CN110123805A - A kind of preparation method of self assembly procyanidine nano-complex - Google Patents
A kind of preparation method of self assembly procyanidine nano-complex Download PDFInfo
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- CN110123805A CN110123805A CN201910445652.9A CN201910445652A CN110123805A CN 110123805 A CN110123805 A CN 110123805A CN 201910445652 A CN201910445652 A CN 201910445652A CN 110123805 A CN110123805 A CN 110123805A
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- procyanidine
- casein
- maltodextrin
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- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 238000001338 self-assembly Methods 0.000 title claims abstract description 26
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 55
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 55
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 55
- 239000005018 casein Substances 0.000 claims abstract description 34
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 34
- 235000021240 caseins Nutrition 0.000 claims abstract description 34
- 239000000243 solution Substances 0.000 claims abstract description 18
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 238000012545 processing Methods 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 9
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000011888 foil Substances 0.000 claims description 3
- 230000000873 masking effect Effects 0.000 claims description 3
- 230000036571 hydration Effects 0.000 claims description 2
- 238000006703 hydration reaction Methods 0.000 claims description 2
- 229920001353 Dextrin Polymers 0.000 claims 1
- 239000004375 Dextrin Substances 0.000 claims 1
- 241000209140 Triticum Species 0.000 claims 1
- 235000021307 Triticum Nutrition 0.000 claims 1
- 235000019425 dextrin Nutrition 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 6
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229920001222 biopolymer Polymers 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 108010005094 Advanced Glycation End Products Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 238000012695 Interfacial polymerization Methods 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000879 anti-atherosclerotic effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007720 emulsion polymerization reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004676 glycans Polymers 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002114 nanocomposite Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- -1 polyphenol compound Chemical class 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004845 protein aggregation Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
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Abstract
The invention discloses a kind of preparation methods of self assembly procyanidine nano-complex, are related to food processing technology field, and step includes, step 1: preparing casein and maltodextrin mixture；Step 2: preparing casein-maltodextrin graft；Step 3: preparation procyanidine solution and casein-maltodextrin mixed solution；Step 4: preparation procyanidine nano-complex.Those skilled in the art be dedicated to developing it is a kind of do not add organic solvent, it is easy to operate, highly-safe, stability is good, the preparation method of edible procyanidine nano-complex with good biocompatibility and biodegradability.
Description
Technical field
The present invention relates to food processing technology field more particularly to a kind of preparations of self assembly procyanidine nano-complex
Method.
Background technique
Procyanidine is the natural polyphenol compound that is polymerized by C-C key using flavan-3-alcohol as structural unit.From
In right boundary, the abundance of procyanidine is widely distributed, such as grape pip, apple, peanut skin.Research has shown that these are food-borne
Procyanidine is not only easy to be absorbed by people, and has extremely strong antioxidant activity and free radical scavenging ability.It is protected in medicine
Strong field, procyanidine are proved to can be used for prevention and cure of cardiovascular disease, lowering blood pressure and blood fat, anti-atherosclerosis etc..But
It is that procyanidine has some limitations in the application.Phenolic hydroxyl structure in procyanidine makes it in the natural environment
It is easily oxidized, also, procyanidine is very sensitive to environmental conditions such as light, heat, oxygen, in addition, this water solubility of procyanidine
Molecule is not easy in vivo through cell membrane, and bioavilability is low, half-life short.These combined factors limit procyanidine
Application.
Nano-complex has targeting and slow release effect due to its distinctive small-size effect and quantum effect.Nanometer
The preparation method of compound mainly includes emulsion polymerization, interfacial polymerization, self-assembly method etc..Wherein, self assembly is embedding life
The effective way of object reactive compound is defined as basic structural unit, such as molecule, nano material, makees in hydrogen bond, hydrophobe
With under, non-covalent bond effects such as Van der Waals force, electrostatic force, the process of ordered structure or form, preparation process letter are spontaneously formed
It is single, it is easy to operate, it does not need to can produce stable nano-sized particles using crosslinking agent.Protein-PS is by being self-assembly of
Graft compared with their structural unit, there is better amphipathic and surface-active action, in addition its good biology
Compatibility and biodegradability, so that it is played in the embedding research of bioactive substance unique advantage.
Therefore, those skilled in the art be dedicated to developing it is a kind of do not add organic solvent, it is easy to operate, highly-safe,
The preparation side of good, with good biocompatibility and biodegradability the edible procyanidine nano-complex of stability
Method.
Summary of the invention
In view of the above drawbacks of the prior art, the technical problem to be solved by the present invention is to procyanidine to light, heat, oxygen
The environmental conditions such as gas are very sensitive, and are not easy in vivo through cell membrane, and bioavilability is low, half-life short.
To achieve the above object, the present invention provides the preparation methods of self assembly procyanidine nano-complex, including with
Lower step:
Step 1: preparing casein and maltodextrin mixture
Casein and maltodextrin are added in phosphate buffer, is mixed with magnetic stirrer, obtains casein
With the phosphate solution of maltodextrin, after being hydrated at room temperature, freeze-drying obtains casein and maltodextrin lyophilized products, standby
With；
Step 2: preparing casein-maltodextrin graft
Sieve is crossed after the casein and maltodextrin lyophilized products are pulverized, and is sealed, is placed in closed with masking foil with holes
Self assembly and Maillard reaction are carried out in container, are obtained casein-maltodextrin graft, are deposited in spare at 4 DEG C；
Step 3: preparation procyanidine solution and casein-maltodextrin mixed solution
It weighs procyanidine to be dissolved in deionized water, magnetic agitation obtains procyanidine solution uniformly to being completely dissolved；
It weighs the casein-maltodextrin graft to be dissolved in phosphate buffer, it is mixed to obtain casein-maltodextrin graft
Close solution；
Step 4: preparation procyanidine nano-complex
The procyanidine solution is poured slowly into the casein-maltodextrin mixed solution, at normal temperature magnetic force
It stirs evenly, then carries out precooling processing, then be freeze-dried, finally obtain casein-maltodextrin procyanidine and receive
Rice compound.
Further, in the step 1, the time being stirred is 1h, time of the hydration be for 24 hours, it is described
The time of freeze-drying is 48h.
Further, in the step 1, the mass ratio of the casein and the maltodextrin is 1:2.
Further, in the step 1, the concentration of the phosphate buffer is 0.2M, and pH is 6.0~8.0.
Further, in the step 1, in the phosphate solution of the casein and maltodextrin, the junket egg is adjusted
White concentration is to 0.5%~1% (w/v).
Further, in the step 2, the sieve mesh number is 80 mesh.
Further, in the step 2, the condition of the self assembly and Maillard reaction is relative humidity 79%, temperature
60 DEG C, 15~25h of time.
Further, in the step 3, control the casein-maltodextrin graft mixed liquid concentration be 1%~
2.5% (w/v).
Further, in the step 3, the weighed procyanidine and the casein-maltodextrin graft
Mass ratio is 2:5.
Further, which is characterized in that in the step 4, the temperature of precooling processing is -18 DEG C, and the time is
24h。
The present invention, which provides a kind of self assembly procyanidine nano-complex preparation method, at least has technology beneficial below
Effect:
1, the casein that uses of the present invention is one of milk extensibility albumen, can balance high net charge and low interior dredge
It is aqueous, it is strong with the binding force of polyphenol.
2, the process that casein of the present invention and maltodextrin be self-assembly of amphipathic biopolymer is simple and quick,
And can produce stable nano-sized particles, products obtained therefrom has good amphipathic, surface-active, biocompatibility and life
Biodegradable, and albumen is significantly improved in the application because of the bad bring limitation of dissolubility
3, in preparation method of the present invention, casein, maltodextrin can spontaneously pass through non-covalent bond between procyanidine
Interaction is combined, easy to operate, not high to equipment requirement, is suitble to industrial mass production.It is not related in technical process
The use of organic solvent, safety is higher, is suitble to the application of food, health, field of medicaments.
It is described further below with reference to technical effect of the attached drawing to design of the invention, specific structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Detailed description of the invention
Fig. 1 is junket egg prepared by a kind of self-assembled nanometer compound preparation method of a preferred embodiment of the present invention
The scanning electron microscopic picture of white-maltodextrin procyanidine nano-complex.
Specific embodiment
A preferred embodiment of the present invention is introduced below with reference to Figure of description, keeps its technology contents more clear and just
In understanding.The present invention can be emerged from by many various forms of embodiments, and protection scope of the present invention not only limits
The embodiment that Yu Wenzhong is mentioned.
Self assembly procyanidine nano-complex in the present embodiment the preparation method is as follows:
Step 1: preparing casein and maltodextrin mixture
The casein and maltodextrin that mass ratio is 1:2 are weighed, dissolves junket egg with the phosphate buffer of 0.2M pH 7.0
White and maltodextrin, adjusting casein concentration to 1% (w/v), and 1h is mixed with magnetic stirrer, it forms it into uniformly molten
Liquid.It is hydrated for 24 hours at 20 DEG C of room temperature, is then freeze-dried 48h, obtain casein and maltodextrin lyophilized products.
Step 2: preparing casein-maltodextrin graft
Casein and maltodextrin lyophilized products are pulverized, and cross 80 meshes, is sealed with masking foil with holes, in relative humidity
79%, it under conditions of temperature is 60 DEG C, is placed in closed container and reacts 15h, obtain self assembly and maillard reaction product junket egg
White-maltodextrin graft is deposited in spare at 4 DEG C.
Step 3: preparation procyanidine solution and casein-maltodextrin mixed solution
Weigh with casein-maltodextrin graft mass ratio be 2:5 procyanidine, by procyanidine be dissolved in from
It in sub- water, stirs evenly, making it, all dissolution obtains procyanidine solution.Casein-maltodextrin graft is dissolved in dense
Degree is 0.2M, and pH value obtains casein-maltodextrin and connect in 7.0 phosphate buffer, to adjust concentration to 2.5% (w/v)
Branch object mixed solution.
Step 4: preparation procyanidine nano-complex
Procyanidine solution is poured slowly into casein-maltodextrin graft mixed solution, control procyanidine with
Casein-maltodextrin graft mass ratio is 2:5, is stirred evenly at normal temperature, at -18 DEG C after precooling processing for 24 hours
It is freeze-dried, finally obtains casein-maltodextrin procyanidine nano-complex (as shown in Figure 1).
Reference examples
Step 1: weighing a certain amount of casein, be 0.2M with concentration, the phosphate buffer that pH value is 7.0 dissolves junket egg
It is white, adjusting casein concentration to 1% (w/v), and 1h is mixed with magnetic stirrer, form it into uniform casein phosphoric acid
Salting liquid.
Step 2: a certain amount of procyanidine being dissolved in deionized water, is stirred evenly, making it, all dissolution obtains former flower
Green element solution.
Step 3: procyanidine solution being poured slowly into casein phosphate solution, procyanidine and casein are controlled
Mass ratio is 2:5, is stirred evenly at normal temperature, and precooling processing is freeze-dried afterwards for 24 hours at -18 DEG C, final to obtain junket egg
White-procyanidine nano-complex.
To embedding rate, average grain diameter, the polydispersity index of embodiment, the resulting procyanidine nano-complex of reference examples
PDI, Zeta potential and storage-stable compare, as shown in table 1:
1. embodiment and comparative example of table prepares the comparison of nano-complex
As shown in Table 1, by 28 days storage after, its embedding rate of casein-procyanidine nano-complex of reference examples with
Compared to being remarkably decreased, partial size dramatically increases embodiment casein-maltodextrin procyanidine nano-complex.
Through above-mentioned comparison it can be found that compared with casein-maltodextrin procyanidine is nano combined, pure junket egg is selected
After the white embedding to procyanidine, storage-stable is substantially reduced.This is because casein will lead to during long-term storage
Aggregate and precipitate, so that its partial size increases, meanwhile, the formation of protein aggregate leads to the unstability of nanocomposite structures, may
So that more procyanidine are diffused into protein surface, the reduction of embedding rate is caused.And maltodextrin is grafted on casein it
Afterwards, the space structure for changing casein to a certain extent has introduced biggish steric hindrance.The presence of polysaccharide chain acts as
Barrier between casein and casein prevents the bridging between albumen.It can be seen that casein-maltodextrin connects
Branch this amphipathic biopolymer of object has more effectively embedding effect to procyanidine.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (10)
1. a kind of preparation method of self assembly procyanidine nano-complex, which comprises the following steps:
Step 1: preparing casein and maltodextrin mixture
Casein and maltodextrin are added in phosphate buffer, is mixed with magnetic stirrer, obtains casein and wheat
The phosphate solution of bud dextrin, after being hydrated at room temperature, freeze-drying obtains casein and maltodextrin lyophilized products, spare；
Step 2: preparing casein-maltodextrin graft
Sieve is crossed after the casein and maltodextrin lyophilized products are pulverized, and is sealed with masking foil with holes, is placed in closed container
Middle progress self assembly and Maillard reaction, obtain casein-maltodextrin graft, deposit in spare at 4 DEG C；
Step 3: preparation procyanidine solution and casein-maltodextrin mixed solution
It weighs procyanidine to be dissolved in deionized water, magnetic agitation obtains procyanidine solution uniformly to being completely dissolved；It weighs
The casein-maltodextrin graft is dissolved in phosphate buffer, and it is molten to obtain casein-maltodextrin graft mixing
Liquid；
Step 4: preparation procyanidine nano-complex
The procyanidine solution is poured slowly into the casein-maltodextrin mixed solution, at normal temperature magnetic agitation
Uniformly, precooling processing is then carried out, then is freeze-dried, it is multiple finally to obtain casein-maltodextrin procyanidine nanometer
Close object.
2. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 1, the time being stirred is 1h, and the time of the hydration is that for 24 hours, the time of the freeze-drying is 48h.
3. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 1, the mass ratio of the casein and the maltodextrin is 1:2.
4. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 1, the concentration of the phosphate buffer is 0.2M, and pH is 6.0~8.0.
5. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 1, in the phosphate solution of the casein and maltodextrin, the casein concentration is adjusted to 0.5%~1% (w/v).
6. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 2, the sieve mesh number is 80 mesh.
7. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 2, the condition of the self assembly and Maillard reaction is relative humidity 79%, temperature 60 C, 15~25h of time.
8. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 3, controlling the casein-maltodextrin graft mixed liquid concentration is 1%~2.5% (w/v).
9. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In 3, the weighed procyanidine and the casein-maltodextrin graft mass ratio are 2:5.
10. the preparation method of self assembly procyanidine nano-complex as described in claim 1, which is characterized in that the step
In rapid 4, the temperature of the precooling processing is -18 DEG C, and the time is for 24 hours.
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