CN103431163B - Artificial feed for chrysopa perla larvae and preparation method of artificial feed - Google Patents
Artificial feed for chrysopa perla larvae and preparation method of artificial feed Download PDFInfo
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- CN103431163B CN103431163B CN201310400386.0A CN201310400386A CN103431163B CN 103431163 B CN103431163 B CN 103431163B CN 201310400386 A CN201310400386 A CN 201310400386A CN 103431163 B CN103431163 B CN 103431163B
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Abstract
The invention discloses artificial feed for chrysopa perla larvae. The artificial feed comprises the following components in parts by weight: 20-40 parts of tussah pupa complete pulp, 10-30 parts of eggs, 20-40 parts of beer yeast powder, 10-25 parts of sucrose, 1-5 parts of trehalose, 0.1-0.5 part of vitamin C and 0.1-0.3 part of potassium sorbate. The artificial feed are rich in sources of raw materials and rich in various nutrient substances, and can be used for completely replacing mealybugs or rice moth eggs to meet the eating demand of the chrysopa perla larvae. The prepared artificial feed for chrysopa perla larvae is rich in nutrition, easy and convenient to prepare, and economical in price, saves time and effort, can be used for the large-scale breeding of chrysopa perla, and has a very good effect.
Description
Technical field:
The invention belongs to cultured insect field, be specifically related to man-made feeds of a kind of Chrysopa larva and preparation method thereof.
Background technology:
Chrysopa is the Important Natural Enemy of the multiple agriculture and forestry injurious insects such as aphid, aleyrodid, mite class, Lepidoptera ovum and larva, is a kind of natural enemy insect that has using value in biological control of insect pests.Adopting indoor feeding technology is amount reproduction natural enemy, improve the important channel of prevention effect, and the preparation of man-made feeds is keys of natural enemy multiplication technique.At present, indoor feeding Chrysopa is mainly with aphid, mealybug, rice moth egg etc.And a large amount of research has also been done both at home and abroad in man-made feeds aspect, mainly using fly maggot powder, pork liver, beef, beef liver, soya bean or Soy hydrolysate as main component.
In the operating technology > > of document < < artificial feeding Chinese green lacewing control cotton pest, the composition of larva artificial feed has honey and water.In the artificial avette feed First Report of Studies > > of document < < Chrysopa sinica larvae, the composition of larva artificial feed has soya bean, honey and water.In the research > > of document < < Chrysopa man-made feeds, larva artificial feed has roe powder, honey and water.And other documents are used full-cream fly maggot powder, degreasing fly maggot powder, pork liver powder, fresh beef, fresh beef liver or Soy hydrolysate as main component.But these man-made feeds culture effect are not fine, and cost compare is high.
Summary of the invention:
The object of this invention is to provide a kind of culture effect good, composition is simple, man-made feeds of Chrysopa larva with low cost and preparation method thereof.
The man-made feeds of Chrysopa larva of the present invention, it is characterized in that, meter, comprises that tussah chrysalis starches 20-40 part, egg 10-30 part, beer yeast powder 20-40 part, sucrose 10-25 part, trehalose 1-5 part, vitamin C 0.1-05 part and potassium sorbate 0.1-0.3 part entirely by weight.
Described Chrysopa larva is yellow agate Chrysopa larva.
Described egg is raw egg, comprises egg yolk and egg.
Described beer yeast powder is the brewer's yeast with Fructus Hordei Germinatus brew, and itself is any beer composition of tool not.
The man-made feeds of Chrysopa larva of the present invention are prepared by the following method, and the method comprises the following steps:
The shell of tussah chrysalis is removed, and water is cleaned tussah chrysalis, is positioned in warm water and soaks, and then removes wherein intestines, and remaining grinding, obtains tussah chrysalis and entirely starch;
According to the raw material of the man-made feeds of above-mentioned Chrysopa larva and proportioning thereof, by tussah chrysalis slurry, egg, beer yeast powder, sucrose, trehalose, vitamin C, potassium sorbate mixing and stirring entirely, obtain the man-made feeds of Chrysopa.
Described being positioned in warm water soaked, and its soak time is 6～10min, and water temperature is 60 ± 1 ℃.
The present invention, than prior art, has following beneficial effect:
Raw material sources of the present invention are abundant, are rich in multiple nutrients material, can substitute the eating requirements that mealybug or rice moth egg meet Chrysopa larva completely.The man-made feeds of the Chrysopa larva that obtains of preparation, it is nutritious, prepare easy, price economy, time saving and energy saving, can be used for the extensive raising of Chrysopa, and effect is very good.
The specific embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
First with scissors, the shell of tussah chrysalis is cut, with clear water tussah chrysalis wash clean, being positioned over water-bath soaks 6 minutes, soaking water temperature is 60 ± 1 ℃, then takes out, and tussah chrysalis is cut off to an osculum, in intestines extrude and remove, with cooking machine, by remaining, fully grind again, obtain tussah chrysalis and entirely starch, take afterwards tussah chrysalis and entirely starch 30g; With mortar, VITAMIN C TABLET (Vit C contents is 6.2%, mass fraction) is ground brokenly, take 3.2g; Afterwards egg is shelled, with agitator, stir, then take 15g; Then take beer yeast powder 30g, sucrose 15g, trehalose 2g, potassium sorbate 0.2g, finally all the components weighing up is poured in agitator and fully mixed thoroughly, obtain the man-made feeds of described Chrysopa larva.
At the beginning of incubate first-instar young and be put in culture dish, each culture dish is put 1, provides and is moistened with the moistening cotton of water and sufficient man-made feeds, records its length of time and death condition every day until it is pupated.10 larvas are a repetition, repeat five times.Control experiment is usingd mealybug as feed.
The yellow agate Chrysopa larva of raising with mealybug, its first-instar young survival rate 80.8%, second instar larvae survival rate 81.9%, percentage of pupation (being third-instar larvae survival rate) 89.5%, the yellow agate Chrysopa larva of raising with man-made feeds, its first-instar young survival rate 86.7%, second instar larvae survival rate 85.1%, percentage of pupation 93.3%.
Embodiment 2
First with scissors, the shell of tussah chrysalis is cut, with clear water tussah chrysalis wash clean, being positioned over water-bath soaks 9 minutes, soaking water temperature is 60 ± 1 ℃, then takes out, and tussah chrysalis is cut off to an osculum, in intestines extrude and remove, with cooking machine, it is fully ground again, obtain tussah chrysalis and entirely starch, take afterwards tussah chrysalis and entirely starch 40g; With mortar, VITAMIN C TABLET (Vit C contents is 6.2%, mass fraction) is ground brokenly, take 8g; Afterwards egg is shelled, with agitator, stir, then take 30g; Then take beer yeast powder 40g, sucrose 25g, trehalose 5g, potassium sorbate 0.3g, finally all the components weighing up is poured in agitator and fully mixed thoroughly, obtain the man-made feeds of described Chrysopa larva.
At the beginning of incubate first-instar young and be put in culture dish, each culture dish is put 1, provides and is moistened with the moistening cotton of water and sufficient man-made feeds, records its length of time and death condition every day until it is pupated.20 larvas are a repetition, repeat five times.Control experiment is usingd mealybug as feed.
The yellow agate Chrysopa larva of raising with mealybug, its first-instar young survival rate 80.3%, second instar larvae survival rate 80.5%, percentage of pupation 89.7%, the yellow agate Chrysopa larva of raising with man-made feeds, its first-instar young survival rate 90.6%, second instar larvae survival rate 86.7%, percentage of pupation 95.6%.
Embodiment 3
First with scissors, the shell of tussah chrysalis is cut, with clear water tussah chrysalis wash clean, being positioned over water-bath soaks 10 minutes, soaking water temperature is 60 ± 1 ℃, then takes out, and tussah chrysalis is cut off to an osculum, in intestines extrude and remove, with cooking machine, it is fully ground again, obtain tussah chrysalis and entirely starch, take afterwards tussah chrysalis and entirely starch 20g; With mortar, VITAMIN C TABLET (Vit C contents is 6.2%, mass fraction) is ground brokenly, take 1.6g; Afterwards egg is shelled, with agitator, stir, then take 10g; Then take beer yeast powder 20g, sucrose 10g, trehalose 1g, potassium sorbate 0.1g, finally all the components weighing up is poured in agitator and fully mixed thoroughly, obtain the man-made feeds of described Chrysopa larva.
At the beginning of incubate first-instar young and be put in culture dish, each culture dish is put 1, provides and is moistened with the moistening cotton of water and sufficient man-made feeds, records its length of time and death condition every day until it is pupated.30 larvas are a repetition, in triplicate.Control experiment is usingd mealybug as feed.
The yellow agate Chrysopa larva of raising with mealybug, its first-instar young survival rate 78.3%, second instar larvae survival rate 82.2%, percentage of pupation (being third-instar larvae survival rate) 88.4%, the yellow agate Chrysopa larva of raising with man-made feeds, its first-instar young survival rate 82.2%, second instar larvae survival rate 88.7%, percentage of pupation (being third-instar larvae survival rate) 90.3%.
Embodiment 4
Formula of the present invention (formula 1) and another formula (filling a prescription 2) are raised to yellow agate Chrysopa as man-made feeds respectively to be contrasted.
Formula 1:
First with scissors, the shell of tussah chrysalis is cut, with clear water tussah chrysalis wash clean, being positioned over water-bath soaks 10 minutes, soaking water temperature is 60 ± 1 ℃, then takes out, and tussah chrysalis is cut off to an osculum, in intestines extrude and remove, with cooking machine, it is fully ground again, obtain tussah chrysalis and entirely starch, take afterwards tussah chrysalis and entirely starch 40g; With mortar, VITAMIN C TABLET (Vit C contents is 6.2%, mass fraction) is ground brokenly, take 4.84g; Afterwards egg is shelled, with agitator, stir, then take 20g; Then take beer yeast powder 30g, sucrose 15g, trehalose 5g, potassium sorbate 0.3g, finally all the components weighing up is poured in agitator and fully mixed thoroughly, obtain the man-made feeds of described Chrysopa larva, for filling a prescription 1 man-made feeds.
Formula 2:
Be 6.2% by raw egg 40g, beer yeast powder 30g, honey 20g, sucrose 10g, vitamin C 1.6g(Vit C contents, mass fraction), water 100ml, see above-mentioned batching mixing and stirring, 2 man-made feeds obtain filling a prescription.
At the beginning of incubate first-instar young and be put in culture dish, each culture dish is put 1, provides and is moistened with the moistening cotton of water and sufficient man-made feeds (formula 1 or formula 2), records its length of time and death condition every day until it is pupated.30 larvas are a repetition, in triplicate.
The yellow agate Chrysopa larva of raising with formula 1, its first-instar young survival rate 86.7%, second instar larvae survival rate 91.7%, percentage of pupation (being third-instar larvae survival rate) 90.9%, the yellow agate Chrysopa larva of raising with formula 2, its first-instar young survival rate 80.0%, second instar larvae survival rate 83.2%, percentage of pupation (being third-instar larvae survival rate) 82.1%.
Embodiment 5
Formula 1:
First with scissors, the shell of tussah chrysalis is cut, with clear water tussah chrysalis wash clean, being positioned over water-bath soaks 10 minutes, soaking water temperature is 60 ± 1 ℃, then takes out, and tussah chrysalis is cut off to an osculum, in intestines extrude and remove, with cooking machine, it is fully ground again, obtain tussah chrysalis and entirely starch, take afterwards tussah chrysalis and entirely starch 30g; With mortar, VITAMIN C TABLET (Vit C contents is 6.2%, mass fraction) is ground brokenly, take 3.23g; Afterwards egg is shelled, with agitator, stir, then take 15g; Then take beer yeast powder 30g, sucrose 12g, trehalose 3g, potassium sorbate 0.2g, finally all the components weighing up is poured in agitator and fully mixed thoroughly, obtain the man-made feeds of described Chrysopa larva, for filling a prescription 1 man-made feeds.
Formula 2: tussah chrysalis is starched entirely
At the beginning of incubate first-instar young and be put in culture dish, each culture dish is put 1, provides and is moistened with the moistening cotton of water and sufficient man-made feeds (formula 1 or formula 2), records its length of time and death condition every day until it is pupated.30 larvas are a repetition, in triplicate.
The yellow agate Chrysopa larva of raising with formula of the present invention, its first-instar young survival rate 83.3%, second instar larvae survival rate 93.7%, percentage of pupation (being third-instar larvae survival rate) 89.9%, the yellow agate Chrysopa larva of supporting with the full paste feeding of tussah chrysalis, its first-instar young survival rate 23.3%, second instar larvae survival rate 28.5%, percentage of pupation (being third-instar larvae survival rate) 0.0%.
Claims (4)
1. man-made feeds of a Chrysopa larva, it is characterized in that, count by weight, tussah chrysalis is starched 20-40 part, egg 10-30 part, beer yeast powder 20-40 part, sucrose 10-25 part, trehalose 1-5 part, vitamin C 0.1-05 part and potassium sorbate 0.1-0.3 part entirely.
2. the man-made feeds of Chrysopa larva according to claim 1, is characterized in that, described Chrysopa larva is yellow agate Chrysopa larva.
3. a preparation method for the man-made feeds of Chrysopa larva claimed in claim 1, is characterized in that, comprises the following steps:
The shell of tussah chrysalis is removed, and water is cleaned tussah chrysalis, is positioned in warm water and soaks, and then removes wherein intestines, and remaining grinding, obtains tussah chrysalis and entirely starch;
According to the raw material of the man-made feeds of Chrysopa larva claimed in claim 1 and proportioning thereof, by tussah chrysalis slurry, egg, beer yeast powder, sucrose, trehalose, vitamin C, potassium sorbate mixing and stirring entirely, obtain the man-made feeds of Chrysopa larva.
4. preparation method according to claim 3, is characterized in that, described being positioned in warm water soaked, and its soak time is 6～10min, and water temperature is 60 ± 1 ℃.
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| CN105746435B (en) * | 2016-02-25 | 2018-05-11 | 北京市农林科学院 | A kind of method using rice moth egg breeding chrysopa septempunctata larva |
| CN108208326A (en) * | 2018-04-09 | 2018-06-29 | 华南农业大学 | A kind of man-made feeds formula of Huang agate Chrysopa |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102960310A (en) * | 2012-11-27 | 2013-03-13 | 中国农业科学院植物保护研究所 | Breeding method and special feed for adult chrysopa septempunctata |
| CN103039720A (en) * | 2012-11-27 | 2013-04-17 | 中国农业科学院植物保护研究所 | Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960310A (en) * | 2012-11-27 | 2013-03-13 | 中国农业科学院植物保护研究所 | Breeding method and special feed for adult chrysopa septempunctata |
| CN103039720A (en) * | 2012-11-27 | 2013-04-17 | 中国农业科学院植物保护研究所 | Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae |
Non-Patent Citations (3)
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| 于彩虹等.海藻糖—昆虫的血糖.《昆虫知识》.2008,第45卷(第5期),第832-836页. * |
| 叶正楚等.中华草蛉人工饲料的研究.《植物保护学报》.1979,第6卷(第2期),第11-16页. * |
| 李志飞等.丽草蛉幼虫人工饲料的研究.《热带作物学报》.2013,第34卷(第3期),第547-550页. * |
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