CN103450908B - A kind of soil improvement agent of control of plant bacterial wilt and application thereof - Google Patents
A kind of soil improvement agent of control of plant bacterial wilt and application thereof Download PDFInfo
- Publication number
- CN103450908B CN103450908B CN201310385917.3A CN201310385917A CN103450908B CN 103450908 B CN103450908 B CN 103450908B CN 201310385917 A CN201310385917 A CN 201310385917A CN 103450908 B CN103450908 B CN 103450908B
- Authority
- CN
- China
- Prior art keywords
- soil
- bacterial wilt
- yeast
- tomato
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
A kind of soil improvement agent prescription of control of plant bacterial wilt and application thereof, be aided with mineral powder by yeast residue to mix, wherein the mass ratio of yeast residue and mineral powder is 8:1-19:1, using said composition can control of plant bacterial wilt effectively, its preventive effect can reach more than 45-90%, and can postpone morbidity 5-19 days.
Description
Technical field
The present invention relates to Agro-ecology field, be specially for the technology of bacterial wilt of tomato control, particularly relate to a kind of soil improvement agent prescription and application thereof of control of plant bacterial wilt.
Background technology
Bacterial wilt of tomato (Bacterialwiltoftomato) be a kind of by the withered Raul Salmonella of green grass or young crops (
ralstoniasolanacearum) the destructive bacillary soil-borne disease that causes.This disease is common, Yi Fa and propagating rapidly, has a strong impact on the yield and quality of vegetables, even causes to lose in the generation time and receive.Simultaneously because tomato economic worth is high, the huge market demand, long-term continuous cropping makes Soil degradation, Quality Down, and then bacterial wilt takes place frequently, wide by disease area, causes tremendous economic to lose.Bacterial wilt prevention and controls main is now breeding for disease resistance, changes tillage method, chemical pesticide control, Antagonistic Fungi control, nutrition regulation etc., and their prevention effect all has some limitations at present.Thus Ecological preventive way has become the focus of research gradually, and wherein the research of soil improvement agent is one of important research direction.
In recent years, utilizing soil improvement agent to improve soil quality and repairing the soil of degenerating becomes study hotspot both domestic and external, and it has promotion soil nutrient balance, improves soil microorganisms structure, improves the plurality of advantages such as quality of agricultural product.Soil improvement agent is divided into natural amendments, synthesis modifying agent, natural-synthetic copolymer modifying agent and biological modification agent by raw material sources, and all kinds of soil improvement agent is applied to the control of bacterial wilt of tomato by existing research.Li etc. use mineral powder in greenhouse tomato test, and bacterial wilt preventive effect reaches 89.99%.The research such as Saikia finds that the fusaric acid that Zn and Cu mineral powder modifying agent can produce by reducing pathogenic bacteria strengthens biocontrol microorganisms
pseudomonasfluorescensthe activity of 4-92, improves the prevention effect of garbanzo blight.Amino acid, carbohydrate and other biological active substance is rich in yeast residue.Mineral powder, as a kind of natural mineral, can improve Soil structure, improves preserve moisture and fertility ability.But single soil improvement agent is difficult to obtain desirable control effects, the mixing of dissimilar modifying agent is joined and executes, be expected to the soil improvement agent prescription obtaining control of plant bacterial wilt.
Summary of the invention
the technical problem solved
The present invention is directed to the problem that bacterial wilt of tomato control is difficult, provide a kind of soil improvement agent and application thereof of control of plant bacterial wilt.
technical schemea kind of soil improvement agent of control of plant bacterial wilt, be aided with mineral powder by yeast residue to mix, the mass ratio of yeast residue and mineral powder is 8:1-19:1, the mixture that described mineral powder is a kind of pulverizing by natural mineral and produces is that 1:3:2:1:1 mixes by quartz, biotite, potassium felspar sand, plagioclase and peridotites according to mass ratio.
Described yeast residue is the discarded yeast residue that yeast factory produces yeast.
The application of the soil improvement agent of described control of plant bacterial wilt, gets tomato seeds, sterilizes with the NaClO of 2%wt ~ 2.5%wt, and soak seed at 55 DEG C, in the incubator of 25 DEG C, vernalization 48h, broadcasts cave the seed of vernalization and coil, and waters Hoagland'sSolution nutritive medium; Seedling grow to two leaves wholeheartedly time, mix with the mass ratio of yeast residue by 8:1-19:1 without powder with by ore deposit, mix thoroughly respectively with soil, the amount of application of formula is the 0.5%wt of soil, uses 2.67gkg
-1composite fertilizer, in described composite fertilizer, the ratio of N P and K is N-P
2o
5-K
2o=15%-15%-15%, after stirring evenly with mixing with soil, growth after transplanting for tomato.
beneficial effect
At bacterial wilt of tomato onset peak period, the present invention can reach more than 45-90% to bacterial wilt preventive effect, and can postpone morbidity 5-19 days.
Accompanying drawing explanation
Fig. 1 is the tomato plant situation of control group morbidity;
Fig. 2 is for using tomato plant of the present invention.
Embodiment
Following embodiment does not limit technical scheme of the present invention in any form, and the technical scheme that the mode that every employing is equal to replacement or equivalent transformation obtains all drops on protection scope of the present invention.
embodiment 1:
The research in our early stage finds to utilize mineral powder and fertilizer, and according to mass ratio, for 45-60% ︰ 40-55%, evenly mixed to impose in soil can effectively preventing bacterial wilt of tomato, its prevention effect is more than 89%, replace fertilizer with waste yeast powder in the present invention, regulate the composition of mineral nutrients to realize the object controlling bacterial wilt simultaneously.Because containing rich in protein, amino acid, VITAMIN and trace element in yeast powder, and containing the growth-stimulating factor of digestive ferment with some the unknowns, base has higher biological activity, can promote that soil beneficial microorganism grows, and strengthens the resistance of plant.Can also realize the recycling of waste, so control bacterial wilt of tomato using yeast powder as main adding material in this composition, embodiment is as follows simultaneously:
Yeast residue and mineral powder are pressed the mass ratio mixing of 8:1, amount of application when mixing thoroughly with soil is 0.5% of soil quality, the mixture that mineral powder is a kind of pulverizing by natural mineral and produces is that 1:3:2:1:1 mixes by quartz, biotite, potassium felspar sand, plagioclase and peridotites according to mass ratio.
Get pure tomato seeds, sterilize with the NaClO of 2%wt ~ 2.5%wt, soak seed at 55 DEG C, pull out after cool in water, vernalization 48h in the incubator of 25 DEG C, the seed of vernalization is broadcast to cave coil in (specification 32 hole, seedling medium is vermiculite), periodically water Hoagland'sSolution nutritive medium.Seedling grow to two leaves wholeheartedly time, soil improvement agent is administered in soil, simultaneously every kilogram of soil Zhong Shi 2.67g composite fertilizer (N-P in composite fertilizer
2o
5-K
2o=15%wt-15%wt-15%wt), after stirring evenly with process mixing with soil, growth after transplanting for tomato.Tomato seedling is transplanted in above-mentioned soil.When seedling grows to 5 ~ 6 true leaves (after the 20d that transplants seedlings), adopt and hinder root perfusion inoculation Ralstonia solanacearum, the concentration of having been diluted by sterilized water is 1 × 10
8cfumL
-1bacterium liquid water around root.Observe the incidence of different treatment every day, record bacterial wilt disease index respectively, calculate modifying agent to the preventive effect of bacterial wilt of tomato.
Known by the incidence of bacterial wilt under continuous recording different treatment, wilting symptom is there is in the tomato in control group from 4 days, show the phenomenon of morbidity, and soil improvement agent process, incidence significantly will be lighter than contrast, it is 46.2% to the preventive effect of bacterial wilt of tomato, and can postpone morbidity 5 days.
embodiment 2:
Key step is identical with embodiment 1, be distinguished as and yeast residue, mineral powder are mixed by the mass ratio of 9:1, mix thoroughly with soil, the amount of application of formula is 0.5% of soil quality, the mixture that mineral powder is a kind of pulverizing by natural mineral and produces is that 1:3:2:1:1 mixes by quartz, biotite, potassium felspar sand, plagioclase and peridotites according to mass ratio.Tomato seedling grow to two leaves wholeheartedly time, soil improvement agent prescription is administered in soil, uses 2.67gkg simultaneously
-1composite fertilizer, to (after the 20d that transplants seedlings) during 5 ~ 6 true leaves, adopts and hinders root perfusion inoculation Ralstonia solanacearum.
The modifying agent made according to this combination is 98.3% to the preventive effect of bacterial wilt of tomato, and compared with the control, can postpone morbidity 19 days.
Found by the quantity detecting Ralstonia solanacearum in soil, in soil handled by the modifying agent of this combination, the contrast of its number ratio decreases 96.3%, and the growth of using soil improvement agent and can suppress pathogenic bacteria is described, thus reduce the incidence probability of plant, play antibacterial simultaneously and press down sick effect.Simultaneously compared with the control, the thick and plant height of the leaf area of tomato plant in the present invention, stem all significantly increases
The different modifying agent process of table 1 is on the impact of tomato growth
| Process | Leaf area (mm 2） | Stem thick (mm) | Plant height (cm) | Pathogenic bacteria quantity (CFU/g) |
| Contrast | 117.1±19.7 | 4.6±0.4 | 13.9±1.1 | 1.50×10 6 |
| The present invention | 158.6±5.1 | 5.8±0.2 | 14.4±0.4 | 5.55×10 4 |
embodiment 3:
Key step is identical with embodiment 1, be distinguished as and yeast residue, mineral powder are mixed by the mass ratio of 19:1, mix thoroughly with soil, the amount of application of formula is 0.5% of soil quality, the mixture that mineral powder is a kind of pulverizing by natural mineral and produces is that 1:3:2:1:1 mixes by quartz, biotite, potassium felspar sand, plagioclase and peridotites according to mass ratio.Tomato seedling grow to two leaves wholeheartedly time, soil improvement agent prescription is administered in soil, uses 2.67gkg simultaneously
-1composite fertilizer, to (after the 20d that transplants seedlings) during 5 ~ 6 true leaves, adopts and hinders root perfusion inoculation Ralstonia solanacearum.
At onset peak period, be 58.0% to the preventive effect of bacterial wilt of tomato, and morbidity can be postponed 3 days.
Claims (1)
1. the application of the soil improvement agent of control of plant bacterial wilt, is characterized in that getting tomato seeds, sterilizes with the NaClO of 2%wt ~ 2.5%wt, soak seed at 55 DEG C, in the incubator of 25 DEG C, vernalization 48h, broadcasts cave the seed of vernalization and coils, and waters Hoagland'sSolution nutritive medium; Seedling grow to two leaves wholeheartedly time, yeast residue, mineral powder press the mass ratio mixing of 9:1, mix thoroughly with soil, the amount of application of formula is 0.5% of soil quality, the mixture that described mineral powder is a kind of pulverizing by natural mineral and produces is that 1:3:2:1:1 mixes by quartz, biotite, potassium felspar sand, plagioclase and peridotites according to mass ratio; Described yeast residue is the discarded yeast residue that yeast factory produces yeast; Use 2.67gkg
-1composite fertilizer, in described composite fertilizer, the ratio of N P and K is N-P
2o
5-K
2o=15%-15%-15%, after stirring evenly with mixing with soil, growth after transplanting for tomato.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385917.3A CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385917.3A CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103450908A CN103450908A (en) | 2013-12-18 |
| CN103450908B true CN103450908B (en) | 2016-02-24 |
Family
ID=49733752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310385917.3A Active CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103450908B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106540283A (en) * | 2016-10-14 | 2017-03-29 | 华南农业大学 | A kind of soil anaerobic sterilization of control of plant bacterial wilt |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609979B (en) * | 2015-02-04 | 2017-10-24 | 北京东方力原生物科技有限公司 | A kind of microbial-bacterial fertilizer for improveing salt affected soil and preparation method thereof |
| CN108384550B (en) * | 2018-01-22 | 2020-11-03 | 南京林业大学 | Preparation method of soil conditioner |
| CN114854627B (en) * | 2022-04-29 | 2023-10-13 | 重庆西农植物保护科技开发有限公司 | Pseudomonas fluorescens for preventing and treating bacterial wilt and application thereof |
-
2013
- 2013-08-29 CN CN201310385917.3A patent/CN103450908B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 《不同土壤改良剂对番茄青枯病的防治效果》;刘肖肖等;《农业环境科学学报》;20130720;第32卷(第7期);第1368－1374页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106540283A (en) * | 2016-10-14 | 2017-03-29 | 华南农业大学 | A kind of soil anaerobic sterilization of control of plant bacterial wilt |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103450908A (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| US20170073279A1 (en) | Facility secondary no3- salinized soil modifier, preparation method and modification method | |
| CN103449886A (en) | Composite microbial fertilizer for paddy | |
| CN104496573B (en) | A kind of grape special biological and ecological methods to prevent plant disease, pests, and erosion composite microbiological fertilizer, production method and application | |
| CN103382139A (en) | Agaricus bisporus (Lange) Sing culture medium | |
| CN102432356A (en) | Method for producing specialized organic fertilizer for protected cultivation by silkworm excrement fermentation | |
| CN104447127A (en) | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof | |
| CN107099473A (en) | A kind of water-soluble antimicrobial polypeptide bacterial manure of full nutrition and its production method | |
| CN105565970A (en) | Novel multifunctional compound bio-fertilizer and preparation method thereof | |
| CN103450908B (en) | A kind of soil improvement agent of control of plant bacterial wilt and application thereof | |
| US20180327330A1 (en) | Organic Fertilizer for Vegetables and Its Preparation Method | |
| CN108640759A (en) | A kind of preparation method of organic fertilizer for the sweetened expelling parasite of melon and fruit | |
| CN108029501A (en) | A kind of vegetable nutrient diseases prevention seedling medium and preparation method and application | |
| CN103772011A (en) | Special compound biological liquid-state bacterial fertilizer for grain | |
| WO2022022204A1 (en) | Soil microbial regulator for enhancing plant stress resistance | |
| CN101812410B (en) | Streptomycesvinaceus-drappus microbial inoculum and method for preparing same | |
| CN107746303A (en) | It is a kind of using xylose residue as decomposed matrix of raw material and its preparation method and application | |
| CN107285953A (en) | Composite zymocyte, biological organic fertilizer and preparation method thereof | |
| CN105175159A (en) | Production method of rice seedling dry raising and strengthening biological medium and application thereof | |
| CN107721602A (en) | A kind of alkaline Sandy Soil improvement matrix and preparation method thereof | |
| CN108046893A (en) | A kind of Chinese medicine slag biological fertilizer and preparation method thereof | |
| CN106717428A (en) | The tea garden fertilizing method that a kind of organic fertilizer is arranged in pairs or groups with composite fertilizer | |
| CN109354552A (en) | A kind of biological organic fertilizer and preparation method thereof | |
| CN107602278A (en) | The preparation method of tobacco breeding and seedling nursing with equipment matrix | |
| CN109111928A (en) | A kind of preparation method of soil-repairing agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |