CN107041554B - Beta-carotene microcapsule rich in sardine oil and preparation method and application thereof - Google Patents
Beta-carotene microcapsule rich in sardine oil and preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a beta-carotene microcapsule rich in sardine oil and a preparation method and application thereof. The method comprises the steps of sequentially adding modified starch, chitosan, trehalose, sucrose and succinic acid monoglyceride into water, stirring while adding, uniformly mixing, keeping the solution at a certain temperature to prepare a water phase, adding beta-carotene crystals, lipophilic emulsifiers Tween and sardine oil into an oil phase kettle, stirring for dissolving to obtain an oil phase, slowly adding the oil phase into the water phase, preparing a micro-emulsion through shearing, concentrating, freeze-drying, grinding and the like to prepare the beta-carotene microcapsule rich in sardine oil. Compared with the prior art, the beta-carotene microcapsule provided by the invention not only improves the stability of the beta-carotene to factors such as light, heat and humidity, but also is rich in sardine oil, and can play a synergistic effect with the beta-carotene, so that the health-care performance of the microcapsule is improved.
Description
Technical Field
The invention relates to the technical field of health-care food, in particular to a beta-carotene microcapsule rich in sardine oil and a preparation method and application thereof.
Background
The beta-carotene is one of carotenoids, is an orange fat-soluble compound, belongs to a tetraterpenoid compound, can effectively eliminate free radicals, reduce the incidence rate of cancers, and also has the effects of inhibiting cataract, preventing arteriosclerosis and resisting aging. However, during food processing and storage, beta-carotene has poor stability, is very easily oxidized, and is easily isomerized due to the action of heat, acid and light. In addition, the weak polar molecular structure determines that the beta-carotene has the properties of insolubility in water and slight solubility in grease, and limits the application range of the beta-carotene. The beta-carotene oral liquid prepared in the actual production has great limitation in the application in the food industry due to poor taste and poor stability. The existing method for solving the problems mainly adopts microcapsule coating, beta-carotene is embedded in a macromolecular membrane material by a microcapsule technology to play a role in protection, and the beta-carotene which is insoluble in water has the characteristics of good redispersibility, easy water dissolution, no solution layering and the like after being microencapsulated.
However, the research on microencapsulation of β -carotene has mainly focused on how to increase the coating rate, and how to coat several bioactive substances in the microcapsule together with β -carotene to exert a synergistic effect has been rarely studied.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a beta-carotene microcapsule rich in sardine oil and a preparation method and application thereof.
The purpose of the invention can be realized by the following technical scheme:
a preparation method of beta-carotene microcapsules rich in sardine oil comprises the following steps:
(1) preparation of an aqueous phase: sequentially adding modified starch, chitosan, trehalose, sucrose and succinic acid monoglyceride into water while stirring, mixing, and maintaining the solution at a certain temperature to obtain water phase;
(2) preparing an oil phase: adding beta-carotene crystals, lipophilic emulsifier Tween and sardine oil into an oil phase kettle, and stirring and dissolving to obtain an oil phase;
(3) preparing microemulsion: slowly adding the oil phase into the water phase, and shearing to obtain microemulsion;
(4) preparing microcapsules: firstly, cooling the microemulsion temperature, then placing the microemulsion in a freeze drying oven, drying, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
In the step (1), the mass ratio of the modified starch, the chitosan, the trehalose, the sucrose, the succinic acid monoglyceride and the water is (30-80): (5-30): (0.03-0.08): (0.03-0.08): (0.03-0.08): 260.
in the step (1), the temperature of the solution is 40-95 ℃.
In the step (2), the mass ratio of the beta-carotene crystal, the lipophilic emulsifier Tween and the sardine oil is (5-20): 1: (20 to 50).
In the step (2), the temperature of the oil phase is 30-80 ℃.
In the step (3), after the oil phase is slowly added into the water phase, the mass ratio of the beta-carotene crystals to the modified starch is (5-20): (30-80).
In the step (3), the microemulsion preparation conditions are as follows: the shearing rotating speed is 5-20 ten thousand revolutions per minute, the shearing time is 5-20 minutes, the shearing pressure is 30-60 Mpa, and the repeated shearing time is 2-5 times.
In the step (4), the preparation conditions of the microcapsule are as follows: firstly, cooling the temperature of the microemulsion to-5 to-20 ℃, then placing the microemulsion in a freeze drying box at-30 to 60 ℃, drying the microemulsion, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
The beta-carotene microcapsule rich in sardine oil can be used as health food for middle-aged and elderly people, and has effects of scavenging free radicals, resisting oxidation and protecting cardiovascular system.
In the invention, the sardine oil is an extract from sardine fat, contains polyunsaturated fatty acid as a main component, is rich in EPA and DHA, can reduce low-density lipoprotein in human blood, reduce blood viscosity, prevent blood plaque formation, has the auxiliary effects of reducing blood fat and treating cardiovascular and cerebrovascular diseases, and can prevent senile dementia from occurring by using DHA and EPA.
Beta-carotene is one of the most effective antioxidants against free radicals, and is capable of preventing arteriosclerosis and anti-aging. The beta-carotene and the sardine oil have the effects on the aspect of preventing vascular diseases, the synergistic effect of the beta-carotene and the sardine oil can be better played, oil needs to be added in the emulsification process of the beta-carotene, and the sardine oil can be used as an emulsification aid and an effective component. Therefore, the prepared beta-carotene microcapsule rich in sardine oil can be used as a health food for the middle-aged and the elderly.
Compared with the prior art, the invention has the advantages of simple and feasible process and easy industrialization, and the microcapsule simultaneously comprises the double functional components of sardine oil and beta-carotene.
Sardine oil is rich in EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid), both of which are unsaturated fatty acids, can be easily converted into High Density Lipoprotein (HDL) in human body to exert physiological functions, and has the functions of regulating blood lipid, clearing thrombus, preventing senile dementia, nourishing brain, improving memory, improving eyesight, preventing and treating presbyopia, etc. Beta-carotene can be converted into vitamin A in vivo, is the most safe product for supplementing the vitamin A at present, can maintain the health of eyes and skin, improve the conditions of nyctalopia and rough skin, promote growth and development, effectively promote health and cell development, help the body to be free from the damage of free radicals, maintain and promote immune function, and can play a synergistic role in nutrition and health care.
The product obtained by the invention improves the stability of the beta-carotene to factors such as light, heat, humidity and the like, and after the microcapsule is formed by coating sardine grease and the like, the air, oxygen, water vapor and the like are isolated, the light irradiation and the like are blocked, and the oxidative deterioration rate of the beta-carotene is greatly reduced.
Therefore, the product obtained by the invention not only improves the stability of the beta-carotene to factors such as light, heat, humidity and the like, but also is convenient to carry, store and eat, and the prepared beta-carotene microcapsule rich in sardine oil is slightly influenced by the rhythm of food delivery in the digestive (digestive food) tract due to small particle size, is easy to absorb in the gastrointestinal environment and has the advantage of high bioavailability.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
(1) Preparation of an aqueous phase: sequentially adding 50kg of modified starch, 15kg of chitosan, 0.05kg of trehalose, 0.05kg of sucrose and 0.05kg of succinic acid monoglyceride into 260kg of water, stirring while adding, and uniformly mixing, wherein the temperature of the solution is kept at 60 ℃ to prepare a water phase;
(2) preparing an oil phase: 25kg of beta-carotene crystals, 2kg of lipophilic emulsifier and 50kg of sardine oil are added into an oil phase kettle and stirred and dissolved at 60 ℃ to obtain an oil phase.
(3) Preparing microemulsion: slowly adding the oil phase into the water phase while shearing, wherein the rotating speed is 10 ten thousand revolutions per minute, after shearing for 10 minutes, repeatedly homogenizing for three times under the condition of 45Mpa to prepare the microemulsion;
(4) preparing microcapsules: firstly, cooling the temperature of the microemulsion to-10 ℃, then placing the microemulsion in a freeze drying oven at-40 ℃, drying, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
Example 2
(1) Preparation of an aqueous phase: sequentially adding 40kg of modified starch, 10kg of chitosan, 0.06kg of trehalose, 0.04kg of sucrose and 0.08kg of succinic acid monoglyceride into 260kg of water while stirring, and uniformly mixing, wherein the temperature of the solution is kept at 70 ℃ to prepare a water phase;
(2) preparing an oil phase: 20kg of beta-carotene crystals, 2kg of lipophilic emulsifier and 60kg of sardine oil are added into an oil phase kettle and stirred and dissolved at 50 ℃ to obtain an oil phase.
(3) Preparing microemulsion: slowly adding the oil phase into the water phase while shearing, wherein the rotating speed is 12 ten thousand revolutions per minute, after shearing for 10 minutes, repeatedly homogenizing for four times under the condition of 40Mpa to prepare the microemulsion;
(4) preparing microcapsules: firstly, cooling the temperature of the microemulsion to-10 ℃, then placing the microemulsion in a freeze drying oven at-40 ℃, drying, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
Example 3
(1) Preparation of an aqueous phase: sequentially adding 50kg of modified starch, 15kg of chitosan, 0.05kg of trehalose, 0.04kg of sucrose and 0.08kg of succinic acid monoglyceride into 260kg of water while stirring, and uniformly mixing, wherein the temperature of the solution is kept at 70 ℃ to prepare a water phase;
(2) preparing an oil phase: 20kg of beta-carotene crystals, 2kg of lipophilic emulsifier and 60kg of sardine oil are added into an oil phase kettle and stirred and dissolved at 50 ℃ to obtain an oil phase.
(3) Preparing microemulsion: slowly adding the oil phase into the water phase while shearing, wherein the rotating speed is 10 ten thousand revolutions per minute, and repeatedly homogenizing for four times under the condition of 60Mpa after shearing for 10 minutes to prepare the microemulsion;
(4) preparing microcapsules: firstly, cooling the temperature of the microemulsion to-20 ℃, then placing the microemulsion in a freeze drying oven at-50 ℃, drying, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
Example 4
Verification of antioxidation
(1) Taking the beta-carotene microcapsules rich in sardine oil prepared in example 1, feeding 40 male rats of 10 months of age, the weight of the male rats is 360g +/-5 g on average, and randomly dividing the 40 rats into 1 blank control group and 3 sample-receiving dose groups (feeding a low dose of 1g, a medium dose of 2g and a low dose of 4g respectively every day);
(2) after 15 days of feeding, the animals were sacrificed for the determination of the MAD content, SOD and GSH activity in the serum, and the results are shown in Table 1;
(3) according to the experimental data in table 1, compared with a blank control group, the analysis results show that the activities of the rat serum SOD and GSH of the experimental group with low, medium and high doses are obviously improved compared with the activities of the blank control group, the MAD content is obviously reduced compared with the MAD content of the blank control group, and the three doses of the beta-carotene microcapsules rich in sardine oil have anti-oxidation effect in the embodiment;
(4) according to the experimental data in table 1, compared with the low dose group, the activities of the serum SOD and the GSH of the rats in the high and medium dose groups are obviously improved compared with the activities of the serum SOD and the GSH of the rats in the low dose group; the serum MAD content of the rats in the high and medium dose groups is obviously reduced compared with that of the rats in the low dose group; the activities of the serum SOD and the GSH of the high-dose group of rats are slightly increased compared with the activities of the serum SOD and the GSH of the medium-dose group of rats, and the content of the serum MAD of the high-dose group of rats is obviously reduced compared with the content of the serum MAD of the medium-dose group of rats, which shows that the beta-carotene microcapsule rich in sardine oil in the embodiment has enhanced antioxidant effect along with the increase of the dose within a certain range;
TABLE 1 SOD, GSH activity and MDA content in serum of each group of mice
The same experimental results as described above were obtained for the β -carotene microcapsules rich in sardine oil obtained in examples 2 and 3 according to the method of example 4, and it was confirmed that the β -carotene microcapsules rich in sardine oil according to the present invention have antioxidant health effects.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (1)
1. The application of the beta-carotene microcapsule rich in sardine oil in preparing health-care food for the middle-aged and the elderly is characterized in that the beta-carotene microcapsule rich in sardine oil is obtained by adopting the following preparation method:
(1) preparation of an aqueous phase: sequentially adding 50kg of modified starch, 15kg of chitosan, 0.05kg of trehalose, 0.05kg of sucrose and 0.05kg of succinic acid monoglyceride into 260kg of water, stirring while adding, and uniformly mixing, wherein the temperature of the solution is kept at 60 ℃ to prepare a water phase;
(2) preparing an oil phase: adding 25kg of beta-carotene crystals, 2kg of lipophilic emulsifier and 50kg of sardine oil into an oil phase kettle, and stirring and dissolving at 60 ℃ to obtain an oil phase;
(3) preparing microemulsion: slowly adding the oil phase into the water phase while shearing, wherein the rotating speed is 10 ten thousand revolutions per minute, after shearing for 10 minutes, repeatedly homogenizing for three times under the condition of 45Mpa to prepare the microemulsion;
(4) preparing microcapsules: firstly, cooling the temperature of the microemulsion to-10 ℃, then placing the microemulsion in a freeze drying oven at-40 ℃, drying, taking out the obtained solid, and preparing the beta-carotene microcapsule rich in sardine oil by powdering and sieving.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710303627.8A CN107041554B (en) | 2017-05-03 | 2017-05-03 | Beta-carotene microcapsule rich in sardine oil and preparation method and application thereof |
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| CN1545923A (en) * | 2003-12-03 | 2004-11-17 | 广州优宝工业有限公司 | Natural beta-carotene microcapsule and preparation method thereof |
| CN101703254A (en) * | 2009-12-10 | 2010-05-12 | 刘建敏 | Nutrient supplement food for use in dietary supplement method for rehabilitation therapy of cardiovascular and cerebrovascular diseases |
| CN103652931A (en) * | 2012-08-31 | 2014-03-26 | 武汉蜀泰科技有限公司 | Microcapsule formula for drink |
| CN104719894A (en) * | 2015-03-24 | 2015-06-24 | 武汉志邦化学技术有限公司 | Beta-carotene microcapsule and preparation technology thereof |
| CN105747216A (en) * | 2016-03-03 | 2016-07-13 | 广州市食品工业研究所有限公司 | Microcapsules-beta and carotene powder and preparation method thereof |
| CN106360437A (en) * | 2016-11-30 | 2017-02-01 | 广州康琪莱生物科技有限公司 | Krill oil microcapsule with antioxidation effect and preparation process thereof |
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| CN1545923A (en) * | 2003-12-03 | 2004-11-17 | 广州优宝工业有限公司 | Natural beta-carotene microcapsule and preparation method thereof |
| CN101703254A (en) * | 2009-12-10 | 2010-05-12 | 刘建敏 | Nutrient supplement food for use in dietary supplement method for rehabilitation therapy of cardiovascular and cerebrovascular diseases |
| CN103652931A (en) * | 2012-08-31 | 2014-03-26 | 武汉蜀泰科技有限公司 | Microcapsule formula for drink |
| CN104719894A (en) * | 2015-03-24 | 2015-06-24 | 武汉志邦化学技术有限公司 | Beta-carotene microcapsule and preparation technology thereof |
| CN105747216A (en) * | 2016-03-03 | 2016-07-13 | 广州市食品工业研究所有限公司 | Microcapsules-beta and carotene powder and preparation method thereof |
| CN106360437A (en) * | 2016-11-30 | 2017-02-01 | 广州康琪莱生物科技有限公司 | Krill oil microcapsule with antioxidation effect and preparation process thereof |
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