CN113633568A - Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin - Google Patents
Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin Download PDFInfo
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- CN113633568A CN113633568A CN202111048653.3A CN202111048653A CN113633568A CN 113633568 A CN113633568 A CN 113633568A CN 202111048653 A CN202111048653 A CN 202111048653A CN 113633568 A CN113633568 A CN 113633568A
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- 108010013296 Sericins Proteins 0.000 title claims abstract description 25
- 239000011550 stock solution Substances 0.000 title claims abstract description 11
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 10
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 10
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 238000001976 enzyme digestion Methods 0.000 title abstract description 5
- 239000000243 solution Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 13
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 13
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 239000012153 distilled water Substances 0.000 claims abstract description 12
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 12
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 11
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 11
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 230000006641 stabilisation Effects 0.000 claims abstract description 4
- 238000011105 stabilization Methods 0.000 claims abstract description 4
- 150000003254 radicals Chemical class 0.000 abstract description 12
- 235000018102 proteins Nutrition 0.000 abstract description 8
- 235000004252 protein component Nutrition 0.000 abstract description 5
- 239000003381 stabilizer Substances 0.000 description 7
- 230000007062 hydrolysis Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 230000000087 stabilizing effect Effects 0.000 description 4
- 108090000604 Hydrolases Proteins 0.000 description 3
- 102000004157 Hydrolases Human genes 0.000 description 3
- 208000028990 Skin injury Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000032683 aging Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 108010007119 flavourzyme Proteins 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 150000002840 non-reducing disaccharides Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000003223 protective agent Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- FCSHMCFRCYZTRQ-UHFFFAOYSA-N N,N'-diphenylthiourea Chemical compound C=1C=CC=CC=1NC(=S)NC1=CC=CC=C1 FCSHMCFRCYZTRQ-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 206010063493 Premature ageing Diseases 0.000 description 1
- 208000032038 Premature aging Diseases 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 206010040954 Skin wrinkling Diseases 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- -1 superoxide radicals Chemical class 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a preparation method of an antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin, which comprises the following steps: firstly, 100g of sericin powder is dissolved in 1L of distilled water to prepare solution A with the concentration of 0.1 g/ml; dissolving 100g protease in 1L distilled water to obtain solution B with pH of 7.0 and concentration of 0.1 g/ml; 10g of trehalose powder, 10g of hyaluronic acid according to 1: dissolving 1L of distilled water in the proportion of 1 to prepare a C solution with the concentration of 0.01 g/ml; heating the solution A to 50 ℃, adding the solution B (the ratio of enzyme to protein is 1: 100) according to the proportion of 1/100 of the solution A to maintain the temperature, and testing the pH value to be 7, wherein the pH value is maintained at 7 by using NAHO if the conditions are changed; after reacting for two hours, heating the solution to 80-100 ℃ to destroy the protease, stopping the hydrolysis reaction of the enzyme, cooling to room temperature, and adding 1/10C solution for stabilization. The present invention can maintain the stability of incompletely hydrolyzed protein component, and thus can reach the stable state of eliminating free radical optimally.
Description
Technical Field
The invention relates to the technical field of hydrolyzed sericin, in particular to a preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin.
Background
Free radicals initiate oxidation, so that the oxidation occurs in vitro, active oxidase is easily generated by light and air, the activation of the activity of the tyrosine is promoted, and the possibility of skin wrinkling, skin spots, blackening, aging and skin canceration is caused. Therefore, the skin surface free radical can be removed, the early-stage aging reaction can be effectively prevented, and the effect of resisting the premature aging can be achieved.
Sericin does not react with and remove free radicals because of a macromolecular stable structure, but a sericin incompletely hydrolyzed substance has strong capacity of removing hydroxyl and superoxide radicals; thus, it is intended to provide a hydrolyzed sericin which can stabilize an incompletely hydrolyzed protein component and can improve the radical scavenging ability.
Based on the method, the invention designs a preparation method of the antioxidant and anti-aging stock solution of the incomplete enzyme digestion sericin.
Disclosure of Invention
The invention aims to provide a preparation method of an antioxidant and anti-aging stock solution of incomplete enzyme-digested sericin, which aims to solve the problems that sericin proposed in the background art does not react with free radicals and remove the free radicals because of a macromolecular stable structure, but a sericin incomplete hydrolysis substance has strong capacity of removing hydroxyl and superoxide radical; thus, there has been proposed a problem that hydrolyzed sericin, which is capable of stabilizing an incompletely hydrolyzed protein component, can improve its ability to scavenge free radicals.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of an antioxidant and anti-aging stock solution of incomplete enzyme-digested sericin comprises the following steps:
the first step is as follows: dissolving 100g of sericin powder in 1L of distilled water to prepare solution A with the concentration of 0.1 g/ml;
the second step is that: dissolving 100g protease in 1L distilled water to obtain solution B with pH of 7.0 and concentration of 0.1 g/ml;
the third step: 10g of trehalose powder, 10g of hyaluronic acid according to 1: dissolving 1L of distilled water in the proportion of 1 to prepare a C solution with the concentration of 0.01 g/ml;
the fourth step: heating the solution A to 50 ℃, adding the solution B (the ratio of enzyme to protein is 1: 100) according to the proportion of 1/100 of the solution A to maintain the temperature, and testing the pH value to be 7, wherein the pH value is maintained at 7 by using NAHO if the conditions are changed;
the fifth step: after reacting for two hours, heating the solution to 80-100 ℃ to destroy the protease, stopping the hydrolysis reaction of the enzyme, cooling to room temperature, and adding 1/10C solution for stabilization.
Compared with the prior art, the invention has the beneficial effects that: trehalose and hyaluronic acid can protect hydrolyzed protein peptide from hydrolysis, trehalose is stable non-reducing disaccharide, has stable property, can form a special protective film on the surface of cells under the severe conditions of high temperature, high cold, drying and water loss, effectively protects the biomolecular structure from being damaged, is a very effective stabilizer and protective agent of bioactive substances, can keep the incompletely hydrolyzed protein components stable, and can achieve the stable state of optimally clearing free radicals, and the stabilizer with the ratio of trehalose to hyaluronic acid of 1:1 has the efficacy of stabilizing and repairing skin injury.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a graph showing the concentration ratio of enzymes and proteins in a plurality of groups and the clearance results of reaction time in a plurality of groups;
FIG. 2 is a graph comparing the results of the sum of squares of deviation, degrees of freedom, F-number and significance of solutions A and B;
FIG. 3 is a comparison of multiple sets of assay data for the composition of the incomplete hydrolysis of sericin amino acids by flavourzyme.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a preparation method of an antioxidant and anti-aging stock solution of incomplete enzyme-digested sericin comprises the following steps:
the first step is as follows: dissolving 100g of sericin powder in 1L of distilled water to prepare solution A with the concentration of 0.1 g/ml;
the second step is that: dissolving 100g protease in 1L distilled water to obtain solution B with pH of 7.0 and concentration of 0.1 g/ml;
the third step: 10g of trehalose powder, 10g of hyaluronic acid according to 1: dissolving 1L of distilled water in the proportion of 1 to prepare a C solution with the concentration of 0.01 g/ml;
the fourth step: heating the solution A to 50 ℃, adding the solution B (the ratio of enzyme to protein is 1: 100) according to the proportion of 1/100 of the solution A to maintain the temperature, and testing the pH value to be 7, wherein the pH value is maintained at 7 by using NAHO if the conditions are changed;
the fifth step: after reacting for two hours, heating the solution to 80-100 ℃ to destroy the protease, stopping the hydrolysis reaction of the enzyme, cooling to room temperature, and adding 1/10C solution for stabilization.
The second embodiment:
the results of comparing the clearance rates of the present embodiment by setting concentration ratios of multiple groups of enzymes and proteins and multiple groups of reaction times are shown in fig. 1-2, wherein the clearance rate is calculated by measuring the ability to scavenge free radicals (OH).
As can be seen from FIG. 1, the optimum conditions for the protease hydrolysis were a solution having a concentration of 0.1g/ml, a reaction temperature of 50 degrees Celsius, a hydrolysis time of 2 hours, a sericin concentration and an enzyme concentration of 100: the antioxidant capacity (namely the capacity of eliminating free radicals (OH)) is strongest when the compound is 1.
The third embodiment is as follows:
the present example examined the composition of the incompletely hydrolyzed sericin amino acid by flavourzyme, and compared the data after addition of the stabilizer C solution, and the results are shown in fig. 3.
As can be seen from FIG. 3, even if the hydrolysis reaction is stopped by the high-temperature destruction of the hydrolase, a part of the hydrolase continues to undergo the hydrolysis reaction with the sericin, and after the solution is left to stand for 1 hour, the mass spectrum shows that the substance of the hydrolase is changed, and the stable state for optimally scavenging the free radicals cannot be achieved. Therefore, after the stock solution is heated to destroy the enzyme, 1 percent of trehalose and hyaluronic acid stock solution (1: 1) are added to protect the hydrolyzed protein peptide from hydrolysis, the trehalose is stable non-reducing disaccharide, has very stable property, can form a special protective film on the surface of cells under the severe conditions of high temperature, high cold, drying and water loss, effectively protects the biomolecular structure from being destroyed, and is a very effective stabilizer and protective agent for bioactive substances. The stabilizer prepared from trehalose and hyaluronic acid at a ratio of 1:1 has the effects of stabilizing and repairing skin injury, especially sunburn. After addition of the stabilizer of trehalose and hyaluronic acid, the incompletely hydrolyzed protein fraction was again found to remain stable by mass spectrometry.
The hydrolyzed sericin of the invention is added with trehalose and hyaluronic acid as stabilizers, which can maintain the stability of incompletely hydrolyzed protein components, thereby achieving the stable state of optimally clearing free radicals, and the stabilizer with the ratio of trehalose to hyaluronic acid of 1:1 has the efficacy of stabilizing and repairing skin injury.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (1)
1. A preparation method of an antioxidant and anti-aging stock solution of incomplete enzyme-digested sericin is characterized by comprising the following steps:
the first step is as follows: dissolving 100g of sericin powder in 1L of distilled water to prepare solution A with the concentration of 0.1 g/ml;
the second step is that: dissolving 100g protease in 1L distilled water to obtain solution B with pH of 7.0 and concentration of 0.1 g/ml;
the third step: 10g of trehalose powder, 10g of hyaluronic acid according to 1: dissolving 1L of distilled water in the proportion of 1 to prepare a C solution with the concentration of 0.01 g/ml;
the fourth step: heating the solution A to 50 ℃, adding the solution B (the ratio of enzyme to protein is 1: 100) according to the proportion of 1/100 of the solution A to maintain the temperature, and testing the pH value to be 7, wherein the pH value is maintained at 7 by using NAHO if the conditions are changed;
the fifth step: after reacting for two hours, heating the solution to 80-100 ℃ to destroy the protease, stopping the hydrolysis reaction of the enzyme, cooling to room temperature, and adding 1/10C solution for stabilization.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111048653.3A CN113633568A (en) | 2021-09-08 | 2021-09-08 | Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111048653.3A CN113633568A (en) | 2021-09-08 | 2021-09-08 | Preparation method of antioxidant and anti-aging stock solution of incomplete enzyme digestion sericin |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101596311A (en) * | 2008-06-02 | 2009-12-09 | 上海万兴生物制药有限公司 | Stable kallikrein-1 injection |
| CN103462867A (en) * | 2013-09-05 | 2013-12-25 | 浙江大学 | Preparation method of silk glue/trehalose skin-care facial mask |
| CN105331661A (en) * | 2015-11-24 | 2016-02-17 | 浙江汇能生物股份有限公司 | Method for preparing sericin polypeptides with aqueous enzymatic method |
| CN110499350A (en) * | 2019-03-15 | 2019-11-26 | 辽东学院 | A kind of sericin method for hydrolysis and products thereof and application |
-
2021
- 2021-09-08 CN CN202111048653.3A patent/CN113633568A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101596311A (en) * | 2008-06-02 | 2009-12-09 | 上海万兴生物制药有限公司 | Stable kallikrein-1 injection |
| CN103462867A (en) * | 2013-09-05 | 2013-12-25 | 浙江大学 | Preparation method of silk glue/trehalose skin-care facial mask |
| CN105331661A (en) * | 2015-11-24 | 2016-02-17 | 浙江汇能生物股份有限公司 | Method for preparing sericin polypeptides with aqueous enzymatic method |
| CN110499350A (en) * | 2019-03-15 | 2019-11-26 | 辽东学院 | A kind of sericin method for hydrolysis and products thereof and application |
Non-Patent Citations (3)
| Title |
|---|
| 张克勤等: "《文化丝绸》", 31 August 2016, 苏州大学出版社 * |
| 张玉华: "海藻糖和透明质酸对生物分子和微生物的保护及其作用机制", 《万方数据库》 * |
| 糖酒快讯息: "海藻糖的功效与作用（海藻糖有什么功效作用）", 《HTTPS://WWW.SPZS.COM/BAIKE/174234.HTML》 * |
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Application publication date: 20211112 |