CN103462867A - Preparation method of silk glue/trehalose skin-care facial mask - Google Patents
Preparation method of silk glue/trehalose skin-care facial mask Download PDFInfo
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- CN103462867A CN103462867A CN2013104005442A CN201310400544A CN103462867A CN 103462867 A CN103462867 A CN 103462867A CN 2013104005442 A CN2013104005442 A CN 2013104005442A CN 201310400544 A CN201310400544 A CN 201310400544A CN 103462867 A CN103462867 A CN 103462867A
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- sericin
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Abstract
The invention relates to the technical field of skin-care products and discloses a preparation method of a silk glue/trehalose skin-care facial mask. The preparation method comprises the steps of shearing silkworm cocoon shells into pieces with crustacean sizes, soaking the cocoon shell pieces in an aqueous solution, washing, soaking in deionized water for swelling for 20min, carrying out high-temperature (120 DEG C) high-pressure treatment for 30min, and centrifuging to remove impurities to obtain a sericin aqueous solution with the low molecular weight; then processing by using a freeze drying technique to form the silk glue/trehalose skin-care facial mask. The facial mask prepared by using the method has no stimulation to the skin and no allergic reaction, and has good affinity with the skin. The sericin with the low molecular weight, extracted by using the method, can be effectively absorbed by the skin, an extraction method is simple, and reagents used for acid degradation, alkaline hydrolysis, organic solvent dissolution, and enzymolysis and harmful to the skin in the traditional method are avoided.
Description
Technical field
The present invention relates to technical field of skin care, specifically, is the preparation method of a kind of sericin/trehalose facial treatment mask.
Background technology
Sericin is a kind of water solublity globular preteins that is coated on natural silk fiber periphery, because it has the effect of Promote cell's growth and propagation, in technical field of biological material (as skin wound dressing materials etc.) extensive application.Sericin is comprised of 18 seed amino acids.Tyrosine in sericin (Tyr), tryptophan (Trp), phenylalanine (Phe) etc. can effectively absorb ultraviolet, and sericin can effectively be prevented to the damage of ultraviolet to skin for skin care item.What the sericin larger from molecular weight was different is, low-molecular-weight sericin is soluble in water, can be absorbed by the skin fully, promote the functions such as subcutaneous blood circulation, improve the crease-resistant and wettability of skin, give skin crease-resistant, moist, whitening and smooth, keep muchly skin wetness, make skin health natural, flexible.Have report research can improve the stability of product with sericin as the skin care item of raw material, after the very short time insulation, formation one deck can perceptible even protecting film, obviously reduces the epidermis dehydration.Sericin also has application for a long time as the raw material of the skincare products such as bath gel and hair conditioner.These characteristics make sericin become the natural cosmetics raw material of a kind of preciousness in cosmetics industry.
Medically successfully using the trehalose instead plasma protein as blood products, the stabilizing agent of the bioactive substance such as vaccine, lymphocyte, cell tissue.Simultaneously, natural trehalose is a kind of multi-functional effective skin protection raw material, can play wrinkle removing to skin, removes speckle, whitening, and anti-inflammatory anti-itch increases immunity and the protective effect of skin.Black speck has repair, and calm tired, rough skin makes skin remain fine and smooth, glossy.Due to the stability of character, trehalose not only can normal temperature condition under dry long-term storage, also can deposit and never degenerate for a long time as the wet type facial film.
Summary of the invention
The present invention be take natural material silk proteine hydrolysate (sericin) and trehalose and is prepared facial film by certain processing method as raw material, and concrete technical scheme of the present invention is as follows:
The present invention is the preparation method of a kind of sericin/trehalose facial treatment mask, and concrete preparation process is as follows:
(1) the tasar silkworm cocoon shell is cut into to fingernail size lamellar, lamellar cocoon shell be impregnated in aqueous solution, be soaked in swelling 20min in deionized water after washes clean, through high temperature (120 ℃) HIGH PRESSURE TREATMENT 30min, centrifugal remove out impurity after, resulting aqueous solution is low-molecular-weight sericin aqueous solution;
(2) the sericin aqueous solution is removed to the impurity in solution through high speed centrifugation, then solution is concentrated to 2-20wt% with Rotary Evaporators, sericin powder is made in last lyophilization;
(3) a certain amount of sericin powder, trehalose are mixed with a certain amount of deionized water, water-bath 60-90 ℃ dissolving, evenly obtain gelatinous solution by magnetic stirrer in course of reaction;
(4) gelatinous solution is fallen on the mould polyethylene board, at 25 ℃, make its gel solidification in the environment of humidity 50%, obtain dressing membrane;
(5) utilize Freeze Drying Technique machine-shaping to obtain sericin/trehalose facial film.
As further improvement, step of the present invention (5) is dressing membrane now to be ground to existing use make the smear type facial film, in alternative steps (5), utilizes Freeze Drying Technique machine-shaping to obtain sericin/trehalose facial film.
As further improvement, sericin of the present invention/trehalose facial film can add one or more in functional additive, the antioxidant activity factor, freshener, aromatic antiseptic.
Beneficial effect of the present invention is as follows:
The facial film of producing by this method is non-stimulated to skin, and without anaphylaxis, the affinity of facial film and skin is good.The low-molecular-weight sericin extracted by this method can effectively be absorbed by the skin, and extracting method is simple, the reagent that the acid of having avoided using in the traditional method is fallen, alkaline hydrolysis, organic solvent dissolution, enzymolysis etc. are harmful to skin.The present invention prepares sericin used and trehalose contains various bioactivators and the skin-nourishing factor separately, realizes being absorbed by the skin, without adding extra active component with having complementary advantages again.But long-term storage under the facial film room temperature aseptic condition of making.The invention provides a kind of processing technique comparatively simply and easily.
The specific embodiment
Below by specific embodiment, technical scheme of the present invention is described further:
Embodiment 1
(1) the tasar silkworm cocoon shell is cut into to fingernail size lamellar, lamellar cocoon shell be impregnated in aqueous solution, be soaked in swelling 20min in deionized water after washes clean, high temperature (120 ℃) high pressure 30min, resulting aqueous solution is the sericin aqueous solution;
(2) the sericin aqueous solution is removed to the impurity in solution through high speed centrifugation, be concentrated to 10wt%, sericin powder is made in last lyophilization;
(3) a certain amount of sericin powder, trehalose are mixed with a certain amount of deionized water, 80 ℃ of dissolving 1h of water-bath, even by magnetic stirrer in course of reaction;
(4) pour the gelatinous solution obtained in (3) into the molding vessel, make at normal temperatures its partial gel solidify, obtain dressing membrane;
(5) by the dressing membrane obtained, put into-60 ℃ of dry 24h of freezer dryer, obtain sericin/trehalose facial film.
Embodiment 2
(1) the tasar silkworm cocoon shell is cut into to fingernail size lamellar, lamellar cocoon shell be impregnated in aqueous solution, be soaked in swelling 20min in deionized water after washes clean, high temperature (120 ℃) high pressure 60min, resulting aqueous solution is the sericin aqueous solution;
(2) the sericin aqueous solution is removed to the impurity in solution through high speed centrifugation, reconcentration is to 8wt%, and sericin powder is made in last lyophilization;
(3) a certain amount of sericin powder, trehalose are mixed with a certain amount of deionized water, 60 ℃ of dissolving 2h of water-bath, evenly obtain gelatinous solution by magnetic stirrer in course of reaction, can make the smear type facial film.
Embodiment 3
(1) the tasar silkworm cocoon shell is cut into to fingernail size lamellar, lamellar cocoon shell be impregnated in aqueous solution, be soaked in swelling 20min in deionized water after washes clean, at autoclave the inside high temperature (120 ℃) high pressure 60min, resulting aqueous solution is low-molecular-weight sericin aqueous solution afterwards;
(2) the sericin aqueous solution is removed to the impurity in solution through high speed centrifugation, then be concentrated to 10wt% through Rotary Evaporators, sericin powder is made in last lyophilization;
(3) a certain amount of sericin powder, trehalose are mixed with a certain amount of deionized water, 60 ℃ of dissolving 2h of water-bath, evenly obtain gelatinous solution by magnetic stirrer in course of reaction;
(4) pour the gelatinous solution obtained in (3) into the molding vessel, make at normal temperatures its partial gel solidify, obtain dressing membrane;
(5) utilize Freeze Drying Technique machine-shaping to obtain sericin/trehalose facial film.
Finally, it is also to be noted that, what more than enumerate is only specific embodiments of the invention.Obviously, the invention is not restricted to above examples of implementation, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (3)
1. the preparation method of sericin/trehalose facial treatment mask, is characterized in that, concrete preparation process is as follows:
(1) the tasar silkworm cocoon shell is cut into to fingernail size lamellar, lamellar cocoon shell be impregnated in aqueous solution, be soaked in 20min in deionized water after washes clean, through high temperature (120 ℃) HIGH PRESSURE TREATMENT 30min, resulting aqueous solution is low-molecular-weight sericin aqueous solution;
(2) the sericin aqueous solution is removed to the impurity in solution through high speed centrifugation, then with Rotary Evaporators, solution is concentrated to 2-20wt%, sericin powder is made in last lyophilization;
(3) a certain amount of sericin powder, trehalose are mixed with a certain amount of deionized water, water-bath 60-90 ℃ dissolving, evenly obtain gelatinous solution by magnetic stirrer in course of reaction;
(4) gelatinous solution is fallen on the mould polyethylene board, at 25 ℃, make its gel solidification in the environment of humidity 50%, obtain dressing membrane;
(5) utilize Freeze Drying Technique machine-shaping to obtain sericin/trehalose facial film.
2. the preparation method of sericin according to claim 1/trehalose facial treatment mask, it is characterized in that, described step (5) is dressing membrane now to be ground to existing use make the smear type facial film, in alternative steps (5), utilizes Freeze Drying Technique machine-shaping to obtain sericin/trehalose facial film.
3. the preparation method of sericin according to claim 1 and 2/trehalose facial treatment mask, is characterized in that, described sericin/trehalose facial film can add one or more in functional additive, the antioxidant activity factor, freshener, aromatic antiseptic.
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| CN2013104005442A CN103462867A (en) | 2013-09-05 | 2013-09-05 | Preparation method of silk glue/trehalose skin-care facial mask |
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| CN2013104005442A CN103462867A (en) | 2013-09-05 | 2013-09-05 | Preparation method of silk glue/trehalose skin-care facial mask |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490711A (en) * | 2014-12-30 | 2015-04-08 | 广州丹奇日用化工厂有限公司 | Edible smoothing toner and preparation method thereof |
| CN104523451A (en) * | 2014-12-30 | 2015-04-22 | 广州丹奇日用化工厂有限公司 | Edible toning lotion and preparing method of edible toning lotion |
| CN106074209A (en) * | 2016-07-06 | 2016-11-09 | 中绿农生物科技有限公司 | Tea tree ethereal oil Nano capsule slow release is without paper film |
| CN106726681A (en) * | 2016-12-15 | 2017-05-31 | 浙江大学 | A kind of method that sericin prepares mask sheet |
| CN106726631A (en) * | 2016-12-17 | 2017-05-31 | 周世容 | A kind of smooth skin rejuvenating facial mask and preparation method thereof |
| CN107260576A (en) * | 2017-05-16 | 2017-10-20 | 浙江金石生物科技有限公司 | A kind of facial mask congealed fat containing Dendrobium officinale polysaccharide body and preparation method thereof |
| CN108578761A (en) * | 2018-04-12 | 2018-09-28 | 西南大学 | Load the preparation method and applications of silk gum/Ago-Gel of lysozyme |
| CN109806199A (en) * | 2019-03-19 | 2019-05-28 | 成都大学 | A kind of whitening and skin-protecting composition and preparation method thereof |
| CN113633568A (en) * | 2021-09-08 | 2021-11-12 | 伊深细胞生物技术(上海)有限公司 | Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490711A (en) * | 2014-12-30 | 2015-04-08 | 广州丹奇日用化工厂有限公司 | Edible smoothing toner and preparation method thereof |
| CN104523451A (en) * | 2014-12-30 | 2015-04-22 | 广州丹奇日用化工厂有限公司 | Edible toning lotion and preparing method of edible toning lotion |
| CN106074209A (en) * | 2016-07-06 | 2016-11-09 | 中绿农生物科技有限公司 | Tea tree ethereal oil Nano capsule slow release is without paper film |
| CN106726681A (en) * | 2016-12-15 | 2017-05-31 | 浙江大学 | A kind of method that sericin prepares mask sheet |
| CN106726681B (en) * | 2016-12-15 | 2019-11-12 | 浙江大学 | A method of mask sheet is prepared with sericin |
| CN106726631A (en) * | 2016-12-17 | 2017-05-31 | 周世容 | A kind of smooth skin rejuvenating facial mask and preparation method thereof |
| CN107260576A (en) * | 2017-05-16 | 2017-10-20 | 浙江金石生物科技有限公司 | A kind of facial mask congealed fat containing Dendrobium officinale polysaccharide body and preparation method thereof |
| CN107260576B (en) * | 2017-05-16 | 2021-05-07 | 杭州千岛湖赋溪实业有限公司 | Facial mask gel containing dendrobium officinale polysaccharide and preparation method thereof |
| CN108578761A (en) * | 2018-04-12 | 2018-09-28 | 西南大学 | Load the preparation method and applications of silk gum/Ago-Gel of lysozyme |
| CN109806199A (en) * | 2019-03-19 | 2019-05-28 | 成都大学 | A kind of whitening and skin-protecting composition and preparation method thereof |
| CN109806199B (en) * | 2019-03-19 | 2021-09-03 | 成都大学 | Whitening skin care composition and preparation method thereof |
| CN113633568A (en) * | 2021-09-08 | 2021-11-12 | 伊深细胞生物技术(上海)有限公司 | Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin |
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Application publication date: 20131225 |