CN103450908A - Soil conditioner for preventing and controlling tomato bacterial wilt and application thereof - Google Patents
Soil conditioner for preventing and controlling tomato bacterial wilt and application thereof Download PDFInfo
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- CN103450908A CN103450908A CN2013103859173A CN201310385917A CN103450908A CN 103450908 A CN103450908 A CN 103450908A CN 2013103859173 A CN2013103859173 A CN 2013103859173A CN 201310385917 A CN201310385917 A CN 201310385917A CN 103450908 A CN103450908 A CN 103450908A
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- bacterial wilt
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- tomato
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 31
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 28
- 239000003516 soil conditioner Substances 0.000 title abstract 3
- 240000003768 Solanum lycopersicum Species 0.000 title description 27
- 239000011707 mineral Substances 0.000 claims abstract description 25
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 241000227653 Lycopersicon Species 0.000 claims abstract 4
- 239000002689 soil Substances 0.000 claims description 47
- 239000003795 chemical substances by application Substances 0.000 claims description 29
- 230000006872 improvement Effects 0.000 claims description 19
- 241000196324 Embryophyta Species 0.000 claims description 13
- 239000003337 fertilizer Substances 0.000 claims description 10
- 239000002131 composite material Substances 0.000 claims description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- 229910052626 biotite Inorganic materials 0.000 claims description 5
- 229910052655 plagioclase feldspar Inorganic materials 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000010453 quartz Substances 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000000050 nutritive effect Effects 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 230000003111 delayed effect Effects 0.000 abstract 1
- 235000010755 mineral Nutrition 0.000 description 19
- 230000003449 preventive effect Effects 0.000 description 7
- 238000011160 research Methods 0.000 description 6
- 241000589771 Ralstonia solanacearum Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000010412 perfusion Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- DGMPVYSXXIOGJY-UHFFFAOYSA-N Fusaric acid Chemical compound CCCCC1=CC=C(C(O)=O)N=C1 DGMPVYSXXIOGJY-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 244000045195 Cicer arietinum Species 0.000 description 1
- 235000010523 Cicer arietinum Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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Abstract
The invention discloses a soil conditioner for preventing and controlling tomato bacterial wilt and application thereof. The soil conditioner is prepared by mixing yeast residues with mineral powder, wherein the mass ratio of the yeast residues to the mineral powder is (8:1) to (19:1). By applying the composition, the tomato bacterial wilt can be effectively prevented and controlled; the control effect can be up to over 45-90%; the morbidity can be delayed by 5-19 days.
Description
Technical field
The present invention relates to the Agro-ecology field, is the technology of preventing and treating for bacterial wilt of tomato specially, relates in particular to a kind of soil improvement agent prescription and application thereof of control of plant bacterial wilt.
Background technology
Bacterial wilt of tomato (Bacterial wilt of tomato) be a kind of by the withered Raul Salmonella of green grass or young crops (
ralstonia solanacearum) the destructive bacillary soil-borne disease that causes.This disease is common, Yi Fa and propagating rapidly, has a strong impact on the yield and quality of vegetables, even causes to lose and receives the time occurs.Simultaneously because the tomato economic worth is high, the huge market demand, long-term continuous cropping makes Soil degradation, Quality Down, and then bacterial wilt takes place frequently, and is subject to the disease area wide, causes the tremendous economic loss.The main bacterial wilt method of preventing and treating is breeding for disease resistance, changes tillage method, chemical pesticide control, Antagonistic Fungi control, nutrition regulation etc. now, and their prevention effect all has some limitations at present.Thereby Ecological preventive way become the focus of research gradually, wherein the research of soil improvement agent is one of important research direction.
In recent years, utilizing soil improvement agent to improve soil quality and repairing the soil of degenerating becomes study hotspot both domestic and external, and it has the soil nutrient balance of promotion, improve the soil microorganisms structure, improve the plurality of advantages such as quality of agricultural product.Soil improvement agent is divided into natural modifying agent, synthetic modifying agent, natural-synthetic copolymer modifying agent and biological modification agent by raw material sources, and existing research is applied to all kinds of soil improvement agents the control of bacterial wilt of tomato.Li etc. use mineral powder in the greenhouse tomato test, and the bacterial wilt preventive effect reaches 89.99%.The researchs such as Saikia discovery Zn and Cu mineral powder modifying agent can be strengthened biocontrol microorganisms by the fusaric acid that reduces the pathogenic bacteria generation
pseudomonas fluorescensthe activity of 4-92, the prevention effect of raising garbanzo blight.Be rich in amino acid, carbohydrate and other biological active substance in the yeast residue.Mineral powder, as a kind of natural mineral, can improve Soil structure, improves the preserve moisture and fertility ability.But single soil improvement agent is difficult to obtain the desirable effect of controlling, and dissimilar modifying agent is mixed to join and execute, and is expected to obtain the soil improvement agent prescription of control of plant bacterial wilt.
Summary of the invention
the technical problem solved
The present invention is directed to bacterial wilt of tomato and prevent and treat difficult problem, a kind of soil improvement agent and application thereof of control of plant bacterial wilt are provided.
technical schemea kind of soil improvement agent of control of plant bacterial wilt, being aided with mineral powder by the yeast residue mixes, the mass ratio of yeast residue and mineral powder is 8:1-19:1, described mineral powder is a kind of mixture produced by the natural mineral pulverizing, by quartz, biotite, potassium felspar sand, plagioclase and peridotites, according to mass ratio, is that 1:3:2:1:1 mixes.
Described yeast residue is the discarded yeast residue that yeast factory produces yeast.
The application of the soil improvement agent of described control of plant bacterial wilt, get tomato seeds, with the NaClO sterilization of 2%wt ~ 2.5%wt, seed soaking under 55 ℃, in the incubator of 25 ℃, vernalization 48 h, broadcast cave to the seed of vernalization and coil, and waters Hoagland's Solution nutritive medium; Seedling grows to two leaves wholeheartedly the time, and ore deposit is mixed with the mass ratio of yeast residue by 8:1-19:1 without powder, with soil, mixes thoroughly respectively, and the 0.5%wt that the amount of application of formula is soil, use 2.67 gkg
-1composite fertilizer, in described composite fertilizer, the ratio of nitrogen phosphorus potassium is N-P
2o
5-K
2o=15%-15%-15%, after stirring evenly with mixing with soil, growth after transplanting for tomato.
beneficial effect
At the bacterial wilt of tomato onset peak period, more than the present invention can reach 45-90% to the bacterial wilt preventive effect, and can postpone morbidity 5-19 days.
The accompanying drawing explanation
The tomato plant situation that Fig. 1 is the control group morbidity;
Fig. 2 is for being used tomato plant of the present invention.
Embodiment
Following embodiment does not limit technical scheme of the present invention in any form, and every employing is equal to replaces or technical scheme that the mode of equivalent transformation obtains all drops on protection scope of the present invention.
embodiment 1:
The research in our early stage finds to utilize mineral powder and fertilizer, and according to mass ratio, for 45-60% ︰ 40-55%, evenly mixed impose in soil can the effectively preventing bacterial wilt of tomato, its prevention effect is more than 89%, replace fertilizer with the waste yeast powder in the present invention, the composition of simultaneously regulating the mineral nutrient realizes controlling the purpose of bacterial wilt.Because contain rich in protein, amino acid, VITAMIN and trace element in yeast powder, and contain digestive ferment and some unknown growth-stimulating factors, base has higher biological activity, can promote the growth of soil beneficial microorganism, strengthens the resistance of plant.Simultaneously can also realize the recycling of waste, so using yeast powder in this composition, as main interpolation material, control bacterial wilt of tomato, embodiment is as follows:
The mass ratio of yeast residue and mineral powder being pressed to 8:1 mixes, amount of application while mixing thoroughly with soil is 0.5% of soil quality, mineral powder is a kind of mixture produced by the natural mineral pulverizing, by quartz, biotite, potassium felspar sand, plagioclase and peridotites, according to mass ratio, is that 1:3:2:1:1 mixes.
Get pure tomato seeds, NaClO sterilization with 2%wt ~ 2.5%wt, seed soaking under 55 ℃, after cool in water, pull out, vernalization 48 h in the incubator of 25 ℃, the seed of vernalization is broadcast to cave and coil (specification 32 holes, seedling medium is vermiculite), regularly water as required Hoagland's Solution nutritive medium.Seedling grows to two leaves wholeheartedly the time, and soil improvement agent is administered in soil, executes 2.67g composite fertilizer (N-P in composite fertilizer in every kilogram of soil simultaneously
2o
5-K
2o=15%wt-15%wt-15%wt), after stirring evenly with the processing mixing with soil, growth after transplanting for tomato.Tomato seedling is transplanted in above-mentioned soil.When seedling grows to 5 ~ 6 true leaves (after 20 d that transplant seedlings), adopt and hinder root perfusion inoculation Ralstonia solanacearum, the concentration that sterilized water has been diluted is 1 * 10
8cfumL
-1bacterium liquid water around root.Observe the incidence of different treatment every day, record respectively the bacterial wilt disease index, calculate the preventive effect of modifying agent to bacterial wilt of tomato.
Incidence by bacterial wilt under the continuous recording different treatment is known, the wilting symptom appearred in the tomato in control group since 4 days, show the phenomenon of morbidity, and soil improvement agent is processed, incidence will significantly be lighter than contrast, its preventive effect to bacterial wilt of tomato is 46.2%, and can postpone morbidity 5 days.
embodiment 2:
Key step is identical with embodiment 1, difference mixes for the mass ratio of yeast residue, mineral powder being pressed to 9:1, with soil, mix thoroughly, the amount of application of formula is soil quality 0.5%, mineral powder is a kind of mixture produced by the natural mineral pulverizing, by quartz, biotite, potassium felspar sand, plagioclase and peridotites, according to mass ratio, is that 1:3:2:1:1 mixes.Tomato seedling is grown to two leaves wholeheartedly the time, and the soil improvement agent prescription is administered in soil, uses 2.67 gkg simultaneously
-1composite fertilizer, during to 5 ~ 6 true leaves (after 20 d that transplant seedlings), adopt and hinder root perfusion inoculation Ralstonia solanacearum.
The modifying agent of making according to this combination is 98.3% to the preventive effect of bacterial wilt of tomato, and compared with the control, can postpone morbidity 19 days.
By the quantity that detects Ralstonia solanacearum in soil, find, in the handled soil of the modifying agent of this combination, its quantity has reduced 96.3% than contrast, illustrates and uses the growth that soil improvement agent can suppress pathogenic bacteria, thereby reduce the incidence probability of plant, play antibacterial and but sick effect simultaneously.Simultaneously compared with the control, the leaf area of tomato plant in the present invention, stem is thick and plant height all significantly increases
The different modifying agents of table 1 are processed the impact on tomato growth
| Process | Leaf area (mm 2） | Stem thick (mm) | Plant height (cm) | Pathogenic bacteria quantity (CFU/g) |
| Contrast | 117.1±19.7 | 4.6±0.4 | 13.9±1.1 | 1.50×10 6 |
| The present invention | 158.6±5.1 | 5.8±0.2 | 14.4±0.4 | 5.55×10 4 |
embodiment 3:
Key step is identical with embodiment 1, difference mixes for the mass ratio of yeast residue, mineral powder being pressed to 19:1, with soil, mix thoroughly, the amount of application of formula is soil quality 0.5%, mineral powder is a kind of mixture produced by the natural mineral pulverizing, by quartz, biotite, potassium felspar sand, plagioclase and peridotites, according to mass ratio, is that 1:3:2:1:1 mixes.Tomato seedling is grown to two leaves wholeheartedly the time, and the soil improvement agent prescription is administered in soil, uses 2.67 gkg simultaneously
-1composite fertilizer, during to 5 ~ 6 true leaves (after 20 d that transplant seedlings), adopt and hinder root perfusion inoculation Ralstonia solanacearum.
At onset peak period, be 58.0% to the preventive effect of bacterial wilt of tomato, and can postpone morbidity 3 days.
Claims (3)
1. the soil improvement agent of a control of plant bacterial wilt, it is characterized in that being aided with mineral powder by the yeast residue mixes, the mass ratio of yeast residue and mineral powder is 8:1-19:1, described mineral powder is a kind of mixture produced by the natural mineral pulverizing, by quartz, biotite, potassium felspar sand, plagioclase and peridotites, according to mass ratio, is that 1:3:2:1:1 mixes.
2. the soil improvement agent of control of plant bacterial wilt according to claim 1, is characterized in that described yeast residue is the discarded yeast residue that yeast factory produces yeast.
3. the application of the soil improvement agent of claim 1 or 2 described control of plant bacterial wilt, it is characterized in that getting tomato seeds, NaClO sterilization with 2%wt ~ 2.5%wt, seed soaking under 55 ℃, vernalization 48 h in the incubator of 25 ℃, the seed of vernalization is broadcast to cave and coil, water Hoagland's Solution nutritive medium; Seedling grows to two leaves wholeheartedly the time, and ore deposit is mixed with the mass ratio of yeast residue by 8:1-19:1 without powder, with soil, mixes thoroughly respectively, and the 0.5%wt that the amount of application of formula is soil, use 2.67 gkg
-1composite fertilizer, in described composite fertilizer, the ratio of nitrogen phosphorus potassium is N-P
2o
5-K
2o=15%-15%-15%, after stirring evenly with mixing with soil, growth after transplanting for tomato.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385917.3A CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385917.3A CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103450908A true CN103450908A (en) | 2013-12-18 |
| CN103450908B CN103450908B (en) | 2016-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310385917.3A Expired - Fee Related CN103450908B (en) | 2013-08-29 | 2013-08-29 | A kind of soil improvement agent of control of plant bacterial wilt and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609979A (en) * | 2015-02-04 | 2015-05-13 | 北京东方力原生物科技有限公司 | Microbial fertilizer for improving saline alkali soil and preparation method thereof |
| CN106540283A (en) * | 2016-10-14 | 2017-03-29 | 华南农业大学 | A kind of soil anaerobic sterilization of control of plant bacterial wilt |
| CN108384550A (en) * | 2018-01-22 | 2018-08-10 | 南京林业大学 | A kind of preparation method of soil conditioner |
| CN114854627A (en) * | 2022-04-29 | 2022-08-05 | 重庆西农植物保护科技开发有限公司 | Pseudomonas fluorescens for preventing and treating bacterial wilt and application thereof |
-
2013
- 2013-08-29 CN CN201310385917.3A patent/CN103450908B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609979A (en) * | 2015-02-04 | 2015-05-13 | 北京东方力原生物科技有限公司 | Microbial fertilizer for improving saline alkali soil and preparation method thereof |
| CN106540283A (en) * | 2016-10-14 | 2017-03-29 | 华南农业大学 | A kind of soil anaerobic sterilization of control of plant bacterial wilt |
| CN108384550A (en) * | 2018-01-22 | 2018-08-10 | 南京林业大学 | A kind of preparation method of soil conditioner |
| CN108384550B (en) * | 2018-01-22 | 2020-11-03 | 南京林业大学 | Preparation method of soil conditioner |
| CN114854627A (en) * | 2022-04-29 | 2022-08-05 | 重庆西农植物保护科技开发有限公司 | Pseudomonas fluorescens for preventing and treating bacterial wilt and application thereof |
| CN114854627B (en) * | 2022-04-29 | 2023-10-13 | 重庆西农植物保护科技开发有限公司 | Pseudomonas fluorescens for preventing and treating bacterial wilt and application thereof |
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| Publication number | Publication date |
|---|---|
| CN103450908B (en) | 2016-02-24 |
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