CN106994131B - A kind of application adjusting lipid metaboli and fat compound PAQG in pharmacy - Google Patents
A kind of application adjusting lipid metaboli and fat compound PAQG in pharmacy Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A kind of 3 β-O- β-D-quinovopyranosyl-28-O- β-D-glucopyranoside (PAQG) of drop oleanolic acid compound Pyrocincholic Acid being extracted from yellow Populus deltoides new function and its application in the drugs, food and health care product that preparation improves fat and fat correlated metabolism diseases for regulating and controlling lipid metaboli in 3T3-L1 cell are provided.PAQG inhibits the differentiation of 3T3-L1 PECTORAL LIMB SKELETON by activation AMPK, lowers the expression of differentiation associated transcription factor and albumen (PPAR γ, C/EBP β, C/EBP α and FABP4) and the release of adiponectin.Meanwhile also by inhibiting fatty acid synthesis and lipid oxidation being promoted to inhibit the accumulation of fat in 3T3-L1 mature fat cell.Under safely and effectively dosage, PAQG improves obesity in terms of adipocyte count and fat content size two, can be used for preparing drug, health care product and the functional food of prevention and treatment obesity and fat relevant metabolic disease.
Description
Technical field:
The invention belongs to technical field of pharmaceuticals, and in particular to a kind of drop oleanolic acid compound Pyrocincholic Acid
3 β-O- β-D-quinovopyranosyl-28-O- β-D-glucopyranoside (PAQG) regulate and control rouge in 3T3-L1 cell
The new function of metabolism and its in the drugs, food and health care product that preparation improves fat and fat correlated metabolism diseases
Using.
Technical background:
Diabetes seriously jeopardize the health of the mankind as a kind of chronic metabolic disease, its complication.Especially
Be the diabetes B closely related with human life style research it is also more and more.Studies have shown that 50% obese people is all
With diabetes B, and the fat illness rate that can make diabetes B increases by 3 times.So the fat research with diabetes
Also it is increasingly concerned.
Obesity is clearly defined as " excessive or abnormal accumulation of fat in vivo " by the World Health Organization (WHO), and causes body
From the aspect of the content two that the reason of interior accumulation of fat can be fatty from adipocyte count and fat cell.To accumulation of fat
Research also become one treatment diabetes novel targets.Although improving fat drug there is various on the market,
Mostly since drug effect is low, side effect is big and unsalable, so, study a kind of medicine for safely and effectively improving fat treatment diabetes
Object or compound are still extremely necessary.
PAQG is a kind of novel drop oleanolic acid type saponin small molecule, is extracted from yellow Populus deltoides.Yellow Populus deltoides is Rubiaceae
Single platymiscium in yellow Populus deltoides category, is grown on southwest China, Vietnam, the ground such as India extensively.Currently, this kind of compounds are only
More than 20 kinds are isolated, PAQG is exactly one such.Moreover, the activity research of these compounds is also fewer.It is of the invention first
It is secondary to have found PAQG in 3T3-L1 cell to the regulating and controlling effect of lipid metaboli.
Summary of the invention:
The purpose of the present invention is to provide a kind of with the compound for significantly inhibiting 3T3-L1 cell differentiation and accumulation of fat
PAQG and the compound are in terms of preparation improves the drug, food and health care product of fat and fat correlated metabolism diseases
Using.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
Application of the compound PAQG in the inhibitor that 3T3-L1 PECTORAL LIMB SKELETON is broken up in preparation,
Compound PAQG is preparing the application in the regulator to mature fat cell fat metabolism.
Compound PAQG is preparing the application in slimming medicine.
Application of the compound PAQG in the drug that preparation adjusts blood lipid.
Application in slimming medicine is being prepared and prepared by the compound PAQG according to the compound PAQG
The application in the drug of blood lipid is adjusted, wherein PAQG is to lower differentiation phase by significantly inhibiting the differentiation of 3T3-L1 PECTORAL LIMB SKELETON
The expression of transcription factor and albumen PPAR γ, C/EBP β, C/EBP α and FABP4 and the release of adiponectin are closed, and passes through inhibition
Fatty acid synthesizes and promotes lipid oxidation to inhibit the accumulation of fat in fat cell, reaches weight-reducing and regulating blood lipid action.
The present invention additionally provides compound PAQG simultaneously improves the fat correlated metabolism of fat and treatment or prevention in preparation
Application in the drug of disease.
Improve medicine that is fat and treating or preventing fat correlated metabolism diseases in preparation according to the compound PAQG
Application in object, wherein PAQG be by significantly inhibit 3T3-L1 PECTORAL LIMB SKELETON differentiation, lower differentiation associated transcription factor and
The expression of albumen PPAR γ, C/EBP β, C/EBP α and FABP4 and the release of adiponectin, and by inhibit fatty acid synthesis and
Promote lipid oxidation to inhibit the accumulation of fat in fat cell, reaches antiobesity action.
The present invention, which still further provides compound PAQG, improves the food of fat and fat correlated metabolism diseases in preparation
And the application in health care product.
By a large amount of multiple independent repeated trials, have found PAQG in 3T3-L1 cell to the regulation of lipid metaboli for the first time
Effect.Present invention discover that PAQG can significantly inhibit the differentiation of 3T3-L1 PECTORAL LIMB SKELETON, differentiation associated transcription factor and egg are lowered
The expression of white (PPAR γ, C/EBP β, C/EBP α and FABP4) and the release of adiponectin.Meanwhile by inhibiting fatty acid synthesis
Inhibit the accumulation of fat in fat cell with lipid oxidation is promoted.Its effect for adjusting lipid metaboli may be with AMPK signal path
It activates related.Under safely and effectively dosage, PAQG improves in terms of adipocyte count and fat content size two
It is fat.
Detailed description of the invention
Fig. 1 oil red O stain.The induction of 3T3-L1 PECTORAL LIMB SKELETON differentiation 6 days.The PAQG of experimental group addition various concentration；
Compound is not added in con group, is substituted with 0.1%DMSO；Undifferentiated group does not add induction agent as blank control.
Experiment is independent in triplicate.
The induction of Fig. 2 .3T3-L1 PECTORAL LIMB SKELETON differentiation 6 days, experimental group added the PAQG of various concentration, being not added of control group
Object is closed, is substituted with 0.1%DMSO.(A) break up associated transcription factor (PPAR γ, C/EBP β and C/EBP α) and albumen FABP4
Electrophoresis result；(B) the adiponectin emission levels in culture medium.Experiment is independent in triplicate, and comparison among groups use independent sample t
It examines, error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
The fat cell that Fig. 3 differentiation is completed, is handled 48 hours, 0.1%DMSO is compared with various concentration PAQG.(A),
Upper figure, medication；The following figure, after administration 48 hours, fat drips content in fat cell；(B), under normal circumstances, 3T3-L1 fat
The time-concentration curve of triglycerides in cell；(C), after drug treatment, in 3T3-L1 fat cell triglycerides when
M- concentration curve；(D), after drug treatment, the time-concentration curve of the free fatty acid in 3T3-L1 fat cell；(E), it gives
After medicine processing, the time-concentration curve of the glycerol in 3T3-L1 fat cell；(F), glycerol release in 3T3-L1 fat cell
Time-concentration curve.Experiment is independent in triplicate, and comparison among groups use independent samples t test, and error line is standard error.With it is right
Compare according to group, P < 0.01 * P < 0.05, * *.
Fig. 4 (A), 3T3-L1 PECTORAL LIMB SKELETON break up in the inductive differentiation medium containing various concentration PAQG, and 6 days
Afterwards, the expression of the albumin A MPK and p-AMPK that are detected with polyacrylamide gel electrophoresis；(B), to the amount of (A) experimental result
Change；(C), 20 μM of PAQG, the DMSO of control group addition 0.1% is added in the overall process of 3T3-L1 PECTORAL LIMB SKELETON induction differentiation,
Then the expression of collection albumen sample detection AMPK and p-AMPK is checked and accepted in different times；(D), to (C) experimental result
Quantization (arrow indicates that compound PAQG starts drug treatment).Experiment is independent in triplicate, and comparison among groups are examined using independent sample t
It tests, error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
Fig. 5 by the PAQG of the 3T3-L1 fat cell various concentration broken up be incubated for 48 hours (collect protein sample and
RT-PCR sample).(A), the electrophoresis result of p-AMPK and AMPK；(B), to the quantization of (A) protein electrophoresis result；(C), RT-PCR
As a result: by the expression of the AMPK gene (SREBP1c, ACC α, SCD1, FAS) relevant to fatty acid synthesis regulated and controled；(D), RT-
PCR result: by the expression of the AMPK gene (PDK4, CPT1a, ACOX1) relevant to lipid oxidation regulated and controled.Experiment is independent to repeat three
Secondary, comparison among groups use independent samples t test, and error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
Fig. 6 is in 3T3-L1 cell, the cytotoxicity experiment of compound PAQG.Experiment is independent in triplicate.
Specific embodiment:
With reference to the accompanying drawing, essentiality content of the invention is further illustrated with the embodiment of the present invention, but not with
This limits the present invention.
Embodiment 1:
The compounds of this invention PAQG is extracted from yellow Populus deltoides shown in structural formula such as following formula (I), by the double versions in Chinese Academy of Sciences west
Receive tropical plants garden offer, preparation method please refers to documented method in following document.
ZHANG Y,TAN N,HUANG H,et al.Triterpenoid Saponins from Metadina
trichotoma[J].Zeitschrift für Naturforschung B,2007,62(5):745‐748.
1, PAQG inhibits the differentiation of 3T3-L1 PECTORAL LIMB SKELETON:
3T3-L1 PECTORAL LIMB SKELETON is two days hungry, with induction agent induction differentiation three days, then with maintenance culture medium culture one
It, replacing fresh culture later and normally cultivating is to be divided into fat cell.According to the experimental method, in entire atomization
It is handled with the PAQG of various concentration (0,2.5 μM, 5 μM, 10 μM, 20 μM), control group is substituted with 0.1% DMSO.It is complete to cell
After full differentiation is good, with the fat content in the method detection fat cell of oil red O stain.As shown in Figure 1, pair with normal differentiation
It is compared according to a group cell, the differentiation of administration group fat cell is obviously inhibited, and concentration gradient dependence is presented, and 20 μM
PAQG is up to 90% to the suppression level of Adipocyte Differentiation, does not observe fat drips substantially in cell at this time.
Fig. 1 oil red O stain.The induction of 3T3-L1 PECTORAL LIMB SKELETON differentiation 6 days.The PAQG of experimental group addition various concentration；
Compound is not added in con group, is substituted with 0.1%DMSO；Undifferentiated group does not add induction agent as blank control.
Experiment is independent in triplicate.
The induction of Fig. 2 .3T3-L1 PECTORAL LIMB SKELETON differentiation 6 days, experimental group added the PAQG of various concentration, being not added of control group
Object is closed, is substituted with 0.1%DMSO.(A) break up associated transcription factor (PPAR γ, C/EBP β and C/EBP α) and albumen FABP4
Electrophoresis result；(B) the adiponectin emission levels in culture medium.Experiment is independent in triplicate, and comparison among groups use independent sample t
It examines, error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
Meanwhile the present invention collects the above protein sample tested, and is detected with agarose gel electrophoresis method, discovery differentiation is related
The expression of transcription factor and albumen (PPAR γ, C/EBP β, C/EBP α and FABP4) is remarkably decreased (Fig. 2A), also, uses adiponectin
Kit detects that the release of adiponectin in culture medium also reduces as shown in Figure 2 B.Result in terms of three above proves PAQG
Significantly inhibit the differentiation of 3T3-L1 PECTORAL LIMB SKELETON.
2, PAQG inhibits the accumulation of fat in mature fat cell
After 3T3-L1 PECTORAL LIMB SKELETON normal differentiation, handled 48 hours with 10 μM and 20 μM of PAQG respectively, control group
For 0.1%DMSO (such as on Fig. 3 A).Observation result is as shown in Figure 3A under the microscope, and compared with cellular control unit, experimental group is thin
Rouge content in born of the same parents significantly reduces.Meanwhile we dissolve with dehydrated alcohol and collect the rouge in cell, and detect wherein glycerol three
The content of ester (TG).As shown in Figure 3B, consistent with microscopic examination result, TG content significant decrease intracellular after PAQG processing, in concentration ladder
Spend dependence.Cell without doing drug treatment, after normal differentiation, TG intracellular is (Fig. 3 B) constantly accumulated.PAQG causes
TG is reduced in fat cell, and the TG hydrolysis in fat cell may be promoted due to it, generates free fatty acid (FFA) and glycerol
(Glycerol), it is discharged into culture medium.Therefore, we have detected the content of FFA and Glycerol in culture medium with kit, such as
Shown in Fig. 3 D and 3E, FFA the and Glycerol content in culture medium is all dramatically increased (compared with Normal group), and is also in
Concentration gradient dependence.
For the fat content variation in dynamic detection PAQG treatment process, in fat cell.We are in PAQG processing
In 48 hours, the glycerol content (Fig. 3 F) in different time points culture medium is detected, and bent with the time that this depicts glycerol release
Line.The results show that in control group and experimental group, the content of glycerol is all to gradually increase as time went on, but chemical combination
After object processing, the content of glycerol is apparently higher than control group, and is in concentration gradient dependence.Result prompt, the lipid-loweringing of PAQG
Effect is related with TG degradation in fat cell is promoted.
The fat cell that Fig. 3 differentiation is completed, is handled 48 hours, 0.1%DMSO is compared with various concentration PAQG.(A),
Upper figure, medication；The following figure, after administration 48 hours, fat drips content in fat cell；(B), under normal circumstances, 3T3-L1 fat
The time-concentration curve of triglycerides in cell；(C), after drug treatment, in 3T3-L1 fat cell triglycerides when
M- concentration curve；(D), after drug treatment, the time-concentration curve of the free fatty acid in 3T3-L1 fat cell；(E), it gives
After medicine processing, the time-concentration curve of the glycerol in 3T3-L1 fat cell；(F), glycerol release in 3T3-L1 fat cell
Time-concentration curve.Experiment is independent in triplicate, and comparison among groups use independent samples t test, and error line is standard error.With it is right
Compare according to group, P < 0.01 * P < 0.05, * *.
3, PAQG activates AMPK signal path in 3T3-L1 cell
When observing that PAQG inhibits 3T3-L1 PECTORAL LIMB SKELETON differentiating phenomenon, we collect the protein sample, and
With the protein expression of agarose gel electrophoresis method detection AMPK and p-AMPK.As illustrated in figures 4 a and 4b, the phosphorylation level of AMPK
Increase as gradient is presented in the concentration variation of PAQG.In order to further probe into AMPK in 3T3-L1 PECTORAL LIMB SKELETON atomization
The situation of change of phosphorylation, We conducted following experiments.Administration when inducing differentiation since just, control group is with 0.1%
DMSO substitution, in day1, day3, day4, day6, these different times check and accept albumen, detect the expression of AMPK and p-AMPK.
As a result as shown in figures 4 c and 4d, PAQG can sustained activation AMPK during the entire process of 3T3-L1 PECTORAL LIMB SKELETON breaks up
Phosphorylation.
In order to probe into the mechanism of action that PAQG inhibits the accumulation of 3T3-L1 cellular fat, We conducted following experiments: when thin
After born of the same parents break up, cell is handled with 10 μM and 20 μM of PAQG respectively, uses 0.1%DMSO as control.Two days later, a part of
Collect the expression of protein sample detection AMPK and p-AMPK；Another part collect RNA sample detection AMPK signal path downstream with
The relevant gene expression of lipid metaboli.Wherein, AMPK signal path gene relevant to lipid metaboli mainly include fatty acid synthesis and
Two class of lipid oxidation.And gene relevant to fatty acid synthesis mainly has: SREBP1c, ACC α, SCD1, FAS；It is related to lipid oxidation
Gene mainly have: PDK4, CPT1a, ACOX1.As shown in figures 5 a-d, the phosphorylation level of AMPK is with PAQG's for experimental result
Concentration variation is presented gradient and increases.In addition, the expression of gene SREBP1c, ACC α, SCD1, FAS reduce, and PDK4 and CPT1a
Expression increase, the expression of ACOX1 is basically unchanged.Thus illustrate, PAQG may inhibit fat by activating AMPK signal path
Acid synthesizes and promotes lipid oxidation, to play effect for reducing fat.
Fig. 4 .A), 3T3-L1 PECTORAL LIMB SKELETON breaks up in the inductive differentiation medium containing various concentration PAQG, and 6 days
Afterwards, the expression of the albumin A MPK and p-AMPK that are detected with polyacrylamide gel electrophoresis；(B), to the amount of (A) experimental result
Change；(C), 20 μM of PAQG, the DMSO of control group addition 0.1% is added in the overall process of 3T3-L1 PECTORAL LIMB SKELETON induction differentiation,
Then the expression of collection albumen sample detection AMPK and p-AMPK is checked and accepted in different times；(D), to (C) experimental result
Quantization (arrow indicates that compound PAQG starts drug treatment).Experiment is independent in triplicate, and comparison among groups are examined using independent sample t
It tests, error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
Fig. 5 by the PAQG of the 3T3-L1 fat cell various concentration broken up be incubated for 48 hours (collect protein sample and
RT-PCR sample).(A), the electrophoresis result of p-AMPK and AMPK；(B), to the quantization of (A) protein electrophoresis result；(C), RT-PCR
As a result: by the expression of the AMPK gene (SREBP1c, ACC α, SCD1, FAS) relevant to fatty acid synthesis regulated and controled；(D), RT-
PCR result: by the expression of the AMPK gene (PDK4, CPT1a, ACOX1) relevant to lipid oxidation regulated and controled.Experiment is independent to repeat three
Secondary, comparison among groups use independent samples t test, and error line is standard error.Compared with the control group, P < 0.01 * P < 0.05, * *.
4, the cytotoxicity experiment of PAQG
The present invention has detected cytotoxicity experiment (Fig. 6) of the PAQG in mouse 3T3-L1 PECTORAL LIMB SKELETON.The result shows that
For compound PAQG without cytotoxicity within the scope of 0~50 μM, the compound maximum concentration used in this experimentation is 20 μM,
This concentration is in safe range.
Fig. 6 is in 3T3-L1 cell, the cytotoxicity experiment of compound PAQG.Experiment is independent in triplicate.
Embodiment 2
By 1 gained compound PAQG of embodiment, figuration is added in the ratio of compound and excipient weight ratio 1:1 or 1:2
Agent, pelletizing press sheet.
Embodiment 3:
Compound PAQG is made by embodiment 1, routinely capsule is made in capsule preparations method.
Embodiment 4:
Compound PAQG first is made by embodiment 1, then tablet is made as follows.
Tablet: compound PAQG100mg, appropriate starch, appropriate corn pulp, Magnesium Stearate proper quantity.Preparation method: by chemical combination
Object is mixed with auxiliary agent, and sieving uniformly mixes in suitable container, obtained granulating mixture tabletting.
Embodiment 5:
Capsule: compound PAQG100mg, appropriate starch, Magnesium Stearate proper quantity.
Preparation method: compound PAQG is mixed with auxiliary agent, and sieving uniformly mixes in suitable container, what is obtained
Mixture is packed into hard gelatin capsule.
Embodiment 6:
Tablet: embodiment 1 gained compound PAQG10mg, lactose 180mg, starch 55mg, magnesium stearate 5mg；
Preparation method: compound PAQG, newborn sugar and starch are mixed, uniformly moistened with water, the mixture mistake after moistening
It sieves and dries, magnesium stearate is added, then by mixture tabletting, every slice weight 250mg, compounds content 10mg in re-sieving.
Embodiment 7:
Ampulla: embodiment 1 gained compound PAQG2mg, sodium chloride 10mg；
Preparation method: compound PAQG and sodium chloride are dissolved in suitable water for injection, acquired solution are filtered, in nothing
It is fitted into ampoule bottle under the conditions of bacterium.
Embodiment 8:
Capsule: embodiment 1 gained compound PAQG10mg, lactose 187mg, magnesium stearate 3mg；
Preparation method: compound is mixed with auxiliary agent, and sieving uniformly mixes, and obtained mixture is packed into glutoid glue
Capsule, each capsule weight 200mg, active component content 10mg.
Embodiment 9:
The preparation of food:
Preparation method: 1 gained compound PAQG of Example is added in jam with the ratio of 1:7, can increase food
Fragrance, while can also play health-care efficacy.
Embodiment 10:
The preparation of food:
Preparation method: 1 gained compound PAQG of Example is added in honey with the ratio of 1:8, can increase food
Fragrance, while can also play health-care efficacy.
Embodiment 11:
Sour milk beverage formula (W%) containing compound PAQG:
Above-mentioned beverage of the invention is made in the method for routinely making food.
Claims (6)
1. compound PAQG is preparing the application in slimming medicine, shown in the compound PAQG structural formula such as formula (I),
2. application of the compound PAQG in the drug that preparation adjusts blood lipid, the compound PAQG structural formula such as formula (I) institute
Show,
3. compound PAQG according to claim 1 is preparing the application in slimming medicine, it is characterised in that PAQG passes through
Significantly inhibit 3T3-L1 PECTORAL LIMB SKELETON differentiation, lower differentiation associated transcription factor and albumen PPAR γ, C/EBP β, C/EBP α and
The expression of FABP4 and the release of adiponectin, and inhibited in fat cell by inhibiting fatty acid synthesis and promoting lipid oxidation
Accumulation of fat, reach antiobesity action.
4. application of the compound PAQG according to claim 2 in the drug that preparation adjusts blood lipid, it is characterised in that
PAQG breaks up associated transcription factor and albumen PPAR γ, C/EBP β by significantly inhibiting the differentiation of 3T3-L1 PECTORAL LIMB SKELETON, downward,
The expression of C/EBP α and FABP4 and the release of adiponectin, and inhibit rouge by inhibiting fatty acid synthesis and promoting lipid oxidation
Accumulation of fat in fat cell, reaches regulating blood lipid action.
5. compound PAQG improves the application in drug that is fat and treating or preventing fat correlated metabolism diseases, institute in preparation
Shown in the compound PAQG structural formula stated such as formula (I),
6. compound PAQG according to claim 5 improves the fat correlated metabolism disease of fat and treatment or prevention in preparation
Application in the drug of disease, it is characterised in that PAQG is broken up by significantly inhibiting 3T3-L1 PECTORAL LIMB SKELETON, and it is related to lower differentiation
The expression of transcription factor and albumen PPAR γ, C/EBP β, C/EBP α and FABP4 and the release of adiponectin, and by inhibiting rouge
Fat acid synthesizes and promotes lipid oxidation to inhibit the accumulation of fat in fat cell, reaches antiobesity action.
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| CN108610409B (en) * | 2018-04-09 | 2021-08-24 | 深圳大学 | Application of ETV5 in preparation of medicine for preventing or treating obesity and related metabolic diseases |
| CN109010800A (en) * | 2018-07-05 | 2018-12-18 | 中国科学院深圳先进技术研究院 | PDGF-BB is inhibiting Adipocyte Differentiation, formation and its application of function |
| CN109722421B (en) * | 2018-12-14 | 2022-04-19 | 南京市妇幼保健院 | Adipocyte endogenous polypeptide and application thereof |
| CN110974821A (en) * | 2019-12-24 | 2020-04-10 | 浙江大学 | Application of Eurycomanone and analogues thereof in preparation of medicines for treating hyperlipidemia and fatty liver or weight-reducing products |
| CN111926071B (en) * | 2020-08-14 | 2022-01-04 | 武汉大学 | Molecular marker related to aging and healthy aging and application of molecular marker in improving healthy aging |
| CN117064903A (en) * | 2022-05-10 | 2023-11-17 | 上海中医药大学附属龙华医院 | Application of semen astragali Complanati glycoside A in preparation of medicine for preventing and treating obesity |
| CN114931577A (en) * | 2022-06-21 | 2022-08-23 | 上海市同仁医院 | Application of indole-3-acrylic acid in inhibiting differentiation of white adipose precursor cells |
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