CN107041554A - Bata-carotene microcapsules rich in pilchard oil and preparation method and application - Google Patents
Bata-carotene microcapsules rich in pilchard oil and preparation method and application Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to bata-carotene microcapsules rich in pilchard oil and preparation method and application.Converted starch, chitosan, trehalose, sucrose, succinylated monoglyceride are added sequentially in water by the present invention, it is stirring while adding, it is well mixed, solution is maintained at certain temperature, aqueous phase is made, beta carotene crystal, oleophilic emulsifier tween and pilchard oil are added in oil phase kettle, oil phase is obtained after being stirred dissolving, oil phase is slowly added in aqueous phase, microemulsion is made by shearing, microemulsion is concentrated, the bata-carotene microcapsules rich in pilchard oil are made in freeze-drying, grinding etc..Compared with prior art, gained bata-carotene microcapsules of the invention not only increase stability of the bata-carotene to factors such as light, heat, humidity, and pilchard oil is rich in microcapsules, it can play and act synergistically with bata-carotene, improve the health properties of microcapsules.
Description
Technical field
The present invention relates to technical field of health care food, more particularly, to a kind of micro- glue of the beta carotene rich in pilchard oil
Capsule and preparation method and application.
Background technology
Beta carotene is one of carotenoid, is Chinese red fat-soluble compound, belongs to tetraterpenes compound, β-Hu
Radish element can effectively remove free radical, reduce the incidence of disease of cancer, also with suppression cataract, prevent artery sclerosis and anti-
Aging is acted on.But in food processing, storage, beta carotene stability is poor, easily it is oxidized, easily because of heat, acid
Effect with light and occur isomerization.In addition, low pole molecular structure determine beta carotene have it is water insoluble, be slightly soluble in
The property of grease, limits its application.Obtained beta carotene oral liquid is due to poor taste, stability in actual production
It is bad, its application in the food industry is had significant limitation.Solving the method for these problems now mainly has using micro-
Capsule is coated, and beta carotene is embedded in macromolecular membrane material, can played a protective role by microcapsules technology, and makes insoluble
In water beta carotene after microencapsulation, with the features such as redispersibility is good, soluble in water, solution is not stratified.
However, the research for beta carotene microencapsulation is mainly concentrated in terms of raising clad ratio at present, it is right
In how to be coated on several materials with bioactivity in microcapsules together with beta carotene, grinding for synergy is played
Study carefully seldom.
The content of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide one kind is rich in pilchard oil
Beta carotene microcapsules and preparation method and application.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of preparation method of the beta carotene microcapsules rich in pilchard oil, comprises the following steps：
(1) prepared by aqueous phase：Converted starch, chitosan, trehalose, sucrose, succinylated monoglyceride are added sequentially to water
In, it is stirring while adding, it is well mixed, solution is maintained at certain temperature, aqueous phase is made；
(2) prepared by oil phase：Beta carotene crystal, oleophilic emulsifier tween and pilchard oil are added in oil phase kettle, are carried out
Oil phase is obtained after stirring and dissolving；
(3) prepared by microemulsion：Oil phase is slowly added in aqueous phase, microemulsion is made by shearing；
(4) prepared by microcapsules：First by microemulsion greenhouse cooling, it is subsequently placed in freeze drying box, institute is taken out after drying
Solid is obtained, by playing powder, sieving, the beta carotene microcapsules rich in pilchard oil are made.
In step (1), converted starch, chitosan, trehalose, sucrose, the mass ratio of succinylated monoglyceride and water are (30
~80)：(5~30)：(0.03~0.08)：(0.03~0.08)：(0.03~0.08)：260.
In step (1), solution temperature is 40 DEG C~95 DEG C.
In step (2), the mass ratio of beta carotene crystal, oleophilic emulsifier tween and pilchard oil is (5~20)：1：
(20~50).
In step (2), oil phase temperature is 30 DEG C~80 DEG C.
In step (3), oil phase is slowly added to after aqueous phase, the mass ratio of beta carotene crystal and converted starch for (5~
20)：(30~80).
In step (3), microemulsion preparation condition is：Shearing rotating speed is 5~200,000 turns/min, shears 5~20min, shearing
Pressure is 30~60Mpa, and it is 2~5 times to repeat shearing number of times.
In step (4), the preparation condition of microcapsules is：Microemulsion temperature is down to -5~-20 DEG C first, be subsequently placed in -
In 30~60 DEG C of freeze drying box, gained solid is taken out after drying, by beating powder, sieving, the obtained β rich in pilchard oil-
Carrotene microcapsules.
Beta carotene microcapsules rich in pilchard oil can as person in middle and old age health food, with remove free radical,
Anti-oxidant and protection Cardiovascular.
In the present invention, pilchard oil is the extract from sardine fat, and main component is polybasic unsaturated fatty acid, rich
Containing EPA and DHA, it is possible to decrease the low-density lipoprotein in blood of human body, the stickiness of blood is reduced, anti-Hemostatic Oral Liquid patch is formed,
With reduction blood fat, the booster action of cardiovascular and cerebrovascular disease is treated, and DHA and EPA can also prevent senile dementia
Occur.
Beta carotene can prevent artery sclerosis and anti-aging to one of maximally effective antioxidant of Green Tea Extract.β-
Carrotene and pilchard oil all have effect in terms of vascular diseases are prevented, playing their cooperative effect can preferably send out
The effect of waving, and beta carotene needs to add grease in emulsion process, pilchard oil can not only be used for emulsion adjuvant, again can be with
It is used as functional component.Therefore, the beta carotene microcapsules rich in pilchard oil of preparation can be eaten as the health care of person in middle and old age
Product.
Compared with prior art, the present invention is easily industrialized with simple for process, and microcapsules include Sha Ding simultaneously
The advantage of fish oil and beta carotene double effects composition.
EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid) are rich in pilchard oil, both at unsaturated lipid
Fat acid, HDL (HDL) is easily converted into human body and plays physiological function, with regulation blood fat, clears up thrombus, in advance
Anti- senile dementia, nutrition brain, improvement memory, improve the functions such as eyesight, preventing and treating presbyopia.Beta carotene, in vivo can be with
Vitamin A is changed into, is current most safe replenishing vitamins A product, it can maintain the health of eyes and skin, improve night
Blind disease, the situation of pachylosis, enhancing development effectively facilitate health and cell development, contribute to body from free radical
Injury, maintain and promote immunologic function, in terms of nutrition and health care, can play its synergy.
Products obtained therefrom of the present invention improves stability of the beta carotene to factors such as light, heat, humidity, using pilchard oil
The cladding such as fat is formed after microcapsules, isolation air, oxygen, steam etc., is blocked light irradiation etc., is greatly reduced β-carrot
The speed of plain oxidation deterioration.
Therefore, the product that the present invention is obtained not only increases stability of the beta carotene to factors such as light, heat, humidity, and
And be easy to carry, be easy to preserve, instant, the beta carotene microcapsules rich in pilchard oil of preparation due to particle diameter it is small,
Influenceed small by digestion (digestion food) road conveying food rhythm and pace of moving things, be readily absorbed by gastrointestinal tract environment, it is high with bioavilability
Advantage.
Embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
(1) prepared by aqueous phase：By 50kg converted starches, 15kg chitosans, 0.05kg trehaloses, 0.05kg sucrose, 0.05kg
Succinylated monoglyceride is added sequentially in 260kg water, stirring while adding, is well mixed, solution temperature is maintained at 60 DEG C, is made
Aqueous phase；
(2) prepared by oil phase：25kg beta carotene crystal, 2kg oleophilic emulsifiers tween and 50kg pilchard oils is added
In oil phase kettle, oil phase is obtained after being stirred dissolving at 60 DEG C.
(3) prepared by microemulsion：Oil phase is slowly added in aqueous phase, the shearing of side edged, wherein rotating speed are 100,000 turns/min, are cut
Cut after 10min, then repeatedly homogeneous three times under the conditions of 45Mpa, microemulsion is made；
(4) prepared by microcapsules：Microemulsion temperature is down to -10 DEG C first, is subsequently placed in -40 DEG C of freeze drying box,
Gained solid is taken out after drying, by playing powder, sieving, the beta carotene microcapsules rich in pilchard oil are made.
Embodiment 2
(1) prepared by aqueous phase：By 40kg converted starches, 10kg chitosans, 0.06kg trehaloses, 0.04kg sucrose, 0.08kg
Succinylated monoglyceride is added sequentially in 260kg water, stirring while adding, is well mixed, solution temperature is maintained at 70 DEG C, is made
Aqueous phase；
(2) prepared by oil phase：20kg beta carotene crystal, 2kg oleophilic emulsifiers tween and 60kg pilchard oils is added
In oil phase kettle, oil phase is obtained after being stirred dissolving at 50 DEG C.
(3) prepared by microemulsion：Oil phase is slowly added in aqueous phase, the shearing of side edged, wherein rotating speed are 120,000 turns/min, are cut
Cut after 10min, then repeatedly homogeneous four times under the conditions of 40Mpa, microemulsion is made；
(4) prepared by microcapsules：Microemulsion temperature is down to -10 DEG C first, is subsequently placed in -40 DEG C of freeze drying box,
Gained solid is taken out after drying, by playing powder, sieving, the beta carotene microcapsules rich in pilchard oil are made.
Embodiment 3
(1) prepared by aqueous phase：By 50kg converted starches, 15kg chitosans, 0.05kg trehaloses, 0.04kg sucrose, 0.08kg
Succinylated monoglyceride is added sequentially in 260kg water, stirring while adding, is well mixed, solution temperature is maintained at 70 DEG C, is made
Aqueous phase；
(2) prepared by oil phase：20kg beta carotene crystal, 2kg oleophilic emulsifiers tween and 60kg pilchard oils is added
In oil phase kettle, oil phase is obtained after being stirred dissolving at 50 DEG C.
(3) prepared by microemulsion：Oil phase is slowly added in aqueous phase, the shearing of side edged, wherein rotating speed are 100,000 turns/min, are cut
Cut after 10min, then repeatedly homogeneous four times under the conditions of 60Mpa, microemulsion is made；
(4) prepared by microcapsules：Microemulsion temperature is down to -20 DEG C first, is subsequently placed in -50 DEG C of freeze drying box,
Gained solid is taken out after drying, by playing powder, sieving, the beta carotene microcapsules rich in pilchard oil are made.
Embodiment 4
Antioxidation is verified
(1) the beta carotene microcapsules rich in pilchard oil prepared in Example 1, the hero for feeding for 10 monthly ages
Property rat 40, weight average is 360g ± 5g, and 40 rats are randomly divided into 1 blank control group and 3 by sample dose
Group (feeds low dosage respectively daily：1g, middle dosage：2g and low dosage：4g)；
(2) after feeding 15 days, put to death animal and survey MAD contents, SOD and GSH vigor in serum, as a result as shown in table 1；
(3) according to the experimental data of table 1, experimental group is shown with blank control group comparative analysis result, basic, normal, high three agent
Amount experimental group rat blood serum SOD and GSH vigor is significantly improved than blank control group vigor, and MAD contents are than blank control MAD
Content is significantly reduced, and illustrates three equal Wheat Proteins of dosage of beta carotene microcapsules that pilchard oil is rich in embodiment；
(4) according to the experimental data of table 1, high, middle dose group compared with low dose group, height, middle dose group rat blood serum SOD,
GSH vigor is significantly improved than low dose group rat blood serum SOD, GSH vigor；High, middle dose group rat blood serum MAD contents are than low dose
Amount group rat blood serum MAD contents are significantly reduced；High dose group rat blood serum SOD, GSH vigor than middle dose group rat blood serum SOD,
GSH vigor somewhat increases, and high dose group rat blood serum MAD contents are significantly reduced than middle dose group rat blood serum MAD contents, explanation
The beta carotene microcapsules of pilchard oil are rich in embodiment, within the specific limits, as dosage increases, antioxidation increases
By force；
SOD, GSH activity and MDA contents in each group mice serum of table 1
It is same to beta carotene microcapsules of the gained rich in pilchard oil in embodiment 2,3 according to the method for embodiment 4
With above-mentioned experimental result, can determine that the beta carotene microcapsules rich in pilchard oil of the present invention has anti-oxidation health work
With.
The above-mentioned description to embodiment is understood that for ease of those skilled in the art and using invention.
Person skilled in the art obviously can easily make various modifications to these embodiments, and described herein general
Principle is applied in other embodiment without passing through performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
Field technique personnel are according to the announcement of the present invention, and not departing from improvement and modification that scope made all should be the present invention's
Within protection domain.
Claims (10)
1. a kind of preparation method of the beta carotene microcapsules rich in pilchard oil, it is characterised in that comprise the following steps：
(1) prepared by aqueous phase：Converted starch, chitosan, trehalose, sucrose, succinylated monoglyceride are added sequentially in water, side
Edged is stirred, and is well mixed, solution is maintained at certain temperature, and aqueous phase is made；
(2) prepared by oil phase：Beta carotene crystal, oleophilic emulsifier tween and pilchard oil are added in oil phase kettle, are stirred
Oil phase is obtained after dissolving；
(3) prepared by microemulsion：Oil phase is slowly added in aqueous phase, microemulsion is made by shearing；
(4) prepared by microcapsules：First by microemulsion greenhouse cooling, it is subsequently placed in freeze drying box, it is solid to take out gained after drying
Body, by playing powder, sieving, is made the beta carotene microcapsules rich in pilchard oil.
2. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In, in step (1), converted starch, chitosan, trehalose, sucrose, succinylated monoglyceride and water mass ratio for (30~
80)：(5~30)：(0.03~0.08)：(0.03~0.08)：(0.03~0.08)：260.
3. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (1), solution temperature is 40 DEG C~95 DEG C.
4. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (2), the mass ratio of beta carotene crystal, oleophilic emulsifier tween and pilchard oil is (5~20)：1：(20~
50)。
5. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (2), oil phase temperature is 30 DEG C~80 DEG C.
6. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (3), oil phase is slowly added to after aqueous phase, and the mass ratio of beta carotene crystal and converted starch is (5~20)：(30
~80).
7. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (3), microemulsion preparation condition is：Shearing rotating speed is 5~200,000 turns/min, shears 5~20min, and shear pressure is
30~60Mpa, it is 2~5 times to repeat shearing number of times.
8. a kind of preparation method of beta carotene microcapsules rich in pilchard oil according to claim 1, its feature exists
In in step (4), the preparation condition of microcapsules is：Microemulsion temperature is down to -5~-20 DEG C first, -30~60 are subsequently placed in
DEG C freeze drying box in, dry after take out gained solid, by beat powder, sieving, be made be rich in pilchard oil beta carotene
Microcapsules.
9. using the obtained beta carotene microcapsules for being rich in pilchard oil of method any one of claim 1-8.
10. the application of the beta carotene microcapsules rich in pilchard oil as claimed in claim 9, it is characterised in that rich in sand
The beta carotene microcapsules of fourth fish oil as person in middle and old age health food.
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