CN106360437A - Krill oil microcapsule with antioxidation effect and preparation process thereof - Google Patents
Krill oil microcapsule with antioxidation effect and preparation process thereof Download PDFInfo
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Abstract
The invention relates to the technical field of health care food, in particular to a krill oil microcapsule with an antioxidation effect and a preparation process thereof. Antarctic krill oil, DHA algal oil and haematococcus pluvialis are used as core materials; a proper amount carrageenan is added into an oil phase; chitosan oligosaccharide and gamma-cyclodextrin are used as wall materials, and are proportionally mixed with an oil phase antioxidant, a water phase antioxidant, an emulsifying agent, a dispersing agent and the like to be prepared into microemulsion; through freeze drying, powder grinding, sieving and the like, the krill oil microcapsule is prepared. The krill oil microcapsule obtained by the process has the advantages that the stability of the krill oil on the factors such as light, temperature and humidity is improved; the content of bioactive substances such as astaxanthin and DHA in the microcapsule is high; the antioxidation performance of the krill oil is greatly improved.
Description
Technical field
The present invention relates to technical field of health care food and in particular to a kind of krill oil microcapsule with antioxidation and
Its preparation technology.
Background technology
Antarctic krill oil is to be refined by Antarctic krill to form, and existing research is proved antarctic krill oil and is rich in multiple unsaturations
Fatty acid, containing abundant epa, dha, astaxanthin, phospholipid etc., wherein omega-3 polyunsaturated fatty acidses epa and dha content very
Height, accounts for 15.86%, and the ratio of epa and dha is up to 15:1.Different from ω -3 triglyceride form in fish oil and cod-liver oil, south
The omega-fatty acid of pole krill oil is attached on phospholipid, can be fully absorbed by small intestinal, increased bioavailability, and krill oil
Intrinsic natural powerful antioxidant astaxanthin be also attached to phospholipid, thus creating more stable lasting antioxidation effect
Power.
Antarctic krill oil not only has stronger antioxidation, removes free radical, the effect of active cell vigor, but also has
There are blood fat reducing, prevention of arterial hardening, improve the functions such as memory, also have the advantages that quickly to be absorbed by human body simultaneously.
Haematocoocus Pluvialls contain abundant astaxanthin, and dha algae oil is rich in dha, and both can be notable with antarctic krill oil collocation
Improve astaxanthin and dha content, thus greatly strengthening the non-oxidizability of antarctic krill oil, removing free radical, improve memory etc.
The activity of aspect.
But antarctic krill oil is rich in the strong anti-oxidation material such as ω -3 grade polyunsaturated fatty acid, astaxanthin, therefore easily
Affected and occur oxidation to generate the harmful substances such as aldehyde, ketone by light, temperature, oxygen, water grading factors, this is not only caused South Pole phosphorus
Shrimp sauce colour-darkening, produces niff, and it can be made because becoming sour to lose original nutrition and health care and be worth.
Prevent the mode that antarctic krill oil becomes sour from mainly having two kinds at present, a kind of is the mode adding antioxidant solving
Antarctic krill oil becomes sour, and the method can not effectively solving antarctic krill oil be become sour problem；Another kind is to embed krill oil, adopts
With embedding to antarctic krill oil, make antarctic krill oil microcapsule powder, be dried using spray drying method in this technique, this process
Astaxanthin, dha, epa can be caused to lose in a large number, so that antarctic krill oil loses original healthy nutritive value.
The oxidation-resistant active ingredients such as astaxanthin, dha are caused to lose in a large number for the above-mentioned antarctic krill oil of solution easily becomes sour rotten
The problems such as mistake, it is an object of the invention to provide a kind of krill oil microcapsule with antioxidation and its preparation technology.
Content of the invention
The present invention is directed to problems of the prior art, provide a kind of krill oil microcapsule with antioxidation and
Its preparation technology.
The present invention is achieved through the following technical solutions this purpose:
A kind of krill oil microcapsule with antioxidation, described krill oil microcapsule by core, wall material, dispersant,
Oil phase antioxidant, aqueous phase antioxidant, emulsifying agent composition；
Described core is made up of antarctic krill oil, dha algae oil, Haematocoocus Pluvialls；
Described wall material is gamma-cyclodextrin, oligochitosan, pectin, modified starch, Microcrystalline Cellulose, lactalbumin, soybean protein
In any one or two or more mixture；
Described dispersant is trehalose, glucose, sucrose, any one or two or more mixture in Lactose；
Described oil phase antioxidant be natural complex e, tea polyphenols, tea polyphenol-palmitate, Butylated hydroxyanisole, two
Any one in butylated hydroxytoluene or two or more mixture；
Described aqueous phase antioxidant is vitamin c, calcium ascorbate, d- sodium erythorbate, appointing in sodium ascorbate
Anticipate one or more mixture；
Described emulsifying agent is soybean phospholipid, methyl glycol fatty acid ester, succinylated monoglyceride, in polyglyceryl fatty acid ester
Any one or two or more mixture.
Wherein, described core by the antarctic krill oil of 50～85 weight portions, the dha algae oil of 0.1～10 weight portion, 0.1～
The Haematocoocus Pluvialls composition of 10 weight portions.
A kind of preparation technology of the krill oil microcapsule with antioxidation, comprises the following steps:
1) preparation of aqueous phase: by the wall material of 12～65 weight portions, the dispersant of 0.05～8 weight portion, 0.05～8 weight portion
Emulsifying agent, the aqueous phase antioxidant of 0.01～0.5 weight portion sequentially add in the water of 45～95 weight portions, stirring while adding,
Make its mix homogeneously, solution temperature is maintained at 50～95 DEG C, prepared aqueous phase；
2) preparation of oil phase: by the antarctic krill oil of 50～85 weight portions, the Haematocoocus Pluvialls of 0.1～10 weight portion, 0.1
The dha algae oil of～10 weight portions is sufficiently mixed prepared core, adds emulsifying agent, 0.01～0.5 weight portion of 0.05～8 weight portion
Oil phase antioxidant, stirring while adding so as to mix homogeneously, oil phase temperature is maintained at 50～80 DEG C, prepared oil phase；
3) preparation of microemulsion: oil phase is slowly added in aqueous phase, side edged shear, wherein rotating speed in 100000r/min,
Shearing 10min, repeats homogenizing three times, prepared microemulsion afterwards under the conditions of 45～60mpa；
4) krill oil microcapsule preparation: first microemulsion liquid temp is down to 1 DEG C, is subsequently placed in -35～-50 DEG C, pressure is
In 10～18mpa vacuum freezing drying oven, finally pressure is down to 0.5～3mpa, temperature is down to -55～-65 DEG C, keep 3～
5h, takes out and gained solid is dried, through beating powder, sieving, prepared krill oil microcapsule.
With respect to prior art, the invention has the benefit that
The present invention is with antarctic krill oil, dha algae oil, Haematocoocus Pluvialls as core, and adds suitable carrageenan in oil phase；
With oligochitosan, gamma-cyclodextrin as wall material, mix in proportion with oil phase antioxidant, aqueous phase antioxidant, emulsifying agent, dispersant etc.
Close, make microemulsion, freeze-dried, beat powder, sieve etc. and making krill oil microcapsule, this technique gained krill oil microcapsule
Not only increase the stability to factors such as light, temperature, humidity for the krill oil, and in microcapsule, astaxanthin and dha etc. are biological alive
Property content of material is higher, greatly improves the non-oxidizability of krill oil.
Specific embodiment
Describe the present invention below in conjunction with specific embodiment.
Embodiment 1.
The present embodiment provides a kind of preparation technology of the krill oil microcapsule with antioxidation, comprises the following steps:
1) preparation of aqueous phase: by 50kg modified starch, 14.6kg shitosan, 0.05kg trehalose, 0.05kg sucrose,
0.05kg succinylated monoglyceride, 0.02kg calcium ascorbate sequentially add in 260kg water, stirring while adding so as to mixing all
Even, solution temperature should be maintained at 50～95 DEG C, prepared aqueous phase；
2) preparation of oil phase: by 26kg antarctic krill oil, 7kg Haematocoocus Pluvialls, 3kg dha algae oil, 0.05kg succinic acid
Monoglyceride, 0.02kg tea polyphenols add successively, stirring while adding so as to mix homogeneously, oil phase temperature should be maintained at 50～80
DEG C, prepared oil phase；
3) preparation of microemulsion: oil phase is slowly added in aqueous phase, side edged shear, wherein rotating speed in 100,000 turns/min,
Shearing 10min, repeats homogenizing three times, prepared microemulsion afterwards under the conditions of 45mpa；
4) krill oil microcapsule preparation: first microemulsion liquid temp is down to 1 DEG C, is subsequently placed in -40 DEG C, pressure is 12mpa
In vacuum freezing drying oven, finally pressure is down to 1mpa, temperature is down to -65 DEG C, keeps 3～5h, take out and gained solid is dried,
Through beating powder, sieving, prepared krill oil microcapsule.
Embodiment 2.
1) preparation of aqueous phase: by 30kg gamma-cyclodextrin, 7kg soybean protein isolate, 1kg trehalose, 0.5kg sucrose, 1kg
Soybean phospholipid, 0.5kg natural complex c sequentially add in 280kg water, stirring while adding so as to mix homogeneously, solution temperature should
It is maintained at 75 DEG C, prepared aqueous phase；
2) preparation of oil phase: by 44kg antarctic krill oil, 12kg Haematocoocus Pluvialls, 4.5kg dha algae oil, 1kg Semen sojae atricolor phosphorus
Fat, 0.5kg natural complex e add successively, and stirring while adding oil phase temperature should be maintained at 60 DEG C so as to mix homogeneously, is obtained
Oil phase；
3) preparation of microemulsion: oil phase is slowly added in aqueous phase, side edged shear, wherein rotating speed in 100,000 turns/min,
Shearing 10min, repeats homogenizing three times, prepared microemulsion afterwards under the conditions of 55mpa；
4) krill oil microcapsule preparation: first microemulsion liquid temp is down to 1 DEG C, is subsequently placed in -45 DEG C, pressure is 15mpa
In vacuum freezing drying oven, finally pressure is down to 2mpa, temperature is down to -60 DEG C, keeps 3～5h, take out and gained solid is dried,
Through beating powder, sieving, prepared krill oil microcapsule.
Embodiment 3.
1) preparation of aqueous phase: by 12kg Microcrystalline Cellulose, 5kg pectin, 0.5kg glucose, 0.5kg Lactose, 0.25kg third
Diol fatty acid ester, 0.5kg d- arabo-ascorbic acid sequentially add in 200kg water, stirring while adding so as to mix homogeneously, solution
Temperature should be maintained at 90 DEG C, prepared aqueous phase；
2) preparation of oil phase: by 58kg antarctic krill oil, 16kg Haematocoocus Pluvialls, 6kg dha algae oil, 0.25kg propylene glycol
Fatty acid ester, 0.5kg bht add successively, and stirring while adding oil phase temperature should be maintained at 80 DEG C so as to mix homogeneously, is obtained
Oil phase；
3) preparation of microemulsion: oil phase is slowly added in aqueous phase, side edged shear, wherein rotating speed in 100,000 turns/min,
Shearing 10min, repeats homogenizing three times, prepared microemulsion afterwards under the conditions of 60mpa；
4) krill oil microcapsule preparation: first microemulsion liquid temp is down to 1 DEG C, is subsequently placed in -50 DEG C, pressure is 18mpa
In vacuum freezing drying oven, finally pressure is down to 3mpa, temperature is down to -65 DEG C, keeps 3～5h, take out and gained solid is dried,
Through beating powder, sieving, prepared krill oil microcapsule.
Antioxidation checking test.
1. materials and methods
1.1 reagent and instrument: given the test agent is the krill oil microcapsule of preparation in embodiment 2；Water used by experiment is purification
Water；Mda, sod, gsh index detection kit builds up Bioengineering Research Institute purchased from Nanjing respectively；
1.2 laboratory animals: from 10 monthly ages above rat 40, single sex, weight average 380g ± 5g；
1.3 packets: press mda level packet in blood, 40 rats are randomly divided into 1 blank control group and 3 test samples
Product dosage group, every group 10.3 dosage groups give variable concentrations given the test agent, and matched group gives same volume solvent, concrete such as table
Shown in 1.
Table 1
| Group | Size of animal (only) | Dosage (gram/day) |
| Blank control group | 10 | 0 |
| Low dose group | 10 | 1.5 |
| Middle dose group | 10 | 3.0 |
| High dose group | 10 | 6.0 |
1.4 index determinings: at the end of experiment put to death animal survey lipid oxidation products content, reductive glutathione content,
Antioxidase activity.
Experimental technique is carried out in strict accordance with method specified in kit specification, respectively measure serum in mda, sod,
Gsh content or vigor.
2. data processing and result judgement
Experimental data adopts variance analyses, experimental group and blank control group relative analyses, three experimental group relative analyses.
2.1 experimental grouies and blank control group relative analyses result: basic, normal, high three dosage experiments group rat blood serum sod,
Gsh vigor all significantly improves than blank control group vigor, p ＜ 0.05；Three dosage experiments group rat blood serum mad contents are all than sky
White matched group mad content significantly reduces, and p ＜ 0.05 illustrates that in embodiment, three dosage of krill oil microcapsule all have antioxidation
Effect；
Relative analyses result between 2.2 3 dosage experiments groups: high, middle dose group compared with low dose group, high, middle dose group
Rat blood serum sod, gsh vigor significantly improves than low dose group rat blood serum sod, gsh vigor, p ＜ 0.05；High, middle dose group
Rat blood serum mad content significantly reduces than low dose group rat blood serum mad content, p ＜ 0.05；High dose group rat blood serum sod,
Gsh vigor somewhat increases than middle dose group rat blood serum sod, gsh vigor, and high dose group rat blood serum mad content compares middle dosage
Group rat blood serum mad content significantly reduces, and p ＜ 0.05 illustrates krill oil microcapsule in embodiment, within the specific limits, with
Dosage increases, and antioxidation strengthens.
2.3 result judgements: by experimental group and matched group contrast, three dosage group relative analyses, result shows embodiment
Middle krill oil microcapsule can significantly reduce mad content in rat blood serum, can significantly improve sod, gsh vigor in rat blood serum, from
And can determine that krill oil microcapsule has antioxidation.
In other embodiment, gained krill oil microcapsule equally has above-mentioned experimental result, can determine that the krill oil of the present invention
Microcapsule has antioxidation.
Embodiment described above only have expressed the several embodiments of the present invention, and its description is more concrete and detailed, but simultaneously
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, some deformation can also be made and improve, these broadly fall into the guarantor of the present invention
Shield scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (3)
1. a kind of krill oil microcapsule with antioxidation is it is characterised in that described krill oil microcapsule is by core, wall
Material, dispersant, oil phase antioxidant, aqueous phase antioxidant, emulsifying agent composition；
Described core is made up of antarctic krill oil, dha algae oil, Haematocoocus Pluvialls；
Described wall material is gamma-cyclodextrin, oligochitosan, pectin, modified starch, Microcrystalline Cellulose, lactalbumin, in soybean protein
Any one or two or more mixture；
Described dispersant is trehalose, glucose, sucrose, any one or two or more mixture in Lactose；
Described oil phase antioxidant is natural complex e, tea polyphenols, tea polyphenol-palmitate, Butylated hydroxyanisole, dibutyl
Any one in hydroxy-methylbenzene or two or more mixture；
Described aqueous phase antioxidant is vitamin c, calcium ascorbate, d- sodium erythorbate, any one in sodium ascorbate
Plant or two or more mixture；
Described emulsifying agent is soybean phospholipid, methyl glycol fatty acid ester, succinylated monoglyceride, any in polyglyceryl fatty acid ester
One or more mixture.
2. the krill oil microcapsule with antioxidation according to claim 1 is it is characterised in that described core is by 50
The antarctic krill oil of～85 weight portions, the dha algae oil of 0.1～10 weight portion, the Haematocoocus Pluvialls composition of 0.1～10 weight portion.
3. the preparation technology of the krill oil microcapsule with antioxidation according to claim 1 is it is characterised in that wrap
Include following steps:
1) preparation of aqueous phase: by the wall material of 12～65 weight portions, the dispersant of 0.05～8 weight portion, 0.05～8 weight portion breast
Agent, the aqueous phase antioxidant of 0.01～0.5 weight portion sequentially add in the water of 45～95 weight portions, stirring while adding so as to
Mix homogeneously, solution temperature is maintained at 50～95 DEG C, prepared aqueous phase；
2) preparation of oil phase: by the antarctic krill oil of 50～85 weight portions, the Haematocoocus Pluvialls of 0.1～10 weight portion, 0.1～10
The dha algae oil of weight portion is sufficiently mixed prepared core, adds emulsifying agent, the oil of 0.01～0.5 weight portion of 0.05～8 weight portion
Phase antioxidant, stirring while adding so as to mix homogeneously, oil phase temperature is maintained at 50～80 DEG C, prepared oil phase；
3) preparation of microemulsion: oil phase is slowly added in aqueous phase, side edged is sheared, wherein rotating speed, in 100000r/min, is sheared
10min, repeats homogenizing three times, prepared microemulsion afterwards under the conditions of 45～60mpa；
4) krill oil microcapsule preparation: first microemulsion liquid temp is down to 1 DEG C, is subsequently placed in -35～-50 DEG C, pressure be 10～
In 18mpa vacuum freezing drying oven, finally pressure is down to 0.5～3mpa, temperature is down to -55～-65 DEG C, keeps 3～5h, take
Go out to be dried gained solid, through beating powder, sieving, prepared krill oil microcapsule.
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| CN107232636A (en) * | 2017-07-28 | 2017-10-10 | 美泰科技（青岛）股份有限公司 | A kind of preparation method of water-soluble antarctic krill oil microcapsule powder |
| CN107897466A (en) * | 2017-11-09 | 2018-04-13 | 山东天地健生物工程有限公司 | A kind of antarctic krill oil interlayer type gel candy and preparation method thereof |
| CN107981144A (en) * | 2017-11-23 | 2018-05-04 | 成都朴锐生物科技有限公司 | The preparation process of haematococcus pluvialis extract solid beverage |
| CN108013441A (en) * | 2017-11-29 | 2018-05-11 | 华南理工大学 | A kind of astaxanthin embedding compound and preparation method and its application in functional yoghourt |
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| CN107897466A (en) * | 2017-11-09 | 2018-04-13 | 山东天地健生物工程有限公司 | A kind of antarctic krill oil interlayer type gel candy and preparation method thereof |
| CN107981144A (en) * | 2017-11-23 | 2018-05-04 | 成都朴锐生物科技有限公司 | The preparation process of haematococcus pluvialis extract solid beverage |
| CN108013441A (en) * | 2017-11-29 | 2018-05-11 | 华南理工大学 | A kind of astaxanthin embedding compound and preparation method and its application in functional yoghourt |
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| CN113115843A (en) * | 2019-12-30 | 2021-07-16 | 丰益(上海)生物技术研发中心有限公司 | Structured emulsions containing algal oil |
| CN113115843B (en) * | 2019-12-30 | 2023-12-19 | 丰益(上海)生物技术研发中心有限公司 | Structured emulsions containing algae oil |
| CN117530446A (en) * | 2023-11-09 | 2024-02-09 | 逢时(青岛)海洋科技有限公司 | Krill oil microemulsion gel bead and preparation method thereof |
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