CN104905321A - Wall-breaking Haematoccoccus Pluvialis powder microcapsules and preparation process thereof - Google Patents
Wall-breaking Haematoccoccus Pluvialis powder microcapsules and preparation process thereof Download PDFInfo
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Abstract
The present invention discloses a wall-breaking Haematoccoccus Pluvialis powder microcapsule preparation process, which comprises: 1, carrying out a wall breaking treatment on Haematoccoccus Pluvialis powder; 2, taking sugar and protein, dissolving in water, adjusting the pH value of the sugar and protein aqueous solution to 6-9, and reacting for 0.5-6 h at a temperature of 95-135 DEG C so as to make the sugar and the protein be subjected to a Maillard reaction, wherein a ratio of the water to the total amount of the sugar and the protein is 1:0.2-0.4, the sugar is any one selected from glucose, lactose and maltose, and the protein is casein or soy protein isolate; and 3, adopting the Maillard reaction product obtained in the step 2 as the wall material and adopting the wall-breaking Haematoccoccus Pluvialis powder obtained in the step 1 as the core material to prepare the wall-breaking Haematoccoccus Pluvialis powder microcapsules. The present invention further discloses the wall-breaking Haematoccoccus Pluvialis powder microcapsules, which have characteristics of high astaxanthin content, good stability and good water solubility so as to increase the application range in the liquid food.
Description
Technical field
The present invention relates to microcapsules technology field, be specifically related to a kind of preparation technology of broken wall haematococcus pluvialis powder capsule, also relate to a kind of broken wall haematococcus pluvialis powder capsule simultaneously.
Background technology
Haematococcus pluvialis (Haematoccoccus pluvialis) is a kind of widely distributed monoplast green alga, akinete can be formed and the multiple carotenoid with physiological function of a large amount of accumulation under adverse environmental factor, mainly comprise astaxanthin, astaxanthin monoesters, astaxanthin diester, lutern, beta carotene, echinenone, angle xanthin etc., wherein 80% is astaxanthin and ester class thereof.Haematococcus pluvialis is the biology that the content astaxanthin of current known occurring in nature existence is the highest, can reach 1.5-4% dry weight.
Astaxanthin makes it have the highest antioxidation activity due to the carbonyl in its end-rings and hydroxyl, its oxidation resistance is 10 times of beta carotene, 60 times of OPC, 550 times of (Miki W. of vitamin E, Biological functions and activities of animal carotenoids.Pure Appl.Chem., 1991, Vol.63, No.1, pp.141-146).In addition, astaxanthin has very strong Tumor suppression to be generated, strengthen immunologic function, resists the multiple physiological functions such as uv damage (Pashkow FJ, Watumull DG, Campbell CL.Astaxanthin:a novel potential treatment for oxidative stress and inflammation in cardiovascular disease.Am J Cardiol.2008May22; 101 (10A): 58D-68D.), be thus widely used in the fields such as food, medicine, cosmetics, health products, aquaculture.Obtain the approval of FDA in the U.S., allowed to enter health-product market as new dietary ingredient.Superpower oxidation resistance and multiple biologically active, and gorgeous redness imparts astaxanthin application prospect and market potential widely, it has been widely used in the fields such as aquatic products, poultry farming, food, health products, cosmetics and pharmaceutical sector.
For the microencapsulation embedding treatment of the Astaxanthin extraction method in the broken wall of haematococcus pluvialis or haematococcus pluvialis or astaxanthin oil, document is had to report separately at present, astaxanthin oil is carried out the extraction that microencapsulation embedding needs to carry out astaxanthin, process is complicated and output and effect all have loss, can not play effect of haematococcus pluvialis completely.The report of microencapsulation process is directly carried out after there is no haematococcus pluvialis broken wall at present.Mainly owing to there is following problem: the cell wall fragments of broken wall haematococcus pluvialis adds microencapsulation intractability; The conventional wall material such as converted starch, Arabic gum embedding weak effect, causes product stability poor, and directly can cause the oxidational losses of astaxanthin oil to the spraying of broken wall haematococcus pluvialis liquid.
Summary of the invention
An object of the present invention is to solve at least the problems referred to above and/or defect, and the advantage will illustrated at least is below provided.
A further object of the invention is to provide a kind of preparation technology of broken wall haematococcus pluvialis powder capsule, the present invention passes through maillard reaction product as wall material, and be combined spray method, the cell wall fragments effectively overcoming broken wall haematococcus pluvialis is difficult to the problem of microencapsulation, directly microencapsulation is carried out to broken wall algae powder, micro-encapsulated astaxanthin is combined with algae dried bean noodles is dry, produce the micro-encapsulated astaxanthin product containing algae powder, decrease product extraction process, the content adopting maillard reaction product to avoid astaxanthin as wall material reduces, it also avoid the oxidational losses of astaxanthin oil.
Another object of the present invention is to provide a kind of broken wall haematococcus pluvialis powder capsule, broken wall haematococcus pluvialis powder capsule of the present invention effectively reduces the surperficial content astaxanthin of microcapsules, mask the fishy smell of astaxanthin oil, there is good stability and water-soluble, content astaxanthin reaches 0.5-1%, and product quality is high.
For this reason, technical scheme provided by the invention is:
A preparation technology for broken wall haematococcus pluvialis powder capsule, comprises the steps:
Step one, broken wall treatment is carried out to pending haematococcus pluvialis powder;
Step 2, with part by weight 1: 0.5 ~ 3 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.2 ~ 0.4, the aqueous solution regulating sugar and protein is afterwards 6-9 to pH, at temperature 95-135 DEG C, react 0.5-6h again, make sugar and protein generation Maillard reaction;
Wherein, described sugar is any one in glucose, lactose or maltose, and described protein is casein or soybean protein isolate; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one prepares broken wall haematococcus pluvialis powder capsule as core.
Preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step 2, the actual conditions that described Maillard reaction occurs is: the part by weight of described sugar and protein is 1: 1, the aqueous solution regulating sugar and protein is 8.5 to pH, then at reaction temperature 105 DEG C, reacts 2.5h.
Preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step 3, utilize spray drying process that described core and wall material are prepared into broken wall haematococcus pluvialis powder capsule, wherein, during spraying dry, EAT is 150-200 DEG C, and leaving air temp is 60-100 DEG C.
Preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step 3, utilize before described core and wall material be prepared into broken wall haematococcus pluvialis powder capsule by spray drying process, also comprise the steps:
(3.1) core wall material mixing: be 1: 2.5 ~ 4 get the wall material that obtains in the core and step 2 obtained in step one and mix by weight proportion; And,
(3.2) homogeneous: under high-pressure homogeneous pressure 30-50MP, carries out homogeneous 2 ~ 3 times to the mixed solution of the core obtained in step (3.1) and wall material.
Preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step one, any one method in following methods is adopted to carry out broken wall treatment to pending haematococcus pluvialis powder:
(1) high pressure homogenizer mechanical breaking-wall method: in temperature 4 DEG C, carry out homogeneous under pressure 40-150MP;
(2) ball mill mechanical breaking-wall method: process 1-2h under rotating speed 400-600rpm; Or,
(3) first enzyme process haematococcus pluvialis powder 1h under temperature 30 ~ 60 DEG C of pretreatment is adopted, the enzyme adopted is that total amount accounts for the pectase of described haematococcus pluvialis powder weight 0.5 ~ 2 ‰, cellulase and protease, add HCl more afterwards or citric acid carries out heating 30-60min, or carry out ball-milling treatment 60-120min.
Even more preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step one, in described method (3), the percent by volume of described HCl is 1% ~ 5%, and the mass percent of described citric acid is 1% ~ 5%.
Even more preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step one, carry out adding the antioxidant accounting for described haematococcus pluvialis powder weight 0.1-2% in the forward direction haematococcus pluvialis powder of broken wall treatment, wherein said antioxidant is selected from one or more in VC, VE or BHT.
Even more preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, EAT during described spraying dry is 180 DEG C, and leaving air temp is 80 DEG C.
Even more preferably, in the preparation technology of described broken wall haematococcus pluvialis powder capsule, in described step (3.1), the part by weight of described core and wall material is 1: 2.5; In described step (3.2), described high-pressure homogeneous pressure is under 40MP, and the number of times of homogeneous is 2 times.
A kind of broken wall haematococcus pluvialis powder capsule, described broken wall haematococcus pluvialis powder capsule is obtained by arbitrary described method.
The present invention at least comprises following beneficial effect:
The present invention utilizes Maillard reaction thing to prepare broken wall haematococcus pluvialis microcapsules for main wall material, obtained broken wall haematococcus pluvialis microcapsules are red powder, according to the drying process with atomizing that the present invention optimizes, microcapsule embedded rate and product rate all reach more than 90%, content astaxanthin reaches 0.5-1%, there is good stability and water-soluble, increase its range of application in liquid food.
The present invention with the addition of antioxidant in technology for broken wall, uses, serve Double-protection effect to astaxanthin in conjunction with the anti-oxidant wall material of Maillard reaction thing.
Adopt the technological parameter of wall material of the present invention and optimization, as mixing, homogeneous etc., effectively reduce the surperficial content astaxanthin of microcapsules, mask the fishy smell of astaxanthin oil, improve the quality of products.
Present invention process is simple, and product yield is high, and production cost is low.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Accompanying drawing explanation
Fig. 1 is the Electronic Speculum figure of the non-broken wall haematococcus pluvialis cell that in one of them embodiment of the present invention, electron microscope observation arrives;
Fig. 2 is the Electronic Speculum figure of the haematococcus pluvialis cell after the broken wall that in one of them embodiment of the present invention, electron microscope observation arrives;
Fig. 3 is the electron-microscope scanning figure of the broken wall haematococcus pluvialis powder of microencapsulation embedding in one of them embodiment of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to description word to make those skilled in the art.
Should be appreciated that used hereinly such as " to have ", other element one or more do not allotted in " comprising " and " comprising " term or the existence of its combination or interpolation.
The invention provides a kind of preparation technology of broken wall haematococcus pluvialis powder capsule, comprise the steps:
Step one, broken wall treatment is carried out to pending haematococcus pluvialis powder;
Step 2, with part by weight 1: 0.5 ~ 3 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.2 ~ 0.4, the aqueous solution regulating sugar and protein is afterwards 6-9 to pH, at temperature 95-135 DEG C, react 0.5-6h again, make sugar and protein generation Maillard reaction;
Wherein, described sugar is any one in glucose, lactose or maltose, and described protein is casein or soybean protein isolate; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one prepares broken wall haematococcus pluvialis powder capsule as core.
Maillard reaction product (Maillard Reaction Product, MRPs) has certain antioxygenic property, and wherein the oxidation resistance of Cucumber can compare favourably with conventional antioxidant in food; The reaction of different substrate can form the flavor substance of different characteristic, gives the special favor that product is new.Meanwhile, Maillard reaction thing has excellent emulsification and filming performance as wall material.Microencapsulation can reduce astaxanthin light, oxygen, water mitigation effectively, reduces oxidation; Prevent the volatilization of core in storage process; The release of protection and control core; Change the physicochemical property such as color, shape, density, dispersive property of core; The special fishy taste reducing or cover in astaxanthin finish; Improve and the Combination of other material and mobility thereof etc.Therefore as wall material, microencapsulation embedding is carried out to astaxanthin with water-soluble maillard reaction product, effectively can improve stability, expand its range of application.Broken wall haematococcus pluvialis microcapsules prepared by the present invention are red powder.According to method of the present invention, these microcapsules have good stability and water-soluble, add its range of application in liquid food.Present invention process is simple, and product yield is high, and production cost is low.
As preferably, in described step 2, the actual conditions that described Maillard reaction occurs is: the part by weight of described sugar and protein is 1: 1, and the aqueous solution regulating sugar and protein is 8.5 to pH, then at reaction temperature 105 DEG C, reacts 2.5h.
As preferably, in described step 3, utilize spray drying process that described core and wall material are prepared into broken wall haematococcus pluvialis powder capsule, wherein, during spraying dry, EAT is 150-200 DEG C, and leaving air temp is 60-100 DEG C.Spray drying process prepares emulsion that microcapsules are formed primarily of core and wall material in the atomization drying of drying tower.In spray process, although the temperature of hot-air is very high, due to water rapid evaporation from wall material, thus ensure that core temperature is lower than 100 DEG C, is therefore specially adapted to the drying of the stronger material of thermal sensitivity.The present invention using maillard reaction product as wall material and spray drying process coupling, the microcapsule embedded rate prepared according to the present invention is made all to reach more than 90% with product rate, content astaxanthin reaches 0.5-1%; get the broken wall haematococcus pluvialis powder capsule 1g that the present invention obtains and be placed in 10 ~ 20mL water; stir 2 ~ 5min; it can be uniformly dissolved very soon, obtains broken wall haematococcus pluvialis powder microcapsule solution.Namely this broken wall haematococcus pluvialis powder capsule has good stability and water-soluble, adds its range of application in liquid food.
As preferably, EAT during described spraying dry is 180 DEG C, and leaving air temp is 80 DEG C.
As preferably, in described step 3, utilize before described core and wall material be prepared into broken wall haematococcus pluvialis powder capsule by spray drying process, also comprise the steps:
(3.1) core wall material mixing: be 1: 2.5 ~ 4 get the wall material obtained in the core and step 2 obtained in step one by weight proportion, total solid concentration is 15-25%, abundant mix and blend 5-30min, makes it mixing; This total solid comprise mix albumen, polysaccharide and reactant and haematococcus pluvialis etc.And (3.2) homogeneous: under high-pressure homogeneous pressure 30-50MP, carries out homogeneous 2 ~ 3 times to the mixed solution of the core obtained in step (3.1) and wall material.Enter spray drying step more afterwards.Through above step, effectively can reduce the surperficial content astaxanthin of microcapsules, mask the fishy smell of astaxanthin oil, improve the quality of products.
As preferably, in described step (3.1), the part by weight of described core and wall material is 1: 2.5, total solid concentration 20%, fully stirs 10min and makes it mixing; In described step (3.2), described high-pressure homogeneous pressure is under 40MP, and the number of times of homogeneous is 2 times.
As preferably, in described step one, any one method in following methods is adopted to carry out broken wall treatment to pending haematococcus pluvialis powder:
(1) high pressure homogenizer mechanical breaking-wall method: in temperature 4 DEG C, carry out homogeneous under pressure 40-150MP;
(2) ball mill mechanical breaking-wall method: process 1-2h under rotating speed 400-600rpm; Or,
(3) first enzyme process haematococcus pluvialis powder 1h under temperature 30 ~ 60 DEG C of pretreatment is adopted, the enzyme adopted is that total amount accounts for the pectase of described haematococcus pluvialis powder weight 0.5 ~ 2 ‰, cellulase and protease, add HCl more afterwards or citric acid carries out heating 30-60min, or carry out ball-milling treatment 60-120min.As shown in Figure 1, Figure 2 and Figure 3, after above method broken wall, the sporoderm-broken rate of haematococcus pluvialis powder can reach more than 90%, in general, namely can be used for making microcapsules as long as sporoderm-broken rate will reach 80-100%.The evaluation of sporoderm-broken rate: adopt planktonic organism tally counted under microscope,
As preferably, in described step one, in described method (3), the percent by volume of described HCl is 1% ~ 5%, and the mass percent of described citric acid is 1% ~ 5%.
As preferably, in described step one, carry out adding the antioxidant accounting for described haematococcus pluvialis powder weight 0.1-2% in the forward direction haematococcus pluvialis powder of broken wall treatment, wherein said antioxidant is selected from one or more in VC, VE or BHT.Antioxidant is added in technology for broken wall, and Maillard reaction thing anti-oxidant wall material conbined usage, Double-protection effect is served to astaxanthin.
A kind of broken wall haematococcus pluvialis powder capsule, described broken wall haematococcus pluvialis powder capsule is obtained by arbitrary described method.
Embodiment 1
Broken wall haematococcus pluvialis powder microcapsule preparation process:
Prepare 8% haematococcus pluvialis amidin, add algae dried bean noodles and weigh the antioxidant of 0.1% as vitamin C-sodium, adopt high pressure homogenizer machinery broken wall law, the broken wall algae powder solution of obtained sporoderm-broken rate more than 90% is as core;
Prepare maillard reaction product wall material, sugar and protein ratio are 1: 1, pH8.5, reaction temperature 110 DEG C, and the reaction time is 2.5h;
Core wall material is mixed abundant mix and blend 5-10min; High-pressure homogeneous, homogenization pressure 30-50MP, homogeneous 2 times; Finally carry out spraying dry.
Before described wall material mixes with core, cross 100 mesh sieve thin,tough silk; The quality proportioning of described core and wall material is 1: 3, and total solid concentration is 20%; Described spraying dry EAT is 180 DEG C, and leaving air temp is 80 DEG C.
Embodiment 2
Broken wall haematococcus pluvialis powder microcapsule preparation process:
Prepare 10% haematococcus pluvialis amidin, add the heavy antioxidant of algae dried bean noodles as vitamin E, adopt ball mill machinery broken wall law, the obtained broken wall algae powder solution of sporoderm-broken rate more than 90%; Prepare maillard reaction product wall material, sugar and protein ratio are 1: 0.8, pH8.0, reaction temperature 110 DEG C, and the reaction time is 3.5h; Core wall material is mixed abundant mix and blend 5-10min; High-pressure homogeneous, homogenization pressure 30-50MP, homogeneous 2 times; Finally carry out spraying dry.
Before described wall material mixes with core, cross 100 mesh sieve thin,tough silk; The quality proportioning of described core and wall material is 1: 4, and total solid concentration is 18%; Described spraying dry EAT is 180 DEG C, and leaving air temp is 80 DEG C.
Embodiment 3
Broken wall haematococcus pluvialis powder microcapsule preparation process, comprises the steps:
Step one, by water-soluble for the haematococcus pluvialis powder formation aqueous solution, add wherein afterwards and account for antioxidant VC and VE of described haematococcus pluvialis powder weight 0.1%.Utilize high pressure homogenizer in temperature 4 DEG C afterwards, under pressure 40MP, carry out homogeneous, with mechanical breaking-wall method, obtain broken wall haematococcus pluvialis powder as core.
Step 2, with part by weight 1: 0.5 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.2, the aqueous solution regulating sugar and protein is afterwards 6 to pH, 6h is reacted again at temperature 95 DEG C, make sugar and protein generation Maillard reaction, and using product as wall material;
Wherein, described sugar is glucose, and described protein is casein; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one, as core, prepares broken wall haematococcus pluvialis powder capsule in accordance with the following steps:
(3.1) core wall material mixing: be get the wall material that in the core and step 2 that in step one obtain obtain and mix at 1: 3 by weight proportion; And,
(3.2) homogeneous: under high-pressure homogeneous pressure 30MP, carries out homogeneous 3 times to the mixed solution of the core obtained in step (3.1) and wall material.
(3.3) the described core utilizing spray drying process to be crossed by homogeneous and the mixed solution of wall material are prepared into broken wall haematococcus pluvialis powder capsule, and wherein, during spraying dry, EAT is 150 DEG C, and leaving air temp is 60 DEG C.
Embodiment 4
Broken wall haematococcus pluvialis powder microcapsule preparation process, comprises the steps:
Step one, by water-soluble for the haematococcus pluvialis powder formation aqueous solution, add wherein afterwards and account for the antioxidant VC of described haematococcus pluvialis powder weight 1%.First enzyme process haematococcus pluvialis powder 1h under temperature 30 ~ 60 DEG C of pretreatment is adopted afterwards, the enzyme adopted is that total amount accounts for the pectase of described haematococcus pluvialis powder weight 0.5 ~ 2 ‰, cellulase and protease, add HCl more afterwards or citric acid carries out heating 30-60min, or carry out ball-milling treatment 60-120min.The percent by volume of described HCl is 1% ~ 5%, and the mass percent of described citric acid is 1% ~ 5%.With broken wall, obtain broken wall haematococcus pluvialis powder as core.
Step 2, with part by weight 1: 1 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.3, the aqueous solution regulating sugar and protein is afterwards 8.5 to pH, 2.5h is reacted again at temperature 105 DEG C, make sugar and protein generation Maillard reaction, and using product as wall material;
Wherein, described sugar is maltose, and described protein is casein; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one, as core, prepares broken wall haematococcus pluvialis powder capsule in accordance with the following steps:
(3.1) core wall material mixing: be get the wall material that in the core and step 2 that in step one obtain obtain and mix at 1: 2.5 by weight proportion; And,
(3.2) homogeneous: under high-pressure homogeneous pressure 40MP, carries out homogeneous 2 times to the mixed solution of the core obtained in step (3.1) and wall material.
(3.3) the described core utilizing spray drying process to be crossed by homogeneous and the mixed solution of wall material are prepared into broken wall haematococcus pluvialis powder capsule, and wherein, during spraying dry, EAT is 180 DEG C, and leaving air temp is 80 DEG C.
Embodiment 5
Broken wall haematococcus pluvialis powder microcapsule preparation process, comprises the steps:
Step one, by water-soluble for the haematococcus pluvialis powder formation aqueous solution, add wherein afterwards and account for the antioxidant BHT of described haematococcus pluvialis powder weight 2%, wherein said antioxidant is selected from one or more in VC, VE or BHT.Utilize ball mill mechanical breaking-wall method afterwards: under rotating speed 400-600rpm, process 1-2h with mechanical breaking-wall method, obtain broken wall haematococcus pluvialis powder as core.
Step 2, with part by weight 1: 3 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.4, the aqueous solution regulating sugar and protein is afterwards 9 to pH, 0.5h is reacted again at temperature 135 DEG C, make sugar and protein generation Maillard reaction, and using product as wall material;
Wherein, described sugar is lactose, and described protein is soybean protein isolate; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one, as core, prepares broken wall haematococcus pluvialis powder capsule in accordance with the following steps:
(3.1) core wall material mixing: be get the wall material that in the core and step 2 that in step one obtain obtain and mix at 1: 4 by weight proportion; And,
(3.2) homogeneous: under high-pressure homogeneous pressure 50MP, carries out homogeneous 2 times to the mixed solution of the core obtained in step (3.1) and wall material.
(3.3) the described core utilizing spray drying process to be crossed by homogeneous and the mixed solution of wall material are prepared into broken wall haematococcus pluvialis powder capsule, and wherein, during spraying dry, EAT is 200 DEG C, and leaving air temp is 100 DEG C.
The present invention passes through maillard reaction product as wall material, and be combined spray method, the cell wall fragments effectively overcoming broken wall haematococcus pluvialis is difficult to the problem of microencapsulation, directly microencapsulation is carried out to broken wall algae powder, micro-encapsulated astaxanthin is combined with algae dried bean noodles is dry, produce the micro-encapsulated astaxanthin product containing algae powder, decrease product extraction process, the content adopting maillard reaction product to avoid astaxanthin as wall material reduces, and it also avoid the oxidational losses of astaxanthin oil.This process set sealing process of microencapsulation and the antioxidation of maillard reaction product itself, have the effect of stronger protection astaxanthin.Make the microcapsule embedded rate prepared according to the present invention and produce rate all to reach more than 90%, content astaxanthin reaches 0.5-1%, has good stability and water-soluble, adds its range of application in liquid food.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (10)
1. a preparation technology for broken wall haematococcus pluvialis powder capsule, is characterized in that, comprises the steps:
Step one, broken wall treatment is carried out to pending haematococcus pluvialis powder;
Step 2, with part by weight 1: 0.5 ~ 3 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.2 ~ 0.4, the aqueous solution regulating sugar and protein is afterwards 6-9 to pH, at temperature 95-135 DEG C, react 0.5-6h again, make sugar and protein generation Maillard reaction;
Wherein, described sugar is any one in glucose, lactose or maltose, and described protein is casein or soybean protein isolate; And,
The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one prepares broken wall haematococcus pluvialis powder capsule as core.
2. broken wall haematococcus pluvialis powder microcapsule preparation process as claimed in claim 1, it is characterized in that, in described step 2, the actual conditions that described Maillard reaction occurs is: the part by weight of described sugar and protein is 1: 1, the aqueous solution regulating sugar and protein is 8.5 to pH, then at reaction temperature 105 DEG C, reacts 2.5h.
3. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 1, it is characterized in that, in described step 3, utilize spray drying process that described core and wall material are prepared into broken wall haematococcus pluvialis powder capsule, wherein, during spraying dry, EAT is 150-200 DEG C, and leaving air temp is 60-100 DEG C.
4. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 3, is characterized in that, in described step 3, utilizes before described core and wall material be prepared into broken wall haematococcus pluvialis powder capsule by spray drying process, also comprises the steps:
(3.1) core wall material mixing: be 1: 2.5 ~ 4 get the wall material that obtains in the core and step 2 obtained in step one and mix by weight proportion; And,
(3.2) homogeneous: under high-pressure homogeneous pressure 30-50MP, carries out homogeneous 2 ~ 3 times to the mixed solution of the core obtained in step (3.1) and wall material.
5. the preparation technology of the broken wall haematococcus pluvialis powder capsule as described in as arbitrary in Claims 1-4, is characterized in that, in described step one, adopts any one method in following methods to carry out broken wall treatment to pending haematococcus pluvialis powder:
(1) high pressure homogenizer mechanical breaking-wall method: in temperature 4 DEG C, carry out homogeneous under pressure 40-150MP;
(2) ball mill mechanical breaking-wall method: process 1-2h under rotating speed 400-600rpm; Or,
(3) first enzyme process haematococcus pluvialis powder 1h under temperature 30 ~ 60 DEG C of pretreatment is adopted, the enzyme adopted is that total amount accounts for the pectase of described haematococcus pluvialis powder weight 0.5 ~ 2 ‰, cellulase and protease, add HCl more afterwards or citric acid carries out heating 30-60min, or carry out ball-milling treatment 60-120min.
6. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 5, it is characterized in that, in described step one, in described method (3), the percent by volume of described HCl is 1% ~ 5%, and the mass percent of described citric acid is 1% ~ 5%.
7. broken wall haematococcus pluvialis powder microcapsule preparation process as claimed in claim 5, it is characterized in that, in described step one, carry out adding the antioxidant accounting for described haematococcus pluvialis powder weight 0.1-2% in the forward direction haematococcus pluvialis powder of broken wall treatment, wherein said antioxidant is selected from one or more in VC, VE or BHT.
8. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 3, it is characterized in that, EAT during described spraying dry is 180 DEG C, and leaving air temp is 80 DEG C.
9. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 4, it is characterized in that, in described step (3.1), the part by weight of described core and wall material is 1: 2.5; In described step (3.2), described high-pressure homogeneous pressure is under 40MP, and the number of times of homogeneous is 2 times.
10. a broken wall haematococcus pluvialis powder capsule, is characterized in that, described broken wall haematococcus pluvialis powder capsule is obtained by the method as described in as arbitrary in claim 1 to 9.
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| CN106858357A (en) * | 2017-03-11 | 2017-06-20 | 北京中科卓尔生物科技有限公司 | Haematococcus pluvialis natto fine powder and preparation method thereof |
| CN107048421A (en) * | 2017-03-28 | 2017-08-18 | 常州大学 | A kind of preparation method of anti-oxidant microcapsule wall material |
| CN107582576A (en) * | 2017-10-11 | 2018-01-16 | 杭州鑫伟低碳技术研发有限公司 | A kind of composition with biological inflammation-diminishing function and preparation method thereof |
| CN108624643A (en) * | 2018-04-02 | 2018-10-09 | 中国科学院青岛生物能源与过程研究所 | The method for preparing high added value oligopeptides as raw material using micro- quasi- ball algae-residue |
| CN109169967A (en) * | 2018-09-17 | 2019-01-11 | 东北农业大学 | A kind of preparation method of microencapsulation powdered oil |
| CN110123805A (en) * | 2019-05-27 | 2019-08-16 | 上海交通大学 | A kind of preparation method of self assembly procyanidine nano-complex |
| CN112369534A (en) * | 2020-11-27 | 2021-02-19 | 潍坊加易加生物科技有限公司 | Feed additive for preventing early death of prawns and preparation method and application thereof |
| CN112655952A (en) * | 2020-12-17 | 2021-04-16 | 日照职业技术学院 | Astaxanthin algal oil high internal phase emulsion and preparation method thereof |
| CN112890133A (en) * | 2021-03-24 | 2021-06-04 | 康道生物（南通）有限公司 | Preparation process of haematococcus pluvialis capsules |
| CN113081869A (en) * | 2021-04-16 | 2021-07-09 | 云南爱尔康生物技术有限公司 | Haematococcus pluvialis astaxanthin microcapsule and preparation method thereof |
| WO2022021612A1 (en) * | 2020-07-27 | 2022-02-03 | 中国海洋大学 | Method for preparing water-soluble astaxanthin, and astaxanthin aqueous solution prepared using said method |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106722434A (en) * | 2015-11-19 | 2017-05-31 | 徐州统食品工业有限公司 | A kind of anti-oxidation functional food |
| CN106858357A (en) * | 2017-03-11 | 2017-06-20 | 北京中科卓尔生物科技有限公司 | Haematococcus pluvialis natto fine powder and preparation method thereof |
| CN107048421A (en) * | 2017-03-28 | 2017-08-18 | 常州大学 | A kind of preparation method of anti-oxidant microcapsule wall material |
| CN107582576A (en) * | 2017-10-11 | 2018-01-16 | 杭州鑫伟低碳技术研发有限公司 | A kind of composition with biological inflammation-diminishing function and preparation method thereof |
| CN108624643B (en) * | 2018-04-02 | 2020-11-24 | 中国科学院青岛生物能源与过程研究所 | Method for preparing high value-added oligopeptide by taking nannochloropsis oculata residues as raw materials |
| CN108624643A (en) * | 2018-04-02 | 2018-10-09 | 中国科学院青岛生物能源与过程研究所 | The method for preparing high added value oligopeptides as raw material using micro- quasi- ball algae-residue |
| CN109169967A (en) * | 2018-09-17 | 2019-01-11 | 东北农业大学 | A kind of preparation method of microencapsulation powdered oil |
| CN110123805A (en) * | 2019-05-27 | 2019-08-16 | 上海交通大学 | A kind of preparation method of self assembly procyanidine nano-complex |
| CN110123805B (en) * | 2019-05-27 | 2021-09-28 | 上海交通大学 | Preparation method of self-assembled procyanidine nano-composite |
| WO2022021612A1 (en) * | 2020-07-27 | 2022-02-03 | 中国海洋大学 | Method for preparing water-soluble astaxanthin, and astaxanthin aqueous solution prepared using said method |
| CN112369534A (en) * | 2020-11-27 | 2021-02-19 | 潍坊加易加生物科技有限公司 | Feed additive for preventing early death of prawns and preparation method and application thereof |
| CN112655952A (en) * | 2020-12-17 | 2021-04-16 | 日照职业技术学院 | Astaxanthin algal oil high internal phase emulsion and preparation method thereof |
| CN112890133A (en) * | 2021-03-24 | 2021-06-04 | 康道生物（南通）有限公司 | Preparation process of haematococcus pluvialis capsules |
| CN113081869A (en) * | 2021-04-16 | 2021-07-09 | 云南爱尔康生物技术有限公司 | Haematococcus pluvialis astaxanthin microcapsule and preparation method thereof |
| CN113081869B (en) * | 2021-04-16 | 2022-06-03 | 云南爱尔康生物技术有限公司 | Haematococcus pluvialis astaxanthin microcapsule and preparation method thereof |
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