CN103789084A - Pine-seed oil and preparation method and use thereof - Google Patents
Pine-seed oil and preparation method and use thereof Download PDFInfo
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- CN103789084A CN103789084A CN201410053790.XA CN201410053790A CN103789084A CN 103789084 A CN103789084 A CN 103789084A CN 201410053790 A CN201410053790 A CN 201410053790A CN 103789084 A CN103789084 A CN 103789084A
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- separating tank
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- seed oil
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Abstract
The invention discloses pine-seed oil and a preparation method and use thereof. The pine-seed oil is obtained by carrying out supercritical CO2 extraction on pine nuts. The pine-seed oil disclosed by the invention can remarkably improve superoxide dismutase of the human body and reduce the degradation products of lipid peroxidase, has the function of delaying senescence, and can be used for preparing health-care products or medicines for delaying senescence.
Description
Technical field
The present invention relates to field of plant extraction, relate in particular to a kind of pine-seed oil and its production and use.
Background technology
Pine nut is the seed of pine tree, claims again Semen pini koraiensis.Pine nut is important Chinese medicine, eats for a long time the body-building heart, and skin care, promotes longevity, and also has very high food therapy value.
The nutritive value of pine nut is very high, is rich in fat, protein, carbohydrate etc.In every hectogram stuffed pork breast with pine kernels, containing 16.7 grams, protein, 63.5 grams, fat, the nutritive substances such as 78 milligrams of 9.8 grams, carbohydrate and mineral calciums, 236 milligrams, phosphorus, 6.7 milligrams of iron and unsaturated fatty acids.Fatty ingredient in pine nut is mainly the unsaturated fatty acids such as linolic acid, linolenic acid, has vessel softening and the atherosclerotic effect of control.Therefore, the elderly is edible pine nut often, has and prevents from increasing because of cholesterol the effect that causes cardiovascular disorder.In addition, phosphorous compared with horn of plenty in pine nut, also helpful to people's cerebral nerve.It,, to senile chronic bronchitis, bronchial asthma, constipation, rheumatic arthritis, neurasthenia and dizzy patient, all has certain auxiliary therapeutic action.
Summary of the invention
First the present invention provides a kind of pine-seed oil, in fact prepares by the following method:
Pine nut is carried out to supercritical CO
2extraction obtains.
Specifically, a kind of pine-seed oil of the present invention, prepares by the following method:
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 30-60 ℃ (preferred temperature is 35-45 ℃);
In the time that freezing tank temperature is below 0 ℃, open CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material;
The pressure that regulates separating tank 1 and separating tank 2 is 25-30MPa until pressure is the preferred pressure of 20-35MPa() time, carry out cycling extraction, and regulate CO
2flow be that 10-35kg/h(is preferably 25-35kg/h), keep constant temperature and pressure 1-3.5h(to be preferably 2-3h);
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
Supercritical CO of the present invention
2extraction is specifically by HA121-50-02 type supercritical CO
2extraction plant (Huaan, Jiangsu Province supercritical extraction company limited) is realized.
The present invention also provides the method for preparing above-mentioned pine-seed oil, and the method is:
Pine nut is carried out to supercritical CO
2extraction obtains.
Specifically, a kind of pine-seed oil of the present invention, prepares by the following method:
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 30-60 ℃ (preferred temperature is 35-45 ℃);
In the time that freezing tank temperature is below 0 ℃, open CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material;
The pressure that regulates separating tank 1 and separating tank 2 is 25-30MPa until pressure is the preferred pressure of 20-35MPa() time, carry out cycling extraction, and regulate CO
2flow be that 10-35kg/h(is preferably 25-35kg/h), keep constant temperature and pressure 1-3.5h(to be preferably 2-3h);
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
The present invention also provides the purposes of above-mentioned pine-seed oil, and it can be for the preparation of the healthcare products for delaying senility or medicine.
Wherein healthcare products or medicine can be made into oral preparations, comprise the formulations such as capsule, tablet, granule, oral liquid, paste, pill.These formulations can be according to well known to a person skilled in the art method preparation.
Healthcare products can add other pharmaceutical excipient in the time making preparation, for example: thinner, tackiness agent, correctives, solubilizing agent, disintegrating agent, lubricant, tinting material etc.
Experimentation on animals shows, pine-seed oil of the present invention can obviously improve the superoxide-dismutase (SOD) of body, reduces the lipid peroxide degraded product (MDA) of body, has the effect delaying senility.
The healthcare products or the medicine that prepare with pinenut oil of the present invention can be used for delaying senility.
Accompanying drawing explanation
Fig. 1 supercritical CO
2extraction plant schematic diagram
Wherein 1.CO
2steel cylinder, 2. cleaner 1,3. ice chest, 4. high pressure frequency modulation ram pump, 5. preheater, 6. cleaner 2,7. extractor, 8. separating tank 1,9. separating tank 2,10. under meter.
Embodiment
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 30-60 ℃ (preferred temperature is 35-45 ℃);
When freezing tank, (freezing tank is at CO
2before steel cylinder) temperature is below 0 ℃ time, opens CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material (Semen pini koraiensis);
The pressure that regulates separating tank 1 and separating tank 2 is 25-30MPa until pressure is the preferred pressure of 20-35MPa() time, carry out cycling extraction, and regulate CO
2flow be that 10-35kg/h(is preferably 25-35kg/h), keep constant temperature and pressure 1-3.5h(to be preferably 2-3h);
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
Embodiment 1
Get Semen pini koraiensis 60g in extractor, extraction temperature is set as 60 ℃, and extracting pressure is 35MPa, and flow control, at 35kg/h, is reacted 3.5 hours, obtains pine-seed oil 8.7g, and 10mg pine-seed oil/grain is made soft capsule.
Get Semen pini koraiensis 60g in extractor, extraction temperature is set as 30 ℃, and extracting pressure is 20MPa, and flow control, at 10kg/h, is reacted 1 hour, obtains pine-seed oil 7.5g, and 10mg pine-seed oil/grain is made soft capsule.
Get Semen pini koraiensis 60g in extractor, extraction temperature is set as 35 ℃, and extracting pressure is 25MPa, and flow control, at 25kg/h, is reacted 2 hours, obtains pine-seed oil 10.0g, and 10mg pine-seed oil/grain is made soft capsule.
Embodiment 4
Get Semen pini koraiensis 60g in extractor, extraction temperature is set as 45 ℃, and extracting pressure is 30MPa, and flow control, at 35kg/h, is reacted 3 hours, obtains pine-seed oil 10.8g, and 10mg pine-seed oil/grain is made soft capsule.
Embodiment 5
Test method: by (male 50 of Wistar rats (purchased from Chinese Academy of Sciences's Shanghai Experimental Animal Center), about 20 monthly ages, about 410-450g) be divided at random five groups (concrete clustering method in tables 1), adopt gastric infusion, successive administration 30 days, within 31 days, extract eyeball and get blood, get the high speed centrifugation that turns with per minute 3600 after blood 3 minutes, serum deprivation, with mda, superoxide-dismutase (SOD) testing cassete (Bioengineering Research Institute is built up in Nanjing) is measured the vigor of SOD and the content of lipid peroxide degraded product (MDA) in serum, bulk testing is tested by one-way analysis of variance method.
Table 1 clustering method:
Table 2 each group rat blood serum SOD vigor and the comparison of MDA content
* represents P < 0.01 compared with blank group.
High dose group, middle dosage group and low dose group rat blood serum SOD vigor have significant rising effect compared with blank group, though there is no significant difference compared with positive controls, high dose group has the trend that is better than positive controls; High dose group, middle dosage group and low dose group rat blood serum MDA content have significant reduction compared with blank group, and high dosage and positive controls approach.Pinenut oil has certain anti-aging effects as can be seen here.
Claims (5)
1. a pine-seed oil, is characterized in that it prepares by the following method:
Pine nut is carried out to supercritical CO
2extraction obtains.
2. pine-seed oil according to claim 1, is characterized in that it prepares by the following method:
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 30-60 ℃;
In the time that freezing tank temperature is below 0 ℃, open CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material;
Regulate the pressure of separating tank 1 and separating tank 2, until pressure while being 20-35MPa, carrying out cycling extraction, and regulates CO
2flow be 10-35kg/h, keep constant temperature and pressure 1-3.5h;
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
3. pine-seed oil according to claim 2, is characterized in that it prepares by the following method:
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 35-45 ℃;
In the time that freezing tank temperature is below 0 ℃, open CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material;
Regulate the pressure of separating tank 1 and separating tank 2, until pressure while being 25-30MPa, carrying out cycling extraction, and regulates CO
2flow be 25-35kg/h, keep constant temperature and pressure 2-3h;
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
4. a method of preparing pine-seed oil described in claim 1-3 any one, is characterized in that the method is:
Take Semen pini koraiensis and drop in extractor, screw extractor upper cover;
Open heater switch, respectively extractor, separating tank 1 and separating tank 2 are heated, Heating temperature is 30-60 ℃;
In the time that freezing tank temperature is below 0 ℃, open CO
2valve, CO
2gas enters ice chest liquefaction after cleaner 1 purifies, and sends into preheater preheating by high pressure frequency modulation ram pump, after cleaner 2 purifies, contacts with material;
Regulate the pressure of separating tank 1 and separating tank 2, until pressure while being 20-35MPa, carrying out cycling extraction, and regulates CO
2flow be 10-35kg/h, keep constant temperature and pressure 1-3.5h;
Extraction finishes, and from the discharge port discharging respectively of separating tank 1 and separating tank 2, obtains pine-seed oil.
5. the purposes of pine-seed oil described in claim 1-3 any one, it can be for the preparation of the healthcare products for delaying senility or medicine.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410053790.XA CN103789084A (en) | 2014-02-18 | 2014-02-18 | Pine-seed oil and preparation method and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410053790.XA CN103789084A (en) | 2014-02-18 | 2014-02-18 | Pine-seed oil and preparation method and use thereof |
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| CN103789084A true CN103789084A (en) | 2014-05-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410053790.XA Pending CN103789084A (en) | 2014-02-18 | 2014-02-18 | Pine-seed oil and preparation method and use thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929144A (en) * | 2015-12-29 | 2017-07-07 | 辽阳帝昊农业有限公司 | A kind of hazelnut oil preparation method and equipment |
| CN110613787A (en) * | 2019-10-29 | 2019-12-27 | 张少清 | Vegetable oil composition and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465682A (en) * | 2002-06-24 | 2004-01-07 | 东北林业大学 | Working method of supercritical carbon dioxide extracting pine-seed oil |
| CN1477181A (en) * | 2003-07-18 | 2004-02-25 | 孙传经 | Method for extracting walnut oil and pine oil by using supercritical CO2 reverse extrastion process |
| CN101161113A (en) * | 2007-10-29 | 2008-04-16 | 大连工业大学 | Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method |
-
2014
- 2014-02-18 CN CN201410053790.XA patent/CN103789084A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465682A (en) * | 2002-06-24 | 2004-01-07 | 东北林业大学 | Working method of supercritical carbon dioxide extracting pine-seed oil |
| CN1477181A (en) * | 2003-07-18 | 2004-02-25 | 孙传经 | Method for extracting walnut oil and pine oil by using supercritical CO2 reverse extrastion process |
| CN101161113A (en) * | 2007-10-29 | 2008-04-16 | 大连工业大学 | Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method |
Non-Patent Citations (1)
| Title |
|---|
| 胡小泓等: "超临界CO2 对整粒松仁萃取的研究", 《中国油脂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929144A (en) * | 2015-12-29 | 2017-07-07 | 辽阳帝昊农业有限公司 | A kind of hazelnut oil preparation method and equipment |
| CN110613787A (en) * | 2019-10-29 | 2019-12-27 | 张少清 | Vegetable oil composition and preparation method and application thereof |
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Application publication date: 20140514 |