CN103497048A - Antiviral nano aquiculture fertilizer and preparation method thereof - Google Patents
Antiviral nano aquiculture fertilizer and preparation method thereof Download PDFInfo
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- CN103497048A CN103497048A CN201310426803.9A CN201310426803A CN103497048A CN 103497048 A CN103497048 A CN 103497048A CN 201310426803 A CN201310426803 A CN 201310426803A CN 103497048 A CN103497048 A CN 103497048A
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Abstract
The invention discloses an antiviral nano aquiculture fertilizer and a preparation method thereof. The aquiculture fertilizer comprises Bacillus subtilis spore, Bacillus licheniformis, lactein, rapeseed oil dreg, oyster powder, carrier, sucrose, compound sodium nitrophenolate and sodium humate. The preparation method of the aquiculture fertilizer comprises the following steps: (A) selecting strains of the Bacillus subtilis spore, Bacillus licheniformis and lactein; (B) mixing the rapeseed oil dreg, carrier, sucrose, compound sodium nitrophenolate and sodium humate; (C) mixing the filter residue with the oyster powder; (D) mixing the powder in the step (A) with the mixed oyster powder in the step (C); and (E) fermenting and drying. By using the rapeseed oil dreg as the main component, the aquiculture fertilizer solves the problem of outlet of the rapeseed oil dreg production waste, and promotes the healthy and sustainable development of green aquiculture; and the aquiculture fertilizer has the advantages of quick effect taking and enduring action time, and has the antimicrobial and disinfecting functions.
Description
technical field:
The present invention relates to fertilizer and preparation method thereof, be specifically related to nanometer antiviral aquaculture fertilizer and preparation method thereof, belong to technical field of aquaculture.
background technology:
In order to obtain higher output in certain water body, people have to drop into a large amount of fertilizer in water body, with the material that meets the aquatic animal growth and the requirement of controlling disease.This take raise by force breeding way that heavy fertilizer is feature and strengthened water body nitrogen, phosphorus, organic substance decomposing and transform load.Again because being negligent of regulating and controlling water quality and management, it is not smooth that microorganism is decomposed link, in water body, material cycle and flow of energy are obstructed, and residual bait, organic residue constantly accumulate, so that NH4+ (NH3), NO2-, H2S, equal size are far beyond the aquaculture water water quality standard of national regulation.Lake, Reservoir Eutrophication are serious, and the blue-green algae wawter bloom is wreaked havoc, and not only local resident's productive life are produced to greatly impact, but also bring a series of ecological environment problem.
Existing employing chemical fertilizer carries out the water body cultivation, and its nutritive element is single, although water body is cultivated comparatively fast, the time length is very short, and after the nutritive element in water body runs out of, what water body again can be very fast is thin.
In addition, existing aquaculture fertilizer does not generally have the antibiotic and sterilizing function, so, when adding fertilizer, also need to add antibiosis and sterilization agent, thereby greatly strengthened user's workload.
summary of the invention:
The present invention seeks to, be used for making up the deficiencies in the prior art, and nanometer antiviral aquaculture fertilizer of rapid-action, action time of lasting, environmental protection and preparation method thereof is provided.
To achieve these goals, technical scheme of the present invention is as follows:
Nanometer antiviral aquaculture fertilizer, is characterized in that,
Comprise that spores of bacillus subtilin element, Bacillus licheniformis are plain, lactobacillin, the vegetable seed dregs of fat, ostreae testa pulverata, carrier, sucrose, nano controlled-release mixture, sodium nitrophenolate and sodium humate;
Described carrier comprises rice bran, wheat bran and peanut press pulp;
Described spores of bacillus subtilin is plain, Bacillus licheniformis is plain and the mass ratio of lactobacillin and the vegetable seed dregs of fat is 0.04～0.06:1;
The mass ratio of described carrier and the vegetable seed dregs of fat is 0.5～0.8:1;
The mass ratio of described sucrose and the vegetable seed dregs of fat is 0.06～0.08:1;
The mass ratio of described sodium nitrophenolate and sodium humate and the vegetable seed dregs of fat is 0.07～0.09:1;
The mass ratio of described nano controlled-release mixture and the vegetable seed dregs of fat is 0.1～0.2:1;
The mass ratio of described ostreae testa pulverata and the vegetable seed dregs of fat is 0.1～0.2:1.
1 described nanometer antiviral aquaculture fertilizer, is characterized in that,
The described described nano controlled-release mixture preparation method that receives is: (1) is by the silicon hydroxyapatite, Attapulgite, hydrotalcite, polynite, titanium dioxide, calcite, kaolin mixes according to the mass fraction proportioning of 35-45:10-15: 10-15:8-10:8-10:7-8:5-7, join in the mixing solutions of 230 parts of methyl-sulphoxides (DMSO) and 50 parts of methyl alcohol, in 65 ℃, stir 60 hours, filter, and wash and remove excessive methyl-sulphoxide (DMSO) for 3 times with the hot ethanol of 60 ℃ of temperature, put into vacuum drying oven, dry 24 hours of 60 ℃ of temperature, grinding is sieved, obtain a modified composite,
(2) 1 part of modified composite, 15 parts of Potassium ethanoates and 25 parts of distilled water are mixed, in temperature 50 C, stir more than 10 hours, in 30 ℃ of temperature, first under ultrasonic electric power 350W condition, disperse 3.5 hours, and then disperse 4 hours under ultrasonic electric power 250W condition.Filter, and wash 3 times with distillation, 80 ℃ of vacuum-drying 24 hours, grinding is sieved, and obtains twice-modified mixture; (3) above-mentioned 1 part of twice-modified mixture is disperseed to 60min at the 600W power ultrasonic, with constant temperature heating device, be heated to 90 ℃, and stir (1200r/min) 60 minutes with mechanical stirrer; Obtain the nano controlled-release carrier complexes.
The preparation method of described nanometer antiviral aquaculture fertilizer, is characterized in that, comprises following step:
(A) spores of bacillus subtilin element, Bacillus licheniformis is plain, lactobacillin is selected bacterial classification, and carry out respectively 25 ℃ and 36 ℃ of separation and Culture lawn is extracted to purifying, and by 18 ℃ of cryodrying powdereds of purification thing;
(B) by the vegetable seed dregs of fat, carrier, sucrose, nano controlled-release carrier complexes, sodium nitrophenolate and sodium humate, the mass ratio according to claim 1 requires to send in stirring mixer to be uniformly mixed, and is ground into powder by biotechnology Pengization;
(C) filter residue step (B) obtained is mixed with ostreae testa pulverata, and sends moisture eliminator to and carry out drying, obtains mixing ostreae testa pulverata, and the Temperature Setting of described moisture eliminator is 120～150 ℃, and be 12～17 minutes time of drying;
(D) the mixing ostreae testa pulverata mixing and stirring that powder step (A) obtained and step (C) obtain;
(E) mixed powder after being uniformly mixed is crossed to 38 mesh sieves, then send in fermentation vat and fermented, the temperature in fermentation vat is controlled under 22～29 ℃, ferment 7～10 days, finally the liquid after fermentation is filtered to drying;
Obtain nanometer antiviral aquaculture fertilizer of the present invention.
Beneficial effect of the present invention is as follows:
The main component of nanometer antiviral aquaculture fertilizer of the present invention is the vegetable seed dregs of fat, solved the problem of outlet that the vegetable seed dregs of fat are produced waste, promoted again the sustainable health development of green culture fishery, the real recycling economy turned waste into wealth that realizes, have good economic results in society; In addition, the present invention fills a prescription rationally, and the preparation method is simple, uses product application of the present invention in water body, and through actual verification, product of the present invention is rapid-action, and action time is lasting, harmless environmental protection.Aquaculture fertilizer of the present invention has the antibiotic and sterilizing function, and antibiotic and sterilizing is effective, eliminated to human body and the cultivation species poisonous, water quality and environment are had to the harm such as pollution, the operation using method easy, can apply widely.The nano controlled-release additive has good biocompatibility, gastrointestinal tract mucous affinity, each component that makes it load has controlled-release function, through twice-modified nano-complex drug loading and adsorptive capacity, all improve a lot, the fertilizer efficiency time lengthening is more than 3 months, effectively suppress the increment of animal pathogen, improve the producer's benefit.
embodiment:
For technique means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, further set forth the present invention.
The nanometer antiviral aquaculture fertilizer of the present embodiment,
Nanometer antiviral aquaculture fertilizer, is characterized in that,
Comprise that spores of bacillus subtilin element, Bacillus licheniformis are plain, lactobacillin, the vegetable seed dregs of fat, ostreae testa pulverata, carrier, sucrose, nano controlled-release mixture, sodium nitrophenolate and sodium humate;
Described carrier comprises rice bran, wheat bran and peanut press pulp;
Described spores of bacillus subtilin is plain, Bacillus licheniformis is plain and the mass ratio of lactobacillin and the vegetable seed dregs of fat is 0.04～0.06:1;
The mass ratio of described carrier and the vegetable seed dregs of fat is 0.5～0.8:1;
The mass ratio of described sucrose and the vegetable seed dregs of fat is 0.06～0.08:1;
The mass ratio of described sodium nitrophenolate and sodium humate and the vegetable seed dregs of fat is 0.07～0.09:1;
The mass ratio of described nano controlled-release mixture and the vegetable seed dregs of fat is 0.1～0.2:1;
The mass ratio of described ostreae testa pulverata and the vegetable seed dregs of fat is 0.1～0.2:1.
Described nanometer antiviral aquaculture fertilizer, is characterized in that,
The described described nano controlled-release mixture preparation method that receives is: (1) is by the silicon hydroxyapatite, Attapulgite, hydrotalcite, polynite, titanium dioxide, calcite, kaolin mixes according to the mass fraction proportioning of 35-45:10-15: 10-15:8-10:8-10:7-8:5-7, join in the mixing solutions of 230 parts of methyl-sulphoxides (DMSO) and 50 parts of methyl alcohol, in 65 ℃, stir 60 hours, filter, and wash and remove excessive methyl-sulphoxide (DMSO) for 3 times with the hot ethanol of 60 ℃ of temperature, put into vacuum drying oven, dry 24 hours of 60 ℃ of temperature, grinding is sieved, obtain a modified composite,
(2) 1 part of modified composite, 15 parts of Potassium ethanoates and 25 parts of distilled water are mixed, in temperature 50 C, stir more than 10 hours, in 30 ℃ of temperature, first under ultrasonic electric power 350W condition, disperse 3.5 hours, and then disperse 4 hours under ultrasonic electric power 250W condition.Filter, and wash 3 times with distillation, 80 ℃ of vacuum-drying 24 hours, grinding is sieved, and obtains twice-modified mixture; (3) above-mentioned 1 part of twice-modified mixture is disperseed to 60min at the 600W power ultrasonic, with constant temperature heating device, be heated to 90 ℃, and stir (1200r/min) 60 minutes with mechanical stirrer; Obtain the nano controlled-release carrier complexes.
The preparation method of described nanometer antiviral aquaculture fertilizer, is characterized in that, comprises following step:
(A) spores of bacillus subtilin element, Bacillus licheniformis is plain, lactobacillin is selected bacterial classification, and carry out respectively 25 ℃ and 36 ℃ of separation and Culture lawn is extracted to purifying, and by 18 ℃ of cryodrying powdereds of purification thing;
(B) by the vegetable seed dregs of fat, carrier, sucrose, nano controlled-release carrier complexes, sodium nitrophenolate and sodium humate, the mass ratio according to claim 1 requires to send in stirring mixer to be uniformly mixed, and is ground into powder by biotechnology Pengization;
(C) filter residue step (B) obtained is mixed with ostreae testa pulverata, and sends moisture eliminator to and carry out drying, obtains mixing ostreae testa pulverata, and the Temperature Setting of described moisture eliminator is 120～150 ℃, and be 12～17 minutes time of drying;
(D) the mixing ostreae testa pulverata mixing and stirring that powder step (A) obtained and step (C) obtain;
(E) mixed powder after being uniformly mixed is crossed to 38 mesh sieves, then send in fermentation vat and fermented, the temperature in fermentation vat is controlled under 22～29 ℃, ferment 7～10 days, finally the liquid after fermentation is filtered to drying;
Obtain nanometer antiviral aquaculture fertilizer of the present invention.
The main component of nanometer antiviral aquaculture fertilizer of the present invention is the vegetable seed dregs of fat, solved the problem of outlet that the vegetable seed dregs of fat are produced waste, promoted again the sustainable health development of green culture fishery, the real recycling economy turned waste into wealth that realizes, have good economic results in society; In addition, the present invention fills a prescription rationally, and the preparation method is simple, uses product application of the present invention in water body, and through actual verification, product of the present invention is rapid-action, and action time is lasting, harmless environmental protection.Aquaculture fertilizer of the present invention has the antibiotic and sterilizing function, and antibiotic and sterilizing is effective, eliminated to human body and the cultivation species poisonous, water quality and environment are had to the harm such as pollution, the operation using method easy, can apply widely.The nano controlled-release additive has good biocompatibility, gastrointestinal tract mucous affinity, each component that makes it load has controlled-release function, through twice-modified nano-complex drug loading and adsorptive capacity, all improve a lot, the fertilizer efficiency time lengthening is more than 3 months, effectively suppress the increment of animal pathogen, improve the producer's benefit.
Above demonstration and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof
.
Claims (4)
1. nanometer antiviral aquaculture fertilizer, is characterized in that,
Comprise that spores of bacillus subtilin element, Bacillus licheniformis are plain, lactobacillin, the vegetable seed dregs of fat, ostreae testa pulverata, carrier, sucrose, nano controlled-release mixture, sodium nitrophenolate and sodium humate;
Described carrier comprises rice bran, wheat bran and peanut press pulp;
Described spores of bacillus subtilin is plain, Bacillus licheniformis is plain and the mass ratio of lactobacillin and the vegetable seed dregs of fat is 0.04～0.06:1;
The mass ratio of described carrier and the vegetable seed dregs of fat is 0.5～0.8:1;
The mass ratio of described sucrose and the vegetable seed dregs of fat is 0.06～0.08:1;
The mass ratio of described sodium nitrophenolate and sodium humate and the vegetable seed dregs of fat is 0.07～0.09:1;
The mass ratio of described nano controlled-release mixture and the vegetable seed dregs of fat is 0.1～0.2:1;
The mass ratio of described ostreae testa pulverata and the vegetable seed dregs of fat is 0.1～0.2:1.
2. nanometer antiviral aquaculture fertilizer according to claim 1, is characterized in that,
Described nano controlled-release mixture preparation method is: (1) is by the silicon hydroxyapatite, Attapulgite, hydrotalcite, polynite, titanium dioxide, calcite, kaolin mixes according to the mass fraction proportioning of 35-45:10-15: 10-15:8-10:8-10:7-8:5-7, join in the mixing solutions of 230 parts of methyl-sulphoxides (DMSO) and 50 parts of methyl alcohol, in 65 ℃, stir 60 hours, filter, and wash and remove excessive methyl-sulphoxide (DMSO) for 3 times with the hot ethanol of 60 ℃ of temperature, put into vacuum drying oven, dry 24 hours of 60 ℃ of temperature, grinding is sieved, obtain a modified composite,
(2) 1 part of modified composite, 15 parts of Potassium ethanoates and 25 parts of distilled water are mixed, in temperature 50 C, stir more than 10 hours, in 30 ℃ of temperature, first under ultrasonic electric power 350W condition, disperse 3.5 hours, and then disperse 4 hours under ultrasonic electric power 250W condition.
3. filter, and wash 3 times with distillation, 80 ℃ of vacuum-drying 24 hours, grinding is sieved, and obtains twice-modified mixture; (3) above-mentioned 1 part of twice-modified mixture is disperseed to 60min at the 600W power ultrasonic, with constant temperature heating device, be heated to 90 ℃, and stir (1200r/min) 60 minutes with mechanical stirrer; Obtain the nano controlled-release carrier complexes.
4. according to the preparation method of claim 1 or the described nanometer antiviral aquaculture of 2 any one fertilizer, it is characterized in that, comprise following step:
(A) spores of bacillus subtilin element, Bacillus licheniformis is plain, lactobacillin is selected bacterial classification, and carry out respectively 25 ℃ and 36 ℃ of separation and Culture lawn is extracted to purifying, and by 18 ℃ of cryodrying powdereds of purification thing;
(B) by the vegetable seed dregs of fat, carrier, sucrose, nano controlled-release carrier complexes, sodium nitrophenolate and sodium humate, the mass ratio according to claim 1 requires to send in stirring mixer to be uniformly mixed, and is ground into powder by biotechnology Pengization;
(C) filter residue step (B) obtained is mixed with ostreae testa pulverata, and sends moisture eliminator to and carry out drying, obtains mixing ostreae testa pulverata, and the Temperature Setting of described moisture eliminator is 120～150 ℃, and be 12～17 minutes time of drying;
(D) the mixing ostreae testa pulverata mixing and stirring that powder step (A) obtained and step (C) obtain;
(E) mixed powder after being uniformly mixed is crossed to 38 mesh sieves, then send in fermentation vat and fermented, the temperature in fermentation vat is controlled under 22～29 ℃, ferment 7～10 days, finally the liquid after fermentation is filtered to drying;
Obtain nanometer antiviral aquaculture fertilizer of the present invention.
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| CN201310426803.9A CN103497048B (en) | 2013-09-18 | 2013-09-18 | Antiviral nano aquiculture fertilizer and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105541437A (en) * | 2015-12-29 | 2016-05-04 | 苏州市阳澄湖三家村蟹业有限公司 | Biomass fertilizer for aquaculture |
| CN109180332A (en) * | 2018-09-30 | 2019-01-11 | 广西走山农牧开发有限公司 | The antiviral sustained release water of one kind supports vegetable fertilizer and preparation method thereof |
| CN113383873A (en) * | 2021-06-30 | 2021-09-14 | 烟台开发区天源水产有限公司 | Aquaculture fertilizer and preparation method thereof |
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| CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
| CN101805222A (en) * | 2010-02-25 | 2010-08-18 | 西安久丰有机农业科技研发有限公司 | High-efficiency organic fertilizer combining with medicinal properties and manufacturing method thereof |
| CN102826897A (en) * | 2012-07-31 | 2012-12-19 | 马鞍山科邦生态肥有限公司 | Preparation method of special fertilizer for rice base fertilizer |
| CN103004883A (en) * | 2011-09-21 | 2013-04-03 | 林治 | Aquaculture health preserving bacteriocin |
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2013
- 2013-09-18 CN CN201310426803.9A patent/CN103497048B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
| CN101805222A (en) * | 2010-02-25 | 2010-08-18 | 西安久丰有机农业科技研发有限公司 | High-efficiency organic fertilizer combining with medicinal properties and manufacturing method thereof |
| CN103004883A (en) * | 2011-09-21 | 2013-04-03 | 林治 | Aquaculture health preserving bacteriocin |
| CN102826897A (en) * | 2012-07-31 | 2012-12-19 | 马鞍山科邦生态肥有限公司 | Preparation method of special fertilizer for rice base fertilizer |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105541437A (en) * | 2015-12-29 | 2016-05-04 | 苏州市阳澄湖三家村蟹业有限公司 | Biomass fertilizer for aquaculture |
| CN109180332A (en) * | 2018-09-30 | 2019-01-11 | 广西走山农牧开发有限公司 | The antiviral sustained release water of one kind supports vegetable fertilizer and preparation method thereof |
| CN113383873A (en) * | 2021-06-30 | 2021-09-14 | 烟台开发区天源水产有限公司 | Aquaculture fertilizer and preparation method thereof |
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