CN105687040A

CN105687040A - Multifunctional emulsion containing algae hydrolysates and collagen and preparation method of emulsion

Info

Publication number: CN105687040A
Application number: CN201610128836.9A
Authority: CN
Inventors: 付晓婷; 刘楠楠; 裴方欣
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2016-02-29
Filing date: 2016-02-29
Publication date: 2016-06-22
Anticipated expiration: 2036-02-29
Also published as: CN105687040B

Abstract

The invention discloses a multifunctional emulsion containing algae hydrolysates and collagen. The multifunctional emulsion is prepared from 1,3-butanediol, glycerol, polysorbate-20, panthenol, hexadecanol, octadecanol, tocopherol, allantoin, hyaluronic acid, ethylenediamine tetraacetic acid disodium, sweet almond oil, squalane, octyldodecanol, lemon essential oil, algae hydrolysates, fish scale collagen protein and triethanolamine. The invention further discloses a preparation method of the multifunctional emulsion. According to the multifunctional emulsion, hydrolysates of macroalgae and fish scale collagen protein are taken as main active ingredients and matched with active ingredients such as hyaluronic acid, tocopherol, squalane, allantoin and the like, the mixture and cosmetic ground substances are mixed and homogenized, and the multifunctional emulsion is obtained. The multifunctional emulsion has the advantages of being safe, mild, capable of whitening skin, moisturizing skin and preventing wrinkles and ageing and free from stimulation, cannot damage skin, can protect skin and moisturize the skin and further has the efficacy of skin whitening, wrinkle resisting and ageing prevention.

Description

A kind of multi-functional emulsion containing Sargassum zymolyte and collagen protein and preparation method thereof

Technical field

The present invention relates to a kind of cosmetic technical field, particularly relate to a kind of for multi-functional emulsion containing Sargassum zymolyte and collagen protein and preparation method thereof。

Background technology

Along with the development of society, the progress of medical science and the improvement of quality of life, people start to focus on the maintenance of skin。The air pollution aggravated gradually due to modern society and the life stress day by day increased, the skin of more and more people is polluted and the invasion and attack of internal free radical by external environment condition, the series of symptoms such as obscure, dry, sensitive, old and feeble occurs。Along with the attention degree of skin nursing problem is increased by people, in the program of daily skin nursing, emulsion is requisite important step。Emulsion is to have the liquid frost class cosmetics of good skin-moisturizing, skin effect, emulsion on the market at present, often add too much chemicals and preservative etc., although can reach the short time improves the purpose of skin, but skin can be made to produce dependency or skin is had intense stimulus。Therefore, a kind of add natural marine organism active substance, healthy non-stimulated emulsion can be subject to liking of consumer。

Marine bioactivity extract, because of himself excellent biocompatibility, conclusive moistening water conservation effect and clear and definite biological activity, has been subject to the attention of scientific research personnel and the favor on beauty treatment circle。The marine biomaterial such as collagen protein, phenols, oligosaccharide now successfully develop into all kinds of cosmetics, but the multi-functional emulsion containing Sargassum zymolyte and collagen protein still belongs to blank。Collagen protein is a kind of structural protein being present in intercellular substance, it is distributed widely in animal connective tissue, have no cytotoxicity, regulatable biodegradation rate, can special promotion or suppress the plurality of advantages such as the biochemistry compatibility is excellent of the interaction of cell-material, physiological condition。Although at present existing patent of invention replaces pig, Corii Bovis seu Bubali as the new raw material extracting collagen protein using marine organism materials such as fish skin, there is higher biological safety and excellent biological characteristics, but the fishy smell of fish skin is big, de-taste technology wastes time and energy, so fish scale becomes the new material extracting collagen protein。

At present, although the preparation method that existing patent of invention discloses Sargassum extract and collagen protein, and point out that it can be used for cosmetics, but do not use the Sargassum extract of Enzymatic Extraction full nutrition more, such as: application number be 201310257256.6 Chinese patent disclose a kind of Sargassum essence and take thing functional latex, in this emulsion, Sargassum essence takes thing and does not use the more active component of Enzymatic Extraction。Also having patent of invention to replace pig, Corii Bovis seu Bubali as the new raw material extracting collagen protein using marine organism materials such as fish skin, but the fishy smell of fish skin is big, removing sulfuldioxide is wasted time and energy, so this patent uses fish scale to become the material extracting collagen protein。Application number be 201010553485.9 Chinese patent disclose a kind of marine fish collagen moisturizing anti-aging cosmetics, it is simply that the extraction material adopting fish skin to be collagen protein, but the fishy smell of fish skin is relatively big, extraction cost is higher。

Therefore, cosmetic technical field need badly provide a kind of safe, gentle, efficiently, not only will not damage skin, the multi-functional emulsion containing Sargassum zymolyte and collagen protein of whitening, effect crease-resistant, anti-aging can also be reached while protection moisturizing。

Summary of the invention

In order to solve the problems referred to above, the present invention provide a kind of safe, gentle, efficiently, not only will not damage skin, the multi-functional emulsion containing Sargassum zymolyte and collagen protein of whitening, effect crease-resistant, anti-aging can also be reached while protection moisturizing。

The technical solution used in the present invention is: a kind of multi-functional emulsion containing Sargassum zymolyte and collagen protein, including following component: 1,3 butylene glycol, glycerol, polysorbate-20, pantothenylol, hexadecanol, octadecanol, tocopherol, allantoin, hyaluronic acid, disodiumedetate, Semen pruni armeniacae oil, squalane, octyldodecanol, Fructus Citri Limoniae quintessence oil, Sargassum zymolyte, fish scale collagen, triethanolamine, deionized water；

Further, each components based on weight percentage: 1, 3-butanediol 1%-10%, glycerol 1%-10%, polysorbate-201%-6%, pantothenylol 0.5%-5%, hexadecanol 0.5%-4%, octadecanol 0.5%-4%, tocopherol 0.1%-4%, allantoin 0.05%-4%, hyaluronic acid 0.05%-6%, disodiumedetate 0.05%-2%, Semen pruni armeniacae oil 1%-10%, squalane 1%-8%, octyldodecanol 0.5%-4%, Fructus Citri Limoniae quintessence oil 0.05%-2%, Sargassum zymolyte 0.01%-10%, fish scale collagen 0.01%-10%, surplus is triethanolamine and deionized water；

Further, each components based on weight percentage: 1,3-butanediol 5%, glycerol 5%, polysorbate-204%, pantothenylol 1%, hexadecanol 1%, octadecanol 1%, tocopherol 0.5%, allantoin 0.2%, hyaluronic acid 0.1%, disodiumedetate 0.1%, Semen pruni armeniacae oil 3%, squalane 3%, octyldodecanol 2%, Fructus Citri Limoniae quintessence oil 0.1%, Sargassum zymolyte 2%, fish scale collagen 0.5%, surplus is triethanolamine and deionized water；

Further, each components based on weight percentage: 1,3-butanediol 7%, glycerol 8%, polysorbate-204%, pantothenylol 2%, hexadecanol 1.5%, octadecanol 1.5%, tocopherol 1%, allantoin 0.5%, hyaluronic acid 0.3%, disodiumedetate 0.1%, Semen pruni armeniacae oil 5%, squalane 2%, octyldodecanol 4%, Fructus Citri Limoniae quintessence oil 0.05%, Sargassum zymolyte 1%, fish scale collagen 1%, surplus is triethanolamine and deionized water；

Further, each components based on weight percentage；1,3-butanediol 3%, glycerol 10%, polysorbate-206%, pantothenylol 5%, hexadecanol 0.5%, octadecanol 0.5%, tocopherol 4%, allantoin 1%, hyaluronic acid 3%, disodiumedetate 0.5%, Semen pruni armeniacae oil 5%, squalane 5%, octyldodecanol 4%, Fructus Citri Limoniae quintessence oil 0.5%, Sargassum zymolyte 0.5%, fish scale collagen 0.5%, surplus is triethanolamine and deionized water。

The invention also discloses the preparation method containing Sargassum zymolyte and the multi-functional emulsion of collagen protein as above, concrete steps include:

1) preparation of Sargassum zymolyte: after Sargassum is rinsed well, dry, pulverize to obtain algae powder, add the ratio of the enzyme of 500-1000U/g and the water of 100mL according to 1g algae powder, algae powder, enzyme are mixed homogeneously with water, maintains the temperature at 30-60 DEG C, extract 6-24 hour under oscillating condition, the centrifugal 15min when 4000-8000r/min, after sucking filtration, rotation is steamed, and obtains Sargassum zymolyte after lyophilizing；

2) preparation of fish scale collagen: after the cleaning of fresh fish scale water, remove impurity, it is the solid-liquid ratio of 1: 30 by fish scale and 0.2mol/LEDTA solution according to w/v, soak 24h decalcification, it is the solid-liquid ratio of 1: 8 with 0.1mol/L sodium hydroxide solution according to w/v again, soak 6h and remove noncollagen protein, by through decalcification and unless the fish scale of collagen protein adds 2000U/g pepsin, it is the solid-liquid ratio of 1: 18 with the citric acid solution of 2-10% according to w/v, soaks 24-48h and extract；Collagen protein extracting solution is obtained through 300 order filter-cloth filterings, adding sodium chloride in extracting solution and reach 0.9mol/L to its concentration, saltout 24h, is then centrifuged for obtaining collagen deposit thing, putting the precipitate in dialysis 24h in the bag filter that trapped molecular weight is 10kD, lyophilizing is standby；

3) preparation of multi-functional emulsion: successively by 1,3-butanediol, glycerol, polysorbate-20, pantothenylol, hexadecanol, octadecanol, tocopherol, allantoin, hyaluronic acid, disodiumedetate mix by weight percentage, add in the deionized water by the surplus obtained after each weight of material percentage calculation, heat under stirring condition to 70-75 DEG C, so as to be completely dissolved, obtain aqueous-phase material；

Another container is sequentially added under Semen pruni armeniacae oil by weight percentage, squalane, octyldodecanol stirring condition and is heated to 70-75 DEG C so that it is be fused into liquid, obtain oil phase thing；

Oil phase thing is joined in aqueous-phase material, is cooled to after 40-50 DEG C and adds Fructus Citri Limoniae quintessence oil by weight percentage, Sargassum zymolyte and fish scale collagen, use blender high-speed stirred；

Regulate emulsion pH to 5.0-6.0, homogenizer homogenizing 5-60min with triethanolamine, be cooled to room temperature；

Emulsion is ageing 1-3 days at room temperature, obtain the multi-functional emulsion of product after fill；

Further, step 1) described in Sargassum be the combination of one or more Sargassums in Brown algae, red algae, chlorella；

Further, step 1) described in enzyme be the combination of one or more of alkaline protease, cellulase, pentosan compound enzyme, cellulose complex enzyme。

Beneficial effects of the present invention:

Relative to the cosmetic emulsion containing a large amount of chemicals and preservative on the market, the multi-functional emulsion containing Sargassum zymolyte and collagen protein of the present invention is by attaching the active substance containing Sargassum zymolyte and collagen protein, solve the problem using chemicals and preservative easily to produce to stimulate, by the polyphenol in interpolation zymolyte and oligosaccharide, the fish scale collagen etc. that auxiliary is added, make emulsion Middle nutrition composition more comprehensive available, will not to skin irritant, and consumer will not be made to produce the problems such as dependency, safety, gentle, efficiently, not only will not damage skin, whitening can also be reached while protection moisturizing, crease-resistant, anti-aging effect。

The present invention selects the tangleweed of pure natural and fish scale to be primary raw material, Extraction Processes is adopted to extract the various bioactivators in Sargassum, and from fish scale, extract collagen protein, fishy smell is few, extraction cost is low, these natural active matters can be absorbed by the skin rapidly, will not bring burden and safety, gentleness to skin, do not cause skin allergy, make the multi-functional emulsion of the present invention have whitening, moisturizing, crease-resistant, defying age and repair the effects such as impaired skin yet。And containing natural antibacterial in Sargassum zymolyte, antibacterial effect is good, it is not necessary to add chemical preservative, nontoxic non-stimulated, suitable in the crowd of different sexes, age and skin quality, cost performance is high, environmental protection, preparation is simple for it, and production cost is low, is suitable for industrialized production。

Detailed description of the invention

Embodiment 1:

1, form the composition of multifunctional cosmetics emulsion of the present invention and weight content be:

A phase: 1,3 butylene glycol (5%), glycerol (5%), polysorbate-20 (4%), pantothenylol (1%), hexadecanol (1%), octadecanol (1%), tocopherol (0.5%), allantoin (0.2%), hyaluronic acid (0.1%), disodiumedetate (0.1%)；

B phase: Semen pruni armeniacae oil (3%), squalane (3%), octyldodecanol (2%), Fructus Citri Limoniae quintessence oil (0.1%)；

C phase: Sargassum zymolyte (2%), fish scale collagen (0.5%)；

D phase: triethanolamine regulates pH to 5.5。

2, technical process:

1) preparation of described Sargassum zymolyte: after Sargassum fusiforme (Harv.) Setch is cleaned up, dry, pulverize to obtain algae powder, the water of 1g algae powder is added 500U/g alkaline protease and 100mL is mixed homogeneously, and maintains the temperature at 45 DEG C, reacts 12h under oscillating condition, 4000rpm is centrifuged 15min, supernatant sucking filtration, rotary evaporation after sucking filtration, prepare Sargassum fusiforme (Harv.) Setch zymolyte after lyophilization；

2) preparation of described collagen protein: after the cleaning of fresh morrhua fish scale water, remove impurity, by fish scale with 0.2mol/LEDTA solution according to 1: 30 (w/v) solid-liquid ratio, soaks 24h decalcification；Again with 0.1mol/L sodium hydroxide solution according to 1: 8 (w/v) solid-liquid ratio, soak 6h and remove noncollagen protein；By through decalcification and unless the fish scale of collagen protein adds 2000U/g pepsin, with 6% citric acid solution according to the solid-liquid ratio of 1: 18 (w/v), soak 24h and extract；Obtaining collagen protein extracting solution through 300 order filter-cloth filterings, add sodium chloride and reach 0.9mol/L to its concentration in extracting solution, saltout 24h, is then centrifuged for obtaining collagen deposit thing；Put the precipitate in dialysis 24h, the fish scale collagen that lyophilizing is extracted in the bag filter that trapped molecular weight is 10kD；

3) preparation of multi-functional emulsion: heating A phase and B phase temperature to 75 DEG C respectively, under stirring condition, is added in A phase, homogenizing 5min by B；Stirring is cooled to less than 45 DEG C, each for C phase component is joined in A, B phase mixture；With triethanolamine regulation system pH to 5.5, under room temperature, ageing prepared the multi-functional emulsion containing Sargassum zymolyte and collagen protein after 3 days。

Embodiment 2:

A phase: 1,3 butylene glycol (7%), glycerol (8%), polysorbate-20 (4%), pantothenylol (2%), hexadecanol (1.5%), octadecanol (1.5%), tocopherol (1%), allantoin (0.5%), hyaluronic acid (0.3%), disodiumedetate (0.1%)；

B phase: Semen pruni armeniacae oil (5%), squalane (2%), octyldodecanol (4%), Fructus Citri Limoniae quintessence oil (0.05%)；

C phase: Sargassum zymolyte (1%), fish scale collagen (1%)；

D phase: triethanolamine regulates pH to 5.0。

2, technical process:

1) preparation of described Sargassum zymolyte: after Scytosiphon lomentarius (Lyngh.)J. Ag. is cleaned up, dry, pulverize to obtain algae powder, the water of 1g algae powder is added 800U/g cellulase and 100mL is mixed homogeneously, and maintains the temperature at 50 DEG C, reacts 6h under oscillating condition, 6000rpm is centrifuged 15min, supernatant sucking filtration, rotary evaporation after sucking filtration, prepare Scytosiphon lomentarius (Lyngh.)J. Ag. zymolyte after lyophilization；

2) preparation of described collagen protein: fresh grass carp scales water cleans, after remove impurity, by fish scale with 0.2mol/LEDTA solution according to 1: 30 (w/v) solid-liquid ratio, soak 24h decalcification, again with 0.1mol/L sodium hydroxide solution according to 1: 8 (w/v) solid-liquid ratio, soak 6h and remove noncollagen protein, by through decalcification with unless the fish scale of collagen protein adds 2000U/g pepsin, with 4% citric acid solution according to the solid-liquid ratio of 1: 18 (w/v), soak 32h to extract, collagen protein extracting solution is obtained through 300 order filter-cloth filterings, in extracting solution, add sodium chloride reach 0.9mol/L to its concentration, saltout 24h, it is then centrifuged for obtaining collagen deposit thing, put the precipitate in dialysis 24h in the bag filter that trapped molecular weight is 10kD, the fish scale collagen that lyophilizing is extracted；

3) preparation of multi-functional emulsion: heating A phase and B phase temperature to 75 DEG C respectively, under stirring condition, is added in A phase, homogenizing 20min by B；Stirring is cooled to less than 45 DEG C, each for C phase component is joined in A, B phase mixture；With triethanolamine regulation system pH to 5.0, under room temperature, ageing prepared the multi-functional emulsion containing Sargassum zymolyte and collagen protein after 3 days。

Embodiment 3:

A phase: 1,3 butylene glycol (3%), glycerol (10%), polysorbate-20 (6%), pantothenylol (5%), hexadecanol (0.5%), octadecanol (0.5%), tocopherol (4%), allantoin (1%), hyaluronic acid (3%), disodiumedetate (0.5%)；

B phase: Semen pruni armeniacae oil (5%), squalane (5%), octyldodecanol (4%), Fructus Citri Limoniae quintessence oil (0.5%)；

C phase: Sargassum zymolyte (0.5%), fish scale collagen (0.5%)；

D phase: triethanolamine regulates pH to 6.0。

2, technical process:

1) preparation of described Sargassum zymolyte: after many ribs algae is cleaned up, dry, pulverize to obtain algae powder, the water of 1g algae powder is added 1000U/g enzyme and 100mL is mixed homogeneously, and maintains the temperature at 40 DEG C, reacts 6h under oscillating condition, 8000rpm is centrifuged 15min, supernatant sucking filtration, rotary evaporation after sucking filtration, prepare many ribs algae enzymolysis activity material after lyophilization；

2) preparation of described collagen protein: after the cleaning of fresh Hypophthalmichthys molitrix squama ice pure water, remove impurity, by fish scale with 0.2mol/LEDTA solution according to 1: 30 (w/v) solid-liquid ratio, soak 24h decalcification, again with 0.1mol/L sodium hydroxide solution according to 1: 8 (w/v) solid-liquid ratio, soak 6h and remove noncollagen protein, by through decalcification with unless the fish scale of collagen protein adds 2000U/g pepsin, with 2% citric acid solution according to the solid-liquid ratio of 1: 18 (w/v), soak 48h and extract；Collagen protein extracting solution is obtained through 300 order filter-cloth filterings, in extracting solution, add sodium chloride reach 0.9mol/L to its concentration, saltout 24h, it is then centrifuged for obtaining collagen deposit thing, putting the precipitate in dialysis 24h in the bag filter that trapped molecular weight is 10kD, lyophilizing is the fish scale collagen extracted；

3) preparation of multi-functional emulsion: heating A phase and B phase temperature to 75 DEG C respectively, under stirring condition, is added in A phase by B, and homogenizing 60min, stirring is cooled to less than 45 DEG C, each for C phase component is sequentially added in A, B phase mixture；With triethanolamine regulation system pH to 6.0, under room temperature, ageing prepared the multi-functional emulsion containing Sargassum zymolyte and collagen protein after 3 days。

Embodiment 4:

1, the activity rating of Sargassum zymolyte:

Table 1

2, the emulsion inhibitory action to tryrosinase:

Table 2

Sample	Tyrosinase inhibition rate
		Embodiment 1	20.44%
Embodiment 2	22.31%
		Embodiment 3	15.04%

3, the oxidation resistance of emulsion:

Table 3

Sample	DPPH clearance rate	Scavenging action to hydroxyl free radical	Superoxide anion clearance rate
				Embodiment 1	75.23%	40.12%	60.11%
Embodiment 2	78.89%	41.21%	61.54%
				Embodiment 3	70.22%	38.03%	57.79%

4, the moisture absorption of emulsion and performance of keeping humidity:

Table 4

Sample	Moisturizing rate
		Embodiment 1	90.13%
Embodiment 2	92.01%
		Embodiment 3	88.36%

5, the sense organ of emulsion, physicochemical property detect:

Table 5

6, above-mentioned test result shows, the emulsion of the present invention has good tyrosinase inhibitory action after adding Sargassum zymolyte and removes DPPH, the ability of hydroxy radical and superoxide anion and moisture absorption, performance of keeping humidity, fish scale collagen is coordinated to add in emulsion, more enhance the functional effect of emulsion, not only can effectively suppress tryrosinase, scavenging free radicals, full restoration nutrition skin can also be played, improve the self-protection ability of skin, the emulsion making the present invention has moisturizing, nutrition, whitening, antiinflammatory, the multi-efficiencies such as reparation, it is carried out sense organ, physicochemical property detects, discovery meets national standard, it is qualified products。

Above-described embodiment, simply to illustrate that the technology of the present invention is conceived and feature, its objective is to be in that to allow one of ordinary skilled in the art will appreciate that present disclosure and to implement according to this, can not limit the scope of the invention with this。The change of every equivalence done by the essence of present invention or modification, all should be encompassed in protection scope of the present invention。

Claims

1. the multi-functional emulsion containing Sargassum zymolyte and collagen protein, it is characterized in that, including following component: 1,3 butylene glycol, glycerol, polysorbate-20, pantothenylol, hexadecanol, octadecanol, tocopherol, allantoin, hyaluronic acid, disodiumedetate, Semen pruni armeniacae oil, squalane, octyldodecanol, Fructus Citri Limoniae quintessence oil, Sargassum zymolyte, fish scale collagen, triethanolamine, deionized water。

2. multi-functional emulsion as claimed in claim 1, it is characterized in that, each components based on weight percentage: 1, 3-butanediol 1%-10%, glycerol 1%-10%, polysorbate-201%-6%, pantothenylol 0.5%-5%, hexadecanol 0.5%-4%, octadecanol 0.5%-4%, tocopherol 0.1%-4%, allantoin 0.05%-4%, hyaluronic acid 0.05%-6%, disodiumedetate 0.05%-2%, Semen pruni armeniacae oil 1%-10%, squalane 1%-8%, octyldodecanol 0.5%-4%, Fructus Citri Limoniae quintessence oil 0.05%-2%, Sargassum zymolyte 0.01%-10%, fish scale collagen 0.01%-10%, surplus is triethanolamine and deionized water。

3. multi-functional emulsion as claimed in claim 2, it is characterized in that, each components based on weight percentage: 1,3-butanediol 5%, glycerol 5%, polysorbate-204%, pantothenylol 1%, hexadecanol 1%, octadecanol 1%, tocopherol 0.5%, allantoin 0.2%, hyaluronic acid 0.1%, disodiumedetate 0.1%, Semen pruni armeniacae oil 3%, squalane 3%, octyldodecanol 2%, Fructus Citri Limoniae quintessence oil 0.1%, Sargassum zymolyte 2%, fish scale collagen 0.5%, surplus is triethanolamine and deionized water。

4. multi-functional emulsion as claimed in claim 2, it is characterized in that, each components based on weight percentage: 1,3-butanediol 7%, glycerol 8%, polysorbate-204%, pantothenylol 2%, hexadecanol 1.5%, octadecanol 1.5%, tocopherol 1%, allantoin 0.5%, hyaluronic acid 0.3%, disodiumedetate 0.1%, Semen pruni armeniacae oil 5%, squalane 2%, octyldodecanol 4%, Fructus Citri Limoniae quintessence oil 0.05%, Sargassum zymolyte 1%, fish scale collagen 1%, surplus is triethanolamine and deionized water。

5. multi-functional emulsion as claimed in claim 2, it is characterized in that, each components based on weight percentage: 1,3-butanediol 3%, glycerol 10%, polysorbate-206%, pantothenylol 5%, hexadecanol 0.5%, octadecanol 0.5%, tocopherol 4%, allantoin 1%, hyaluronic acid 3%, disodiumedetate 0.5%, Semen pruni armeniacae oil 5%, squalane 5%, octyldodecanol 4%, Fructus Citri Limoniae quintessence oil 0.5%, Sargassum zymolyte 0.5%, fish scale collagen 0.5%, surplus is triethanolamine and deionized water。

6. the preparation method containing Sargassum zymolyte and the multi-functional emulsion of collagen protein as according to any one of claim 1-5, it is characterised in that including:

Emulsion is ageing 1-3 days at room temperature, obtain the multi-functional emulsion of product after fill。

7. the preparation method of multi-functional emulsion as claimed in claim 6, it is characterised in that step 1) described in Sargassum be the combination of one or more Sargassums in Brown algae, red algae, chlorella。

8. the preparation method of multi-functional emulsion as claimed in claim 6, it is characterised in that step 1) described in enzyme be the combination of one or more of alkaline protease, cellulase, pentosan compound enzyme, cellulose complex enzyme。