CN103484391A - 一种胞外α-酮戊二酸产量提高的解脂亚洛酵母基因工程菌 - Google Patents
一种胞外α-酮戊二酸产量提高的解脂亚洛酵母基因工程菌 Download PDFInfo
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- CN103484391A CN103484391A CN201310481832.5A CN201310481832A CN103484391A CN 103484391 A CN103484391 A CN 103484391A CN 201310481832 A CN201310481832 A CN 201310481832A CN 103484391 A CN103484391 A CN 103484391A
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- lipolytica
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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Abstract
Description
Claims (6)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310481832.5A CN103484391B (zh) | 2013-10-15 | 2013-10-15 | 一种胞外α-酮戊二酸产量提高的解脂亚洛酵母基因工程菌 |
US14/142,897 US9328363B2 (en) | 2013-10-15 | 2013-12-29 | Genetically engineered Yarrowia lipolytica with enhanced extracellular secretion of α-ketoglutarate |
Applications Claiming Priority (1)
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CN201310481832.5A CN103484391B (zh) | 2013-10-15 | 2013-10-15 | 一种胞外α-酮戊二酸产量提高的解脂亚洛酵母基因工程菌 |
Publications (2)
Publication Number | Publication Date |
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CN103484391A true CN103484391A (zh) | 2014-01-01 |
CN103484391B CN103484391B (zh) | 2015-03-25 |
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CN201310481832.5A Active CN103484391B (zh) | 2013-10-15 | 2013-10-15 | 一种胞外α-酮戊二酸产量提高的解脂亚洛酵母基因工程菌 |
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US (1) | US9328363B2 (zh) |
CN (1) | CN103484391B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104357344A (zh) * | 2014-11-19 | 2015-02-18 | 江南大学 | 一种强化解脂亚洛酵母合成α-酮戊二酸的方法 |
CN105177065A (zh) * | 2015-09-11 | 2015-12-23 | 浙江树人大学 | 一种生物转化法合成α-酮戊二酸的方法 |
CN109536396A (zh) * | 2017-09-22 | 2019-03-29 | 现代牧场股份有限公司 | 重组酵母菌株 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101215529A (zh) * | 2007-12-26 | 2008-07-09 | 江南大学 | 一种α-酮戊二酸高产菌及其筛选方法和用该菌株发酵法生产α-酮戊二酸 |
CN102071154A (zh) * | 2010-12-08 | 2011-05-25 | 江南大学 | 一种产α-酮戊二酸酵母工程菌及其构建方法 |
CN102586128A (zh) * | 2012-03-14 | 2012-07-18 | 江南大学 | 一种高产α-酮戊二酸酵母工程菌及其应用 |
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2013
- 2013-10-15 CN CN201310481832.5A patent/CN103484391B/zh active Active
- 2013-12-29 US US14/142,897 patent/US9328363B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101215529A (zh) * | 2007-12-26 | 2008-07-09 | 江南大学 | 一种α-酮戊二酸高产菌及其筛选方法和用该菌株发酵法生产α-酮戊二酸 |
CN102071154A (zh) * | 2010-12-08 | 2011-05-25 | 江南大学 | 一种产α-酮戊二酸酵母工程菌及其构建方法 |
CN102586128A (zh) * | 2012-03-14 | 2012-07-18 | 江南大学 | 一种高产α-酮戊二酸酵母工程菌及其应用 |
Non-Patent Citations (7)
Title |
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AGNÈS HÉBERT ET AL.: "New Insights into Sulfur Metabolism in Yeasts as Revealed by Studies of Yarrowia lipolytica", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》, vol. 79, no. 4, 28 February 2013 (2013-02-28), pages 1200 - 1211 * |
DUJON,B. ET AL.: "XM_501098、XM_502090、XM_503058、XM_504706", 《GENBANK》, 29 October 2008 (2008-10-29) * |
JINGWEN ZHOU ET AL.: "Enhanced α-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism", 《JOURNAL OF BIOTECHNOLOGY》, vol. 161, 31 December 2012 (2012-12-31), pages 257 - 264 * |
SUSAN M. HUTSON ET AL.: "Identification of the Mitochondrial Branched Chain Aminotransferase as a Branched Chain a-KetoA cid Transport Protein", 《THE JOURNALOF BIOLOGICACL HEMISTR》, vol. 268, no. 5, 31 December 1993 (1993-12-31), pages 3084 - 3091 * |
XIAOXIA YIN ET AL.: "Enhanced alpha-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06 by regulation of the pyruvate carboxylation pathway", 《APPL MICROBIOL BIOTECHNOL》, vol. 96, 31 December 2012 (2012-12-31), pages 1527 - 1537 * |
池振明 等: "解脂耶罗维亚酵母(Yarrowia lipolytica)的代谢工程", 《中国海洋大学学报》, vol. 42, no. 5, 31 May 2012 (2012-05-31), pages 55 - 59 * |
郭洪伟 等: "微生物发酵生产α-酮戊二酸研究进展", 《生物工程学报》, vol. 29, no. 2, 25 February 2013 (2013-02-25), pages 141 - 152 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104357344A (zh) * | 2014-11-19 | 2015-02-18 | 江南大学 | 一种强化解脂亚洛酵母合成α-酮戊二酸的方法 |
CN105177065A (zh) * | 2015-09-11 | 2015-12-23 | 浙江树人大学 | 一种生物转化法合成α-酮戊二酸的方法 |
CN109536396A (zh) * | 2017-09-22 | 2019-03-29 | 现代牧场股份有限公司 | 重组酵母菌株 |
Also Published As
Publication number | Publication date |
---|---|
CN103484391B (zh) | 2015-03-25 |
US20150104794A1 (en) | 2015-04-16 |
US9328363B2 (en) | 2016-05-03 |
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Address after: 214122 Jiangsu city of Wuxi Province District Liangxi No. 898 South Road 7 layer beam Creek area of food science and Technology Park Patentee after: Jiangnan University Address before: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Patentee before: Jiangnan University |
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