CN102907677A - Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof - Google Patents
Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof Download PDFInfo
- Publication number
- CN102907677A CN102907677A CN2012104569065A CN201210456906A CN102907677A CN 102907677 A CN102907677 A CN 102907677A CN 2012104569065 A CN2012104569065 A CN 2012104569065A CN 201210456906 A CN201210456906 A CN 201210456906A CN 102907677 A CN102907677 A CN 102907677A
- Authority
- CN
- China
- Prior art keywords
- sea
- buckthorn
- seed oil
- soft capsule
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a preparation method of a health-care product having a function of assisting blood-fat reduction, in particular to a preparation method of a sea-buckthorn perilla seed oil soft capsule having a function of assisting the blood-fat reduction. The sea-buckthorn perilla seed oil soft capsule consists of a capsule casing and contents and is characterized in that the capsule casing consists of, by weight, edible gelatine 40%-45%, glycerol 18%-25% and pure water 35%-40%, and the contents include, by weight, sea-buckthorn seed oil 30%-50% and perilla seed oil 50%-70%. In addition, the preparation method comprises the steps of firstly adopting a supercritical CO2 fluid extraction technology to respectively extract the sea-buckthorn seed oil and the perilla seed oil, evenly mixing the sea-buckthorn seed oil and the perilla seed oil according to formula amount and manufacturing a mixture into soft capsules in a dropping-pill mode, wherein the sea-buckthorn perilla seed oil soft capsule does not contain solvents and other impurities and contains abundant unsaturated fatty acid, vitamin E and various nutritional health-care ingredients. A health-care food functional experiment and a toxicology safety evaluation test prove that the sea-buckthorn perilla seed oil soft capsule has the function of assisting the blood-fat reduction and is suitable for long-term taking of people with high blood fat.
Description
Technical field
The present invention relates to a kind of preparation method with auxiliary lipid-lowering function health food, be specifically related to the preparation method of sea-buckthorn perilla herb oil soft capsule.
Background technology
According to the incidence of disease increasing year by year of investigation hyperlipemia in China, the death rate of the cardiovascular and cerebrovascular disease that hyperlipemia directly causes has also accounted for first of various diseases.It should be noted that blood fat is light, moderate increases itself and do not cause clinical symptoms, cause high fat of blood atherosclerotic (AS) on the vascular wall but slowly be deposited on, allow narrow and fragile that blood vessel becomes, finally cause cardiovascular and cerebrovascular disease.There are data to show, it is the three to four-fold that has been diagnosed as Patients with Cardiovascular/Cerebrovascular Diseases that the reality of hyperlipemia has rate, and therefore, AS is one of important risk factor that occurs cardiovascular and cerebrovascular disease, it is generally acknowledged that reducing blood fat can slow down the formation of AS, thereby prevent cardiovascular and cerebrovascular disease.The fat-reducing medicament of using clinically at present has the toxic and side effect of liver, injury of kidney more, therefore, finds to have the raw material of fat-reducing effect from " medicine-food two-purpose " resource, and the exploitation side effect is little, the health food of stable effect, and can yet be regarded as solves effective ways of lipopenicillinase.
Find through nearly 30 years research, there is the Chinese herbal medicine of effect for reducing fat to have tens kinds, from effect, effect for reducing blood fat mainly all shows and reduces serum total cholesterol (TC), sea-buckthorn is exactly one of them, and the Chinese herbal medicine of triglyceride reducing (TG) effect is relatively less, and purple perilla is exactly wherein one of to represent.
Sea-buckthorn belongs to the Elaeangnaceae Hippophne, is a kind of fallen leaves shrub, and is drought-enduring, and anti-blown sand is widely used in water and soil conservation and plants.China is the medical history of sea-buckthorn country the earliest in the world, and sea-buckthorn early is used as the common drug for the treatment of various diseases by Tibetan medicine, Mongolian medicine.Before 1300, " month king medicine precious " of China's the Tang Dynasty, the Four-Volume Medical Code, and " Jingzhubencao " of the Qing Dynasty etc. all put down in writing the medical usage of sea-buckthorn.Sea-buckthorn is regarded as the plant of " integration of drinking and medicinal herbs " in the first batch by health ministry.Seabuckthorm Seed Oil extracts from seabuckthorn seeds and obtains; be rich in the multiple biological activities to the human body beneficial such as unrighted acid, vitamin E, phytosterol, Flavonoid substances and trace element, have the effects such as reducing blood lipid, hypotensive, immunological regulation, inhibition tumour, anti-inflammatory myogenic, treatment burn and scald, cardioprotection.Seabuckthorm Seed Oil reaches the purpose of reducing blood lipid by reducing serum total cholesterol (TC).
Purple perilla is first batch of " integration of drinking and medicinal herbs " plant assert of the traditional drug-food plant of China and health ministry equally, have reducing blood lipid and hypotensive, suppress platelet aggregation, reduce thrombosis, the prevention canceration, improve the effects such as memory, antiallergy.Purple Perilla Seed Oil extracts from the purple perilla seed and obtains, contain equally abundant unrighted acid, Flavonoid substances, carotenoid material and trace element, Purple Perilla Seed Oil is mainly by substantial reduction in triglycerides (TG) concentration, increasing high density lipoprotein cholesterol (HDL-C), thus reach the purpose of reducing blood lipid.
Seabuckthorm Seed Oil, Purple Perilla Seed Oil all have preferably edible, medical value.On the basis of the researchs such as biochemical analysis, according to Seabuckthorm Seed Oil and Purple Perilla Seed Oil characteristic separately, scientific formula is proposed, Seabuckthorm Seed Oil and Purple Perilla Seed Oil are carried out the composite sea-buckthorn perilla herb oil that obtains.Make it mutually auxiliary composition performance complementation coordinate synergistic effect, both reduced serum total cholesterol (TC) concentration, substantial reduction in triglycerides (TG) concentration again, increasing high density lipoprotein cholesterol (HDL-C) concentration, thereby reach the purpose of compound reducing blood lipid, overcome the defective of the single lipopenicillinase of existing product.Seabuckthorm Seed Oil, Purple Perilla Seed Oil both all to contain abundant unrighted acid be its denominator, but unrighted acid is oxidation very easily, the content of vitamin E is up to 400mg/100g in the Seabuckthorm Seed Oil, needn't add antioxidant, antioxidation is remarkable, can avoid the easy oxidation of sea-buckthorn perilla herb oil, and the cost of Purple Perilla Seed Oil only has 1/20th of Seabuckthorm Seed Oil cost simultaneously, greatly reduce the cost of sea-buckthorn perilla herb oil, remarkable in economical benefits.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, sea-buckthorn perilla herb oil soft capsule of a kind of auxiliary lipid-lowering function and preparation method thereof is provided, to overcome the defective of the single lipopenicillinase of existing product.
For achieving the above object, the technical scheme that the present invention takes is: a kind of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, its main feature is that the percentage by weight of its softgel shell is: edible gelatin 40%～45%, glycerine 18%～25%, pure water 35%～40%, its content weight percentage is: Seabuckthorm Seed Oil 30～50%, Purple Perilla Seed Oil 70～50%.
The sea-buckthorn perilla herb oil soft capsule of described auxiliary lipid-lowering function, its softgel shell optimum weight percentage: edible gelatin 42%, glycerine 20%, pure water 38%.
The sea-buckthorn perilla herb oil soft capsule of described auxiliary lipid-lowering function, its content optimum weight percentage is: Seabuckthorm Seed Oil is 50%, Purple Perilla Seed Oil is 50%.
The sea-buckthorn perilla herb oil soft capsule of described auxiliary lipid-lowering function contains effective component: oleic acid 21.1g ± 10%g, linoleic acid 23.0g ± 10%g, leukotrienes 44.3g ± 20%g, natural VE 〉=0.12g in every 100g content.
A kind of preparation method of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, its main feature is the preparation method of Seabuckthorm Seed Oil, the steps include:
Behind seabuckthorn seeds raw material drying, pulverizing 40～60 orders, take by weighing the seabuckthorn seeds raw material, drop in the supercritical extraction reactor, close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 20～22Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 15～20kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 2h under these conditions, obtains the seabuckthorn seeds crude oil; The seabuckthorn seeds crude oil that extraction obtains separates through supercentrifuge, and centrifuge speed is 16000～18000n/min, obtains the transparent Seabuckthorm Seed Oil of brownish red.
A kind of preparation method of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, its main feature is the preparation method of Purple Perilla Seed Oil, the steps include:
Behind purple perilla seed raw material drying, pulverizing 40～60 orders, take by weighing the purple perilla seed raw material, drop in the supercritical extraction reactor, close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 22～24Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 80～100kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 6h under these conditions, obtains purple perilla seed crude oil; The purple perilla seed crude oil that extraction obtains is centrifugal through supercentrifuge, and centrifuge speed is 16000～18000n/min, obtains faint yellow transparent violet perilla oil.
A kind of preparation method of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, its main feature is the preparation method of sea-buckthorn perilla herb oil soft capsule, the steps include:
Add glycerine 18%～25% by claim 1 percentage by weight in glue pot, pure water 35%～40% adds edible gelatin 40%～45% after stirring and be heated to 75～80 ℃, stirs 60～90min, and gelatin is dissolved fully; Open vavuum pump, continue to stir 45～60min under 0.06～0.08MPa vacuum condition, carry out vacuumize degassing, stop after degassed the finishing stirring, close vavuum pump, glue leaves standstill cooling 4h; With emulsification in 30～50% Seabuckthorm Seed Oils and the composite adding emulsion tank of 70～50% Purple Perilla Seed Oils, stir; Sea-buckthorn perilla herb oil after the emulsification is evenly carried out pelleting, 18～24 ℃ of pelleting temperature; The capsule and pill of compacting enters and rolls cage typing drying, and rolling the cage rotating speed is 40～50r/min, shaping time 〉=4h, to enter basin natural drying from rolling cage after the capsule and pill typing, and 22～27 ℃ of indoor temperatures, relative humidity are≤50%, time is 30～36h, dry good sea-buckthorn perilla herb oil soft capsule alcohol disinfecting.
The beneficial effect that the present invention produces
The present invention selects sea-buckthorn and the purple perilla vegetable seeds of being regarded as " integration of drinking and medicinal herbs " by health ministry, adopts supercritical CO
2The fluid extraction new and high technology, extract respectively Seabuckthorm Seed Oil, Purple Perilla Seed Oil, all has preferably edible, medical value, on the basis of the researchs such as biochemical analysis, according to Seabuckthorm Seed Oil and Purple Perilla Seed Oil characteristics separately, learn from other's strong points to offset one's weaknesses, Seabuckthorm Seed Oil and Purple Perilla Seed Oil are carried out composite, the experiment proved that, join dissolubility good, mutual aid is strong, can the complementary coordination of mutually auxiliary composition performance synergistic effect.Sea-buckthorn perilla herb oil dripping pill is made soft capsule, wrap up, prevent that it is directly exposed in the air, Seabuckthorm Seed Oil and Purple Perilla Seed Oil high-load unrighted acid have effectively been completely cut off directly and the air catalytic oxidation, its stability improves greatly, has prolonged the shelf-life of product, has overcome again oral grease smell, easily accepted instant by the patient.Product is through China Preventive Medicial Science Institute's nutrition and the tested proof of health research: the health care with auxiliary antilipemic.
Description of drawings
Fig. 1 is supercritical CO of the present invention
2Fluid extraction Seabuckthorm Seed Oil production technology sketch;
Fig. 2 is supercritical CO of the present invention
2Fluid extraction Purple Perilla Seed Oil production technology sketch;
Fig. 3 is sea-buckthorn perilla herb oil soft capsule technological process of production sketch of the present invention.
The specific embodiment
Below in conjunction with accompanying drawing principle of the present invention and feature are described, institute gives an actual example and only is used for explaining the present invention, is not be used to limiting scope of the present invention.
Embodiment 1: a kind of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, the optimum weight percentage of its softgel shell: edible gelatin 42%, glycerine 20%, pure water 38%, its content weight percentage is: Seabuckthorm Seed Oil 30～50%, Purple Perilla Seed Oil 70～50%, the composite sea-buckthorn perilla herb oil that becomes.Contain effective component in every 100g content: oleic acid 21.1g ± 10%g, linoleic acid 23.0g ± 10%g, leukotrienes 44.3g ± 20%g, natural VE 〉=0.12g.Every soft capsule product contains content sea-buckthorn perilla herb oil 0.5g/ grain, and is oral to the Hyperlipidemia person, every day 2 times, each 4.
Embodiment 2: a kind of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, comprise its content weight percentage, and Seabuckthorm Seed Oil is 30%, Purple Perilla Seed Oil is 70%.
Embodiment 3: a kind of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, comprise its content weight percentage, and Seabuckthorm Seed Oil is 40%, Purple Perilla Seed Oil is 60%.
Embodiment 4.: a kind of sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function, comprise its content weight percentage, and Seabuckthorm Seed Oil is 50%, Purple Perilla Seed Oil is 50%.
Embodiment 5.: a kind of sea-buckthorn perilla herb oil soft capsule preparation method of auxiliary lipid-lowering function, the preparation method of Seabuckthorm Seed Oil sees Fig. 1, the steps include:
Behind seabuckthorn seeds raw material drying, pulverizing 40～60 orders, take by weighing 10kg, drop in the supercritical extraction reactor (24L), close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 20～22Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 15～20kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 2h under these conditions, obtains seabuckthorn seeds crude oil 600g, and yield is 6.0%; The seabuckthorn seeds crude oil that extraction obtains is removed water, free fatty and the impurity such as colloid, phospholipid substance through the supercentrifuge centrifugation, and centrifuge speed is 16000～18000n/min, finally obtains the transparent Seabuckthorm Seed Oil 550g of brownish red, and yield is 5.5%.
Embodiment 6: a kind of sea-buckthorn perilla herb oil soft capsule preparation method of auxiliary lipid-lowering function, the preparation method of Purple Perilla Seed Oil sees Fig. 2, the steps include:
Behind purple perilla seed raw material drying, pulverizing 40～60 orders, take by weighing 10kg, drop in the supercritical extraction reactor (24L), close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 22～24Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 80～100kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 6h under these conditions, obtains purple perilla seed crude oil 3800g, and yield is 38%; The purple perilla seed crude oil that extraction obtains is removed water, free fatty and the impurity such as colloid, phospholipid substance through the supercentrifuge centrifugation, and centrifuge speed is 16000～18000n/min, finally obtains faint yellow transparent violet perilla oil 3500g, and yield is 35%.
Embodiment 7: a kind of sea-buckthorn perilla herb oil soft capsule preparation method of auxiliary lipid-lowering function, the preparation method of sea-buckthorn perilla herb oil soft capsule sees Fig. 3, the steps include:
In glue pot, first add by weight percentage glycerine 20% and pure water 38%, add edible gelatin 42% after stirring and be heated to 75～80 ℃, stir 60～90min, gelatin is dissolved fully; Open vavuum pump, continue to stir 45～60min under 0.06～0.08MPa vacuum condition, carry out vacuumize degassing, stop after degassed the finishing stirring, close vavuum pump, glue leaves standstill cooling 4h; With emulsification in 30% Seabuckthorm Seed Oil and the composite adding emulsion tank of 70% Purple Perilla Seed Oil, stir; Sea-buckthorn perilla oil after the emulsification is evenly carried out pelleting, 18～24 ℃ of pelleting temperature; The capsule and pill of compacting enters and rolls cage typing drying, rolling the cage rotating speed is 40～50r/min, shaping time 〉=4h, to enter basin natural drying from rolling cage after the capsule and pill typing, 22～27 ℃ of indoor temperatures, relative humidity are≤50%, and the time is 30～36h, behind the dry good sea-buckthorn perilla herb oil soft capsule alcohol disinfecting, be finished product.
Experimental example 1:
The inventor entrusts China Preventive Medicial Science Institute's nutrition and health research institute function of health food inspection center, according to " function of health food assessment process and the method for inspection ", sea-buckthorn perilla herb oil soft capsule has been carried out the Evaluation of Functional test.
1, animal used as test
50 of secondary Wistar male rats, body weight 160-200g.
2, experimental technique
Feed rat after 5 days with basal feed, fasting is weighed, and gets tail blood, enzymatic assays serum total cholesterol (TC), triglycerides (TG), HDL-C (HDL-C).Animal is divided into 5 groups at random, is respectively:
Water control group: 0.0g/kgbw
Common ready-mixed oil control group: 3.0g/kgbw
Low dosage experimental group: 1.0g/kgbw
Middle dosage experiments group: 2.0g/kgbw
High dose experimental group: 3.0g/kgbw
From formal experiment, each treated animal is used high lipid food (proportioning: basal feed 93.3%, cholesterol 1.5%, lard 5%, cholate 0.2%) instead and is fed.Give animal subject in the gavage mode, the water control group gives distilled water in the gavage mode, and oily control group gives the common ready-mixed oil that censorship unit provides in the gavage mode, and each experimental group gives sea-buckthorn perilla herb oil soft capsule content in the gavage mode.28 days observation cycles, got before the blood fasting 12 hours at every turn, every day gavage once, weigh in the time of the 14th, 28 day, get the every blood lipids index of tail hematometry.
3, experimental data statistics
Check the significant difference of each experimental group and control group indices with the t-method of inspection.
4, experimental result
(1) sea-buckthorn perilla herb oil soft capsule is on the impact of rat body weight
Five groups of Growth in Rats of whole experiment periods (28 days) are normal.Each experimental group rat body weight is compared with oily control group without significant difference (p＞0.05) with the water control group.
(2) sea-buckthorn perilla herb oil soft capsule is to the Serum TC concentration affects
Measure in the time of the 28th day in experiment, middle dosage experiments group and high dose experimental group Serum TC concentration significantly are lower than respectively the water control group, the t value is respectively 3.604,2.346＞t0.05.18=2.101, significantly be lower than respectively oily control group, the t value is respectively 3.540,2.694＞t0.05.18=2.101, and difference (p＜0.05) is statistically arranged.
(3) sea-buckthorn perilla herb oil soft capsule is to rat blood serum TG concentration affects
Measured in the 14th day in experiment, high dose experimental group rat blood serum TG concentration significantly is lower than oily control group, the t value is 3.241＞t0.05.18=2.101, measured in the 28th day in experiment, middle dosage experiments group and high dose experimental group rat blood serum TG concentration significantly are lower than respectively the water control group, and the t value is respectively 3.549,3.729＞t0.05.18=2.101, significantly is lower than respectively oily control group, the t value is respectively 3.991,4.267＞t0.05.18=2.101, and difference (p＜0.05) is statistically arranged.
(4) sea-buckthorn perilla herb oil soft capsule is to rat blood serum HDL-C concentration affects
The HDL-C concentration of three experimental group is compared equal nothing difference (p＞0.05) statistically with the water control group with oily control group.
(5) sea-buckthorn perilla herb oil soft capsule is on the impact of rat blood serum HDL-C/TC ratio.
The ratio of cardiovascular " the protection factor " HDL-C in TC that HDL-C/TC ratio has reflected; measured in the 28th day in experiment; basic, normal, high dosage group rat blood serum HDL-C/TC ratio is significantly higher than oily control group; the t value is 2.126,2.589,2.383＞t0.05.18=2.101, and difference (p＜0.05) is statistically arranged.
(6) sea-buckthorn perilla herb oil soft capsule is on the impact of Serum Lipids in Experimental HypercholesterolemicRats
By following table as seen, with 2.0, the sea-buckthorn perilla oil soft capsule product oil gavage of 3.0g/kgbw dosage group, in the time of the 28th day, compare with the water control group, serum TC decline percentage is respectively 12.1,7.2, compares with oily control group, and serum TC decline percentage is respectively 17.6,13.0.With 2.0, the sea-buckthorn perilla herb oil soft capsule product oil gavage of 3.0g/kgbw dosage group, in the time of the 28th day, compare with the water control group, serum TG decline percentage is respectively 29.3,26.1, compares with oily control group, serum TG decline percentage is respectively 31.6,28.4.
Table 1 sea-buckthorn perilla herb oil soft capsule is on the impact of Serum Lipids in Experimental HypercholesterolemicRats
5, conclusion
Experimental result shows: give middle dosage group (2.0g/kgbw) and high dose group (3.0g/kgbw) sea-buckthorn perilla herb oil soft capsule and can make and feed serum total cholesterol (TC) concentration raise the high lipid food rat and significantly be lower than water control group and oily control group; Serum triglyceride (TG) concentration significantly is lower than water control group and oily control group.High dose group Serum HDL-C/TC ratio also is significantly higher than water control group and oily control group simultaneously.According to the criterion in " function of health food is learned assessment process and the method for inspection ", think that sea-buckthorn perilla herb oil soft capsule has regulating blood lipid action.
Experimental example 2:
The inventor entrusts Gansu Prov. Medical Science Research Inst., according to " toxicological evaluation of food safety procedure and method ", sea-buckthorn perilla herb oil flexible glue has been carried out the toxicological assessment test.
1, animal used as test
Kunming mouse, body weight 18-22g, the Wistar rat, body weight 170-190g is the cleaning level.
2, experimental result
Acute toxicity test: select each 10 of Kunming mouse and rat male and female, carry out gavage contamination, general signs, poisoning symptom and the death condition of observing animal in 14 days by 18.4g/kgbw dosage.Calculate the LD50 value with the method for limiting the quantity of of maximum once, and carry out toxicity grading according to acute toxicity grading criteria.Result's each treated animal of rear Mouse and rat of contaminating has no poisoning symptom and death condition within the observation period, animal health is active, and Normal-weight increases.Female male animal LD50 all points out this tested material to belong to nontoxic level greater than 18.4g/kgbw.
Salmonella reversion test: adopt flat board to mix method, sample is established 5 dosage groups, establishes simultaneously from beaming back to become control group, solvent control group and positive controls, and parallel 3 wares of doing of each dosage group, test repeats once.Experimental result: each dosage group of twice test of sample is on average returned and is become the bacterium colony number average above certainly beaming back the twice that becomes clump count, also without dosage-reaction relation.The result is negative for the prompting Salmonella reversion test.
Bone marrow micronucleus test: select 50 of Kunming mouses, male and female half and half, be divided at random three various dose of sample (2.5,5.0,10g/kgbw), solvent control group (the ready-mixed oil 20g/kgbw that does not contain effective component), positive controls (endoxan 40mg/kgbw, lumbar injection).Each 5 of every group of male and female, 24h twice gavage in interval put to death animal in 6 hours after the gavage for the second time, got the micronucleus cell number that femur bone marrow checks polychromatic erythrocyte.Positive controls micronucleus cell number is apparently higher than solvent control group and tested material group (p＜0.01) as a result, and the micronucleus cell number of each dosage group of tested material and solvent control group are relatively without significant difference (p＞0.05), and the result is negative for the prompting bone marrow micronucleus test.
Sperm malformation test: select 25 of Male Kunming strain mice, be divided at random three various dose of sample (2.5,5.0,10g/kgbw), solvent control group (the ready-mixed oil 20g/kgbw that does not contain effective component), positive controls (endoxan 40mg/kgbw, lumbar injection).Every group 5, every day gavage once, continuous 5 days, put to death animal, and checked sperm morphology and quantity in the 35th day after giving first tested material.Positive controls teratospermia number is apparently higher than solvent control group and tested material group, meaning that there was a significant difference as a result.The teratospermia number of each dosage group of tested material and solvent control group comparing difference are without significant, and prompting mouse sperm deformity result of the test is negative.
The present invention examines through State Food and Drug Administration, thinks that sea-buckthorn perilla herb oil soft capsule product meets the regulation approval registration of " health food management method ", and authentication code is [the strong word G20040121 of state's food].
The above only is preferred embodiment of the present invention, and is in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the sea-buckthorn perilla herb oil soft capsule of an auxiliary lipid-lowering function, the percentage by weight that it is characterized in that its softgel shell is: edible gelatin 40%～45%, glycerine 18%～25%, pure water 35%～40%, the weight percent of its content is: Seabuckthorm Seed Oil 30～50%, Purple Perilla Seed Oil 70～50%.
2. the sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function as claimed in claim 1 is characterized in that described its softgel shell optimum weight percentage: edible gelatin 42%, glycerine 20%, pure water 38%.
3. the sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function as claimed in claim 1 is characterized in that described its content optimum weight percentage is: Seabuckthorm Seed Oil is 50%, Purple Perilla Seed Oil is 50%.
4. the sea-buckthorn perilla herb oil soft capsule of auxiliary lipid-lowering function as claimed in claim 2 is characterized in that containing in every 100g content effective component: oleic acid 21.1g ± 10%g, linoleic acid 23.0g ± 10%g, leukotrienes 44.3g ± 20%g, natural VE 〉=0.12g.
5. such as the preparation method of the sea-buckthorn perilla herb oil soft capsule of the described auxiliary lipid-lowering function of claims 1 to 3, it is characterized in that the preparation method of Seabuckthorm Seed Oil, the steps include:
Behind seabuckthorn seeds raw material drying, pulverizing 40～60 orders, take by weighing the seabuckthorn seeds raw material, drop in the supercritical extraction reactor, close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 20～22Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 15～20kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 2h under these conditions, obtains the seabuckthorn seeds crude oil; The seabuckthorn seeds crude oil that extraction obtains separates through supercentrifuge, and centrifuge speed is 16000～18000n/min, obtains the transparent Seabuckthorm Seed Oil of brownish red.
6. such as the preparation method of the sea-buckthorn perilla herb oil soft capsule of the described auxiliary lipid-lowering function of claims 1 to 3, it is characterized in that the preparation method of Purple Perilla Seed Oil, the steps include:
Behind purple perilla seed raw material drying, pulverizing 40～60 orders, take by weighing the purple perilla seed raw material, drop in the supercritical extraction reactor, close the extraction kettle cover, import supercritical CO by high-pressure pump
2Fluid, at extracting pressure 22～24Mpa, under 35～40 ℃ of conditions of extraction temperature, control CO
2Flow 80～100kg/h extracts; Extract enters separating still, is depressurized to 7.0～6.5Mpa, is warming up to 40～45 ℃ and resolves separation, and continuous extraction separates 6h under these conditions, obtains purple perilla seed crude oil; The purple perilla seed crude oil that extraction obtains is centrifugal through supercentrifuge, and centrifuge speed is 16000～18000n/min, obtains faint yellow transparent violet perilla oil.
7. such as the preparation method of the sea-buckthorn perilla herb oil soft capsule of the described auxiliary lipid-lowering function of claims 1 to 3, it is characterized in that the preparation method of sea-buckthorn perilla oil soft capsule, the steps include:
Add glycerine 18%～25% by claim 1 percentage by weight first in glue pot, pure water 35%～40% adds edible gelatin 40%～45% after stirring and be heated to 75～80 ℃, stirs 60～90min, and gelatin is dissolved fully; Open vavuum pump, continue to stir 45～60min under 0.06～0.08MPa vacuum condition, carry out vacuumize degassing, stop after degassed the finishing stirring, close vavuum pump, glue leaves standstill cooling 4h; With emulsification in 30～50% Seabuckthorm Seed Oils and the composite adding emulsion tank of 70～50% Purple Perilla Seed Oils, stir; Sea-buckthorn perilla herb oil after the emulsification is evenly carried out pelleting, 18～24 ℃ of pelleting temperature; The capsule and pill of compacting enters and rolls cage typing drying, and rolling the cage rotating speed is 40～50r/min, shaping time 〉=4h, to enter basin natural drying from rolling cage after the capsule and pill typing, and 22～27 ℃ of indoor temperatures, relative humidity are≤50%, time is 30～36h, dry good sea-buckthorn perilla herb oil soft capsule alcohol disinfecting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104569065A CN102907677A (en) | 2012-11-14 | 2012-11-14 | Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104569065A CN102907677A (en) | 2012-11-14 | 2012-11-14 | Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102907677A true CN102907677A (en) | 2013-02-06 |
Family
ID=47606439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104569065A Pending CN102907677A (en) | 2012-11-14 | 2012-11-14 | Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102907677A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238846A (en) * | 2013-05-31 | 2013-08-14 | 烟台燕园科玛生物技术开发有限公司 | Alpha-linolenic acid soft capsule |
| CN103584090A (en) * | 2013-10-22 | 2014-02-19 | 山西五台山沙棘制品有限公司 | Xiangzi soft capsule and preparation method thereof |
| CN103598587A (en) * | 2013-11-20 | 2014-02-26 | 山西振东制药股份有限公司 | Health-care food with blood fat reduction assistance effect and preparation method thereof |
| CN103655970A (en) * | 2013-11-18 | 2014-03-26 | 山西五台山沙棘制品有限公司 | Hawthorn and rhizoma alismatis seed soft capsule and preparation method thereof |
| CN104522666A (en) * | 2015-01-04 | 2015-04-22 | 吉林省农业科学院 | Dahurian rose fruit seed oil and purple perilla seed oil composite microcapsule capable of assisting in reducing blood fat and preparation method thereof |
| CN104688844A (en) * | 2015-03-17 | 2015-06-10 | 苏州市天灵中药饮片有限公司 | Traditional Chinese medicine soft capsule for protecting gastric mucosa and preparation method thereof |
| CN104946385A (en) * | 2015-06-24 | 2015-09-30 | 洛阳国花坊牡丹生物科技有限公司 | Supercritical extraction method of pumpkin seed oil |
| CN105038945A (en) * | 2015-06-24 | 2015-11-11 | 洛阳国花坊牡丹生物科技有限公司 | Supercritical extraction method of pomegranate seed oil |
| CN105285980A (en) * | 2015-10-14 | 2016-02-03 | 李红 | Compound health-care safflower oil spraying agent and preparation method thereof |
| CN106265785A (en) * | 2016-08-12 | 2017-01-04 | 张掖市润星生物科技有限公司 | A kind of Fructus Hippophae depression capsule and production technology thereof |
| CN106617078A (en) * | 2017-01-22 | 2017-05-10 | 四川创生生物科技有限公司 | Composition capable of lowering hyperglycemia, hyperlipemia and hypertension and compounded soft capsules prepared from composition |
| CN107836533A (en) * | 2016-09-19 | 2018-03-27 | 张黎平 | It is a kind of can external application beauty care edible purple perilla seed essential oil |
| CN108936671A (en) * | 2017-05-25 | 2018-12-07 | 无限极（中国）有限公司 | A kind of eliminating the phlegm stasis of blood composition and its preparation method and application |
| CN109007839A (en) * | 2018-08-13 | 2018-12-18 | 王鸿雁 | A kind of health-care edible capsule and preparation method thereof with auxiliary lipid-lowering efficacy |
| CN111407695A (en) * | 2020-03-27 | 2020-07-14 | 陕西海天制药有限公司 | Sea-buckthorn water and preparation method and application thereof |
| CN114081181A (en) * | 2021-11-15 | 2022-02-25 | 成都华西康健生物工程有限公司 | Food for treating hyperlipidemia, fatty liver and coronary heart disease and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145400A (en) * | 1995-09-11 | 1997-03-19 | 贵州省生物资源超临界流体萃取研究开发中心 | Method of extracting perilla plant oil by using super-critical CO2 |
| CN1370560A (en) * | 2001-02-27 | 2002-09-25 | 廖秀义 | Intelligence growth promoting antilipenic nutritous health care soft capsule and its prepn |
| US20060252949A1 (en) * | 2003-09-25 | 2006-11-09 | Lalit Chordia | Recovery of residual specialty oil |
| CN101011463A (en) * | 2005-06-08 | 2007-08-08 | 广东医学院 | Use of perilla oil in the preparation of medicament preparation for preventing and treating osteoporosis and skin senescence |
| CN101961378A (en) * | 2010-07-01 | 2011-02-02 | 高原圣果沙棘制品有限公司 | Formula of health-care product for resisting oxidation and enhancing immunity |
-
2012
- 2012-11-14 CN CN2012104569065A patent/CN102907677A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145400A (en) * | 1995-09-11 | 1997-03-19 | 贵州省生物资源超临界流体萃取研究开发中心 | Method of extracting perilla plant oil by using super-critical CO2 |
| CN1370560A (en) * | 2001-02-27 | 2002-09-25 | 廖秀义 | Intelligence growth promoting antilipenic nutritous health care soft capsule and its prepn |
| US20060252949A1 (en) * | 2003-09-25 | 2006-11-09 | Lalit Chordia | Recovery of residual specialty oil |
| CN101011463A (en) * | 2005-06-08 | 2007-08-08 | 广东医学院 | Use of perilla oil in the preparation of medicament preparation for preventing and treating osteoporosis and skin senescence |
| CN101961378A (en) * | 2010-07-01 | 2011-02-02 | 高原圣果沙棘制品有限公司 | Formula of health-care product for resisting oxidation and enhancing immunity |
Non-Patent Citations (2)
| Title |
|---|
| 姜明珠: "沙棘籽油的提取分离技术研究", 《吉林农业大学》 * |
| 武汉时代欣华生物科技有限公司: "沙棘苏子油软胶囊(芯脉舒)", 《HTTP://EXPO.PHARMNET.COM.CN/PRO_PRODUCT/LIST--PRODUCT--30650--1.HTML》 * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238846B (en) * | 2013-05-31 | 2015-03-18 | 烟台燕园科玛生物技术开发有限公司 | Alpha-linolenic acid soft capsule |
| CN103238846A (en) * | 2013-05-31 | 2013-08-14 | 烟台燕园科玛生物技术开发有限公司 | Alpha-linolenic acid soft capsule |
| CN103584090B (en) * | 2013-10-22 | 2015-10-28 | 山西五台山沙棘制品有限公司 | Blood fat reducing soft capsule and preparation method thereof |
| CN103584090A (en) * | 2013-10-22 | 2014-02-19 | 山西五台山沙棘制品有限公司 | Xiangzi soft capsule and preparation method thereof |
| CN103655970A (en) * | 2013-11-18 | 2014-03-26 | 山西五台山沙棘制品有限公司 | Hawthorn and rhizoma alismatis seed soft capsule and preparation method thereof |
| CN103598587A (en) * | 2013-11-20 | 2014-02-26 | 山西振东制药股份有限公司 | Health-care food with blood fat reduction assistance effect and preparation method thereof |
| CN104522666A (en) * | 2015-01-04 | 2015-04-22 | 吉林省农业科学院 | Dahurian rose fruit seed oil and purple perilla seed oil composite microcapsule capable of assisting in reducing blood fat and preparation method thereof |
| CN104688844A (en) * | 2015-03-17 | 2015-06-10 | 苏州市天灵中药饮片有限公司 | Traditional Chinese medicine soft capsule for protecting gastric mucosa and preparation method thereof |
| CN104946385A (en) * | 2015-06-24 | 2015-09-30 | 洛阳国花坊牡丹生物科技有限公司 | Supercritical extraction method of pumpkin seed oil |
| CN105038945A (en) * | 2015-06-24 | 2015-11-11 | 洛阳国花坊牡丹生物科技有限公司 | Supercritical extraction method of pomegranate seed oil |
| CN105285980A (en) * | 2015-10-14 | 2016-02-03 | 李红 | Compound health-care safflower oil spraying agent and preparation method thereof |
| CN106265785A (en) * | 2016-08-12 | 2017-01-04 | 张掖市润星生物科技有限公司 | A kind of Fructus Hippophae depression capsule and production technology thereof |
| CN107836533A (en) * | 2016-09-19 | 2018-03-27 | 张黎平 | It is a kind of can external application beauty care edible purple perilla seed essential oil |
| CN106617078A (en) * | 2017-01-22 | 2017-05-10 | 四川创生生物科技有限公司 | Composition capable of lowering hyperglycemia, hyperlipemia and hypertension and compounded soft capsules prepared from composition |
| CN108936671A (en) * | 2017-05-25 | 2018-12-07 | 无限极（中国）有限公司 | A kind of eliminating the phlegm stasis of blood composition and its preparation method and application |
| CN109007839A (en) * | 2018-08-13 | 2018-12-18 | 王鸿雁 | A kind of health-care edible capsule and preparation method thereof with auxiliary lipid-lowering efficacy |
| CN111407695A (en) * | 2020-03-27 | 2020-07-14 | 陕西海天制药有限公司 | Sea-buckthorn water and preparation method and application thereof |
| CN111407695B (en) * | 2020-03-27 | 2022-09-30 | 陕西海天制药有限公司 | Sea-buckthorn water and preparation method and application thereof |
| CN114081181A (en) * | 2021-11-15 | 2022-02-25 | 成都华西康健生物工程有限公司 | Food for treating hyperlipidemia, fatty liver and coronary heart disease and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102907677A (en) | Sea-buckthorn perilla seed oil soft capsule having function of assisting blood-fat reduction and preparation method thereof | |
| CN103385315A (en) | Vegetable oil composition with hypolipidemic effect as well as preparation method and application thereof | |
| CN103145776A (en) | Gastrodin extraction method, preparation method of gastrodia elata soft capsules and soft capsules obtained by preparation method | |
| CN106214773B (en) | A kind of pair of chemical damage has composition of assistant protection function and preparation method thereof | |
| CN102551065A (en) | Blood sugar reducing food series | |
| CN105394749B (en) | A kind of Ricipe for health care food and preparation method and application containing astaxanthin | |
| CN106722934A (en) | A kind of anti-ageing health food for containing peptide extract containing earthworm, clam worm | |
| CN106138101A (en) | A kind of preparation method of Embryo Gallus domesticus extract | |
| US10227547B2 (en) | Astragalus membranaceus seed raw oil, refined oil, preparation method therefor and use thereof | |
| CN104255988A (en) | Tea type capsules and production method thereof | |
| CN108077929A (en) | A kind of compound fructus cannabis oil oxidation resisting soft capsule and its production method | |
| CN106562165A (en) | Slimming type blended fruit-vegetable juice drink and preparation method thereof | |
| CN101234160A (en) | Fat-reducing and liver-protecting Chinese medicine preparation and preparation thereof | |
| CN103229872A (en) | Anti-aging food health-care tea electuary | |
| CN100402052C (en) | Blood fat-reducing medicine and its preparation method | |
| KR20010091416A (en) | A marking method of health-help food, medical composite and special-nutritious food for improve the corpulence and the food | |
| CN109652202A (en) | A kind of intelligence development safflower tea oil and preparation method thereof | |
| CN105661542A (en) | Lipid-reducing healthcare oil | |
| CN102106534A (en) | Composition for assisting in lowering blood fat and preparation method thereof | |
| CN104783159A (en) | Nutrient enhancer for middle-aged goat milk powder and preparation method thereof | |
| CN101229233B (en) | Chinese traditional medicine preparation for antiatheroscloresis and liver-protection, and preparingng method thereof | |
| CN114010676A (en) | Composition for reducing blood fat and improving memory and preparation method thereof | |
| CN105360787A (en) | Health food and preparation method thereof | |
| CN102580108A (en) | Novel oily liquid auxiliary material for soft capsules for reducing blood fat | |
| CN101342241B (en) | White Chinese olive angelica oil soft capsule and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130206 |