CN102961298A - Manufacturing method of body lotion by taking Pullulan polysaccharide and sericin bioactive peptide as main raw materials - Google Patents
Manufacturing method of body lotion by taking Pullulan polysaccharide and sericin bioactive peptide as main raw materials Download PDFInfo
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- CN102961298A CN102961298A CN2012105614814A CN201210561481A CN102961298A CN 102961298 A CN102961298 A CN 102961298A CN 2012105614814 A CN2012105614814 A CN 2012105614814A CN 201210561481 A CN201210561481 A CN 201210561481A CN 102961298 A CN102961298 A CN 102961298A
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Abstract
The invention provides a manufacturing method of body lotion by taking Pullulan polysaccharide and sericin bioactive peptide as main raw materials. According to the method, the body lotion is manufactured by using the Pullulan polysaccharide and the sericin bioactive peptide which are taken as main components and other auxiliary components in a matching way. The manufacturing method comprises the following steps of: 1. carrying out alkaline solution eluting and primary hydrolysis on sericin proteins; 2. separating and concentrating sericin protein liquid; 3. carrying out ultrasonic auxiliary enzymolysis on the sericin proteins; 4. separating the sericin bioactive peptide; 5. dissolving the Pullulan polysaccharide; and 6. preparing the body lotion by taking the Pullulan polysaccharide and the sericin bioactive peptide as the main raw materials according to the components. The molecular weight of the sericin bioactive peptide formed by using hydrolysis is in a range of 5000-20000Da; the sericin bioactive peptide is more easily absorbed by the skin than macromolecular proteins; and the effect of the sericin bioactive peptide is far better than that of the traditional collagen. The raw materials of the sericin bioactive peptide are selected from cocoon shells abandoned during a silk industry; and a secondary resource is effectively utilized and the production cost is greatly reduced.
Description
Technical field
The present invention relates to a kind of production technology of moisturizing active white skin care solution, particularly a kind of with the blue polysaccharide in general Shandong and silk
The glue bioactive peptide is the skin care liquid manufacture method of primary raw material, belongs to the skin care item production technical field.
Background technology
Bioactive polysaccharide is an important directions of biological beauty as the application of effect components of cosmetics in cosmetics.At present, the polysaccharide such as hyaluronic acid, chitin and heparin have a wide range of applications at cosmetic industry as effect type wetting agent.The blue polysaccharide in general Shandong is again Pul, it is the outer biological polysaccharide of born of the same parents of the fermentation generation of Aureobasidium pullulans, because physical chemistry and biological characteristics that this polysaccharide has many uniquenesses, nontoxic, viscosity is low, plasticity is strong, good film-forming property, preferably water solublity, moisture absorbability and moisture retentivity and antibiotic, bacteria resistance function are arranged, it can not only produce moisturizing, the stable colour of skin, defying age and the effect such as antibiotic improved, and can also dispel heavy metal ion superfluous in the skin.Therefore, the blue polysaccharide in general Shandong has wide prospect as effect type cosmetics additive, is a kind of Multifucntional biological product with good economic worth.
Biologically active polypeptide is few at people's in-vivo content, but biological activity is high, and " desquamation " process of skin is carried out smoothly, makes dead bark be unlikely aging accumulation and obstruction pore, makes constantly taking on a new look of skin, is the important component in the skin care item.
In Mulberry cocoon industry, a large amount of cocoon shells, husks and filature water often are used as refuse and abandon, the natural silk glue protein that is rich in these materials, have moisturizing, nutrition, skin care effect and anti-oxidation function, use with sericin liquid can keep whitening skin, smooth high resilience as the sericin skin care milk of key component.The molecular conformation of water-soluble silk glue albumen is the random coil type, the space is loose unordered, make the many polarity hydrophilic groups on the polypeptide chain of sericin be in the polypeptide surface, it can make moisture transmission in the structure to keratodermatitis and with its combination, therefore sericin is a kind of natural Moisture factor, when extraneous weather changes, plays damping, moisture-keeping function, can make skin keep certain moisture, to prevent xerosis cutis, pruritus; Sericin also has the effect of restraint of tyrosinase activity, and then reaches the generation that suppresses dermal melanin; Sericin also has anti-oxidation function, can resist the ultraviolet in the sunlight, and skin darkening produces mottle after the prevention Exposure to Sunlight, and cell and protein are had the freeze protection effect; Aminoacid in the sericin absorbs through skin, can play the effect that replenishes skin-nourishing, makes skin keep pale, soft, high resilience.
Yet the sericin molecular size range without degraded differs, and has part to be difficult to be absorbed by the skin, must be through resolution process, and being micromolecule polypeptide could be absorbed by human body.Micromolecule polypeptide can directly be absorbed by the skin; be about 5000-20000 Da as good take mean molecule quantity in the cosmetics; can effectively improve hydrophilic and the protection horny layer on coarse skin surface; can also produce skin wet, level and smooth effect; can be used for removing the various mottles of human body face skin, acne; the protection skin elasticity is dispelled face skin wrinkle, delays the skin of face aging.
Summary of the invention
The objective of the invention is by with specific technique hydrolyzed-silk glue protein from silkworm cocoon, directional decomposition obtains the sericin bioactive peptide that the molecular weight section is 5000-20000 Da, and utilize the blue polysaccharide in general Shandong and sericin bioactive peptide to be main component, be equipped with other auxiliary element, produce cheap but the novel skin care liquid product that the moisturizing white-skinned face function is good of a kind of cost of material.
The present invention is achieved in that a kind of blue polysaccharide and sericin bioactive peptide are the skin care liquid manufacture method of primary raw material take general Shandong:
1. the aqueous alkali eluting of sericin and just one-step hydrolysis: take by weighing a certain amount of cocoon shell (100-500g), simply pulverizing makes it be the square fragment of 1-2mm on pulverizer, then measures the distilled water of 20-50 times of quality of cocoon shell, is mixed with to contain NaHCO
3Mass fraction is the aqueous slkali of 2-10% concentration, cocoon shell fragment be impregnated in the aqueous solution, make the abundant swelling 10-30min of husks, simultaneously the ultrasonic generator probe is inserted below the aqueous solution water surface, apart from container bottom 5-15cm, power is 150-250W, acts on 40-90min under 50-80 ℃ of temperature, sericin in the cocoon shell fully is eluted in the solution, and to a certain extent hydrolysis.
2. the separation of sericin liquid is with concentrated: undissolvable silkworm silk residue is pulled out, and with a small amount of distilled water wash 2-5 time, and washings are merged in the hydrolyzed solution.Silkworm silk is dried to constant weight under 50-70 ℃ of temperature, and weighing silkworm silk deducts silkworm silk weight with cocoon shell original weight, can calculate the sericin quality of dissolving in the eluent.The sericin hydrolyzed solution is heated the 1/10-1/5 that is concentrated into original volume under 60-90 ℃ of temperature, obtain the concentrated solution of sericin.
3. the ultrasound-assisted enzymolysis of sericin: the 0.5-2.0% trypsin that adds the sericin quality in the concentrated solution of sericin carries out enzymolysis, hydrolysis temperature is 35-39 ℃, pH value is 7.6-8.7, ultrasonic generator power is 150-250W, behind the effect 20-50min, stop ultrasound wave, continue enzymolysis 60-120min
Make the sericin in the solution be hydrolyzed to the sericin bioactive peptide that mean molecule quantity is 5000-20000Da.Then solution is heated to 100 ℃ and keeps 10-30min, enzyme denaturing is lived.
4. the separation of sericin bioactive peptide: with the sericin bioactive peptide solution behind the enzyme denaturing through activated carbon filtration, remove pigment, heating is concentrated into the 1/4-1/2 of original volume under 60-90 ℃ of temperature, after naturally cooling to room temperature, be the bag filter flowing water dialysis 24-36h of 2000-5000Da with molecular cut off, the micromolecule inorganic matter dialysis that remains in the sericin bioactive peptide solution is got rid of, then in sericin bioactive peptide solution, add 95% ethanol with volume, fully mix 5-30 min, make sericin bioactive peptide flocculation sediment, sedimentation and filtration is collected, and vacuum drying is to constant weight.
5. the dissolving of the blue polysaccharide in general Shandong: the blue polysaccharide pressed powder in general Shandong is dissolved in the deionized water of 10-20 times of weight, and abundant stirring and dissolving under 50-80 ℃ of temperature is not until precipitate.
One kind take general Shandong blue polysaccharide and sericin bioactive peptide as the skin care liquid of primary raw material formulated by following component (%/w):
The blue polysaccharide 2.0-5.0 in general Shandong, sericin bioactive peptide 1.0-5.5, butylene glycol 3.0-7.0, vitamin E acetate mixture 1.0-2.5, glycerol 1.0-2.0, oil-soluble pelargonium roseum 0.1-0.3, oat beta-glucan 0.2-0.7, antiseptic methyl isothiazoline 0.1-0.4, Jojoba oil 1.5-2.5, deionized water adds to 100.
The present invention compares with similar products, has following characteristics:
1. in moisturizing Skin whitening care cosmetics commonly used, hyaluronic acid and collagen protein are topmost moisturizing whitening compositions.In the product of the present invention, replaced hyaluronic acid with the blue polysaccharide in general Shandong, the blue polysaccharide in general Shandong has good water solublity, dispersibility, film property, hygroscopicity and nonhazardous, can produce in enormous quantities by microbial fermentation, only be hyaluronic 1/10th in price, but its effect is very nearly the same with hyaluronic acid.Simultaneously replaced collagen protein with the sericin bioactive peptide, because traditional cosmetics collagen protein is taken from the animal proteinums such as domestic animal Fish more, complicated components, function difference is large, and the skin protection performance lacks specific aim.And sericin has certain effects aspect skin care, and the molecular weight of the sericin bioactive peptide that is formed by its hydrolysis is absorbed by skin than high molecular weight protein is easier in the 5000-20000Da scope, and effect far surpasses traditional collagen protein.
2. in the processing technology of sericin bioactive peptide, adopted under the ultrasound wave subsidiary conditions, the cocoon shell material is carried out the alkali liquor eluting hydrolysis of sericin, the segment processing of protease hydrolysis behind the dialysis eluting, directional decomposition and obtain the sericin bioactive peptide of 5000-20000 Da molecular weight sections, obtain the sericin bioactive peptide of the strongest active molecular weight section, both improved product quality than single acid and alkali hydrolysis mode, improved efficient than plurality of enzymes Hydrolyze method again.
3. the raw material of sericin bioactive peptide comes from the discarded cocoon shell of silkworm silk industry, has effectively utilized secondary resource, and has greatly reduced production cost.
The specific embodiment
Take by weighing the cocoon shell of dry weight 300g, simply pulverizing makes it be the square fragment of 1-2mm on pulverizer, then measures the distilled water of 10L, is mixed with to contain NaHCO
3Mass fraction is the aqueous slkali of 5% concentration, cocoon shell fragment be impregnated in the aqueous solution, make the abundant swelling 20min of husks, simultaneously the ultrasonic generator probe is inserted below the aqueous solution water surface, apart from container bottom 10cm, power is 200W, acts on 60min under 75 ℃ of temperature, sericin in the cocoon shell fully is eluted in the solution, and to a certain extent hydrolysis.
Undissolvable silkworm silk residue is pulled out, used the 200ml distilled water wash, repeat 3 times, washings are merged in the hydrolyzed solution.The silkworm silk oven dry is dried to constant weight under 60 ℃ of temperature, and weighing silkworm silk heavily is 173g, and cocoon shell original weight is deducted silk quality, can calculate sericin quality 127 g that dissolve in the eluent.The sericin hydrolyzed solution heated under 80 ℃ of temperature be concentrated into 1.5L, obtain the concentrated solution of sericin.
In the concentrated solution of above-mentioned sericin, add 1.5 g trypsin and carry out enzymolysis, hydrolysis temperature is 37 ℃, pH value is 8.0, ultrasonic generator power is 200W, behind the effect 40min, stop ultrasound wave, continue enzymolysis 100min, make the sericin in the solution be hydrolyzed to the sericin bioactive peptide that mean molecule quantity is 5000-20000Da.Then solution is heated to 100 ℃ and keeps 20min, enzyme denaturing is lived.
With the sericin bioactive peptide solution behind the enzyme denaturing through activated carbon filtration, remove pigment, heating is concentrated into 600 L of original volume under 80 ℃ of temperature, after naturally cooling to room temperature, be the bag filter flowing water dialysis 36h of 3000Da with molecular cut off, the micromolecule inorganic matter dialysis that remains in sericin bioactive peptide solution is got rid of, then in sericin bioactive peptide solution, add 95% ethanol with volume, fully mix 20 min, make sericin bioactive peptide flocculation sediment, sedimentation and filtration is collected, and vacuum drying gets sericin bioactive peptide 96 g to constant weight.
The dissolving of the blue polysaccharide in general Shandong: the blue polysaccharide pressed powder in general Shandong is dissolved in the deionized water of 20 times of weight, and abundant stirring and dissolving under 60 ℃ of temperature is not until precipitate.
The charge ratio of making the blue polysaccharide skin care liquid in the general Shandong of 100 g finished product sericin bioactive peptide is:
Blue polysaccharide 5.0 g in general Shandong, sericin bioactive peptide 3.0 g, butylene glycol 4.0 g, vitamin E acetate mixture 1.5 g, glycerol 1.5 g, oil-soluble pelargonium roseum 0.2 g, oat beta-glucan 0.6 g, antiseptic methyl isothiazoline 0.2 g, Jojoba oil 2.0 g, deionized water adds to 100 g.
Claims (1)
- One kind take general Shandong blue polysaccharide and sericin bioactive peptide is characterized in that as the skin care liquid manufacture method of primary raw material:1) the aqueous alkali eluting of sericin and first one-step hydrolysis: take by weighing a certain amount of cocoon shell (100-500g), simply pulverizing makes it be the square fragment of 1-2mm on pulverizer, then measures the distilled water of 20-50 times of quality of cocoon shell, is mixed with to contain NaHCO 3Mass fraction is the aqueous slkali of 2-10% concentration, cocoon shell fragment be impregnated in the aqueous solution, make the abundant swelling 10-30min of husks, simultaneously the ultrasonic generator probe is inserted below the aqueous solution water surface, apart from container bottom 5-15cm, power is 150-250W, acts on 40-90min under 50-80 ℃ of temperature, sericin in the cocoon shell fully is eluted in the solution, and to a certain extent hydrolysis;2) separation of sericin liquid is with concentrated: undissolvable silkworm silk residue is pulled out, and with a small amount of distilled water wash 2-5 time, and washings are merged in the hydrolyzed solution, silkworm silk is dried to constant weight under 50-70 ℃ of temperature, weighing silkworm silk deducts silkworm silk weight with cocoon shell original weight, can calculate the sericin quality of dissolving in the eluent, the sericin hydrolyzed solution is heated the 1/10-1/5 that is concentrated into original volume under 60-90 ℃ of temperature, obtain the concentrated solution of sericin;3) ultrasound-assisted enzymolysis of sericin: the 0.5-2.0% trypsin that adds the sericin quality in the concentrated solution of sericin carries out enzymolysis, hydrolysis temperature is 35-39 ℃, pH value is 7.6-8.7, ultrasonic generator power is 150-250W, behind the effect 20-50min, stop ultrasound wave, continue enzymolysis 60-120minMake the sericin in the solution be hydrolyzed to the sericin bioactive peptide that mean molecule quantity is 5000-20000Da, then solution is heated to 100 ℃ and keeps 10-30min, enzyme denaturing is lived;4) separation of sericin bioactive peptide: with the sericin bioactive peptide solution behind the enzyme denaturing through activated carbon filtration, remove pigment, heating is concentrated into the 1/4-1/2 of original volume under 60-90 ℃ of temperature, after naturally cooling to room temperature, be the bag filter flowing water dialysis 24-36h of 2000-5000Da with molecular cut off, the micromolecule inorganic matter dialysis that remains in the sericin bioactive peptide solution is got rid of, then in sericin bioactive peptide solution, add 95% ethanol with volume, fully mix 5-30 min, make sericin bioactive peptide flocculation sediment, sedimentation and filtration is collected, and vacuum drying is to constant weight;5) dissolving of the blue polysaccharide in general Shandong: the blue polysaccharide pressed powder in general Shandong is dissolved in the deionized water of 10-20 times of weight, and abundant stirring and dissolving under 50-80 ℃ of temperature is not until precipitate;6) a kind of take general Shandong blue polysaccharide and sericin bioactive peptide as the skin care liquid of primary raw material formulated by following component (%/w):The blue polysaccharide 2.0-5.0 in general Shandong, sericin bioactive peptide 1.0-5.5, butylene glycol 3.0-7.0, vitamin E acetate mixture 1.0-2.5, glycerol 1.0-2.0, oil-soluble pelargonium roseum 0.1-0.3, oat beta-glucan 0.2-0.7, antiseptic methyl isothiazoline 0.1-0.4, Jojoba oil 1.5-2.5, deionized water adds to 100.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103830119A (en) * | 2014-03-11 | 2014-06-04 | 安徽省农业科学院蚕桑研究所 | Preparation method of toning lotion taking natural sericin as matrix |
| CN103829029A (en) * | 2014-03-11 | 2014-06-04 | 安徽省农业科学院蚕桑研究所 | Production method of non-denatured pure sericin stock solution |
| CN104546649A (en) * | 2014-12-29 | 2015-04-29 | 无锡蚕魅科技有限公司 | Sun-proof repair liquid containing silk extractive and preparation method thereof |
| CN104586725A (en) * | 2014-12-29 | 2015-05-06 | 无锡蚕魅科技有限公司 | Sunscreen gel containing silk extraction solution and preparation method of sunscreen gel |
| CN104825349A (en) * | 2015-04-21 | 2015-08-12 | 浙江大学湖州市南太湖现代农业科技推广中心 | Method for producing kin care gel application agent from biomass raw material |
| CN104861178A (en) * | 2015-05-26 | 2015-08-26 | 西北大学 | Pullulan-hyaluronic acid hydrogel and preparation method thereof |
| CN106539700A (en) * | 2016-11-25 | 2017-03-29 | 江苏爱西施科技服务咨询股份有限公司 | A kind of Firm defying age silk peptide facial treatment milk and preparation method thereof |
| CN107164439A (en) * | 2016-09-20 | 2017-09-15 | 国家海洋局第三海洋研究所 | A kind of large-scale preparation method of fish scale NTx protein peptides suitable for cosmetics |
| CN108997942A (en) * | 2018-07-26 | 2018-12-14 | 苏州华龙化工有限公司 | A kind of preparation method of sericin grape pip polysaccharide composite leather gloss agent |
| CN112515980A (en) * | 2019-09-18 | 2021-03-19 | 株式会社Lg生活健康 | Cosmetic composition comprising high molecular weight sericin |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103830119A (en) * | 2014-03-11 | 2014-06-04 | 安徽省农业科学院蚕桑研究所 | Preparation method of toning lotion taking natural sericin as matrix |
| CN103829029A (en) * | 2014-03-11 | 2014-06-04 | 安徽省农业科学院蚕桑研究所 | Production method of non-denatured pure sericin stock solution |
| CN103830119B (en) * | 2014-03-11 | 2016-03-23 | 安徽省农业科学院蚕桑研究所 | A kind of with the preparation method of the natural silk glue protein astringent that is substrate |
| CN104546649A (en) * | 2014-12-29 | 2015-04-29 | 无锡蚕魅科技有限公司 | Sun-proof repair liquid containing silk extractive and preparation method thereof |
| CN104586725A (en) * | 2014-12-29 | 2015-05-06 | 无锡蚕魅科技有限公司 | Sunscreen gel containing silk extraction solution and preparation method of sunscreen gel |
| CN104825349A (en) * | 2015-04-21 | 2015-08-12 | 浙江大学湖州市南太湖现代农业科技推广中心 | Method for producing kin care gel application agent from biomass raw material |
| CN104861178A (en) * | 2015-05-26 | 2015-08-26 | 西北大学 | Pullulan-hyaluronic acid hydrogel and preparation method thereof |
| CN107164439A (en) * | 2016-09-20 | 2017-09-15 | 国家海洋局第三海洋研究所 | A kind of large-scale preparation method of fish scale NTx protein peptides suitable for cosmetics |
| CN106539700A (en) * | 2016-11-25 | 2017-03-29 | 江苏爱西施科技服务咨询股份有限公司 | A kind of Firm defying age silk peptide facial treatment milk and preparation method thereof |
| CN108997942A (en) * | 2018-07-26 | 2018-12-14 | 苏州华龙化工有限公司 | A kind of preparation method of sericin grape pip polysaccharide composite leather gloss agent |
| CN112515980A (en) * | 2019-09-18 | 2021-03-19 | 株式会社Lg生活健康 | Cosmetic composition comprising high molecular weight sericin |
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Application publication date: 20130313 |