans
Amount.
Currently, chip is in the interaction based on protein-protein or protein-DNA, strep-biotin
Reaction, immunoglobulin G (Immunoglobulin G, abbreviation IgG) test or Ag-Ab interaction in terms of inspection
Survey has application, wherein protide antigen-antibody can be used for detecting various diseases, can be by detecting disease in disease application
Sick marker judges whether to suffer from disease.
Disease markers in disease detection include tumour class, heart infarction class, hepatopathy inflammation class, infect class, brain
Obstruct class, immune function class etc., wherein tumour class specifically includes lung cancer, liver cancer, gastric cancer, cancer of pancreas, intestinal cancer, and breast cancer is preceding
Row gland cancer etc.；Heart infarction class includes Troponin I, troponin T, myoglobins, creatine hormone isodynamic enzyme, N-terminal b-type natriuretic peptide
Precursor etc.；Hepatopathy inflammation class includes Hepatitis B virus and antibody of HCV and core antigen of C type hepatitis virus；Infection
Class includes c reactive protein, Procalcitonin, interleukin 6 etc..
Different types of disease has different specific disease markers, can will when needing to detect certain disease
The antibody specifically bound with certain specific disease markers is as detection probe.Such as when detecting liver cancer, need to examine
Disease markers alpha-fetoprotein (α-fetoprotein, abbreviation α FP or AFP) is surveyed, AFP antibody can be used as detection probe；
It, can be using adalimumab as detection probe when needing to detect disease markers Tumor necrosis factor TNF-a；In detection hepatitis B
When, it needs to detect disease markers hepatitis B surface antigen (HBsAg), it can be with hepatitis B surface antibody (HBsAb) for detection probe；It needs
It, can be with hepatitis B e antibody (HBeAb) for detection probe when detecting disease markers hepatitis B virus e antigen (HBeAg)；In detection mammary gland
When cancer, need to detect disease markers human breast carcinoma CA15-3, it can be using human breast carcinoma CA153 antibody as detection probe；It is detecting
When herpesvirus infection, needs to detect disease markers Epstein-Barr viruses (EBV), can be with EBV-CA (IgM) antibody
Detection probe.As it can be seen that multiple instrument connections are arranged on chip and are modified on the nanogold particle of different instrument connections different
Antibody is as detection probe, you can and it realizes and detects a variety of different diseases simultaneously, make detection more efficiently and conveniently, suitable family
Front yard, community, hospital use.
As a kind of perferred technical scheme, the parallel optical assembly includes light source, incoming optical fiber and incoming collimator, institute
It states light source to connect by the incoming optical fiber with the incoming collimator, it is separate that the incoming collimator is mounted on the instrument connection
The direction of the launch of one end of the spectral analysis module, the incoming collimator is directed at the instrument connection.
Specifically, for the light source for sending out continuous visible white light, wave-length coverage is 380nm or more 800nm hereinafter, described
Light source can be halogen lamp.
Specifically, carrier of the incoming optical fiber as light conduction realizes that light is conducted from the light source to the biography
Enter collimator.
Specifically, the incoming collimator is used to prepare parallel rays, and the parallel rays is emitted to the instrument connection.
As a kind of perferred technical scheme, the spectral analysis module includes spectrometer, outflow optical fiber and outflow collimation
Device, the spectrometer are connect with the outflow collimator by the outflow optical fiber, and the outflow collimator is mounted on the survey
The reception direction of the one end of prospect hole far from the parallel optical assembly, the outflow collimator is directed at the instrument connection.
Specifically, instrument connection, the outflow described in the incoming collimator-alignment when the parallel optical assembly transmitting light
Instrument connection described in collimator-alignment, the axis of the incoming collimator, the axis of the outflow collimator and the instrument connection
Axis is located on the same line.
Preferably, the incoming collimator is located at the surface of the instrument connection, and the outflow collimator is located at the survey
The underface of prospect hole.
Preferably, the incoming collimator is located at the underface of the instrument connection, and the outflow collimator is located at the survey
The surface of prospect hole.
Specifically, the outflow collimator is used to receive the light by after the instrument connection.
Specifically, the carrier of the outflow optical fiber as light conduction, realization light from the outflow collimator conduct to
The spectrometer.
Specifically, the spectrometer forms Spectroscopic analysis results by the Output Signal Analysis of the outflow optical fiber, after being
Continuous health analysis provides data and supports.
The structure of the whole blood immunity detection device of this programme is simple, using common optical fiber as conductive medium, without multiple
Miscellaneous light path design keeps the structure of device compacter, is conducive to the overall volume for reducing device, is suitble to family, community, hospital
It uses.
As a kind of perferred technical scheme, the instrument connection runs through the micro-fluidic chip, the bottom of the instrument connection
It is provided with transparent substrate, the nanogold particle and the detection probe are placed on the transparent substrate.
Preferably, the transparent substrate is sheet glass or quartz plate or sheet of transparent ceramic or transparent polymer sheet etc..
Preferably, the nanogold particle is spacedly distributed on the transparent substrate, and the detection probe is distributed in institute
State the interstitial site between nanogold particle.
Preferably, the nanogold particle is spacedly distributed on the transparent substrate, and the detection probe is arranged in institute
State the surface of nanogold particle.
As a kind of perferred technical scheme, solution inlet is offered on the measurement wall of the instrument connection, the solution enters
Mouth is connected to the raceway groove.
Specifically, described to measure the hole wall that wall is the instrument connection.
As a kind of perferred technical scheme, further include man-machine interaction device, the man-machine interaction device respectively with it is described parallel
Optical assembly, spectral analysis module electrical connection.
Preferably, the man-machine interaction device is electrically connected with the light source, the spectrometer respectively.
Preferably, the man-machine interaction device includes display screen, the spectrum point for showing the spectral analysis module output
Analyse result.
Further, the man-machine interaction device is touching display screen, for controlling the parallel optical assembly and the spectrum
Analytic unit, and display Spectroscopic analysis results.
As a kind of perferred technical scheme, the man-machine interaction device includes bluetooth devices and/or WIFI transceiving devices, is used
In carrying out data exchange with external electronic equipment.
Specifically, the output signal of the outflow optical fiber carries out analysis by the man-machine interaction device and forms spectrum analysis knot
Fruit, or analysis is further carried out by external electronic equipment and forms more perfect Spectroscopic analysis results.
As a kind of perferred technical scheme, the quantity of the instrument connection is 1-100.
As a kind of perferred technical scheme, the quantity of the instrument connection is 1-10.
Preferably, the quantity of the instrument connection is 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9
Or 10 or 20 or 50 or 100.
Preferably, the quantity of the incoming collimator is equal with the quantity of the instrument connection, the number of the outflow collimator
Amount is equal with the quantity of the instrument connection, and there are one the incoming collimator, Mei Gesuo for one end setting of each instrument connection
The other end setting of instrument connection is stated there are one the outflow collimator.
As a kind of perferred technical scheme, the instrument connection is spacedly distributed at the edge of the micro-fluidic chip, often
It is connected to by a raceway groove between a instrument connection and the loading slot.
The whole blood immunity detection device of this programme use process is as follows：
The whole blood 1-3 drops (about 100 μ L) and dilution for taking human body first are added to the sample-adding of the micro-fluidic chip
In slot, wherein hemofiltration film is provided with micropore, and the aperture of micropore can be 7 μm hereinafter, so that serum passes through, and by haemocyte etc.
Large particle is trapped in loading slot one end, to realize that whole blood detaches.Serum after separation enters in raceway groove, and in channels
It is mixed with dilution, quantitatively enters the instrument connection.Into the instrument connection serum mixed liquor can be fixed on it is described transparent
Detection probe on substrate is specifically bound.Then the light source sends out a branch of continuous light, by the incoming optical fiber and
The incoming collimator enters the instrument connection in the form of directional light, since load has the transparent substrate of nanogold particle to send out
Raw surface plasmon resonance effect (abbreviation LSPR effects), changes the intensity and peak position of emergent ray, by described
Emergent ray after transparent substrate is received by the outflow collimator, and is divided by the outflow fiber optic conduction to spectrometer
Analysis forms Spectroscopic analysis results, and gained Spectroscopic analysis results are showed by the display of the man-machine interaction device, or into one
Spectroscopic analysis results are transferred to external electronic equipment and carry out subsequent analysis by step.
The beneficial effects of the utility model are：A kind of whole blood immunity detection device is provided, is filtered by being arranged in loading slot
Blood film, and nanogold particle and detection probe are set in detection hole, allow the device that whole blood is directly added to the miniflow
It controls in chip, realizes that whole blood separation obtains serum by the hemofiltration film, then arrive instrument connection, whole process is easy to operate, right
User it is professional require it is low.And LSPR effect of the principle of this programme based on nanogold particle, it is examined using this programme
It surveys without adding any marker, simple testing process, the consumptive material for testing needs is less, and use cost is low.
Description of the drawings
The utility model is described in further detail below according to drawings and examples.
The structural schematic diagram of Fig. 1 whole blood immunity detection devices described in embodiment 1；
Fig. 2 is the structural schematic diagram of instrument connection described in embodiment 1；
Fig. 3 is the structural schematic diagram of the whole blood immunity detection device described in embodiment 2.
In Fig. 1 to Fig. 3：
1, micro-fluidic chip；2, hemofiltration film；3, loading slot；4, instrument connection；41, wall is measured；42, solution inlet；5, transparent
Substrate；6, nanogold particle；7, detection probe；8, light source；9, it is passed to optical fiber；10, it is passed to collimator；11, spectrometer；12, it passes
Go out optical fiber；13, collimator is spread out of；14, man-machine interaction device.
Specific implementation mode
Further illustrate the technical solution of the utility model below with reference to the accompanying drawings and specific embodiments.
Embodiment 1
A kind of whole blood immunity detection device, as depicted in figs. 1 and 2, including micro-fluidic chip 1, on the micro-fluidic chip 1
It is provided with loading slot 3, instrument connection 4 and raceway groove, hemofiltration film 2, one end connection of the hemofiltration film 2 are provided in the loading slot 3
Loading slot 3, the other end of the hemofiltration film 2 far from loading slot 3 are connected to raceway groove, and the loading slot 3 is opened in the micro-fluidic core
The central region of piece 1, the instrument connection 4 are opened in the edge of the micro-fluidic chip 1, and the quantity of the instrument connection 4 is 1,
Solution inlet 42 is offered on the measurement wall 41 of the instrument connection 4, the solution inlet 42 is connected to the raceway groove, the test
Nanogold particle 6 and detection probe 7 are provided in hole 4, one end of the instrument connection 4 is provided with parallel optical assembly, the test
The other end in hole 4 is provided with spectral analysis module.
In this present embodiment, the micro-fluidic chip 1 is the thin rounded flakes for having composite construction, and the composite construction includes
The main layer of main layer and auxiliary layer, the micro-fluidic chip 1 is made of stiff polymer material, the micro-fluidic chip 1 it is auxiliary
Layer is helped to be made of elastic material.The raceway groove is formed between main layer and auxiliary layer, described in the position of the correspondence raceway groove
Side of the auxiliary layer far from the raceway groove is provided with valve, and the valve is 1 or more, is arranged at equal intervals on the raceway groove.
Auxiliary layer elastic material is squeezed when valve opening makes raceway groove be closed, it is possible thereby to which the liquid in raceway groove is driven to flow.The survey
The shape of prospect hole 4 is cylindrical, and the diameter of the nanogold particle 6 is 50nm.In other embodiments, the micro-fluidic core
Piece 1 can be the shapes such as rectangular or diamond shape or ellipse or triangle, and the shape of the instrument connection 4 can be rectangular or diamond shape or ellipse
Circle or triangle, the diameter of the nanogold particle 6 can be 1nm or 100nm or 1nm to the arbitrary value between 100nm.
Whole blood can be directly added in the micro-fluidic chip 1 by the whole blood immunity detection device, pass through the hemofiltration film 2
Realize whole blood separation obtain serum, be then dispensed for instrument connection 4, whole process is easy to operate, to user it is professional require it is low.
And LSPR effect of the principle of this programme based on nanogold particle 6, it is detected using this programme without adding any label
Object, simple testing process, the consumptive material for testing needs is less, and use cost is low.
In this present embodiment, the parallel optical assembly includes light source 8, incoming optical fiber 9 and incoming collimator 10, the light source
8 are connect with the incoming collimator 10 by the incoming optical fiber 9, and it is remote that the incoming collimator 10 is mounted on the instrument connection 4
The direction of the launch of one end from the spectral analysis module, the incoming collimator 10 is directed at the instrument connection 4.The light source 8
For sending out continuous visible white light, wave-length coverage is 380nm or more 800nm hereinafter, the light source 8 is halogen lamp.It is described incoming
Carrier of the optical fiber 9 as light conduction realizes that light is conducted from the light source 8 to the incoming collimator 10.The incoming standard
Straight device 10 is used to prepare parallel rays, and the parallel rays is emitted to the instrument connection 4.
In this present embodiment, the spectral analysis module includes spectrometer 11, outflow optical fiber 12 and outflow collimator 13, institute
It states spectrometer 11 to connect by the outflow optical fiber 12 with the outflow collimator 13, the outflow collimator 13 is mounted on described
The reception direction of the one end of instrument connection 4 far from the parallel optical assembly, the outflow collimator 13 is directed at the instrument connection 4.Institute
Outflow collimator 13 is stated to be used to receive the light by after the instrument connection 4.Load of the outflow optical fiber 12 as light conduction
Body realizes that light is conducted from the outflow collimator 13 to the spectrometer 11.The spectrometer 11 passes through the outflow optical fiber
12 Output Signal Analysis forms Spectroscopic analysis results, and providing data for the analysis of follow-up health supports.The whole blood immunity is examined
Survey device keeps the structure of device compacter, favorably as conductive medium using common optical fiber without complicated light path design
In the overall volume for reducing device, family, community, hospital is suitble to use.
The incoming collimator 10 is directed at the instrument connection 4, the outflow collimation when the parallel optical assembly transmitting light
Device 13 is directed at the instrument connection 4, the axis of the incoming collimator 10, the axis of the outflow collimator 13 and the test
The axis in hole 4 is located on the same line.In this present embodiment, the incoming collimator 10 be located at the instrument connection 4 just on
Side, the outflow collimator 13 are located at the underface of the instrument connection 4.In other embodiments, the incoming collimator 10
In the underface of the instrument connection 4, the outflow collimator 13 is located at the surface of the instrument connection 4.
In this present embodiment, the instrument connection 4 runs through the micro-fluidic chip 1, and the bottom of the instrument connection 4 is provided with
Bright substrate 5, the nanogold particle 6 and the detection probe 7 are placed on the transparent substrate 5.Specifically, the nanogold
Particle 6 is spacedly distributed on the transparent substrate 5, and the detection probe 7 is distributed in the gap between the nanogold particle 6
Position.In this present embodiment, the transparent substrate 5 is sheet glass.In other embodiments, the transparent substrate 5 can also be
Quartz plate or sheet of transparent ceramic or transparent polymer sheet.In other embodiments, the instrument connection 4 is blind hole, the nanogold
Particle 6 and the detection probe 7 are placed on the bottom of the blind hole.In other embodiments, between the nanogold particle 6 is equal
Every being distributed on the transparent substrate 5, the detection probe 7 is arranged on the surface of the nanogold particle 6.
In this present embodiment, which further includes man-machine interaction device 14, and the man-machine interaction device 14 divides
It is not electrically connected with the light source 8, the spectrometer 11.The man-machine interaction device 14 is touching display screen, described flat for controlling
Row optical assembly and the spectral analysis module, and display Spectroscopic analysis results.The man-machine interaction device 14 includes bluetooth devices
With WIFI transceiving devices, it is used to carry out data exchange with external electronic equipment.Specifically, the output of the outflow optical fiber 12
Signal carries out analysis by the man-machine interaction device 14 and forms Spectroscopic analysis results, or is further carried out by external electronic equipment
Analysis forms more perfect Spectroscopic analysis results.
The whole blood immunity detection device of this programme use process is as follows：
The whole blood 1-3 drops (about 100 μ L) and dilution for taking human body first are added to the sample-adding of the micro-fluidic chip 1
In slot 3, wherein hemofiltration film 2 is provided with micropore, and the aperture of micropore is 7 μm, so that serum passes through, and by bulky grains such as haemocytes
Object is trapped in 3 one end of loading slot, to realize that whole blood detaches.Serum after separation enters in raceway groove, and in channels with it is dilute
Liquid mixing is released, the instrument connection 4 is quantitatively entered.Into the instrument connection 4 serum mixed liquor can be fixed on the transparent base
Detection probe 7 on piece 5 is specifically bound.Then the light source 8 sends out a branch of continuous light, passes through the incoming optical fiber 9
Enter the instrument connection 4 in the form of directional light with the incoming collimator 10, since load has the described transparent of nanogold particle 6
Surface plasmon resonance effect (abbreviation LSPR effects) occurs for substrate 5, changes the intensity and peak position of emergent ray, leads to
The emergent ray crossed after the transparent substrate 5 is received by the outflow collimator 13, and extremely by the outflow optical fiber 12 conduction
Spectrometer 11 carries out analysis and forms Spectroscopic analysis results, the display that gained Spectroscopic analysis results pass through the man-machine interaction device 14
It shows, or Spectroscopic analysis results is further transferred to external electronic equipment and carry out subsequent analysis.
Embodiment 2
The present embodiment and embodiment 1 difference lies in：
As shown in figure 3, the quantity of the instrument connection 4 is 2, the instrument connection 4 is spacedly distributed in the micro-fluidic core
The edge of piece 1 is each connected to by a raceway groove between the instrument connection 4 and the loading slot 3.The incoming standard
The quantity of straight device 10 is equal with the quantity of the instrument connection 4, the quantity of the quantity and the instrument connection 4 of the outflow collimator 13
Equal, there are one the incoming collimator 10, the other ends of each instrument connection 4 for one end setting of each instrument connection 4
There are one the outflow collimators 13 for setting.By the way that multiple measured holes are arranged, multigroup Spectroscopic analysis results, energy can be formed
It is enough exclude it is individual abnormal as a result, to improve the reliability of detection.In other embodiments, the quantity of the instrument connection 4 can
To be 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10.
It is to be understood that above-mentioned specific implementation mode is only the preferred embodiment and institute's application technology of the utility model
Principle, in technical scope disclosed in the utility model, what any one skilled in the art was readily apparent that
Change or replacement should all be covered within the protection scope of the present utility model.
Claims (10)
1. a kind of whole blood immunity detection device, including micro-fluidic chip, which is characterized in that include sample-adding on the micro-fluidic chip
Slot, instrument connection and raceway groove, the loading slot are connected to the instrument connection by the raceway groove, and hemofiltration is provided in the loading slot
Film is provided with nanogold particle and detection probe in the instrument connection, and one end of the instrument connection is provided with parallel optical assembly, institute
The other end for stating instrument connection is provided with spectral analysis module.
2. a kind of whole blood immunity detection device according to claim 1, which is characterized in that the parallel optical assembly includes light
Source, incoming optical fiber and incoming collimator, the light source are connect with the incoming collimator by the incoming optical fiber, described incoming
Collimator is mounted on the one end of the instrument connection far from the spectral analysis module, the direction of the launch direction of the incoming collimator
The instrument connection.
3. a kind of whole blood immunity detection device according to claim 1, which is characterized in that the spectral analysis module includes
Spectrometer, outflow optical fiber and outflow collimator, the spectrometer are connect with the outflow collimator by the outflow optical fiber, institute
It states outflow collimator and is mounted on the one end of the instrument connection far from the parallel optical assembly, the reception direction of the outflow collimator
Towards the instrument connection.
4. a kind of whole blood immunity detection device according to claim 1, which is characterized in that the instrument connection is through described micro-
The bottom of fluidic chip, the instrument connection is provided with transparent substrate, and the nanogold particle and the detection probe are placed on institute
It states on transparent substrate.
5. a kind of whole blood immunity detection device according to claim 1, which is characterized in that further include man-machine interaction device, institute
Man-machine interaction device is stated to be electrically connected with the parallel optical assembly, the spectral analysis module respectively.
6. a kind of whole blood immunity detection device according to claim 5, which is characterized in that the man-machine interaction device includes indigo plant
Tooth device and/or WIFI transceiving devices, for carrying out data exchange with external electronic equipment.
7. a kind of whole blood immunity detection device according to claim 1, which is characterized in that be provided on the hemofiltration film micro-
Hole, the micropore size are 7 μm or less.
8. a kind of whole blood immunity detection device according to claim 1, which is characterized in that the micro-fluidic chip is compound
Layer structure, the lamination layer structure are divided into main layer and auxiliary layer, and the main layer is made of hard material, and auxiliary layer is using elasticity
Material is made, and the raceway groove is formed between main layer and auxiliary layer structure.
9. according to a kind of whole blood immunity detection device of claim 1 to 8 any one of them, which is characterized in that the instrument connection
Quantity be 1-100.
10. a kind of whole blood immunity detection device according to claim 9, which is characterized in that the instrument connection divides at equal intervals
Cloth is each connected by a raceway groove between the instrument connection and the loading slot at the edge of the micro-fluidic chip
It is logical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721800206.8U CN207689495U (en) | 2017-12-21 | 2017-12-21 | A kind of whole blood immunity detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721800206.8U CN207689495U (en) | 2017-12-21 | 2017-12-21 | A kind of whole blood immunity detection device |
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| Publication Number | Publication Date |
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| CN207689495U true CN207689495U (en) | 2018-08-03 |
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| CN (1) | CN207689495U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109946445A (en) * | 2017-12-21 | 2019-06-28 | 东莞东阳光科研发有限公司 | A kind of whole blood immunity detection device |
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2017
- 2017-12-21 CN CN201721800206.8U patent/CN207689495U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109946445A (en) * | 2017-12-21 | 2019-06-28 | 东莞东阳光科研发有限公司 | A kind of whole blood immunity detection device |
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