Summary of the invention
In order to solve to solve above-mentioned technical problem, the invention provides that a kind of irritated rate is low, active component compared with limits reservation and be easy to repair condensation for people stops after absorbed Pupa bombycis bioactive peptide shines.
Present invention also offers the preparation method of repairing condensation after described Pupa bombycis bioactive peptide shines.
The present invention is achieved by the following measures:
Repair condensation after a kind of Pupa bombycis active polypeptide shines, the percetage by weight of raw material is as follows:
Pupa bombycis active polypeptide 5%-15%,
Herba portulacae extract 1%-3%,
Megranate Seed P.E 1%-3%,
Tremella polysaccharide 4%-10%,
Flos Rosae Rugosae quintessence oil 1%-2%,
Nisin 0.05%-0.1%,
Gel-type vehicle 0.2%-1.5%,
Wetting agent 8%-15%,
Penetrating agent 0.5%-1%,
Triethanolamine 0.5%-1.5%,
Disodiumedetate 0.01%-0.05%,
Antiseptic 0.05%-0.1%,
Surplus is pure water or normal saline.
Described Pupa bombycis active polypeptide repairs condensation after shining, and the percetage by weight of raw material is as follows:
Pupa bombycis active polypeptide 8%-13%,
Herba portulacae extract 1%-2%,
Megranate Seed P.E 1%-2%,
Tremella polysaccharide 5%-8%,
Flos Rosae Rugosae quintessence oil 1%-2%,
Nisin 0.05%-0.08%,
Gel-type vehicle 0.5%-1%,
Wetting agent 8%-15%,
Penetrating agent 0.5%-1%,
Triethanolamine 0.5%-1.5%,
Disodiumedetate 0.02%-0.04%,
Antiseptic 0.05%-0.1%,
Surplus is pure water or normal saline.
Described Pupa bombycis active polypeptide repairs condensation after shining, and the percetage by weight of raw material is as follows:
Pupa bombycis active polypeptide 13%,
Herba portulacae extract 1%,
Megranate Seed P.E 1%,
Tremella polysaccharide 5%,
Flos Rosae Rugosae quintessence oil 1%,
Nisin 0.05%,
Gel-type vehicle 1%,
Wetting agent 8%,
Penetrating agent 0.5%,
Triethanolamine 0.5%,
Disodiumedetate 0.02%,
Antiseptic 0.05%,
Surplus is pure water or normal saline.
The Herba portulacae extract of above-mentioned use, Megranate Seed P.E are alcohol extract.
Described Pupa bombycis active polypeptide repairs condensation after shining, and preferred Pupa bombycis active polypeptide is obtained by following steps:
(1) prepare pupa albumen aqueous solution, add papain enzymolysis, enzyme concentration 8000-60000IU/g pupa albumen, enzymolysis terminates rear enzyme denaturing and obtains enzymolysis solution;
(2) regulate enzymolysis solution pH to 8.0 ~ 11.0, add Esperase protease, enzyme concentration 0.075-0.225KNPU/g pupa albumen, enzymolysis;
(3) cross after the product enzyme denaturing of step (2) and filter insoluble matter, filtrate crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) filtrate is through decolouring, desalination, concentrated, dry, obtains silkworm pupa protein polypeptide.
Hydrolysis temperature 45 ~ 60 DEG C in preferred steps (1), pH value 6.5 ~ 8.0, enzymolysis time 2 ~ 4h.
Hydrolysis temperature 45 DEG C ~ 60 DEG C in preferred steps (2), time 2 ~ 4h.
Described Pupa bombycis active polypeptide skin rejuvenating facial mask,
Described gel-type vehicle is more than one in Acritamer 940, sodium carboxymethyl cellulose and glycan substrate;
Described wetting agent is more than one in glycerol, hyaluronic acid, Trivent OCG, glucosan glycosides, poly-glycosaminoglycan and lecithin;
Described penetrating agent is more than one in azone and dextran sulfate;
Described antiseptic is more than one in methyl parahydroxybenzoate, propyl p-hydroxybenzoate, isopropyl alcohol and propylene glycol.
Repair the preparation method of condensation after described Pupa bombycis active polypeptide shines, comprise the steps:
(1) wetting agent, penetrating agent, disodiumedetate, antiseptic are added in container, add pure water or normal saline, stirring and dissolving, then gel-type vehicle is added, continue to be stirred to gel-type vehicle evenly swelling, be heated to 70 DEG C-80 DEG C, insulation 30-40 minute, stir to obtain mixed liquor 1;
(2) mixed liquor is cooled to 55-60 DEG C, adds triethanolamine, and stir to obtain mixed liquor 2;
(3) mixed liquor 2 is cooled to 40-45 DEG C, Pupa bombycis active polypeptide, Herba portulacae extract, Megranate Seed P.E, tremella polysaccharide, nisin is added successively, after stirring and dissolving, keeps temperature, continues to stir 15-20 minute to obtain mixed liquor 3;
(4) mixed liquor 3 is cooled to 35 DEG C, adds Flos Rosae Rugosae quintessence oil, stirs and get final product.
Owing to containing a large amount of globulin in pupa albumen, the poorly water-soluble of globulin, directly applied in the production of cosmetics, in globulin and cosmetics, other composition mixes as wetting agent, penetrating agent etc. are very difficult, even if merge preferably with above-mentioned material, also not easily by skin is absorbed, its active component can not effectively utilize.
In addition, because part population is irritated to the pupa albumen of high protein, be applied in the production of late frost as direct by pupa albumen, its intolerance factors is not still eliminated, and the composition of skin allergy that easily causes contained in the product occurred with cosmetic forms still can continue to make skin allergy;
Therefore, in order to address this problem, pupa albumen is hydrolyzed to micromolecular polypeptide by the present invention, and only take the part that its middle-molecular-weihydroxyethyl is less than 3KDa, with the cosmetics that polypeptide form is produced, its performance obtains larger change, and water solublity strengthens greatly, is easy to commonly use adjuvant with other cosmetics and produces the cosmetics be easy to as human body skin absorbs;
In addition due to the micromolecule polypeptide after hydrolysis, be different from the high protein of original macromolecule, be the cosmetics that raw material prepares with micromolecule polypeptide, eliminate intolerance factors, use crowd no longer produces allergic phenomena;
Compared with animal derived collagen polypeptide, Pupa bombycis active polypeptide not only amino acid classes, biological activity etc. is not a halfpenny the worse, and has the advantage without cause of disease hidden danger, is applicable to being applied to skin care item;
The Megranate Seed P.E added in this product, Herba portulacae extract also have very strong antioxidant activity, the active component be rich in these two kinds of natural extracts, effectively can reduce the Degradation of Collagenase to skin collagen, thus reach maintenance skin elasticity, maintain the object of the young state of skin.
The nisin added in condensation is another kind of food-grade polypeptide product, is a kind of natural antiseptic agent, can effectively reduce because of the nutritious caused microorganism Fast-propagation of product.
The tremella polysaccharide added in condensation, be a kind of plant origin moisturizing ingredient, effectively can reduce drying, the hot injury to skin, prevent skin dehydration, also can promote the activity of SOD enzyme in Skin Cell simultaneously, play collaborative antioxidative effect with Pupa bombycis active polypeptide.
Another innovative point of the present invention is, silkworm pupa protein polypeptide is combined with Flos Rosae Rugosae quintessence oil, because pupa albumen has larger abnormal flavour, if do not eliminate abnormal flavour, is difficult to the bottleneck that breakthrough pupa albumen prepares cosmetics; Hydrolysis pupa albumen is after polypeptide, although the abnormal flavour of the overwhelming majority can be eliminated, but Pupa bombycis active polypeptide is still containing lighter fishy smell, in Flos Rosae Rugosae quintessence oil containing linalool, nerol, eugenol, methyleugenol, these materials of rose oxide can well to eliminate in silkworm pupa protein polypeptide the abnormal flavour that remains, produce the fragrance of pleasant, overcome the defect due to abnormal flavour contained in silkworm pupa protein polypeptide;
Although pupa albumen is hydrolyzed to polypeptide in addition, but this polypeptide still remains some characteristic of former albumen, such as, perishability, owing to containing bata-phenethyl alcohol and eugenol in Flos Rosae Rugosae quintessence oil, this two material has antibacterial and depressed and antiseptic property preferably, overcomes the Pupa bombycis active polypeptide defect that easily raw bacterium is perishable;
The present invention makes full use of the strong anti-oxidative activity of natural pupa albumen enzymatic hydrolysate, is applied in skin-protection product, compares in feedstuff with being applied to, and improves it and is worth, also improve its pair value added.
Beneficial effect of the present invention:
Abnormal flavour will be had, poorly water-soluble, the pupa albumen be not easy to as skin absorbs is hydrolyzed to Pupa bombycis active polypeptide, again Pupa bombycis active polypeptide is mixed mutually with other component, be prepared into after active polypeptide shines and repair condensation, overcome the defect that pupa albumen and Pupa bombycis active polypeptide itself have, condensation free from extraneous odour is repaired after preparing the solarization of gained, easily by skin is absorbed, Synergistic between multiple natural activity contained in condensation and silkworm pupa protein polypeptide, to the free radical scavenging in skin, there is significant effect, can effectively reduce Exposure to Sunlight cause the damage of biomacromolecule, reduce the melanin deposition that Exposure to Sunlight causes, sunburn, the multiple skin problem such as skin lines, reduce the probability that ultraviolet injury is carcinogenic, prevent skin dehydration simultaneously, Promote cell's growth, differentiation, propagation, keep elasticity and the skin quality of skin, there is repair after multiple solarization.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
embodiment 1
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 12%, papain is added, enzyme concentration 20000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.15KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) glycerol 8 parts, dextran sulfate 0.5 part, disodiumedetate 0.02 part, methyl parahydroxybenzoate 0.05 part are added in container, add pure water or the normal saline of 69.88 parts, stirring and dissolving, then 1 part of Acritamer 940 is added, continue to be stirred to gel-type vehicle evenly swelling, be heated to 75 DEG C, be incubated 35 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 57 DEG C, slowly adds 0.5 part of triethanolamine, stir;
(3) be cooled to 42 DEG C, successively Pupa bombycis active polypeptide 13 parts, Herba portulacae extract 1 part, Megranate Seed P.E 1 part, tremella polysaccharide 4 parts, nisin 0.05 part added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds Flos Rosae Rugosae quintessence oil 1 part, stirs, and loads glass or rustless steel container after cooling, is finished product.
The addition of pure water or normal saline gathers together enough 100 parts for standard for making whole formula.Following examples are same.
Comparative example 1
Compared with embodiment 1, Pupa bombycis active polypeptide is replaced with the pupa albumen without enzymolysis, all the other are completely the same with embodiment 1, and condensation is repaired in preparation.
Comparative example 2
Compared with embodiment 1, Pupa bombycis active polypeptide embodiment 1 prepared replaces with Pupa bombycis active polypeptide prepared by additive method, and all the other are completely the same with embodiment 1, and condensation is repaired in preparation.
Pupa bombycis active polypeptide prepared by additive method, preparation method same " Food Science " 2008, Vol 29, No.11, method described in " experimentation of silkworm pupa protein polypeptide liquid antitumor action " 588-589 page.
human body uses experimentation
1, allergic experiment
Female volunteers 60 (selecting without anaphylaxis person), age 23-40 year, be divided into three groups at random, soak one piece of gauze with distilled water or normal saline, be screwed onto half-dried, and be folded into 4 layers of about 1 square centimeter of size, cosmetics are coated in the one side of gauze, then apply and stretch on side or back normal skin at forearm, then cover 1.5 square centimeters of air-locked cellophanes or plastic sheeting, with immobilization with adhesive tape.Through the observation of 24 ~ 48 hours, if test place Pruritus or causalgia, showing that these cosmetics are irritant to skin, is then positive reaction.
Result explanation, repair condensation after shining according to the Pupa bombycis active polypeptide prepared by the present embodiment one, non-stimulated to skin gentleness, wherein contained pupa albumen is through two step enzymolysis and the small-molecular peptides prepared of ultrafiltration, anaphylactogen structure is destroyed, can not cause allergic reaction.
2, female volunteers 60 (selecting without anaphylaxis person), age 23-40 year, be divided into three groups at random, embodiment 1 group of 20 people, comparative example 1,2,3 groups of each 20 people, duration of test, stop using any external thing and/or cosmetics in test position, given the test agent and comparative example 1,2,3 sample are applied to face by experimenter respectively respectively, and morning, noon and afternoon every day are each once, continuous 12 weeks, before and after experiment starts, perusal tested crowd's sunburn quantity, carries out statistics scoring group respectively and is made up of 2 people, adopts double-blind method.
Result is as shown in the table:
Efficiently individual quantity is the user that before and after using, sunburn reduces, and uses the constant or enhancer of rear sunburn to be invalid;
Improvement degree=(before using the rear sunburn quantity of sunburn quantity-use)/use front sunburn quantity;
Efficiently individual quantity is the user that before and after using, sunburn reduces, and uses the increase of rear sunburn or constant person for invalid;
Improvement degree=(before using the rear sunburn quantity of sunburn quantity-use)/use front sunburn quantity;
In embodiment 1, after condensation is applied to skin, denseness is suitable, and the condensation that takes a morsel is smeared in the mode of looping on the back of the hand, and stranding mud phenomenon is few, not tight, comfortable after being applied to skin of face;
In comparative example 1, after condensation is applied to skin, condensation denseness is suitable, and the condensation that takes a morsel is smeared in the mode of looping on the back of the hand, has and rubs mud phenomenon with the hands, have tight sensation after being applied to skin of face; Reason may be pupa albumen compared with pupa albumen peptide, its osmotic effect and assimilation effect poor;
After being applied to skin in comparative example 2, condensation denseness is suitable, and the condensation that takes a morsel is smeared in the mode of looping on the back of the hand, and stranding mud phenomenon is few, and after being applied to face, skin has tight sense, and comfort level is slightly poor.
As can be seen from above relatively in, repair condensation after the solarization prepared for raw material with small-molecular-weight silkworm chrysalis polypeptide in the present invention, zest is little, the good absorbing effect of skin, and effectively can reduce the sunburn that ultraviolet radiation causes and produce.
embodiment 2
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 10%, papain is added, enzyme concentration 8000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.075KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) hyaluronic acid 10 parts, azone 0.5 part, disodiumedetate 0.02 part, isopropyl alcohol 0.05 part are added in container, add appropriate pure water or normal saline, stirring and dissolving, then add and then add 1 part of sodium carboxymethyl cellulose, continue to be stirred to evenly swelling, be heated to 80 DEG C, be incubated 30 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 60 DEG C, slowly adds 1 part of triethanolamine, stir;
(3) be cooled to 45 DEG C, successively 8 parts of Pupa bombycis active polypeptide, 2 parts of Herba portulacae extracts, 2 parts of Megranate Seed P.Es, 5 parts of tremella polysaccharides, 0.08 part of nisin added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds Flos Rosae Rugosae quintessence oil 2 parts, stirs, and loads glass or rustless steel container after cooling, is finished product.
embodiment 3
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 15%, papain is added, enzyme concentration 60000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.225KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) glycerol 15 parts, dextran sulfate 1 part, disodiumedetate 0.05 part, propyl p-hydroxybenzoate 0.1 part are added in container, add appropriate pure water or normal saline, stirring and dissolving, then 1.5 parts of glycan substrates are added, continue to be stirred to chitosan evenly swelling, be heated to 70 DEG C, be incubated 40 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 55 DEG C, slowly adds 1.5 parts of triethanolamine, stir;
(3) be cooled to 40 DEG C, successively Pupa bombycis active polypeptide 5 parts, Herba portulacae extract 3 parts, Megranate Seed P.E 3 parts, tremella polysaccharide 8 parts, nisin 0.1 part added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds Flos Rosae Rugosae quintessence oil 2 parts, stirs, and loads glass or rustless steel container after cooling, is finished product.
embodiment 4
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 10%, papain is added, enzyme concentration 30000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.2KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) Trivent OCG 10 parts, dextran sulfate 0.5 part, disodiumedetate 0.03 part, propylene glycol 0.07 part are added in container, add appropriate pure water or normal saline, stirring and dissolving, then 1.5 parts of sodium carboxymethyl cellulose are added, continue to be stirred to gel-type vehicle evenly swelling, be heated to 80 DEG C, be incubated 30 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 60 DEG C, slowly adds 0.5 part of triethanolamine, stir;
(3) be cooled to 45 DEG C, successively 10 parts of Pupa bombycis active polypeptide, 2 parts of Herba portulacae extracts, 1 part of Megranate Seed P.E, 5 parts of tremella polysaccharides, 0.08 part of nisin added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds 1.5 parts of Flos Rosae Rugosae quintessence oils, stirs, and loads glass or rustless steel container after cooling, is finished product.
embodiment 5
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 13%, papain is added, enzyme concentration 450000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.125KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) 8 parts of glucosan glycosides, 1 part of azone, 0.01 part of disodiumedetate, 0.05 part of methyl parahydroxybenzoate are added in container, add appropriate pure water or normal saline, stirring and dissolving, then 1 part of Acritamer 940,0.5 part of sodium carboxymethyl cellulose is added, continue to be stirred to gel-type vehicle evenly swelling, be heated to 75 DEG C, be incubated 35 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 57 DEG C, slowly adds triethanolamine, stir;
(3) be cooled to 42 DEG C, successively Pupa bombycis active polypeptide, Herba portulacae extract, Megranate Seed P.E, tremella polysaccharide, nisin added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds Flos Rosae Rugosae quintessence oil, stirs, and loads glass or rustless steel container after cooling, is finished product.
embodiment 6
(1) preparation of Pupa bombycis active polypeptide:
(1) with the pupa albumen solution of deionized water compound concentration 11%, papain is added, enzyme concentration 10000IU/g pupa albumen, hydrolysis temperature 55 DEG C, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(2), after first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add Esperase protease, enzyme concentration 0.1KNPU/g pupa albumen, enzymolysis 3h, hydrolysis temperature 55 DEG C, 92 DEG C of enzyme denaturing 13min after enzymolysis completes;
(3) the material filtering removing insoluble matter after enzyme denaturing, obtain faint yellow clear transparent solutions, this polypeptide solution crosses supermicro filtration membrane, and collecting molecular weight is the filtrate of below 3000Da;
(4) gained polypeptide solution adds 4% activated carbon adsorption decolouring, and after macroporous resin DA201-C desalting processing, vacuum concentration, lyophilization obtains Pupa bombycis active polypeptide, for subsequent use.
(2) Pupa bombycis active polypeptide repairs the preparation of condensation after shining
(1) poly-glycosaminoglycan 10 parts, 5 parts, lecithin, azone 0.5 part, disodiumedetate 0.02 part, methyl parahydroxybenzoate 0.02 part, propyl p-hydroxybenzoate 0.06 part are added in container, add appropriate pure water or normal saline, stirring and dissolving, then 0.5 part of Acritamer 940,1 part of chitosan is added, continue to be stirred to gel-type vehicle evenly swelling, be heated to 75 DEG C, be incubated 35 minutes, stir;
(2) above-mentioned mixed liquor is cooled to 57 DEG C, slowly adds triethanolamine, stir;
(3) be cooled to 42 DEG C, successively 8 parts of Pupa bombycis active polypeptide, 2 parts of Herba portulacae extracts, 2 parts of Megranate Seed P.Es, 4 parts of tremella polysaccharides, 0.05 part of nisin added, after stirring and dissolving, keep temperature, continue stirring 17 minutes;
(4) mixed liquor is cooled to 35 DEG C, adds 2 parts of Flos Rosae Rugosae quintessence oils, stirs, and loads glass or rustless steel container after cooling, is finished product.