CN103829029A - Production method of non-denatured pure sericin stock solution - Google Patents
Production method of non-denatured pure sericin stock solution Download PDFInfo
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- CN103829029A CN103829029A CN201410087212.8A CN201410087212A CN103829029A CN 103829029 A CN103829029 A CN 103829029A CN 201410087212 A CN201410087212 A CN 201410087212A CN 103829029 A CN103829029 A CN 103829029A
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Abstract
The invention discloses a production method of a non-denatured pure sericin stock solution. By utilizing the production method, the pure sericin stock solution is produced by evading sericin denatured conditions. The production method is simple, low in cost and suitable for production of nutritious foods, health care products and products in other fields.
Description
Technical field
The present invention relates generally to production field, relates in particular to a kind of without sex change pure silk glue protein stoste production method.
Background technology
Sericine is ease of solubility globulin, holds easily absorbed by the body; Be rich in 18 kinds of essential amino acids of the mankind and be rich in many carboxyls, natural moisture preserving composition, water-retaining property is strong, play moisturizing, wrinkle resistant, whitening function; Easily form chelate with the heavy metal such as copper, mercury, restraint of tyrosinase isoreactivity, contains and removes free radical functional group, and digestibility is low, plays anti-oxidantly, plays and keeps food security, reduces the active function of digestive system canceration material etc.Therefore sericin has as the functional material use aspect of nutraceutical, healthcare products and other field many unique functions and larger market potential.
The acquisition of the existing sericine of China, reclaims the waste liquid of mainly taking to get rid of from throwing factory.Waste liquid reclaims sericine, and not only technique is numerous and diverse, efficiency is low, cost is high, seriously polluted, and the sericine low-purity reclaiming, strong sex change do not reach quality requirement in the application of the numerous areas relevant to human body.
The sericine of sex change has a strong impact on the performance of its active function.The sex change of sericine occurs in certain temperature range, and temperature is higher, sex change is stronger, and sex change very easily occurs Long contact time high temperature, both may lose solubility originally, also can lose physiologically active.In the time of low temperature a little less than, generally there is not denaturation; There are some researches prove sex change can not occur in 24 hours at 60 ℃.
The activity of sericine occurs within the scope of certain pH, and too high, too low sericine sex change is stronger.There are some researches prove that sex change can not occur pH between 6-8.
Nowadays the sericine extensively utilizing is to reclaim from the Silk Reeling Waste Liquor of common silk cocoon.Although can be salvaged, but there is shortcomings: 1. the common silk cocoon of filature will be through the post bake cocoon drying that 90 ℃ of heads dry, 110 ℃ two is dried, the heating cocoon cooking of 95 ℃, the heating filature of 85 ℃, from then in the waste liquid producing, reclaim sericine, the sericine sex change that the high temperature that is difficult to avoid causes.2. common silk cocoon cocoon shell itself only contains the sericine of 25% left and right, and reclaims from a large amount of silk reeling wastewaters, and organic efficiency is low, cost is high; 3. by common silk cocoon to reclaiming Silk Reeling Waste Liquor sericine, many time of program of process is long, technique is numerous and diverse, is difficult to remove the impurity that mixes and putrefaction and the chemical residual of participation filature on reclaimer operation, the sericine purity of recovery is lower.From cost performance angle, be unprofitable; From security standpoint, being applied to the cosmetic directly related with human body, health care, field of medicaments does not only have enough assurances qualitatively, yet may have larger hidden danger.
Summary of the invention
The object of the invention is exactly in order to provide a kind of without sex change pure silk glue protein stoste production method.
The present invention is achieved by the following technical solutions:
A kind of without sex change pure silk glue protein stoste production method, comprise the following steps:
(1) Introduced from Japan silk gum kind " silk gum star ", raises production technology with routine and produces, and obtains All Pure Nature without fibroin pure silk glue silk cocoon;
(2) artificial directly hand tears the silk gum cocoon shell of peeling off silk cocoon;
(3), at 60-65 ℃ of silk gum cocoon shell constant temperature blast drier 8-12min, rub by hand broken silk gum cocoon shell;
(4) under 60-65 ℃ of condition, with pulverizer, above-mentioned silk gum cocoon shell is ground into 150-200 object silk gum powder;
(5) above-mentioned silk gum powder is joined in the aqueous sodium carbonate of its weight 95-105 0.5-1% doubly, in 60-70 ℃ of thermostat water bath, dissolve 25-35min;
(6) the silk gum solution after above-mentioned water bath processing is crossed to 150-200 object membrane filtration and remove impurity, obtain pure sericine stoste.
A kind of without sex change pure silk glue protein stoste, according to nutraceutical, healthcare products and other field production needs, the directly sericine stoste of preparation variable concentrations, or adopt freeze-drying method to prepare without the pure sericin powder of sex change, be diluted with water into the sericine stoste of variable concentrations requirement.
Advantage of the present invention is:
Method of the present invention is evaded sericine Denaturing and is produced the pure stoste of sericine, and method is simple, with low cost, is applicable to nutraceutical, healthcare products and other field production.
The specific embodiment
Embodiment 1
A kind of without sex change pure silk glue protein stoste production method, comprise the following steps:
(1) Introduced from Japan silk gum kind " silk gum star ", raises production technology with routine and produces, and obtains All Pure Nature without fibroin pure silk glue silk cocoon;
(2) artificial directly hand tears the silk gum cocoon shell of peeling off silk cocoon;
(3), at 65 ℃ of silk gum cocoon shell constant temperature blast drier 10min, rub by hand broken silk gum cocoon shell;
(4) under 60 ℃ of conditions, with pulverizer, above-mentioned silk gum cocoon shell is ground into 150-200 object silk gum powder;
(5) above-mentioned silk gum powder is joined in 0.5% the aqueous sodium carbonate of 100 times, in 65 ℃ of thermostat water baths, dissolve 30min;
(6) the silk gum solution after above-mentioned water bath processing is crossed to 150-200 object membrane filtration and remove impurity, obtain pure sericine stoste.
A kind of without sex change pure silk glue protein stoste, according to nutraceutical, healthcare products and other field production needs, the directly sericine stoste of preparation variable concentrations, or adopt freeze-drying method to prepare without the pure sericin powder of sex change, be diluted with water into the sericine stoste of variable concentrations requirement.
Claims (2)
1. without a sex change pure silk glue protein stoste production method, it is characterized in that comprising the following steps:
(1) Introduced from Japan silk gum kind " silk gum star ", raises production technology with routine and produces, and obtains All Pure Nature without fibroin pure silk glue silk cocoon;
(2) artificial directly hand tears the silk gum cocoon shell of peeling off silk cocoon;
(3), at 60-65 ℃ of silk gum cocoon shell constant temperature blast drier 8-12min, rub by hand broken silk gum cocoon shell;
(4) under 60-65 ℃ of condition, with pulverizer, above-mentioned silk gum cocoon shell is ground into 150-200 object silk gum powder;
(5) above-mentioned silk gum powder is joined in the aqueous sodium carbonate that its weight 95-105 concentration is doubly 0.5-1%, in 60-70 ℃ of thermostat water bath, dissolve 25-35min;
(6) the silk gum solution after above-mentioned water bath processing is crossed to 150-200 object membrane filtration and remove impurity, obtain pure sericine stoste.
2. according to claim 1 without sex change pure silk glue protein stoste, it is characterized in that according to nutraceutical, healthcare products and other field production needs, the directly sericine stoste of preparation variable concentrations, or adopt freeze-drying method to prepare without the pure sericin powder of sex change, be diluted with water into the sericine stoste of variable concentrations requirement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410087212.8A CN103829029A (en) | 2014-03-11 | 2014-03-11 | Production method of non-denatured pure sericin stock solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410087212.8A CN103829029A (en) | 2014-03-11 | 2014-03-11 | Production method of non-denatured pure sericin stock solution |
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| CN103829029A true CN103829029A (en) | 2014-06-04 |
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| CN201410087212.8A Pending CN103829029A (en) | 2014-03-11 | 2014-03-11 | Production method of non-denatured pure sericin stock solution |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107802585A (en) * | 2017-12-14 | 2018-03-16 | 佛山市万良商贸有限公司 | A kind of silk gum condensation and preparation method thereof |
| CN107802584A (en) * | 2017-12-14 | 2018-03-16 | 佛山市万良商贸有限公司 | A kind of silk gum eye gel and preparation method thereof |
| CN107823102A (en) * | 2017-12-14 | 2018-03-23 | 佛山市万良商贸有限公司 | A kind of silk gum moisturiser and preparation method thereof |
| CN107929187A (en) * | 2017-12-14 | 2018-04-20 | 佛山市万良商贸有限公司 | A kind of sericin facial mask essence and preparation method thereof |
| CN107937460A (en) * | 2016-10-13 | 2018-04-20 | 宜宾屏山辉瑞油脂有限公司 | A kind of preparation method for hydrolyzing sericin peptide taken |
| CN107998019A (en) * | 2017-12-14 | 2018-05-08 | 佛山市万良商贸有限公司 | A kind of silk gum lotion and preparation method thereof |
| CN113372426A (en) * | 2020-03-10 | 2021-09-10 | 森田生医股份有限公司 | Sericin extract, preparation method and use thereof, and antioxidant composition comprising the same |
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| CN1407992A (en) * | 1999-12-09 | 2003-04-02 | 独立行政法人农业生物资源研究所 | Process for producing functional silk fibroin and utilization of the same |
| CN101401563A (en) * | 2008-11-07 | 2009-04-08 | 安徽省农业科学院蚕桑研究所 | New silkworm breed breeding method for mass-production of full-natural pure sericin protein |
| CN101856318A (en) * | 2010-04-27 | 2010-10-13 | 浙江省农业科学院 | Preparation method of pure-natural, high-purity and invariable green sericin powder |
| CN102961298A (en) * | 2012-12-21 | 2013-03-13 | 李卫旗 | Manufacturing method of body lotion by taking Pullulan polysaccharide and sericin bioactive peptide as main raw materials |
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2014
- 2014-03-11 CN CN201410087212.8A patent/CN103829029A/en active Pending
Patent Citations (4)
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| CN1407992A (en) * | 1999-12-09 | 2003-04-02 | 独立行政法人农业生物资源研究所 | Process for producing functional silk fibroin and utilization of the same |
| CN101401563A (en) * | 2008-11-07 | 2009-04-08 | 安徽省农业科学院蚕桑研究所 | New silkworm breed breeding method for mass-production of full-natural pure sericin protein |
| CN101856318A (en) * | 2010-04-27 | 2010-10-13 | 浙江省农业科学院 | Preparation method of pure-natural, high-purity and invariable green sericin powder |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937460A (en) * | 2016-10-13 | 2018-04-20 | 宜宾屏山辉瑞油脂有限公司 | A kind of preparation method for hydrolyzing sericin peptide taken |
| CN107937460B (en) * | 2016-10-13 | 2020-08-14 | 宜宾屏山辉瑞科技有限公司 | Preparation method of hydrolyzed sericin peptide |
| CN107802585A (en) * | 2017-12-14 | 2018-03-16 | 佛山市万良商贸有限公司 | A kind of silk gum condensation and preparation method thereof |
| CN107802584A (en) * | 2017-12-14 | 2018-03-16 | 佛山市万良商贸有限公司 | A kind of silk gum eye gel and preparation method thereof |
| CN107823102A (en) * | 2017-12-14 | 2018-03-23 | 佛山市万良商贸有限公司 | A kind of silk gum moisturiser and preparation method thereof |
| CN107929187A (en) * | 2017-12-14 | 2018-04-20 | 佛山市万良商贸有限公司 | A kind of sericin facial mask essence and preparation method thereof |
| CN107998019A (en) * | 2017-12-14 | 2018-05-08 | 佛山市万良商贸有限公司 | A kind of silk gum lotion and preparation method thereof |
| CN113372426A (en) * | 2020-03-10 | 2021-09-10 | 森田生医股份有限公司 | Sericin extract, preparation method and use thereof, and antioxidant composition comprising the same |
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Application publication date: 20140604 |