The present invention relates to the health food technology field, particularly a kind of health food with anti-oxidation function.
Aging typically refers under normal condition after the biological development maturation, increases with the age, and self hypofunction, homeostasis ability and stress ability decline, the progressively retrogression of structure, function, deathward, irreversible phenomenon.Aging is a kind of biological process of complexity, is zoic common trait.Aging is divided into physiological aging and pathological seaility two classes, and the physiological aging is the retrogression that organism must occur in the growth overall process, and pathological seaility is the aging caused due to various diseases.Most of the elderlys, both often exist simultaneously and interact, can not be well-separated.
Enter 21 century, along with continuing to increase of the aged, aging population has become global social concern, and antidotal research has become the focus of international the world of medicine.
Modern medicine mainly contains free radical theory, tolemerase, gene regulation theory, injury of mitochondria theory etc. to the research of Aging mechanism, mainly contain antioxidant for antidotal medicine, antiaging hormone, the nutrient classes such as protein, nucleic acid, each seed amino acid, MAOI, immunomodulator etc.
Menopausal syndrome is due to ovarian function decline, and the ovarian secretion hormone reduces and the symptom of the neuroendocrine dysequilibrium that causes, and clinical manifestation is paramenia, insomnia, failure of memory and depression, anxiety etc.Ovarian function decline and hormone secretion reduce all and increase with the interior activities of antioxidant enzymes decline of ovary tissue, content of oxygen free radical, luteolysis, the interior mitochondria of ovary and immune variation etc. are related.So far the good method still well do not delayed senility.
In order to solve the problem of above aging, the invention provides and a kind ofly can remove interior free yl, there is certain antioxidation, can improve the health food of climacteric oxidation resistance.
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
Haematococcus pluvialis 50-150 part, grape skin extract 50-150 part, soybean lecithin 10-100 part.
90 parts of haematococcus pluvialis, 90 parts of grape skin extracts, 20 parts of soybean lecithins.
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
Haematococcus pluvialis 50-150 part, grape skin extract 50-150 part, soybean lecithin 10-100 part, safflower 50-150 part, oryzanol 0.5-3 part.
90 parts of haematococcus pluvialis, 90 parts of grape skin extracts, 20 parts of soybean lecithins, 90 parts, safflower, 1.5 parts of oryzanol.
Described health food, make capsule, tablet or granule after raw material is mixed.
Described health food, is characterized in that adding beeswax 10-100 part, linseed oil 150-400 part to make capsule.
Health food of the present invention, can improve SOD in serum, T-AOC and GSH-Px, reduces H
2o
2content, successful, can remove interior free yl, has certain antioxidation, can improve the climacteric oxidation resistance; And formula is simple, be easy to make; Little to the human body side effect.
The specific embodiment
For a better understanding of the present invention, below in conjunction with specific embodiment, further illustrate.
embodiment 1:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
50 parts of haematococcus pluvialis, 150 parts of grape skin extracts, 10 parts of soybean lecithins.
After being mixed, raw material makes capsule, tablet or granule.
Add 150 parts of 100 parts, beeswax and linseed oil to make capsule.
embodiment 2:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
150 parts of haematococcus pluvialis, 50 parts of grape skin extracts, 100 parts of soybean lecithins.
After being mixed, raw material makes capsule, tablet or granule.
Add 400 parts of 10 parts, beeswax and linseed oil to make capsule.
embodiment 3:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
90 parts of haematococcus pluvialis, 90 parts of grape skin extracts, 20 parts of soybean lecithins.
After being mixed, raw material makes capsule, tablet or granule.
Add 280 parts of 20 parts, beeswax and linseed oil to make capsule.
embodiment 4:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
90 parts of haematococcus pluvialis, 90 parts of grape skin extracts, 20 parts of soybean lecithins, 90 parts, safflower, 1.5 parts of oryzanol.
After being mixed, raw material makes capsule, tablet or granule.
Add 280 parts of 20 parts, beeswax and linseed oil to make capsule.
embodiment 5:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
50 parts of haematococcus pluvialis, 150 parts of grape skin extracts, 10 parts of soybean lecithins, 150 parts, safflower, 3 parts of oryzanol.
After being mixed, raw material makes capsule, tablet or granule.
Add 150 parts of 100 parts, beeswax and linseed oil to make capsule.
embodiment 6:
A kind of health food with anti-oxidation function, the raw material weight proportioning is as follows:
150 parts of haematococcus pluvialis, 50 parts of grape skin extracts, 100 parts of soybean lecithins, 50 parts, safflower, 0.5 part of oryzanol.
After being mixed, raw material makes capsule, tablet or granule.
Add 400 parts of 10 parts, beeswax and linseed oil to make capsule.
the compliance test result experiment
1 materials and methods
1.1 animal
Healthy Wistar female rats of 11~12 monthly ages, weight 180~200g,, provided by Shandong University's Experimental Animal Center by 30.
1.2 medicine, reagent and instrument
The capsule prepared in embodiment 1-3, for by our company's self-control, be mixed with the solution for standby of active constituent content 0.5g/mL with distilled water; Isoflavones, Nanning Fresh-life Technology Co., Ltd.'s product.Chloraldurate, space Longhai City, Qingdao algae Co., Ltd product; SOD, T-AOC, H
2o
2with the GSH-Px detection kit, Nanjing is built up bio-engineering research and is provided.The CT14RD table-type high-speed refrigerated centrifuge, sky, Shanghai U.S. biochemical instrument plant engineering Co., Ltd product; The VIS-723N visible spectrophotometer, Beijing Rayleigh Analytical Instrument Co.,Ltd's product; The digital display thermostat water bath, Zhengzhou Tu Fu instrument plant product.
1.3 foundation and the drug intervention of animal grouping, model
42 healthy Wistar female rats are divided into to 7 groups of the blank group (castration group) of sham-operation group, castration, isoflavones group, embodiment 1 Capsules group, embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group, 6 every group at random.Adopt excision bilateral ovaries method to set up rat climacteric model, and make the colpocytology smear and determine that whether model is successful.Sham-operation group and castration group give physiological saline 0.01mL/g weight, and the isoflavones group gives isoflavones 0.4mg/g weight, and the embodiment group gives 5mg solution/g weight.Every day, gavage was 1 time, continuously 15d.
1.4 detection index
In experimentation every 3d to rat Weighing body quality.After drug intervention finishes, 100g/L chloral hydrate anesthesia rat, abdominal aortic blood, 2500r/min, centrifugal 10min, get serum, and-20 ℃ save backup.The strict kit specification of pressing is measured SOD, T-AOC, H in serum
2o
2and GSH-Px.
1.5 statistical method
Adopt the SPSS13.0 statistical analysis software to process.Data with mean (
) ± standard deviation (S) means, carries out one-way analysis of variance or non-parametric test.Take P<0.05 has statistical significance as difference.
2 results
2.1 respectively organize the contrast of rat weight
Indivedual animal deads in experimentation.With the sham-operation group, contrast, respectively organize weight difference not statistically significant (P > 0.05) before modeling and before intervening, after the castration group is intervened 9d, 12d and 15d, difference has statistical significance (P<0.05), and other respectively organize difference not statistically significant (P > 0.05).With the contrast of castration group, each drug intervention group weight difference not statistically significant.With the contrast of sham-operation group, all the other respectively organize weight all growth, and wherein the increase of castration group is the most obvious; With the contrast of castration group, each drug intervention group growth of weight is relatively slow.In Table 1.
Table 1 is respectively organized the contrast of rat weight
Annotate: with the sham-operation group, compare,
*p<0.05.
2.2 respectively organize the rat uterus index contrast
With the contrast of sham-operation group, other respectively organize uterus index decline, and difference has statistical significance (P<0.05).With the contrast of castration group, each drug intervention group uterus index increases, and wherein embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group increase difference statistical significance (P<0.05).With the contrast of isoflavones group, embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group uterus indexes increase, and difference has statistical significance (P<0.01).In Table 2
Table 2 is respectively organized the rat uterus index contrast
Group |
Number of animals |
Uterus index |
The sham-operation group |
6 |
0.231±0.042 |
The castration group |
6 |
0.041±0.009
* |
The isoflavones group |
5 |
0.045±0.006
* |
Embodiment 1 Capsules group |
5 |
0.052±0.003
* |
Embodiment 2 Capsules group |
6 |
0.059±0.007
*#△△ |
Embodiment 3 Capsules group |
6 |
0.061±0.004
*#△△ |
Embodiment 4 Capsules group |
6 |
0.065±0.002
*#△△ |
Annotate: with the sham-operation group, compare,
*p<0.05; With the contrast of castration group,
#p<0.05; With the contrast of isoflavones group,
△ △p<0.01.
2.3 respectively organize Antioxidant Indexes contrast in rat blood serum
With the sham-operation group, contrast, all the other are respectively organized SOD content in serum, T-AOC content and GSH-Px vigor and descend, castration group and isoflavones group SOD, GSH-Px difference have statistical significance (P<0.05 or P<0.01), and each organizes T-AOC content difference statistical significance (P<0.01); Each organizes H in serum
2o
2content obviously increases (P<0.01).With the castration group, contrast, SOD content in each drug intervention group serum, T-AOC content and GSH-Px vigor increase, wherein embodiment 1 Capsules group, embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group SOD, the increase of T-AOC content the most obviously (P<0.01); H in each drug intervention group serum
2o
2content reduces, and difference has statistical significance (P<0.01).With the contrast of isoflavones group, in embodiment 1 Capsules group, embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group serum, SOD content, T-AOC content and GSH-Px vigor increase, and difference has statistical significance (P<0.05 or P<0.01); H in embodiment 1 Capsules group, embodiment 2 Capsules group, embodiment 3 Capsules group and embodiment 4 Capsules group serum
2o
2content obviously reduces (P<0.01).In Table 3
Table 3 is respectively organized rat blood serum SOD, T-AOC, H
2o
2and GSH-Px contrast
Group |
Number of animals |
SOD/(U·mL
-1)
|
T-AOC(U·mL
-1)
|
H
2O
2/( mmol·L
-1)
|
GSH-Px/( U·L
-1)
|
The sham-operation group |
6 |
114.08±20.16 |
10.21±0.91 |
17.42±1.69 |
1610.34±51.04 |
The castration group |
6 |
61.26±23.54
** |
6.85±0.72
** |
66.71±4.07
** |
1279.63±159.31
* |
The isoflavones group |
5 |
78.13±21.42
* |
7.27±0.69
** |
43.24±1.92
**## |
1378.83±28.06
** |
Embodiment 1 Capsules group |
5 |
101.25±16.71
##△ |
8.11±0.62
**##△△ |
32.54±2.18
**##△△ |
1522.44±32.24
△△ |
Embodiment 2 Capsules group |
6 |
104.66±12.47
##△ |
8.74±0.59
**##△△ |
30.27±2.15
**##△△ |
1550.06±21.87
△△ |
Embodiment 3 Capsules group |
6 |
107.24±14.03
##△ |
8.98±0.48
**##△ |
26.76±2.04
**##△△ |
1597.14±20.21
△△ |
Embodiment 3 Capsules group |
6 |
112.48±13.15
##△ |
9.23±0.34
**##△ |
22.25±2.13
**##△△ |
1632.23±20.85
△△ |
Annotate: with the sham-operation group, compare,
*p<0.05,
*p<0.01; With the contrast of castration group,
##p<0.01; With the contrast of isoflavones group,
△p<0.05,
△ △p<0.01.
3 discuss
Menopausal syndrome is due to ovarian function decline, and the ovarian secretion hormone reduces and the symptom of the neuroendocrine dysequilibrium that causes, and clinical manifestation is paramenia, insomnia, failure of memory and depression, anxiety etc.Ovarian function decline and hormone secretion reduce all and increase with the interior activities of antioxidant enzymes decline of ovary tissue, content of oxygen free radical, luteolysis, the interior mitochondria of ovary and immune variation etc. are related.T-AOC is the index of weighing body antioxidant system integration capability, can reflect on the whole the level of antioxidant ability of organism; SOD is free radical and MDA scavenger in cell, can be converted into H to harmful superoxide radical
2o
2; H in cell
2o
2can be transformed into the high activity hydroxy radical, cause the fracture of DNA chain; GSH-Px
Produce the chain reaction of various free radicals in mitochondrial respiratory chain electron transfer process capable of blocking, avoid cell to be subject to the damage of peroxide.
This result of study shows, castration group Weight gain, and uterus index reduces, and with the contrast of sham-operation group, difference has statistical significance, illustrates that ovary and changes of body mass and uterus index have substantial connection, and its mechanism awaits further discussion.In castration group rat blood serum, SOD, T-AOC and GSH-Px reduce, H
2o
2increase; Give after the different pharmaceutical effect SOD, T-AOC and GSH-Px in serum and improved in various degree, H
2o
2content reduces, and wherein embodiment 3 Capsules group effects are the most obvious, and hence one can see that, and health products of the present invention can be removed interior free yl, have certain antioxidation, can improve the hot flushes in rats oxidation resistance.